JP2013541347A - 液滴中の断片化ゲノムｄｎａの分析 - Google Patents

液滴中の断片化ゲノムｄｎａの分析 Download PDF

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Publication number
JP2013541347A
JP2013541347A JP2013537769A JP2013537769A JP2013541347A JP 2013541347 A JP2013541347 A JP 2013541347A JP 2013537769 A JP2013537769 A JP 2013537769A JP 2013537769 A JP2013537769 A JP 2013537769A JP 2013541347 A JP2013541347 A JP 2013541347A
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Japan
Prior art keywords
droplets
droplet
dna
genomic dna
nanoliters
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Pending
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JP2013537769A
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Japanese (ja)
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JP2013541347A5 (de
Inventor
ケヴィン・ディー・ニス
エイミー・エル・ヒデッセン
ポール・ダブリュー・ワイアット
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バイオ−ラッド・ラボラトリーズ・インコーポレーテッド
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Priority claimed from US12/976,827 external-priority patent/US9598725B2/en
Application filed by バイオ−ラッド・ラボラトリーズ・インコーポレーテッド filed Critical バイオ−ラッド・ラボラトリーズ・インコーポレーテッド
Publication of JP2013541347A publication Critical patent/JP2013541347A/ja
Publication of JP2013541347A5 publication Critical patent/JP2013541347A5/ja
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
JP2013537769A 2010-11-01 2011-11-01 液滴中の断片化ゲノムｄｎａの分析 Pending JP2013541347A (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US40910610P 2010-11-01 2010-11-01
US61/409,106 2010-11-01
US12/976,827 US9598725B2 (en) 2010-03-02 2010-12-22 Emulsion chemistry for encapsulated droplets
US12/976,827 2010-12-22
PCT/US2011/058854 WO2012061442A1 (en) 2010-11-01 2011-11-01 Analysis of fragmented genomic dna in droplets

Publications (2)

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JP2013541347A true JP2013541347A (ja) 2013-11-14
JP2013541347A5 JP2013541347A5 (de) 2014-12-18

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JP2013537769A Pending JP2013541347A (ja) 2010-11-01 2011-11-01 液滴中の断片化ゲノムｄｎａの分析

Country Status (7)

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EP (1) EP2635708A4 (de)
JP (1) JP2013541347A (de)
CN (1) CN103443289A (de)
AU (1) AU2011323507A1 (de)
CA (1) CA2816702A1 (de)
SG (1) SG190076A1 (de)
WO (1) WO2012061442A1 (de)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10760116B2 (en) 2015-08-28 2020-09-01 Kabushiki Kaisha Dnaform Analysis kit, analyzer, and methods for analyzing template nucleic acid or target substance

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110740813B (zh) * 2016-11-28 2022-06-03 亚利桑那州立大学董事会 涉及连续流动液滴反应的系统和方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5555191A (en) * 1994-10-12 1996-09-10 Trustees Of Columbia University In The City Of New York Automated statistical tracker
US20050277125A1 (en) * 2003-10-27 2005-12-15 Massachusetts Institute Of Technology High-density reaction chambers and methods of use
US20100137163A1 (en) * 2006-01-11 2010-06-03 Link Darren R Microfluidic Devices and Methods of Use in The Formation and Control of Nanoreactors
US8383345B2 (en) * 2008-09-12 2013-02-26 University Of Washington Sequence tag directed subassembly of short sequencing reads into long sequencing reads
US9156010B2 (en) * 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
JPN6015045356; Ｂｅｎｊａｍｉｎ　Ｊ．　ＨＩＮＤＳＯＮ: Ａｎａｌｙｔｉｃａｌ　Ｃｈｅｍｉｓｔｒｙ Ｖｏｌ．８３, 20111028, Ｐａｇｅｓ　８６０４-８６１０ *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10760116B2 (en) 2015-08-28 2020-09-01 Kabushiki Kaisha Dnaform Analysis kit, analyzer, and methods for analyzing template nucleic acid or target substance

Also Published As

Publication number Publication date
AU2011323507A1 (en) 2013-06-13
EP2635708A1 (de) 2013-09-11
CA2816702A1 (en) 2012-05-10
SG190076A1 (en) 2013-06-28
CN103443289A (zh) 2013-12-11
WO2012061442A1 (en) 2012-05-10
EP2635708A4 (de) 2014-10-29

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