JP2012198225A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2012198225A5 JP2012198225A5 JP2012110792A JP2012110792A JP2012198225A5 JP 2012198225 A5 JP2012198225 A5 JP 2012198225A5 JP 2012110792 A JP2012110792 A JP 2012110792A JP 2012110792 A JP2012110792 A JP 2012110792A JP 2012198225 A5 JP2012198225 A5 JP 2012198225A5
- Authority
- JP
- Japan
- Prior art keywords
- target molecule
- oligonucleotide
- population
- oligonucleotide population
- sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 108091034117 Oligonucleotide Proteins 0.000 claims 28
- 238000000034 method Methods 0.000 claims 18
- 108091008104 nucleic acid aptamers Proteins 0.000 claims 7
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims 3
- 238000001514 detection method Methods 0.000 claims 2
- 125000003729 nucleotide group Chemical group 0.000 claims 2
- 238000012163 sequencing technique Methods 0.000 claims 2
- 108090000790 Enzymes Proteins 0.000 claims 1
- 102000004190 Enzymes Human genes 0.000 claims 1
- 150000001413 amino acids Chemical class 0.000 claims 1
- 230000000295 complement effect Effects 0.000 claims 1
- 150000002632 lipids Chemical class 0.000 claims 1
- 230000011987 methylation Effects 0.000 claims 1
- 238000007069 methylation reaction Methods 0.000 claims 1
- 230000004048 modification Effects 0.000 claims 1
- 238000012986 modification Methods 0.000 claims 1
- 229920000768 polyamine Polymers 0.000 claims 1
- 108090000765 processed proteins & peptides Proteins 0.000 claims 1
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2012110792A JP2012198225A (ja) | 2010-07-01 | 2012-05-14 | 標的分子の検出法 |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2010151242 | 2010-07-01 | ||
| JP2010151242 | 2010-07-01 | ||
| JP2012110792A JP2012198225A (ja) | 2010-07-01 | 2012-05-14 | 標的分子の検出法 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011548712A Division JPWO2012002541A1 (ja) | 2010-07-01 | 2011-07-01 | 標的分子の検出法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2012198225A JP2012198225A (ja) | 2012-10-18 |
| JP2012198225A5 true JP2012198225A5 (enExample) | 2014-02-13 |
Family
ID=45402243
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011548712A Pending JPWO2012002541A1 (ja) | 2010-07-01 | 2011-07-01 | 標的分子の検出法 |
| JP2012110792A Pending JP2012198225A (ja) | 2010-07-01 | 2012-05-14 | 標的分子の検出法 |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011548712A Pending JPWO2012002541A1 (ja) | 2010-07-01 | 2011-07-01 | 標的分子の検出法 |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US20130116129A1 (enExample) |
| EP (1) | EP2589657A4 (enExample) |
| JP (2) | JPWO2012002541A1 (enExample) |
| WO (1) | WO2012002541A1 (enExample) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5835778B2 (ja) * | 2010-07-02 | 2015-12-24 | 国立大学法人東京農工大学 | Ｐｓａ結合アプタマー及びｐｓａ検出用キット |
| JP6183917B2 (ja) * | 2012-03-23 | 2017-08-23 | Ｎｅｃソリューションイノベータ株式会社 | ストレプトアビジンの分析用デバイスおよび分析方法 |
| JP5999786B2 (ja) | 2012-03-23 | 2016-09-28 | Ｎｅｃソリューションイノベータ株式会社 | Ａｔｐまたはａｍｐの分析用デバイスおよび分析方法 |
| US20150292005A1 (en) * | 2012-04-27 | 2015-10-15 | Kirin Kabushiki Kaisha | Nucleic acid molecules for highly sensitive detection of ligands, screening method for nucleic acid molecules, and optimization method for sensitivity of nucleic acid molecules |
| AU2013340414B2 (en) | 2012-10-23 | 2019-05-02 | Caris Science, Inc. | Aptamers and uses thereof |
| US10942184B2 (en) | 2012-10-23 | 2021-03-09 | Caris Science, Inc. | Aptamers and uses thereof |
| EP2935628B1 (en) | 2012-12-19 | 2018-03-21 | Caris Life Sciences Switzerland Holdings GmbH | Compositions and methods for aptamer screening |
| US9828599B2 (en) * | 2013-09-18 | 2017-11-28 | General Electric Company | Methods of selecting binding-elements and uses thereof |
| US10359419B2 (en) | 2013-10-02 | 2019-07-23 | General Electric Company | Methods for detection of target using affinity binding |
| JP6128621B2 (ja) * | 2013-12-04 | 2017-05-17 | 国立研究開発法人産業技術総合研究所 | マイクロベシクルに対する核酸アプタマー |
| CN105483267B (zh) * | 2016-01-15 | 2018-12-04 | 古博 | 血浆游离DNA双分子标记、标记和检测血浆cfDNA的方法及其用途 |
| US20220396825A1 (en) | 2019-11-15 | 2022-12-15 | Kao Corporation | Method for preparing sequencing library |
| JP7146839B2 (ja) * | 2019-11-15 | 2022-10-04 | 花王株式会社 | シーケンシング用ライブラリの調製方法 |
| CN113699215B (zh) * | 2021-08-13 | 2024-06-14 | 东南大学 | 一种核酸适体筛选方法 |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5874218A (en) * | 1990-06-11 | 1999-02-23 | Nexstar Pharmaceuticals, Inc. | Method for detecting a target compound in a substance using a nucleic acid ligand |
| US5270163A (en) | 1990-06-11 | 1993-12-14 | University Research Corporation | Methods for identifying nucleic acid ligands |
| KR970002255B1 (ko) * | 1990-06-11 | 1997-02-26 | 넥스스타 파아마슈티컬드, 인크. | 핵산 리간드 |
| US20090053138A1 (en) * | 2002-11-21 | 2009-02-26 | Preiss Jeffrey R | Stabilized Aptamers to Platelet Derived Growth Factor and their Use as Oncology Therapeutics |
| JP4841396B2 (ja) * | 2006-10-18 | 2011-12-21 | Ｎｅｃソフト株式会社 | 塩基配列の同定装置、核酸分子の二次構造取得装置、塩基配列の同定方法、核酸分子の二次構造取得方法、プログラム及び記録媒体 |
| JP2008161165A (ja) | 2006-12-28 | 2008-07-17 | Dnaform:Kk | 競合オリゴヌクレオチドを用いた遺伝子検出法 |
| US20100298152A1 (en) * | 2007-10-12 | 2010-11-25 | Pronota Nv | Use of aptamers in proteomics |
| JP2011500076A (ja) * | 2007-10-22 | 2011-01-06 | プロノタ・エヌブイ | アプタマーを選択する方法 |
| JP2009189310A (ja) * | 2008-02-15 | 2009-08-27 | Olympus Corp | 高親和性核酸フラグメント群の塩基配列決定方法および核酸アプタマーのスクリーニング方法 |
| JP5463621B2 (ja) * | 2008-02-27 | 2014-04-09 | ソニー株式会社 | 標的物質の定量方法 |
| US20110263459A1 (en) * | 2008-03-12 | 2011-10-27 | Borer Philip N | Direct selection of structurally defined aptamers |
| JP2010041951A (ja) | 2008-08-11 | 2010-02-25 | Olympus Corp | 標的分子の検出方法 |
| WO2010019969A1 (en) * | 2008-08-15 | 2010-02-18 | Cornell University | Device for rapid identification of nucleic acids for binding to specific chemical targets |
| JP5256016B2 (ja) | 2008-12-25 | 2013-08-07 | コスモ工機株式会社 | 管接続部材の脱着装置 |
| JP5419145B2 (ja) * | 2009-07-24 | 2014-02-19 | Ｎｅｃソフト株式会社 | アプタマー分類装置、アプタマー分類方法、プログラムおよび記録媒体 |
-
2011
- 2011-07-01 EP EP11801003.2A patent/EP2589657A4/en not_active Withdrawn
- 2011-07-01 JP JP2011548712A patent/JPWO2012002541A1/ja active Pending
- 2011-07-01 US US13/807,501 patent/US20130116129A1/en not_active Abandoned
- 2011-07-01 WO PCT/JP2011/065205 patent/WO2012002541A1/ja not_active Ceased
-
2012
- 2012-05-14 JP JP2012110792A patent/JP2012198225A/ja active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2012198225A5 (enExample) | ||
| Kohlberger et al. | SELEX: Critical factors and optimization strategies for successful aptamer selection | |
| Stoltenburg et al. | Capture‐SELEX: selection of DNA aptamers for aminoglycoside antibiotics | |
| Kim et al. | Advances in aptamer screening and small molecule aptasensors | |
| Stoltenburg et al. | FluMag-SELEX as an advantageous method for DNA aptamer selection | |
| JP2023071981A (ja) | 酵素不要及び増幅不要の配列決定 | |
| Briones et al. | Applications of peptide nucleic acids (PNAs) and locked nucleic acids (LNAs) in biosensor development | |
| JP2017535269A5 (enExample) | ||
| JP2017532042A5 (enExample) | ||
| JP2013507642A5 (enExample) | ||
| US20130116129A1 (en) | Method for detecting target molecules | |
| WO2011097528A1 (en) | Methods and compositions for profiling rna molecules | |
| JP2016515827A5 (enExample) | ||
| Yüce et al. | Systematic evolution of ligands by exponential enrichment for aptamer selection | |
| WO2011105071A1 (en) | Method for screening nucleic acid ligand | |
| JP2013524841A (ja) | 一本鎖オーバーハングを有する二本鎖核酸を生成するための方法 | |
| JP6831776B2 (ja) | ２個のリガンドと１個のレセプタとからなる高親和性錯体の特定方法と該方法を実行する装置及び該方法に用いられる自己集合型ケミカルライブラリ | |
| AU2017312947A1 (en) | High throughput oil-emulsion synthesis of bowtie barcodes for paired mrna capture and sequencing from individual cells | |
| US9499814B2 (en) | Methods for identifying drug effects on a cell by determining changes in the cell's spliced message profile | |
| KR102664887B1 (ko) | 핵산 라이브러리의 정제방법 | |
| KR101274118B1 (ko) | 리스테리아 모노사이토제네스 생균의 표면에 특이적으로 결합하는 ｄｎａ 앱타머 및 이의 용도 | |
| JP7076139B2 (ja) | Ｒｎａ構造ライブラリ | |
| Dausse et al. | In vitro selection procedures for identifying DNA and RNA aptamers targeted to nucleic acids and proteins | |
| FI4077713T3 (fi) | Menetelmä ja kitti kokogenomiamplifikaatioon ja biologisessa näytteessä olevien kohdemolekyylien analysointiin | |
| WO2012156718A1 (en) | Method of immobilising rna onto a surface |