JP2012100639A - Method of washing, application and antibacterial treatment using enzyme treatment solution - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a method of washing, application and antibacterial treatment solution using an enzyme treatment solution which can maintain antibacterial action for a long time by effectively separating and removing dirt (residue) forming on the surface of the subject of treatment such as a food manufacturing facility or a cooking facility.SOLUTION: An enzyme treatment solution formed by adding and mixing in 100 pts.wt. of cold or hot water (80°C or less) an enzyme treatment agent comprising 0.2-1.2 pts.wt. of papain and 0.1-1.4 pts.wt. of pancreatin as enzymes and 0.2-2.4 pts.wt. of adenosine triphosphate disodium salt, 0.2-1.2 pts.wt. of a Bacillus protease, and 0.2-1.0 pt.wt. of L-glutamic acid as auxiliary agents is used for the washing of or application to the subject of treatment for an antibacterial treatment.

Description

  The present invention relates to a cleaning, application and antibacterial treatment method using an enzyme treatment liquid, and more particularly to various treatment objects for coping with problems such as food hygiene, food allergies, and hospital infections in medical settings. The present invention relates to a cleaning, application and antibacterial treatment method using an enzyme treatment solution that can effectively achieve antibacterial treatment by washing or application.

  Conventionally, from the viewpoint of food hygiene, various types of foods are caused by problems such as secondary contamination due to residues after washing, problems such as nosocomial infections in the medical field, especially health damage due to food allergies, and lack of allergen indications. , Voluntary collection of food, etc. has occurred frequently.

  Therefore, for the purpose of monitoring food hygiene, by utilizing commercially available wiping kits such as “ATP wiping inspection” and “protein wiping inspection”, it is possible to connect to effective allergen countermeasures at the production site and cooking of foods, etc. It helps to build food allergy countermeasures in the field.

  However, at present, the wiping inspection is an inspection for voluntary management, and it is fundamental to determine and operate all of the inspection methods, inspection frequency, setting method of cleanliness management standard values, etc. at the user's responsibility. ing. Certainly, cleanliness is a very on-site operation, and the contents differ from one office to another, and it is clear that the standard method is difficult to present uniformly.

  In the conventional inspection method, inspections taken at the office are carried to the laboratory and inspected, but the inspection results are transmitted and displayed at the next inspection at the office, and receive appropriate guidance. Was common. Therefore, for business establishments that receive guidance, for example, they receive guidance on work one month or more before, so there is a problem that it takes a lot of time for improvement.

  Today, testers used for wiping inspection tend to be smaller and inspection costs tend to be reduced, so inspection results can be obtained immediately at the inspection site, so a cleanliness reference value that is the basis for pass / fail judgment is set in advance. Then, specific work improvement can be instructed with this reference value as a target, and rapid and efficient improvement can be expected. In other words, the specific setting of the cleanliness standard value contributes to the safety and security of food promoted by the Ministry of Agriculture, Forestry and Fisheries, and the importance of the cleaning process in terms of establishing a traceability system for foods, etc. It is possible to increase.

  In addition, as a preventive measure against food poisoning, allergen control has not yet been established compared to microbial control implemented at food production sites and cooking sites, so more warnings and unnecessary raw materials are needed. There is a tendency to avoid risks due to the addition.

  Therefore, it is useful for the establishment of food allergy countermeasures at food production sites and cooking sites, and from the viewpoint of food hygiene monitoring, secondary contamination problems caused by various types of washed residues, nosocomial infections at medical sites, etc. How to make the cleanliness of objects close to zero is a future issue. The concept of soiling for the various types of cleaning described above is not only the cause of the germs, but also the total including food residues, etc. Rather than relying on it, it is important to be able to instruct the various cleanings to be performed properly.

  From this point of view, chemical cleaning methods and physical cleaning methods are known as conventional cleaning methods, and cleaning methods using enzymes are also known (see Patent Documents 1 and 2). That is, it is known that in order to clean up to details, an enzyme cleaning method is required in addition to the chemical or physical cleaning method.

  As a chemical cleaning method, a method of using a cleaning solution composed of an alkaline aqueous solution, an alkaline cleaning agent or the like, for example, a cleaning solution of a sodium dodecyl sulfate / sodium hydroxide cleaning solution or the like is common. However, this type of cleaning solution has a problem in that a virus removal membrane made of a hydrophilic polymer is affected by an alkali or a surfactant and the membrane is altered.

  Physical cleaning methods include a method of forcibly scraping adhered substances with a sponge ball or high-pressure water flow, etc., liquid backwashing method, gas backwashing method, pressurizing operation method, bubbling method, ultrasonic method, electrophoresis Various methods such as a method have been proposed. However, the present situation is that it is not always possible to achieve satisfactory cleaning with only these cleanings, and there is a problem that it is expensive and requires a special apparatus.

  As a cleaning method using an enzyme, a predetermined amount of water or hot water (80 ° C. or less) put in a required water tank is immersed in a treatment solution in which 1% or more of an enzyme cleaner is added and mixed for about 15 minutes. This is a method for peeling and removing bacteria or residues.

JP 2006-055784 A JP 2006-116525 A

  As a result of examining the separation (removal) of dirt (residues) generated on the surface of a processing object such as a food manufacturing facility or a cooking facility, the present inventor found that the surface of the processing object such as a food manufacturing facility or a cooking facility Considering the separation and removal of the generated dirt (residue), when the dirt (residue) is derived from a plant, pay attention to components such as lignin to which fiber cells are adhered; Focusing on these components, it was confirmed that it is important to selectively remove these components and to separate them without denaturing them.

  As a result, papain and pancreatin are used as enzymes, and an enzyme treatment agent using adenosine triphosphate disodium salt (ATP), Bacillus protease, and L-glutamic acid in combination as an auxiliary agent is obtained. An enzyme treatment solution is prepared by appropriately adding and mixing the agent with an appropriate amount of water or hot water of 80 ° C. or less, and using this enzyme treatment solution, the target object having the required dirt (residue) is washed. As a result, it was found that components such as lignin can be separated and removed from plant-based materials, and components such as fat can be effectively separated and removed from animal-based materials.

  Accordingly, an object of the present invention is to wash, apply and antibacterial treatment using an enzyme treatment solution that can effectively separate and remove stains (residues) generated in food production facilities, cooking facilities, etc., and can maintain antibacterial action for a long time. It is to provide a method.

In order to achieve the above object, the cleaning, coating and antibacterial treatment method using the enzyme treatment solution according to the present invention is performed on 100 weight water or hot water (80 ° C. or lower).
As an enzyme
Papain 0.2-1.2 parts by weight Pancreatine 0.1-1.4 parts by weight As an auxiliary agent,
Adenosine triphosphate disodium salt 0.2 to 2.4 parts by weight Bacillus protease 0.2 to 1.2 parts by weight L-glutamic acid 0.2 to 1.0 parts by weight An enzyme treatment agent was added and mixed. The antibacterial treatment is performed by washing or applying to the object to be treated using the enzyme treatment solution prepared in the above.

  Further, in the present invention, the enzyme treatment liquid can be used to perform antibacterial treatment by washing or applying the food production machine tool and the food cooking machine tool as treatment objects to their surfaces.

  Furthermore, in the present invention, antibacterial treatment can be performed by using the enzyme treatment solution and washing or coating fruits and vegetables on the outer surface as treatment objects.

  Furthermore, in the present invention, the above-mentioned enzyme treatment solution can be used to perform antibacterial treatment by washing or applying humans and animals as objects to be treated for hand washing, face washing or hair washing, respectively.

  According to the cleaning, application and antibacterial treatment method using the enzyme treatment liquid according to the present invention, the treatment is performed by using the enzyme treatment liquid for a treatment object such as a food production machine tool or a food cooking machine tool. The cleaning of the object and the antibacterial treatment can be easily performed by a normal cleaning or coating method, and the operation of the enzyme treatment liquid used is generally used for the cleaning of the object to be processed and the retention of the antibacterial action. Can be held for a relatively longer period of time.

  In addition, according to the cleaning, coating and antibacterial treatment method using the enzyme treatment liquid of the present invention, the enzyme treatment liquid is used for fruits and vegetables, so that the outer surfaces of the fruits and vegetables are removed. The fruit can be easily washed by a normal washing or coating method, and the outer surface of the fruits and vegetables can be washed and antibacterial treated, and the fruits can be treated by the action of the enzyme treatment solution used. And the freshness of vegetables can be kept relatively longer than usual.

  Furthermore, according to the cleaning, application and antibacterial treatment method using the enzyme treatment liquid of the present invention, the enzyme treatment liquid is used for hand washing, face washing or hair washing of humans and animals, and by a normal washing or coating method. It can be easily performed, and it can be washed and antibacterial treated for the hands, faces and hairs of humans and animals, and has a cosmetic effect on the surfaces of the humans and animals hands, faces and hairs. It can be remarkably enhanced by the action of the enzyme treatment solution used.

  And according to the present invention, it is possible to quantify the points that lead to safety and security for foods by the actions and effects obtained with respect to the above-mentioned foods for various processing objects that perform washing, application and antibacterial treatment. In addition, it can contribute to the construction of countermeasures such as secondary contamination accidents of food, food allergies, hospital-acquired infections at medical sites.

  Next, embodiments of cleaning, coating and antibacterial treatment methods using the enzyme treatment solution according to the present invention will be described in detail below.

In this example, as an enzyme treatment agent consisting of an enzyme and an auxiliary agent in 100 weight water or hot water (80 ° C. or less),
Papain 1.0 part by weight Pancreatin 0.5 part by weight Adenosine triphosphate disodium salt 1.0 part by weight Bacillus protease 0.7 part by weight L-glutamic acid 0.7 part by weight An enzyme treatment solution used for cleaning, coating and antibacterial treatment according to the invention was prepared.

  The enzyme treatment solution thus prepared was used by a usual method of washing or coating the object to be treated such as food production machinery / equipment and food cooking machinery / equipment producing stains (residues). As a result, the treatment object can be cleaned and antibacterial treated, and the treatment of the treatment object and the retention of the antibacterial action are compared with those of a normal case due to the action of the enzyme treatment liquid used. It was confirmed that it can be maintained for a long period of time. Further, depending on the use of the treatment object, after pretreatment of the treatment object using an existing cleaning agent, the treatment object is immersed in the treatment tank in which the enzyme treatment liquid according to the present invention is prepared for about 15 minutes. Of course, it is possible to effectively obtain the above-described cleaning effect and retention of antibacterial action by washing with water.

  When obtaining an enzyme treatment solution for use in the cleaning, coating and antibacterial treatment methods according to the present invention, contaminants such as lignin, polysaccharides and fats and oils present in plants and animals are removed efficiently and nearly completely. It is important to be able to do it. Therefore, in the present invention, from the viewpoint that it is desirable to use an auxiliary agent that promotes the action of the enzyme as the enzyme treatment agent, papain and pancreatin are used as the enzyme, and adenosine triphosphate disodium as the auxiliary agent. By using a salt (ATP), Bacillus protease, and L-glutamic acid in combination, it is possible to remarkably increase the efficiency of the cleaning treatment of the enzyme treatment solution to be produced.

  That is, in the cleaning treatment using the enzyme treatment liquid according to the present invention, by using papain and pancreatin as enzymes, food production machinery and food cooking machines mediated by the above lignin, polysaccharides, fats and oils, etc. It can be separated and removed from a processing object such as an instrument. In addition, by using adenosine triphosphate disodium salt (ATP), a Bacillus protease, and L-glutamic acid as an auxiliary agent, the function of the enzyme is promoted, and the impurities separated and removed from the object to be treated are removed. Objects can be discharged out of the tissue reaction system.

  In preparing the enzyme treatment solution used in the present invention, the amount of the enzyme used for water or hot water (80 ° C. or less) is such that impurities such as lignin, polysaccharides, fats and oils are efficiently separated and removed from the treatment object. Thus, with respect to 100 parts by weight of water or hot water (80 ° C. or lower), papain is preferably 0.2 to 1.2 parts by weight, and pancreatin is preferably 0.1 to 1.4 parts by weight. If the amount of at least one kind used is less than this, the balance of separation and removal of each of the foreign substances will be lost, and the amount of residual dust corresponding to each enzyme will increase, and even if the amount used increases. This is not preferable because the effect of separating and removing the respective impurities is not improved, and is decomposed.

  In the present invention, other enzymes can be used in combination with papain and pancreatin as the enzyme. In particular, when adenosine triphosphate disodium salt (ATP) is used in the range of 0.2 to 2.4 parts by weight, an effect of accelerating the action of promoting separation and removal of impurities by papain and pancreatin can be obtained. . Moreover, the usage-amount of papain can be reduced by using together Bacillus microbe protease in 0.2-1.2 weight part. And by using together L-glutamic acid in the range of 0.2-1.0 weight part, the reaction which a contaminant decomposes | disassembles from the component (molecule | numerator) of a soil (residue) with an enzyme can be accelerated.

  Furthermore, the pH value of the enzyme treatment solution used in the present invention is preferably set to pH 8 to 10 so that all the enzymes used for the washing treatment of the object to be treated work sufficiently effectively. In this case, sodium bicarbonate (sodium bicarbonate) is preferred as the pH adjuster because it is easy to adjust.

In this example, as an enzyme treatment agent consisting of an enzyme and an auxiliary agent in 100 weight water or hot water (80 ° C. or less),
Papain 0.6 parts by weight Pancreatin 0.4 parts by weight Adenosine triphosphate disodium salt 0.8 parts by weight Bacillus protease 0.6 parts by weight L-glutamic acid 0.8 parts by weight An enzyme treatment solution used for cleaning, coating and antibacterial treatment according to the invention was prepared.

  The enzyme-treated solution prepared in this manner is obtained by using grapes and pears as fruits and using pepper and grapes as vegetables and treating them with fruits and vegetables. The outer surface was used by a normal cleaning or coating method. As a result, the outer surfaces of the fruits and vegetables can be washed and antibacterial treated, and the freshness of the fruits and vegetables can be made higher than usual by the action of the enzyme treatment solution used. It was confirmed that it can be maintained for a relatively long time.

In this example, as an enzyme treatment agent consisting of an enzyme and an auxiliary agent in 100 weight water or hot water (80 ° C. or less),
Papain 1.2 parts by weight Pancreatin 0.8 parts by weight Adenosine triphosphate disodium salt 0.8 parts by weight Bacillus protease 0.4 parts by weight L-glutamic acid 0.4 parts by weight An enzyme treatment solution used for cleaning, coating and antibacterial treatment according to the invention was prepared.

  The enzyme treatment solution prepared in this manner is used for adult women as human subjects and livestock poodle dogs as animals, and by ordinary washing or application methods for hand washing, face washing or hair washing in each subject. did. As a result, washing and antibacterial treatment can be performed on the hands, faces and hairs of humans and animals, and the enzyme treatment used for the cosmetic effect on the surfaces of humans and animals, faces and hairs. It was confirmed that it was remarkably enhanced by the action of the liquid.

Claims (4)

To 100 weight water or hot water (80 ° C or less),
As an enzyme
Papain 0.2-1.2 parts by weight Pancreatine 0.1-1.4 parts by weight As an auxiliary agent,
Adenosine triphosphate disodium salt 0.2 to 2.4 parts by weight Bacillus protease 0.2 to 1.2 parts by weight L-glutamic acid 0.2 to 1.0 parts by weight An enzyme treatment agent was added and mixed. A cleaning, coating, and antibacterial treatment method using an enzyme treatment liquid characterized in that an antibacterial treatment is performed by washing or coating an object to be treated using the enzyme treatment liquid prepared in the above.   Washing, coating, and antibacterial treatment using the enzyme treatment solution according to claim 1 to wash or apply food production machinery and food cooking machinery to the surfaces thereof as treatment objects Antibacterial treatment method.   A cleaning, coating and antibacterial treatment method characterized in that the enzyme treatment solution according to claim 1 is used, and fruits and vegetables are treated as objects to be treated and washed or applied to perform antibacterial treatment. .   Washing, coating and antibacterial treatment using the enzyme treatment solution according to claim 1, wherein humans and animals are treated as objects to be treated and washed or applied for hand washing, face washing or hair washing. Method.