JP2014200543A - Medical mixing container and method of using the same - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a medical mixing container with which a cell and an action component can be mixed without damaging the cell.SOLUTION: When a stress applying chamber 21 is pressed by applying force F from the outside of a second bag 2 and air enclosed in the stress applying chamber 21 is compressed, stress in a separating direction is applied to a sealed part via the compressed air, mutually-bonded opening peripheral edge parts 23a of a recess are separated to cancel the sealing in the sealed part, and an action component housing chamber 22 is communicated with a cell housing chamber 11 of a first bag 1. An action liquid L1 housed in the action component housing chamber 22 flows into the cell housing chamber 11, and a mixed liquid C2 obtained by mixing the action liquid L1 and a cell suspension C1 is generated in the cell housing chamber 11.

Description

  The present invention relates to a medical mixing container and a method of using the same, and more particularly, to a medical mixing container for mixing cells and active ingredients acting on the cells.

Macrophages are immune cells that differentiate from monocytes that occupy some of the white blood cells in the blood, and phagocytose and digest bacteria, viruses, and foreign bodies that have invaded the body, for example, by activating macrophages in containers, It is known that activated macrophages are isolated and used for wound treatment and the like.
The activation of macrophages can be performed, for example, by introducing and mixing an activation component such as a differentiation-inducing drug in a container containing whole blood or buffy coat.

  Conventionally, as a container capable of performing such a mixing operation, Patent Document 1 has two storage chambers, a first chamber and a second chamber, and these two storage chambers are isolated by a weak seal portion so as to communicate with each other. A return container is disclosed. For example, a weak seal portion is formed by sealing a drug in the first chamber and sealing a solution in the second chamber and pressing the outer wall of the first chamber or the second chamber to apply pressure to the weak seal portion. The first sheet and the second chamber are communicated with each other, and the solution stored in the second chamber is transferred to the first chamber and the drug is dissolved in the solution.

JP 2009-62382 A

  However, when an external force is applied to the outer wall of the first chamber or the second chamber and pressed, the external force becomes pressure and is directly applied to the medicine in the first chamber or the solution in the second chamber. Therefore, when the outer wall of the first chamber is pressed, the medicine stored in the first chamber is vigorously ejected into the second chamber when the first chamber communicates with the second chamber, while the second chamber When the outer wall is pressed, the solution stored in the second chamber is ejected vigorously into the first chamber when the first chamber communicates with the second chamber.

  For this reason, when trying to mix a cell and an active ingredient acting on a cell such as an activating component using the return chamber disclosed in Patent Document 1, there is a risk of damaging the cell due to an impact during mixing. There is a problem that there is.

The present invention has been made to solve such conventional problems, and an object of the present invention is to provide a medical mixing container capable of mixing cells and active ingredients without damaging the cells. .
Another object of the present invention is to provide a method for using such a medical mixing container.

  The medical mixing container according to the present invention is a medical mixing container for mixing cells and an active ingredient acting on the cells, and is capable of communicating with a cell storage chamber in which cells are stored, and a cell storage chamber, respectively. At least one active ingredient storage chamber in which the active ingredient is accommodated and at least one sealing portion that releasably seals between the corresponding active ingredient storage chamber and the cell storage chamber, respectively. Each of the stress applying chambers is provided with at least one stress applying chamber which encloses the corresponding active ingredient containing chamber and sandwiches the corresponding sealing portion from both sides and which contains a fluid for applying stress to the sealing portion. When the fluid in the pressure applying chamber is compressed by pressing the pressure, stress is applied to the corresponding sealing portion via the fluid, and the sealing in the sealing portion is released, so that the corresponding active ingredient containing chamber is contained in the cell collection. Communicating with the chamber, the action component of the working component storage chamber is to flow into the cell-containing chamber.

The cell storage chamber is formed of a flexible first bag having openings corresponding to the respective active ingredient storage chambers, and has a closed space inside due to the respective active ingredient storage. Are formed by recesses formed by recessing a part of the interior of the second bag, each pressure applying chamber is formed by a closed space of the second bag, and the opening peripheral edge of the recess is the first bag. Are bonded to the peripheral edge of the corresponding opening, and the opening peripheral edge of the recess is releasably bonded to each other so that the inside of the recess is sealed, and a sealing portion is formed by the opening peripheral edge of the recess that is releasably bonded Can be configured.
Preferably, the second bag has a bifurcated portion which is formed on the side opposite to the sealing portion with respect to the active ingredient storage chamber and is gripped when the stress applying chamber is compressed.
It is preferable to further include a port connected to the cell storage chamber and for taking out the cells mixed with the active ingredient in the cell storage chamber from the cell storage chamber.

  In the method of using the medical mixing container according to the present invention, the cells are stored in the cell storage chamber of the medical mixing container, and the active ingredients are stored in at least one active ingredient storage chamber. By compressing the fluid in the pressure applying chamber by pressing each pressure applying chamber, stress is applied to the corresponding sealing portion via the fluid, and the sealing in the sealing portion is released, and the corresponding active ingredient is contained. In this method, the chamber communicates with the cell storage chamber, and the active component in the active component storage chamber flows into the cell storage chamber and is mixed with the cells.

  It should be noted that different active ingredients are accommodated in a plurality of active ingredient accommodating chambers, and the pressure applying chamber corresponding to one active ingredient accommodating chamber is pressed to cause the active ingredient accommodated in the active ingredient accommodating chamber to flow into the cell accommodating chamber. Then, the pressure application chamber corresponding to the other active ingredient storage chamber can be pressed to cause the active ingredient stored in the other active ingredient storage chamber to flow into the cell storage chamber.

  According to the present invention, by compressing the fluid in the pressure applying chamber by pressing the stress applying chamber, stress is applied to the sealing portion via the fluid, and the sealing in the sealing portion is released, and the active ingredient is accommodated. Since the chamber communicates with the cell storage chamber, the active ingredient is prevented from flowing into the cell storage chamber rapidly, and the cells and the active ingredient can be mixed without damaging the cells stored in the cell storage chamber. It becomes.

It is a front view which shows the structure of the medical mixing container which concerns on Embodiment 1 of this invention. It is sectional drawing which shows the medical mixing container which concerns on Embodiment 1 of the state by which sealing by the sealing part is cancelled | released. It is a partial expanded sectional view which shows the principal part of the medical mixing container shown in FIG. It is a partial expanded sectional view which shows the principal part of the medical mixing container which concerns on Embodiment 1 of the state sealed with the sealing part. It is sectional drawing which shows the medical mixing container which concerns on Embodiment 1 of the state which accommodated the active ingredient in the active ingredient storage chamber, and accommodated the cell in the cell storage chamber. It is sectional drawing which shows the medical mixing container which concerns on Embodiment 1 of the state which the sealing | blocking in the sealing part was cancelled | released and the active ingredient accommodation chamber connected to the cell accommodation chamber. 6 is a front view showing a structure of a medical mixing container according to Embodiment 2. FIG. 6 is a cross-sectional view showing a structure of a medical mixing container according to Embodiment 2. FIG. It is sectional drawing which shows the medical mixing container which concerns on Embodiment 2 of the state with which the active ingredient accommodated in one active ingredient accommodating chamber was mixed with the cell in a cell accommodating chamber. It is sectional drawing which shows the medical mixing container which concerns on Embodiment 2 of the state with which the active ingredient accommodated in the other active ingredient storage chamber was mixed with the cell in a cell storage chamber. 6 is a front view showing a structure of a medical mixing container according to Embodiment 3. FIG. FIG. 10 is a front view showing the structure of a medical mixing container according to a modification of the third embodiment.

Embodiments of the present invention will be described below with reference to the accompanying drawings.
Embodiment 1
FIG. 1 shows the configuration of the medical mixing container according to the first embodiment. The medical mixing container has a flexible first bag 1 and a flexible second bag 2 connected to the first bag 1. A cell storage chamber 11 is formed inside the first bag 1, and a stress applying chamber 21 and an active ingredient storage chamber 22 are formed inside the second bag 2. The active ingredient storage chamber 22 is enclosed by the stress applying chamber 21 and is disposed so as to be able to communicate with the cell storage chamber 11. The sealing portion 3 is formed between the active ingredient storage chamber 22 and the cell storage chamber 11. . The sealing part 3 seals between the active ingredient storage chamber 22 and the cell storage chamber 11 so as to be releasable.
Further, the first bag 1 is provided with a port 12 for supplying cells to the cell storage chamber 11 and taking out the cells from the cell storage chamber 11.

FIG. 2 shows a cross-sectional view of the state where the sealing by the sealing portion 3 is released. The second bag 2 has a closed space inside, and a stress applying chamber 21 is formed by the closed space, and the active ingredient storage chamber 22 is a part of the second bag 2 and the second bag 2. It is formed in the inside of the recessed part 23 formed by being depressed inside. Inside the stress applying chamber 21, air is sealed as a fluid for applying stress to the sealing portion 3.
As shown in an enlarged view in FIG. 3, the first bag 1 is formed with an opening 13 corresponding to the active ingredient storage chamber 22 at a connection portion with the second bag 2, and the second bag 1. The open peripheral edge 23a of the two recesses 23 is joined to the peripheral edge 13a of the opening 13 of the first bag 1, so that the active ingredient storage chamber 22 of the second bag 2 becomes the first bag. One cell storage chamber 11 communicates.

  And as FIG. 4 shows, the opening peripheral part 23a which the recessed part 23 was open | released in the state which accommodated the action | operation liquid L1 in which the active ingredient for activating a cell was accommodated in the inside of the active ingredient accommodation chamber 22 Are detachably joined to each other so that the active ingredient storage chamber 22 in the recess 23 is hermetically sealed and the sealing portion 3 that seals between the active ingredient storage chamber 22 and the cell storage chamber 11 is formed. . At this time, the stress applying chamber 21 in the second bag 2 in which air is sealed encloses the periphery of the active ingredient storage chamber 22 and sandwiches the sealing portion 3 from both sides.

  The first bag 1 and the second bag 2 are soft resin materials having drug resistance and flexibility, such as polyolefin (polyethylene, polypropylene, polybutene, ethylene-propylene copolymer, ethylene-vinyl acetate copolymer). , Ionomers, or a mixture of two or more thereof), polyvinyl chloride, polyamide, polyamide elastomer, polyester, polyester elastomer, polyurethane, polyurethane elastomer, polyimide, fluororesin, or a mixture thereof, or the above 2 It can be formed from more than one polymeric material.

The bonding between the opening peripheral edge 23a of the recess 23 of the second bag 2 and the peripheral edge 13a of the opening 13 of the first bag 1 is performed by connecting the resin materials forming the second bag 2 and the first bag 1 to each other. This can be done by heat-sealing or by bonding via an adhesive.
On the other hand, the joining of the opening peripheral portion 23a of the concave portion 23 in the sealing portion 3 is separated from the opening peripheral portion 23a of the concave portion 23 of the second bag 2 and the first bag so as to be peeled off when a predetermined stress is applied. 1 has a smaller bonding force than the bonding with the peripheral edge portion 13a of the opening 13. Such a weak bond can be formed by heat-sealing the opening peripheral edge 23a of the recess 23 at a relatively low temperature. Alternatively, a resin sheet material that softens at a lower temperature than the resin material that forms the first bag 1 and the second bag 2 is sandwiched between the opening peripheral portions 23a of the recesses 23 facing each other, and heated. You may melt | fuse both surfaces of the resin sheet material to the opening peripheral part 23a of both sides, respectively.

Next, a method of mixing an active ingredient into cells using such a medical mixing container will be described.
It is assumed that the working liquid L1 is accommodated in the active ingredient accommodating chamber 22 of the second bag 2 in advance, and the gap between the active ingredient accommodating chamber 22 and the cell accommodating chamber 11 is sealed by the sealing portion 3. . As shown in FIG. 5, the cell suspension C <b> 1 containing cells is accommodated in the cell accommodating chamber 11 of the first bag 1 through the port 12 attached to the first bag 1.

  Next, as shown in FIG. 6, a force F is applied from the outside of the second bag 2 to press the stress applying chamber 21, and the air sealed in the pressure applying chamber 21 is compressed. At this time, since the stress applying chamber 21 wraps around the active ingredient housing chamber 22 and sandwiches the sealing portion 3 from both sides, the opening peripheral portion 23a of the recess 23 is formed by the compression of the air in the pressure applying chamber 21. A stress in a peeling direction is applied to the sealing portion 3 through the compressed air so that the open state shown in FIGS. When this stress exceeds the bonding force of the opening peripheral edge 23a of the recess 23, the opening peripheral edge 23a that has been bonded to each other is peeled off, the sealing in the sealing portion 3 is released, and the active ingredient accommodating chamber 22 is in the first state. It will be in the state connected to the cell storage chamber 11 of the bag 1.

Thereby, the working fluid L1 accommodated in the active ingredient housing chamber 22 flows into the cell housing chamber 11, and a mixed solution C2 in which the cell suspension C1 and the working fluid L1 are mixed is generated in the cell housing chamber 11. Then, the cells contained in the cell suspension C1 are activated by the active component in the working fluid L1.
In addition, by pressing the stress applying chamber 21 and compressing the air enclosed in the pressure applying chamber 21, stress is applied to the sealing portion 3 through the compressed air, and the sealing in the sealing portion 3 is performed. Since the stop is released and the active ingredient storage chamber 22 communicates with the cell storage chamber 11, the pressing force to the stress applying chamber 21 is not directly transmitted to the working liquid L1 in the active ingredient storage chamber 22. The working fluid L1 is prevented from being ejected into the cell storage chamber 11 under pressure. That is, the working fluid L1 gently flows into the cell housing chamber 11 from the working component housing chamber 22, and is mixed with the cell suspension C1 without damaging the cells contained in the cell suspension C1.

Thereafter, the mixture C2 is taken out from the cell storage chamber 11 through the port 12 attached to the first bag 1, and the activated cells are isolated from the mixture C2 and used for wound treatment or the like. The
The activated cells can be taken out from the port 12 after being isolated in the cell storage chamber 11.

In the first embodiment, the port 12 attached to the first bag 1 is used to supply the cell suspension C1 into the cell storage chamber 11 and the mixture C2 or cell from the cell storage chamber 11. However, the supply port and the extraction port can be separately attached to the first bag 1.
In addition, the sealing is performed by the sealing portion 3 in a state where the working liquid storage chamber 22 of the second bag 2 is stored in advance, but the present invention is not limited to this. It is also possible to attach a supply port that communicates with the active ingredient storage chamber 22 and supply the working fluid L1 into the active ingredient storage chamber 22 that is sealed from the cell storage chamber 11 by the sealing portion 3.
Furthermore, although air is enclosed as a fluid for applying stress to the sealing portion 3 inside the stress applying chamber 21, it is not limited to air, and various gases or liquids can be used.

Embodiment 2
FIG. 7 shows the configuration of the medical mixing container according to the second embodiment. This medical mixing container is obtained by further connecting a third bag 4 similar to the second bag 2 to the first bag 1 in the medical mixing container of the first embodiment shown in FIG. A stress applying chamber 41 and an active ingredient accommodating chamber 42 are formed in the third bag 4, and the active ingredient accommodating chamber 42 is enclosed by the stress applying chamber 41 and can communicate with the cell accommodating chamber 11 of the first bag 1. The sealing portion 5 is formed between the active ingredient storage chamber 42 and the cell storage chamber 11. The sealing part 5 seals the space between the active ingredient storage chamber 42 and the cell storage chamber 11 in a releasable manner, and air is enclosed in the stress applying chamber 41.

As shown in FIG. 8, the working fluid L <b> 1 is housed in the working component housing chamber 22 of the second bag 2, and the working fluid L <b> 1 of the second bag 2 is placed in the working component housing chamber 42 of the third bag 4. It is assumed that a working fluid L2 different from that is contained. The working fluid L2 is a working component for activating cells, like the working fluid L1, but includes working components different from the working components in the working fluid L1.
When the medical mixing container according to the second embodiment is used, the cell suspension C1 is stored in the cell storage chamber 11 of the first bag 1, and as shown in FIG. A force F is applied from the outside to press the stress applying chamber 21, release the sealing in the sealing portion 3, and connect the active ingredient accommodating chamber 22 to the cell accommodating chamber 11, thereby accommodating the active ingredient accommodating chamber 22. The applied working fluid L1 is caused to flow into the cell storage chamber 11. As a result, a mixed liquid C2 in which the cell suspension C1 and the working liquid L1 are mixed is generated in the cell storage chamber 11.

Next, as shown in FIG. 10, a force F is applied from the outside of the third bag 4 to press the stress applying chamber 41, and the sealing in the sealing portion 5 is released, so that the active ingredient storage chamber 42 becomes a cell. By communicating with the storage chamber 11, the working liquid L <b> 2 stored in the active ingredient storage chamber 42 flows into the cell storage chamber 11. As a result, a mixed liquid C3 in which the working liquid L2 is further mixed with the mixed liquid C2 is generated in the cell storage chamber 11.
The cells contained in the cell suspension C1 are activated by the action of the action component in the action liquid L1 and the action component in the action liquid L2.
Thereafter, the mixed solution C3 is taken out from the cell storage chamber 11, and activated cells can be isolated from the mixed solution C3 and used for wound treatment or the like. Alternatively, activated cells may be isolated from the cell storage chamber 11 and then taken out from the cell storage chamber 11 for use.

In the second embodiment, different working fluids L1 and L2 are stored in the working component storage chamber 22 of the second bag 2 and the working component storage chamber 42 of the third bag 4. A liquid can also be accommodated.
Similarly, the three or more bags are connected to the first bag 1 so that the three or more active ingredient storage chambers communicate with the cell storage chamber 11 of the first bag 1 through the sealing portion. It can also be connected.

Embodiment 3
FIG. 11 shows the configuration of the medical mixing container according to the third embodiment. This medical mixing container is obtained by connecting the second bag 2A to the first bag 1 instead of the second bag 2 in the medical mixing container of the first embodiment shown in FIG. Similarly to the second bag 2, the second bag 2 </ b> A has a stress applying chamber 21 and an active ingredient storage chamber 22 inside thereof, and the active component storage chamber 22 and the cell storage chamber 11 of the first bag 1. The releasable sealing part 3 is formed between them, and further has a bifurcated part 24 on the side opposite to the sealing part 3 with respect to the active ingredient storage chamber 22. The bifurcated portion 24 is for gripping the stress applying chamber 21 when it is compressed.

By grasping and grasping the bifurcated portion 24 of the second bag 2A formed in this way, the air sealed in the stress applying chamber 21 is sealed on the opposite side of the bifurcated portion 24. The active ingredient accommodating chamber 22 can be communicated with the cell accommodating chamber 11 by easily releasing the sealing by the sealing portion 3.
For this reason, it becomes possible to improve the operability of the medical mixing container.

As shown in FIG. 12, the second bag 2 </ b> A and the third bag 4 </ b> A each having a bifurcated portion can be connected to the first bag 1. Similarly to the third bag 4 shown in FIG. 7, the third bag 4 </ b> A has a stress applying chamber 41 and an active ingredient accommodating chamber 42 therein, and the active ingredient accommodating chamber 42 and the cells of the first bag 1. A releasable sealing portion 5 is formed between the storage chamber 11 and the side opposite to the sealing portion 5 with respect to the active ingredient storage chamber 42 as in the second bag 2A shown in FIG. Has a bifurcated portion 44.
Using such second bag 3A and third bag 4A, as in the second embodiment, for example, different working fluids L1 and L2 are sequentially applied to the cell suspension in the cell storage chamber 11. The operation of activating cells by mixing with C1 can be performed with good operability.

For example, when activating macrophages, whole blood, buffy coat, fractionated leukocytes, etc. are used as cell suspension C1, and differentiation-inducing drugs, distilled water, A hypertonic solution, physiological saline, or the like can be used.
Moreover, as an active ingredient, not only what activates a cell but conversely, various active ingredients which have the effect on a cell, such as an active ingredient which suppresses activation of a cell, can be used.

  DESCRIPTION OF SYMBOLS 1 1st bag, 11 cell accommodation chamber, 12 ports, 13 opening part, 13a peripheral part, 2,2A 2nd bag, 21,41 stress applying chamber, 22,42 active ingredient accommodation chamber, 23 recessed part, 23a opening Peripheral part, 24, 44 Forked part, 3, 5 Sealing part, 4, 4A Third bag, L1, L2 working solution, C1 cell suspension, C2, C3 mixed solution, F force.

Claims (6)

A medical mixing container for mixing cells and active ingredients acting on the cells,
A cell storage chamber in which the cells are stored;
At least one active ingredient storage chamber that is disposed so as to communicate with the cell storage chamber and in which the active ingredient is stored;
At least one sealing portion that releasably seals between the corresponding active ingredient accommodating chamber and the cell accommodating chamber, and the corresponding sealing portion that encloses the corresponding corresponding active ingredient accommodating chamber from both sides. And at least one stress applying chamber in which a fluid for applying stress to the sealing portion is accommodated and compressing the fluid in the pressure applying chamber by pressing each of the stress applying chambers In addition, stress is applied to the corresponding sealing portion via the fluid, and the sealing of the sealing portion is released, and the corresponding active ingredient storage chamber communicates with the cell storage chamber, so that the active component storage is performed. A medical mixing container, wherein the active ingredient in the chamber flows into the cell storage chamber. The cell storage chamber is formed by a flexible first bag having an opening corresponding to each of the active ingredient storage chambers,
Each of the active ingredient storage chambers is formed by a recess formed by recessing a part of a flexible second bag having a closed space inside the second bag,
Each of the pressure applying chambers is formed by the closed space of the second bag,
The opening periphery of the recess is joined to the periphery of the corresponding opening of the first bag, and the opening periphery of the recess is joined to be peelable from each other so that the inside of the recess is sealed,
The medical mixing container according to claim 1, wherein the sealing portion is formed by an opening peripheral edge portion of the concave portion joined in a peelable manner.   3. The second bag according to claim 2, wherein the second bag has a bifurcated portion that is formed on a side opposite to the sealing portion with respect to the active ingredient storage chamber and is gripped when the stress applying chamber is compressed. Medical mixing container.   The port according to any one of claims 1 to 3, further comprising a port connected to the cell storage chamber and for taking out the cell mixed with the active ingredient in the cell storage chamber from the cell storage chamber. Medical mixing container. The cell is accommodated in the cell accommodating chamber of the medical mixing container according to any one of claims 1 to 4, and the active ingredient is accommodated in the at least one active ingredient accommodating chamber, respectively.
By compressing each of the pressure applying chambers and compressing the fluid in the pressure applying chambers, stress is applied to the corresponding sealing portion via the fluid, and the sealing in the sealing portion is released. The corresponding active ingredient accommodating chamber communicates with the cell accommodating chamber, and the active ingredient in the active ingredient accommodating chamber flows into the cell accommodating chamber and is mixed with the cells. How to use. Containing different active ingredients in the plurality of active ingredient storage chambers;
After pressing the pressure applying chamber corresponding to one of the active ingredient accommodating chambers to cause the active ingredient accommodated in the active ingredient accommodating chamber to flow into the cell accommodating chamber, the other active ingredient accommodating chambers The method for using a medical mixing container according to claim 5, wherein the corresponding pressure application chamber is pressed to cause the active ingredient contained in another active ingredient accommodating chamber to flow into the cell accommodating chamber.

Images