JP2013075880A - Methylmercaptan inhibitor - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an inhibitor for methylmercaptan becoming a problem in oral hygiene, and to provide an oral composition for preventing foul breath formulating the inhibitor.SOLUTION: The methylmercaptan inhibitor or the oral composition comprises one or more selected from the group consisting of an extract of olive leaves, an extract of fruits of Rubus idaeus, an extract of flowers of Caryophylli Flos, an extract of leaves of Argania spinosa, an extract of leaves of bilberry, an oil-soluble extract of licorice, an extract of leaves of cherry, an extract of buds of rose and an extract of bark of pine.

Description

  The present invention relates to an inhibitor of methyl mercaptan. More specifically, the present invention relates to a plant extract having an effect of suppressing the production of methyl mercaptan and / or removing the generated methyl mercaptan.

Methyl mercaptan is known as a so-called malodorous substance having a low odor threshold that gives off an odor even at low concentrations, but is also a substance that has various adverse effects on living bodies.
In the human body, it is known that methyl mercaptan is produced as a metabolite by bacteria assimilating sulfur-containing foods such as onions and cabbages, and proteins and peptides containing sulfur-containing amino acids. Especially in the oral cavity and pharynx, various tissues constitute complex forms, so food residues that can be sources of methyl mercaptan are likely to remain, and plaques and other bacteria that are difficult to physically and chemically remove. Since the breeding environment is easy to adjust, it is considered that methyl mercaptan is likely to be generated. When methyl mercaptan is generated in the oral cavity and pharynx, it causes the generation of bad breath.

  Therefore, attempts have been made to prevent the above-described phenomenon derived from methyl mercaptan by removing, deodorizing, and suppressing generation of methyl mercaptan. For example, from the viewpoint of removal and deodorization of methyl mercaptan, a method using a cyclodextrin derivative (Patent Document 1), an animal and plant extract (Patent Documents 2 and 3) molybdenum (Patent Document 4), and suppression of methyl mercaptan production In view of the above, a long-chain alkylylsarcosine salt having a specific metal as a base (Patent Document 5) or a combination of a legume extract and a specific compound (Patent Document 6) is one of the bacteria that produce methyl mercaptan. A method of acting on a genus Fusobacterium, plant extracts (Patent Documents 7 and 8), 3-chloro-DL-alanine (Non-Patent Document 1), zinc chloride (Non-Patent Document 2), epigallocatechin gallate (Non-Patent Documents) There is a method of inhibiting the activity of methioninase involved in the production of methyl mercaptan by adding a compound such as 3). However, none of these methods has been adequately solved because of the lack of effectiveness, the use of a sufficient amount, which causes difficulty in palatability and raises safety concerns. Therefore, it is desired to provide a safer and more effective method.

JP 2007-169228 A JP-A 64-26512 JP 2010-280591 A JP 2010-1111591 A JP-A-61-37720 JP 2002-114658 A JP 2008-31062 A JP 2010-265215 A

Yoshimura M, FEBS Letter 523, 119-122, 2002 Yamashita Y, J Dent Hlth 56, 335, 2006 Sakurai K, Y, J Dent Hlth 59, 391, 2009

  An object of the present invention is to provide an inhibitor of methyl mercaptan, which is a main cause of bad breath, and an oral composition for preventing bad breath containing the same.

  As a result of intensive studies in view of such circumstances, the present inventors have surprisingly found that olive leaf extract, raspberry fruit extract, clove flower extract, argania spinosa leaf extract, bilberry extract, oil-soluble licorice The present inventors have found that an extract, a cherry leaf extract, a rose bud extract and a pine bark extract have an excellent methyl mercaptan inhibitory effect and have completed the present invention.

That is, this invention provides the methyl mercaptan inhibitor of the following items 1-4 especially, and the composition for oral cavity which mix | blended it.
Item 1. From the group consisting of olive leaf extract, raspberry fruit extract, clove flower extract, argania spinosa leaf extract, bilberry extract, oil-soluble licorice extract, cherry leaf extract, rose bud extract and pine bark extract A methyl mercaptan inhibitor comprising one or more selected.
Item 2. An oral composition for preventing bad breath, comprising the methyl mercaptan inhibitor according to claim 1.
Item 3. The oral cavity composition for preventing bad breath according to claim 2, wherein the amount of the methyl mercaptan inhibitor is 0.001 to 10% by mass in terms of solid matter with respect to the total amount of the composition.
Item 4. The oral composition for preventing bad breath according to any one of claims 2 and 3, further comprising at least one selected from the group consisting of xylitol, maltitol, erythritol, and palatinit.

  The methyl mercaptan inhibitor of the present invention can prevent bad breath by suppressing the production of methyl mercaptan in the oral cavity. In addition, since it is a material with experience of eating, there is no concern about safety even if ingested over a long period of time, and therefore it can be applied to oral compositions for daily use in a preventive manner.

  In the present invention, the methyl mercaptan inhibitor is a plant extract obtained by extracting olive leaves, raspberries, clove flowers, Arganian spinosa leaves, bilberries, licorice, cherry leaves, rose buds and pine bark with a solvent. In the present specification, the blending ratio represents the blending mass ratio unless otherwise specified.

The olive leaf extract used in the present invention is obtained by extracting leaves of plants belonging to the genus Oleaceae. As a plant belonging to the genus Oliveaceae, specifically, olive (Olea
europaea Linne) and its congeners (for example, Olea welwitschii, Olea paniculata, etc.), and there are over 500 varieties. Typical examples of varieties include Neva di Bronco, Manzanillo, Picual, Hogi Blanco, Albequina, Catamaran, Colonneck, Pitchorine, Paragon, Wagga Verdal, Mission, Washington, West Australia Mission, South Australia Bendal, Azapa, Balnea, Cornicabra, Gordal, Frantoio, Retcino, Chippressino, Lucca, Ascorana Terena, Correggiora, Moloioro, Black Italian, Colatina, Helena, Rossiola, One Seven Seven, El Greco, Hardys Mammoth and so on. The olive leaf extract can be obtained by extracting the leaves of these genus Oligoceae plants using an organic solvent, water, or a mixture of an organic solvent and water. Among them, a mixed solution of water and ethanol is particularly preferable, and the mixing ratio of the mixed solution is, for example, preferably about 100: 1 to about 1: 200 by volume ratio of water: ethanol, more preferably about 20: 1 to 1:20, about Most preferred is 1: 9 to 1: 1. The temperature of the solvent during extraction may be in the range of about -4 ° C to about 200 ° C, but is preferably about 30 ° C to about 150 ° C, more preferably about 40 ° C to about 80 ° C. These olive leaf extracts are olive leaf extract BG (50% 1,3-butylene glycol aqueous solution: manufactured by Maruzen Pharmaceutical Co., Ltd.), olive leaf dried extract (powder: manufactured by Ask Pharmaceutical Co., Ltd.), olive leaf extract (Tama Biochemistry) Opiace (powder: Eisai Food Chemical Co., Ltd.), Oralis (powder: Eisai Food Chemical Co., Ltd.), olive leaf extract (30% ethanol aqueous solution: manufactured by Nippon Powder Chemical Co., Ltd.), olive leaf extract powder (powder: Japan) Powdered chemicals), olive leaf extract (Bioactives Japan) and the like.

The raspberry fruit extract used in the present invention is an extract of the fruit of a plant belonging to the genus Rosaceae. Specific examples of the raspberry genus plants include maple strawberries (Rubus palmatus var. Coptophyllus), red strawberry (Rubus trifidus), raspberries (Rubus idaeus), blackberries (Rubus fruticosus), and Fuyu strawberries (winter strawberries: Rubus buergeri Miq.
parvifolius L.), wedge strawberries (Kusana: Rubus)
hirsutus Thunb.). The raspberry fruit extract can be obtained by extracting the fruit of the plant belonging to the genus Rubiaceae, using an organic solvent, water, or a mixture of an organic solvent and water. Among them, a mixed solution of water and 1,3-butylene glycol or ethanol is particularly preferable, and a mixed solution of water and 1,3-butylene glycol is more preferable. These raspberry fruit extracts are raspberry BG (50% 1,3-butylene glycol aqueous solution: manufactured by Maruzen Pharmaceutical Co., Ltd.), raspberry BG100 (1,3-butylene glycol solution: manufactured by Maruzen Pharmaceutical Co., Ltd.), Falco Rex raspberry B (water / 1,3-butylene glycol mixed solution: manufactured by Ichimaru Falcos Co., Ltd.).

  The clove flower extract used in the present invention is obtained by extracting a clove flower or floret belonging to the genus Myrtaceae. Clove is also known as clove, butterfly (clover), or hyacinth. The clove flower extract is obtained by extracting a clove flower or floret with an organic solvent, water, or a mixture of an organic solvent and water. Among these, water or a mixed solution of water and ethanol is particularly preferable. These clove flower extracts are: Falco rex clove (water / ethanol mixture: manufactured by Ichimaru Falcos), clove extract-J (50% ethanol aqueous solution: manufactured by Maruzen Pharmaceutical), clove extract ET-50-D (water) / Ethanol mixed solution: manufactured by Koei Kogyo Co., Ltd.), etc.

  The Arganian spinosa leaf extract used in the present invention is obtained by extracting leaves of the Aceticaceae plant. Argania spinosa (Argania spinosa (L.) Skeels) is also known as the Argan tree. The Arganian spinosa leaf extract is obtained by extracting the Arganian spinosa leaf using an organic solvent, water or a mixture of an organic solvent and water. Among these, water or a mixed solution of water and ethanol is particularly preferable. These Arganian spinosa leaf extracts can be obtained as Arganyl LSF (aqueous solution: manufactured by LABORATOIRES SEROBIOLOGIQUES).

  The bilberry extract used in the present invention can be obtained by extracting the fruits and leaves of a plant of the genus Mytillus, using an organic solvent, water, or a mixture of an organic solvent and water. Plants of the genus Mytillus are called bilberries, cypresses, water berries, huckleberries, and the like. These bilberry extracts are Eco Farm Bilberry Leaf E (use site, leaf: water / ethanol mixture: Ichimaru Falcos), Cure Berry (use site, leaf: water / 1,3-butylene glycol mixture: Ichimaru Falcos) ), Bilberon (use part, fruit: soft extract: Tokiwa Plant Research Institute), VEGETOL BILBERRY MCF 1838 HYDRO (use part, fruit and leaf: propylene glycol aqueous solution: GATTEFOSS &Easy; made) .

  The oil-soluble licorice extract used in the present invention is an extract of leguminous licorice plants. As the plants of the licorice genus, there are G. uralensis, G. glabra, G. inflata, G. echinata L., G. pallidiflola MAX. Can be mentioned. Extraction sites are mainly roots, rhizomes, and leaves. The oil-soluble licorice extract can be obtained as licorice oil-based extract (manufactured by Tokiwa Plant Research Institute), oil-soluble licorice extract PT (manufactured by Maruzen Pharmaceutical Co., Ltd.) or the like.

  The cherry leaf extract used in the present invention is obtained by extracting leaves of a plant belonging to the genus Rosaceae using an organic solvent, water, or a mixture of an organic solvent and water. These cherry leaf extracts can be obtained as cherry extract B (1,3-butylene glycol aqueous solution: manufactured by Ichimaru Falcos).

  The rose bud extract used in the present invention can be obtained by extracting florets of a plant belonging to the family Rosaceae using an organic solvent, water, or a mixture of an organic solvent and water. These rose bud extracts can be obtained as rose butts extract powder MF (manufactured by Maruzen Pharmaceutical Co., Ltd.), rose bud extract powder (manufactured by Maruei Trading).

The pine bark extract used in the present invention is obtained by extracting the bark of Pinus pinaster from the Pinus pinaster using an organic solvent, water, or a mixture of organic solvent and water. , Also called the French cabbage bark extract. These pine bark extracts are OLIGOPIN (DRT), Flavangenol (Toyo Shinyaku), Pycnogenol (Horphag)
Research Inc.).

  The methyl mercaptan inhibitor of this invention is normally applied in an oral cavity by mix | blending with an oral composition. Although it does not specifically limit as an oral composition which can be mix | blended, Toothpaste, a gel agent, a liquid dentifrice, a mouthwash, a spray agent, a pasta agent, a soft paste (cream-form preparation), an ointment-form preparation , Tablets, granules, chewing gum, candy, lozenges, tablets, chewable tablets, granule capsules, beverages, etc. (dosage forms). Of these, toothpastes, gels, liquid dentifrices, mouthwashes, sprays, pasta agents, soft pastes (cream formulations), tablets, candy agents and the like are preferred. , Liquid dentifrice, mouthwash, spray, tablet, candy and the like are most preferred.

  The methyl mercaptan inhibitor is usually blended in an amount of 0.001 to 10% by mass with respect to these oral compositions in terms of solid matter. If the amount is less than 0.001% by mass, the desired effect cannot be expected. If the amount is more than 10% by mass, the flavor (such as astringency) derived from the extract is strongly felt, and the palatability is deteriorated.

  More optimal blending amounts differ depending on the type of methyl mercaptan inhibitor and the shape of the oral composition, and are specifically as follows. In addition, all compounding quantities are the mass% of solid content conversion with respect to the oral cavity composition whole quantity. In the case of olive leaf extract, toothpaste and gel are 0.01 to 1% by mass, mouthwash and liquid dentifrice are 0.001 to 0.2% by mass, spray, tablet and candy are 0. It is preferable to mix 1 to 10% by mass. In the case of bilberry extract, toothpaste, gel, spray, tablet, candy is 0.001-0.02% by mass, mouthwash and liquid dentifrice is 0.001-0.004% by mass It is preferable to do. In the case of Arganian spinosa leaf extract, 0.001-0.25 mass% for toothpaste, gel, spray, tablet, candy, 0.001-0.05 for mouthwash and liquid dentifrice. It is desirable to blend in mass%.

  In the composition for oral cavity which mix | blends the methyl mercaptan inhibitor of this invention, if it is a range which does not impair the effect of this invention, you may further mix | blend the component which can be normally mix | blended with an oral composition.

  As the surfactant, a nonionic surfactant, an anionic surfactant or an amphoteric surfactant can be blended. Specifically, nonionic surfactants include sugar fatty acid esters such as sucrose fatty acid esters, maltose fatty acid esters, and lactose fatty acid esters, fatty acid alkanolamides, sorbitan fatty acid esters, fatty acid monoglycerides, and polyoxyethylene addition coefficients of 4 to 15. A polyoxyethylene alkyl ether type having an alkyl group having 10 to 18 carbon atoms or a polyoxyethylene alkyl phenyl ether having a polyoxyethylene addition coefficient of 10 to 18 and an alkyl group having 9 carbon atoms, diethyl sebacate, poly Oxyethylene hydrogenated castor oil, polyoxyethylene sorbitan fatty acid ester, polyoxyethylene glycerin fatty acid ester, polyoxyethylene sorbit fatty acid ester, polyethylene glycol fatty acid ester, polyester Renranorin, polyethylene sterols, polyethylene lanolin alcohols, alkyl glucosides, polyoxyethylene polyoxypropylene block copolymers, and the like. Anionic surfactants include sulfate esters such as sodium lauryl sulfate and polyoxyethylene lauryl ether sulfate, sodium lauryl sulfosuccinate, sulfosuccinates such as sodium polyoxyethylene lauryl ether sulfosuccinate, cocoyl sarcosine sodium, lauroylmethylalanine Examples include acyl amino acid salts such as sodium, cocoyl methyl taurine sodium and the like. Zwitterionic surfactants include betaine acetate type activators such as lauryl dimethylaminoacetic acid betaine, coconut oil fatty acid amidopropyldimethylaminoacetic acid betaine, and imidazoline type such as N-cocoyl-N-carboxymethyl-N-hydroxyethylethylenediamine sodium. Examples include activators and amino acid type activators such as N-lauryldiaminoethylglycine. These surfactants can be used alone or in combination of two or more.

  As a flavoring agent, for example, menthol, carvone, methyl salicylate, vanillin, benzyl succinate, methyl eugenol, anethole, limonene, ocimene, n-decyl alcohol, methyl acetate, citronenyl acetate, cineol, ethyl linalool, vanillin, thyme , Nutmeg, spearmint oil, peppermint oil, lemon oil, orange oil, sage oil, rosemary oil, cinnamon oil, perilla oil, winter green oil, clove oil, eucalyptus oil, pimento oil, tea tree oil, tabana oil, star Examples include anise oil, fennel oil, diatom oil, and basil oil. These fragrance | flavors can be used individually or in combination of 2 or more types.

  Examples of the abrasive include abrasive silica such as abrasive precipitated silica and abrasive gel silica, calcium hydrogenphosphate dihydrate and anhydride, calcium phosphate, tertiary calcium phosphate, tertiary magnesium phosphate, calcium pyrophosphate, hydroxyapatite Insoluble sodium metaphosphate, aluminum silicate, zirconium silicate, calcium silicate, calcium carbonate, magnesium carbonate, alumina, aluminum hydroxide, calcium sulfate, polymethyl methacrylate, pumice, bentonite, synthetic resin, etc. It is done. These abrasive | polishing agents can be used individually or in combination of 2 or more types.

  Examples of the binder include carboxymethyl cellulose sodium, carboxymethyl ethyl cellulose salt, cellulose derivatives such as hydroxyethyl cellulose, hydroxypropyl cellulose, hydroxypropyl methyl cellulose, microbial polymer such as xanthan gum, gellan gum, tragacanth gum, karaya gum, arabiya gum, carrageenan, dextrin. Natural polymers such as natural rubbers, synthetic polymers such as polyvinyl alcohol and polyvinyl pyrrolidone, thickening silica, inorganic binders such as bee gum, O- [2-hydroxy-3- (trimethylammonio) propyl] chloride Cationic binders such as hydroxyethyl cellulose can be mentioned. These binders can be used alone or in combination of two or more.

  Examples of sweeteners include saccharin, saccharin sodium, acesulfame potassium, stevia extract, stevioside, neohesperidyl dihydrochalcone, glycyrrhizin, perilartin, saumatine, aspartylphenylalanine methyl ester, methoxycinnamic aldehyde, sucralose, palatinose, palatinit, erythritol. , Maltitol, xylitol, lactitol and the like. These sweeteners can be used alone or in combination of two or more.

  As the wetting agent, for example, glycerin, ethylene glycol, propylene glycol, 1,3-propanediol, 1,3-butylene glycol, sorbit, polyethylene glycol, polypropylene glycol and the like may be used alone or in combination of two or more. it can.

  Examples of the preservative include parabens such as methylparaben and propylparaben, and benzoates such as sodium benzoate. These preservatives can be used alone or in combination of two or more.

  Examples of pH adjusters include citric acid, phosphoric acid, malic acid, gluconic acid, maleic acid, aspartic acid, succinic acid, glucuronic acid, fumaric acid, glutamic acid, adipic acid, and salts thereof, hydrochloric acid, sodium hydroxide , Potassium hydroxide, sodium silicate and the like. These components can be contained in the oral composition of the present invention alone or in combination of two or more. In addition, as long as the composition for oral cavity of this invention is a range which can be used in an oral cavity, the pH will not be restrict | limited, However, Usually, pH is 3.0-10.5, Preferably it is pH 5.5-8.0.

  As a medicinal component, in addition to cetylpyridinium chloride as a bactericidal agent, for example, cationic bactericides such as chlorhexidine hydrochloride, chlorhexidine gluconate, benzethonium chloride, benzalkonium chloride; amphoteric bactericides such as dodecyldiaminoethylglycine; isopropylmethylphenol, Nonionic fungicides such as triclosan; Enzymes such as dextranase, amylase, protease, mutanase, lysozyme, lytic enzyme (lytechenzyme); Glycyrrhizic acid salts such as glycyrrhetinic acid and dipotassium glycyrrhizinate as anti-inflammatory agents; Nicotinic acid or tocopherol acetate, etc .; Tranexamic acid, epsilon aminocaproic acid, etc. as antiplasmin agent; Ascorbic acid, etc. as bleeding remedy; Fluorine compounds such as sodium fluoride as remineralizing agents; plant extracts extracted with water-soluble solvents, chlorophyll, sodium chloride, caropeptides, zinc chloride, hinokitiol, etc. It can mix | blend combining a seed | species or more.

  Among the above components, the oral composition containing xylitol, maltitol, erythritol, and palatinit suppresses the astringency and bitterness of the methyl mercaptan inhibitor contained in the oral composition, and the palatability of the oral composition Is preferable in order to increase. In particular, effects are easily obtained in liquid dentifrices, mouthwashes, and sprays that tend to feel astringency and bitterness, and it is preferably blended in an amount of 1 to 10% by weight, preferably 5 to 10% by weight based on the total amount of the composition. Is more preferable. In addition, it is not preferable to add more than 10% by mass since the possibility of precipitation of crystals such as xylitol from the composition adhering to the discharge port of the container or the inside of the cap increases when the oral composition is used.

  Hereinafter, the present invention will be specifically described, but the present invention is not limited to the following examples. In the following, “%” means “mass%” unless otherwise specified.

Evaluation of removal effect of methyl mercaptan The removal effect of methyl mercaptan was measured according to the following method.
To a glass bottle of 15 mL capacity, 0.1 mL of a 0.1 M phosphate buffer (pH 7.5) 1.5 mL test solution was added and stirred for 5 seconds with a vortex mixer. 0.5 mL of a 1 μg / mL methyl mercaptan solution was added, and the mixture was stirred for 5 seconds with a vortex mixer, and then kept at a constant temperature layer of 37 ° C. for 6 minutes. After injecting 1 mL of air with a gas tight syringe and stirring for 5 seconds with a vortex mixer, 1 mL of gas was extracted from the head space of the glass bottle and injected into a gas chromatograph to measure the amount of methyl mercaptan. The analysis conditions are shown below. The methyl mercaptan removal rate is defined as follows: Ki is the amount of methyl mercaptan when the test solution is added, and K0 is the amount of methyl mercaptan when ethanol: water = 4: 1 (volume ratio) is used instead of the test solution. Calculated according to the formula. In addition, ethanol: distilled water = 4: 1 (volume ratio) was used for the blank. The obtained results are shown in Table 1.

Column: SUPELCO, Supel-QPLOT 0.53mm × 30m
Column temperature: 50 ° C hold 1min 50-130 ° C 10 ° C / min
Detector: Flame photometric detector Injection volume: 1 mL
Equipment used: Auto system XL

Adsorption removal rate (%) = [(K0−Ki) / K0] × 100

The test solution and 1 μg / mL methyl mercaptan solution (1 μg / mL MeSH solution) used for evaluation were prepared by the following method.

Preparation of specimen: Each specimen is dissolved or diluted with a solution mixed with ethanol: distilled water = 4: 1 (volume ratio), and the solid equivalent concentration contained in the specimen is 0.1% by mass. It was prepared as follows.
Preparation of 1 μg / mL MeSH solution: Ethanol was added to 200 mL to 2 mL of a standard solution of 1 μg / μL MeSH (for Wodor Pure Chemicals test for malodorous substance). Furthermore, distilled water was added to 2 mL of the solution to adjust the total volume to 20 mL, and a 1 μg / mL MeSH solution was obtained.

The following samples were used.
Olive leaf extract: Opiace (powder: Eisai Food Chemical Co., Ltd.)
Raspberry fruit extract: Raspberry extract BG (Maruzen Pharmaceutical Co., Ltd.)
Clove flower extract: Falco Rex clove (water / ethanol mixture: Ichimaru Falcos)
Arganian spinosa leaf extract: Arganyl LSF (aqueous solution: LABORATOIRES SEROBIOLOGIQUES)
Bilberry extract: Eco Farm Bilberry Leaf E (use site, leaf: water / ethanol mixture: Ichimaru Falcos)
Oil-soluble licorice extract: Oil-soluble licorice extract PT (manufactured by Maruzen Pharmaceutical Co., Ltd.)
Sakura leaf extract: Sakura extract B (1,3-butylene glycol aqueous solution: manufactured by Ichimaru Falcos)
Rose bud extract: Rose butts extract powder MF (Maruzen Pharmaceutical Co., Ltd.)
Pine bark extract: Pycnogenol (Horphag Research)
Rice bran extract: Purple brown rice nuka extract BG (manufactured by Maruzen Pharmaceutical Co., Ltd.)
Turmeric extract: Falco Rex Turmeric B (Ichimaru Falcos)
Apple fruit extract: Falco Rex Apple B (Ichimaru Falcos)

  As shown in Table 1, olive leaf extract, raspberry fruit extract, clove flower extract, Arganian spinosa leaf extract, bilberry extract, oil-soluble licorice extract, cherry leaf extract, rose bud extract, pine The bark extract was found to have an excellent methyl mercaptan removal effect. In particular, olive leaf extract, raspberry fruit extract, clove flower extract, argania spinosa leaf extract, bilberry extract show high effects, among them olive leaf extract, raspberry fruit extract, clove flower extract Was found to show a remarkable effect. On the other hand, it was found that the rice bran extract, turmeric extract, and apple fruit extract do not have a large removal effect.

Evaluation of Methyl Mercaptan Generation Suppression Effect The methyl mercaptan generation suppression effect was measured according to the following method.
After adding 770 μL of the salt buffer, 100 μL of the test solution, and 100 μL of the bacterial suspension to a glass bottle of 15 mL volume, and shaking lightly by hand, 30 μL of 33 mM L-methionine salt buffer was added, followed by incubation at 37 ° C. for 90 minutes. Immediately after culturing, 500 μL of 3M phosphoric acid aqueous solution was added and stirred, and allowed to stand for 10 minutes. Then, 1 mL of gas was sampled from the head space of the glass bottle, and injected into a gas chromatograph to quantify methyl mercaptan. The analysis conditions are the same as those described in the evaluation of the removal effect of methyl mercaptan. The methyl mercaptan generation inhibition rate (%) is defined as the amount of methyl mercaptan when the test solution is added, Ki,
The amount of methyl mercaptan when a salt buffer was added instead of the test solution was defined as K0 and calculated using the following formula. As a blank, 40 mM potassium phosphate buffer (pH 7.7) to which 50 mM sodium chloride was added was used. The obtained results are shown in Table 2.

Occurrence suppression rate (%) = [(K0−Ki) / K0] × 100

The bacteria, reagents, test solution, culture medium, and bacterial suspension used in the test were as follows.
Bacteria used: Porphyromonas gingivalis strain W83 (hereinafter sometimes abbreviated as Pg)
Salt buffer solution: 40 mM aqueous potassium dihydrogen phosphate solution was added to 40 mM aqueous dipotassium hydrogen phosphate solution to adjust the pH to 7.7. 1 L of the solution was taken and 2.92 g of sodium chloride was added and heat sterilized.
Test solution: Each test sample was prepared using a salt buffer so as to have a prescribed concentration in terms of solid matter.
Medium: First, 0.25 g of hemin was dissolved in 5 mL of 1N sodium hydroxide, and 20 mL of distilled water was further added to make solution A. A solution B was prepared by dissolving 0.025 g of menadione in 25 mL of 99% ethyl alcohol. The solutions A and B were mixed to prepare a hemin and menadione solution. The medium used was an autoclaved solution obtained by dissolving 40 g of TSB, 1 mL of hemin, menadione solution and 1 g of Yeast product in 0.9 L of distilled water. This medium is hereinafter referred to as “TSB + h, m, y”.
Bacterial suspension: Pg was inoculated into TSB + h, m, y 10 ml, anaerobically cultured at 37 ° C. for 24-48 hr, and then this culture solution was added to fresh TSB + h, m, y 100 ml, and at 37 ° C., 24-48 hr. Anaerobic culture was performed. This bacterial culture was centrifuged at 8000 × g for 10 minutes, washed with a salt buffer, suspended in a salt buffer, and adjusted to about 0.3 at an OD550 nm of 33 mM L-methionine salt buffer solution: L- Methionine was prepared using the salt buffer solution so as to have a final concentration of 33 mM and sterilized by filtration.
3M phosphoric acid aqueous solution: A phosphoric acid and distilled water were used to prepare a final concentration of 3M.

The following samples were used.
Olive leaf extract: Opiace (powder: Eisai Food Chemical Co., Ltd.)
Raspberry fruit extract: Raspberry extract BG (Maruzen Pharmaceutical Co., Ltd.)
Clove flower extract: Falco Rex clove (water / ethanol mixture: Ichimaru Falcos)
Arganian spinosa leaf extract: Arganyl LSF (aqueous solution: LABORATOIRES SEROBIOLOGIQUES)
Bilberry extract: Eco Farm Bilberry Leaf E (use site, leaf: water / ethanol mixture: Ichimaru Falcos)
Oil-soluble licorice extract: Oil-soluble licorice extract PT (manufactured by Maruzen Pharmaceutical Co., Ltd.)
Sakura leaf extract: Sakura extract B (1,3-butylene glycol aqueous solution: manufactured by Ichimaru Falcos)
Novara fruit extract: Falco Rex Novara B (manufactured by Ichimaru Falcos)
Hawthorn Extract: Falco Rex Hawthorn B (manufactured by Ichimaru Falcos)

  As shown in Table 2, excellent methyl mercaptan generation in olive leaf extract, raspberry fruit extract, clove flower extract, Arganian spinosa leaf extract, bilberry extract, oil-soluble licorice extract, and cherry leaf extract It was found that there is a suppression effect. In particular, it was found that when 0.001% by mass or more is blended, an inhibitory effect of 50% or more is obtained, and when 0.01% or more is blended, a suppressive effect of 75% or more is obtained. On the other hand, it was found that nocturnal fruit extract or hawthorn extract could not obtain any inhibitory effect despite the blending of 0.01% by mass or more.

Evaluation of Bitterness / Astringency Suppression Effect According to the following method, the bitterness / astringency suppression effect of an oral composition containing a methyl mercaptan inhibitor was evaluated.
Examples 1 to 6 and Comparative Example 1 produced by a conventional method were subjected to absolute evaluation using five grades based on the following criteria using five specialist panels. For the evaluation, 10 ml of each subject was taken and contained in the mouth for about 20 seconds. The obtained evaluation points were totaled, the average value of the evaluation points was calculated for each evaluation item, and the results are shown in Table 3.
Evaluation point 5: Feel weak
4: Feel somewhat weak
3: Neither can be said
2: I feel a little stronger
1: Feel strong

  As shown in Table 3, when 1 to 10% by mass of xylitol, maltitol, and erythritol is added to the total amount of the oral composition, the bitterness and astringency of the oral composition containing the olive leaf extract are remarkably suppressed. I understood it.

  Hereinafter, although the prescription of the Example of the composition for oral cavity which mix | blended the methyl mercaptan inhibitor which concerns on this invention is given, this invention is not limited to the following prescription.

Formulation Example 1
A toothpaste was prepared according to a conventional method.
Component amount
Cetylpyridinium chloride 0.05
Polyoxyethylene (40) hydrogenated castor oil 0.5
Argania spinosa leaf extract 0.5
Abrasive silica 18.0
Thickening silica 3.0
Sorbitol 40.0
Xylitol 5.0
Polyethylene glycol 400 3.0
Fragrance 1.0
Saccharin sodium 0.2
Sodium carboxymethylcellulose 0.4
Sodium fluoride 0.2
Isopropyl methylphenol 0.05
pH adjusting agent
Purified water balance
Total 100

Formulation example 2
A gel was prepared according to a conventional method.
Component amount
Bilberry extract 1.0
Glycerin 10.0
Propylene glycol 9.0
Maltitol 1.0
Hydroxyethyl cellulose 1.0
Dipotassium glycyrrhizinate 0.05
Polyoxyethylene (60E.O.) hydrogenated castor oil 0.4
Citric acid 0.01
Trisodium citrate 0.1
Fragrance 0.4
Methyl paraoxybenzoate 0.1
Sucralose 0.02
Purified water balance
Total 100

Formulation Example 3
A liquid dentifrice was prepared according to a conventional method.
Component amount
Argania spinosa leaf extract 0.02
Glycerin 10.0
Ethanol 10.0
Xylitol 7.0
Polyoxyethylene (60E.O.) hydrogenated castor oil 0.4
Cetylpyridinium chloride 0.3
Tranexamic acid 0.05
Citric acid 0.01
Trisodium citrate 0.1
Methyl paraoxybenzoate 0.1
Fragrance 0.2
Purified water balance
Total 100

Formulation Example 4
A mouthwash was prepared according to a conventional method.
Component amount
Olive leaf extract 0.001
Maltitol 10.0
Glycerin 5.0
Polyoxyethylene (60E.O.) hydrogenated castor oil 0.5
Cetylpyridinium chloride 0.1
Sodium citrate 0.1
Saccharin 0.05
Fragrance 0.2
Purified water balance
Total 100

Formulation Example 5
A mouse spray was prepared according to a conventional method.
Component amount
Olive leaf extract 1.0
Ethanol 30.0
Glycerin 15.0
Polyoxyethylene (60E.O.) hydrogenated castor oil 1.5
Menthol 1.0
Saccharin 0.1
Sodium citrate 0.2
Purified water balance
Total 100

Formulation Example 6
A tablet was prepared according to a conventional method.
Component amount
Palatinit 35.0
Sucrose fatty acid ester 2.0
Olive leaf extract 2.0
Sucralose 0.1
Acesulfame potassium 0.05
Fragrance 1.5
Maltitol balance
Total 100

Formulation Example 7
Candy was prepared according to a conventional method.
Component amount
Bilberry extract 2.0
Maltitol 10.0
Aspartame 0.1
Fragrance 0.2
Palatinit rest
Total 100

Formulation Example 8
Granule capsules were prepared according to a conventional method.
Granule capsules were prepared by encapsulating 60 parts by weight of the solution in the capsule with 40 parts by weight of the inner capsule film made of gelatin and sorbitol, and sugar coating with 110 parts by weight of the outer capsule film made of sugar.

Solution in capsule
Component amount
Olive leaf extract 10.0
Vitamin C 7.2
Vitamin E 2.4
Glycerin fatty acid ester 1.0
Safflower oil balance
Total 60

Inner capsule film
Component amount
Gelatin 36.0
Sorbitol balance
Total 40

Outer capsule coating
Component amount
Eggshell calcium 1.0
Gum arabic 0.6
Shellac 0.3
Gelatin 0.2
Carbana wax 0.1
Aspartame 0.1
Fragrance 0.4
Palatinit rest
Total 110

Formulation Example 9
An oral pasta preparation was prepared according to a conventional method.
Component amount
Glycerin 20.0
Liquid paraffin 11.0
Cetanol 9.0
Polyoxyethylene sorbitan monostearate 4.0
Cetyltrimethylammonium chloride 2.0
Lecithin 2.0
Sorbitan monopalmitate 1.0
Tocopherol nicotinate 1.0
Bilberry extract 1.0
Sodium lauryl sulfate 0.3
Benzethonium chloride 0.2
Cetylbenzethonium chloride 0.1
Methyl salicylate 0.15
Cetylpyridinium chloride 0.05
Saccharin 0.05
Fragrance 0.3
Purified water balance
Total 100

Formulation Example 10
Chewing gum was prepared according to a conventional method.
Component amount
Xylitol 58.0
Maltitol 8.0
Calcium carbonate 7.0
Fragrance 3.0
Argania spinosa leaf extract 1.0
The rest of the gum base
Total 100

Formulation Example 11
Chewable tablets were prepared according to a conventional method.
Component amount
Bilberry extract 2.0
Gum arabic 1.2
Sucrose fatty acid ester 1.8
Fragrance 3.0
Palatinit rest
Total 100

Claims (4)

  From the group consisting of olive leaf extract, raspberry fruit extract, clove flower extract, argania spinosa leaf extract, bilberry extract, oil-soluble licorice extract, cherry leaf extract, rose bud extract and pine bark extract A methyl mercaptan inhibitor comprising one or more selected.   An oral composition for preventing bad breath, comprising the methyl mercaptan inhibitor according to claim 1.   The oral cavity composition for preventing bad breath according to claim 2, wherein the amount of the methyl mercaptan inhibitor is 0.001 to 10% by mass in terms of solid matter with respect to the total amount of the composition.   The oral composition for preventing bad breath according to any one of claims 2 and 3, further comprising at least one selected from the group consisting of xylitol, maltitol, erythritol, and palatinit.