JP2014171389A5

JP2014171389A5 -

Info

Publication number: JP2014171389A5
Application number: JP2013043424A
Authority: JP
Filing date: 2013-03-05
Publication date: 2016-02-12
Anticipated expiration: 2033-03-05

Description

本実施例の第１基板１１と第３基板５１は、PDMSから成る20mm角の基板である。
第１基板１１は、厚み1.3mmであり、中央には直径2.5mmの貫通孔１２が形成されている。ここで、第１基板１１において、貫通孔１２の中心軸側を基板の中心側、その反対を外側とし、培養時に細胞培養ディッシュ２１と接する側の面を底面、その反対の面を上面とする。第１基板１１の上面には、2本の流路（幅0.5mm深さ0.2mm）１７ａ及び１７ｂが形成されており、各流路１７ａ、１７ｂの一端は貫通孔１２へ連通している。第１基板１１の底面には、貫通孔１２を取り囲むように環状凹部１４が形成されている。貫通孔１２は、培養室の壁面に起因する影によって培養室７１内の細胞が観察できないことがないように、観察に用いる顕微鏡のレンズの性能に合わせて、すり鉢状とすることが望ましい。 The first substrate 11 and the third substrate 51 of the present embodiment are 20 mm square substrates made of PDMS.
The first substrate 11 has a thickness of 1.3 mm, and a through hole 12 having a diameter of 2.5 mm is formed at the center. Here, in the first substrate 11, the central axis side of the through hole 12 is the center side of the substrate, the opposite is the outer side, the surface in contact with the cell culture dish 21 during culture is the bottom surface, and the opposite surface is the upper surface. . Two channels (width 0.5 mm, depth 0.2 mm) 17 a and 17 b are formed on the upper surface of the first substrate 11, and one ends of the channels 17 a and 17 b communicate with the through hole 12. An annular recess 14 is formed on the bottom surface of the first substrate 11 so as to surround the through hole 12. It is desirable that the through-hole 12 has a mortar shape according to the performance of the lens of the microscope used for observation so that the cells in the culture chamber 71 cannot be observed due to shadows caused by the wall surface of the culture chamber.

第３基板５１は、厚み3mmであり、内部に内径6mm外径10mm厚さ2mmのネオジム磁石３１（第１保持部材に該当する）が封入されている。第３基板５１には、ネオジム磁石３１より外側の部分に2つの孔（直径1.5mm）５２ａ及び５２ｂが形成されており、本細胞培養デバイス７０を組み立てると、この孔５２ａ、５２ｂは、それぞれ、流路１７ａ、１７ｂの他端に連通する。
第１基板１１と第３基板５１は、例えば真空プラズマなどで表面を活性化させて接合されている。
The third substrate 51 has a thickness of 3 mm and encloses a neodymium magnet 31 (corresponding to the first holding member) having an inner diameter of 6 mm, an outer diameter of 10 mm, and a thickness of 2 mm. Two holes (diameter 1.5 mm) 52a and 52b are formed in the third substrate 51 on the outer side of the neodymium magnet 31, and when the cell culture device 70 is assembled, the holes 52a and 52b are respectively It communicates with the other ends of the flow paths 17a and 17b.
The first substrate 11 and the third substrate 51 are bonded by activating their surfaces with, for example, vacuum plasma.

Claims

A first substrate having a through hole;
A second substrate disposed on one side of the first substrate so as to close one end of the through hole;
A first holding member and a second holding member, which are magnetic pairs for joining the first substrate and the second substrate by a magnetic attractive force, and the first holding member is annularly formed so as to surround the through hole. cell culture device disposed on the first substrate side, and the second holding member is characterized by being disposed on the second substrate side.