JP2013158337A5 - - Google Patents

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JP2013158337A5
JP2013158337A5 JP2012033326A JP2012033326A JP2013158337A5 JP 2013158337 A5 JP2013158337 A5 JP 2013158337A5 JP 2012033326 A JP2012033326 A JP 2012033326A JP 2012033326 A JP2012033326 A JP 2012033326A JP 2013158337 A5 JP2013158337 A5 JP 2013158337A5
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nucleic acid
seq
amount
identification method
sample
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JP2012033326A
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JP2013158337A (en
JP6074561B2 (en
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Claims (8)

検体中の真菌の存在量の鑑別方法であって、
前記検体中の核酸を鋳型としてＰＣＲ反応を行い、配列番号１に示す塩基配列及び／またはそれと相同的な塩基配列を含む核酸を選択的に増幅する工程、及び
前記工程で産生された核酸量に基づいて、前記検体中に存在する菌量の多少を推定する工程を含むことを特徴とする鑑別方法。 A method for distinguishing the amount of fungi in a specimen ,
Performing a PCR reaction using the nucleic acid in the sample as a template, and selectively amplifying a nucleic acid comprising the base sequence shown in SEQ ID NO: 1 and / or a base sequence homologous thereto ; and
Based on the amount nucleic acid produced in the process, Kan-specific way to comprising the step of estimating some amount of bacteria present in the sample. 前記核酸はＤＮＡであることを特徴とする、請求項１に記載の鑑別方法。 Wherein said nucleic acid is DNA, Kan alternative method of claim 1. 前記ＰＣＲ反応に用いられるプライマーが、配列番号２に示される塩基配列又はそれと相同的な配列を有するオリゴヌクレオチド、及び配列番号３に示される塩基配列又はそれと相同的な配列を有するオリゴヌクレオチドであることを特徴とする、請求項１又は２に記載の鑑別方法。 Primers used for the PCR reaction is an oligonucleotide having the nucleotide sequence or homologous sequences thereto shown in oligonucleotide, and SEQ ID NO: 3 having the nucleotide sequence or homologous sequences thereto are shown in SEQ ID NO: 2 The identification method according to claim 1, wherein the identification method is provided. 既知の菌量の標準検体中の核酸を鋳型として検体と同条件でＰＣＲ反応を行って産生された核酸量を比較対象にして、前記推定する工程を行うことを特徴とする、請求項１〜３いずれか１項に記載の鑑別方法。 The estimation step is performed by comparing the amount of nucleic acid produced by performing a PCR reaction under the same conditions as the sample using a nucleic acid in a standard sample having a known amount of bacteria as a template . 3. The discrimination method according to any one of 3 items. 真菌の存在量を鑑別すべき検体は、動物より分離された皮膚乃至は爪であることを特徴とする、請求項１〜４いずれか１項に記載の鑑別方法。 5. The identification method according to claim 1, wherein the specimen to be identified for the abundance of the fungus is skin or nail separated from an animal. 前記動物は、真菌症の患者であることを特徴とする、請求項５に記載の鑑別方法。 The identification method according to claim 5, wherein the animal is a mycosis patient. 前記真菌はトリコフィトン・メンタグロファイテス又はトリコフィトン・ルブルムであることを特徴とする、請求項１〜６いずれか１項に記載の鑑別方法。 The identification method according to any one of claims 1 to 6, wherein the fungus is Trichophyton mentagrophytes or Trichophyton rubulum. 配列番号２もしくは配列番号３に示される塩基配列を有するオリゴヌクレオチド、又はそれらと相同的な配列を有するオリゴヌクレオチド。 Having a nucleotide sequence shown in SEQ ID NO: 2 or SEQ ID NO: 3 Luo Rigo nucleotides, or an oligonucleotide having a sequence homologous to those.
JP2012033326A 2012-02-02 2012-02-02 Method for distinguishing abundance of fungi and primers used therefor Active JP6074561B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2012033326A JP6074561B2 (en) 2012-02-02 2012-02-02 Method for distinguishing abundance of fungi and primers used therefor

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JP2012033326A JP6074561B2 (en) 2012-02-02 2012-02-02 Method for distinguishing abundance of fungi and primers used therefor

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JP2013158337A JP2013158337A (en) 2013-08-19
JP2013158337A5 true JP2013158337A5 (en) 2015-09-03
JP6074561B2 JP6074561B2 (en) 2017-02-08

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4936278B2 (en) * 2006-09-12 2012-05-23 良治 坪井 Diagnosis of onychomycosis

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