JP2014079208A - Supplement for improving intestinal environment and intestinal tract barrier - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a supplement for improving intestinal environment and intestinal tract barrier, which improves the weakened barrier function of intestinal tract and the damage to epithelial cells of intestinal tract and inhibits the antigen-antibody reaction due to undigested protein.SOLUTION: A supplement for improving intestinal environment and intestinal tract barrier for lowering intestinal environment barrier index includes a mixture of component (A) and component (B). The component (A) includes at least one of: probiotics including any one of lactic acid bacteria, bifidobacteria, saccharification bacteria, bacillus natto, and yeasts, or the mixture thereof; biogenics which produce bacteria such as lactic acid bacteria; and prebiotics which include at least a guar gum enzyme degradation product. The component (B) includes at least L-glutamine, zinc products, vitamin A products, and vitamin D products.

Description

  The present invention relates to an intestinal environment and intestinal barrier improving supplement for the purpose of improving intestinal fragility and intestinal allergy.

  For example, Patent Document 1 discloses one or more selected from vitamins, minerals, amino acids, peptides, proteins, carbohydrates, oligosaccharides, polysaccharides, lipids, nucleic acids, and other food materials having health maintenance / promotion effects. Nutritional supplements and health foods containing the ingredients for replenishing the ingredients are eaten simultaneously with the foods by adding the foods when eating existing foods Has been proposed.

  The nutritional supplements and health foods according to the invention described in Patent Document 1 take into account compatibility with existing foods to be added at the time of product design, and are intended to improve intestinal fragility and intestinal allergy It was not designed.

JP 2009-159873 A

  In this invention, a food material having a health maintenance / promotion effect is formulated so as to improve intestinal fragility and intestinal allergy, and an intestinal environment / intestinal barrier improving supplement that lowers the intestinal environment / barrier index is provided. The issue is to provide.

  In order to solve the above-described problems, the present invention provides a biogenic substance such as a lactic acid bacterium, a bifidobacteria, a saccharification bacterium, a natto bacterium, a yeast, or a probiotic that is a mixture thereof, or a microbial product such as lactic acid bacterium. Component (A) consisting of at least one of prebiotics comprising at least one of an enzyme degradation product and guar gum enzyme, L-glutamine, zinc, vitamin A (hereinafter referred to as VA), vitamin D (hereinafter referred to as VD) The present invention provides a supplement for improving the intestinal environment and intestinal tract barrier that lowers the intestinal environment / barrier index (explained later), by mixing at least one component (B).

  According to the intestinal environment and intestinal barrier improving supplement according to the present invention, the intestinal bacterium originally increases the suppression of harmful bacteria, nutrient production, promotion of intestinal peristalsis and activation of mucosal defense function, The index can be lowered (normalized) to improve intestinal fragility and intestinal allergy.

The top view which shows the display to the effect that the supplement which concerns on the Example of this invention is used in order to arrange the condition of the stomach Diagram showing comparison of intestinal environment / barrier index (IgG score) for dairy products, seafood, meat / eggs before and after intake of supplements according to Example of first subject Diagram showing comparison of intestinal environment and barrier index for similar vegetables, nuts / cereals and other foods Diagram showing comparison of intestinal environment / barrier index for vegetables, nuts / cereals and other foods before and after intake of supplements according to Example of the second subject Diagram showing comparison of intestinal environment and barrier index for similar dairy products, seafood, meat / eggs, fruits and spices

  Hereinafter, embodiments of the present invention will be described in detail.

  The supplement for improving intestinal environment and intestinal barrier according to the present invention comprises (A) component consisting of at least one of probiotics, biogenics, and prebiotics, L-glutamine, zincs, V. Class A, V. It is formed by mixing the component (B) consisting of at least one of the D classes.

  The above "probiotics" are "living microorganisms that give beneficial effects to host animals by improving the balance of intestinal microbial flora", and include lactic acid bacteria, bifidobacteria, natto bacteria, butyric acid bacteria, “Prebiotics” are “substances that act favorably on the health of the host by selectively acting on useful bacteria that live in the intestines and promoting their growth or their activity”. B. G. Indigestible food ingredients such as S (bifidobacterial production stimulating substance) are applicable, and “Biogenics” means “direct biological regulation, biological defense, disease prevention, recovery, aging control, etc. without going through intestinal flora” Food components such as CPP (casein phosphopeptide) which is a physiologically active peptide, lactoferrin which is a multifunctional whey protein, and DHA (docosahexaenoic acid) which is a kind of polyunsaturated fatty acid.

  In the present embodiment, the number of enteric bacteria called good bacteria is increased by at least one of probiotics, biogenics, and prebiotics so that the intestinal environment / barrier index decreases. By reducing the intestinal bacteria called bad bacteria, it promotes the suppression of harmful bacteria, nutrient production, promotion of intestinal peristalsis, activation of mucosal defense functions, and improvement of the intestinal environment.

Specifically, the control of harmful bacteria is based on the prevention of the establishment of harmful bacteria, the lowering of pH by organic acids (by lactic acid and acetic acid), and the production of antibacterial substances (bacteriocin). Further, the nutritional production, degradation of indigestible polysaccharides, vitamins production _{(V.B 5, V.H, V.K)} , and there in the production of short chain fatty acids (butyric acid) which is a nutritional intestinal epithelium The activation of mucosal defense function is an increase in production of secretory IgA and C-type secretin.

  Immunoglobulin G (IgG) accounts for over 70% of plasma antibodies (IgE is present only at 0.001% or less) and plays a role in antibody neutralization and antigen presentation in cellular immunity. Cellular immunity is called type IV allergy (late onset) and develops an allergic reaction over several hours to several days.

  IgG passes through the placenta and can save the newborn's immunity after birth. In addition, the presence time in the body is about 3 weeks.

  Considering the cause of food allergies, there is no food production since there are usually no food-derived proteins in the blood. That is, the absence of food antigen-specific IgG is normal. However, undigested protein has been absorbed due to the weakness of the intestinal barrier due to abnormalities in the intestinal environment and indigestion, so IgG for food protein is produced, and as a result, food antigen-specific IgG is produced in the blood. Detected.

  The intestinal environment / barrier index is a score of a food antigen-specific IgG value that induces late food allergies. “0” without detection is normal, and the higher the value, the greater the intestinal environment and intestinal tract. Any abnormality of the barrier is estimated.

  In addition, the present invention provides an intestinal environment by using the component (B) composed of at least one of probiotics, biogenics and prebiotics as described above and the following component (B). The barrier function of the intestinal tract is restored to improve allergy to food antigens, particularly late-type food allergy induced by IgG.

  The above-mentioned probiotics, biogenics, and prebiotics only need to use at least one of these as the component (A), and the component (B) used in combination with the component (A) includes L-glutamine, Zinc, V. Class A, V. It consists of at least one (1 or 2 or more) among D classes.

  Here, the zinc includes at least zinc among zinc and zinc gluconate, Class A is V.V. A, pre-V. A, V. Among the derivatives of A, at least V. A and V. Class D is V.V. D, pre-V. D, V.D. Among the derivatives of D, at least V. D may be included.

  Further, the following component (C) may be added to the component (A) and the component (B) and mixed. Component (C) is V. Group B; calcium; magnesium; magnesium compound; functional nutritional component consisting of at least one of quercetin, lactoferrin and curcumin; protein; at least one of amino acids.

  Specifically, spore-forming lactic acid bacteria may be used as probiotics. In addition, the prebiotic may contain at least a guar gum enzyme degradation product among a guar gum enzyme degradation product, isomaly oligosaccharide, fructooligosaccharide, and cyclic oligosaccharide which are water-soluble dietary fibers.

  Furthermore, in the component (B), the zincs include at least zinc among zinc and zinc gluconate. Class A is V.V. A, pre-V. A, V. Among the derivatives of A, at least V. A and V. Class D is V.V. D, pre-V. D, V.D. Among the derivatives of D, at least V. D should be included.

  In addition to the above calcium, magnesium, magnesium compound, etc., protein may be used as the component (C). The calcium includes shellfish calcium, the magnesium compound includes magnesium oxide, the protein includes a low-antigenic soybean peptide, and the amino acid may include L-methionine in order to improve the amino acid score of the soybean peptide. .

  Probiotics and / or prebiotics and (B) component zinc or zinc compound; Class A (especially VA) or V.A. When combined with at least one of class D (especially VD), these are essential nutrients for intestinal epithelial cells to undergo cell division and metabolism, and thus efficiently regenerate and restore intestinal epithelial cells. be able to. Moreover, when biogenics and (B) component are used together, an intestinal regulation effect and intestinal decay suppression can be performed.

  In particular, V.I. A or V. D is an essential nutrient for acting on an antigen-presenting cell responsible for an immune response, a helper T cell, and a B cell that produces an antibody in the vicinity of the intestinal epithelium to produce an optimal immune response. Suppresses the induction of adverse immune responses such as allergies and autoantibody production.

  Any of the above and the component (C), ie, V. When combined with functional nutrients such as group B, calcium, magnesium, or a compound thereof, quercetin, lactoferrin, curcumin, calcium, magnesium, or a compound thereof can control stool properties, and quercetin has an anti-inflammatory effect. In addition, when protein or amino acid necessary for protein synthesis is added, it is possible to enhance the efficient regeneration and restoration of intestinal epithelial cells and to add functionality.

  In recent years, abuse of antibiotics and anti-inflammatory analgesics has made the intestinal barrier function fragile. Intestinal tracts that are damaged by intestinal bacteria due to overeating, excessive drinking of alcohol, food contamination such as residual agricultural chemicals, and a decrease in the amount of dietary fiber intake, and fatty acids that are decomposed by intestinal bacteria as nutrients The epithelial cells of the intestinal tract are becoming fragile and the intestinal epithelial cells are damaged by the exotoxin produced by the increasing bad bacteria.

  As a result of the above, undigested protein reaches antigen-presenting cells near the intestine and induces an immune response against the food protein, resulting in various inflammations caused by local and systemic antigen-antibody reactions. Symptoms are present.

  For example, reflux esophagitis, obesity, edema, atopic dermatitis and the like. All of these are specific IgG-positive cases, and in all cases, by measuring IgG and combining the removal of highly responsive food with the administration of the supplement of the present invention that lowers the intestinal environment / barrier index, Reducing or radical healing can be obtained.

  Next, the amount contained in one recommended intake of the component (A) and the component (B) in the embodiment of the present invention will be described. In any case, the lower limit value indicates a numerical value that the effect cannot be expected if less than this. Moreover, an upper limit shows the numerical value by which an effect does not increase in principle even if it ingests once more than this.

  If the component (A) is, for example, a spore-forming lactic acid bacterium, the single dose exceeds 25 million.

  Zinc in the component (B) is preferably between 10 mg and 120 mg. When it exceeds 30 mg, copper shortage may occur due to excess zinc, so 5% or more of copper is added to zinc.

  When the main component of the component (B) is L-glutamine, 100 mg or more is included. 1000 mg to 5000 mg is desirable. When L-glutamine exceeds 1000 mg, stool water absorption increases and stool becomes stiff due to rapid improvement of colon function. As a result, since constipation or intestinal obstruction may occur, severely ill or elderly people need to take measures such as reducing the amount used or taking more water.

  Further, the main component of the component (B) is V.V. A, pre-V. In the case of A or a derivative thereof, V.V. A includes 100 IU to 60,000 IU.

  V. When A is less than 100 IU, efficient intestinal epithelial cell consolidation cannot be achieved, and the upper limit is V. This is to avoid A excess. If it exceeds 10,000 IU, it is necessary to be careful because it exceeds the recommended upper limit of intake during pregnancy.

  The main component of component (B) is V. D, pre-V. When D or a derivative thereof, V. D includes 100 IU to 10,000 IU.

  V. When D is less than 100 IU, the suppression of induction of adverse immune responses such as allergies and autoantibody production is insufficient. In addition, V.I. There is a risk of D excess.

As shown in FIG. 1, the intestinal environment and intestinal barrier improving supplement according to an embodiment of the present invention includes spore-forming lactic acid bacteria as probiotics, inulin, oligosaccharides and cyclic oligosaccharides as prebiotics (A ) Component, L-glutamine, zinc gluconate, β-carotene, V. A, V. Component (B) consisting of D. B ₁ , V. B ₂ , niacin (V.B ₃ ), V. B ₆ , V.I. B ₁₂ , vitamin B group consisting of folic acid; shellfish calcium and pantothenic acid (V.B ₅ ) Ca; oxidized Mg; soybean peptide; (C) component consisting of L-methionine; and polysaccharide used as a food additive A maltodextrin, V. C, V.D. E, biotin, potassium chloride, pectin as a stabilizer, and ferric pyrophosphate, which is iron chloride, are mixed and dissolved once in a package (30 g) in 80-180 ml of water. It is intended to be taken once or twice a day.

Ingredients per packet (30 g) were 14.9 g of protein (mainly soy peptide), 0.4 g of lipid, 9.4 g of carbohydrate (mainly oligosaccharide, cyclic oligosaccharide, inulin), 192 mg of sodium, V. A1000 IU, β-carotene 500 IU, V.I. B ₁ 10 mg, V.I. B ₂ 10 mg, V.I. B ₆ 15 mg, V.I. B ₁₂ 100 μg, pantothenic acid Ca 100 mg, folic acid 200 μg, biotin 100 μg, niacin 30 mg, C 100 mg, V.I. D800 IU, V.I. E20 mg, Ca 150 mg, Mg 150 mg, Cu 0.3 mg, Zn 15 mg, Fe 5 mg, Kcl 500 mg.

[Clinical case 1]
FIG. 2 and FIG. 3 show changes in IgG score for each food shown in the Japanese standard food panel before and after treatment of the 40-year-old woman of Clinical Example 1. Here, the IgG score indicates the severity of non-immediate allergy based on the amount of antibody. As for these levels (level 0 to IV), the level 0 is normal and the larger the level, the more abnormal the intestinal environment and the intestinal barrier are suggested. That is, the smaller the IgG level scored as the intestinal environment / barrier index, the closer to normal. The subjects were instructed not to ingest dairy products and eggs exceeding Level III at the first visit for 12 months.

  Fig. 2 shows the IgG scores for dairy products, seafood, meat and eggs before treatment, after 3 months and 12 months, and Fig. 3 shows the respective IgG scores for vegetables, nuts and grains, etc., before treatment and after 12 months. Show.

  In Clinical Example 1, as shown in FIG. 2, the IgG score of the dairy product was at level II or immediately before level II, but after 12 months, it all became level 0. The IgG score for seafood was slightly reduced for abalone and oysters, but all others were level 0. The IgG score for meat and eggs was slightly decreased for egg white and egg yolk, and the other meats were at level 0.

  In addition, as shown in FIG. 3, asparagus, bean sprout, kelp, tomato, soybean, buckwheat, wheat gluten, oats, peanuts, rye, sugar cane whose intestinal environment / barrier index before treatment was level II or III The foods for bread yeast were all greatly improved to level 0, and the levels of kidney beans and soybeans that were level IV to VI before treatment were reduced to I or II. Eggs did not stop, so there was no improvement.

[Clinical case 2]
Clinical example 2 relates to adult-onset atopic dermatitis in a 30-year-old male. FIG. 4 and FIG. 5 show the IgG levels at the first visit and after 6 months of treatment for each food in the standard food panel. Show. As in Clinical Example 1, subjects were instructed not to take dairy products and eggs whose intestinal environment / barrier index at the first visit exceeded Level III for 6 months.

  As shown in FIG. 4, asparagus, bean sprout, and kelp were all at level III at the first visit in the vegetables, but became level II or less after 6 months. As for nuts and cereals, soybeans and whole wheat, which had level IV and wheat gluten, peanuts, and rye at level III at the first visit, all decreased to level 0. In other foods, sugarcane, bakery yeast, and brewer's yeast at Level III or IV decreased from Level I to III at the first visit.

  In addition, as shown in FIG. 5, the dairy products all had IgG levels of IV, but decreased to level I or II, and the fruits had IgG levels of I for net melon, II were orange, watermelon Are all level 0. In seafood, abalone, oysters, and shrimp were reduced to level 0 or I in IgG level II or III, and egg yolk and egg white in level III or IV in meat were level 0 or I. In spices, it was level III, but Chile and vanilla were level 0.

  Although the supplement of the present invention contains 15 g of soy peptide per serving, even if it is ingested daily, the IgG against soybean decreases from level IV to level 0, indicating that the intestinal environment and the intestinal barrier are vulnerable. Supports the development of food-specific IgG allergies. This is in contrast to the egg case of Clinical Example 1 and reflects the characteristics that dairy products and eggs are more likely to form IgG allergies than soybeans.

  INDUSTRIAL APPLICABILITY The present invention can be used for a supplement that improves the intestinal environment and intestinal barrier of a human and reduces the occurrence of inflammation caused by an antigen-antibody reaction in the intestinal tract.

Claims (12)

Probiotics that are any of lactic acid bacteria, bifidobacteria, saccharified bacteria, natto bacteria, and yeasts, or a mixture thereof, biogenics that are bacterial products such as lactic acid bacteria, and at least prebiotics consisting of guar gum enzyme degradation products (A) component which consists of at least one of them,
(B) component consisting of at least one of L-glutamine, zinc, vitamin A, vitamin D;
An intestinal environment and intestinal barrier improving supplement that reduces the intestinal environment and barrier index. In claim 1,
The probiotic is a spore-forming lactic acid bacterium, and the prebiotic contains at least a guar gum enzyme degradation product among a water-soluble dietary fiber guar gum enzyme degradation product, isomaly oligosaccharide, fructooligosaccharide, and cyclic oligosaccharide. A supplement for improving intestinal environment and intestinal barrier. In claim 1 or 2,
The zinc includes at least zinc of zinc and zinc gluconate, the vitamin A includes vitamin A, previtamin A, and at least vitamin A among derivatives of vitamin A, the vitamin D includes vitamin D, A supplement for improving intestinal environment and intestinal barrier, characterized by containing at least vitamin D among previtamin D and derivatives of vitamin D. In any one of Claims 1 thru | or 3,
The component (A) and the component (B) include vitamin B group; calcium; magnesium; magnesium compound; functional nutritional component consisting of at least one of quercetin, lactoferrin and curcumin; protein; consisting of at least one of amino acids. (C) The intestinal environment and intestinal barrier improvement supplement which mixes an ingredient and reduces an intestinal environment and a barrier index. In claim 4,
The intestinal environment and intestinal barrier improving supplement, wherein the calcium contains shellfish calcium, the magnesium compound contains magnesium oxide, the protein contains soybean peptide, and the amino acid contains L methionine. In any one of Claims 1 thru | or 5,
When the component (A) is a spore-forming lactic acid bacterium, a supplement for improving the intestinal environment and intestinal tract barrier, wherein the number of spore-forming lactic acid bacteria contained in one recommended intake exceeds 25 million. In any one of Claims 1 thru | or 6.
A supplement for improving the intestinal environment and intestinal tract barrier, characterized in that when zinc is contained in the component (B), the weight of zinc in one recommended intake is 10 to 120 mg. In any one of Claims 1 thru | or 7,
When L-glutamine is contained in the component (B), L-glutamine contained in one recommended intake is 100 mg or more, and when L-glutamine is the main component, it is 1000 to 5000 mg Intestinal environment and intestinal barrier improvement supplement. In any one of Claims 1 thru | or 6.
When the main component of the component (B) is vitamin A, the supplement for improving intestinal environment and intestinal tract barrier comprising 100 IU to 60,000 IU as vitamin A contained in one recommended intake. In any one of Claims 1 thru | or 6.
When the main component of the component (B) is vitamin D, the supplement for improving intestinal environment and intestinal barrier, comprising 100 IU to 10,000 IU as vitamin D contained in one recommended intake. In claim 1,
The component (A) includes probiotics that are spore-forming lactic acid bacteria, and prebiotics that are oligosaccharides, cyclic oligosaccharides, and inulin. The component (B) includes zinc gluconate, vitamin A, and An intestinal environment and intestinal barrier improving supplement characterized by comprising In claim 11,
The component (C) contains calcium as shellfish calcium, magnesium compound as magnesium oxide, protein as soybean peptide, and amino acid as L methionine, and an intestinal environment and intestinal barrier improving supplement, .