JP2014521350A - 電場印加装置を用いて組織を構築するシステム及び方法 - Google Patents
電場印加装置を用いて組織を構築するシステム及び方法 Download PDFInfo
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Abstract
Description
Claims (25)
- 細胞から組織を構築する電場処理システムであって:
上で前記組織が構築される基板を受ける第1端部を有する処理チャンバ;
前記基板付近に設けられて、前記基板と該基板付近の処理チャンバの領域に電場を印加するように構成される電場印加装置;
前記電場印加装置の少なくとも1つに電気バイアスを供給するように構成される分配バイアスユニット;
前記組織を構築する際に用いられる細胞と流体を供給する流体供給システム;
前記基板に対する前記電場印加装置の位置を設定するマニピュレータ;
前記分配バイアスユニットに電力を供する少なくとも1つの電源;並びに、
前記電場印加装置、前記マニピュレータ、及び前記少なくとも1つの電源を制御する制御システム;
を有する処理システム。 - 前記電場印加装置が、前記基板の反対側である前記処理チャンバの第2端部に設けられる、請求項1に記載の処理システム。
- 前記電場印加装置の少なくとも1つが、所定の挙動を前記細胞及び/又は流体へ与えるように構成され、かつ、前記細胞及び/又は流体とは異なる挙動を与える他の電場印加装置と交換可能である、請求項1又は2に記載の処理システム。
- 前記組織を構築する細胞を供するために前記流体供給システムと流体をやり取りする細胞容器をさらに有する、請求項1乃至3のうちいずれか一項に記載の処理システム。
- 前記流体供給システムへヒドロゲルを供するヒドロゲル容器をさらに有する、請求項1乃至4のうちいずれか一項に記載の処理システム。
- 前記流体供給システムへ安定化液体を供する安定化液体容器をさらに有する、請求項1乃至5のうちいずれか一項に記載の処理システム。
- 前記流体供給システムへ洗浄液体を供する洗浄液体容器をさらに有する、請求項1乃至6のうちいずれか一項に記載の処理システム。
- 前記基板及び/又は組織を照射するように構成される少なくとも1つの電磁放射線源をさらに有する、請求項1乃至7のうちいずれか一項に記載の処理システム。
- 前記少なくとも1つの電磁放射線源が赤外放射線源である、請求項8に記載の処理システム。
- 前記少なくとも1つの電磁放射線源が紫外放射線源である、請求項8又は9に記載の処理システム。
- 前記少なくとも1つの電磁放射線源が可視光放射線源である、請求項8乃至10のうちいずれか一項に記載の処理システム。
- 前記少なくとも1つの電磁放射線源がマイクロ波放射線源である、請求項8乃至11のうちいずれか一項に記載の処理システム。
- 前記基板及び/又は細胞の温度を時空間的に変化させる温度制御システムをさらに有する、請求項1乃至12のうちいずれか一項に記載の処理システム。
- 前記制御システムが前記温度制御システムを制御する、請求項13に記載の処理システム。
- 前記流体供給システムが少なくとも1つのマイクロ流体デバイスを含む、請求項1乃至14のうちいずれか一項に記載の処理システム。
- 前記マニピュレータが、前記電場印加装置と前記基板との間の距離を変化させるように構成される、請求項1乃至15のうちいずれか一項に記載の処理システム。
- 前記マニピュレータが、前記基板に対する前記電場印加装置の方位方向を変化させるように構成される、請求項1乃至16のうちいずれか一項に記載の処理システム。
- 前記前記電場印加装置の少なくとも1つが、前記処理領域から電気的に絶縁される複数のマイクロ電極を有する、請求項1乃至17のうちいずれか一項に記載の処理システム。
- 前記電場が時間変化及び/又は空間変化する、請求項1乃至18のうちいずれか一項に記載の処理システム。
- 前記流体供給システムを介して前記処理領域へ処理媒体を供給する工程;
前記流体供給システムを介して前記処理領域へ細胞を供給する工程;及び、
前記電場印加装置の少なくとも1つに第1電気バイアスを印加することで、前記処理領域内の細胞を制御可能なように選択、輸送、配向、配列、又は操作することで前記基板上に前記組織を構築するように構成される第1電場を発生させる段階;
を有する方法。 - 電場印加装置を供する段階をさらに有する請求項20に記載の方法であって、
前記電場処理システムは:
上で前記組織が構築される基板を受ける第1端部を有する処理チャンバ;
前記基板付近に設けられて、前記基板と該基板付近の処理チャンバの領域に電場を印加するように構成される電場印加装置;
前記電場印加装置の少なくとも1つに電気バイアスを供給するように構成される分配バイアスユニット;
前記組織を構築する際に用いられる細胞と流体を供給する流体供給システム;
前記基板に対する前記電場印加装置の位置を設定するマニピュレータ;
前記分配バイアスユニットに電力を供する少なくとも1つの電源;並びに、
前記電場印加装置、前記マニピュレータ、及び前記少なくとも1つの電源を制御する制御システム;
を有する、
方法。 - 前記電場印加装置の少なくとも1つに電気バイアスを印加することで、前記処理領域内の細胞を制御可能なように選択、輸送、配向、配列、又は操作することで前記基板上に前記組織を構築するように構成される前記第1電場とは異なる第2電場を発生させる工程をさらに有する、請求項20又は21に記載の方法。
- 前記マニピュレータを用いることによって前記基板から所定の距離で前記電場印加装置を位置設定する工程をさらに有する、請求項20乃至22のうちいずれか一項に記載の方法。
- 前記マニピュレータを用いることによって前記基板に対して所定の方位方向に前記電場印加装置を位置設定する工程をさらに有する、請求項20乃至23のうちいずれか一項に記載の方法。
- 赤外放射線、可視光放射線、紫外放射線、及びマイクロ波放射線のうちの少なくとも1つを前記組織に照射する工程をさらに有する、請求項24に記載の方法。
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PCT/US2012/049056 WO2013019814A2 (en) | 2011-08-02 | 2012-07-31 | System and method for tissue construction using an electric field applicator |
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JP6796932B2 (ja) * | 2016-02-16 | 2020-12-09 | 株式会社Afiテクノロジー | 分離装置 |
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JP6114270B2 (ja) | 2017-04-12 |
EP2740143A2 (en) | 2014-06-11 |
US20130192990A1 (en) | 2013-08-01 |
WO2013019814A2 (en) | 2013-02-07 |
US8916055B2 (en) | 2014-12-23 |
TWI486995B (zh) | 2015-06-01 |
US20130217210A1 (en) | 2013-08-22 |
JP2014532111A (ja) | 2014-12-04 |
TWI507349B (zh) | 2015-11-11 |
WO2013019810A9 (en) | 2013-06-20 |
WO2013019810A2 (en) | 2013-02-07 |
JP6047159B2 (ja) | 2016-12-21 |
US20150152556A1 (en) | 2015-06-04 |
EP2739719A2 (en) | 2014-06-11 |
US9228261B2 (en) | 2016-01-05 |
WO2013019810A3 (en) | 2013-05-02 |
WO2013019814A3 (en) | 2013-08-01 |
TW201313604A (zh) | 2013-04-01 |
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