JP2015077102A - Heating-cooling device and detection device - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a heating-cooling device capable of preventing the surface of a substrate from being contaminated while conducting a cooling performance.SOLUTION: A heating-cooling device 100 for heating and cooling a collection substrate 200 capable of collecting particles on a surface thereof includes a heater 2 for heating the collection substrate fixed inside a housing 1, and a fan 3 for generating an air flow by introducing outside air into the housing and evacuating the outside air, and thereby cooling the collection substrate fixed inside the housing. The heater can be mounted with the collection substrate on a top surface of the heater and functions as a fixing unit for fixing the collection substrate inside the housing so that the position of the collection substrate is fixed relative to the housing. An air inlet 1A and an exhaust port 1B are provided on a side of the housing facing a back surface of the fixed collection substrate. The fan is installed on the same side as the side to which the air inlet and exhaust port are provided relative to the fixed collection substrate.

Description

  The present invention relates to a heating / cooling device and a detection device, and more particularly to a heating / cooling device and a detection device for heating and cooling a substrate on which particles are collected.

  For example, when detecting particles derived from organisms such as microorganisms in the atmosphere, a method is adopted in which the particles are collected on the surface of the collection substrate using the inertial collision method and the particles derived from the surface are detected. . For this detection, for example, a technique disclosed in Japanese Patent Application Laid-Open No. 2008-187935 (hereinafter referred to as Patent Document 1) can be used. That is, it is possible to obtain a fluorescent image after pre-staining the collection substrate with a fluorescent staining reagent and discriminate biological particles from the area and luminance value of the fluorescent image. However, in this method, a staining operation with a fluorescent staining reagent is indispensable and complicated.

  As an alternative method, the applicant of the present application performs the above-described staining work by utilizing the fact that the fluorescence intensity from biological particles is increased by heating in JP 2013-520639 A (hereinafter referred to as Patent Document 2). We have proposed a method that can detect biologically-derived particles with high sensitivity and high accuracy without performing it.

JP 2008-187935 A Special table 2013-52039 gazette

  When a collection board | substrate is heated like patent document 2, a collection board | substrate is cooled for the next process after a heating. At that time, the collection substrate is usually cooled using a cooling fan or the like.

  However, when the airflow generated by the cooling fan directly hits the surface of the collection substrate, the particles collected on the surface when the detection target is a minute object such as a microorganism-derived particle as described above. There is a risk that the surface will be disturbed due to damage to the surface, or so-called “contamination” in the cooling process, and foreign matter may adhere to the surface. For example, when the collection substrate is used for detection as described above, there is a possibility that the detection accuracy is lowered due to the surface being disturbed by cooling of the collection substrate.

  The present invention has been made in view of such problems, and an object of the present invention is to provide a heating / cooling device capable of suppressing the disturbance of the substrate surface due to a cooling operation and a detection device including the heating / cooling device. .

  In order to achieve the above object, according to one aspect of the present invention, a heating / cooling device is a device for heating and cooling a collection substrate capable of collecting particles on a surface, and includes a housing, a housing, and a housing. On the other hand, a fixing part for fixing the position of the collection substrate, a heater for heating the collection substrate fixed in the housing, and generating airflow by introducing and exhausting outside air into the housing, And a fan for cooling the collection substrate fixed in the housing. An air supply port and an exhaust port are provided on the side of the housing facing the first surface of the collection substrate fixed by the fixing unit, and the fan supplies air to the collection substrate fixed by the fixing unit. It is installed on the same side as the side on which the opening and the exhaust port are provided.

  Preferably, an insertion port for inserting the collection substrate into the housing is further provided on the side of the housing facing the second surface of the back surface of the first surface of the collection substrate fixed by the fixing portion. The heating / cooling device further includes a lid portion for closing the insertion port so that resistance to outside air passing through the insertion port is larger than resistance to outside air passing through the air supply port.

  Preferably, the fixing portion fixes the collection substrate horizontally or substantially horizontally in the housing, and the side on which the first surface faces is the lower side of the collection substrate.

  According to another aspect of the present invention, the detection device is a device for detecting biologically-derived particles from the surface after heating and cooling a collection substrate capable of collecting particles on the surface. A fixed part for fixing the position of the collection substrate with respect to the body, a heater for heating the collection substrate fixed in the housing, and air flow is generated by introducing and exhausting outside air into the housing And a fan for cooling the collection substrate fixed in the housing. An air supply port and an exhaust port are provided on the side of the housing facing the first surface of the collection substrate fixed by the fixing unit, and the fan and the heater are connected to the collection substrate fixed by the fixing unit. It is installed on the same side as the side where the air supply and exhaust ports are provided. The detection device irradiates excitation light to the second surface, which is disposed on the side facing the second surface of the first surface of the collection substrate fixed by the fixing unit in the housing. And a detector including a light receiving element for receiving fluorescence from the light source and the second surface.

  Preferably, the detection apparatus is heated by a heater, and then the fluorescence intensity received by the light receiving element when excitation light is applied to the second surface of the collection substrate cooled by driving the fan. And a computing unit for detecting biologically derived particles from the second surface.

  According to the present invention, in the heating and cooling device, it is possible to suppress the disturbance of the substrate surface due to the cooling operation. Therefore, especially when the substrate after heating and cooling is used for detection, the detection accuracy can be improved.

It is the schematic which shows the specific example of a structure of the heating-cooling apparatus concerning 1st Embodiment. It is the schematic which shows the other specific example of a structure of the heating / cooling apparatus concerning 1st Embodiment. It is a graph showing the measurement result of transition of the surface temperature of a collection board. It is the schematic which shows the specific example of a structure of the heating-cooling apparatus concerning 2nd Embodiment. It is the schematic which shows the specific example of a structure of the heating-cooling apparatus concerning 2nd Embodiment. It is a figure showing the specific example of the external appearance of a drive part and a holding | maintenance part. It is a figure showing the specific example of the external appearance of a holding | maintenance part. It is a figure showing the other example of the drive part. It is the schematic which shows the specific example of a structure of the heating-cooling apparatus concerning 3rd Embodiment. It is the schematic which shows the specific example of a structure of the heating-cooling apparatus concerning 3rd Embodiment. It is the schematic which shows the other specific example of a structure of the heating-cooling apparatus concerning 3rd Embodiment. It is a figure showing the mode of the set of the collection board | substrate to the heating / cooling apparatus concerning 3rd Embodiment. It is the schematic which shows the specific example of a structure of the detection apparatus concerning 4th Embodiment. It is a measurement result of the fluorescence spectrum before and behind heat processing when Escherichia coli is heat-processed for 5 minutes at 200 degreeC. It is a fluorescence-microscope photograph before and behind heat processing when colon_bacillus | E._coli is heat-processed at 200 degreeC for 5 minute (s). It is a measurement result of the fluorescence spectrum before and behind heat processing when Bacillus bacteria are heat-processed at 200 degreeC for 5 minute (s). It is a fluorescence-microscope photograph before and behind heat processing when Bacillus bacteria are heat-processed for 5 minutes at 200 degreeC. It is a measurement result of the fluorescence spectrum before and behind heat processing when mold bacteria are heat-processed at 200 degreeC for 5 minute (s). It is a fluorescence-microscope photograph before and behind heat processing when mold bacteria are heat-processed at 200 degreeC for 5 minute (s). It is a measurement result of the fluorescence spectrum before and behind heat processing when the dust which emits fluorescence is heat-processed for 5 minutes at 200 degreeC. It is a fluorescence microscope photograph when the dust which emits fluorescence is heat-processed at 200 degreeC for 5 minute (s).

  Embodiments of the present invention will be described below with reference to the drawings. In the following description, the same parts and components are denoted by the same reference numerals. Their names and functions are also the same. Therefore, these descriptions will not be repeated.

  The heating / cooling device according to the present embodiment is a device that heats and then cools the collection substrate set (fixed) in the housing. The collection substrate to be heated and cooled may be a resin, glass, metal, silicon substrate, or a thin plate such as polydimethylsiloxane (PDMS) formed on a substrate such as silicon or resin. It may be a medium retained on a carrier such as a petri dish.

[First Embodiment]
FIG. 1 is a schematic diagram illustrating a specific example of the configuration of the heating and cooling device 100 according to the first embodiment. Referring to FIG. 1, heating / cooling device 100 includes a heater 2 and a cooling fan 3 arranged in housing 1.

  A ceramic heater is preferably used as the heater 2. In addition, a far infrared heater, a far infrared lamp, etc. may be sufficient. The heater 2 has a planar shape and is disposed in the housing 1 with the plane being horizontal or substantially horizontal. On this plane, the collection substrate 200 can be loaded with its surface in contact. Therefore, the heater 2 functions as a heating unit for heating the loaded collection substrate 200, and supports the collection substrate 200 on the plane, thereby allowing the collection substrate 200 to be placed in the housing 1. It also functions as a fixing part for fixing the position with respect to the housing 1.

  The fan 3 is driven to introduce outside air into the housing 1 and exhaust it to generate an air flow. It can be said that the fan 3 is an airflow generation mechanism for generating the above airflow in the housing 1. The airflow generation mechanism is a fan in this example, but is not limited to a fan and may be a pump or the like. The collection substrate 200 heated by the heater 2 can be cooled by contacting the collection substrate 200 to which the generated airflow is fixed. The housing 1 is provided with an air supply port 1A for introducing outside air into the housing 1 and an exhaust port 1B for exhausting air. The fan 3 is disposed in the housing 1 and between the air supply port 1A and the exhaust port 1B. Therefore, when the fan 3 is driven, an airflow is generated in the housing 1 from the air supply port 1A through the fan 3 to the exhaust port 1B.

  As shown in FIG. 1, the air supply port 1 </ b> A and the exhaust port 1 </ b> B of the housing 1 are on the side of the collection substrate 200 that is loaded and fixed on the upper surface of the heater 2 and is in contact with the upper surface of the heater 2. Provided on the side facing the surface. The fan 3 is also installed on the same side as the side on which the air supply port 1A and the exhaust port 1B are provided with respect to the collection substrate 200 described above. In the example of FIG. 1, the collection substrate 200 is loaded and fixed horizontally or substantially horizontally on the heater 2, the air supply port 1A is the side below the collection substrate 200 and the heater 2, and the exhaust port 1B is the heater. 2 is provided below. The fan 3 is disposed below the heater 2 and at a height between the air supply port 1A and the exhaust port 1B.

  In the example of FIG. 1 (and in the following description), the heater 2 and the fan 3 are arranged in the housing 1. However, these positions are not limited to the inside of the housing 1. That is, the heater 2 is disposed outside the housing 1, for example, the outside in contact with the housing 1, and indirectly heats the inside of the heating / cooling device 100 and the collection substrate 200 by heating the housing 1. May be. In this case, a support portion for fixing and supporting the position of the collection substrate 200 is provided in the housing 1 (as in the example described later).

  The position of the fan 3 is not limited to the position shown in FIG. In other words, the fan 3 is driven only on the side where the lower surface of the collection substrate 200 faces, that is, on the lower side of the collection substrate 200, the air flow generated from the air supply port 1 </ b> A to the exhaust port 1 </ b> B. It may be arranged at any position on the airflow. For example, when the air supply port 1A and the exhaust port 1B are provided at the positions shown in FIG. 1, the fan 3 is provided outside the housing 1 rather than the exhaust port 1B, and sucks the internal air from the exhaust port 1B. Then, it may be exhausted.

  As described above, the airflow generated by driving the fan 3 is used for cooling the collection substrate 200. Therefore, cooling efficiency can be improved by more air striking the lower surface of the collection substrate 200. Therefore, as shown in FIG. 2, a guide 1A ′ for guiding outside air introduced from the air supply port 1A to the lower surface of the collection substrate 200 is preferably provided in the vicinity of the air supply port 1A. Is provided. Thereby, cooling efficiency can be improved.

  The heating / cooling device 100 further includes a control device 10 having a CPU (Central Processing Unit) 10A. The control device 10 is electrically connected to the heater 2 and the fan 3 and controls their drive. That is, the control device 10 causes the heater 2 to generate heat with a predetermined amount of heat for a predetermined time. Thereafter, the control device 10 rotates the fan 3 at a predetermined rotational speed for a predetermined time. The control device 10 corresponds to, for example, a general PC (personal computer) connected to a power source for driving the heater 2 and the like so that the voltage value and output ON / OFF can be controlled. Preferably, the heating / cooling device 100 further includes a thermocouple for measuring the surface temperature of the collection substrate 200 (not shown), and a signal from the thermocouple is input to the control device 10. The control device 10 may perform feedback control of the heater 2 so that the surface of the collection substrate 200 becomes a predetermined temperature (for example, 200 ° C. during heating) by collecting signals from the thermocouple.

  FIG. 3 is a graph showing the measurement result of the transition of the surface temperature of the collection substrate 200. This represents a measurement result of the surface temperature of the collection substrate 200 when the inventor heated and cooled the collection substrate 200 using the heating and cooling device 100 according to the first embodiment. Referring to FIG. 3, the control device 10 starts heating by the heater 2 and performs the feedback control described above, so that the surface temperature of the collection substrate 200 is a target temperature of 200 ° C. necessary for heating the microorganisms. It was confirmed that it was heated up to. It was also confirmed that when the control device 10 turns off the heater 2 and starts driving the fan 3, the collection substrate 200 is cooled and the surface temperature rapidly decreases. Note that no foreign matter was adhered to the surface of the collection substrate 200 by driving the fan 3.

<Effect of the first embodiment>
By arranging the fan 3, the air supply port 1A, and the exhaust port 1B as described above, the fan 3 is driven to drive the housing 1 into the housing 1 as represented by the curve with an arrow in FIG. The generated airflow from the air supply port 1 </ b> A to the exhaust port 1 </ b> B through the fan 3 is generated only below the collection substrate 200 loaded and fixed on the upper surface of the heater 2.

  When the collection substrate 200 collecting particles on the collection surface (also referred to as the surface) is loaded on the upper surface of the heater 2, the heater is placed so that the surface does not fall and the back surface is in contact with the upper surface of the heater 2. 2 It will be loaded on the upper surface. Therefore, the fan 3 and the air intake port 1 </ b> A and the exhaust port 1 </ b> B are set on the side facing the back surface of the collection substrate 200, that is, below the collection substrate 200, so It occurs only on the back side. For this reason, it is possible to prevent particles on the surface of the collection substrate 200 from being damaged by the air flow, or so-called “contamination” from adhering to the surface of the collection substrate 200.

[Second Embodiment]
4 and 5 are schematic views showing a specific example of the configuration of the heating and cooling device 100 according to the second embodiment. 4 and 5, the heating / cooling device 100 according to the second embodiment is similar to the heating / cooling device 100 according to the first embodiment. And a cooling fan 3. The heater 2 and the fan 3 are the same as those included in the heating / cooling device 100 according to the first embodiment.

  The heating / cooling device 100 according to the second embodiment is a drive mechanism for moving the holding substrate 4 for holding the collection substrate 200 and the collection substrate 200 held by the holding unit 4. The driving unit 5 is further included. The drive unit 5 is electrically connected to the control device 10 and its drive is controlled. That is, the drive unit 5 controls the collection substrate 200 held by the holding unit 4 under the control of the control device 10 at the first position (FIG. 4) where the back surface is in contact with the top surface of the heater 2 and the back surface. Is moved between a second position (FIG. 5) which is a position away from the upper surface of the heater 2.

  FIG. 6 is a diagram illustrating a specific example of the appearance of the drive unit 5 and the holding unit 4. Referring to FIG. 6, as an example, drive unit 5 has an arm extending horizontally or substantially horizontally, and supports holding unit 4 with the arm. The drive unit 5 is configured to be movable in a horizontal direction that is a support direction of the holding unit 4 and a vertical direction that is a direction orthogonal to the support direction of the holding unit 4.

  FIG. 7 is a diagram illustrating a specific example of the appearance of the holding unit 4. With reference to FIG. 7, the holding unit 4 has a surface for holding the collection substrate 200 as an example, and the collection substrate 200 is mounted on the surface, or fixed with screws or the like, The collection substrate 200 is held.

  FIG. 8 is a diagram illustrating another example of the drive unit 5. Referring to FIG. 8, drive unit 5 is not limited to being movable only in the two-axis direction shown in FIG. 6, and may be movable in the three-axis direction as shown in FIG. 8. Good.

  The controller 10 controls the drive unit 5 when heating the collection substrate 200 to set the collection substrate 200 to the first position (FIG. 4), and after heating, the collection substrate 200 is moved to the second position. (Fig. 5).

<Effects of Second Embodiment>
Heating efficiency and cooling efficiency can be increased by the above-described control of the drive unit 5 by the control device 10. In addition, since the collection substrate 200 can be easily attached to and detached from the heater 2, the operation of replacing the collection substrate 200 becomes easy and the running cost for replacement can be suppressed. Moreover, the accuracy of the position of attachment / detachment with respect to the heater 2 can be improved.

[Third Embodiment]
FIG. 9 and FIG. 10 are schematic views showing a specific example of the configuration of the heating and cooling device 100 according to the third embodiment. Referring to FIGS. 9 and 10, the heating / cooling device 100 according to the third embodiment is also similar to the heating / cooling device 100 according to the first embodiment, and the heater 2 disposed in the housing 1. And a cooling fan 3. The heater 2 and the fan 3 are the same as those included in the heating / cooling device 100 according to the first embodiment. Moreover, the heating / cooling device 100 according to the third embodiment is held by the holding unit 4 for holding the collection substrate 200 and the holding unit 4, similarly to the heating / cooling device 100 according to the second embodiment. It further includes a drive unit 5 that is a drive mechanism for moving the collection substrate 200 in the formed state. The holding unit 4 and the driving unit 5 are the same as those described with reference to FIGS.

  The casing 1 of the heating and cooling apparatus 100 according to the third embodiment is further provided with an introduction port 1C. The drive unit 5 has a first position where the back surface is in contact with the upper surface of the heater 2 in the housing 1 and a second position outside the housing 1 for the collection substrate 200 held by the holding unit 4. Between the first and second casings 1 through the inlet 1C of the casing 1. As an example, as illustrated in FIGS. 6 and 8, the driving unit 5 supports the collection substrate 200 held by the holding unit 4 while supporting the holding unit 4 holding the collection substrate 200 by an arm. It moves between said 1st position and 2nd position via the inlet 1C.

  In order to achieve uniform and efficient heating of the collection substrate 200 using the heater 2, it is ensured that at least the particle collection position of the collection substrate 200, preferably the entire back surface is the upper surface of the heater. It is preferable to contact. Therefore, preferably, as shown in FIG. 11, the heating / cooling device 100 according to the third embodiment is a surface opposite to the lower surface of the heater 2, that is, the upper surface in contact with the collection substrate 200. The shock absorbing member 21 arranged at the position is included. The buffer member 21 corresponds to a spring such as a leaf spring or a spring, or a resin such as rubber or sponge.

  When the drive unit 5 brings the collection substrate 200 held by the holding unit 4 under the control of the control device 10 into contact with the upper surface of the heater 2, the drive unit 5 gives the collection substrate 200 a force enough to deform the buffer member 21. Add it downward. As a result, an upward force is generated from the buffer member 21 toward the heater 2, and the collection substrate 200 and the heater 2 come into close contact with each other.

  When the buffer member 21 is not provided, for example, when the back surface of the collection substrate 200 is inserted with an inclination with respect to the top surface of the heater 2, the contact area between the back surface of the collection substrate 200 and the top surface of the heater 2 is small. Become. Therefore, uniform and efficient heating is difficult. In this case, if the buffer member 21 is provided, an upward force is generated from the buffer member 21 toward the heater 2 even if there is an inclination as described above, whereby the collection substrate 200 and the heater 2 are provided. Makes it possible to achieve uniform and efficient heating. Therefore, the detection accuracy of biological particles can be improved.

  Referring to FIG. 7, preferably, the drive unit 5 has a resistance (fluid resistance) to air passing through the inlet 1 </ b> C of the housing 1 when the collection substrate 200 is in the first position. Is provided with a fixture 61 as an example of a lid for increasing the resistance to the air passing through the air supply port 1A. The fixing tool 61 covers a portion other than the arm of the introduction port 1 </ b> C in a state where the arm of the drive unit 5 exists in the introduction port 1 </ b> C when the collection substrate 200 reaches the first position. That is, the fixture 61 has a surface wider than the introduction port 1C. As an example, the fixture 61 is provided at the end of the holding unit 4 opposite to the side inserted into the heating / cooling device 100, and the collection substrate 200 held by the holding unit 4 is attached to the first in the housing 1. When inserted into the housing 1 for the 1 position, the fixture 61 covers the inlet 1C. More preferably, an O-ring 62 is installed as an example of a member for increasing resistance to air at a position where the fixture 61 is in contact with the introduction port 1C. As an example of a member for increasing resistance to air, the O-ring 62 may be provided on the introduction port 1C side.

  FIG. 12 is a diagram illustrating how the collection substrate 200 is set in the heating / cooling device 100 according to the third embodiment. With reference to FIG. 12, the drive unit 5 introduces the collection substrate 200 held by the holding unit 4 into the housing 1 through the introduction port 1 </ b> C. When the collection substrate 200 is positioned on the heater 2, the drive unit 5 presses the collection substrate 200 downward against the heater 2 in accordance with a control signal from the control device 10, and moves the fixture 61 to the inlet 1 </ b> C for a specified time. The pressure is maintained in the direction of pressing against. Thereby, the fluid resistance with respect to the air which passes 1 C of inlets can be raised.

<Effect of the third embodiment>
When the heating / cooling device 100 has the above configuration, the setting of the collection substrate 200 from the collection device or the like into the housing 1 of the heating / cooling device 100 can be automated, and the operation becomes easy. In addition, the accuracy of the set position can be improved.

  In that case, the structure which covers 1 C of inlets with the fixing tool 61 which is a cover part can suppress significantly the air which passes 1 C of inlets at the time of cooling compared with the quantity of the air which passes 1 A of air inlets. . Therefore, even if the inlet 1 </ b> C is provided on the surface side of the collection substrate 200, the airflow generated on the surface side of the collection substrate 200 during cooling can be suppressed. Thereby, it is possible to prevent the particles on the surface of the collection substrate 200 from being damaged by the air flow, or the so-called “contamination” from adhering to the surface of the collection substrate 200.

[Fourth Embodiment]
The detection device according to the fourth embodiment includes any one of the heating and cooling devices according to the first to third embodiments, and is captured on the surface after heating and cooling of the collection substrate. Detecting particles in the collected air.

  FIG. 13 is a schematic diagram illustrating a specific example of the configuration of a detection apparatus 100 ′ according to the fourth embodiment. Referring to FIG. 13, detection device 100 ′ includes, as an example, heating / cooling device 100 according to the first embodiment, and detection unit 300 for detecting particles collected on the surface of collection substrate 200. including. That is, the detection apparatus 100 ′ is configured such that the heating / cooling apparatus 100 further includes the detection unit 300.

  The detection method in the detection unit 300 is not limited to a specific method, and a method of detecting scattered light derived from particles, a method of detecting fluorescence derived from particles, or acquiring a particle image and performing the image recognition processing. Detection by the above can be suitably employed.

  The detection unit 300 includes a light source 31 for irradiating the surface of the collection substrate 200 and a light receiving element 32 for receiving light from the surface of the collection substrate 200. The light receiving element 32 is electrically connected to the control device 10 and inputs a detection signal indicating the amount of received light to the control device 10.

  The control device 10 causes the light source 31 to emit light after being heated by the heater 2 and cooled by the fan 3, and calculates the amount of biological particles based on the amount of light received from the light receiving element 32 in that state. The control device 10 may calculate the amount of biological particles based on the amount of light received from the light receiving element 32 before and after heating and cooling.

  In the case of the method for detecting scattered light, the light receiving element 32 corresponds to a photodiode or a photomultiplier tube for measuring scattered light from particles on the surface of the collection substrate 200. The light receiving element 32 may be arranged at any angle with respect to the irradiation direction of the light source 31. The light receiving element 32 detects forward scatter, side scatter, back scatter, and the like according to the arranged position. The control device 10 that has received the detection signal from the light receiving element 32 calculates the particle diameter, the complexity of the configuration within the particle, and the like, and compares it with a reference value that is stored in advance, so that the target particle (for example, biological origin) is obtained. Particles). Or the control apparatus 10 should just count the signal pulse of scattered light, when confirming the presence or absence and number of particle | grains.

  In the case of a method for detecting fluorescence, the light source 31 emits excitation light. The light receiving element 32 corresponds to an image sensor such as a photodiode, a photomultiplier tube, a CCD (Charge Coupled Device) image sensor, or a CMOS (Complementary Metal Oxide Semiconductor) image sensor for measuring fluorescence from particles. The detection unit 300 may be provided with a fluorescence detection lens and a fluorescence detection filter such as a band pass filter and a long pass filter as necessary. Further, a light source lens for adjusting the irradiation direction may be disposed in front of the irradiation direction of the light source 31. Depending on the angle at which the light receiving element is disposed, a mirror for adjusting the optical path such as a dichroic mirror may be provided.

  When performing image recognition processing, the light receiving element 32 corresponds to an image sensor such as a CCD image sensor or a CMOS image sensor for measuring (photographing) a particle image on the surface of the collection substrate 200. The control device 10 that has received the particle image from the light receiving element 32 detects the target particle (for example, biological particles) and the presence / absence of the particle by comparing with a previously stored particle image.

<Detection principle>
Some non-living particles, such as chemical fiber dust, floating in the air emit fluorescence when irradiated with ultraviolet light or blue light, similar to biological particles. However, the fluorescence intensity of biological particles increases by heating, whereas the fluorescence intensity of non-biological particles such as chemical fiber dust does not change by heating. The detection apparatus 100 ′ according to the fourth embodiment uses this property to detect biological particles in the air. Hereinafter, this principle will be described in detail.

  FIG. 14 shows measurement results of fluorescence spectra before and after the heat treatment (curve 71) when Escherichia coli is heat treated at 200 ° C. for 5 minutes as biological particles. FIG. 15A is a fluorescence micrograph before heat treatment, and FIG. 15B is a fluorescence micrograph after heat treatment. From the measurement results shown in FIG. 14 and the comparison between (A) and (B) in FIG. 15, it can be seen that the fluorescence intensity from E. coli is greatly increased by the heat treatment.

  Similarly, FIG. 16 is a measurement result of fluorescence spectra before and after heat treatment (curve 73) when Bacillus bacteria are heat-treated at 200 ° C. for 5 minutes as biological particles. . FIG. 17A is a fluorescence micrograph before heat treatment, and FIG. 17B is a fluorescence micrograph after heat treatment. From the measurement results shown in FIG. 16 and the comparison between (A) and (B) in FIG. 17, it can be seen that the fluorescence intensity from Bacillus bacteria is greatly increased by the heat treatment.

  Similarly, FIG. 18 is a measurement result of fluorescence spectra before and after the heat treatment (curve 76) when mold fungi are heat-treated at 200 ° C. for 5 minutes as biological particles. . FIG. 19A is a fluorescence micrograph before heat treatment, and FIG. 19B is a fluorescence micrograph after heat treatment. From the measurement results shown in FIG. 18 and the comparison between (A) and (B) in FIG. 19, it can be seen that the fluorescence intensity from the mold is greatly increased by the heat treatment.

  On the other hand, FIG. 20A and FIG. 20B respectively show before the heat treatment (curve 77) and after the heat treatment (curve 78) when the fluorescent dust is heat-treated at 200 ° C. for 5 minutes. ) Of the fluorescence spectrum. FIG. 21A is a fluorescence micrograph before heat treatment, and FIG. 21B is a fluorescence micrograph after heat treatment. When FIG. 20A and FIG. 20B are compared, these fluorescence spectra almost overlap. That is, it can be seen from the comparison between FIGS. 20A and 20B and the comparison between FIGS. 21A and 21B that the fluorescence intensity from dust does not change before and after the heat treatment.

  Therefore, the control apparatus 10 measures the particle | grains on the surface of the collection board | substrate 200 after heating with the detection part 300, after heating the collection board | substrate 200 which collected the particle | grains with the heater 2 for a predetermined time and cooling with the fan 3 ( Take a particle image). Thereby, measurement results as shown in FIGS. 15B, 17B, 19B, and 21B can be obtained. The control device 10 detects particles whose fluorescence intensity from the collection substrate 200 after heating and cooling is equal to or higher than a threshold value stored in advance as biological particles.

  As another method, the control device 10 measures the particles on the surface of the collection substrate 200 before heating by the detection unit 300 without heating and cooling the collection substrate 200 that collected the particles (takes a particle image). Then, after heating and cooling the collection substrate 200, the detection unit 300 may measure the particles on the surface of the collection substrate 200 after heating (take a particle image). Thereby, measurement results as shown in FIGS. 15A and 15B, FIGS. 17A and 17B, FIGS. 19A and 19B, and FIGS. 21A and 21B are obtained. be able to. And the control apparatus 10 detects the particle | grains more than the threshold value which previously memorize | stored the difference ((B)-(A)) of the fluorescence intensity from the collection board | substrate 200 before and behind a heating as a biological particle. May be.

<Effect of the fourth embodiment>
Since the detection apparatus 100 ′ according to the fourth embodiment has the above configuration, a series of operations from heating and cooling to detection can be automatically performed by one apparatus. Thereby, it is possible to detect biologically derived particles from particles collected on the surface of the collection substrate 200 with higher speed and higher accuracy.

  Furthermore, the detection apparatus 100 ′ uses the fluorescence intensity after heating and cooling (or the difference between the fluorescence intensity before and after heating and cooling) to convert the biological particles into non-biological particles without requiring treatment with a fluorescent staining reagent. And can be detected with high accuracy.

  In particular, the detection apparatus 100 ′ can perform heating / cooling and detection without disturbing the particles collected on the surface of the collection substrate 200 by a predetermined collection operation, so that it can detect biologically derived particles with high accuracy. It becomes possible to do.

<Other examples>
In the above description, an example is given in which the collection substrate 200 after being heated and cooled by the heating and cooling device 100 is used for detection of biological particles. However, the collection substrate 200 is not limited to that used for the detection operation. Therefore, the heating / cooling device 100 can be used for any device as long as the particles collected on the substrate surface are used after heating and cooling. For example, the heating / cooling device 100 can be used to heat and cool a substrate for use in an operation of amplifying a gene collected on the substrate surface (for example, polymerase chain reaction (PCR)).

  The embodiment disclosed this time should be considered as illustrative in all points and not restrictive. The scope of the present invention is defined by the terms of the claims, rather than the description above, and is intended to include any modifications within the scope and meaning equivalent to the terms of the claims.

  DESCRIPTION OF SYMBOLS 1 Housing, 1A air supply port, 1A 'guide, 1B exhaust port, 1C introduction port, 2 heater, 3 fan, 4 holding | maintenance part, 5 drive part, 10 control apparatus, 21 buffer member, 31 light source, 32 light receiving element, 61 Fixing tool, 62 O-ring, 71-78 curve, 100 heating / cooling device, 100 ′ detection device, 200 collection substrate, 300 detection unit.

Claims (5)

An apparatus for heating and cooling a collection substrate capable of collecting particles on a surface,
In the housing, a fixing portion for fixing the position of the collection substrate with respect to the housing,
A heater for heating the collection substrate fixed in the housing;
A fan for cooling the collection substrate fixed in the housing by generating an air flow by introducing and exhausting outside air into the housing;
An air supply port and an exhaust port are provided on the side of the housing facing the first surface of the collection substrate fixed by the fixing unit,
The heating and cooling device, wherein the fan is installed on the same side as the side on which the air supply port and the exhaust port are provided with respect to the collection substrate fixed by the fixing unit. An insertion port for inserting the collection substrate into the housing is provided on the side of the housing facing the second surface of the back surface of the first surface of the collection substrate fixed by the fixing portion. Further provided,
The heating / cooling device according to claim 1, further comprising a lid for closing the insertion port so that resistance to outside air passing through the insertion port is larger than resistance to outside air passing through the air supply port. . The fixing portion fixes the collection substrate horizontally or substantially horizontally in the housing,
The heating / cooling device according to claim 1 or 2, wherein a side to which the first surface faces is a lower side of the collection substrate. An apparatus for detecting biologically derived particles from the surface after heating and cooling a collection substrate capable of collecting particles on the surface,
In the housing, a fixing portion for fixing the position of the collection substrate with respect to the housing,
A heater for heating the collection substrate fixed in the housing;
A fan for cooling the collection substrate fixed in the housing by generating an air flow by introducing and exhausting outside air into the housing;
An air supply port and an exhaust port are provided on the side of the housing facing the first surface of the collection substrate fixed by the fixing unit,
The fan and the heater are installed on the same side as the side on which the air supply port and the exhaust port are provided with respect to the collection substrate fixed by the fixing unit,
Irradiating excitation light to the second surface of the housing, which is disposed on the side facing the second surface of the back surface of the first surface of the collection substrate fixed by the fixing portion. A detection device further comprising a detection unit including a light source for receiving light and a light receiving element for receiving fluorescence from the second surface.   The fluorescence intensity received by the light receiving element when the excitation light is applied to the second surface of the collection substrate heated by the heater and then cooled by driving the fan. The detection device according to claim 4, further comprising a calculation unit for detecting the biological particle from the second surface.