JP2010018544A - Antimicrobial agent, composition for oral cavity and denture cleanser - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an antimicrobial agent having excellent bactericidal and bacteriostatic activity against genus Candida fungus, those living in the oral cavity in particular, and also to provide a composition for the oral cavity and a denture cleanser each compounded with such an antimicrobial agent. <P>SOLUTION: The antimicrobial agent comprises a Sapindus rarak DC extract as active ingredient. Specifically, the antimicrobial agent comprises saponin extracted from Sapindus rarak DC. The composition for the oral cavity and the denture cleanser each containing the above antimicrobial agent are also provided. <P>COPYRIGHT: (C)2010,JPO&INPIT

Description

  The present invention relates to an antibacterial agent having an excellent bactericidal and bacteriostatic action, particularly against Candida fungi, and further relates to a composition containing the antibacterial agent, particularly an oral composition and a denture cleaning agent.

One of the typical superficial mycoses is candidiasis. Candidiasis is caused by infection with Candida fungi such as oral candidiasis, esophageal / intestinal candidiasis, and external vaginal candidiasis. In particular, oral candidiasis has increased remarkably recently with an increase in immunocompromised patients such as AIDS patients. In addition, it is preferable that the denture wearer removes the denture after meals and before going to bed and cleans the denture and removes the so-called denture plaque completely in addition to the food residue attached to the denture. However, when the denture user is not sufficiently cleaned or denture plaques are highly adherent, they may not be sufficiently removed, causing the onset of stomatitis. If you are affected by the onset of stomatitis, even if you are not a denture wearer, you will have severe pain just by putting food in your mouth. For those who have to struggle with mastication, such as a denture wearer, the pain is even greater, leading to loss of appetite and even worsening of the general condition.
Stomatitis has long been said to be caused by a lack of vitamin Bs, but recent dental and dermatological studies have shown that it can also be caused by fungi in the above-mentioned denture plaques, particularly Candida fungi. It was. Therefore, it is extremely important for the prevention of stomatitis to completely remove Candida bacteria in the denture plaque with, for example, a detergent.

Conventionally, an extract from a plant has attracted attention as an antibacterial component, and in hair cosmetics, a saponin obtained by extraction from the pericarp of Sapindus mukurossi G _{AERTN. Or} the pericarp of Sapindus delavayi R _ADLK. Is proposed as an antibacterial component (see Patent Document 1), and an anti-dermatophytic agent containing an extract from the skin of mukuroji, skin coconut or Sapindus trifoliatus is proposed. (See Patent Document 2). Furthermore, it is known that various plant-derived extracts, for example, extract of Sapindus rarak DC, are blended as an active ingredient of the hair restorer composition (see Patent Document 3).
On the other hand, in view of oral candidiasis, problems of oral hygiene of denture users as described above, and the cause of stomatitis, it is safe and excellent against Candida fungi, especially Candida fungi that are problematic in the oral cavity. There is a strong demand for antibacterial agents that exhibit bactericidal and bacteriostatic effects.

Japanese Patent No. 2588723 Japanese Patent No. 2727096 JP 2001-220320 A

  An object of this invention is to provide the antibacterial agent which has the outstanding bactericidal and bacteriostatic action with respect to Candida fungi, especially an intraoral Candida fungus. Another object of the present invention is to provide a composition for oral cavity and a denture cleaning agent containing such an antibacterial agent.

As a result of intensive studies to achieve the above-mentioned problems, the present inventors have demonstrated that an extract of a specific plant has an excellent bactericidal and bacteriostatic action against Candida fungi, especially Candida albicans. As a result, the present invention was completed.
Therefore, the present invention is an antibacterial agent comprising an extract of sapinda larack as an active ingredient. Among the extracts of sapinda larack, saponin is considered to be an active ingredient. Therefore, the present invention is also an antibacterial agent containing saponin extracted from sapinda larack.
The active ingredient of the antibacterial agent has extremely low toxicity and high safety, and the antibacterial agent is used in a wide range of fields such as oral compositions, denture cleaning agents, cosmetics, pharmaceuticals, foods, industrial products, and household products. Can be used.
Therefore, the present invention is further directed to an oral composition containing the antibacterial agent and a denture cleaning agent containing the antibacterial agent. More specifically, the present invention relates to an oral composition containing an extract of sapinda larack, a denture cleaning agent containing an extract of sapinda larack, an oral composition containing saponin extracted from sapinda larack, and sapinda larack. It is a denture cleaning agent containing saponin to be extracted.
Examples of the extract of sapinda larack include an extract of sapinda larra fruit, flesh, pericarp or a mixture thereof with water, a hydrophilic organic solvent or a hydrous hydrophilic organic solvent.

The active ingredient of the antibacterial agent of the present invention is highly safe and suitable for use as a component for oral compositions and denture cleaning agents, and its antibacterial and bacteriostatic action is Candida fungi, especially oral Candida. Excellent against the fungus Candida albicans.
The composition for oral cavity and denture cleansing agent containing the antibacterial agent is useful for keeping the user's oral cavity and denture hygienically good and prevents symptoms and inflammation caused by Candida fungi be able to.

The Sapindus rarak used in the present invention is a dicotyledonous plant belonging to the genus Mukuroji, which originates from Southeast Asia and is now widely grown in Asia and Africa. The fruit has a brown, soft skin and becomes dark brown when dried. The pericarp has the property of foaming in water and is traditionally used for washing as a natural soap. Fruits are available throughout the year and sold on the market. Sapindas is the meaning of Indian soap. Depending on the soapy nature of the peel. In India, it has been used for washing since ancient times.
In the present invention, the extraction raw material for obtaining an extract of sapinda larack is a dried sapinda larra fruit (including seeds, pulp and pericarp), flesh (which may include seeds), pericarp, or a mixture thereof. In general, it is used after pulverizing to a suitable size.

Examples of the extraction solvent include water, a hydrophilic organic solvent, a hydrous hydrophilic organic solvent or a mixture thereof, and specific examples thereof are selected from water, ethanol, 1,3-butylene glycol, propylene glycol, glycerin and diglycerin. There are one kind alone or two or more kinds in combination. Of these, ethanol and water-containing ethanol, for example, water-containing ethanol containing 70% by mass or more of ethanol are preferably used from the viewpoint of antibacterial action.
The ratio of the Sapinda larack extraction raw material and the extraction solvent at the time of extraction is not particularly limited, but it is common to use 2 to 1000 times the amount of solvent with respect to the extraction raw material based on the mass. In particular, the extraction solvent is preferably 5 to 100 times the amount of Sapinda larack extraction raw material in terms of extraction operation and efficiency.
The extraction conditions are not particularly limited, but the extraction temperature is from room temperature to the boiling point of the extraction solvent, for example, in the range of 20 to 35 ° C., preferably under normal pressure. As an extraction method, immersion extraction is suitable, and a stirring operation may be performed during immersion. Although the extraction time varies depending on the extraction temperature, it is generally in the range of 10 to 60 hours, for example, about 24 hours of immersion for about 24 hours. After the extraction operation, solid-liquid separation is performed by an appropriate method to obtain an extract. These extraction operations can be performed repeatedly with the raw material residue after the first extraction operation.

The extract of sapinda larac thus obtained can be used as it is, or can be concentrated by a known means, for example, concentrated under reduced pressure to obtain a concentrated extract. Alternatively, the extract may be subjected to operations such as deodorization and purification within a range not losing the antibacterial action to obtain a deodorized extract or purified product, or a fraction obtained using column chromatography or the like. Also good. Furthermore, a solid dried product may be obtained by evaporating the solvent from the extract, deodorized extract, purified product, and fraction. Furthermore, the solid dried product may be in the form of a liquid solubilized in a solvent such as alcohol, or in the form of an emulsion, or may be processed into a gel or granular form.
The extract of sapinda larlac, which is an active ingredient of the antibacterial agent of the present invention, includes the above extract, concentrated extract, deodorized extract, purified product, fractionated product, solid dried product, liquid, emulsion form, gel-like Any form such as granular may be used.

The antibacterial agent of the present invention can be used in a wide range of fields such as oral compositions, denture cleaning agents, cosmetics, pharmaceuticals, foods, industrial products, and household products. It is aimed at denture cleaning agents.
The antibacterial agent of the present invention may have a composition of the extract of the sapinda larack, which is an active ingredient, or may contain other components in addition to the active ingredient.

The dosage form of the composition for oral cavity containing the antibacterial agent of the present invention is not particularly limited as long as it acts in the oral cavity as an external preparation, and various ingredients are blended to prepare pharmaceuticals, quasi drugs and foods. It can provide as various forms. Specifically, toothpaste, liquid dentifrice, liquid dentifrice, toothpaste such as toothpaste, powder dentifrice, mouthwash, mouthwash, gel for oral cavity, pasta for oral cavity, spray for oral cavity , Troches, tablets, chewables, capsules, films, granules, powdered juices, strawberries, chewing gums, candy, gummy candy and the like.
In the composition for oral cavity of the present invention, the extract of sapinda larack, which is an active ingredient of the antibacterial agent, can be blended in the range of 0.01 to 50% by mass, preferably 0.01 to 40% by mass, and more. Preferably it is the range of 0.05-25 mass%.

  The composition for oral cavity of this invention can be manufactured with a normal manufacturing method. The oral composition of the present invention has other components such as an abrasive, a wetting agent, an organic solvent, a binder, a fragrance, an excipient, a sweetener, a pH, depending on the form of the intended oral composition. Conditioners, preservatives, emulsifiers, solubilizers, surfactants, binders, lubricants, oils, pigments and the like can be appropriately blended. These additives are usually used in pharmaceutical compositions, oral compositions or food formulation designs, and can be appropriately selected within a range not impairing the effects of the present invention, and can be blended in an appropriate blending amount. it can. The following are mentioned as an example of such an additive.

As an abrasive, for example, calcium carbonate, calcium phosphate, dicalcium phosphate, calcium pyrophosphate, insoluble calcium metaphosphate, anhydrous silicic acid, titanium oxide, amorphous silica, crystalline silica, aluminosilicate, aluminum oxide, aluminum hydroxide, resin Hydroxyapatite and the like can be blended alone or in combination of two or more.
As the wetting agent, for example, polyhydric alcohols such as glycerin, sorbitol, polyethylene glycol, propylene glycol, ethylene glycol, 1,3-butylene glycol, polypropylene glycol, xylitol, maltitol, lactitol, or a combination of two or more thereof. Can be blended.

As the organic solvent, alcohol is preferable, and examples thereof include ethanol, propyl alcohol, and isopropyl alcohol, and ethanol is particularly preferable. These alcohols can be blended alone or in combination of two or more.
Examples of the binder include cellulose derivatives such as carrageenan, carboxymethylcellulose and sodium carboxymethylcellulose, alkali metal alginates such as sodium alginate, gums such as xanthan gum, tragacanth gum and gum arabic, and synthetic viscosities such as polyvinyl alcohol and sodium polyacrylate. Examples of the binder include inorganic binders such as silica gel, aluminum silica gel, and bee gum.

Examples of flavoring agents include anethole, menthol, peppermint oil, spearmint oil, lemon oil, orange oil, sage oil, rosemary oil, cinnamon oil, perilla oil, winter green oil, clove oil, eucalyptus oil, pimento oil, carvone. Synamic aldehyde, cineole, menthone, limonene, methyl salicylate and the like can be blended alone or in combination of two or more thereof within a range not impairing the effects of the present invention. The blending amount is generally 0.0001 to 1.0 mass% with respect to the total amount of the oral composition.
Examples of the sweetener include palatinit, aspartame, saccharin sodium, acesulfame potassium, stevioside, neohesperidyl dihydrochalcone, glycyrrhizin, perillamine, thaumatin, asparatylphenylalanyl methyl ester, ρ-methoxycinnamic aldehyde and the like. These can be blended singly or in combination of two or more. The compounding quantity is 0.01-1 mass% normally with respect to the whole composition for oral cavity, Preferably it is 0.05-0.5 mass%.

Examples of the pH adjuster include citric acid, phosphoric acid, pantothenic acid, malic acid, pyrophosphoric acid, lactic acid, tartaric acid, glycerophosphoric acid, acetic acid, nitric acid, or a chemically possible salt or sodium hydroxide thereof. These can be blended alone or in combination of two or more so that the pH of the composition falls within an appropriate range. The compounding quantity is 0.01-2 mass% normally with respect to the whole composition for oral cavity.
Examples of excipients include sucrose, lactose, starch, glucose, crystalline cellulose, mannitol, sorbitol, xylitol, erythritol, palatinit, palatinose, maltitol, trehalose, lactitol, lactulose, reduced starch sugar, reduced isomaltoligosaccharide. , Coupling sugar, gum base, gum arabic, gelatin, cetylmethylcellulose, light anhydrous silicic acid, magnesium aluminate, calcium aluminate metasilicate, sodium bicarbonate, calcium phosphate and the like.

Examples of preservatives include p-hydroxybenzoates, benzoic acid and salts thereof, salicylic acid and salts thereof, sorbic acid and salts thereof, phenoxyethanol, and alkyldiaminoethylglycine hydrochloride.
Examples of the foaming agent include sodium lauryl sulfate, sodium lauroyl sarcosine, sodium alkylsulfosuccinate, sodium coconut oil fatty acid monoglycerol sulfonate, sodium α-olefin sulfonate, N-acyl glutamate and the like, 2-alkyl- N-carboxymethyl-N-hydroxyethylimidazolinium betaine, maltitol fatty acid ester, sucrose fatty acid ester, polyglycerin fatty acid ester, fatty acid diethanolamide, polyoxyethylene sorbitan monostearate, polyoxyethylene hydrogenated castor oil, polyoxy Ethylene fatty acid ester etc. are mentioned, Among these, 1 type (s) or 2 or more types can be used together.

Examples of the solubilizer include esters, polyethylene glycol derivatives, polyoxyethylene hydrogenated castor oil, sorbitan fatty acid esters, sulfated fatty alcohols and the like.
Examples of the lubricant include magnesium stearate, sucrose fatty acid ester, talc, and hardened oil.
Examples of the oil include coconut oil, olive oil, sesame oil, peanut oil, parsley oil, parsley seed oil, safflower oil, and the like.

Furthermore, the composition for oral cavity of the present invention includes vitamin E such as dl-α-tocopherol acetate, tocopherol succinate or tocopherol nicotinate, vitamin C such as ascorbic acid, dextranase, amylase, protease, mutanase. , Lysozyme, enzymes such as lytic enzyme (lytechenzyme), anti-plasmin agent of tranexamic acid and epsilon aminocaproic acid, aluminum chlorohydroxyl allantoin, dihydrocholesterol, glycyrrhizin salts, glycyrrhizic acid, glycerophosphate, chlorophyll, sodium chloride, caropeptide, water-soluble An inorganic inorganic phosphate compound, cetylpyridinium chloride, triclosan, hinokitiol, chlorhexidine hydrochloride, etc., alone or in combination of two or more. It can be.
The composition for oral cavity of this invention can mix these components and can manufacture it according to a normal method.

The denture cleaning agent containing the antibacterial agent of the present invention can have various shapes, for example, liquid (emulsified form, solubilized form), liquid form, gel form, paste form, tablet, effervescent tablet, powder form, It can be granular. Preparation into these dosage forms can be performed according to a conventional method.
In the denture cleaning agent of the present invention, an extract of sapinda larack, which is an active ingredient of the antibacterial agent, can be blended in the range of 0.01 to 50% by mass, preferably 0.01 to 40% by mass, more preferably Is in the range of 0.05 to 25% by mass.

In addition to the extract of sapinda larack, the denture cleaning agent of the present invention can further contain additives depending on the intended dosage form, such as solvents, preservatives, fragrances, and dentures. A cleaning component or the like usually used for a cleaning agent can be used. For example, if the shape is liquid, it can be produced by mixing with components such as wetting agents, surfactants, preservatives and the like. These additives can be used with the compounding quantity normally used.
Specific examples of the additive include the following components. Sodium lauryl sulfate, sodium N-lauroyl sarcosine, sodium cocoyl sarcosine, palm oil fatty acid amidopropyl betaine solution, 2-alkyl-N-carboxymethyl-N-hydroxyethylimidazolinium betaine, alkyldiaminoethylglycine hydrochloride, polyoxyethylene Surfactant such as hydrogenated castor oil, polyglycerin fatty acid ester; humectant such as glycerin, sorbitol, propylene glycol, 1,3-butylene glycol, polyethylene glycol; sodium carboxymethylcellulose, sodium alginate, carboxyvinyl polymer, xanthan gum, polyvinylpyrrolidone Thickeners such as carrageenan; solvents such as water and ethanol; sodium peroxide, sodium percarbonate, sodium perborate, Carbonates such as sodium oxyhydrogen and potassium carbonate; acid agents such as anhydrous citric acid, succinic acid, malic acid and tartaric acid; preservatives such as paraoxybenzoic acid ester and sodium benzoate; fragrances, pigments, isopropylmethylphenol, sodium chloride , chlorhexidine compound, click Rolle chlorhexidine sodium gluconate, benzalkonium chloride, edetate, etc. tripolyphosphate.

  The method of using the denture cleaning agent of the present invention can be used for dentures as it is or depending on the dosage form, or dissolved in an appropriate amount of water at the time of use. For example, about 2 to 3 g of tablets or granules are dissolved in about 200 g of water and used. Moreover, the denture cleaning agent of this invention can be used similarly to a normal denture cleaning agent.

Further, the present invention will be described in detail by examples and experimental examples.
[Example 1]
Extract of sapinda larack fruit An extract was obtained from sapinda larra fruit (including seeds, pulp and skin) by the following procedure.
10 g of sapinda larrac seeds dried and ground and 100 mL of water-containing ethanol (ethanol concentration 70% by mass) were mixed and allowed to stand at room temperature (25 ° C.) for 24 hours. The sapinda lalacquer seed was removed by filtration through a filter to obtain a sapinda lalacquer seed extract.

[Experimental Example 1]
MIC measurement for Candida albicans GP culture medium (manufactured by Wako Pure Chemical Industries, Ltd.) containing the sapinda larrac seed extract of Example 1 at the concentrations shown in Table 1 was inoculated with Candida albicans (ATCC 10231) by the multiple dilution method. After culturing at 5 ° C. for 2 days, the minimum growth inhibitory concentration (MIC) of the antibacterial agent was determined from visual confirmation of the growth state. In addition, instead of sapinda larrac fruit extract, as a sample for comparison, yucca 1% solution (powdered yucca extract (product of Mitsuba Trading, trade name: Yucca Sarasaponin 50-M) aqueous solution), and water content The same test was performed using ethanol (ethanol concentration 70% by mass).
Table 1: MIC for C. albicans of sapinda larrac seed extract
(-: Non-growth +: growth)

[Experiment 2]
Measurement of MIC and MBC for various bacteria The MIC and minimum bactericidal concentration (MBC) for various bacteria of the sapinda larac fruit extract obtained in Example 1 were determined by the multiple dilution method using the following medium.
Candida albicans (ATCC 10231) <using GP medium>, Escherichia coli (ATCC 8739), Pseudomonas aeruginosa (ATCC 9027), Staphyrococcus aureus (ATCC 6538P) <using BHI medium>, Streptococcus mutans (ATCC 25175) <SCD Medium use>, Propionibacterium acnes (ATCC 6919), Porphyromonas gingivalis (ATCC 33277) <GAM medium use> were used.
The results are shown in Table 2.

表２の結果より、サピンダス ララク抽出物は、Candida albicansに対して低濃度で抵抗性を示し、これは他種菌への抗菌力と比べて顕著であり、抗菌力に選択性があることがわかった。 Table 2: MIC / MBC for various fungi of Sapinda larrac seed extract

From the results in Table 2, it can be seen that Sapinda larrac extract is resistant to Candida albicans at a low concentration, which is remarkable in comparison with antibacterial activity against other species and has selectivity in antibacterial activity. It was.

[Experiment 3]
Confirmation of inhibition circle formation against Candida albicans 0.1 mL of Candida albicans (ATCC 10231) was inoculated on a plate agar medium made with potato dextrose granule agar (manufactured by Nissui Pharmaceutical), smeared, and for paper disc antibiotic test (ADVANTEC) The product was immersed in the sapinda larrac seed extract of Example 1 and cultured at 32.5 ° C. for 2 days, and formation of inhibition circles was confirmed. In addition, instead of Sapinda larrac fruit extract, as a sample for comparison, yucca 1% solution (powdered yucca extract (product of Mitsuba trade, trade name: Yucca Sarasaponin 50-M) aqueous solution), and water content The same test was performed using ethanol (ethanol concentration 70% by mass).
The diameter of the blocking circle for each sample was as follows:
Table 3

[Experimental Example 4]
Bactericidal activity test against andida albicans planktonic fungus The sapinda larac fruit extract obtained in Example 1 was tested for bactericidal activity against Candida albicans (ATCC 10231) planktonic fungus.
20 mL of a 3.13% solution of Sapinda larrac seed extract was prepared and inoculated with 20 μL of 1.0 × 10 ⁷ cfu / mL of Candida albicans (ATCC 10231) suspension using physiological saline. After incubating at 25 ° C. for 8 hours, the number of viable cells after 1, 2, 4, and 8 hours was measured by a pour culture method using a potato dextrose granule agar medium. In addition, it tested similarly using the 2.19% solution of ethanol as a sample for a comparison.
The results are shown in Table 4.

表４から明らかなように、サピンダス ララク抽出物との接触により、Candida albicans生菌数は経時的に減少し、８時間後には３０cfu/mL以下となった。 Table 4: Time course of Candida albicans viable count (unit: cfu / mL)

As is clear from Table 4, the viable count of Candida albicans decreased with time by contact with the Sapinda larrac extract, and after 8 hours became 30 cfu / mL or less.

[Experimental Example 5]
Denture plaque cleaning test Using the sapinda larrac seed extract obtained in Example 1, the following test was conducted.
A polymerized resin was molded on the bottom of a 24-well microplate, and 50 μL of Candida albicans (ATCC 10231) suspension 1.0 × 10 ⁷ cfu / mL using physiological saline was inoculated. After incubating at 32.5 ° C. for 2 hours, the bacteria were allowed to adhere to the resin plate. To this, 2 mL of BHI medium was added and incubated at 32.5 ° C. for 4 days to prepare a Denture plaque model.
After removing the obtained Denture plaque model medium and washing with sterilized purified water, a 3.13% solution of sapinda larrac real extract was contacted and removed at 25 ° C. for 8 hours, washed with sterilized purified water, The amount of ATP was measured by the chemiluminescence method. Denture plaque detergency with Sapinda larrac extract was expressed as a percentage (%) according to the following formula. In addition, it tested similarly using the 2.19% solution of ethanol as a sample for a comparison.
Detergency (%) = 100 − {(ATP amount after 8 hours contact with Sapinda larrac extract / ATP amount before contact with Sapinda larra extract) × 100}
The results are shown in Table 5.

表５の結果より、サピンダス ララク抽出物が優れた菌体洗浄力を有し、８時間で99.33%のDenture plaqueを洗浄したことがわかる。 Table 5: Denture plaque cleaning power (%)

From the results in Table 5, it can be seen that the Sapinda larrac extract has excellent microbial cell detergency and washed 99.33% Denture plaque in 8 hours.

[Experimental Example 6]
Bactericidal power test against denture-adhering bacteria The bactericidal power test of the sapinda larrac seed extract obtained in Example 1 against denture-adhering bacteria was performed.
Dentures were immersed in 200 mL (1.0 × 10 ⁷ cfu / mL) suspension of Candida albicans (ATCC 10231) using physiological saline at 25 ° C. for 1 hour. The extracted denture was immersed in 100 mL of a 3.1% solution of Sapinda larrac real extract at 25 ° C. for 8 hours. The number of viable bacteria adhering to the extracted denture was measured by a wiping method. The culture was performed at 32.5 ° C. for 2 days. In addition, it tested similarly using the 2.19% solution of ethanol as a sample for a comparison.
For the agar medium in which the sample from the denture after 1 hour immersion in Candida albicans suspension and the sample from the denture after 8 hours immersion in 2.19% ethanol solution were cultured by the wiping method, 1.0 × A viable cell count of 10 ³ cfu / mL was observed. On the other hand, a viable cell count of <30 cfu / mL was observed in the agar medium in which the sample from the denture after 8 hours immersion in the Sapinda larrac extract solution was cultured. From this result, it can be understood that the denture-adherent bacteria were sterilized by the Sapinda larack extract.

The formulation example of the oral cavity composition of this invention and denture cleaning agent which mix | blended the sapinda larac fruit extract obtained in the said Example 1 with the following and prepared by the conventional method is shown. The unit of the amount is mass%.
[Example 2]
Dentifrice (Toothpaste)
Ingredients Blending amount Sapindas Rarak fruit extract 0.7
Silicic anhydride 20.0
Glycerin 28.0
Titanium dioxide 0.3
Sodium carboxymethylcellulose 1.3
Methyl paraoxybenzoate 0.2
Saccharin sodium 0.1
Xylitol 9.0
Dl-tocopherol acetate 0.05
Peppermint flavor 0.6
Purified water balance 100.0

[Example 3]
Mouthwash component Formulation amount Sapindus Larack fruit extract 1.6
Ethanol 10.0
Tranexamic acid 0.05
Glycerin 5.0
Citric acid 0.01
Sodium citrate 0.1
Polyoxyethylene hydrogenated castor oil 0.5
Methyl paraoxybenzoate 0.1
Mint perfume Appropriate amount of purified water The remaining balance 100.0

[Example 4]
Oral gel component Formulation amount Sapindas Larack fruit extract 1.3
Carboxymethylcellulose 0.2
Glycerin 40.0
Purified water balance 100.0

[Example 5]
Tablet ingredient amount (%)
Sapinda rarak extract 20.0
Maltitol 42.0
Lactose 20.0
Lactulose 15.0
Sucrose fatty acid ester 3.0
Total 100.0

[Example 6]
Candy Ingredients Blending amount Sapindus Larack seed extract 6.2
Vitamin C 5.0
Xylitol 8.0
Maltitol 10.0
Aspartame 0.1
Fragrance 0.2
Palatinit remaining balance 100.0

[Example 7]
Chewable ingredients Formulation amount Sapindas Larack fruit extract 46.2
Maltitol 30.0
Xylitol 20.0
Sucrose fatty acid ester 3.0
Fragrance 0.8
Total 100.0

[Example 8]
Chewing gum ingredients Blending amount Sapindus Rarak fruit extract 13.5
Calcium carbonate 5.0
Vitamin E 0.1
Gum base 27.0
Erythritol 10.0
Xylitol remaining maltitol 12.0
Fragrance 0.5
Total 100.0

[Example 9]
Lozenge ingredients Blending amount Sapindas Rarak seed extract 16.6
Maltitol 21.0
Gum arabic 1.5
Sucrose fatty acid ester 2.5
Powder flavor 1.0
Citric acid 4.0
Vitamin C 10.0
Xylitol remaining balance 100.0

[Example 10]
Denture cleaning agent (liquid)
Ingredients Blending amount Sapindas Larack extract 2.0
Sodium lauroyl sarcosinate 20.0
Chlorhexidine sodium gluconate 2.0
EDTA sodium 6.0
Ethanol 60.0
Purified water 10.0
Perfume remaining total 100.0
The above ingredients were mixed well to form a liquid. The 0.5-1.5 g is dissolved in 100-150 mL of water and used.

[Example 11]
Denture cleaning agent (powder)
Ingredients Blending amount Sapindas Larack extract 23.0
Sodium bicarbonate 31.0
Citric acid 25.0
Sodium lauryl sulfate 6.0
Sodium tripolyphosphate 13.0
Polyethylene glycol 2.0
Perfume remaining total 100.0
The above ingredients were mixed well to obtain a powder. The 1.0-2.5g is melt | dissolved and used for 100-150mL water.

[Example 12]
Denture cleaning agent (tablet)
Ingredients Blending amount Sapindas Larack extract 10.0
Sodium bicarbonate 30.0
Tartaric acid 27.0
Sodium cocoyl sarcosinate 5.0
Sodium lauryl sulfate 5.0
Benzalkonium chloride 2.0
EDTA sodium 5.0
Sodium tripolyphosphate 14.0
Polyethylene glycol 2.0
Perfume remaining total 100.0
The above ingredients were mixed well, and 2-3 g thereof was tableted with a tableting machine. One tablet is dissolved in 100 to 150 mL of water and used.

Claims (4)

  An antibacterial agent containing an extract of sapinda larack as an active ingredient.   An antibacterial agent containing saponin extracted from sapinda larack.   The composition for oral cavity containing the antibacterial agent of Claim 1 or 2.   A denture cleaning agent comprising the antibacterial agent according to claim 1 or 2.