JP2015108622A - マイクロ流体センサ - Google Patents
マイクロ流体センサ Download PDFInfo
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- JP2015108622A JP2015108622A JP2014233308A JP2014233308A JP2015108622A JP 2015108622 A JP2015108622 A JP 2015108622A JP 2014233308 A JP2014233308 A JP 2014233308A JP 2014233308 A JP2014233308 A JP 2014233308A JP 2015108622 A JP2015108622 A JP 2015108622A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/49—Blood
- G01N33/4905—Determining clotting time of blood
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
- G01N27/3272—Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/12—Specific details about manufacturing devices
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0627—Sensor or part of a sensor is integrated
- B01L2300/0645—Electrodes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0887—Laminated structure
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/16—Surface properties and coatings
- B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
- G01N2333/974—Thrombin
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Hematology (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Biochemistry (AREA)
- Clinical Laboratory Science (AREA)
- Dispersion Chemistry (AREA)
- Ecology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Electrochemistry (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Description
表3
14 スペーサ層
16 電極層
18 貫通孔
20 通気孔
22 付着領域
24 流路
26 入口領域
28 反応ゾーン
30 電極付着物
301 送信電極
302 受信電極
32 絶縁体基材
Claims (18)
- マイクロ流体センサであって、
カバー層と、
基材と、該基材の上に形成され、10ミクロン(μm)未満の厚さの少なくとも1つの電極とを含む電極層と、
流体を受け入れるように構成された少なくとも1つの流路が形成された、前記カバー層と前記電極層との間のスペーサ層と、
を備えるマイクロ流体センサ。 - 前記少なくとも1つの電極は、送信電極及び受信電極を備える、請求項1に記載のマイクロ流体センサ。
- 前記送信電極及び前記受信電極は、前記送信電極と前記受信電極との間で、前記少なくとも1つの流路の流体のインピーダンスを測定するように構成された、請求項2に記載のマイクロ流体センサ。
- 前記少なくとも1つの電極は、スクリーン印刷を用いて前記基材の上に形成される、請求項1、2、又は3に記載のマイクロ流体センサ。
- 前記少なくとも1つの流路の高さは、50μmから100μmの間である、請求項1から4のいずれか1項に記載のマイクロ流体センサ。
- 前記カバー層は、前記少なくとも1つの流路と界接する複数の貫通孔を備える、請求項1から5のいずれか1項に記載のマイクロ流体センサ。
- 前記カバー層は、試薬が付着される複数の領域を更に備える、請求項6に記載のマイクロ流体センサ。
- マイクロ流体センサを組み立てて使用する方法であって、
基材上に導電材料を付着させて10ミクロン(μm)未満の厚さの少なくとも1つの電極を形成する段階と、
少なくとも1つの流路が形成されたスペーサ層の第1の表面を、該スペーサ層の前記少なくとも1つの流路が前記基材上の前記少なくとも1つの電極の上を通る状態で、前記少なくとも1つの電極を有する前記基板に取り付ける段階と、
カバー層を前記スペーサ層の第2の表面に取り付ける段階と、
を含む方法。 - 前記基材の上に前記導電材料を付着する段階は、前記導電材料をスクリーン印刷する段階を含む、請求項8に記載の方法。
- 前記基材の上に前記導電材料を付着する段階は、導電性インクを付着する段階を含む、請求項8に記載の方法。
- 前記基材の上に前記導電材料を付着する段階は、前記基材の上に送信電極及び受信電極を形成する段階を含む、請求項8、9、又は10に記載の方法。
- 前記流体が前記送信電極及び前記受信電極の上を流れることができるように、前記少なくとも1つの流路に流体を受け入れる段階と、
前記送信電極で所定の周波数及び電圧の交流を受信する段階と、
前記送信電極と前記受信電極との間のインピーダンスを測定する段階と、
を更に含む、請求項11に記載の方法。 - 前記インピーダンスを測定する段階は、所定期間のインピーダンスを測定する段階を含む、請求項12に記載の方法。
- 前記測定したインピーダンスに基づいて流体特性を計算する段階を更に含む、請求項12に記載の方法。
- 前記スペーサ層の前記第1の表面を前記基材に取り付ける段階は、前記少なくとも1つの流路が50μmから100μmの間の高さを有するように構成する段階を含む、請求項8から14のいずれか1項に記載の方法。
- 前記カバー層を前記スペーサ層の前記第2の表面へ取り付ける段階は、前記カバー層が、前記スペーサ層に形成された前記少なくとも1つの流路と界接する複数の貫通孔を有するように構成する段階を含む、請求項8から15のいずれか1項に記載の方法。
- 前記カバー層を前記スペーサ層の前記第2の表面へ取り付ける段階は、前記カバー層が前記流路内に配置された少なくとも1つの付着領域を有するように構成する段階と、少なくとも1つの付着領域に少なくとも1つの試薬を付着する段階と、を更に含む、請求項8から16のいずれか1項に記載の方法。
- 前記スペーサ層は、接着剤を使用して前記基材及び前記カバー層に取り付けられる、請求項8から17のいずれか1項に記載の方法。
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US14/083,347 | 2013-11-18 | ||
US14/083,347 US20150140671A1 (en) | 2013-11-18 | 2013-11-18 | Method and system for assembling a microfluidic sensor |
Publications (1)
Publication Number | Publication Date |
---|---|
JP2015108622A true JP2015108622A (ja) | 2015-06-11 |
Family
ID=53173683
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2014233308A Pending JP2015108622A (ja) | 2013-11-18 | 2014-11-18 | マイクロ流体センサ |
Country Status (4)
Country | Link |
---|---|
US (2) | US20150140671A1 (ja) |
JP (1) | JP2015108622A (ja) |
CN (1) | CN104655684A (ja) |
DE (1) | DE102014116777A1 (ja) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2020166545A1 (ja) | 2019-02-15 | 2020-08-20 | Phcホールディングス株式会社 | バイオセンサ |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105214744A (zh) * | 2015-10-26 | 2016-01-06 | 深圳华迈兴微医疗科技有限公司 | 一种磁微粒化学发光微流控芯片 |
CN105628747B (zh) * | 2015-12-18 | 2019-03-22 | 上海奥普生物医药有限公司 | 凝血时间测试分析装置及其测试分析方法 |
WO2017123266A1 (en) | 2016-01-16 | 2017-07-20 | Hewlett-Packard Development Company, L.P. | Blood characteristic measurement |
US20180321263A1 (en) * | 2016-01-16 | 2018-11-08 | Hewlett-Packard Development Company, L.P. | Blood characteristic measurement devices |
CN109100394B (zh) * | 2018-07-18 | 2020-10-02 | 浙江大学 | 一种凝血时间检测仪及其检测方法 |
US20210237058A1 (en) * | 2018-08-09 | 2021-08-05 | Hewlett-Packard Development Company, L.P. | Microfluidic devices to detect fluid priming |
CN109121283B (zh) * | 2018-08-15 | 2021-01-15 | 江门崇达电路技术有限公司 | 一种用于电化学传感器的印制电路板及其制作方法 |
TWI672500B (zh) * | 2018-11-02 | 2019-09-21 | 福永生物科技股份有限公司 | 電化學式感測試片及其製造方法 |
CN109682878A (zh) * | 2019-03-01 | 2019-04-26 | 南京岚煜生物科技有限公司 | 一种具有五层结构的多通道微流体凝血检测芯片 |
CN110715965B (zh) * | 2019-09-09 | 2020-08-18 | 浙江大学 | 一种双面生理数值测试片及其制备方法 |
Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6620310B1 (en) * | 2000-12-13 | 2003-09-16 | Lifescan, Inc. | Electrochemical coagulation assay and device |
JP2004132966A (ja) * | 2002-08-23 | 2004-04-30 | Lifescan Inc | 微細針と一体化した電気化学的検査細片およびこれに関連した方法 |
US20080297169A1 (en) * | 2007-05-31 | 2008-12-04 | Greenquist Alfred C | Particle Fraction Determination of A Sample |
US7674616B2 (en) * | 2006-09-14 | 2010-03-09 | Hemosense, Inc. | Device and method for measuring properties of a sample |
JP2010513931A (ja) * | 2006-12-22 | 2010-04-30 | アボツト・ダイアビーテイス・ケア・インコーポレイテツド | 検体センサおよび使用方法 |
JP2012524903A (ja) * | 2009-04-24 | 2012-10-18 | スリーエム イノベイティブ プロパティズ カンパニー | 電気化学的バイオセンサー用電極ストリップ及びその調製方法 |
JP2013099867A (ja) * | 2011-11-07 | 2013-05-23 | Sony Corp | 基板層の融着方法、マイクロ流体チップの製造方法及び基板層の融着装置 |
Family Cites Families (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB1299363A (en) * | 1968-09-27 | 1972-12-13 | Amiram Ur | Monitoring of chemical, bio-chemical and biological reactions, particularly blood-clotting |
GB0030929D0 (en) * | 2000-12-19 | 2001-01-31 | Inverness Medical Ltd | Analyte measurement |
US7291310B2 (en) * | 2002-12-17 | 2007-11-06 | The Regents Of The University Of Michigan | Microsystem for determining clotting time of blood and low-cost, single-use device for use therein |
GB0329220D0 (en) * | 2003-12-17 | 2004-01-21 | Inverness Medical Switzerland | System |
ATE490567T1 (de) * | 2004-03-08 | 2010-12-15 | Univ Illinois | Mikrofluidische elektrochemische reaktoren |
BRPI0607213B1 (pt) * | 2005-01-28 | 2017-04-04 | Univ Duke | aparelho para manipulação de gotículas em uma placa de circuito impresso |
US7520164B1 (en) * | 2006-05-05 | 2009-04-21 | E.I. Spectra, Llc | Thin film particle sensor |
US20080006530A1 (en) * | 2006-06-19 | 2008-01-10 | Handani Winarta | Capillary Flow Control in a Flow Channel |
US20080197018A1 (en) * | 2007-02-16 | 2008-08-21 | Jin Han | Device with a pre-defined and guided capillary fill design |
US20120122197A1 (en) * | 2010-11-12 | 2012-05-17 | Abner David Jospeh | Inkjet reagent deposition for biosensor manufacturing |
US20150191370A1 (en) * | 2012-05-29 | 2015-07-09 | Jmy Invest Aps | Device for treating a liquid |
KR101466222B1 (ko) * | 2012-06-01 | 2014-12-01 | 주식회사 아이센스 | 정확도가 향상된 전기화학적 바이오센서 |
US9291612B2 (en) * | 2012-07-16 | 2016-03-22 | Micropoint Bioscience, Inc. | Determining blood coagulation characteristics using residual accumulative conductive energy |
-
2013
- 2013-11-18 US US14/083,347 patent/US20150140671A1/en not_active Abandoned
-
2014
- 2014-11-17 CN CN201410657274.8A patent/CN104655684A/zh active Pending
- 2014-11-17 DE DE102014116777.5A patent/DE102014116777A1/de active Pending
- 2014-11-18 JP JP2014233308A patent/JP2015108622A/ja active Pending
-
2016
- 2016-11-07 US US15/344,985 patent/US20170052169A1/en not_active Abandoned
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6620310B1 (en) * | 2000-12-13 | 2003-09-16 | Lifescan, Inc. | Electrochemical coagulation assay and device |
JP2004132966A (ja) * | 2002-08-23 | 2004-04-30 | Lifescan Inc | 微細針と一体化した電気化学的検査細片およびこれに関連した方法 |
US7674616B2 (en) * | 2006-09-14 | 2010-03-09 | Hemosense, Inc. | Device and method for measuring properties of a sample |
JP2010513931A (ja) * | 2006-12-22 | 2010-04-30 | アボツト・ダイアビーテイス・ケア・インコーポレイテツド | 検体センサおよび使用方法 |
US20080297169A1 (en) * | 2007-05-31 | 2008-12-04 | Greenquist Alfred C | Particle Fraction Determination of A Sample |
JP2012524903A (ja) * | 2009-04-24 | 2012-10-18 | スリーエム イノベイティブ プロパティズ カンパニー | 電気化学的バイオセンサー用電極ストリップ及びその調製方法 |
JP2013099867A (ja) * | 2011-11-07 | 2013-05-23 | Sony Corp | 基板層の融着方法、マイクロ流体チップの製造方法及び基板層の融着装置 |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2020166545A1 (ja) | 2019-02-15 | 2020-08-20 | Phcホールディングス株式会社 | バイオセンサ |
Also Published As
Publication number | Publication date |
---|---|
US20150140671A1 (en) | 2015-05-21 |
DE102014116777A1 (de) | 2015-05-21 |
CN104655684A (zh) | 2015-05-27 |
US20170052169A1 (en) | 2017-02-23 |
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