JP2014033646A - Additive for rice flour bread, rice flour composition for rice flour bread, bread dough for rice flour bread, and production method of rice flour bread - Google Patents

Additive for rice flour bread, rice flour composition for rice flour bread, bread dough for rice flour bread, and production method of rice flour bread Download PDF

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JP2014033646A
JP2014033646A JP2012176690A JP2012176690A JP2014033646A JP 2014033646 A JP2014033646 A JP 2014033646A JP 2012176690 A JP2012176690 A JP 2012176690A JP 2012176690 A JP2012176690 A JP 2012176690A JP 2014033646 A JP2014033646 A JP 2014033646A
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rice flour
bread
flour bread
dough
salt
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JP6083730B2 (en
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Yasuko Konishi
康子 小西
Yuji Honda
裕司 本多
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Ishikawa Prefectural Public University Corp
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Abstract

PROBLEM TO BE SOLVED: To provide a food additive for improving the quality of a gluten-free rice flour bread, i.e. achieving fluffiness (high expansivity), softness, slow aging, and nicely-browned color; and a low-salt rice flour bread in response to the recent health conscious trends.SOLUTION: Addition of protease derived from Bacillus stearothermophilus to a dough derived from gluten-free rice flour allows a bread to be produced to achieve nicely-browned color, fluffiness, softness, and slow aging. Furthermore, the bread can be produced without addition of salt.

Description

本発明は、米粉パン用添加剤、米粉パン用米粉組成物、米粉パン用パン生地及び米粉パンの製造方法、並びに減塩パンに関する。   The present invention relates to an additive for rice flour bread, a rice flour composition for rice flour bread, a bread dough for rice flour bread, a method for producing rice flour bread, and a reduced salt bread.

小麦に含まれるグルテンの摂取によるアレルギーやセリアック病などの疾患の存在からグルテンフリー米粉パンの開発が求められている。また、米の消費拡大、食料自給率向上への取り組みの一つとしても米粉パンは注目されている。
しかしながら、米粉はパンの骨格となるグルテンを含有していないため、イーストの発酵によって生じる炭酸ガスの保持が困難であることや、小麦パンと比較して焼成後のパンの老化が速い等の問題がある。
Development of gluten-free rice flour bread has been demanded due to the presence of allergies and celiac disease due to the intake of gluten contained in wheat. Rice flour bread is also attracting attention as one of the efforts to expand rice consumption and improve the food self-sufficiency rate.
However, because rice flour does not contain gluten, which is the skeleton of bread, it is difficult to hold carbon dioxide produced by yeast fermentation, and bread aging after baking is faster than wheat bread. There is.

米粉パンに関する報告は以下の通りである。   The report about rice flour bread is as follows.

特許文献1は、「米粉パン用米粉組成物は穀粉100重量部中、米粉75〜85重量部、グルテン14〜22重量部及びアルファ化小麦粉又はアルファ化コーンスターチ1〜5重量部を含有する」を開示している。
しかしながら、特許文献1に開示の米粉パンは、本発明に関する米粉パンとは異なり、グルテンが含まれている。
Patent Document 1 states that “a rice flour composition for rice flour bread contains 75 to 85 parts by weight of rice flour, 14 to 22 parts by weight of gluten and 1 to 5 parts by weight of pregelatinized flour or pregelatinized corn starch” in 100 parts by weight of flour. Disclosure.
However, unlike the rice flour bread according to the present invention, the rice flour bread disclosed in Patent Document 1 contains gluten.

特許文献2は、「グルテン含有米粉を用いる製パンにおいて、細胞壁分解酵素類、アミラーゼ類、食品用乳化剤及びイーストの発酵促進剤の1種又は2種以上を適量添加することを特徴とする米粉パンの製造法」を開示している。
しかしながら、特許文献2に開示の米粉パンは、本発明に関する米粉パンとは異なり、グルテンが含まれている。
Patent Document 2 states that “a rice flour bread characterized by adding an appropriate amount of one or more of cell wall degrading enzymes, amylases, food emulsifiers and yeast fermentation promoters in bread making using gluten-containing rice flour. Is disclosed.
However, the rice flour bread disclosed in Patent Document 2 contains gluten, unlike the rice flour bread according to the present invention.

特許文献3は、「(1)生米粒をアルカリ性水溶液とともに粉砕処理するか、または生米粒を粉砕した米粉をアルカリ性水溶液に浸漬処理し、処理後の米粉とアルカリ性水溶液とを分離する工程、(2)分離した米粉をアルカリ性水溶液に浸漬処理し、処理後の米粉とアルカリ性水溶液とを分離する工程、但し、この工程は、1回または2回以上行う、(3)得られた米粉を、任意に水洗及び/又は中和した後に、脱水及び/又は乾燥して米粉製品を得る工程を含む米粉製品の製造方法」を開示している。
しかしながら、段落「0046」は「米粉を25.5%含有する生地にもかかわらず、比容積が最も大きく総合的に最も良好な製パン性を示した。」を開示し、段落「0053」は「試験例6(アルカリ処理米粉C27%)は、米粉を27%含有する生地であるにもかかわらず、総合的に最も良好な製パン性を示した。」を開示している。
前記開示から明らかなように、特許文献3に開示の米粉パンは、本発明に関する米粉パンとは異なり、米粉100%を使用することができないと考えられる。
Patent Document 3 states that “(1) A process in which raw rice grains are pulverized with an alkaline aqueous solution, or rice flour obtained by pulverizing raw rice grains is immersed in an alkaline aqueous solution to separate the treated rice flour from the alkaline aqueous solution, (2 ) A step of immersing the separated rice flour in an alkaline aqueous solution and separating the treated rice flour from the alkaline aqueous solution, provided that this step is performed once or twice or more. (3) The obtained rice flour is optionally "A method for producing a rice flour product including a step of obtaining a rice flour product by dehydration and / or drying after washing and / or neutralization" is disclosed.
However, Paragraph “0046” discloses “Despite the dough containing 25.5% rice flour, the specific volume was the largest and the overall best bread-making property was exhibited.” Paragraph “0053” "Test Example 6 (alkali-treated rice flour C27%) showed the best overall bread-making properties despite being a dough containing 27% rice flour."
As apparent from the above disclosure, it is considered that the rice flour bread disclosed in Patent Document 3 cannot use 100% rice flour, unlike the rice flour bread according to the present invention.

特許文献4は、「米粉を用いたパンの製造において、酵母による発酵前に、Aspergillus属に属する微生物、及び/又はRhizopus属に属する微生物により前発酵をすることを特徴とする、米粉を用いたパン製造方法。」を開示している。
しかしながら、特許文献4に開示の米粉パンは、本発明に関する米粉パンとは異なり、麹等のカビによる前発酵工程を含んでいる。
Patent Document 4 states that “in the production of bread using rice flour, before fermentation with yeast, prefermentation is performed using a microorganism belonging to the genus Aspergillus and / or a microorganism belonging to the genus Rhizopus. Bread manufacturing method ".
However, the rice flour bread disclosed in Patent Document 4 includes a pre-fermentation step using mold such as rice cake, unlike the rice flour bread according to the present invention.

加えて、上記特許文献1〜4は、本発明の特徴である「Bacillus stearothermophilus由来のプロテアーゼを米粉由来の生地に添加すること」を開示又は示唆をしていない。 In addition, Patent Documents 1 to 4 do not disclose or suggest “adding a Bacillus stearothermophilus- derived protease to rice flour-derived dough”, which is a feature of the present invention.

特開2011-113JP2011-113 特開2004-208561JP2004-208561 特開2012-95535JP2012-95535 特開2012-115197JP2012-115197

本発明は、米粉パン、特にグリテンフリーな米粉パンの品質向上{ふんわりとしている(膨張率が高い)、柔らかい、老化が遅い、焼色が良い}を目的とし、品質に改善効果を与える可能性のある様々な食品成分の添加効果について検討した。さらに、近年の健康志向の上昇に伴う減塩米粉パンの製造についても検討した。   The present invention aims to improve the quality of rice flour bread, particularly gluten-free rice flour bread {soft (high expansion rate), soft, slow aging, good baking color} The effects of adding various food ingredients were investigated. Furthermore, the production of reduced-salt rice flour bread with the recent increase in health orientation was also examined.

本発明者らは、上記課題を解決するために、Bacillus stearothermophilus由来のプロテアーゼをグルテンフリーである米粉由来の生地に添加することにより、製造したパンの焼き色が良く、ふんわりとしており、柔らかく、さらに老化が遅いことを確認した。さらに、食塩を添加することなく、該パンを製造することもできることを確認した。これにより、本発明を完成した。 In order to solve the above problems, the present inventors have added a protease derived from Bacillus stearothermophilus to a dough derived from rice flour that is gluten-free so that the baked color of the produced bread is good, soft, soft, It was confirmed that aging was slow. Furthermore, it was confirmed that the bread could be produced without adding salt. This completed the present invention.

本発明は以下の通りである。
「1.Bacillus stearothermophilus由来のプロテアーゼを含有する米粉パン用添加剤。
2.Bacillus stearothermophilus由来のプロテアーゼ及び米粉を含有する米粉パン用米粉組成物。
3.Bacillus stearothermophilus由来のプロテアーゼ、米粉、酵母及び水分を含有する米粉パン用パン生地。
4.以下の工程を含む米粉パンの製造方法、
(1)少なくともBacillus stearothermophilus由来のプロテアーゼ、米粉、酵母及び水分を混合してパン生地を製造するパン生地製造工程、
(2)前記生地を成形して成形生地とする成形生地製造工程、
(3)前記成形生地を発酵させるホイロ工程、
(4)前記ホイロ工程後の成形生地を焼成する焼成工程。
5.前記(1)〜(4)のいずれの工程において、グルテン又はグルテンを含む成分を添加しないことを特徴とする前項4に記載の米粉パンの製造方法。
6.前記(1)〜(4)のいずれの工程において、食塩を添加しないことを特徴とする前項4又は5に記載の米粉パンの製造方法。
7.前項5の製造方法で得られたグルテンフリーの米粉パン。
8.前項6の製造方法で得られた減塩米粉パン。
9.前記減塩米粉パンの塩分は、米粉の含有量に対して1.0重量%以下である前項8に記載の減塩米粉パン。」
The present invention is as follows.
“1. An additive for rice flour bread containing protease derived from Bacillus stearothermophilus .
2. A rice flour composition for rice flour bread comprising a protease derived from Bacillus stearothermophilus and rice flour.
3. Bread dough for rice flour bread containing protease, rice flour, yeast and moisture derived from Bacillus stearothermophilus .
4). A method for producing rice flour bread comprising the following steps:
(1) A bread dough production process for producing bread dough by mixing at least a protease derived from Bacillus stearothermophilus , rice flour, yeast and moisture,
(2) A molded dough manufacturing process for forming the dough to form a dough;
(3) A proofing process for fermenting the molded dough,
(4) A firing step of firing the formed dough after the proofing step.
5. The method for producing rice flour bread according to 4 above, wherein gluten or a component containing gluten is not added in any of the steps (1) to (4).
6). 6. The method for producing rice flour bread according to 4 or 5 above, wherein salt is not added in any of the steps (1) to (4).
7). A gluten-free rice flour bread obtained by the production method of the preceding item 5.
8). Reduced salt rice flour bread obtained by the production method of the preceding item 6.
9. 9. The reduced-salt rice flour bread according to item 8, wherein the salt content of the reduced-salt rice flour bread is 1.0% by weight or less with respect to the rice flour content. "

本発明で得られる米粉由来の米粉パンが、従来の米粉パンと比較して、焼き色が良く、ふんわりとしており、柔らかく、さらに老化が遅い。加えて、食塩を添加することなく、該パンを製造できる。
加えて、本発明で得られる米粉由来の米粉パンが、市販の小麦パンより柔らかくすることができた。
The rice flour bread derived from the rice flour obtained in the present invention has a better baked color, is softer and softer than conventional rice flour breads, and is slow to age. In addition, the bread can be made without adding salt.
In addition, the rice flour bread derived from rice flour obtained in the present invention could be made softer than commercially available wheat bread.

米粉パンの製造工程及び基本材料Rice flour bread manufacturing process and basic materials 各プロテアーゼ添加後のインキュベートをしていない米粉パンの特性の確認(図中のカラーは、各パンの色を比較するために置いてある)Confirmation of the characteristics of rice flour bread that has not been incubated after addition of each protease (colors in the figure are provided to compare the color of each bread) 各プロテアーゼ添加後のインキュベートをした米粉パンの特性の確認Confirmation of characteristics of rice flour bread incubated after each protease addition サモアーゼ(登録商標)添加後のインキュベートの有無による米粉パンの特性の確認Confirmation of the characteristics of rice flour bread with and without incubation after addition of Samoaise (registered trademark) 様々な濃度のサモアーゼ(登録商標)添加による米粉パンの膨張率及び硬さの確認Confirmation of expansion rate and hardness of rice flour bread by adding various concentrations of Samoaze (registered trademark) 米粉パンの老化速度の確認(図中の「control」はサモアーゼ(登録商標)未添加及び未解凍の米粉パン、「解凍control」はサモアーゼ(登録商標)未添加及び解凍後の米粉パン、「サモアーゼ」はサモアーゼ(登録商標)添加及び未解凍の米粉パン、及び「解凍サモアーゼ」はサモアーゼ(登録商標)添加及び解凍後の米粉パン、を意味する)Confirmation of the rate of aging of the rice flour bread ( "control" in the figure Thermoase (registered trademark) was not added and the rice flour bread not been decompressed, "decompression control" is Thermoase (registered trademark) was not added and the rice flour bread after thawing, "thermoase "" Samoese (registered trademark) added and unthawed rice flour bread, and "thawing Samoaase" means Samoaase (registered trademark) added and thawed rice flour bread) 市販の小麦パンとの柔らかさの比較の確認(図中において、サモアーゼ(登録商標)未添加により製造した米粉パンの柔らかさを100%に設定した)Confirmation of comparison of softness with commercially available wheat bread (In the figure, the softness of rice flour bread produced by adding no Samoase (registered trademark) was set to 100%) 様々な濃度の塩分添加による米粉パンの膨張率の確認Confirmation of expansion rate of rice flour bread by adding various concentrations of salt

以下に、本発明を詳細に説明する。   The present invention is described in detail below.

(本発明の米粉パン用添加剤)
本発明の米粉パン用添加剤は、少なくともBacillus stearothermophilus由来のプロテアーゼを含有する。
Bacillus stearothermophilus由来のプロテアーゼの好ましい例として、サモアーゼ(登録商標)PC10F(天野エンザイム株式会社製品)が挙げられる。
また、Bacillus stearothermophilus由来のプロテアーゼは、下記実施例2の結果より、米粉300g当たり、5万〜100万ユニット、好ましくは10万〜80万ユニット、より好ましくは15万〜50万ユニット、最も好ましくは18万〜25万ユニットである。
加えて、本発明の米粉パン用添加剤は、好ましくは、基本材料(例、米粉、砂糖、塩、オリーブオイル、ドライイースト、水)に添加して、15〜30℃で5時間〜30時間インキュベートする。
(Additive for rice flour bread of the present invention)
The rice flour bread additive of the present invention contains at least a protease derived from Bacillus stearothermophilus .
A preferred example of a protease derived from Bacillus stearothermophilus is Samoaase (registered trademark) PC10F (Amano Enzyme Co., Ltd. product).
From the results of Example 2 below, the protease derived from Bacillus stearothermophilus is 50,000 to 1 million units, preferably 100,000 to 800,000 units, more preferably 150,000 to 500,000 units, most preferably, per 300 g of rice flour. 180,000-250,000 units.
In addition, the rice flour bread additive of the present invention is preferably added to a basic material (eg, rice flour, sugar, salt, olive oil, dry yeast, water) at 15-30 ° C. for 5-30 hours. Incubate.

(本発明の米粉パン用米粉組成物)
本発明の米粉パン用米粉組成物は、少なくともBacillus stearothermophilus由来のプロテアーゼ及び米粉を含有する。
(Rice flour composition for rice flour bread of the present invention)
The rice flour composition for rice flour bread of the present invention contains at least a protease derived from Bacillus stearothermophilus and rice flour.

(本発明の米粉パン用パン生地)
本発明の米粉パン用パン生地は、少なくとも、Bacillus stearothermophilus由来のプロテアーゼ、米粉、酵母及び水分を含む。各成分量は、米粉100重量部に対して、糖類1〜15重量部、塩1〜10重量部、オイル2〜20重量部、酵母(ドライイースト)2〜10重量部、水分50〜350重量部である。より好ましい成分量は、米粉300重量部に対して、糖類6〜12重量部、塩2〜8重量部、オイル5〜15重量部、酵母(ドライイースト)2.1〜6.3重量部、水分200〜260重量部である。
なお、以下の実施例4の結果より、本発明の米粉パン用パン生地は食塩を含有しなくても、優れた品質の米粉パンを製造することができる。
さらに、本発明の米粉パン用パン生地は、冷凍が可能であり、解凍後はそのまま使用することができる。さらに、解凍後の米粉パン用パン生地は、未冷凍の米粉パン用パン生地と同様な品質の米粉パンを製造することができる。
加えて、自体公知の風味成分を本発明の米粉パン用パン生地に添加しても良い。
(Bread dough for rice flour bread of the present invention)
The bread dough for rice flour bread of the present invention contains at least a protease derived from Bacillus stearothermophilus , rice flour, yeast and moisture. The amount of each component is 1 to 15 parts by weight of sugar, 1 to 10 parts by weight of salt, 2 to 20 parts by weight of oil, 2 to 10 parts by weight of yeast (dry yeast), and 50 to 350 parts by weight of water with respect to 100 parts by weight of rice flour. Part. More preferable component amounts are 6 to 12 parts by weight of sugar, 2 to 8 parts by weight of salt, 5 to 15 parts by weight of oil, 2.1 to 6.3 parts by weight of yeast (dry yeast), and 200 to 260 parts of water with respect to 300 parts by weight of rice flour. Parts by weight.
In addition, from the result of the following Example 4, even if the bread dough for rice flour bread | pan of this invention does not contain salt, it can manufacture the rice flour bread | pan of outstanding quality.
Furthermore, the bread dough for rice flour bread of the present invention can be frozen and used as it is after thawing. Furthermore, the baked dough for rice flour bread can produce a rice flour bread having the same quality as that of unfrozen rice flour bread dough.
In addition, flavor components known per se may be added to the bread dough for rice flour breads of the present invention.

(各成分)
本発明の米粉、酵母、水分、オイル及び糖類は自体公知(特に、市販品)を利用することができる。例えば、以下の通りである。
米粉は、生米を粉末化して製造する。米の種類としては、特に限定されないが、精白米、玄米、屑米、古米等を挙げられるが、精白米であることが好ましい。
酵母は、天然酵母、ドライイースト、生イーストのいずれも使用することができる。
水分は、純粋な水に限定されず、牛乳等も使用することができる。
オイルは、食用オイルであれば特に限定されず、オリーブオイル、サラダオイル等を挙げられるが、オリーブオイルが好ましい。
糖類は、特に限定されないが、砂糖、マルトース、トレハロース、フルクトース、マルチトール、ソルビト−ル、キシリトール、及びそれらの併用が挙げられるが、砂糖であることが好ましい。
(Each component)
The rice flour, yeast, moisture, oil and saccharides of the present invention can be known per se (in particular, commercially available products). For example, it is as follows.
Rice flour is produced by pulverizing raw rice. Although it does not specifically limit as a kind of rice, Although polished rice, brown rice, waste rice, an old rice, etc. are mentioned, It is preferable that it is polished rice.
As the yeast, any of natural yeast, dry yeast, and fresh yeast can be used.
Water is not limited to pure water, and milk or the like can also be used.
The oil is not particularly limited as long as it is an edible oil, and examples thereof include olive oil and salad oil. Olive oil is preferred.
The saccharide is not particularly limited, and examples thereof include sugar, maltose, trehalose, fructose, maltitol, sorbitol, xylitol, and combinations thereof, but sugar is preferable.

(米粉パンの製造方法)
本発明の米粉パンは、少なくとも以下の工程を含む。なお、製造するパンの種類によって、下記工程中に、パン生地を休ませるためのベンチ工程、生地を冷やすための生地冷却工程、パンの形状を作成するための分割工程を含んでも良い。加えて、米粉パンを製造可能な市販のホームベーカリーを使用する場合には、添付のマニュアルに従って製造すれば良い。
(1)少なくともBacillus stearothermophilus由来のプロテアーゼ、米粉、酵母及び水分を混合してパン生地を製造するパン生地製造工程、
(2)前記生地を成形して成形生地とする成形生地製造工程、
(3)前記成形生地を発酵させるホイロ工程、
(4)前記ホイロ工程後の成形生地を焼成する焼成工程。
なお、好ましくは、上記(1)の工程中において、Bacillus stearothermophilus由来のプロテアーゼを添加した後に、15〜30℃で5時間〜30時間インキュベートする。
さらに、好ましくは、前記(1)〜(4)のいずれの工程において、グルテン又はグルテンを含む成分を添加しないことによりグルテンフリーな米粉パンを製造することができる。
さらに、好ましくは、前記(1)〜(4)のいずれの工程において、食塩を添加しないことにより減塩米粉パンを製造することができる。
加えて、自体公知の風味成分を前記(1)〜(4)のいずれかの工程で添加しても良い。
(Manufacturing method of rice flour bread)
The rice flour bread of the present invention includes at least the following steps. Depending on the type of bread to be manufactured, the following processes may include a bench process for resting the dough, a dough cooling process for cooling the dough, and a dividing process for creating the shape of the bread. In addition, when using a commercially available home bakery capable of producing rice flour bread, it may be manufactured according to the attached manual.
(1) A bread dough production process for producing bread dough by mixing at least a protease derived from Bacillus stearothermophilus , rice flour, yeast and moisture,
(2) A molded dough manufacturing process for forming the dough to form a dough;
(3) A proofing process for fermenting the molded dough,
(4) A firing step of firing the formed dough after the proofing step.
In addition, Preferably, in the process of said (1), after adding the protease derived from Bacillus stearothermophilus , it incubates at 15-30 degreeC for 5 to 30 hours.
Furthermore, Preferably, in any process of said (1)-(4), a gluten-free rice flour bread | pan can be manufactured by not adding the component containing a gluten or gluten.
Furthermore, Preferably, in any process of said (1)-(4), a salt-reduced rice flour bread | pan can be manufactured by not adding salt.
In addition, a flavor component known per se may be added in any of the steps (1) to (4).

(米粉パン)
本発明の米粉パンは、特に限定されずに、食パン、フランスパン、クロッワッサン、ロールパン、ベーグル、イングリッシュマフィン、菓子パン等が挙げられる。
(Rice flour bread)
The rice flour bread of the present invention is not particularly limited, and examples thereof include bread, French bread, croissant, roll bread, bagel, English muffin, and sweet bread.

(本発明の米粉パン)
本発明の米粉パンは、下記実施例1〜4から明らかなように、従来の米粉パンと比較して、焼き色が良く、ふんわりとしており、柔らかく、さらに老化が遅い。
さらに、食塩を添加することなく、該パンを製造できる。すなわち、本発明の米粉パンは、健康志向である減塩米粉パンも含む。なお、減塩米粉パンの塩分濃度は、米粉の含有量に対して1.0重量%以下、好ましくは0.5重量%以下、より好ましくは0.4重量%以下、最も好ましくは0.3重量%以下である。
加えて、本発明で得られるグルテンフリーである米粉由来の米粉パンが、市販の小麦パンより柔らかいことも確認した。
(Rice flour bread of the present invention)
As is clear from Examples 1 to 4 below, the rice flour bread of the present invention has a better baked color, is softer and softer, and is slower to age than conventional rice flour breads.
Furthermore, the bread can be produced without adding salt. That is, the rice flour bread of the present invention also includes reduced-salt rice flour bread that is health-oriented. The salt concentration of the reduced-salt rice flour bread is 1.0% by weight or less, preferably 0.5% by weight or less, more preferably 0.4% by weight or less, and most preferably 0.3% by weight or less based on the rice flour content.
In addition, it was also confirmed that rice flour bread derived from rice flour that is gluten-free obtained in the present invention is softer than commercially available wheat bread.

以下、実施例を挙げて本発明を説明するが、本発明はこれら実施例により何ら限定されるものではない。   EXAMPLES Hereinafter, although an Example is given and this invention is demonstrated, this invention is not limited at all by these Examples.

(各プロテアーゼ添加による米粉パンの特性の確認)
本実施例では、各プロテアーゼを生地に添加することによって製造した米粉パンの特性を確認した。詳細は、以下の通りである。
(Confirmation of the characteristics of rice flour bread by adding each protease)
In this example, the characteristics of rice flour bread produced by adding each protease to the dough were confirmed. Details are as follows.

(米粉パンの製造方法)
米粉パンの製造には、米粉パンを製造可能な市販のホームベーカリー(Panasonic SD-BH103)を使用した。製造工程は、以下の通りである(参照:図1)。
(1)基本材料である300gの米粉(福盛シトギ2号)、9gの砂糖、5gの塩、10gのオリーブオイル、4.2gのドライイースト及び230gの水を前記ベーカリー内に添加した。
(2−1)1.9×105 UのBacillus stearothermophilus由来のプロテアーゼ{サモアーゼ(登録商標)PC10F(天野エンザイム株式会社製品)}をさらにベーカリー内に添加した。
(2−2)コントロールとして、市販のプロテアーゼA(1.9×105 U、天野エンザイム株式会社製品)をさらにベーカリー内に添加した。
(2−3)コントロールとして、市販のプロテアーゼM(1.9×105 U、天野エンザイム株式会社製品)をさらにベーカリー内に添加した。
(2−4)コントロールとして、市販のプロテアーゼP(1.9×105 U、天野エンザイム株式会社製品)をさらにベーカリー内に添加した。
(2−5)コントロールとして、プロテアーゼを添加していないものを用意した。
(3−1)上記(2−1)、(2−2)、(2−3)及び(2−4)において、25℃、16時間でインキュベートした。
(3−2)上記(2−1)、(2−2)、(2−3)、(2−4)及び(2−5)において、インキュベートしていないものを用意した。
(4)前記ベーカリーの米粉パン(小麦なし)モードで焼成まで行った。
(5)焼成終了後、室温、2時間で放冷して、米粉パンを得た。
(6)前記米粉パンの容積を菜種置換法により測定した。さらに、膨張率(容積/重量×100
)も測定した。
(7)最後に、前記米粉パンの硬さをレオメーター(CR-500DX、株式会社サン科学社製品を用いて測定した。
(Manufacturing method of rice flour bread)
For the production of rice flour bread, a commercially available home bakery (Panasonic SD-BH103) capable of producing rice flour bread was used. The manufacturing process is as follows (see FIG. 1).
(1) 300 g rice flour (Fukumori Shitogi No. 2), which is a basic material, 9 g sugar, 5 g salt, 10 g olive oil, 4.2 g dry yeast and 230 g water were added into the bakery.
(2-1) 1.9 × 10 5 U Bacillus stearothermophilus- derived protease {Samoase (registered trademark) PC10F (Amano Enzyme Co., Ltd. product)} was further added into the bakery.
(2-2) As a control, commercially available protease A (1.9 × 10 5 U, Amano Enzyme Co., Ltd.) was further added to the bakery.
(2-3) Commercially available protease M (1.9 × 10 5 U, Amano Enzyme Co., Ltd.) was further added to the bakery as a control.
(2-4) As a control, commercially available protease P (1.9 × 10 5 U, Amano Enzyme Co., Ltd.) was further added to the bakery.
(2-5) As a control, a sample to which no protease was added was prepared.
(3-1) In the above (2-1), (2-2), (2-3) and (2-4), the cells were incubated at 25 ° C. for 16 hours.
(3-2) In (2-1), (2-2), (2-3), (2-4) and (2-5) above, an unincubated one was prepared.
(4) Baking was performed in the bakery rice flour bread (without wheat) mode.
(5) After baking, the mixture was allowed to cool at room temperature for 2 hours to obtain rice flour bread.
(6) The volume of the rice flour bread was measured by a rapeseed replacement method. Furthermore, expansion rate (volume / weight x 100
) Was also measured.
(7) Finally, the hardness of the rice flour bread was measured using a rheometer (CR-500DX, Sun Science Co., Ltd. product).

(プロテアーゼ添加後のインキュベートをしていない米粉パンの特性評価の結果)
プロテアーゼ添加後のインキュベートをしていない米粉パンの特性を図2に示す。なお、コントロールはプロテアーゼを添加しないで製造した米粉パンである。
図2から明らかなように、サモアーゼ(登録商標)を添加して製造した米粉パンは、膨らみ、さらにクラムの気泡が小さかった。一方、プロテアーゼA、M、Pを添加して製造した米粉パンは、空洞が生じた。
以上により、サモアーゼ(登録商標)を添加することにより、米粉パンを膨らませることができ、さらにクラムの気泡を小さくできることを確認した。
(Results of characteristic evaluation of rice flour bread not incubated after addition of protease)
FIG. 2 shows the characteristics of rice flour bread that has not been incubated after addition of protease. The control is rice flour bread manufactured without adding protease.
As is clear from FIG. 2, the rice flour bread produced by adding Samoaze (registered trademark) swelled, and the crumb bubbles were small. On the other hand, the rice flour bread produced by adding protease A, M, and P had voids.
From the above, it was confirmed that by adding Samoaze (registered trademark) , the rice flour bread could be expanded and the crumb bubbles could be further reduced.

(プロテアーゼ添加後のインキュベートをした米粉パンの特性評価の結果)
プロテアーゼ添加後のインキュベートをした米粉パンの特性を図3に示す。なお、コントロールはプロテアーゼを添加しないで製造した米粉パンである。
上記段落「0026」の結果と同様に、サモアーゼ(登録商標)を添加して製造した米粉パンは、膨らみ、さらにクラムの気泡が小さかった。一方、プロテアーゼA、Pを添加して製造した米粉パンは、空洞が生じた。
さらに、サモアーゼ(登録商標)を添加して製造した米粉パンの外皮の色は、きれいな焼き色であった。一方、プロテアーゼMを添加して製造した米粉パンの外皮の色は、かなり黒くなり、焦げたようになった。
以上により、サモアーゼ(登録商標)を添加することにより、米粉パンの外皮の色をきれいな焼き色にできることを確認した。
(Result of characteristic evaluation of rice flour bread incubated after addition of protease)
FIG. 3 shows the characteristics of the rice flour bread incubated after the addition of protease. The control is rice flour bread manufactured without adding protease.
Similar to the result of the above paragraph “0026”, the rice flour bread produced by adding Samoaze (registered trademark) swelled and the crumb bubbles were small. On the other hand, the rice flour bread produced by adding protease A and P had cavities.
Furthermore, the color of the hull of the rice flour bread produced by adding Samoaise (registered trademark) was a beautiful baked color. On the other hand, the color of the hull of the rice flour bread manufactured by adding protease M became considerably black and burnt.
From the above, it was confirmed that by adding Samoaze (registered trademark) , the skin color of the rice flour bread could be made a beautiful baked color.

(プロテアーゼ添加後のインキュベートの効果の確認)
プロテアーゼ添加後のインキュベートの有無による米粉パンの特性を図4に示す。
プロテアーゼ添加後のインキュベートをした米粉パンは、プロテアーゼ添加後のインキュベートをしていない米粉パンと比較して、硬さ(0.6N)は同じであるが、膨張率が向上した。
以上により、サモアーゼ(登録商標)を添加した後のインキュベートにより、米粉パンをふんわりさせる(膨張率を上げる)ことができることを確認した。
(Confirmation of the effect of incubation after addition of protease)
FIG. 4 shows the characteristics of rice flour bread with and without incubation after addition of protease.
The rice flour bread that had been incubated after the addition of protease had the same hardness (0.6 N) as compared to the rice flour bread that had not been incubated after the addition of protease, but the expansion rate was improved.
From the above, it was confirmed that the rice flour bread can be softened ( increased expansion rate) by incubation after adding Samoaze (registered trademark) .

(最適なサモアーゼ(登録商標)添加量の確認)
上記実施例1より最適な条件として、サモアーゼ(登録商標)添加及び該添加後のインキュベートを行う条件を選択した。そして、該条件下での最適なサモアーゼ(登録商標)添加量を確認した。詳細は、以下の通りである。
(Confirmation of the optimal amount of Samoaese (registered trademark) )
As conditions more optimal than those in Example 1, conditions for adding Samoaase (registered trademark) and incubating after the addition were selected. Then, the optimum amount of Samoaase (registered trademark) added under the conditions was confirmed. Details are as follows.

(様々な濃度のサモアーゼ(登録商標)添加による米粉パンの製造)
サモアーゼ(登録商標)添加及び該添加後のインキュベートを行う条件を選択して上記実施例1と同様に、様々な濃度のサモアーゼ(登録商標)添加による米粉パンを製造した。
(Manufacturing rice flour bread with various concentrations of Samoase (registered trademark) )
Rice flour breads with various concentrations of Samoaase (registered trademark) were prepared in the same manner as in Example 1 above by selecting the conditions for adding Samoaase (registered trademark) and incubation after the addition.

様々な濃度のサモアーゼ(登録商標)添加による米粉パンの膨張率及び硬さを図5に示した。
図5から明らかなように、添加量に順じて膨張率が増加し、硬さが減少した。さらに、10万ユニットのサモアーゼ(登録商標)添加により十分な膨張率及び柔らかさを得ることができた。
以上により、サモアーゼ(登録商標)添加量は、米粉300g当たり、5万〜100万ユニット、好ましくは10万〜80万ユニット、より好ましくは15万〜50万ユニット、最も好ましくは18万〜25万ユニットであることを確認した。
FIG. 5 shows the expansion rate and hardness of rice flour breads with various concentrations of Samoaze (registered trademark) added.
As is clear from FIG. 5, the expansion rate increased and the hardness decreased according to the amount added. Furthermore, sufficient expansion rate and softness could be obtained by adding 100,000 units of Samoaise (registered trademark) .
As described above, the amount of Samoaase (registered trademark) added is 50,000 to 1 million units, preferably 100,000 to 800,000 units, more preferably 150,000 to 500,000 units, most preferably 180,000 to 250,000 per 300 g of rice flour. Confirmed unit.

(老化速度及び市販の小麦パンとの品質比較の確認)
上記実施例2より最適なサモアーゼ(登録商標)添加量として、酵素添加量とパンの品質改善効果の関係から20万ユニットのサモアーゼ(登録商標)添加量の条件を選択した。そして、該条件下で製造した米粉パンの老化速度の確認及び市販の小麦パンとの品質比較を確認した。詳細は、以下の通りである。
(Confirmation of aging rate and quality comparison with commercial wheat bread)
As the optimal amount of Samoaase (registered trademark) added from Example 2, the condition of 200,000 units of Samoaase (registered trademark) added was selected from the relationship between the amount of enzyme added and the effect of improving the quality of bread. And the confirmation of the aging rate of the rice flour bread manufactured on this condition and the quality comparison with the commercially available wheat bread were confirmed. Details are as follows.

(20万ユニットのサモアーゼ(登録商標)添加による米粉パンの製造)
20万ユニットのサモアーゼ(登録商標)添加及び該添加後のインキュベートを行う条件を選択して上記実施例1と同様に、様々な濃度のサモアーゼ(登録商標)添加による米粉パンを製造した。
(Manufacturing rice flour bread by adding 200,000 units of Samoaze (registered trademark) )
Rice flour breads with various concentrations of Samoaase (registered trademark) added were prepared in the same manner as in Example 1 above by selecting conditions for adding 200,000 units of Samoaase (registered trademark) and incubation after the addition.

(米粉パンの老化速度の測定)
従来の米粉パンは老化速度が速いという特徴があるため、米粉パンの老化速度について測定した。また、米粉パンを一度冷凍し、その後解凍した米粉パンの老化速度についても測定した。
上記段落「0033」で製造した米粉パンにおいて、冷凍していない米粉パン、一度冷凍した後解凍した米粉パン(0日、1日、2日目)を25℃の飽和水蒸気中で30分間インキュベートした後に、硬さを測定した。
なお、コントロールとして、サモアーゼ(登録商標)未添加によって製造した米粉パンでも同様な実験を行った。
(Measurement of aging rate of rice flour bread)
Since conventional rice flour bread is characterized by a fast aging rate, the aging rate of rice flour bread was measured. Moreover, the aging rate of the rice flour bread which was frozen once and then thawed was also measured.
In the rice flour bread produced in the above paragraph “0033”, the rice flour bread that has not been frozen and the rice flour bread that has been frozen once and thawed (day 0, day 1, day 2) were incubated in saturated steam at 25 ° C. for 30 minutes. Later, the hardness was measured.
As a control, the same experiment was performed on rice flour bread produced without addition of Samoaze (registered trademark) .

(米粉パンの老化速度の測定結果)
米粉パンの老化速度を示す実験結果を図6に示す。
図6の結果から明らかなように、冷凍の有無にかかわらずサモアーゼ(登録商標)を添加して製造した米粉パンは、サモアーゼ(登録商標)未添加と比較して、時間を経過しても柔らかさを維持していた。
以上により、サモアーゼ(登録商標)を添加することにより、米粉パンの老化速度を抑えることができることを確認した。
(Measurement result of aging rate of rice flour bread)
The experimental result which shows the aging speed | rate of rice flour bread | pan is shown in FIG.
As can be seen from the results in FIG. 6, rice bread manufactured by adding the Thermoase (registered trademark) or without freezing, thermoase compared to (R) was not added, soft even after time I was maintaining it.
From the above, it was confirmed that the aging rate of rice flour bread could be suppressed by adding Samoaze (registered trademark) .

(市販の小麦パンとの特性評価の結果)
上記段落「0033」で製造した米粉パンの柔らかさを、市販の小麦パンの柔らかさと比較した結果を図7に示す。
図7の結果から明らかなように、サモアーゼ(登録商標)を添加して製造した米粉パンは、市販の小麦パンと比較して、柔らかかった。
以上により、本発明で得られる米粉由来の米粉パンが、市販の小麦パンより柔らかくすることができることを確認した。
(Results of characteristic evaluation with commercially available wheat bread)
FIG. 7 shows the result of comparing the softness of the rice flour bread produced in the above paragraph “0033” with the softness of commercially available wheat bread.
As is clear from the results of FIG. 7, the rice flour bread produced by adding Samoaze (registered trademark) was softer than the commercially available wheat bread.
From the above, it was confirmed that the rice flour bread derived from rice flour obtained in the present invention can be made softer than commercially available wheat bread.

(減塩米粉パンの特性評価)
現在、健康志向により塩分を抑えた食品が多数開発されそして販売されている。
本実施例では、塩分未添加により製造した米粉パンが、ふんわりしている(膨張率が高い)かどうかを確認した。詳細は以下の通りである。
(Characteristic evaluation of low-salt rice flour bread)
Currently, many foods with reduced salt content have been developed and marketed for health reasons.
In this example, it was confirmed whether the rice flour bread produced by adding no salt was fluffy (high expansion rate). Details are as follows.

(減塩米粉パンの製造)
20万ユニットのサモアーゼ(登録商標)添加及び該添加後のインキュベートを行う条件を選択して、さらに食塩添加濃度(0g、2.5g、5.0g)の変更以外は、上記実施例1と同様に、米粉パンを製造した。
(Manufacture of reduced salt rice flour bread)
In the same manner as in Example 1 except that 200,000 units of Samoaze (registered trademark) were added and the conditions for incubation after the addition were selected, and the salt addition concentration (0 g, 2.5 g, 5.0 g) was changed. Rice flour bread was produced.

(減塩米粉パンの特性評価の結果)
様々な濃度の塩分添加による米粉パンの膨張率を図8に示した。
図8から明らかなように、サモアーゼ(登録商標)を添加して製造した米粉パンは、食塩添加量を減らしても、膨張率は低下しなかった。一方、サモアーゼ(登録商標)を添加せずに製造した米粉パン(コントロール)は、食塩添加量減少に伴い膨張率が減少した。
以上により、サモアーゼ(登録商標)を添加することにより、食塩を添加しなくても高い膨張率を維持できる米粉パンを製造できることを確認した。
(Results of characteristic evaluation of low-salt rice flour bread)
FIG. 8 shows the expansion rate of rice flour breads with various concentrations of added salt.
As apparent from FIG. 8, the expansion rate of the rice flour bread produced by adding Samoaze (registered trademark) did not decrease even when the amount of salt added was reduced. On the other hand, the expansion rate of rice flour bread (control) produced without adding Samoaze (registered trademark) decreased as the amount of salt added decreased.
From the above, it was confirmed that by adding Samoaze (registered trademark) , it was possible to produce a rice flour bread capable of maintaining a high expansion rate without adding salt.

(総論)
本発明の米粉パンは、従来の米粉パンと比較して、ふんわりしており(膨張率が高い)、柔らかく、外皮の色がきれいな焼き色であり、さらに老化速度が遅い。
本発明の米粉パンは、市販の小麦パンと比較しても、柔らかい。
本発明の米粉パンは、食塩を添加しなくてもふんわりしている。
(General)
The rice flour bread of the present invention is fluffy (high expansion rate), soft and has a beautiful husk color as compared with conventional rice flour bread, and has a slow aging rate.
The rice flour bread of the present invention is soft even compared to commercially available wheat bread.
The rice flour bread of the present invention is soft without adding salt.

本発明では、焼き色が良く、ふんわりとしており、柔らかく、さらに老化が遅い米粉由来の米粉パン、並びに食塩を添加しなくてもふんわりしている米粉パン、を提供することができる。
In the present invention, a rice flour bread derived from rice flour that has a good baked color, is soft, soft, and is slow to age, and a rice flour bread that is fluffy without adding salt can be provided.

Claims (9)

Bacillus stearothermophilus由来のプロテアーゼを含有する米粉パン用添加剤。
Additive for rice flour bread containing protease derived from Bacillus stearothermophilus .
Bacillus stearothermophilus由来のプロテアーゼ及び米粉を含有する米粉パン用米粉組成物。
A rice flour composition for rice flour bread comprising a protease derived from Bacillus stearothermophilus and rice flour.
Bacillus stearothermophilus由来のプロテアーゼ、米粉、酵母及び水分を含有する米粉パン用パン生地。
Bread dough for rice flour bread containing protease, rice flour, yeast and moisture derived from Bacillus stearothermophilus .
以下の工程を含む米粉パンの製造方法、
(1)少なくともBacillus stearothermophilus由来のプロテアーゼ、米粉、酵母及び水分を混合してパン生地を製造するパン生地製造工程、
(2)前記生地を成形して成形生地とする成形生地製造工程、
(3)前記成形生地を発酵させるホイロ工程、
(4)前記ホイロ工程後の成形生地を焼成する焼成工程。
A method for producing rice flour bread comprising the following steps:
(1) A bread dough production process for producing bread dough by mixing at least a protease derived from Bacillus stearothermophilus , rice flour, yeast and moisture,
(2) A molded dough manufacturing process for forming the dough to form a dough;
(3) A proofing process for fermenting the molded dough,
(4) A firing step of firing the formed dough after the proofing step.
前記(1)〜(4)のいずれの工程において、グルテン又はグルテンを含む成分を添加しないことを特徴とする請求項4に記載の米粉パンの製造方法。
The method for producing rice flour bread according to claim 4, wherein gluten or a component containing gluten is not added in any of the steps (1) to (4).
前記(1)〜(4)のいずれの工程において、食塩を添加しないことを特徴とする請求項4又は5に記載の米粉パンの製造方法。
The method for producing rice flour bread according to claim 4 or 5, wherein no salt is added in any of the steps (1) to (4).
請求項5の製造方法で得られたグルテンフリーの米粉パン。
A gluten-free rice flour bread obtained by the production method according to claim 5.
請求項6の製造方法で得られた減塩米粉パン。
Reduced salt rice flour bread obtained by the production method of claim 6.
前記減塩米粉パンの塩分は、米粉の含有量に対して1.0重量%以下である請求項8に記載の減塩米粉パン。   The reduced-salt rice flour bread according to claim 8, wherein the salt content of the reduced-salt rice flour bread is 1.0% by weight or less based on the content of the rice flour.
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