JP2012136447A - Lipase inhibitor, and cosmetic, food/drink composition and pharmaceutical composition each containing the same - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a stable and inexpensive lipase inhibitor of high safety, excellent in prophylactic and ameliorative effects and curing effect for pimples caused by normal bacteria flora on the skin such as acne bacteria and excellent in the prophylactic and ameliorative effects and curing effects for lifestyle-related diseases including obesity, hyperlipemia and diabetes each induced by high-fat meals in daily life.SOLUTION: The lipase inhibitor includes 1-isomangosteen as an active ingredient. Besides, cosmetics, food/drink compositions and pharmaceutical compositions each containing the lipase inhibitor are also provided.

Description

The present invention relates to a lipase inhibitor, and in particular, is excellent in preventing and treating acne due to skin resident bacteria such as acne bacteria, and is a lifestyle habit such as obesity, hyperlipidemia and diabetes induced by a high fat diet. The present invention relates to a lipase inhibitor that is excellent in preventing and treating diseases and is effective, stable, inexpensive, and safe. Moreover, it is related with the cosmetics, food-drinks composition, and pharmaceutical composition containing this lipase inhibitor.

In recent years, with the westernization of meals, the onset of lifestyle-related diseases such as obesity, hyperlipidemia and diabetes has increased remarkably, and the response is urgently needed. In order to prevent and improve obesity, hyperlipidemia or diabetes caused by diet, lipase inhibitors have been actively developed and applied. Moreover, it has been clarified that lipase inhibitors are also useful for preventing and improving acne caused by skin resident bacteria such as acne bacteria.

In contrast, various lipase inhibitors extracted from plant fruits, their skin, seeds, leaves, rhizomes, etc. have been manufactured and tried to be incorporated into cosmetics, pharmaceuticals, foods, etc. In some cases, the safety and procurement of the raw materials are problematic.

Lipase inhibitors derived from plant materials include lipase inhibitors containing extract extracts such as yucca, ginseng, jasmine tea, yamakoko, yellow ginger tea, rooibos tea, soybean germ, ginger, and chuchu tea (patent document) 1), pancreatic lipase inhibitor (see Patent Document 2) containing an extract of Quillaja saponaria MOLINA bark as an active ingredient, and a lipase inhibitor (see Patent Document 3) containing chestnut skin extract. is there.

On the other hand, Mangosteen (Garcinia mangostana L.) belonging to the genus Hypericaceae is also known as a plant-derived raw material. There are various agents in which an ingredient derived from mangosteen effectively acts, for example, a composition comprising a concentrated extract of mangosteen peel contains xanthone and tannin, sunburn, photosensitivity, skin-localization Be a therapeutic agent sufficient for the prevention or treatment of skin diseases such as allergies, immunosuppression, premature aging, psoriasis, cancerous injury, precancerous injury, bacterial infection, viral infection, fungal infection and rash (e.g. Patent Document 4) is known. In addition, specific xanthone derivatives have an anti-Helicobacter pylori action (for example, see Patent Document 5 or Patent Document 6), and have a bactericidal action against Gram-positive bacteria (for example, see Patent Document 7). ), Those having a topoisomerase I and II inhibitory activity (for example, see Patent Document 8), those having a platelet activating factor inhibitory action (for example, see Patent Document 9), those having an antioxidant action (for example, Patent Document 10 or Patent Document 11), those having a whitening effect (for example, see Patent Document 12), those having a collagen increasing effect (for example, see Patent Document 13), and the like. Moreover, there is an oral cleansing liquid (for example, refer to Patent Document 14) containing a xanthone derivative mixture containing α-mangostin.

JP 2002-275077 A JP 2006-182722 A JP 2007-145802 A Special table 2008-520585 gazette JP-A-8-217672 JP-A-8-231396 Japanese Patent Laid-Open No. 9-110688 Japanese Patent Laid-Open No. 10-203777 JP 2004-137210 A JP 2006-89661 A JP 2009-13104 A JP 2007-153773 A JP 2009-13105 A JP 2010-18551 A

As in the above background art, it has been known that components derived from mangosteen have various actions, but it has not been known that 1-isomangosteen has an action to inhibit lipase activity.

The object of the present invention is excellent in preventing and improving acne caused by resident skin bacteria such as acne, and is effective in treating and preventing obesity, hyperlipidemia and diabetes induced by a high fat diet in daily life. An object of the present invention is to provide a lipase inhibitor that is excellent in preventing and treating lifestyle-related diseases, is stable, inexpensive, and has excellent safety. Another object is to provide a cosmetic, a food / beverage composition and a pharmaceutical composition containing the lipase inhibitor.

As a result of intensive studies in view of the above circumstances, the present inventors have found that 1-isomangostin has an action of inhibiting lipase derived from pancreas and lipase derived from acne bacteria, and further, 1-isomangosteen is used as a cosmetic. They were found to have a remarkable effect on prevention and improvement and treatment of various skin troubles and lifestyle-related diseases caused in daily life when formulated in food and beverage compositions and pharmaceutical compositions.

That is, the lipase inhibitor of the present invention is characterized by containing 1-isomangostin as an active ingredient. Furthermore, this invention is also cosmetics, food-drinks composition, and pharmaceutical composition containing the said lipase inhibitor.

According to the present invention, it is excellent in preventing and improving acne caused by skin resident bacteria such as acne, and is effective in treating and preventing obesity, hyperlipidemia and diabetes induced by a high-fat diet in daily life. It is possible to provide a lipase inhibitor which is excellent in preventing and treating lifestyle-related diseases and is stable, inexpensive and excellent in safety. And this lipase inhibitor is suitable for blending in cosmetics, food and beverage compositions and pharmaceutical compositions, and these are also excellent in preventing and improving acne caused by skin resident bacteria such as acne bacteria, Excellent in preventing and treating lifestyle-related diseases such as obesity, hyperlipidemia and diabetes induced by a high fat diet in daily life.

Hereinafter, the present invention will be described in detail.

1-Isomangostin used for the lipase inhibitor of the present invention is a xanthone compound represented by the following general formula.

1-Isomangosteen is also contained in mangosteen fruit hulls, and can also be obtained, for example, by solvent extraction from mangosteen hulls. 1-Isomangostin may be a commercially available product, for example, “1-Isomangostin” from Sichuan Weikeqi Biological Technology Co., Ltd, “1-Isomangosteen” from Chem Blink Inc. . In the case of solvent extraction from mangosteen rind, the method is not particularly limited, but when extracting with a solvent, mangosteen rind may be used after drying or without drying. Further, the mangosteen rind may be used as it is regardless of drying, or may be used after being finely cut or pulverized. From the viewpoint of extraction efficiency, it is preferable to perform solvent extraction using a powdered mangosteen skin after drying. It is desirable that the solvent used for solvent extraction of 1-isomangosteen is dissolved in water, a water-soluble organic solvent, or a mixed solvent thereof. Examples of the water-soluble organic solvent include lower alcohols such as methanol, ethanol, propanol, n-butanol, and 1,3-butylene glycol, benzene, ethyl ether, chloroform, ethyl acetate, butyl acetate, and acetone. Among these, ethanol is preferable, and water-containing ethanol which is a mixed solvent with water is particularly preferable because of its strong lipase inhibitory action.

The apparatus used at the time of solvent extraction is not specifically limited, A normal tank and a mixer may be used, and extractors, such as a Soxhlet extractor, may be used. The temperature at the time of extraction and the extraction time are not particularly limited. After solvent extraction, impurities may be removed by filtration, treatment with an adsorbent resin, activated carbon treatment, or the like. Furthermore, 1-isomangostin can be fractionated at a temperature of 25 ° C. using a column YMC-Pack C8 (SH-243-5, manufactured by YMC Co., Ltd.) and a fluid phase (75% acetonitrile) by liquid high performance chromatography. it can.

As the dosage form of the lipase inhibitor of the present invention, in addition to the liquid extract obtained by solvent extraction, the liquid extract was dried or concentrated by a normal drying method such as reduced pressure drying or freeze drying or a concentration method. It may be a thing. In addition, if necessary, it may be used after purification treatment such as deodorization and decoloration within a range that does not affect its efficacy, and it is formulated in a state that is easy to use, such as granulated with an appropriate excipient. What is necessary is just to use.

The lipase inhibitor of the present invention includes, in addition to 1-isomangostin, components that are usually used in cosmetics, food and beverage compositions or pharmaceutical compositions, or components that are allowed to be used, depending on the purpose. It can mix | blend suitably within the range which does not impair the effect.

The blending amount of 1-isomangostin in the cosmetic, food / beverage composition or pharmaceutical composition containing the lipase inhibitor of the present invention is based on the total amount of the cosmetic, the food / beverage composition or the pharmaceutical composition, and the dry solid content. 0.005 wt% (abbreviation of mass%) or more and 10 wt% or less in terms of conversion is preferable from the viewpoint of the effect and cost. In particular, 0.005 wt% or more and 5 wt% or less is preferable when blended and applied to a product.

The cosmetics of the present invention are not particularly limited, and examples thereof include skin cosmetics, bath cosmetics, cleaning cosmetics, slimming cosmetics, acne cosmetics, and the forms thereof are emulsions, gels, sticks. , Sheets, pops, gels, powders, liquids, granules, creams, pastes, solids, and the like. These cosmetics can be manufactured by a conventional method, and if necessary, generally used in cosmetics such as excipients and fragrances, fats and oils, surfactants, moisturizers, whitening agents, pH Conditioning agents, binders, polyhydric alcohols, essential oils and fragrances, thickeners, preservatives, antioxidants, UV absorbers, pigments, plant pulverized products and herbal medicines, inorganic salts and inorganic acids, cleaning agents Various cosmetic ingredients such as emulsifiers can be appropriately blended.

The type of the food / beverage composition of the present invention is not particularly limited, and examples thereof include confectionery (chewing gum, candy, gummi, tablet, chocolate, jelly, etc.), ice confectionery (ice candy, ice cream, sorbet, etc.), frozen confectionery (jelly). , Pudding, water yokan, etc.), starch-based foods such as noodles, powdered foods and drinks, beverages (soup, coffee, teas, juice, carbonated beverages, cocoa, alcoholic beverages, jelly drinks, etc.), bakery foods (cookies) , Biscuits, breads, pies, cakes, etc.), oil and fat foods (margarine, shortening, fat spreads, etc.), dairy products (milk, yogurt, whey drinks, lactic acid bacteria beverages, butter, cream, cheese, etc.) .

The said food-drinks composition of this invention can be manufactured by a conventional method, respectively, The lipase inhibitor containing 1-mangostin by selecting suitably the following component in the range which does not impair the objective of this invention as needed. Can be blended together. For example, sugar sweeteners (monosaccharides such as fructose, glucose, tagatose and arabinose, oligosaccharides such as lactose, oligosaccharides, maltose and trehalose, powdered starch syrup, dextrin, sugar alcohol etc.), high sweetness sweeteners (sucralose, Acesulfame K, stevia, etc.), polysaccharides such as starch, fats and oils, dairy products, stabilizers, emulsifiers, fragrances (vanillin, linalool, natural fragrances, etc.), pigments, colorants, acidulants, flavor ingredients (eggs, coffee, Tea, cocoa, fruit pulp, yogurt, alcoholic beverages, etc.), flavoring (raspberry flavor, apple flavor, coffee flavor, etc.), anti-wetting agent, electrolyte, antioxidant, preservative, wetting agent, protein, amino acid, peptide , Dietary fiber, organic acids (citric acid, malic acid, fumaric acid, malonic acid, succinic acid, tartaric acid, lactic acid, etc.), vitamins (L-Asco Binic acid, dl-α-tocopherol, vitamin B1, vitamin B2, niacin, pantothenic acid, vitamin B6, vitamin B12, folic acid, biotin, inositol, etc., mineral (zinc, iron, calcium, magnesium, chromium, selenium, potassium, Sodium, etc.), glucosamine, yeast, eggshell membrane, lycopene, astaxanthin, other carotenoids, silk, chondroitin, ceramide, placenta extract, shark fin extract, deep-sea cocoon extract, squalene, γ-aminobutyric acid, casein decapeptide, raw chestnut skin extract , Chestnut leaf extract, chestnut potato extract, chestnut pulp extract, chestnut bark extract, cabbage ferment extract, rose petal extract, grape leaf extract, grape seed extract, apple polyphenol, chamomile extract, Lychee seed extract, Gotsukola extract Moon peach leaf extract, lotus germ extract, star fruit leaf extract, mulberry leaf extract, guava tea extract, red wine, green tea, black tea, oolong tea, coffee, cocoa, chocolate, black sesame, beans, soy milk, nuts, mushrooms, Green-yellow vegetables, egg-derived raw materials such as iodine eggs, catechins, other polyphenols, raspberry ketone, low molecular weight alginic acid, psyllium seed coat, ginkgo biloba extract, pine bark extract, nattokinase, plant sterol, diacylglycerol, chitosan, Examples include hyaluronic acid, methylsulfonylmethane, kojic acid, ellagic acid, arbutin, lucinol, magnolignan, magnesium phosphate L-ascorbate, CoQ10, and α-lipoic acid.

The dosage form of the pharmaceutical composition of the present invention is not particularly limited, and it may be an oral administration formulation or a parenteral administration formulation. Specifically, aerosols, liquids, extracts, elixirs, capsules (hard capsules, soft capsules, microcapsules), granules, pills, ophthalmic ointments, transdermal preparations, suspensions, emulsions, seats Preparation (vaginal preparation), powder, spirits, tablets (plain tablet, coated tablet, special tablet), syrup, soaking agent, decoction, injection (water-soluble injection, water-insoluble injection), patch, tincture Preparations, eye drops, lozenges, ointments, poultices, fragrances,
Examples include liniments, limonades, flow extracts, and lotions. These preparations can be produced by a conventional method in the pharmaceutical technical field, for example, by the method described in the Japanese Pharmacopoeia. These preparations can be safely administered to mammals including humans.

Since the lipase inhibitor containing 1-isomangosteen of the present invention is a compound having low toxicity to living organisms, it can be used as it is or in combination with a pharmacologically acceptable non-toxic and inert carrier. Can be applied as a pharmaceutical composition (including animal drugs) to mammals (eg, mice, rats, rabbits, dogs, cats, cows, horses, pigs, monkeys, etc.), fish, and the like.

The pharmacologically acceptable carrier is not particularly limited, and various carrier substances known as pharmaceutical materials can be used. The carrier substance is not particularly limited, and examples of solid carriers include excipients, lubricants, binders, and disintegrants. In addition to the carrier substance, formulation additives such as preservatives, colorants, natural pigments, sweeteners and the like can be used as necessary. These substances include lactose, hydroxypropylmethylcellulose, hydroxypropylcellulose, low-substituted hydroxypropylcellulose, ethylcellulose, corn starch, crystalline cellulose, carmellose calcium, anhydrous silicic acid, synthetic aluminum silicate, magnesium stearate, talc, etc. Although it can illustrate concretely, it is not restricted to these.

The carrier substance in the liquid preparation is not particularly limited, and examples thereof include a solvent, a solubilizing agent, a suspending agent, a tonicity agent, a buffering agent, and the like. Furthermore, formulation additives such as preservatives, colorants, water-insoluble lake pigments, sweeteners and the like can be used as necessary. These substances include isotonic agents such as mannitol, sodium chloride, glucose, sorbitol, glycerol, xylitol, fructose, maltose, mannose, stabilizers such as sodium sulfite, preservatives such as benzyl alcohol and methyl parahydroxybenzoate. In addition to the above, a solubilizer, a soothing agent, a pH adjuster and the like can be specifically exemplified, but are not limited thereto.

Hereinafter, the present invention will be specifically described by illustrating examples and comparative examples. In addition, this invention is not limited at all by these Examples.

-Preparation of 1-isomangosteen A fresh mangosteen fruit was cut open, the entire contents were taken out, and the mangosteen outer skin after the contents were removed was cut into small pieces and dried at 25 ° C. After putting 20 g of dried skin into a beaker, 100 ml of 50% aqueous ethanol solution was added and stirred for 48 hours to obtain a solvent extract. Ethanol was distilled off under reduced pressure as necessary, and the extract was freeze-dried to obtain a dried product. 1 g of the dried product was dissolved in 75% acetonitrile. The lysate was separated and purified at 25 ° C. by liquid high performance chromatography using a column YMC-Pack C8 (SH-243-5, manufactured by YMC Co., Ltd.) and a fluid phase (75% acetonitrile), and 1-isomangosteen Got.

Examples 1 to 3 and Comparative Example 1
The 1-isomangostin prepared above was prepared with 0.1 mol / L Tris-hydrochloric acid buffer (pH 8.0) so as to obtain the concentration of each Example shown in Table 1 and Table 2, and a sample solution was obtained. . The sample solution of Comparative Example 1 was a buffer solution only.

Test Example 1 Measurement of lipase inhibitory action derived from porcine pancreas The lipase activity was calculated by measuring the amount of oleic acid released from triolein. Triolein (SIGMA Chemical Co. (US)) 80 mg, lecithin 10 mg (Wako Pure Chemical Industries, Ltd.), bile acid (SIGMA Chemical Co. (US)) 5 mg, 9 ml of 0.1 mol (M) TES buffer A uniform suspension was obtained by sonication in a liquid (pH 7.0) for 10 minutes, and this was used as a substrate liquid. Add 0.1 ml of porcine pancreas-derived lipase solution (SIGMA Chemical Co. (US)) and 0.1 ml of the test substance to 0.1 ml of the substrate solution and react at 37 ° C. for 30 minutes to release. The measured fatty acid was quantitatively measured by a copper reagent method. For the lipase activity value derived from porcine pancreas, the lipase activity value and standard deviation of each sample were calculated with the value of no sample (control) added as 100%. The lower the activity value, the stronger the lipase inhibitory action.

As shown in Table 1, 1-isomangostin inhibited pancreatic lipase activity in a concentration-dependent manner.

Test Example 2 Measurement of Acne Bacterial Lipase Inhibition The preparation of Acne bacteria-derived lipase was carried out according to the method of Pablo et al. (Gary Pablo et al., “CHARACTERISTICS OF THE EXTRACE
The lipase was purified according to EPIDERMIS FREE, Journal of Investigative Dermatology, The Williams & Wilkins Co., August 1974, Vol. 63 No. 2 p.231-238). As an operation, inoculate 1 platinum ear of Propionibacterium acnes isolated from human face in brain heart infusion broth medium, and culture anaerobically at 37 ° C for 7 days. Thereafter, the culture solution was centrifuged at 3000 rpm for 20 minutes at 4 ° C. Add 500 ml of −20 ° C. and 95% ethanol to 500 ml of the supernatant, stir with a stirrer at −10 ° C. for 1 hour, centrifuge at 4 ° C. and 1000 rpm for 20 minutes, freeze-dry the precipitate, and test as crude lipase It was used for. For measurement of lipase activity inhibition, 4-methylumbelliferone oleate (4-MUO) was used as a substrate. After adding 0.1 ml of 1-isomangostin to 0.1 ml of 4-MUO solution and mixing, 0.05 ml of a lipase solution derived from acne is added and allowed to react for 30 minutes. 1M citrate buffer (pH 4.2) was added to stop the reaction. The fluorescence (excitation wavelength: 355 nm, fluorescence wavelength: 460 nm) of 4-methylumbelliferone generated by the reaction was measured with a fluorescence microplate reader. As the activity value, the activity value of each sample was calculated with the value of no sample being added as 100%.

As shown in Table 2, 1-isomangostin inhibited lipase activity derived from Acne bacteria in a concentration-dependent manner.

From the above results, 1-isomangosteen has an excellent lipase inhibitory effect, and can be expected to delay intestinal absorption of lipids and inhibit the activity of skin resident bacteria such as acne. Thus, it is considered that the composition containing 1-isomangosteen suppresses the occurrence of acne and fat accumulation, and has preventive, ameliorating, and therapeutic effects for various diseases.

Below, the Example of the food-drinks composition containing the lipase inhibitor of this invention, cosmetics, and a pharmaceutical composition is shown. The composition is expressed in mass%.

Example 4 Chewing gum A chewing gum having the following composition was prepared by a conventional method.
1-Isomangosteen 2.0
Gum base 20.0
Reduced water candy 4.0
Apple flavoring 0.5
Maltitol TO 100

When a monitor suffering from weight gain took the above chewing gum, which was 1500 mg per tablet, once a day, three times a day, for three months, the increase in body weight was suppressed compared to before.

Example 5 Tablet confectionery A tablet confection having the following composition was prepared by a conventional method.
1-Isomangostin 5.0
Sucrose fatty acid ester 4.0
Raspberry flavor 0.1
Sorbitol TO 100

When a monitor suffering from an increase in body weight took 1 tablet of 2000 mg of the above tablet confection once a day, 3 times a day for 3 months, the increase in body weight was suppressed compared to before.

Example 6 Milky skin cosmetic 1-Isomangostin 0.1
Liquid paraffin 8.0
Stearyl alcohol 3.0
Carrageenan 0.2
Paraben 0.1
Polyoxyethylene (60) hydrogenated castor oil 0.1
Perfume (Japanese cypress oil) Appropriate amount of purified water TO 100

Example 7 Square plug removal sheet,
1-Isomangosteen 1.0
Polyacrylic acid 27.6
Acrylic acid / methacrylic acid copolymer 35.0
Polyvinyl alcohol 5.4
Polyvinylpyrrolidone 18.8
Glycerin 4.2
Titanium oxide 3.0
Silica 5.0
Methyl paraoxybenzoate 0.2
Ethanol 2.4
Fragrance 0.5
Purified water TO 100

Example 8 Pap 1-Isomangostin 1.0
Glycerin 25.0
Sodium polyacrylate 3.0
Polyvinyl alcohol 2.0
Gelatin 1.5
Sodium carboxymethylcellulose 0.5
Ethanol 1.0
Edetate disodium 0.1
Methylparaben 0.05
Propylparaben 0.05
Purified water TO 100

Example 9 Gel Acne Cosmetic 1-Isomangostin 2.0
Polyethylene glycol 4000 30.0
Polyvinyl alcohol 15.0
Glycerin 15.0
3-BG 10.0
Carboxyvinyl polymer 2.0
Cationized cellulose 0.2
Alcohol 10.0
Paraben 0.1
Fragrance Slightly purified water TO 100

Example 10 Creamy Nutritional Cream 1-Isomangosteen 4.0
Stearic acid 2.5
Stearyl alcohol 8.0
Squalane 5.0
Octyldodecanol 7.0
Glyceryl monostearate 4.0
Preservative Appropriate amount of perfume Appropriate amount of glycerin 6.0
Sorbitol 13.0
Potassium lactate 0.5
Purified water TO 100

Example 11 Stick Lip Treatment 1-Isomangosteen 1.0
Candelilla Row 12.0
Solid paraffin 8.0
Beeswax 5.0
Carnavalo 2.0
Lanolin 14.0
Mannit 15.0
Isopropyl myristate 10.0
Perfume Appropriate amount Antioxidant Appropriate amount Castor oil TO 100

Example 12 Emulsion skin cosmetic 1-Isomangostin 5.0
Stearic acid 2.0
Cetanol 2.0
Vaseline 2.0
Lanolin alcohol 1.5
Liquid paraffin 9.0
Squalane 3.5
Sorbitol 10.0
Triethanolamine 1.0
Propylene glycol 5.0
Preservative appropriate amount perfume appropriate amount purified water TO 100

Example 13 Pasty nourishing cream 1-isomangosteen 2.0
Stearic acid 2.0
Stearyl alcohol 8.0
Reduced lanolin 2.0
Squalane 8.0
Octyldodecanol 6.0
Preservatives Appropriate perfumes Appropriate propylene glycol 5.0
Glycerin 3.0
Lactic acid 0.3
Sodium citrate 0.5
Purified water TO 100

Example 14 Liquid Slimming Massage Soap 1-Isomangosteen 5.0
Lauric acid 10.0
Potassium hydroxide 2.8
Coconut oil fatty acid potassium 30.0
Edetate disodium 0.15
Methylcellulose 0.5
Aminopropyl betaine laurate solution 2.0
Glycerin 8.0
Perfume proper amount purified water TO 100

Example 15 Massage Gel 1-Isomangosteen 10.0
Caffeine 0.1
Carboxyvinyl polymer 0.5
Polyvinyl alcohol 3.0
Glycerin 35.0
Polyethylene glycol 400 45.0
Ethanol appropriate amount Preservative appropriate amount purified water TO 100

Example 16 Bathing Solid Tablet Sodium Bicarbonate 40.0
Succinic acid 10.0
Anhydrous citric acid 10.0
Purified white sugar 10.0
Lactose 10.0
Dextrin 5.0
Polyethylene 6000 5.0
Sodium carboxymethylcellulose 4.0
Sodium tetradecenoate 4.0
L-cysteine hydrochloride 0.2
Edetic acid disodium 0.2
Fragrance 0.2
Titanium oxide 0.2
Kaolin 0.2
Sodium lauryl sulfate 0.1
Sodium oxalate 0.1
Natural vitamin E 0.1
1-Isomangostin 0.5
Colorant Slightly anhydrous sodium sulfate TO 100

Example 17 Powdered Sodium Bicarbonate for Bath 40.0
Silicic anhydride 0.5
Sodium chloride 10.0
1-Isomangostin 0.5
Fragrance 1.0
Colorant Slightly anhydrous sodium sulfate TO 100

Example 18 Granule powder detergent granular saccharide * 10.0
1-Isomangosteen 1.0
Sodium bicarbonate 30.0
Dry sodium sulfate 30.0
Sodium lauryl sulfate 1.5
PEG6000P 5.0
PVP 0.1
Perfume appropriate amount dextrin TO 100
* Granular saccharides manufactured by Meiji Seika containing white sugar, lactose and dextrin and encapsulating high-pressure gas were used.

Example 19 Powder Pack 1-Isomangostin 3.5
Talc 25.0
Propylene glycol 6.0
KCl 1.0
Fragrance 0.5
Kaolin TO 100

Example 20 Acne Gel (External Pharmaceutical Composition)
1-Isomangosteen 10.0
Caffeine 0.1
Carboxyvinyl polymer 0.5
Polyvinyl alcohol 3.0
Glycerin 35.0
Polyethylene glycol 400 45.0
Ethanol appropriate amount Preservative appropriate amount purified water TO 100

Each cosmetic of Examples 6 to 20 was prepared by a conventional method. All of them were not irritating to the skin, were excellent in the lipase prevention effect, the acne improvement effect, and the feeling in use was also excellent.

Example 20 Tablet (Pharmaceutical Composition)
1-Isomangosteen 10.0
Vitamin C 20.0
Vitamin B1 0.5
Vitamin B2 0.5
Lactose 40.0
Dextrin 23.0
Synthetic aluminum silicate 5.0
Magnesium stearate 1.0

(operation)
Each of the above components was mixed, and the mixture was tableted into 500 mg per tablet with a tableting machine to obtain a tablet containing 1 mg of 1-isomangostin in one tablet.

When a patient with hyperlipidemia took the above tablet once a day, 3 times a day for 3 months, the blood fat level decreased.

The lipase inhibitor of the present invention suppresses the occurrence of acne by acting on skin resident bacteria such as acne, and also suppresses digestion and absorption of fat in the body by acting as a lipolysis inhibitor. In addition, fat accumulation in the body can be prevented, treated, and improved. As a result, cosmetics, food and beverage compositions and pharmaceutical compositions containing the lipase inhibitor of the present invention can be used for the prevention, treatment and improvement of acne, obesity, hyperlipidemia, and fatty liver. is there.

Claims (4)

A lipase inhibitor comprising 1-isomangosteen as an active ingredient. A cosmetic comprising the lipase inhibitor according to claim 1. The food-drinks composition containing the lipase inhibitor of Claim 1 A pharmaceutical composition comprising the lipase inhibitor according to claim 1.