JP2014138609A - Container-packed sparkling beverage - Google Patents
Container-packed sparkling beverage Download PDFInfo
- Publication number
- JP2014138609A JP2014138609A JP2014047393A JP2014047393A JP2014138609A JP 2014138609 A JP2014138609 A JP 2014138609A JP 2014047393 A JP2014047393 A JP 2014047393A JP 2014047393 A JP2014047393 A JP 2014047393A JP 2014138609 A JP2014138609 A JP 2014138609A
- Authority
- JP
- Japan
- Prior art keywords
- mass
- component
- container
- beverage
- sparkling beverage
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
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- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims abstract description 16
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
- A23L2/66—Proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
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- C12G3/022—Preparation of other alcoholic beverages by fermentation of botanical family Poaceae, e.g. wheat, millet, sorghum, barley, rye, or corn of botanical genus Oryza, e.g. rice
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Abstract
Description
本発明は、容器詰発泡飲料に関する。 The present invention relates to a packaged sparkling beverage.
炭酸飲料は、飲用中及び飲用後において、口腔内の刺激や喉通過時の刺激感(喉越し)により、爽快感や清涼感を醸成する嗜好性の高い飲料である。炭酸飲料には香味を付与するために酸味料や甘味料が配合されることがあるが、このような炭酸飲料を特に汗をかいたときなど水分を欲するときに飲用すると、酸味により喉に強い刺激が感じられてゴクゴク飲み難く、また甘味により喉に引っかかる感覚や後味に嫌な感覚が残りやすく喉の渇きが収まり難い。 A carbonated beverage is a highly palatable beverage that nurtures a refreshing feeling and a refreshing feeling due to irritation in the oral cavity and a feeling of irritation when passing through the throat (over the throat) during and after drinking. Acidic and sweeteners are sometimes added to carbonated beverages to add flavor, but if you drink such carbonated beverages when you want moisture, such as when you sweat, it is strong against the throat due to acidity. It feels irritating and it is difficult to drink, and the sweetness tends to leave a feeling of being caught in the throat and an unpleasant sensation in the aftertaste.
また、サポニンを添加することにより、ビールのような泡立ちと泡の持続性を付与した技術が提案されている(特許文献1)。一方、サポニンは苦味を有するため、風味低下を改善するべくサポニンとオリゴ糖を組み合わせる技術があるが(特許文献2)、泡の持続性が不十分であった。そこで、例えば、平均分子量20000以下のコラーゲンペプチドとサポニンを含有する発泡性飲料が提案されている(特許文献3)。 Moreover, the technique which provided the persistence of foaming and foam like beer by adding saponin is proposed (patent document 1). On the other hand, since saponin has a bitter taste, there is a technique for combining saponin and an oligosaccharide to improve a decrease in flavor (Patent Document 2), but the persistence of foam was insufficient. Therefore, for example, a sparkling beverage containing a collagen peptide having an average molecular weight of 20000 or less and saponin has been proposed (Patent Document 3).
しかしながら、前記発泡性飲料は泡立ちや泡持ちが改善されているものの、汗をかいたときの喉越しが不十分で、しかも喉の渇きが収まり難く、水分を欲するときに飲む止渇飲料として必ずしも適していなかった。
したがって、本発明の課題は、喉越しが良好でかつ酸味感が緩和されていることにより、汗をかいて水分を欲するときに飲む止渇飲料として有用な容器詰発泡飲料を提供することにある。
However, although the effervescent beverage has improved foaming and foam retention, it is not sufficient as a thirsty beverage to drink when it wants moisture, because it does not have enough thirst when sweating, and thirst is hard to settle. It was not suitable.
Accordingly, an object of the present invention is to provide a packaged sparkling beverage that is useful as a thirsty beverage to drink when sweating and wanting moisture, because the throat feel is good and the sourness is alleviated. .
本発明者は、上記課題に鑑み種々検討した結果、発泡飲料において、植物材料由来のたんぱく質を含有させ、かつ当該たんぱく質に占める特定のペプチドの割合、当該ペプチドと特定成分との質量比、及びpHを特定範囲内に制御することで、酸味感が緩和され、汗をかいたときでも喉越しが良好であり、汗をかいて水分を欲するときに飲む止渇飲料として適した容器詰発泡飲料が得られることを見出した。 As a result of various investigations in view of the above problems, the present inventor has included a protein derived from plant material in a sparkling beverage and the ratio of a specific peptide in the protein, the mass ratio of the peptide and the specific component, and pH Is controlled within a specific range, sourness is alleviated, the throat feels good even when sweating, and a packaged sparkling beverage suitable as a thirsty beverage to drink when sweating and wanting moisture It was found that it can be obtained.
すなわち、本発明は、次の成分(A)、(B)及び(C);
(A)穀物由来たんぱく質
(B)カリウム、及び
(C)起泡剤
を含有し、成分(A)に占める(D)分子量5000以下のペプチドの割合が70〜100質量%であり、成分(D)と成分(B)との含有質量比[(D)/(B)]が15〜300であり、pHが3〜5である容器詰発泡飲料を提供するものである。
That is, the present invention includes the following components (A), (B) and (C);
(A) Grain-derived protein (B) Potassium and (C) Foaming agent, (D) The proportion of peptides having a molecular weight of 5000 or less in component (A) is 70 to 100% by mass, and component (D ) And the component (B) content mass ratio [(D) / (B)] is 15 to 300, and a packaged sparkling beverage having a pH of 3 to 5 is provided.
本発明によれば、喉越しが良好でかつ酸味感が緩和されていることにより、汗をかいて水分を欲するときに飲む止渇飲料として有用な容器詰発泡飲料を提供することができる。 ADVANTAGE OF THE INVENTION According to this invention, since the feeling over a throat is good and the acidity is eased, the container-packed sparkling beverage useful as a thirsty beverage to drink when sweating and wanting water can be provided.
本発明の容器詰発泡飲料は、成分(A)として穀物由来のたんぱく質を含有する。
本発明で使用する(A)穀物由来たんぱく質は、当該たんぱく質中に(D)分子量5000以下のペプチドを70〜100質量%含有するものであるが、酸味感の緩和及び喉越し改善の点から、下限は71質量%、更に72質量%、更に73質量%、更に75質量%、更に76質量%、更に77質量%、更に80質量%が好ましく、他方上限は97質量%、95質量%、更に92質量%、更に90質量%、更に87質量%、更に86質量%が好ましい。かかる(A)穀物由来たんぱく質中の(D)分子量5000以下のペプチドの割合の範囲としては、70〜90質量%、更に71〜87質量%、更に72〜86質量%、殊更に76〜86質量%が好ましい。なお、ペプチド含有量の測定方法は、後掲の実施例に記載の方法にしたがうものとする。
The container-packed sparkling beverage of the present invention contains a protein derived from grains as a component (A).
The (A) cereal protein used in the present invention contains 70 to 100% by mass of a peptide having a molecular weight of 5000 or less in the protein (D), but from the viewpoint of alleviation of sourness and improvement over the throat, The lower limit is preferably 71% by mass, further 72% by mass, further 73% by mass, further 75% by mass, further 76% by mass, further 77% by mass, and further 80% by mass, while the upper limit is 97% by mass, 95% by mass, It is preferably 92% by mass, more preferably 90% by mass, further 87% by mass, and further 86% by mass. The range of the proportion of (D) peptides having a molecular weight of 5000 or less in the (A) protein derived from cereal is 70 to 90% by mass, further 71 to 87% by mass, further 72 to 86% by mass, particularly 76 to 86% by mass. % Is preferred. In addition, the measuring method of peptide content shall follow the method as described in an Example mentioned later.
このような(A)穀物由来たんぱく質は、たんぱく質を含有する穀物から抽出して得ることができる。
(A)穀物由来たんぱく質の原料となる穀物としては、たんぱく質を含有するものであれば特に限定されないが、酸味感の緩和及び喉越しの観点から、麦、米、コーン(とうもろこし)、豆、ゴマが好ましく、これらは1種又は2種以上を組み合わせて使用することができる。
麦としては、例えば、大麦、小麦、ハト麦が挙げられ、米としては、例えば、玄米が挙げられる。また、豆としては、例えば、大豆、黒豆、小豆、緑豆、エンドウ、インゲンが挙げられ、ゴマとしては、例えば、黒ゴマ、白ゴマ、金ゴマが挙げられる。中でも、麦、豆が好ましい。
Such (A) cereal protein can be obtained by extraction from cereal containing protein.
(A) Grain used as a raw material for protein derived from cereal is not particularly limited as long as it contains protein, but from the viewpoints of mitigation of acidity and throat, wheat, rice, corn, beans, sesame These may be used alone or in combination of two or more.
Examples of wheat include barley, wheat and pigeons, and examples of rice include brown rice. Examples of the beans include soybeans, black beans, red beans, green beans, peas, and green beans. Examples of the sesame include black sesame, white sesame, and gold sesame. Of these, wheat and beans are preferred.
(A)穀物由来たんぱく質の原料となる穀物からの抽出方法としては、攪拌抽出、カラム抽出等の公知の方法を採用することが可能であり、抽出条件も穀物の種類により適宜設定することできる。(A)穀物由来たんぱく質の形態としては、固体、水溶液、スラリー状等の種々のものがある。
また、本発明においては、抽出により得られた穀物抽出物を、必要により酵素や酸を用いて分解してもよいし、必要により、たんぱく質に占める上記分子量のペプチドの割合が上記範囲内となるように穀物抽出物を精製しても構わない。
なお、本明細書において「たんぱく質」とは、後述する「窒素定量換算法」により定量される物質から、遊離アミノ酸のみを除いたものをいう。換言すれば、本明細書における「たんぱく質」とは、技術常識でいうところのアミノ酸により構成された高分子含窒素化合物のみならず、ジペプチドまでをも含み、かつ遊離アミノ酸のみを含まない概念である。
(A) As a method for extracting from a cereal as a raw material for a protein derived from cereal, known methods such as stirring extraction and column extraction can be employed, and the extraction conditions can be appropriately set depending on the type of cereal. (A) As a form of the protein derived from a grain, there exist various things, such as solid, aqueous solution, and a slurry form.
In the present invention, the cereal extract obtained by the extraction may be decomposed using an enzyme or an acid, if necessary, and if necessary, the proportion of the peptide having the molecular weight in the protein falls within the above range. Thus, the grain extract may be purified.
In the present specification, “protein” refers to a substance obtained by removing only free amino acids from a substance quantified by the “nitrogen quantitative conversion method” described later. In other words, the term “protein” in the present specification is a concept that includes not only a high molecular nitrogen-containing compound composed of amino acids, but also dipeptides, as well as free amino acids. .
本発明においては、(A)穀物由来たんぱく質として、麦芽由来のたんぱく質を用いてもよい。麦芽由来のたんぱく質としては、例えば、麦芽抽出物を挙げることができる。麦芽抽出物とは、麦芽を糖化させて得られた液であり、具体的には、大麦等の麦類に水を加えて発芽させ、乾燥したもの(麦芽又はモルトともいう)に、温水を加えて常法で処理して麦芽に含まれる酵素の働きでデンプン質を分解(糖化)し、圧搾又は抽出して得られるものである。このような非発酵の麦芽抽出物の具体例として、麦汁が挙げられる。また、麦芽抽出物として、麦芽を糖化後に酵母を接種して発酵させたものを使用することも可能である。中でも、麦芽抽出物としては、非発酵麦芽の抽出物が好ましい。 In the present invention, malt-derived protein may be used as the (A) grain-derived protein. Examples of the malt-derived protein include a malt extract. A malt extract is a liquid obtained by saccharifying malt. Specifically, water is added to barley such as barley to germinate and dried (also referred to as malt or malt) with warm water. In addition, it is obtained by processing in a conventional manner, decomposing (saccharifying) the starch by the action of enzymes contained in the malt, and pressing or extracting. A specific example of such a non-fermented malt extract is wort. It is also possible to use a malt extract that has been fermented by inoculating yeast after saccharification of the malt. Especially, as a malt extract, the extract of non-fermented malt is preferable.
また、(A)穀物由来たんぱく質として、大豆由来たんぱく質を用いてもよい。例えば、市販品の大豆由来たんぱく質としてハイニュートAM、ハイニュートDC6、ハイニュートDH(以上、不二精油社製)等を挙げることができる。本発明のpH領域においてはハイニュートAMがより好ましい。大豆由来たんぱく質としては、当該たんぱく質中の(D)分子量5000以下のペプチドの割合が高いものが好ましく、具体的には、大豆由来たんぱく質中の(D)分子量5000以下のペプチドの割合が73〜90質量%、更に75〜87質量%、更に78〜86質量%、殊更に80〜86質量%であることが好ましい。 Moreover, you may use a soybean origin protein as (A) grain origin protein. Examples of commercially available soybean-derived proteins include High New AM, High New DC6, High New DH (manufactured by Fuji Seiyaku Co., Ltd.) and the like. High pH AM is more preferable in the pH range of the present invention. As the protein derived from soybean, one having a high ratio of (D) peptide having a molecular weight of 5000 or less in the protein is preferable, and specifically, the ratio of peptide having a molecular weight of (D) of 5000 or less in the protein derived from soybean is 73 to 90. It is preferable that the content is 75% by mass, 75-87% by mass, 78-86% by mass, particularly 80-86% by mass.
本発明においては、(A)穀物由来たんぱく質として、(A1)麦芽由来のたんぱく質と、(A2)麦芽以外の穀物由来のたんぱく質を含有することが好ましく、(A2)麦芽以外の穀物由来のたんぱく質としては、大豆由来たんぱく質が好ましい。その場合、容器詰発泡飲料中の成分(A1)と(A2)との質量比[(A1)/(A2)]は、固形分換算として0.5〜100、更に0.8〜50、更に0.9〜20、殊更に1〜14が好ましい。 In the present invention, (A) a protein derived from a cereal preferably contains (A1) a protein derived from malt and (A2) a protein derived from a cereal other than malt, and (A2) a protein derived from a cereal other than malt. Is preferably a protein derived from soybeans. In that case, the mass ratio [(A1) / (A2)] of the components (A1) and (A2) in the packaged sparkling beverage is 0.5 to 100, further 0.8 to 50, and more preferably 0.5 to 50 in terms of solid content. 0.9-20, especially 1-14 are preferred.
また、本発明の容器詰発泡飲料は、酸味感の緩和及び喉越しの点から、成分(D)を飲料100g中に100〜3000mg、更に150〜2000mg、更に200〜1500mg含有することが好ましい。 Moreover, it is preferable that the container-packed sparkling beverage of this invention contains 100-3000 mg, Furthermore, 150-2000 mg, Furthermore, 200-1500 mg of component (D) in 100g of drinks from the point of relaxation of a sour taste and a throat.
本発明の容器詰発泡飲料は、成分(B)としてカリウムを含有する。(B)カリウムは、主に(A)穀物由来たんぱく質に由来するものであるが、新たに加えられたものでもよい。
本発明の容器詰発泡飲料中の(B)カリウムの含有量は、酸味感の緩和及び喉越しの観点から、飲料100g中に5mg以上、更に7mg以上、更に9mg以上であることが好ましく、他方上限は飲料100g中に40mg、更に30mg、更に20mg、更に15mgであることが好ましい。かかる(B)カリウムの含有量の範囲としては、5〜40mg、更に5〜30mg、更に7〜30mg、更に9〜20mg、殊更に9〜15mgが好ましい。なお、カリウム含有量の測定方法は、後掲の実施例に記載の方法にしたがうものとする。
The container-packed sparkling beverage of the present invention contains potassium as the component (B). (B) Potassium is mainly derived from (A) grain-derived protein, but may be newly added.
The content of (B) potassium in the packaged sparkling beverage of the present invention is preferably 5 mg or more, further 7 mg or more, and further 9 mg or more in 100 g of beverage, from the viewpoint of alleviation of sourness and over the throat, The upper limit is preferably 40 mg, further 30 mg, further 20 mg, and further 15 mg per 100 g of beverage. The range of the content of potassium (B) is preferably 5 to 40 mg, further 5 to 30 mg, further 7 to 30 mg, further 9 to 20 mg, and particularly 9 to 15 mg. In addition, the measuring method of potassium content shall follow the method as described in an Example mentioned later.
本発明の容器詰発泡飲料中の(D)分子量5000以下のペプチドと(B)カリウムとの質量比[(D)/(B)]は15〜300であるが、酸味感の緩和、喉越し及び清涼感の点から、下限は18、更に20、更に44、更に50、更に60が好ましく、他方上限は250、更に230、更に215、更に200が好ましい。かかる質量比[(D)/(B)]の範囲としては、18〜250、更に20〜230、更に44〜230、更に50〜215、殊更に60〜200が好ましい。 The mass ratio [(D) / (B)] of (D) a peptide having a molecular weight of 5000 or less and (B) potassium in the container-packed sparkling beverage of the present invention is 15 to 300. And, from the viewpoint of refreshing feeling, the lower limit is preferably 18, 20, 44, 50, or 60, while the upper limit is 250, 230, 215, or 200. The range of the mass ratio [(D) / (B)] is preferably 18 to 250, more preferably 20 to 230, further 44 to 230, further 50 to 215, and particularly preferably 60 to 200.
本発明の容器詰発泡飲料は、成分(C)として起泡剤を含有する。
(C)起泡剤としては泡立ちを付与できるものであれば特に限定されないが、例えば、炭酸ガス、サポニン、グリセリン脂肪酸エステル、プロピレングリコール脂肪酸エステル、ショ糖脂肪酸エステル等が挙げられる。これらは1種又は2種以上を組み合わせて使用することができる。中でも、泡の安定性やキメの細かさの点から、炭酸ガス、サポニンが好ましい。なお、サポニンとしては、トリテルペンやステロイドに糖が結合した配糖体であれば特に限定されないが、例えば、バラ科キラヤ(Quillaja saponaria MOLINA)の樹皮より抽出されたキラヤサポニンが好ましい。キラヤサポニンは、商業的に入手することが可能であり、例えば、キラヤニンC−100(商品名、丸善製薬株式会社製)が挙げられる。
The container-packed sparkling beverage of the present invention contains a foaming agent as component (C).
(C) The foaming agent is not particularly limited as long as it can impart foaming, and examples thereof include carbon dioxide, saponin, glycerin fatty acid ester, propylene glycol fatty acid ester, and sucrose fatty acid ester. These can be used alone or in combination of two or more. Of these, carbon dioxide and saponin are preferred from the viewpoint of foam stability and fineness of texture. The saponin is not particularly limited as long as it is a glycoside in which sugar is bound to triterpene or steroid, but for example, quillaja saponin extracted from the bark of Quillaja saponaria MOLINA is preferable. Kirayasaponin can be obtained commercially, for example, Kirayanin C-100 (trade name, manufactured by Maruzen Pharmaceutical Co., Ltd.).
起泡剤として炭酸ガスを使用する場合、圧入する炭酸ガスは、キメの細かい均質な泡の形成の観点から、ガス容量比で0.5〜4、更に1〜3.5、更に2〜3であることが好ましい。ここで、本明細書において「ガス容量比(ガスボリューム)」とは、飲料中に溶解している炭酸ガスの20℃における気体容量の飲料容量に対する比率をいう。
一方、炭酸ガス以外の起泡剤を使用する場合、本発明の容器詰発泡飲料中に0.001〜0.2質量%、更に0.005〜0.18質量%、更に0.01〜0.15質量%含有することが好ましい。
本発明においては、炭酸ガスと、炭酸ガス以外の起泡剤を組み合わせて用いることが可能であり、その場合の炭酸ガスと、炭酸ガス以外の起泡剤の各使用量は上記範囲内とすることが好ましい。
When carbon dioxide gas is used as the foaming agent, the carbon dioxide gas to be injected is from 0.5 to 4, more preferably from 1 to 3.5, and further from 2 to 3 in terms of gas volume ratio, from the viewpoint of forming fine and uniform foam. It is preferable that Here, in this specification, "gas volume ratio (gas volume)" means the ratio of the gas volume at 20 ° C of the carbon dioxide dissolved in the beverage to the beverage volume.
On the other hand, when using a foaming agent other than carbon dioxide, 0.001 to 0.2% by mass, further 0.005 to 0.18% by mass, and further 0.01 to 0% in the container-packed sparkling beverage of the present invention. It is preferable to contain 15 mass%.
In the present invention, carbon dioxide gas and a foaming agent other than carbon dioxide can be used in combination, and the amounts of carbon dioxide and foaming agents other than carbon dioxide in that case are within the above ranges. It is preferable.
本発明の容器詰発泡飲料は、アルコール飲料でも、ノンアルコール飲料であってもよい。ここで、本明細書において「ノンアルコール飲料」とは、飲料中の(E)アルコールの含有量が1質量%未満の飲料をいうが、本発明においては0.5質量%未満であることが、喉への刺激が少ない点から好ましい。なお、アルコール飲料の場合、飲料中の(E)アルコールの含有量は適宜選択可能であるが、例えば、1〜8質量%、更に2〜6質量%であることが好ましい。 The packaged sparkling beverage of the present invention may be an alcoholic beverage or a non-alcoholic beverage. Here, in the present specification, the “non-alcoholic beverage” refers to a beverage whose content of (E) alcohol in the beverage is less than 1% by mass, and in the present invention, it may be less than 0.5% by mass. From the viewpoint of less irritation to the throat. In the case of an alcoholic beverage, the content of the (E) alcohol in the beverage can be appropriately selected, but is preferably 1 to 8% by mass, and more preferably 2 to 6% by mass.
本発明の容器詰発泡飲料は、更に成分(F)として非重合体カテキン類を含有することができる。非重合体カテキン類の苦味により、喉を通過するときの酸味感を緩和することができ、また非重合体カテキン類による生理効果を期待することができる。ここで、本明細書において「非重合体カテキン類」とは、カテキン、ガロカテキン、カテキンガレート及びガロカテキンガレート等の非エピ体カテキン類と、エピカテキン、エピガロカテキン、エピカテキンガレート及びエピガロカテキンガレート等のエピ体カテキン類を併せての総称であり、非重合体カテキン類の濃度は、上記8種の合計量に基づいて定義される。なお、非重合体カテキン類含有量の測定方法は、後掲の実施例に記載の方法にしたがうものとする。
本発明の容器詰発泡飲料中の(F)非重合体カテキン類の含有量は、酸味感の緩和及び生理効果の観点から、0.001〜0.3質量%、更に0.005〜0.25質量%、更に0.01〜0.2質量%であることが好ましい。
The container-packed sparkling beverage of the present invention can further contain non-polymer catechins as the component (F). The bitter taste of non-polymer catechins can alleviate the sour taste when passing through the throat, and the physiological effect of non-polymer catechins can be expected. Here, in the present specification, “non-polymer catechins” refers to non-epimeric catechins such as catechin, gallocatechin, catechin gallate and gallocatechin gallate, epicatechin, epigallocatechin, epicatechin gallate and epigallocatechin. It is a collective term for epi-catechins such as gallate, and the concentration of non-polymer catechins is defined based on the total amount of the above eight types. In addition, the measuring method of non-polymer catechin content shall follow the method as described in an Example mentioned later.
The content of (F) non-polymer catechins in the packaged sparkling beverage of the present invention is 0.001 to 0.3% by mass, and further 0.005 to 0.005% from the viewpoints of mitigation of sourness and physiological effects. It is preferable that it is 25 mass%, and also 0.01-0.2 mass%.
更に、本発明の容器詰発泡飲料には、所望により、酸味料、甘味料、ビタミン、ミネラル、酸化防止剤、泡安定剤、各種エステル類、色素類、乳化剤、保存料、調味料、野菜エキス類、花蜜エキス類、品質安定剤等の添加剤を単独で又は2種以上を組み合わせて含有させることができる。なお、添加剤の含有量は、本発明の目的を損なわない範囲内で適宜設定可能である。 Furthermore, the container-packed sparkling beverage of the present invention may contain acidulants, sweeteners, vitamins, minerals, antioxidants, foam stabilizers, various esters, pigments, emulsifiers, preservatives, seasonings, vegetable extracts as desired. , Nectar extract, and a quality stabilizer can be contained alone or in combination of two or more. In addition, content of an additive can be suitably set within the range which does not impair the objective of this invention.
本発明の容器詰発泡飲料は、pH(20℃)が3〜5であるが、2.5〜4.5、更に3〜4であることが、酸味感の緩和、喉越し及び清涼感の点から好ましい。なお、pH調整には、(G)酸味料を使用することが可能であり、具体的には、リンゴ酸、クエン酸、乳酸等の有機酸、リン酸等の無機酸が挙げられる。これらは単独で又は2種以上を組み合わせて使用することができる。 The container-packed sparkling beverage of the present invention has a pH (20 ° C.) of 3 to 5, but 2.5 to 4.5, and further 3 to 4 has a soothing feeling, a throat feeling and a refreshing feeling. It is preferable from the point. In addition, it is possible to use (G) acidulant for pH adjustment, Specifically, inorganic acids, such as organic acids, such as malic acid, a citric acid, and lactic acid, and phosphoric acid, are mentioned. These can be used alone or in combination of two or more.
本発明の容器詰発泡飲料に使用できる容器としては、例えば、ポリエチレンテレフタレートを主成分とする成形容器(いわゆるPETボトル)、金属缶、瓶等の通常の包装容器が例示される。 Examples of containers that can be used in the container-packed sparkling beverage of the present invention include ordinary packaging containers such as molded containers (so-called PET bottles), metal cans, and bottles mainly composed of polyethylene terephthalate.
このような容器詰発泡飲料は、例えば、(D)分子量5000以下のペプチドの割合が70〜100質量%である(A)穀物由来たんぱく質と、必要により(E)アルコール及び添加剤を配合し、(D)分子量5000以下のペプチドと(B)カリウムの質量比、必要により(E)アルコール濃度が上記範囲内となるように調整して、これをPETボトル等の容器に注入し、次いで炭酸水を充填するポストミックス方式により製造することができる。また、例えば、金属缶のような容器に飲料を充填後、加熱殺菌できる場合にあっては適用されるべき法規(日本にあっては食品衛生法)に定められた殺菌条件で殺菌処理してもよい。一方、PETボトル、紙容器のようにレトルト殺菌できないものについては、あらかじめ上記と同等の殺菌条件、例えばプレート式熱交換器などで高温短時間殺菌後、一定の温度迄冷却して容器に充填する等の方法を採用することができる。 Such a container-packed sparkling beverage includes, for example, (D) a protein having a molecular weight of 5000 or less and a proportion of 70 to 100% by mass, (A) a protein derived from cereal, and (E) alcohol and additives as necessary. (D) The mass ratio of a peptide having a molecular weight of 5000 or less and (B) potassium, and if necessary, (E) the alcohol concentration is adjusted to be within the above range, and this is injected into a container such as a PET bottle, and then carbonated water Can be manufactured by a postmix method of filling In addition, for example, if a beverage such as a metal can is filled with a beverage and then heat sterilized, it should be sterilized under the sterilization conditions stipulated in the applicable regulations (Food Sanitation Law in Japan). Also good. On the other hand, those that cannot be sterilized by retort, such as PET bottles and paper containers, are sterilized under the same conditions as described above, for example, sterilized at high temperature and short time in a plate heat exchanger, and then cooled to a certain temperature and filled into containers. Etc. can be adopted.
また、本発明の容器詰発泡飲料の態様において、非重合体カテキン類を含有させる場合には、更にカテキン製剤を配合して(F)非重合体カテキン類濃度を上記範囲内となるように調整し、上記と同様の操作により製造することができる。この場合に使用するカテキン製剤としては、例えば、不発酵茶、半発酵茶及び発酵茶から選択される茶葉から熱水又は水溶性有機溶媒を用いてニーダー抽出やカラム抽出等により得られる抽出液が挙げられる。また、当該抽出液から溶媒の一部を除去して非重合体カテキン類濃度を高めた濃縮物を用いてもよい。カテキン製剤の形態としては、固体、水溶液、スラリー状等の種々のものが挙げられる。カテキン製剤として市販品を使用してもよく、例えば、三井農林(株)の「ポリフェノン」、伊藤園(株)の「テアフラン」、太陽化学(株)の「サンフェノン」等が挙げられる。 In the embodiment of the container-packed sparkling beverage of the present invention, when a non-polymer catechin is contained, a catechin preparation is further blended to adjust the concentration of the non-polymer catechin to fall within the above range. However, it can be produced by the same operation as described above. Examples of the catechin preparation used in this case include an extract obtained by kneader extraction or column extraction from tea leaves selected from non-fermented tea, semi-fermented tea and fermented tea using hot water or a water-soluble organic solvent. Can be mentioned. Moreover, you may use the concentrate which removed a part of solvent from the said extract, and raised non-polymer catechin density | concentration. The form of the catechin preparation includes various forms such as a solid, an aqueous solution, and a slurry. Commercial products may be used as the catechin preparation, and examples thereof include “Polyphenone” from Mitsui Norin Co., Ltd., “Theafuran” from ITO EN, “Sunphenon” from Taiyo Kagaku Co., Ltd., and the like.
また、カテキン製剤として、茶抽出液又はその濃縮物を精製して非重合体カテキン類の純度を高めた精製カテキン製剤を使用してもよい。精製方法としては、例えば、下記(i)〜(iv)のいずれかの方法、あるいは2以上の組み合わせが挙げられる。
(i)カテキン製剤を水、水溶性有機溶媒(例えば、エタノール)、又は水と水溶性有機溶媒との混合物(以下、「有機溶媒水溶液」という)に懸濁して生じた沈殿を除去した後、溶媒を留去する方法(例えば、特開2004−147508号公報、特開2004−149416号公報)。
(ii)カテキン製剤を、活性炭、酸性白土及び活性白土から選択される少なくとも1種の吸着剤と接触させる方法(例えば、特開2007−282568号公報)。
(iii)カテキン製剤を、合成吸着剤に吸着させた後、該合成吸着剤に有機溶媒水溶液を接触させて非重合体カテキン類を脱離させる方法(例えば、特開2006−160656号公報)。
(iv)カテキン製剤を、合成吸着剤に吸着させた後、該合成吸着剤に有機溶媒水溶液又は塩基性水溶液(例えば、水酸化ナトリウム水溶液)を接触させて非重合体カテキン類を脱離させ、次いで得られた脱離液を活性炭と接触させる方法(例えば、特開2008−079609号公報)。
Moreover, you may use the purified catechin formulation which refine | purified the tea extract or its concentrate, and raised the purity of non-polymer catechins as a catechin formulation. Examples of the purification method include any of the following methods (i) to (iv), or a combination of two or more.
(I) After removing the precipitate formed by suspending the catechin preparation in water, a water-soluble organic solvent (for example, ethanol), or a mixture of water and a water-soluble organic solvent (hereinafter referred to as “organic solvent aqueous solution”), A method of distilling off the solvent (for example, JP-A Nos. 2004-147508 and 2004-149416).
(Ii) A method of bringing a catechin preparation into contact with at least one adsorbent selected from activated carbon, acidic clay and activated clay (for example, JP-A-2007-282568).
(Iii) A method in which a catechin preparation is adsorbed on a synthetic adsorbent and then an organic solvent aqueous solution is brought into contact with the synthetic adsorbent to desorb non-polymer catechins (for example, JP-A-2006-160656).
(Iv) After the catechin preparation is adsorbed on the synthetic adsorbent, the synthetic adsorbent is contacted with an organic solvent aqueous solution or a basic aqueous solution (eg, sodium hydroxide aqueous solution) to desorb non-polymer catechins, Next, a method of bringing the obtained desorbing liquid into contact with activated carbon (for example, JP 2008-079609).
上記(i)〜(iv)の方法においては、カテキン製剤としてタンナーゼ処理したものを使用してもよい。ここで、本明細書において「タンナーゼ処理」とは、カテキン製剤にタンナーゼ活性を有する酵素を接触させることをいう。これにより、非重合体カテキン類中のガレート体率を調整することができる。なお、タンナーゼ処理における具体的な操作方法は公知の方法を採用することが可能であり、例えば、特開2004−321105号公報に記載の方法が挙げられる。 In the above methods (i) to (iv), a catechin preparation that has been tannase-treated may be used. As used herein, “tannase treatment” refers to bringing an enzyme having tannase activity into contact with a catechin preparation. Thereby, the gallate body rate in non-polymer catechins can be adjusted. In addition, the specific operation method in a tannase process can employ | adopt a well-known method, For example, the method as described in Unexamined-Japanese-Patent No. 2004-321105 is mentioned.
1.ペプチドの分子量の測定
1)標準溶液の調製
表1に示す分子量標準品それぞれについて、下記2)に示した方法で試験溶液を調製し、下記3)に示した高速液体クロマトグラフ操作条件により測定し、次の関係式を得た。
1. Measurement of peptide molecular weight 1) Preparation of standard solution For each of the molecular weight standard products shown in Table 1, test solutions were prepared by the method shown in 2) below, and measured according to the high performance liquid chromatograph operating conditions shown in 3) below. The following relational expression was obtained.
分子量M及び溶出時間Tの関係式(検量線):
logM=−0.3100429T+7.6232997
Relational expression of molecular weight M and elution time T (calibration curve):
logM = −0.3100429T + 7.6232997
2)試験溶液の調製
検体0.5gを採取し、これに水、アセトニトリル及びトリフルオロ酢酸の混合液(55:45:0.1)10mLを加えた。室温で一晩放置した後、孔径0.45μmのメンブレンフィルタで濾過し、得られた液を試験溶液とした。
2) Preparation of test solution 0.5 g of a sample was collected, and 10 mL of a mixed solution of water, acetonitrile and trifluoroacetic acid (55: 45: 0.1) was added thereto. After leaving overnight at room temperature, the solution was filtered through a membrane filter having a pore size of 0.45 μm, and the resulting solution was used as a test solution.
3)高速液体クロマトグラフ操作条件
機種 :Shodex GPC-101(昭和電工社製)
検出器 :紫外分光光度計 UV-41(昭和電工社製)
カラム :TSKgel G2500PWX1,φ7.8mm×300mm(東ソー社製)
カラム温度:40℃
移動相 :水、アセトニトリル及びトリフルオロ酢酸の混合液(55:45:0.1)
流量 :0.5mL/min
測定波長:220nm
注入量 :20μL
3) High-performance liquid chromatograph operating conditions Model: Shodex GPC-101 (manufactured by Showa Denko)
Detector: UV spectrophotometer UV-41 (Showa Denko)
Column: TSKgel G2500PW X1 , φ7.8mm × 300mm (manufactured by Tosoh Corporation)
Column temperature: 40 ° C
Mobile phase: Mixture of water, acetonitrile and trifluoroacetic acid (55: 45: 0.1)
Flow rate: 0.5 mL / min
Measurement wavelength: 220 nm
Injection volume: 20 μL
4)穀物由来たんぱく質に占める分子量5000以下のペプチドの含有割合の算出
上記の方法で測定して得られたクロマトグラムにおいて、分子量5000に相当する溶出時間を基に、全ピーク面積に対する分子量5000以下のピーク面積の比率を求めた。その比率と、下記6)に示した方法で求めた穀物抽出物中の含窒素化合物の全質量と、下記7)に示した方法でサンプルを調製し、アミノ酸自動分析法により求めた遊離アミノ酸の全質量とから、下記式(1)により、分子量5000以下のペプチドの含有割合を算出した。
4) Calculation of content ratio of peptide with molecular weight of 5000 or less in protein derived from cereal In the chromatogram obtained by measurement by the above method, based on the elution time corresponding to molecular weight 5000, molecular weight of 5000 or less with respect to the total peak area. The peak area ratio was determined. The ratio, the total mass of nitrogen-containing compounds in the cereal extract obtained by the method shown in 6) below, and a sample prepared by the method shown in 7) below, and the free amino acid obtained by automatic amino acid analysis From the total mass, the content ratio of peptides having a molecular weight of 5000 or less was calculated by the following formula (1).
穀物由来のたんぱく質に占める分子量5000以下のペプチドの含有割合((D)/(A)×100)=
[(全ピーク面積に占める分子量5000以下のピーク面積比率)×(穀物抽出物中の含窒素化合物の全質量)−(遊離アミノ酸の全質量)]/[(穀物抽出物中の含窒素化合物の全質量)−(遊離アミノ酸の全質量)]×100
Content ratio of peptide having molecular weight of 5000 or less in protein derived from cereal ((D) / (A) × 100) =
[(Peak area ratio of molecular weight of 5000 or less in the total peak area) × (total mass of nitrogen-containing compounds in cereal extract) − (total mass of free amino acids)] / [(of nitrogen-containing compounds in cereal extract) Total mass)-(total mass of free amino acids)] × 100
5)分子量5000以下のペプチドとカリウムとの含有割合の算出
分子量5000以下のペプチド/カリウム=
[(全ピーク面積に占める分子量5000以下のピーク面積比率)×(穀物由来たんぱく質の全質量)−(遊離アミノ酸の全質量)]/カリウム量
5) Calculation of content ratio of peptide having a molecular weight of 5000 or less and potassium Peptide / potassium having a molecular weight of 5000 or less =
[(Peak area ratio of molecular weight of 5000 or less in the total peak area) × (total mass of protein derived from grain) − (total mass of free amino acid)] / potassium amount
6)穀物抽出物中の含窒素化合物の測定
「三訂 早わかり栄養表示基準」(新開発食品保健研究会監修 中央法規出版(株))の132〜134頁に記載の「1 たんぱく質 (1)窒素定量換算法」に基づき測定した。このとき「計算(133頁右欄第9〜15行)」において使用する「窒素・たんぱく質換算係数」は6.25とした。
6) Measurement of nitrogen-containing compounds in cereal extracts “Three amendments to Nutrition Labeling Standards” (supervised by the Newly-Developed Food Health Study Group, Central Law Publishing Co., Ltd.), pages 132 to 134 “1 Protein (1) Nitrogen” Measured based on “quantitative conversion method”. At this time, the “nitrogen / protein conversion coefficient” used in “Calculation (page 133, right column, lines 9 to 15)” was 6.25.
7)遊離アミノ酸測定用サンプルの調製
遊離アミノ酸は、次に示す条件に従い、アミノ酸自動分析法にて測定した。
<測定試料の調製>
試料3gに、10w/v%のスルホサリチル酸溶液12.5mLを添加し、混和して抽出した。次いで、3mol/Lの水酸化ナトリウム水溶液にてpHを2.2に調整し、クエン酸ナトリウム緩衝液で25mLに定容し、濾過して試験溶液とし、次に示す装置及び測定条件にて分析を行った。
<装置及び操作条件>
機種:JLC−500/V(日本電子(株))
カラム:LCR−6 径4mm×長さ120mm(日本電子(株))
移動相:クエン酸リチウム緩衝液 P−12〜15、P−21(日本電子(株))
反応液:日本電子用ニンヒドリン発色溶液キット−II(和光純薬工業(株))
流量:移動相 0.50mL/min.、反応液 0.30mL/min.
測定波長:570nm(プロリン以外)、440nm(プロリン)
7) Preparation of Free Amino Acid Measurement Sample Free amino acids were measured by an amino acid automatic analysis method according to the following conditions.
<Preparation of measurement sample>
To 3 g of the sample, 12.5 mL of a 10 w / v% sulfosalicylic acid solution was added, mixed and extracted. Next, adjust the pH to 2.2 with a 3 mol / L aqueous sodium hydroxide solution, adjust the volume to 25 mL with a sodium citrate buffer, filter to obtain a test solution, and analyze it using the following equipment and measurement conditions. Went.
<Apparatus and operating conditions>
Model: JLC-500 / V (JEOL Ltd.)
Column: LCR-6 Diameter 4 mm x Length 120 mm (JEOL Ltd.)
Mobile phase: Lithium citrate buffer P-12-15, P-21 (JEOL Ltd.)
Reaction solution: ninhydrin coloring solution kit-II for JEOL (Wako Pure Chemical Industries, Ltd.)
Flow rate: Mobile phase 0.50 mL / min. , Reaction solution 0.30 mL / min.
Measurement wavelength: 570 nm (other than proline), 440 nm (proline)
2.カリウムの測定
カリウムは、「分析実務者が書いた五訂日本食品標準成分表分析マニュアルの解説」(財団法人日本食品分析センター編集、中央法規出版株式会社発行所、2001年7月10日発行)のp.90−91及びp.99−103にしたがい測定した。具体的には、検体2〜5gを抽出容器に量り取り、1%塩酸溶液200mLを加え、室温下で30分振とう抽出した。抽出液を遠心管に移し、遠心分離後の上澄み液を原子吸光用試験溶液とした。原子吸光光度計の測定波長を766.5nmに設定し、カリウムを測定した。そして、予め作成した検量線を用いて試料中のカリウム量を定量した。
2. Measurement of Potassium Potassium is “Explanation of Analysis Manual for Five Standards of Japanese Food Standards Written by Analyst Practitioners” (edited by the Japan Food Analysis Center, issued by Chuo Law Publishing Co., Ltd., issued July 10, 2001) P. 90-91 and p. Measured according to 99-103. Specifically, 2 to 5 g of the sample was weighed in an extraction container, 200 mL of 1% hydrochloric acid solution was added, and the mixture was extracted by shaking at room temperature for 30 minutes. The extract was transferred to a centrifuge tube, and the supernatant after centrifugation was used as a test solution for atomic absorption. The measurement wavelength of the atomic absorption photometer was set to 766.5 nm, and potassium was measured. And the amount of potassium in a sample was quantified using the calibration curve prepared beforehand.
3.非重合体カテキン類の測定
検体をフィルター(0.45μm)で濾過し、高速液体クロマトグラフ(型式SCL−10AVP、島津製作所製)を用い、オクタデシル基導入液体クロマトグラフ用パックドカラムL−カラムTM ODS(4.6mmφ×250mm:財団法人 化学物質評価研究機構製)を装着し、カラム温度35℃でグラジエント法により分析した。移動相A液は酢酸を0.1mol/L含有する蒸留水溶液、移動相B液は酢酸を0.1mol/L含有するアセトニトリル溶液とし、試料注入量は20μL、UV検出器波長は280nmの条件で行った。
3. Measurement of non-polymer catechins Samples are filtered through a filter (0.45 μm), and a high-performance liquid chromatograph (model SCL-10AVP, manufactured by Shimadzu Corporation) is used to pack an octadecyl group-introduced packed column L-column TM ODS. (4.6 mmφ × 250 mm: manufactured by Chemical Substance Evaluation Research Organization) was attached, and analysis was performed at a column temperature of 35 ° C. by a gradient method. The mobile phase A solution is a distilled aqueous solution containing 0.1 mol / L of acetic acid, the mobile phase B solution is an acetonitrile solution containing 0.1 mol / L of acetic acid, the sample injection volume is 20 μL, and the UV detector wavelength is 280 nm. went.
4.官能評価
1)酸味感
各実施例及び比較例で得られた容器詰発泡飲料について、飲用時の酸味感が緩和されたか否かを評価した。評価は、3名の男性モニターに、1回につき100mLを飲用してもらい、以下の基準により評点をつけさせた。その後、協議により最終スコアを決定した。なお、発泡飲料は、冷蔵所にて5℃で10日間保管したものを試験に用いた。
4). Sensory evaluation 1) sourness About the container-packed sparkling beverages obtained in each Example and Comparative Example, it was evaluated whether or not the sourness during drinking was alleviated. For evaluation, three male monitors were allowed to drink 100 mL at a time, and scored according to the following criteria. After that, the final score was determined by consultation. In addition, the sparkling beverage used for the test what was stored for 10 days at 5 degreeC in the refrigerator.
4:酸味感が気にならない
3:酸味感が気になりにくい
2:酸味感がやや気になる
1:酸味感が気になる
4: I don't care about sourness 3: I don't care about sourness 2: I'm worried about sourness 1: I'm worried about sourness
2)喉越し
各実施例及び比較例で得られた容器詰発泡飲料の汗をかいて飲むときの喉越しについて評価した。評価は、3名の男性モニターに約10分間のジョギングし、汗をかいたときに1回につき100mLを飲用してもらい、以下の基準で評点をつけさせた。その後、協議により最終スコアを決定した。なお、発泡飲料は、冷蔵所にて5℃で10日間保管したものを試験に用いた。
2) Over the throat The over-the-throat when the container-packed sparkling beverages obtained in each Example and Comparative Example were sweated was evaluated. Evaluation was performed by jogging for about 10 minutes on three male monitors and drinking 100 mL at a time when sweating. After that, the final score was determined by consultation. In addition, the sparkling beverage used for the test what was stored for 10 days at 5 degreeC in the refrigerator.
4:喉越しが良い
3:喉越しがやや良い
2:喉越しがやや良くない
1:喉越しが良くない
4: Good over the throat 3: A little over the throat 2: A little over the throat 1: A little over the throat 1: Not good over the throat
3)外観評価
各実施例及び比較例で得られた容器詰発泡飲料を、5℃にて10日間静置して保管したときの外観について目視で観察し、以下の基準で評点をつけさせた。
4:濁り・沈殿がほとんどない
3:濁り・沈殿がややある
2:濁り・沈殿が多い
1:濁り・沈澱がかなり多い
3) Appearance evaluation The appearance when the container-packed sparkling beverages obtained in each of the Examples and Comparative Examples were stored at 5 ° C. for 10 days was visually observed and scored according to the following criteria. .
4: There is almost no turbidity / precipitation 3: There is a little turbidity / precipitation 2: Many turbidity / precipitation 1: There is much turbidity / precipitation
〔ペプチド製剤の製造〕
1)麦汁の乾燥物
麦芽600gを1000mLの水に入れ、45℃に加温し3時間攪拌した。得られた抽出液を2号濾紙にて濾過することにより麦芽の殻を分離し、濾液を凍結乾燥して麦汁の乾燥物を得た。得られた麦汁の乾燥物中の全たんぱく質含有量は6.3質量%、遊離アミノ酸含有量は1.3質量%、全たんぱく質に占める分子量5000以下のペプチドの割合は74質量%であった。
[Production of peptide preparation]
1) Dry matter of wort 600 g of malt was put in 1000 mL of water, heated to 45 ° C. and stirred for 3 hours. The resulting extract was filtered through No. 2 filter paper to separate the malt shell, and the filtrate was freeze-dried to obtain a dried wort. The total protein content in the obtained dried wort was 6.3% by mass, the free amino acid content was 1.3% by mass, and the proportion of peptides with a molecular weight of 5000 or less in the total protein was 74% by mass. .
2)市販のペプチド製剤
・ペプチドW:大豆由来、ハイニュートAM(不二製油(株))全たんぱく質に占める分子量5000以下のペプチドの割合85質量%。遊離アミノ酸含有量2.6質量%。
・ペプチドX:大豆由来、ハイニュートDC6(不二製油(株))全たんぱく質に占める分子量5000以下のペプチドの割合72質量%。遊離アミノ酸含有量1.7質量%。
・ペプチドY:大豆由来、ハイニュートDH(不二製油(株))全たんぱく質に占める分子量5000以下のペプチドの割合18質量%。遊離アミノ酸含有量0.1質量%。
・ペプチドZ:豚由来、スーパーコラーゲンペプチドSCPシリーズ(新田ゼラチン社)全たんぱく質に占める分子量5000以下のペプチドの割合95質量%。遊離アミノ酸含有量0.1質量%。
2) Commercially available peptide preparation / Peptide W: 85% by mass of a peptide having a molecular weight of 5000 or less in the total protein derived from soybean, high-new AM (Fuji Oil Co., Ltd.). Free amino acid content 2.6 mass%.
Peptide X: A soybean-derived, high-newt DC6 (Fuji Oil Co., Ltd.) 72% by mass of peptide having a molecular weight of 5000 or less in the total protein. Free amino acid content 1.7% by mass.
Peptide Y: 18% by mass of a peptide having a molecular weight of 5000 or less in the total protein derived from soybean, high-new DH (Fuji Oil Co., Ltd.). Free amino acid content 0.1% by mass.
Peptide Z: 95% by mass of porcine-derived super collagen peptide SCP series (Nitta Gelatin Co., Ltd.) with a molecular weight of 5000 or less in the total protein. Free amino acid content 0.1% by mass.
〔精製緑茶抽出物の製造〕
緑茶抽出液の濃縮物(ポリフェノンHG、三井農林(株)製)200gを、25℃にて250r/min攪拌条件下において95質量%エタノール水溶液800g中に分散させ、酸性白土(ミズカエース#600、水澤化学(株)製)100gを投入後、約10分間攪拌を続けた。次に、2号ろ紙で濾過した後、濾液に活性炭16gを添加し、再び2号ろ紙で濾過した。次に0.2μmメンブランフィルターで再濾過した。次に、40℃、減圧下にて濾液からエタノールを留去し、イオン交換水で非重合体カテキン類濃度を調整して「精製緑茶抽出物」を得た。精製緑茶抽出物中の非重合体カテキン類濃度は15質量%であった。
[Production of purified green tea extract]
200 g of a green tea extract concentrate (Polyphenon HG, manufactured by Mitsui Norin Co., Ltd.) was dispersed in 800 g of a 95% by mass ethanol aqueous solution at 25 ° C. under a 250 r / min stirring condition, and acid clay (Mizuka Ace # 600, Mizusawa). After adding 100 g of Chemical Co., Ltd., stirring was continued for about 10 minutes. Next, after filtering with No. 2 filter paper, 16 g of activated carbon was added to the filtrate and filtered again with No. 2 filter paper. Next, it re-filtered with the 0.2 micrometer membrane filter. Next, ethanol was distilled off from the filtrate under reduced pressure at 40 ° C., and the concentration of non-polymer catechins was adjusted with ion-exchanged water to obtain a “purified green tea extract”. The concentration of non-polymer catechins in the purified green tea extract was 15% by mass.
実施例1
イオン交換水60gに麦汁の乾燥物2.1gとペプチドW0.15gを混合した後、pH3.8となるようにリン酸を添加し、イオン交換水で全量を120gにした。次に、この溶液120gに、5℃に冷却した炭酸水で全量を500gとし、炭酸ガスボリュームを2.5とし、耐圧性PETボトルに充填し巻き締め後、65℃で10分殺菌して容器詰発泡飲料(容器詰炭酸飲料)を調製した。得られた容器詰発泡飲料の分析結果及び官能試験の結果を表2に示す。
Example 1
After mixing 2.1 g of dried wort and 0.15 g of peptide W in 60 g of ion-exchanged water, phosphoric acid was added so that the pH was 3.8, and the total amount was adjusted to 120 g with ion-exchanged water. Next, 120 g of this solution is made up to 500 g with carbonated water cooled to 5 ° C., the carbon dioxide gas volume is set to 2.5, filled into a pressure-resistant PET bottle, wound and sterilized at 65 ° C. for 10 minutes. A packed sparkling beverage (a container-packed carbonated beverage) was prepared. Table 2 shows the analysis results and sensory test results of the resulting packaged sparkling beverage.
実施例2〜15及び比較例1〜7
表2に示す各成分を、表2に示す割合で配合したこと以外は、実施例1と同様の操作により、容器詰発泡飲料を得た。得られた容器詰発泡飲料の分析結果及び官能試験の結果を表2に示す。
Examples 2 to 15 and Comparative Examples 1 to 7
A container-packed sparkling beverage was obtained in the same manner as in Example 1 except that the components shown in Table 2 were blended in the proportions shown in Table 2. Table 2 shows the analysis results and sensory test results of the resulting packaged sparkling beverage.
表2から、容器詰発泡飲料において、(A)穀物由来たんぱく質に占める(D)分子量5000以下のペプチドが特定の割合である穀物由来のたんぱく質を用い、(D)分子量5000以下のペプチドと(B)カリウムとの質量比、及びpHを特定範囲内に制御することで、喉越しが良好でかつ酸味感が緩和されており、汗をかいて水分を欲するときに飲む止渇飲料として適した容器詰発泡飲料が得られることが確認された。 From Table 2, in a packaged sparkling beverage, (D) a protein derived from a grain having a specific ratio of (D) a peptide having a molecular weight of 5000 or less in (A) a protein derived from a grain, and (D) a peptide having a molecular weight of 5000 or less and (B ) By controlling the mass ratio with potassium and the pH within a specific range, the container is suitable for a dry drink that has a good feeling over the throat and relaxed sourness, and drinks when you sweat and want moisture. It was confirmed that a stuffed sparkling beverage was obtained.
Claims (5)
(A)穀物由来たんぱく質
(B)カリウム、及び
(C)起泡剤
を含有し、
前記成分(A)に占める(D)分子量5000以下のペプチドの割合が70〜100質量%であり、
前記成分(D)と前記成分(B)との含有質量比[(D)/(B)]が15〜300であり、
前記成分(B)の含有量が飲料100g中に5〜40mgであり、
前記成分(D)の含有量が飲料100g中に100〜3000mgであり、
(G)酸味料を使用してpHを調整することでpHが3〜4であり、
前記成分(A)として(A1)麦芽由来のタンパク質及び(A2)大豆由来のタンパク質を含有する、容器詰発泡飲料。 The following components (A), (B) and (C);
(A) a protein derived from cereal (B) potassium, and (C) a foaming agent,
The proportion of (D) peptides having a molecular weight of 5000 or less in the component (A) is 70 to 100% by mass,
The mass ratio [(D) / (B)] of the component (D) and the component (B) is 15 to 300,
Content of the said component (B) is 5-40 mg in 100g of drinks,
Content of the said component (D) is 100-3000 mg in 100g of drinks,
(G) pH is 3-4 by adjusting pH using acidulant,
A container-packed sparkling beverage containing (A1) malt-derived protein and (A2) soybean-derived protein as the component (A).
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