JP2016147869A - Composition for oral cavity for suppressing methyl mercaptan generation - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a composition for oral cavity for suppressing generation of methyl mercaptan being a problem on oral hygiene.SOLUTION: There is provided any one composition for oral cavity for suppressing methyl mercaptan generation which is selected from a chewing gum agent, candy agent, troche agent, tablet agent, chewable tablet agent and beverage in which olive leaf extract and one or more types of sugar alcohol selected from xylitol, maltitol, erythritol, and palatinit are blended as active ingredients for suppressing generation of methyl mercaptan. In the composition for oral cavity for suppressing methyl mercaptan generation, it is preferable that the olive leaf extract is 0.0001 to 10 mass% to the total amount of the composition in terms of solid content.SELECTED DRAWING: None

Description

  The present invention relates to a composition for oral cavity for suppressing generation of methyl mercaptan. More specifically, an oral composition containing a plant extract having an effect of suppressing the production of methyl mercaptan, and more specifically, an oral cavity selected from chewing gum, candy, troche, tablet, chewable tablet and beverage The present invention relates to a composition for use.

Methyl mercaptan is known as a so-called malodorous substance having a low odor threshold that gives off an odor even at low concentrations, but is also a substance that has various adverse effects on living bodies.
In the human body, it is known that methyl mercaptan is produced as a metabolite by bacteria assimilating sulfur-containing foods such as onions and cabbages, and proteins and peptides containing sulfur-containing amino acids. Especially in the oral cavity and pharynx, various tissues constitute complex forms, so food residues that can be sources of methyl mercaptan are likely to remain, and plaques and other bacteria that are difficult to physically and chemically remove. Since the breeding environment is easy to adjust, it is considered that methyl mercaptan is likely to be generated. When methyl mercaptan is generated in the oral cavity and pharynx, it causes the generation of bad breath.

  Therefore, attempts have been made to prevent the above-described phenomenon derived from methyl mercaptan by removing, deodorizing, and suppressing generation of methyl mercaptan. For example, from the viewpoint of removal and deodorization of methyl mercaptan, a method using a cyclodextrin derivative (Patent Document 1), an animal and plant extract (Patent Documents 2 and 3) molybdenum (Patent Document 4), and suppression of methyl mercaptan production In view of the above, a long-chain alkylylsarcosine salt having a specific metal as a base (Patent Document 5) or a combination of a legume extract and a specific compound (Patent Document 6) is one of the bacteria that produce methyl mercaptan. A method of acting on a genus Fusobacterium, plant extracts (Patent Documents 7 and 8), 3-chloro-DL-alanine (Non-Patent Document 1), zinc chloride (Non-Patent Document 2), epigallocatechin gallate (Non-Patent Documents) There is a method of inhibiting the activity of methioninase involved in the production of methyl mercaptan by adding a compound such as 3). However, none of these methods has been adequately solved because of the lack of effectiveness, the use of a sufficient amount, which causes difficulty in palatability and raises safety concerns. Therefore, it is desired to provide a safer and more effective method.

JP 2007-169228 A JP-A 64-26512 JP 2010-280591 A JP 2010-1111591 A JP-A-61-37720 JP 2002-114658 A JP 2008-31062 A JP 2010-265215 A

Yoshimura M, FEBS Letter 523, 119-122, 2002 Yamashita Y, J Dent Hlth 56, 335, 2006 Sakurai K, Y, J Dent Hlth 59, 391, 2009

  This invention makes it a subject to provide the composition for oral cavity for generation | occurrence | production suppression of the methyl mercaptan which becomes the main cause of bad breath.

  As a result of intensive studies in view of such circumstances, the present inventors have surprisingly found that an excellent effect of suppressing the generation of methyl mercaptan exists in the olive leaf extract, and have completed the present invention.

That is, this invention provides the composition for oral cavity for especially the generation | occurrence | production suppression of the methyl mercaptan of the following items 1-4.
Item 1. An oral composition for suppressing methyl mercaptan generation containing an olive leaf extract as an active ingredient.
Item 2. Item 2. The oral cavity for suppressing the occurrence of methyl mercaptan according to Item 1, which is a composition for oral cavity selected from the group consisting of chewing gum, candy, troche, tablet, chewable tablet and beverage. Composition.
Item 3. Item 3. The oral use for suppressing methyl mercaptan generation according to any one of Items 1 or 2, wherein the olive leaf extract is 0.001 to 10% by mass in terms of solid matter with respect to the total amount of the composition. Composition.
Item 4. Item 4. The oral composition for suppressing methyl mercaptan generation according to any one of Items 2 and 3, further comprising at least one selected from the group consisting of xylitol, maltitol, erythritol, and palatinit.

  The composition for oral cavity for suppressing the production of methyl mercaptan according to the present invention can prevent bad breath by suppressing the production of methyl mercaptan in the oral cavity. In addition, since it is a material with experience of eating, there is no concern about safety even if ingested over a long period of time, and therefore it can be applied to oral compositions for daily use in a preventive manner.

  The methyl mercaptan generation inhibitory component of the present invention is a plant extract obtained by extracting olive leaves with a solvent. In the present specification, the blending ratio represents the blending mass ratio unless otherwise specified.

The olive leaf extract used in the present invention is obtained by extracting leaves of plants belonging to the genus Oleaceae. Specific examples of the plants belonging to the genus Oleaceae include olives (Olea europaea Linne) and the same species (for example, Olea welwitschii, Olea paniculata, etc.), and there are over 500 varieties, Representative examples of this include, for example, Neva di Bronco, Manzanillo, Picual, Hogi Blanco, Albequina, Catamara, Colonneck, Pitchorine, Paragon, Wagga Verdal, Mission, Washington, West Australia Mission, South Australia Bendal, Azapa, Balnea, Cornicabra, Gordar , Frantoio, Leccino, Cipressino, Lucca, Ascorana Telena, Correggiora, Moloio, Black Italian, Colatina, Helena, Rociola, One Seven Seven, El Greco, Hardiz Mammoth And the like. The olive leaf extract can be obtained by extracting the leaves of these genus Oligoceae plants using an organic solvent, water, or a mixture of an organic solvent and water. Among them, a mixed solution of water and ethanol is particularly preferable, and the mixing ratio of the mixed solution is, for example, preferably about 100: 1 to about 1: 200 by volume ratio of water: ethanol, more preferably about 20: 1 to 1:20, about Most preferred is 1: 9 to 1: 1. The temperature of the solvent during extraction may be in the range of about -4 ° C to about 200 ° C, but is preferably about 30 ° C to about 150 ° C, more preferably about 40 ° C to about 80 ° C. These olive leaf extracts are olive leaf extract BG (50% 1,3-butylene glycol aqueous solution: manufactured by Maruzen Pharmaceutical Co., Ltd.), olive leaf dried extract (powder: manufactured by Ask Pharmaceutical Co., Ltd.), olive leaf extract (Tama Biochemistry) Opiace (powder: Eisai Food Chemical Co., Ltd.), Oralis (powder: Eisai Food Chemical Co., Ltd.), olive leaf extract (30% ethanol aqueous solution: manufactured by Nippon Powder Chemical Co., Ltd.), olive leaf extract powder (powder: Japan) Powdered chemicals), olive leaf extract (Bioactives Japan) and the like.

  The methyl mercaptan generation inhibiting component of the present invention is usually applied to the oral cavity by blending it into the oral composition. Although it does not specifically limit as a composition for oral cavity which can be mix | blended, It can use as forms (dosage forms), such as a chewing gum agent, a candy agent, a troche agent, a tablet agent, a chewable tablet agent, a granule capsule, and a drink. . Of these, forms such as tablets and candy are preferred, and forms such as tablets and candy are most preferred.

  The methyl mercaptan generation-suppressing component is usually blended in an amount of 0.001 to 10% by mass with respect to these oral compositions in terms of solid matter. If the amount is less than 0.001% by mass, the desired effect cannot be expected. If the amount is more than 10% by mass, the flavor (such as astringency) derived from the extract is strongly felt, and the palatability is deteriorated.

  The more optimal blending amount varies depending on the dosage form of the oral composition, and is specifically as follows. In addition, all compounding quantities are the mass% of solid content conversion with respect to the oral cavity composition whole quantity. In the case of an olive leaf extract, it is preferable to add 0.1 to 10% by mass of the tablet and candy.

  The oral composition containing the methyl mercaptan generation-suppressing component of the present invention may be further blended with components that can be usually blended with the oral composition as long as the effects of the present invention are not impaired.

  As the surfactant, a nonionic surfactant, an anionic surfactant or an amphoteric surfactant can be blended. Specifically, nonionic surfactants include sugar fatty acid esters such as sucrose fatty acid esters, maltose fatty acid esters, and lactose fatty acid esters, fatty acid alkanolamides, sorbitan fatty acid esters, fatty acid monoglycerides, and polyoxyethylene addition coefficients of 4 to 15. A polyoxyethylene alkyl ether type having an alkyl group having 10 to 18 carbon atoms or a polyoxyethylene alkyl phenyl ether having a polyoxyethylene addition coefficient of 10 to 18 and an alkyl group having 9 carbon atoms, diethyl sebacate, poly Oxyethylene hydrogenated castor oil, polyoxyethylene sorbitan fatty acid ester, polyoxyethylene glycerin fatty acid ester, polyoxyethylene sorbit fatty acid ester, polyethylene glycol fatty acid ester, polyester Renranorin, polyethylene sterols, polyethylene lanolin alcohols, alkyl glucosides, polyoxyethylene polyoxypropylene block copolymers, and the like. Anionic surfactants include sulfate esters such as sodium lauryl sulfate and polyoxyethylene lauryl ether sulfate, sodium lauryl sulfosuccinate, sulfosuccinates such as sodium polyoxyethylene lauryl ether sulfosuccinate, cocoyl sarcosine sodium, lauroylmethylalanine Examples include acyl amino acid salts such as sodium, cocoyl methyl taurine sodium and the like. Zwitterionic surfactants include betaine acetate type activators such as lauryl dimethylaminoacetic acid betaine, coconut oil fatty acid amidopropyldimethylaminoacetic acid betaine, and imidazoline type such as N-cocoyl-N-carboxymethyl-N-hydroxyethylethylenediamine sodium. Examples include activators and amino acid type activators such as N-lauryldiaminoethylglycine. These surfactants can be used alone or in combination of two or more.

  As a flavoring agent, for example, menthol, carvone, methyl salicylate, vanillin, benzyl succinate, methyl eugenol, anethole, limonene, ocimene, n-decyl alcohol, methyl acetate, citronenyl acetate, cineol, ethyl linalool, vanillin, thyme , Nutmeg, spearmint oil, peppermint oil, lemon oil, orange oil, sage oil, rosemary oil, cinnamon oil, perilla oil, winter green oil, clove oil, eucalyptus oil, pimento oil, tea tree oil, tabana oil, star Examples include anise oil, fennel oil, diatom oil, and basil oil. These fragrance | flavors can be used individually or in combination of 2 or more types.

  Examples of the abrasive include abrasive silica such as abrasive precipitated silica and abrasive gel silica, calcium hydrogenphosphate dihydrate and anhydride, calcium phosphate, tertiary calcium phosphate, tertiary magnesium phosphate, calcium pyrophosphate, hydroxyapatite Insoluble sodium metaphosphate, aluminum silicate, zirconium silicate, calcium silicate, calcium carbonate, magnesium carbonate, alumina, aluminum hydroxide, calcium sulfate, polymethyl methacrylate, pumice, bentonite, synthetic resin, etc. It is done. These abrasive | polishing agents can be used individually or in combination of 2 or more types.

  Examples of the binder include carboxymethyl cellulose sodium, carboxymethyl ethyl cellulose salt, cellulose derivatives such as hydroxyethyl cellulose, hydroxypropyl cellulose, hydroxypropyl methyl cellulose, microbial polymer such as xanthan gum, gellan gum, tragacanth gum, karaya gum, arabiya gum, carrageenan, dextrin. Natural polymers such as natural rubbers, synthetic polymers such as polyvinyl alcohol and polyvinyl pyrrolidone, thickening silica, inorganic binders such as bee gum, O- [2-hydroxy-3- (trimethylammonio) propyl] chloride Cationic binders such as hydroxyethyl cellulose can be mentioned. These binders can be used alone or in combination of two or more.

  Examples of sweeteners include saccharin, saccharin sodium, acesulfame potassium, stevia extract, stevioside, neohesperidyl dihydrochalcone, glycyrrhizin, perilartin, saumatine, aspartylphenylalanine methyl ester, methoxycinnamic aldehyde, sucralose, palatinose, palatinit, erythritol. , Maltitol, xylitol, lactitol and the like. These sweeteners can be used alone or in combination of two or more.

  As the wetting agent, for example, glycerin, ethylene glycol, propylene glycol, 1,3-propanediol, 1,3-butylene glycol, sorbit, polyethylene glycol, polypropylene glycol and the like may be used alone or in combination of two or more. it can.

  Examples of the preservative include parabens such as methylparaben and propylparaben, and benzoates such as sodium benzoate. These preservatives can be used alone or in combination of two or more.

  Examples of pH adjusters include citric acid, phosphoric acid, malic acid, gluconic acid, maleic acid, aspartic acid, succinic acid, glucuronic acid, fumaric acid, glutamic acid, adipic acid, and salts thereof, hydrochloric acid, sodium hydroxide , Potassium hydroxide, sodium silicate and the like. These components can be contained in the oral composition of the present invention alone or in combination of two or more. In addition, as long as the composition for oral cavity of this invention is a range which can be used in an oral cavity, the pH will not be restrict | limited, However, Usually, pH is 3.0-10.5, Preferably it is pH 5.5-8.0.

  As a medicinal component, in addition to cetylpyridinium chloride as a bactericidal agent, for example, cationic bactericides such as chlorhexidine hydrochloride, chlorhexidine gluconate, benzethonium chloride, benzalkonium chloride; amphoteric bactericides such as dodecyldiaminoethylglycine; isopropylmethylphenol, Nonionic fungicides such as triclosan; Enzymes such as dextranase, amylase, protease, mutanase, lysozyme, lytic enzyme (lytechenzyme); Glycyrrhizinate such as glycyrrhetinic acid and dipotassium glycyrrhizinate as anti-inflammatory agents; Nicotinic acid or tocopherol acetate, etc .; Tranexamic acid, epsilon aminocaproic acid, etc. as antiplasmin agent; Ascorbic acid, etc. as bleeding remedy; Fluorine compounds such as sodium fluoride as remineralizing agents; plant extracts extracted with water-soluble solvents, chlorophyll, sodium chloride, caropeptides, zinc chloride, hinokitiol, etc. It can mix | blend combining a seed | species or more.

  Among the above components, the oral composition containing xylitol, maltitol, erythritol, and palatinit suppresses the astringency and bitterness of the methyl mercaptan generation inhibitory component added to the oral composition, and the oral composition is preferred. It is preferable for enhancing the properties. It is preferable to mix | blend 1-10 mass% with respect to the composition whole quantity, and it is more preferable to mix | blend 5-10 mass%. In addition, it is not preferable to add more than 10% by mass since the possibility of precipitation of crystals such as xylitol from the composition adhering to the discharge port of the container or the inside of the cap increases when the oral composition is used.

  Hereinafter, the present invention will be specifically described, but the present invention is not limited to the following examples. In the following, “%” means “mass%” unless otherwise specified.

Evaluation of Methyl Mercaptan Generation Suppression Effect The methyl mercaptan generation suppression effect was measured according to the following method.
After adding 770 μL of the salt buffer, 100 μL of the test solution, and 100 μL of the bacterial suspension to a glass bottle of 15 mL volume, and shaking lightly by hand, 30 μL of 33 mM L-methionine salt buffer was added, followed by incubation at 37 ° C. for 90 minutes. Immediately after culturing, 500 μL of 3M phosphoric acid aqueous solution was added and stirred, and allowed to stand for 10 minutes. Then, 1 mL of gas was sampled from the head space of the glass bottle, and injected into a gas chromatograph to quantify methyl mercaptan. The analysis conditions are the same as those described in the evaluation of the removal effect of methyl mercaptan. The methyl mercaptan generation inhibition rate (%) is defined as follows: Ki is the amount of methyl mercaptan when the test solution is added, and K0 is the amount of methyl mercaptan when the salt buffer is added instead of the test solution. Used to calculate. As a blank, 40 mM potassium phosphate buffer (pH 7.7) to which 50 mM sodium chloride was added was used. The obtained results are shown in Table 2.

Occurrence suppression rate (%) = [(K0−Ki) / K0] × 100

The bacteria, reagents, test solution, culture medium, and bacterial suspension used in the test were as follows.
Bacteria used: Porphyromonas gingivalis strain W83 (hereinafter sometimes abbreviated as Pg)
Salt buffer solution: 40 mM aqueous potassium dihydrogen phosphate solution was added to 40 mM aqueous dipotassium hydrogen phosphate solution to adjust the pH to 7.7. 1 L of the solution was taken and 2.92 g of sodium chloride was added and heat sterilized.
Test solution: Each test sample was prepared using a salt buffer so as to have a prescribed concentration in terms of solid matter.
Medium: First, 0.25 g of hemin was dissolved in 5 mL of 1N sodium hydroxide, and 20 mL of distilled water was further added to make solution A. A solution B was prepared by dissolving 0.025 g of menadione in 25 mL of 99% ethyl alcohol. The solutions A and B were mixed to prepare a hemin and menadione solution. The medium used was an autoclaved solution obtained by dissolving 40 g of TSB, 1 mL of hemin, menadione solution and 1 g of Yeast product in 0.9 L of distilled water. This medium is hereinafter referred to as “TSB + h, m, y”.
Bacterial suspension: Pg was inoculated into TSB + h, m, y 10 ml and anaerobically cultured at 37 ° C. for 24 to 48 hr. Anaerobic culture was performed. This bacterial culture was centrifuged at 8000 × g for 10 minutes, washed with a salt buffer, suspended in a salt buffer, and adjusted to about 0.3 at an OD550 nm of 33 mM L-methionine salt buffer solution: L- Methionine was prepared using the salt buffer solution so as to have a final concentration of 33 mM and sterilized by filtration.
3M phosphoric acid aqueous solution: A phosphoric acid and distilled water were used to prepare a final concentration of 3M.

The following samples were used.
Olive leaf extract: Opiace (powder: Eisai Food Chemical Co., Ltd.)
Novara fruit extract: Falco Rex Novara B (manufactured by Ichimaru Falcos)
Hawthorn Extract: Falco Rex Hawthorn B (manufactured by Ichimaru Falcos)

  As shown in Table 1, it was found that the olive leaf extract has an excellent methyl mercaptan generation inhibitory effect. In particular, it was found that when 0.001% by mass or more is blended, an inhibitory effect of 50% or more is obtained, and when 0.01% or more is blended, a suppressive effect of 75% or more is obtained. On the other hand, it was found that nocturnal fruit extract or hawthorn extract could not obtain any inhibitory effect despite the blending of 0.01% by mass or more.

Evaluation of Bitterness / Astringency Inhibitory Effect According to the following method, the bitterness / astringency-inhibiting effect of an oral composition containing a methyl mercaptan generation inhibitor was evaluated.
Examples 1 to 6 and Comparative Example 1 produced by a conventional method were subjected to absolute evaluation using five grades based on the following criteria using five specialist panels. For the evaluation, 10 ml of each subject was taken and contained in the mouth for about 20 seconds. The obtained evaluation points were totaled, the average value of the evaluation points was calculated for each evaluation item, and the results are shown in Table 3.
Evaluation point 5: Feel weak
4: Feel somewhat weak
3: Neither can be said
2: I feel a little stronger
1: Feel strong

  As shown in Table 2, when 1 to 10% by mass of xylitol, maltitol, and erythritol is added to the total amount of the oral composition, the bitterness and astringency of the oral composition containing the olive leaf extract are remarkably suppressed. I understood it.

  Hereinafter, although the prescription of the Example of the composition for oral cavity which mix | blended the methyl mercaptan generation | occurrence | production inhibitor based on this invention is given, this invention is not limited to the following prescription.

Formulation Example 1
Granule capsules were prepared according to a conventional method.
Granule capsules were prepared by encapsulating 60 parts by weight of the solution in the capsule with 40 parts by weight of the inner capsule film made of gelatin and sorbitol, and sugar coating with 110 parts by weight of the outer capsule film made of sugar.

Solution in capsule
Component amount
Olive leaf extract 10.0
Vitamin C 7.2
Vitamin E 2.4
Glycerin fatty acid ester 1.0
Safflower oil balance
Total 60

Inner capsule film
Component amount
Gelatin 36.0
Sorbitol balance
Total 40

Outer capsule coating
Component amount
Eggshell calcium 1.0
Gum arabic 0.6
Shellac 0.3
Gelatin 0.2
Carbana wax 0.1
Aspartame 0.1
Fragrance 0.4
Palatinit rest
Total 110

Claims (4)

  An oral composition for suppressing methyl mercaptan generation containing an olive leaf extract as an active ingredient.   It is a composition for oral cavity selected from the group which consists of a chewing gum agent, a candy agent, a troche agent, a tablet agent, a chewable tablet agent, and a drink, The methyl mercaptan generation | occurrence | production suppression of Claim 1 characterized by the above-mentioned. Oral composition.   The oral cavity for suppressing methyl mercaptan generation according to any one of claims 1 and 2, wherein the olive leaf extract is 0.001 to 10 mass% in terms of solid matter with respect to the total amount of the composition. Composition.   The composition for oral cavity for suppressing methyl mercaptan generation according to any one of claims 2 and 3, further comprising one or more selected from the group consisting of xylitol, maltitol, erythritol, and palatinit.