SG190076A1 - Analysis of fragmented genomic dna in droplets - Google Patents
Analysis of fragmented genomic dna in droplets Download PDFInfo
- Publication number
- SG190076A1 SG190076A1 SG2013033238A SG2013033238A SG190076A1 SG 190076 A1 SG190076 A1 SG 190076A1 SG 2013033238 A SG2013033238 A SG 2013033238A SG 2013033238 A SG2013033238 A SG 2013033238A SG 190076 A1 SG190076 A1 SG 190076A1
- Authority
- SG
- Singapore
- Prior art keywords
- droplets
- dna
- genomic dna
- per
- droplet
- Prior art date
Links
- 238000004458 analytical method Methods 0.000 title description 4
- 238000000034 method Methods 0.000 claims abstract description 70
- 238000003556 assay Methods 0.000 claims abstract description 24
- 108091008146 restriction endonucleases Proteins 0.000 claims description 11
- 238000000638 solvent extraction Methods 0.000 claims description 4
- 238000010008 shearing Methods 0.000 claims description 3
- 239000000523 sample Substances 0.000 description 52
- 241000282414 Homo sapiens Species 0.000 description 8
- 239000012071 phase Substances 0.000 description 7
- 238000013467 fragmentation Methods 0.000 description 6
- 238000006062 fragmentation reaction Methods 0.000 description 6
- 239000000839 emulsion Substances 0.000 description 5
- 238000003752 polymerase chain reaction Methods 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 238000013459 approach Methods 0.000 description 4
- 239000013068 control sample Substances 0.000 description 4
- 230000029087 digestion Effects 0.000 description 4
- 239000012634 fragment Substances 0.000 description 3
- 150000007523 nucleic acids Chemical class 0.000 description 3
- 102000039446 nucleic acids Human genes 0.000 description 3
- 108020004707 nucleic acids Proteins 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 239000008346 aqueous phase Substances 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 238000012203 high throughput assay Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000004094 surface-active agent Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000282693 Cercopithecidae Species 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- 241000700159 Rattus Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 241000251539 Vertebrata <Metazoa> Species 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 239000000090 biomarker Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 238000004581 coalescence Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 230000003467 diminishing effect Effects 0.000 description 1
- 238000007876 drug discovery Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000004945 emulsification Methods 0.000 description 1
- 238000001976 enzyme digestion Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 238000007834 ligase chain reaction Methods 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 230000003595 spectral effect Effects 0.000 description 1
- 238000004611 spectroscopical analysis Methods 0.000 description 1
- 238000005382 thermal cycling Methods 0.000 description 1
- 239000007762 w/o emulsion Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US40910610P | 2010-11-01 | 2010-11-01 | |
| US12/976,827 US9598725B2 (en) | 2010-03-02 | 2010-12-22 | Emulsion chemistry for encapsulated droplets |
| PCT/US2011/058854 WO2012061442A1 (en) | 2010-11-01 | 2011-11-01 | Analysis of fragmented genomic dna in droplets |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG190076A1 true SG190076A1 (en) | 2013-06-28 |
Family
ID=46024812
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG2013033238A SG190076A1 (en) | 2010-11-01 | 2011-11-01 | Analysis of fragmented genomic dna in droplets |
Country Status (7)
| Country | Link |
|---|---|
| EP (1) | EP2635708A4 (de) |
| JP (1) | JP2013541347A (de) |
| CN (1) | CN103443289A (de) |
| AU (1) | AU2011323507A1 (de) |
| CA (1) | CA2816702A1 (de) |
| SG (1) | SG190076A1 (de) |
| WO (1) | WO2012061442A1 (de) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3339447B1 (de) | 2015-08-28 | 2020-07-29 | Kabushiki Kaisha DNAFORM | Verfahren zur analyse einer template-nukleinsäure, verfahren zur analyse eines zielmaterials, kit zur analyse einer template-nukleinsäure oder eines zielmaterials und vorrichtung zur analyse einer template-nukleinsäure oder eines zielmaterials |
| EP3544737A1 (de) * | 2016-11-28 | 2019-10-02 | Arizona Board of Regents on behalf of Arizona State University | Systeme und verfahren im zusammenhang mit tropfenreaktion mit kontinuierlichem fluss |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5555191A (en) * | 1994-10-12 | 1996-09-10 | Trustees Of Columbia University In The City Of New York | Automated statistical tracker |
| WO2005043154A2 (en) * | 2003-10-27 | 2005-05-12 | Massachusetts Institute Of Technology | High density reaction chambers and methods of use |
| WO2007081386A2 (en) * | 2006-01-11 | 2007-07-19 | Raindance Technologies, Inc. | Microfluidic devices and methods of use |
| US8383345B2 (en) * | 2008-09-12 | 2013-02-26 | University Of Washington | Sequence tag directed subassembly of short sequencing reads into long sequencing reads |
| US9156010B2 (en) * | 2008-09-23 | 2015-10-13 | Bio-Rad Laboratories, Inc. | Droplet-based assay system |
-
2011
- 2011-11-01 EP EP11838712.5A patent/EP2635708A4/de not_active Withdrawn
- 2011-11-01 CN CN2011800528318A patent/CN103443289A/zh active Pending
- 2011-11-01 SG SG2013033238A patent/SG190076A1/en unknown
- 2011-11-01 JP JP2013537769A patent/JP2013541347A/ja active Pending
- 2011-11-01 WO PCT/US2011/058854 patent/WO2012061442A1/en active Application Filing
- 2011-11-01 AU AU2011323507A patent/AU2011323507A1/en not_active Abandoned
- 2011-11-01 CA CA2816702A patent/CA2816702A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| EP2635708A1 (de) | 2013-09-11 |
| JP2013541347A (ja) | 2013-11-14 |
| AU2011323507A1 (en) | 2013-06-13 |
| CA2816702A1 (en) | 2012-05-10 |
| WO2012061442A1 (en) | 2012-05-10 |
| CN103443289A (zh) | 2013-12-11 |
| EP2635708A4 (de) | 2014-10-29 |
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