JP2013048564A - Method for extracting placenta extract and nutrient supplement - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a method for extracting a placenta extract suppressed in Maillard reaction generated during an extraction treatment process of the placenta extract and improved in each of properties such as a taste, color tone, and smell, and to provide a nutrient supplement containing the placenta extract obtained by the extraction method.SOLUTION: In a treatment process of separating and extracting an active ingredient of the placenta derived from a mammal, organic germanium fine powder is dissolved in a treatment liquid including the placenta, and the Maillard reaction is suppressed by performing the extraction treatment in the presence of the organic germanium.

Description

  The present invention relates to a placenta extract extraction method for separating and extracting an active ingredient from a placenta derived from a mammal, and a nutritional supplement containing the placenta extract obtained by the extraction method.

  Placenta derived from pigs, horses, sheep, etc. belonging to the placenta among mammals are condensed with physiologically active substances essential for species maintenance and survival, medical injections, over-the-counter drugs, medicinal cosmetics It is widely used as a general cosmetics and supplement for nutrition.

  For use in the above-mentioned fields, placenta is not used as it is, but placenta extract obtained by extracting useful components contained in placenta, that is, proteins, peptides, sugars, microRNAs, and the like is often used.

  As extraction methods for useful placenta components, various methods such as an enzymatic decomposition method, a hydrochloric acid decomposition method, an alkali extraction method, a freeze-thaw method, a purified water extraction method, and a hot water extraction method have been considered. The following Patent Document 1 discloses a technique for promoting enzymatic digestion at a temperature of 25 to 65 ° C. using a heat-resistant neutral proteolytic enzyme selected from protease S, papain W-40, protease N or protease P. Yes.

JP 2004-97033 A

  In the method for producing placenta extract disclosed in Patent Document 1, normal formulation, that is, placenta is refrigerated at 2-4 ° C. for about 4 days, washed, finely crushed, added with acetone, homogenized, degreased and dried, The dried product is acidified (pH 2.0) by adding hydrochloric acid, digested with pepsin all day and night, centrifuged to collect the digested solution, and the precipitate is heated with hydrochloric acid (5N) and hydrolyzed. The hydrolysis solution is decolorized with activated carbon, filtered, mixed with pepsin digestion solution, hydrochloric acid is removed with an anion exchange resin, pH is adjusted to 6.1 to 6.4 using caustic soda, and a placenta extract is obtained. In the case of this method, the treatment such as pH adjustment and removal of hydrochloric acid after completion of digestion becomes complicated, so that the special heat-resistant neutral proteolytic enzyme described above is used in order to eliminate the complexity. Is.

  As described above, general extraction of placenta extract is often performed by placing placenta in an extraction solvent mainly composed of purified water and heating it using an enzyme, an acid, or the like. The useful components to be extracted are biological components such as proteins, peptides, amino acids, nucleic acids, and sugars.

  By the way, it is known that Maillard reaction occurs when reducing sugars and amino compounds (proteins, peptides, amino acids) among these biological components are heated by heating. When this Maillard reaction occurs, an Amadori compound is produced.

  The Amadori compound is not preferable in various properties such as taste, color tone and odor, and reduces the economic value as a food or supplement. In the case of the placenta extract production method according to Patent Document 1, it goes without saying that the Maillard reaction occurs and a brown substance (melanoidin) is produced.

  As for the Maillard reaction in the extraction process of the placenta extract, no accurate suppression method has been proposed so far, and therefore, an unfavorable placenta extract has been used.

  The object of the present invention is to contain a placenta extract extraction method that suppresses the Maillard reaction that occurs during the extraction process of the placenta extract and improves each property such as taste, color tone, and odor, and the placenta extract obtained by the extraction method. It is to provide a nutritional supplement.

  If the structure of this invention for solving the said subject is explained in full detail, the invention which concerns on Claim 1 will be organic in the process liquid containing a placenta in the process process which isolate | separates and extracts the active ingredient from the placenta derived from a mammal. A placenta extract extraction method characterized by dissolving germanium fine powder and performing an extraction treatment in the presence of the organic germanium to suppress the Maillard reaction.

  The invention according to claim 2 is the placenta extract extraction method according to claim 1, wherein the organic germanium is carboxygermanium sesoxide (Ge-132).

  In the invention according to claim 3, the dissolution concentration of the organogermanium in the processing solution containing the placenta is in the range of 0.01 ppm to 100 ppm, preferably in the range of 0.1 ppm to 10 ppm. The placenta extract extraction method according to any one of 2 above.

  The invention according to claim 4 is characterized in that the treatment step includes one or more extraction treatment methods selected from enzymatic decomposition, hydrochloric acid decomposition, alkali extraction, freeze-thawing, purified water extraction, and hot water extraction. It is the extraction method of the placenta extract in any one of Claim 1 thru | or 3.

  The invention according to claim 5 is a nutritional supplement containing the placenta extract obtained by the method for extracting placenta extract according to any one of claims 1 to 4.

  According to the method for extracting placenta extract according to the present invention, organogermanium dissolved at a constant concentration in a treatment solution containing placenta acts as a suppressor of the Maillard reaction, and taste and color tone compared to placenta extract obtained by a conventional method. -A placenta extract with improved properties such as odor can be provided.

It is a graph which shows the result of having measured the light absorbency of visible light using the spectrophotometer for the placenta extract (specimen) obtained by the extraction method which concerns on this invention. It is a graph which shows the result of having measured the light absorbency of visible light using the spectrophotometer for the placenta extract (control) obtained by the conventional extraction method.

  Hereinafter, a specific configuration of a placenta extract extraction method and a nutritional supplement supplement containing the placenta extract obtained by the extraction method according to the present invention will be described in detail.

  As described above, there are a plurality of methods for extracting useful components in placenta derived from mammals. However, in any of the extraction methods, pH fluctuations, heating, and the like occur during the treatment process, and a reducing sugar and an amino compound (protein, peptide, amino acid) react to produce a brown substance. This Maillard reaction is a reaction that starts with a bond between a sugar and an amino group and then reaches a Amadori product via a Schiff base. Amadori products are not preferred for the properties of placenta extract.

  The present invention provides a placenta extract having good properties by suppressing the Maillard reaction that occurs during the extraction process of placenta extract. In the process of separating and extracting the active ingredient from placenta derived from mammals, a process comprising placenta The content is to dissolve the organic germanium fine powder in the liquid and perform the extraction treatment in the presence of the organic germanium.

  A plurality of organic germaniums such as trialkylgermyl acetate, carboxygermanium sesoxide, and dimethyl (porphyrinato) germanium are known. Among them, carboxygermanium sesoxide (Ge-132) having excellent safety is disclosed in the present invention. Most preferred for implementation. The Maillard reaction is a reaction that naturally occurs if an amino group and a sugar are present, and the addition of organogermanium is preferably in the initial stage of extraction.

  If the dissolution concentration of the organic germanium in the treatment liquid containing placenta is in the range of 0.01 ppm to 100 ppm, the effect of suppressing the Maillard reaction can be obtained, but more preferably in the range of 0.1 ppm to 10 ppm. is there.

  The reason why the lower limit concentration in the treatment liquid was set to 0.01 ppm was that it was found that an effect of suppressing the Maillard reaction was hardly obtained at a concentration lower than this, and the upper limit concentration was set to 100 ppm. This is because it has been found that when the concentration is high, the enzymatic decomposition of the treatment liquid containing the placenta is inhibited and the extraction efficiency is lowered. The most preferable concentration range is 0.1 ppm to 10 ppm, and it was confirmed as a result of various experiments that the Maillard reaction can be satisfactorily suppressed.

The frozen placenta of the pig was naturally thawed, and unnecessary parts such as kuroko were removed.
On the other hand, a 0.1% aqueous solution of carboxygermanium sesoxide (Ge-132) was prepared using purified water. The 0.1% aqueous solution was further diluted 5000 times with purified water to prepare a 0.2 ppm aqueous solution of carboxygermanium sesoxide (Ge-132).

  Next, the 0.2 ppm aqueous solution was weighed so as to have the same amount as the minced placenta, and both were equally mixed together (corresponding to 0.1 ppm as Ge-132).

  Thereafter, the pH was adjusted to 7.0 using a buffer buffer, and enzymatic degradation was performed at about the optimum temperature (48 ° C.) for 10 hours while stirring using a neutral protease.

After the enzymatic decomposition, the precipitated components were removed with a centrifuge, and aseptic filtration was performed with a 0.2 μm membrane filter to obtain a placenta extract (specimen) according to the present invention.
As a control, placenta extract obtained by performing enzymatic degradation under the same conditions as described above using ordinary purified water that does not dissolve organic germanium fine powder was used.

  The placenta extract (specimen) obtained by the method of the present invention and the placenta extract (control) obtained by a conventional method were measured for the absorbance of visible light (380 nm to 750 nm) using a spectrophotometer. As a result, in the case of the placenta extract (specimen) obtained by the method of the present invention as shown in FIGS. 1 and 2, yellow (580 nm) is 10%, blue ( (470 nm), a 20% decrease in absorbance was confirmed.

  Evaluation of the color using a spectrophotometer revealed that the placenta extract extracted in the presence of organic germanium water was significantly lighter in color and suppressed the Maillard reaction.

  Next, a sensory test was conducted on the odor of the placenta extract (specimen) obtained by the method of the present invention and the placenta extract (control) obtained by a conventional method. The method was double-blind with a panel of 12 people.

As a result, 11 out of 12 respondents said that the placenta extract (specimen) obtained by the method of the present invention had a better odor than the placenta extract (control) obtained by the conventional method. The remaining 1 person was unknown.
The sensory test of the odor revealed that the placenta extract obtained by the method of the present invention has significantly less odor and the Maillard reaction is suppressed.

  Furthermore, a sensory test was conducted on the taste of the placenta extract (specimen) obtained by the method of the present invention and the placenta extract (control) obtained by a conventional method. The method was double-blind with a panel of 12 people.

When the panel of 12 people asked whether the sample or the control had a better taste, all 12 people selected the placenta extract obtained by the method of the present invention.
This sensory test on taste revealed that the placenta extract extracted by adding organic germanium water had significantly better taste and suppressed Maillard reaction.

  The placenta extract obtained by the method of the present invention can be sprayed by, for example, a spray drying method, rapidly dried to form a powder, filled in a soft capsule, and provided as a nutritional supplement or a cosmetic supplement. it can.

Claims (5)

  In the process of separating and extracting active ingredients from placenta derived from mammals, organic germanium fine powder is dissolved in a treatment solution containing placenta, and extraction treatment is performed in the presence of the organic germanium to suppress the Maillard reaction. A placenta extract extraction method characterized by the above.   The method for extracting placenta extract according to claim 1, wherein the organic germanium is carboxygermanium sesoxide (Ge-132).   The placenta according to claim 1 or 2, wherein a dissolution concentration of the organic germanium in the treatment liquid containing the placenta is in a range of 0.01 ppm to 100 ppm, preferably in a range of 0.1 ppm to 10 ppm. Extract extraction method.   4. The process according to any one of claims 1 to 3, wherein the treatment step includes one or more extraction treatment methods selected from enzymatic decomposition, hydrochloric acid decomposition, alkali extraction, freeze-thawing, purified water extraction, and hot water extraction. Extraction method of placenta extract.   A nutritional supplement comprising the placenta extract obtained by the method for extracting a placenta extract according to any one of claims 1 to 4.

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