JP2014509397A - 磁気フローサイトメトリー装置、磁気フローサイトメトリー装置の製造方法および磁気式細胞検出方法 - Google Patents
磁気フローサイトメトリー装置、磁気フローサイトメトリー装置の製造方法および磁気式細胞検出方法 Download PDFInfo
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Abstract
【選択図】図1
Description
1.細胞サンプルがマイクロ流体流路を通してポンピングされる速度
2.印加される富化磁界の磁界強度
3.懸濁液中における超常磁性にマーキングされた細胞の濃度、並びに
4.使用されるマーカーの磁気特性
5.細胞懸濁液の組成及びレオロジー特性、すなわち例えばその流動特性、及び
6.マーキングされた細胞の種類、及び細胞表面上のアイソトープ数、及び従って検出すべき漂遊磁界の強度を決定する細胞当りの常磁性マーカーの数
K1、K2 富化区間の湾曲部
11 マイクロ流体流路への入口
12 基板(半導体チップ)
13 担体
15 磁気ガイド条片(磁気ガイド路)
16 パッケージング材料
20 センサ
50 マイクロ流体流路
90 磁気的にマーキングされた細胞
600 富化区間の小部分
601 富化区間に対するオフセット
磁気フローサイトメトリー装置に関する本発明の有利な実施態様は次の通りである。
・富化区間は磁気ガイド条片を有する(請求項2)。
・磁気ガイド条片は強磁性である(請求項3)。
・基板、特に半導体基板を有し、磁気抵抗センサ、特にマイクロ流体流路であるフロー室及び富化区間は基板上に配置されている(請求項4)。
・マイクロ流体流路であるフロー室は、マイクロ流体流路を流れる磁気的にマーキングされた細胞サンプルが磁気ガイド条片で方向付けられるように富化区間に沿って配置されている(請求項5)。
・磁石を有し、磁石は、マイクロ流体流路であるフロー室を流れる磁気的にマーキングされた細胞サンプルが磁石の磁界によって流路底面において富化されるように配置されている(請求項6)。
・マイクロ流体流路であるフロー室及び磁気抵抗センサは、マイクロ流体流路を流れる磁気的にマーキングされた細胞サンプルがセンサ上に導かれるように配置されている(請求項7)。
・富化区間は少なくとも12500μmの区間長さの値を有する(請求項8)。
・基板の最大広がりは最大18000μmである(請求項9)。
・磁気抵抗センサは、巨大磁気抵抗(GMR)センサ、トンネル磁気抵抗(TMR)センサ又は異方性磁気抵抗(AMR)センサを有する(請求項10)。
装置の製造方法は、
磁気抵抗センサを基板上に作成するステップ、
基板上へ磁気ガイド条片を設置するステップ、
基板上にマイクロ流体流路であるフロー室を取り付けるステップ、
を含む磁気フローサイトメトリー装置の製造方法である(請求項11)。
磁気フローサイトメトリー装置の製造方法に関する本発明の実施態様は次の通りである。
・磁気抵抗センサは半導体基板上に集積される(請求項12)。
・磁気ガイド条片は基板上へ直接、特に熱蒸発又はスパッタリングによって堆積される(請求項13)。
さらに、本願発明によれば、磁気的にマーキングされた細胞サンプルが本発明による磁気フローサイトメトリー装置へ注入される磁気式細胞検出方法が提案される(請求項14)。
Claims (14)
- 磁気抵抗センサ(20)、細胞サンプルが通流するように形成されたフロー室(50)、及び富化区間(10)を有し、富化区間(10)は蛇行状に形成されている磁気フローサイトメトリー装置。
- 富化区間(10)は磁気ガイド条片(15)を有する請求項1記載の装置。
- 磁気ガイド条片(15)は強磁性である請求項2記載の装置。
- 基板(12)、特に半導体基板を有し、磁気抵抗センサ(20)、特にマイクロ流体流路であるフロー室(50)及び富化区間(10)は基板(12)上に配置されている請求項1から3のいずれか1項に記載の装置。
- マイクロ流体流路(50)は、マイクロ流体流路(50)を流れる磁気的にマーキングされた細胞サンプル(90)が磁気ガイド条片(15)で方向付けられるように富化区間(10)に沿って配置されている請求項2から4のいずれか1項に記載の装置。
- 磁石を有し、磁石は、マイクロ流体流路(50)を流れる磁気的にマーキングされた細胞サンプル(90)が磁石の磁界によって流路底面において富化されるように配置されている請求項1から5のいずれか1項に記載の装置。
- マイクロ流体流路(50)及び磁気抵抗センサ(20)は、マイクロ流体流路(50)を流れる磁気的にマーキングされた細胞サンプル(90)がセンサ(20)上に導かれるように配置されている請求項1から7のいずれか1項に記載の装置。
- 富化区間(10)は少なくとも12500μmの値を有する請求項1から7のいずれか1項に記載の装置。
- 基板(12)の最大広がりは最大18000μmである請求項1から8のいずれか1つに記載の装置。
- 磁気抵抗センサ(20)は、GMRセンサ、TMRセンサ又はAMRセンサを有する請求項1から9のいずれか1項に記載の装置。
- 以下のステップ、
基板(12)上に磁気抵抗センサ(20)を作成、
基板(12)上へ磁気ガイド条片(15)を設置、
センサ(12)上にマイクロ流体流路(50)を取り付け、
を含む請求項1から10のいずれか1項に記載の装置の製造方法。 - 磁気抵抗センサ(20)は半導体基板(12)上に集積される請求項11記載の方法。
- 磁気ガイド条片(15)は基板(12)上へ直接、特に熱蒸発又はスパッタリングによって堆積される請求項11又は12記載の方法。
- 磁気的にマーキングされた細胞サンプル(90)が請求項1から10のいずれか1項に記載の装置へ注入される磁気式細胞検出方法。
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DE102011004805A DE102011004805A1 (de) | 2011-02-28 | 2011-02-28 | Miniaturisierte magnetische Durchflusszytometrie |
DE102011004805.7 | 2011-02-28 | ||
PCT/EP2012/052901 WO2012116906A1 (de) | 2011-02-28 | 2012-02-21 | Miniaturisierte magnetische durchflusszytometrie |
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Patent Citations (3)
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JPH0843378A (ja) * | 1994-03-04 | 1996-02-16 | Cleveland Clinic Found:The | 磁気的細胞測定のための方法および装置 |
US20080160630A1 (en) * | 2006-12-29 | 2008-07-03 | Intel Corporation | Enzymatic signal generation and detection of binding complexes in stationary fluidic chip |
WO2010100192A1 (de) * | 2009-03-06 | 2010-09-10 | Siemens Aktiengesellschaft | Vorrichtung und verfahren zur anreicherung und erfassung von magnetisch markierten zellen in laminar strömenden medien |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2017223570A (ja) * | 2016-06-16 | 2017-12-21 | 株式会社東芝 | センサ |
US10837953B2 (en) | 2016-06-16 | 2020-11-17 | Kabushiki Kaisha Toshiba | Sensor |
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US20130330828A1 (en) | 2013-12-12 |
KR20130123475A (ko) | 2013-11-12 |
EP2668500A1 (de) | 2013-12-04 |
CA2828288A1 (en) | 2012-09-07 |
CA2828288C (en) | 2016-08-09 |
JP5827348B2 (ja) | 2015-12-02 |
KR101548847B1 (ko) | 2015-08-31 |
RU2013143620A (ru) | 2015-04-10 |
RU2582391C2 (ru) | 2016-04-27 |
EP2668500B1 (de) | 2016-04-13 |
WO2012116906A1 (de) | 2012-09-07 |
BR112013021961A2 (pt) | 2016-11-16 |
CN103502814A (zh) | 2014-01-08 |
DE102011004805A1 (de) | 2012-08-30 |
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