JP2012228276A - Apparatus for ozone sterilization of co2 incubator - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an apparatus that ensures sterilization of a COincubator, has no leakage of fed ozone gas to the outside, uniformly circulates ozone in a storage and constantly and continuously controls an ozone concentration.SOLUTION: The apparatus for ozone sterilization of a COincubator includes: a sterilization tent 1 with an airtight hole 8a for airtightly protruding a leading end part of a tube connection part 14a provided in the COincubator 11 to the outside and a discharge part 9a for discharging ozone gas to the outside, for covering the COincubator 11 airtightly; an ozone gas generator 18 for applying circulating sterilization to the inside and the outside of the COincubator 11 covered by the sterilization tent 1 by pressure-feeding the ozone gas to the tube connection part 14a; and an ozone gas neutralization unit 26 for neutralizing and eliminating the ozone gas discharged from the discharge part 9a.

Description

The present invention relates to a sterilizer for CO ₂ incubator used for storage and cultivation of various cells, as well as to allow complete sterilization of CO ₂ incubator, the CO ₂ incubator in the storage material during sterilization safety, and the sterilization gas The purpose is to prevent danger from leakage.

  As a means for sterilizing surgical medical instruments and pharmaceutical containers in the conventional medical field, wipe with alcohol before or after the use of surgical medical instruments and pharmaceutical containers, or sterilize by high-temperature sterilization at about 140 ° C, or sterilize by UV irradiation. It has been known. It is also known to use ethylene oxide such as toxic ethylene oxide gas in which propane gas is mixed or formalin gas in a sterilization chamber for sterilizing surgical medical instruments and pharmaceutical containers.

  However, when using toxic ethylene oxide gas or formalin gas mixed with propane gas in the sterilization chamber, a separate cleaning device is required to remove the residual gas after sterilization, and it is harmful even if the cleaning device is used. Since there is a problem that it is difficult to completely remove the residual gas, recently, ozone gas with the property of automatically disappearing with time is used to depressurize the inside of the pressure vessel containing the object to be sterilized. The object to be sterilized is sterilized by jetting ozone gas circulated in the pressure vessel into the pressure vessel, and removed outside the pressure vessel after sterilization (Japanese Patent Laid-Open No. 9-271512) Reference) was developed.

  Also, by connecting an ozone line and an aeration line with a sterilization filter in a sterilization apparatus containing a sterilization target such as a medical instrument, and connecting a bypass line between the sterilization filter upstream side of the aeration line and the ozone line Also known is one in which ozone gas is allowed to flow directly to the sterilization filter through a bypass line for sterilization (see JP 2001-340432 A).

Furthermore, in order to sterilize the inside of an incubator for culturing microorganisms such as various cells and fungi, a CO ₂ cylinder is connected to the chamber via a three-way switching valve 11, and the chamber is placed at a required position. Connect a circulation line with a gas circulation pump, CO ₂ sensor, O ₂ sensor, and ozone sensor in the middle, and connect an exhaust line equipped with an ozone decomposition device to the three-way switching valve 16 in the middle of the circulation line. Thus, at the end of the culture, the three-way switching valve 11 is switched so that the ozone generator communicates with the inside of the chamber, and ozone gas is introduced and filled in the chamber to sterilize the inner surface of the chamber and the internal atmosphere so that the next culture is performed. (See JP 2004-267064 A).

  Furthermore, it is also known that a clean room is configured in units of rooms and the entire room is filled with ozone gas and sterilized.

JP-A-9-271512 JP 2001-340432 A JP 2004-267064 A

However, in the case of a CO ₂ incubator for culturing microorganisms such as various cells and fungi, it is necessary to perform complete sterilization in the CO ₂ incubator at the end of the culture. In particular, when culturing cells, it is necessary to check the sterility of the CO ₂ incubator before culturing. Generally, for example, an agar medium is placed in the chamber and allowed to stand for a certain period of time. It needs work to confirm.

In this case, a sterilization means by ozone gas circulation as described in Patent Document 3 described above, that is, a CO ₂ cylinder is connected to the outside of the CO ₂ incubator via a three-way switching valve. ₂ sensors, O ₂ sensor, connect the circulation line is interposed ozone sensor, moreover by connecting an exhaust line equipped ozonolysis apparatus three-way valve 16 in the middle of the circulation line, three-way valve to the end of the culture 11 is switched so that the ozone generator communicates with the inside of the chamber, and ozone gas is introduced and filled in the chamber to sterilize the inner surface of the chamber and the internal atmosphere, and then the next culture is performed. Problems are likely to occur.

In other words, the CO ₂ incubator currently in use is not completely airtight, so it is dangerous because ozone gas leaks from the culture chamber to the outside of the CO ₂ incubator even though it has the property of automatically disappearing over time. , also sometimes ozone does not span to go evenly to every corner of a CO ₂ incubator, the function of the sterilization of a CO ₂ incubator is not necessarily enough. In addition, when using a CO ₂ incubator, it is necessary to carefully wipe the outer surface of the incubator beforehand with a piece of cloth or non-woven fabric soaked with alcohol or ethanol, etc. Be careful when using a CO ₂ incubator. To complete sterilization across the inside and outside of the incubator.

  In addition, a clean room is configured in units of rooms, and in order to fill and sterilize ozone gas throughout the room, not only a large amount of ozone gas is required, but also the danger to workers, especially the presence or absence of workers in the clean room However, this is not a very effective means, because there is a risk of gas leakage outside the room, and there is a risk of oxidation of various devices installed in the clean room.

Therefore, in the present invention, the CO ₂ incubator can be completely sterilized, in particular, the ozone sent into the CO ₂ incubator is not leaked to the outside, and the ozone can be circulated evenly in the warehouse. In addition to taking out ozone gas after ozone sterilization work and neutralizing and eliminating it, there is also a means to fully check for the presence of residual bacteria in the CO ₂ incubator after sterilization work. Is.

Specifically, an ozone sterilization apparatus for a CO ₂ incubator according to a first aspect of the present invention is an ozone sterilization apparatus for circulating and sterilizing the inside and the outside of a CO ₂ incubator having a tube connection portion communicating with the inside. A sterilization tent having an airtight hole for airtightly projecting the tip of the part to the outside and a discharge part for discharging ozone gas to the outside, and covering the CO ₂ incubator in an airtight manner, and connecting ozone gas to the tube An ozone gas generator that circulates and sterilizes the inside and outside of the CO ₂ incubator covered by the sterilization tent by pumping to the part, and ozone gas that neutralizes and eliminates the ozone gas discharged from the discharge part And sum means.
Further, the ozone sterilization apparatus for the CO ₂ incubator according to the second invention is the ozone sterilization apparatus for the CO ₂ incubator according to the first invention, wherein the packing is pressed against the outer peripheral surface of the tube connecting portion in the airtight hole. A member is provided.
The third ozone sterilizer of CO ₂ incubator according to the invention, the ozone sterilizer in CO ₂ incubator according to the first or second invention, the sterilization tent has a plurality of the airtight bore, the Each of the plurality of airtight holes can be sealed.

Further, a fourth ozone sterilizer of CO ₂ incubator according to the invention, the ozone sterilizer in CO ₂ incubator according to any one of the invention the first to third one, the ozone gas neutralizing means, the activated carbon filter It is characterized by becoming.
Furthermore, a fifth ozone sterilizer of CO ₂ incubator according to the invention, the ozone sterilizer in CO ₂ incubator according to any one of the invention the first to fourth one, pumping sterile air into the tube connecting portion Aseptic air supply means for discharging the residual gas remaining in the CO ₂ incubator and in the sterilization tent from the discharge unit, and measuring the residual ozone concentration in the residual gas discharged from the discharge unit And an ozone measuring device.

The ozone sterilizer for a CO ₂ incubator according to the present invention covers a CO ₂ incubator with a sterilization tent that covers the entire CO ₂ incubator in an airtight manner, and also feeds and circulates ozone gas into the CO ₂ incubator through a tube connection portion. ₂ The primary sterilization inside the incubator was performed, and then the ozone gas inside the CO ₂ incubator was discharged and circulated inside the sterilization tent so that the outer peripheral surface of the CO ₂ incubator was subjected to secondary sterilization. It is possible to completely sterilize the entire CO ₂ incubator with a minimum scale and a relatively small amount of ozone gas as compared with a case where a clean room is configured in units of a room and ozone gas is filled and sterilized throughout the room. it can.

Moreover, even if ozone gas sent into the CO ₂ incubator during the first sterilization leaks, there is no danger because it stays inside the sterilization tent, and not only the inside of the CO ₂ incubator but also the secondary sterilization of the CO ₂ incubator Since external surface sterilization can also be performed, sterilization before and after the use of the CO ₂ incubator can be performed almost completely.

In addition, after sterilizing the inside and outside of the CO ₂ incubator, the ozone gas is discharged to the outside of the sterilization tent and neutralized, and the sterile air is pumped to the tube connection portion, thereby the inside of the CO ₂ incubator and the inside of the sterilization tent. And the aseptic air supply means for discharging the residual gas remaining in the exhaust gas, the discharged ozone can be quickly neutralized and eliminated.

Further, by having an ozone measuring device for measuring the ozone residual concentration in the discharged residual gas, the concentration of ozone gas were pumped through the inside of the CO ₂ incubator can be monitored at all times, whereby the interior of the CO ₂ incubator ozone It is easy to finely adjust the ozone gas concentration by constantly checking the concentration and pursuing ozone gas as necessary or reducing the concentration.

Overall perspective view that shows the outline of the sterilization tent used as an ozone sterilization means CO ₂ incubator according to an embodiment of the present invention. The explanatory view showing the outline of the whole ozone sterilization system of the CO ₂ incubator of the present invention centering on the sterilization tent of FIG. 1 (during sterility test and sterilization confirmation inspection). The explanatory view showing the outline of the whole ozone sterilization system of the CO ₂ incubator of the present invention centering on the sterilization tent of FIG. 1 (primary sterilization in the CO ₂ incubator). Explanatory drawing showing the outline of the whole ozone sterilization system in the CO ₂ incubator of the present invention centering on the sterilization tent of FIG. 1 [CO ₂ incubator internal (primary) and external (secondary) sterilization]. The explanatory view (ozone discharge) showing the outline of the whole ozone sterilization system of the CO ₂ incubator of the present invention centering on the sterilization tent of FIG.

The contents of the present invention will be described below based on an embodiment shown in FIG. In the figure, 1 is a sterilization tent that covers the entire CO ₂ incubator in an airtight manner, 14 and 9 are tube connections, 18 is an ozone supply source, 20 is a filtration filter, and constitutes a sterile air supply source together with an air pump 21. Yes. Furthermore, 23 is an ozone measuring device for measuring the ozone gas concentration, 26 is an activated carbon filter as ozone neutralizing means, 28 is an ozone concentration measuring device for measuring the ozone gas concentration, and 30 is a sampling box.

The sterilization tent 1 has a sheet having a function of not allowing ozone gas to permeate, including thick polyethylene, vinyl chloride and other resins, and the entire CO ₂ incubator is composed of a front portion 2 and left and right side portions 3 (the opposite sides in the figure). The side portion is not shown), the ceiling portion 4, the back portion (not shown), etc., and the like can be covered airtightly with a margin and a small room shape.

The size of the sterilization tent 1 may be a small room size that can cover the entire CO ₂ incubator with a sufficient margin as described above, but there is not much room for the entire volume of the CO ₂ incubator. If the volume is not enough, the gap between the outer surface of the CO ₂ incubator is reduced and sufficient circulation of ozone gas cannot be obtained. Therefore, the volume of the CO ₂ incubator is at least 1.3 times larger than the total volume. Is preferred.

Moreover, if the volume of the sterilization tent 1 is too large compared to the total volume of the CO ₂ incubator, the amount of ozone gas used increases and the cost increases, and the efficiency of the sterilization operation decreases, and the installation area increases. Thus, according to the results of various experiments conducted by the present inventors, it has become clear that the total volume of the CO ₂ incubator is preferably 4 times or less, and more preferably 3 times or less. The front part 2, the left and right side parts 3, and the bottom parts of the back part of the sterilization tent 1 need to be configured to be in airtight contact with the floor surface on which the CO ₂ incubator 11 is installed.

In this case, if necessary, the front portion 2, the left and right side portions 3, and the bottom portions of the rear portion are sealed with airtight fasteners (not shown) [for example, "Aqua Seal" (YKK Co., Ltd.) (hereinafter the same)]. In this case, it is necessary to install the CO ₂ incubator 11 on the floor sheet in a state where the bottom sheet is previously laid on the floor surface.

Further, the front part 2 of the sterilization tent 1 forms an opening 5 by cutting near the left and right sides while leaving a part near the floor, and leaves a part near the upper end, and continues from the cutting edges 7 near the left and right sides. A front portion opening / closing sheet 6 that opens or closes the opening portion 5 of the front portion 2 by an airtight fastener 7a attached along the lower horizontal cutting edge is provided. Further, in FIG. 1, reference numeral 10 denotes a bracket for passing a string or a thin rope-like object to suspend the entire sterilization tent 1 from the top surface of the CO ₂ incubator 11.

More lower portion of the positive surface portion closing sheet 6, when closed by gas-tight fastener 7a a positive face-off sheet 6, the front side in the lower portion of the front side of the door 12 and control panel 13 of the CO ₂ incubator 11 The tube connection portions 14a and 14b communicated with the inside of the CO ₂ incubator 11 are attached to the tube ₂ so that the projecting tips of the tube connection portions 14a and 14b protrude outwardly from the sterilization tent 1 in an airtight manner. Airtight holes 8a and 8b that can be made are formed. It is to be noted that a packing member that can be press-contacted to the outer peripheral surfaces of the tube connecting portions 14a and 14b may be fitted into the airtight holes 8a and 8b.

Note the bottom of the CO ₂ incubator 11, for the tube connecting portion 14a · 14b mounted by communicating the one end therein, it is only one of the tube connecting portion 14a or 14b, but damage to the connecting portion of the tube connecting portion In the present embodiment, a spare tube connecting portion is provided for the case where a malfunction such as the above occurs, and the spare connecting portion 9b other than the normally used tube connecting portion 9a is sealed at the opening. .

Further, near the upper end portion of the front portion 2 of the sterilization tent 1, tube connection portions 9a and 9b having one end communicating with the inside of the sterilization tent 1 are projected outward. In this case, the tube connection part normally used may be either one of 9a or 9b, but a spare tube connection part is provided in preparation for a case such as breakage in the connection part of the tube connection part, The spare connection portion 9b other than the tube connection portion 9a that is normally used is sealed at the opening. Incidentally 12a has represents an exhaust hole leading to the CO ₂ incubator 11 of was formed above the front door 12 of the CO ₂ incubator 11.

  On the other hand, a tube 15 is connected to the tube connecting portion 14 a, and an ozone supply source 18 is attached to the tube 15 via three-way valves 16 and 17. In this embodiment, a general-purpose ozone generator (for example, “ED-OG-AP1” manufactured by Ecodesign Co., Ltd.) is used as an ozone supply source. Further, a filtration filter 20 and an air pump 21 are attached to the three-way valve 16 on the way through a tube 19, and the filtration filter 20 and the air pump 21 constitute a sterile air supply source.

  As for the filtration filter 20 described above, a Millex-FG (pore size: 0.22 μm) manufactured by Millipore is used in this embodiment. The three-way valve 17 is provided with an ozone measuring device 23 for measuring the ozone concentration via a tube 22. As the ozone measuring device 23 in this case, for example, “UV-100” of NK Scientific Hook is used.

  Furthermore, an activated carbon filter 26 that is an ozone neutralizing means is provided in the tube connecting portion 9 a through the tube 24. Further, three-way valves 25 and 31 are interposed in the middle of the tube 24, and an ozone measuring device (with a built-in pump) 28 for measuring the ozone concentration is attached to one of the three-way valves 25 through the tube 27, and the other A sampling box 30 is attached to the three-way valve 31 via a tube 29.

Sampling box 30, or by opening and closing the door 12 of the CO ₂ incubator 11 by remaining bacteria circulated within gas out of the CO ₂ incubator 11 to test whether mixed or separately sterilized confirmation means This is a device for confirming the sterilization inside and outside of the CO ₂ incubator 11 safely and surely without requiring a method such as installing the CO ₂ .

  As for the sampling box 30 used here, in a specific example, the inside of a small covered box connected to the tube 29 is sterilized by a method such as wiping with a cotton swab preliminarily containing alcohol to make a sterile space. A sterile medium such as an agar medium, a liquid medium, a film-shaped medium, or the like is prepared, and exhaust gas is blown from the tube 29 to the aseptic medium installed in the sampling box 30 to thereby discharge exhaust gas. If residual bacteria remain inside, the bacteria adhere to the medium. By observing this medium for a certain period of time, it is confirmed that the bacteria have propagated when the bacteria are attached, and that no remaining bacteria are present when the bacteria have not propagated.

When using the CO ₂ incubator 11 or when sterilizing the CO ₂ incubator 11 after use, the CO ₂ incubator is hermetically covered with the sterilization tent 1 and ozone gas is put into the CO ₂ incubator 11 for a certain period of time. While sterilizing, the ozone gas in the CO ₂ incubator is then discharged into the CO ₂ sterilization tent 1 and circulated around the outer periphery of the incubator to sufficiently sterilize both the inside and outside of the CO ₂ incubator, and then sterile air is fed The sterility test can be performed after ozone gas is forcibly discharged and extinguished.

In the configuration of the above-described embodiment, a specific procedure of sterilization work will be described below. In this case, the entire CO ₂ incubator 11 must be airtightly covered with the sterilization tent 1 in advance.

[Sterility test]
When using the CO ₂ incubator 11, as shown in FIG. 2, the three-way valve 16 is operated, the three-way valve 17 side is closed, and the air pump 21 is started with the tube 19 communicated with the tube 15. Then, the air is sucked into the atmosphere and sterilized through the filter 20 is sent from the tube connection portion 14 a into the CO ₂ incubator 11 to sufficiently circulate the sterile air in the CO ₂ incubator 11.

When the sterilized air continues to be fed, the air circulated in the CO ₂ incubator 11 is sequentially discharged from the upper exhaust hole 12a and circulates inside the sterilization tent 1 (the outer surface of the CO ₂ incubator 11). Thereafter, the three-way valve 25 is operated to shut off the tube 27 side, and the three-way valve 31 is operated to close the tube 24 on the activated carbon filter 26 side so that the tube 29 is communicated with the tube 24, and the CO ₂ incubator 11. The air exhausted from the tube connecting portion 9a above is exhausted into the sampling box 30 through the tube 24 and the tube 29, and the presence or absence of bacteria in the exhaust gas is confirmed. This sterility test is the same when it is performed after the use of the CO ₂ incubator 11.

[Sterilization operation]
As for the sterilization operation, the three-way valves 16 and 17 in FIG. 3 are operated to cut off the communication with the tube 19 and the ozone generator 18 is communicated with the tube connecting portion 14a of the CO ₂ incubator 11 via the tube 15. after, feeding the ozone generated by operating the ozone generator 18 in the CO ₂ incubator 11 from the lower portion of the CO ₂ incubator 11, to sufficiently circulate the internal (primary sterilization).

By continuing to send ozone, the ozone gas pressure in the CO ₂ incubator 11 gradually increases, and as shown in FIG. 4, the ozone gas is automatically discharged sequentially from the upper exhaust hole 12a to the inside of the sterilization tent 1 (CO ₂ incubator 11 (Secondary sterilization). By this primary sterilization and secondary sterilization, the sterilization operation of both the inside and the outer peripheral surface of the CO ₂ incubator 11 is performed.

  In the above sterilization process, the concentration of ozone can be measured as necessary. In this case, the tube 15 is also communicated with the tube 22 via the three-way valve 17, the ozone gas passing through the tube 15 is guided to the ozone measuring device 23 via the tube 22, and the concentration of ozone flowing through the tube 15 is measured. If it is insufficient or excessive, the operation of the ozone generator 18 can be adjusted and adjusted.

The CT value is mainly used for the ozone sterilization operation, and the ozone concentration can be expressed as PPM × min. Assumed ozone concentration for completing the sterilization operation is set to, for example, CT: 10,000 or more. In addition, as a sterilization measurement means used in this case, for example, a chemical indicator (for example, an ozone indicator) is used, which can be sequentially confirmed by installing it at a plurality of locations inside the CO ₂ incubator 11.

In addition, a biological indicator can be used as the sterilization measuring means. In this case, it is necessary to increase the humidity during use, and from the inside of the CO ₂ incubator 11 when checking the CT value. Since it is necessary to take out outside, there is a case where the adhesion of the bacteria at that time is suspected, which is not preferable.

[Ozone emission]
As shown in FIG. 5, ozone discharge is performed by operating the three-way valve 16 to shut off the three-way valve 17 side, and connecting the tube 19 to the tube 15 and starting the air pump 21 to filter the intake air. The air sterilized by passing the air is pumped into the CO ₂ incubator 11 from the lower tube connecting portion 14a.

If sterilization air is continuously injected, the sterilization air is automatically discharged sequentially from the upper exhaust hole 12a into the sterilization tent 1 along with the ozone gas remaining in the CO ₂ incubator 11, and circulates in the sterilization tent 1. The residual ozone gas in the sterilization tent 1 is sequentially discharged to the outside from the upper tube connection portion 9a, and by continuing this, the residual ozone gas in the CO ₂ incubator 11 and the sterilization tent 1 are gradually discharged. .

  In this process, it is possible to measure the ozone concentration by measuring the ozone concentration by introducing the exhaust gas into the ozone measuring device 28 through the tube 27 communicated through the three-way valve 25 at any time. When the concentration of ozone gas becomes equal to that of outside air, it is possible to confirm the discharge of ozone gas.

The residual ozone gas in the CO ₂ incubator 11 and the sterilized tent 1 sequentially discharged by the injection of aseptic air passes through the three-way valves 25 and 31 and is sent into the activated carbon filter 26 by the tube 24, where it is neutralized. After discharged.

[Sterilization confirmation inspection]
After the use of the CO ₂ incubator 11, sterilization confirmation work is performed by the same method as the sterility test before use. That is, as shown in FIG. 2 described above, the three-way valve 16 is operated to shut off the tube 15 in the direction of the three-way valve 17, and the three-way valve 25 is further operated to shut off the tube 27 in the direction of the ozone measuring device 28. The valve 31 is operated so that the tube 24 in the direction of the activated carbon filter 26 is shut off. In this state, the air sucked by the air pump 21 is sterilized via the filter 20 and continuously passed through the tubes 19 and 15. Circulate from the lower tube connection portion 14 a to the inside and outside of the CO ₂ incubator 11 (inside the sterilization tent 1), and further to the outside from the tube connection portion 9 a above the sterilization tent 1, through the tubes 24 and 29. The sample is sent into the sampling box 30 and the presence or absence of bacteria in the exhaust gas is confirmed (see FIG. 5).

  The operations of the three-way valves 16, 17, 25, and 31 at the time of each of the above-described [sterility test], [sterilization operation], [ozone discharge], and [sterilization confirmation inspection] are manually performed each time, or particularly illustrated. Although not, it is also possible to operate electrically according to an electronic memory circuit with a predetermined pattern.

1 sterile tent 2 front portion 3 left and right both side surface portions 4 cut ceiling 5 opening 6 front opening and closing the sheet 7 edge 7a airtight fastener 8a airtight hole 8b airtight bore 9a tube connecting portion 9b tube connecting portion 10 the bracket 11 CO ₂ incubator 12 opening Door 12a Exhaust hole 13 Operation panel 14a Tube connection portion 14b Tube connection portion 15 Tube 16 Three-way valve 17 Three-way valve 18 Ozone generator 19 Tube 20 Filtration filter 21 Air pump 22 Tube 23 Ozone meter 24 Tube 25 Three-way valve 26 Activated carbon filter 27 Tube 28 Ozone measuring device 29 Tube 30 Sampling box 31 Three-way valve

Claims (5)

An ozone sterilization apparatus for circulating and sterilizing the inside and outside of a CO ₂ incubator having a tube connection portion communicating with the inside,
A sterilization tent having an airtight hole for airtightly projecting the distal end portion of the tube connection portion to the outside, and a discharge portion for discharging ozone gas to the outside, and covering the CO ₂ incubator in an airtight manner;
An ozone gas generator for circulating and sterilizing the inside and outside of the CO ₂ incubator covered by the sterilization tent by pumping ozone gas to the tube connection part;
And an ozone gas neutralizing means for neutralizing and eliminating the ozone gas discharged from the discharge section. An ozone sterilization apparatus for a CO ₂ incubator, comprising: _2. The ozone sterilization apparatus for a CO ₂ incubator according to claim 1, wherein a packing member pressed against an outer peripheral surface of the tube connecting portion is disposed in the airtight hole. The sterilization tent has a plurality of the airtight holes,
_3. The ozone sterilization apparatus for a CO ₂ incubator according to claim 1, wherein each of the plurality of airtight holes can be sealed. The ozone sterilization apparatus for a CO ₂ incubator according to any one of claims 1 to 3, wherein the ozone gas neutralizing means comprises an activated carbon filter. Aseptic air supply means for discharging the residual gas remaining in the CO ₂ incubator and the sterilization tent from the discharge part by pumping sterile air to the tube connection part,
An ozone sterilizer for a CO ₂ incubator according to any one of claims 1 to 4, further comprising: an ozone measuring device that measures a residual ozone concentration in the residual gas discharged from the discharge unit. .

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