JP2014526884A5 - - Google Patents
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- JP2014526884A5 JP2014526884A5 JP2014517838A JP2014517838A JP2014526884A5 JP 2014526884 A5 JP2014526884 A5 JP 2014526884A5 JP 2014517838 A JP2014517838 A JP 2014517838A JP 2014517838 A JP2014517838 A JP 2014517838A JP 2014526884 A5 JP2014526884 A5 JP 2014526884A5
- Authority
- JP
- Japan
- Prior art keywords
- antibody
- amino acid
- heavy chain
- charged amino
- terminal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 210000004027 cell Anatomy 0.000 claims description 67
- 125000000539 amino acid group Chemical group 0.000 claims description 58
- 210000004899 c-terminal region Anatomy 0.000 claims description 54
- 238000000034 method Methods 0.000 claims description 47
- 235000001014 amino acid Nutrition 0.000 claims description 25
- 125000003588 lysine group Chemical group [H]N([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 claims description 24
- 150000001413 amino acids Chemical class 0.000 claims description 23
- 239000002773 nucleotide Substances 0.000 claims description 22
- 125000003729 nucleotide group Chemical group 0.000 claims description 22
- 239000000203 mixture Substances 0.000 claims description 14
- 230000004048 modification Effects 0.000 claims description 11
- 238000012986 modification Methods 0.000 claims description 11
- 125000001433 C-terminal amino-acid group Chemical group 0.000 claims description 10
- 108091005804 Peptidases Proteins 0.000 claims description 10
- 239000004365 Protease Substances 0.000 claims description 10
- 125000001500 prolyl group Chemical group [H]N1C([H])(C(=O)[*])C([H])([H])C([H])([H])C1([H])[H] 0.000 claims description 10
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims description 8
- 239000004472 Lysine Substances 0.000 claims description 8
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 8
- 238000004113 cell culture Methods 0.000 claims description 8
- 108020004705 Codon Proteins 0.000 claims description 6
- 230000004540 complement-dependent cytotoxicity Effects 0.000 claims description 6
- 239000003814 drug Substances 0.000 claims description 6
- 102000005367 Carboxypeptidases Human genes 0.000 claims description 4
- 108010006303 Carboxypeptidases Proteins 0.000 claims description 4
- 240000008042 Zea mays Species 0.000 claims description 4
- 238000012258 culturing Methods 0.000 claims description 4
- 210000004408 hybridoma Anatomy 0.000 claims description 4
- 235000018102 proteins Nutrition 0.000 claims description 4
- 102000004169 proteins and genes Human genes 0.000 claims description 4
- 108090000623 proteins and genes Proteins 0.000 claims description 4
- 230000017854 proteolysis Effects 0.000 claims description 4
- 238000006467 substitution reaction Methods 0.000 claims description 4
- 230000003993 interaction Effects 0.000 claims description 3
- 230000002797 proteolythic effect Effects 0.000 claims description 3
- 241000219194 Arabidopsis Species 0.000 claims description 2
- 239000004475 Arginine Chemical group 0.000 claims description 2
- 241000228212 Aspergillus Species 0.000 claims description 2
- 241001513093 Aspergillus awamori Species 0.000 claims description 2
- 241000228245 Aspergillus niger Species 0.000 claims description 2
- 240000002791 Brassica napus Species 0.000 claims description 2
- 235000011293 Brassica napus Nutrition 0.000 claims description 2
- 244000000626 Daucus carota Species 0.000 claims description 2
- 235000002767 Daucus carota Nutrition 0.000 claims description 2
- 241000196324 Embryophyta Species 0.000 claims description 2
- 244000068988 Glycine max Species 0.000 claims description 2
- 235000010469 Glycine max Nutrition 0.000 claims description 2
- 102000003886 Glycoproteins Human genes 0.000 claims description 2
- 108090000288 Glycoproteins Proteins 0.000 claims description 2
- 241000235058 Komagataella pastoris Species 0.000 claims description 2
- 244000207740 Lemna minor Species 0.000 claims description 2
- 235000006439 Lemna minor Nutrition 0.000 claims description 2
- 241000124008 Mammalia Species 0.000 claims description 2
- 206010028980 Neoplasm Diseases 0.000 claims description 2
- 241000208125 Nicotiana Species 0.000 claims description 2
- 241000207746 Nicotiana benthamiana Species 0.000 claims description 2
- 235000002637 Nicotiana tabacum Nutrition 0.000 claims description 2
- 241000320412 Ogataea angusta Species 0.000 claims description 2
- 241001489174 Ogataea minuta Species 0.000 claims description 2
- 240000007594 Oryza sativa Species 0.000 claims description 2
- 235000007164 Oryza sativa Nutrition 0.000 claims description 2
- 102000001708 Protein Isoforms Human genes 0.000 claims description 2
- 108010029485 Protein Isoforms Proteins 0.000 claims description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 2
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 2
- 241000223259 Trichoderma Species 0.000 claims description 2
- 235000007244 Zea mays Nutrition 0.000 claims description 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 2
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 2
- 230000006229 amino acid addition Effects 0.000 claims description 2
- 210000000628 antibody-producing cell Anatomy 0.000 claims description 2
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Chemical group OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims description 2
- 125000000637 arginyl group Chemical group N[C@@H](CCCNC(N)=N)C(=O)* 0.000 claims description 2
- 201000011510 cancer Diseases 0.000 claims description 2
- 230000022534 cell killing Effects 0.000 claims description 2
- 230000006037 cell lysis Effects 0.000 claims description 2
- 210000004978 chinese hamster ovary cell Anatomy 0.000 claims description 2
- 238000003776 cleavage reaction Methods 0.000 claims description 2
- 235000005822 corn Nutrition 0.000 claims description 2
- 238000012217 deletion Methods 0.000 claims description 2
- 230000037430 deletion Effects 0.000 claims description 2
- 230000000694 effects Effects 0.000 claims description 2
- 230000002708 enhancing effect Effects 0.000 claims description 2
- 230000002255 enzymatic effect Effects 0.000 claims description 2
- 230000002538 fungal effect Effects 0.000 claims description 2
- 125000000291 glutamic acid group Chemical group N[C@@H](CCC(O)=O)C(=O)* 0.000 claims description 2
- 230000013595 glycosylation Effects 0.000 claims description 2
- 238000006206 glycosylation reaction Methods 0.000 claims description 2
- 210000005260 human cell Anatomy 0.000 claims description 2
- 230000037361 pathway Effects 0.000 claims description 2
- 235000009566 rice Nutrition 0.000 claims description 2
- 230000007017 scission Effects 0.000 claims description 2
- 230000003248 secreting effect Effects 0.000 claims description 2
- 238000011191 terminal modification Methods 0.000 claims description 2
- 210000005253 yeast cell Anatomy 0.000 claims description 2
- 102000035195 Peptidases Human genes 0.000 claims 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000010998 test method Methods 0.000 description 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161504987P | 2011-07-06 | 2011-07-06 | |
| DKPA201100518 | 2011-07-06 | ||
| US61/504,987 | 2011-07-06 | ||
| DKPA201100518 | 2011-07-06 | ||
| PCT/EP2012/063338 WO2013004841A1 (en) | 2011-07-06 | 2012-07-06 | Modulation of complement-dependent cytotoxicity through modifications of the c-terminus of antibody heavy chains |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018153058A Division JP6718488B2 (ja) | 2011-07-06 | 2018-08-16 | 抗体重鎖のｃ末端の修飾による補体依存性細胞傷害の調節 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2014526884A JP2014526884A (ja) | 2014-10-09 |
| JP2014526884A5 true JP2014526884A5 (https=) | 2015-08-27 |
| JP6454547B2 JP6454547B2 (ja) | 2019-01-16 |
Family
ID=47436553
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014517838A Active JP6454547B2 (ja) | 2011-07-06 | 2012-07-06 | 抗体重鎖のｃ末端の修飾による補体依存性細胞傷害の調節 |
| JP2018153058A Active JP6718488B2 (ja) | 2011-07-06 | 2018-08-16 | 抗体重鎖のｃ末端の修飾による補体依存性細胞傷害の調節 |
| JP2022128990A Active JP7655886B2 (ja) | 2011-07-06 | 2022-08-12 | 抗体重鎖のｃ末端の修飾による補体依存性細胞傷害の調節 |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018153058A Active JP6718488B2 (ja) | 2011-07-06 | 2018-08-16 | 抗体重鎖のｃ末端の修飾による補体依存性細胞傷害の調節 |
| JP2022128990A Active JP7655886B2 (ja) | 2011-07-06 | 2022-08-12 | 抗体重鎖のｃ末端の修飾による補体依存性細胞傷害の調節 |
Country Status (4)
| Country | Link |
|---|---|
| US (2) | US10323081B2 (https=) |
| EP (1) | EP2729496B8 (https=) |
| JP (3) | JP6454547B2 (https=) |
| WO (1) | WO2013004841A1 (https=) |
Families Citing this family (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2702077A2 (en) | 2011-04-27 | 2014-03-05 | AbbVie Inc. | Methods for controlling the galactosylation profile of recombinantly-expressed proteins |
| EP2729496B8 (en) | 2011-07-06 | 2017-10-18 | Genmab A/S | Modulation of complement-dependent cytotoxicity through modifications of the c-terminus of antibody heavy chains |
| US9067990B2 (en) | 2013-03-14 | 2015-06-30 | Abbvie, Inc. | Protein purification using displacement chromatography |
| US9150645B2 (en) | 2012-04-20 | 2015-10-06 | Abbvie, Inc. | Cell culture methods to reduce acidic species |
| WO2013158273A1 (en) | 2012-04-20 | 2013-10-24 | Abbvie Inc. | Methods to modulate c-terminal lysine variant distribution |
| CN110563850A (zh) | 2012-04-30 | 2019-12-13 | 比奥孔有限公司 | 靶向/免疫调节性融合蛋白及其制造方法 |
| US9249182B2 (en) | 2012-05-24 | 2016-02-02 | Abbvie, Inc. | Purification of antibodies using hydrophobic interaction chromatography |
| US9512214B2 (en) | 2012-09-02 | 2016-12-06 | Abbvie, Inc. | Methods to control protein heterogeneity |
| KR20200024345A (ko) | 2013-01-10 | 2020-03-06 | 젠맵 비. 브이 | 인간 IgG1 Fc 영역 변이체 및 그의 용도 |
| CA3081073C (en) * | 2013-03-12 | 2023-09-12 | Biocon Ltd. | Fusion immunomodulatory proteins and methods for making same |
| SG11201507230PA (en) | 2013-03-12 | 2015-10-29 | Abbvie Inc | Human antibodies that bind human tnf-alpha and methods of preparing the same |
| US9017687B1 (en) | 2013-10-18 | 2015-04-28 | Abbvie, Inc. | Low acidic species compositions and methods for producing and using the same using displacement chromatography |
| WO2014151878A2 (en) | 2013-03-14 | 2014-09-25 | Abbvie Inc. | Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosacharides |
| FR3003171B1 (fr) * | 2013-03-15 | 2015-04-10 | Lab Francais Du Fractionnement | Nouveaux medicaments comprenant une composition d'anticorps enrichie en isoforme de charge majoritaire |
| US9598667B2 (en) | 2013-10-04 | 2017-03-21 | Abbvie Inc. | Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins |
| US9181337B2 (en) | 2013-10-18 | 2015-11-10 | Abbvie, Inc. | Modulated lysine variant species compositions and methods for producing and using the same |
| US9085618B2 (en) | 2013-10-18 | 2015-07-21 | Abbvie, Inc. | Low acidic species compositions and methods for producing and using the same |
| US20150139988A1 (en) | 2013-11-15 | 2015-05-21 | Abbvie, Inc. | Glycoengineered binding protein compositions |
| EP3699269A1 (en) * | 2015-09-22 | 2020-08-26 | F. Hoffmann-La Roche AG | Expression of fc-containing proteins |
| EP3774879B1 (en) * | 2018-03-30 | 2026-01-28 | Amgen Inc. | C-terminal antibody variants |
| MA53122A (fr) * | 2018-07-13 | 2021-05-19 | Genmab As | Variants d'anticorps cd38 et leurs utilisations |
| EP3929214A4 (en) * | 2019-05-30 | 2022-06-22 | Shandong Boan Biotechnology Co., Ltd. | ANTIBODY OR CHIMERIC ANTIGEN RECEPTOR DIRECTED AGAINST CLAUDIN 18.2 |
| TWI869528B (zh) | 2020-01-13 | 2025-01-11 | 美商威特拉公司 | C5ar1抗體分子及其用途 |
| WO2021227937A1 (zh) * | 2020-05-11 | 2021-11-18 | 神州细胞工程有限公司 | 通过与改变的Fc片段形成融合蛋白增强蛋白/肽抗原免疫原性的方法 |
| AU2022207985A1 (en) | 2021-01-13 | 2023-07-06 | Visterra, Inc. | Humanized complement 5a receptor 1 antibodies and methods of use thereof |
Family Cites Families (37)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB8823869D0 (en) | 1988-10-12 | 1988-11-16 | Medical Res Council | Production of antibodies |
| US5625126A (en) | 1990-08-29 | 1997-04-29 | Genpharm International, Inc. | Transgenic non-human animals for producing heterologous antibodies |
| US5814318A (en) | 1990-08-29 | 1998-09-29 | Genpharm International Inc. | Transgenic non-human animals for producing heterologous antibodies |
| US5770429A (en) | 1990-08-29 | 1998-06-23 | Genpharm International, Inc. | Transgenic non-human animals capable of producing heterologous antibodies |
| US5661016A (en) | 1990-08-29 | 1997-08-26 | Genpharm International Inc. | Transgenic non-human animals capable of producing heterologous antibodies of various isotypes |
| US5789650A (en) | 1990-08-29 | 1998-08-04 | Genpharm International, Inc. | Transgenic non-human animals for producing heterologous antibodies |
| ATE300615T1 (de) | 1990-08-29 | 2005-08-15 | Genpharm Int | Transgene mäuse fähig zur produktion heterologer antikörper |
| US5874299A (en) | 1990-08-29 | 1999-02-23 | Genpharm International, Inc. | Transgenic non-human animals capable of producing heterologous antibodies |
| US5545806A (en) | 1990-08-29 | 1996-08-13 | Genpharm International, Inc. | Ransgenic non-human animals for producing heterologous antibodies |
| US6300129B1 (en) | 1990-08-29 | 2001-10-09 | Genpharm International | Transgenic non-human animals for producing heterologous antibodies |
| US5877397A (en) | 1990-08-29 | 1999-03-02 | Genpharm International Inc. | Transgenic non-human animals capable of producing heterologous antibodies of various isotypes |
| US5633425A (en) | 1990-08-29 | 1997-05-27 | Genpharm International, Inc. | Transgenic non-human animals capable of producing heterologous antibodies |
| AU2235992A (en) | 1991-06-14 | 1993-01-12 | Genpharm International, Inc. | Transgenic immunodeficient non-human animals |
| DE69224906T2 (de) | 1991-07-08 | 1998-10-29 | Univ Massachusetts | Thermotropes flüssig-kristallines segment-blockcopolymer |
| CA2161351C (en) | 1993-04-26 | 2010-12-21 | Nils Lonberg | Transgenic non-human animals capable of producing heterologous antibodies |
| DE60037896D1 (de) | 1999-07-29 | 2008-03-13 | Medarex Inc | Menschliche antikörper gegen her2/neu |
| CN101928344B (zh) | 2002-10-17 | 2014-08-13 | 根马布股份公司 | 抗cd20的人单克隆抗体 |
| US8398980B2 (en) * | 2004-03-24 | 2013-03-19 | Chugai Seiyaku Kabushiki Kaisha | Subtypes of humanized antibody against interleuken-6 receptor |
| EP2567976B1 (en) | 2005-03-23 | 2017-07-19 | Genmab A/S | Antibodies against CD38 for treatment of multiple myeloma |
| JP2009544760A (ja) * | 2006-01-23 | 2009-12-17 | レコファーマ アーベー | 酵母におけるオリゴマンノースまたはヒト様グリカンを担持するタンパク質の産生およびその使用 |
| CA2644903A1 (en) | 2006-03-10 | 2007-09-20 | Macrogenics, Inc. | Identification and engineering of antibodies with variant heavy chains and methods of using same |
| EP1878747A1 (en) | 2006-07-11 | 2008-01-16 | greenovation Biotech GmbH | Glyco-engineered antibodies |
| EP2087111A2 (en) | 2007-03-19 | 2009-08-12 | Medimmune Limited | Polypeptide variants |
| US8933197B2 (en) * | 2007-08-15 | 2015-01-13 | Amunix Operating Inc. | Compositions comprising modified biologically active polypeptides |
| EP2031064A1 (de) * | 2007-08-29 | 2009-03-04 | Boehringer Ingelheim Pharma GmbH & Co. KG | Verfahren zur Steigerung von Proteintitern |
| CA2809369A1 (en) * | 2010-08-27 | 2012-03-01 | Stem Centrx, Inc. | Notum protein modulators and methods of use |
| CA2810016A1 (en) * | 2010-09-03 | 2012-03-08 | Stem Centrx, Inc. | Novel modulators and methods of use |
| WO2012118547A1 (en) * | 2010-12-08 | 2012-09-07 | Stem Centrx, Inc. | Novel modulators and methods of use |
| SA112330278B1 (ar) * | 2011-02-18 | 2015-10-09 | ستيم سينتركس، انك. | مواد ضابطة جديدة وطرق للاستخدام |
| UA117901C2 (uk) * | 2011-07-06 | 2018-10-25 | Ґенмаб Б.В. | Спосіб посилення ефекторної функції вихідного поліпептиду, його варіанти та їх застосування |
| EP2729496B8 (en) | 2011-07-06 | 2017-10-18 | Genmab A/S | Modulation of complement-dependent cytotoxicity through modifications of the c-terminus of antibody heavy chains |
| US20130058947A1 (en) * | 2011-09-02 | 2013-03-07 | Stem Centrx, Inc | Novel Modulators and Methods of Use |
| US10344050B2 (en) * | 2011-10-27 | 2019-07-09 | Genmab A/S | Production of heterodimeric proteins |
| FR3003171B1 (fr) * | 2013-03-15 | 2015-04-10 | Lab Francais Du Fractionnement | Nouveaux medicaments comprenant une composition d'anticorps enrichie en isoforme de charge majoritaire |
| SG10201912565QA (en) * | 2016-09-29 | 2020-02-27 | Amgen Inc | Low-viscosity antigen binding proteins and methods of making them |
| SG11201903693QA (en) * | 2016-11-01 | 2019-05-30 | Genmab Bv | Polypeptide variants and uses thereof |
| KR20250140128A (ko) * | 2017-02-10 | 2025-09-24 | 젠맵 비. 브이 | 폴리펩티드 변이체 및 그의 용도 |
-
2012
- 2012-07-06 EP EP12731507.5A patent/EP2729496B8/en active Active
- 2012-07-06 US US14/130,580 patent/US10323081B2/en active Active
- 2012-07-06 WO PCT/EP2012/063338 patent/WO2013004841A1/en not_active Ceased
- 2012-07-06 JP JP2014517838A patent/JP6454547B2/ja active Active
-
2018
- 2018-08-16 JP JP2018153058A patent/JP6718488B2/ja active Active
-
2019
- 2019-04-30 US US16/399,424 patent/US11795214B2/en active Active
-
2022
- 2022-08-12 JP JP2022128990A patent/JP7655886B2/ja active Active
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