JP2012097077A - Anti-obesity agent or blood triglyceride depressant containing aqueous extract of schisandra chinensis - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a therapeutic agent which originates from a medical plant and can be safely given or taken over a long period for preventing or treating obesity, or a food composition having anti-obesity effect; and a therapeutic agent which originates from a medical plant and can be safely given or taken over a long period for preventing or treating hypertriglyceridemia, or a food composition having blood neutral fat (triglyceride) reducing effect.SOLUTION: A pharmaceutical/food composition containing an aqueous extract of Schisandra chinensis as an active ingredient is useful for preventing, improving, and/or treating obesity and hypertriglyceridemia, since the pharmaceutical/food composition exhibits excellent weight gain suppressing effect, body fat increase suppressing effect, and a blood neutral fat (triglyceride) reducing action and hardly causes side effects.

Description

  The present invention relates to an extract having anti-obesity activity and blood triglyceride lowering effect obtained from a medicinal plant. More specifically, a medicament for the prevention, amelioration, and / or treatment of obesity or hypertriglyceridemia, which contains, as an active ingredient, an aqueous extract from ginseng, a medicinal plant traditionally used in the Orient. The present invention relates to a composition or a food composition having an anti-obesity action or a blood triglyceride lowering action.

  Obesity is a disease induced by abnormal metabolism in which body fat is abnormally increased due to excessive energy being converted into fat and excessive accumulation in adipose tissue. The causes of obesity mainly include genetic causes and environmental causes (eating habits, lack of exercise, etc.). Besides these, obesity due to neuroendocrine causes, drug causes and the like are also known.

  In recent years, dietary habits centered on high-fat diets, weight gain due to lack of exercise, and the resulting obesity have become a risk of developing lifestyle-related diseases such as diabetes, hypertension, and hyperlipidemia. It is considered to be important to reduce the incidence of lifestyle-related diseases by suppressing weight gain. In addition, from the viewpoint of appearance and beauty, there is a trend that obesity is not preferable, which sometimes causes anorexia and bulimia and often requires medical care.

  Although it is widely recognized that it is important to improve dietary habits, such as improving dietary content and exercising enough to suppress weight gain, changing lifestyles is actually not possible It is not easy, and there are many cases that cannot be covered by that alone. Therefore, there is a strong demand for drugs and foods that suppress obesity and weight gain.

  Targets for treating obesity include appetite regulation, fat metabolism regulation, adipocyte differentiation regulation, fat absorption regulation, energy metabolism regulation and the like, and active research is being conducted. Anti-obesity drugs approved to date include sibutramine, a catecholamine / serotonin reuptake inhibitor, orlistat, an inhibitor of pancreatic lipase involved in lipid metabolism, and cannabinoid (CB1) receptors. Inhibitors such as rimonabant are known.

  However, side effects such as increased blood pressure, insomnia, dry mouth and dizziness have been reported for sibutramine, and side effects such as diarrhea, fatty stool and stool incontinence are known for orlistat, and emotional aspects for rimonabant Since side effects in (depressed state) are known, pharmacotherapy using the above anti-obesity drugs is limited to patients with severe obesity.

  On the other hand, plant extracts that have been used for a long time in folk remedies and traditional medicine (eg, Kampo medicine) have the advantage that they can be taken or ingested safely for a long period of time with little risk of side effects. Yes. For this reason, exploratory research on plant-derived medicines or food materials exhibiting an anti-obesity effect that can be used as pharmaceuticals and functional foods has been actively conducted in Japan and overseas.

  Ginseng (Schisandra chinensis) is a plant belonging to the genus Schisandraceae, which is obtained by drying its mature fruit. In Chinese medicine, the water or alcohol extract has been used for the treatment of antitussive, reduced energy, nourishing tonic, liver damage, and nervous breakdown (Non-patent Documents 1 and 2). However, there has been no report on whether or not the Gomicho extract is useful for prevention, improvement or treatment of obesity.

In recent years, it has become clear that the neutral fat (triglyceride) concentration in blood and obesity do not necessarily correlate. L. Nanni et al. Reported that the triglyceride level in the familial hyperlipidemia group was about twice that of the control group even though the BMI (Body Mass Index) value was equivalent. cage (non-Patent Document 3), M. Miller et al., about 80% of the blood triglyceride value 150 mg / dL or more subjects prior obese (BMI value of less than 25 kg / ^{m 2} or more 30 kg / ^{m 2)} and obesity Although the BMI value was 30 kg / m ² or more, 20.1% of the subjects reported that the BMI value was less than 25 kg / m ² (Non-patent Document 4). Furthermore, in a study of 162 Japanese subjects, H. Nakarai et al., In subjects with moderate BMI (21.5 kg / m ^{2 to} 27.0 kg / m ² ) values, It has been reported that no correlation was found between waist circumference and BMI value (Non-patent Document 5).
That is, obesity and blood triglyceride concentration do not necessarily correlate, indicating that there are cases where the blood triglyceride concentration is high even though the body weight is low. A high blood triglyceride concentration has been shown to be an important biomarker for coronary artery disease (CAD) such as angina pectoris and myocardial infarction (Non-patent Document 4).
Therefore, a drug capable of directly lowering blood triglycerides is also desired from the viewpoint of CAD prevention.

  However, as blood triglyceride lowering drugs, only fibrates such as clofibrate and clinofibrate, and nicotinic drugs such as tocopherol nicotinate and nicomol are mainly used. For fibrate drugs, a serious side effect called rhabdomyolysis has been reported, and it has been reported that the risk increases particularly when combined with a statin-type hypercholesterolemia drug (non-patented) Reference 6). On the other hand, for nicotinic acid drugs, side effects associated with a decrease in QOL, such as gastrointestinal symptoms such as facial flushing, heat sensation, diarrhea, and constipation, have been reported, and blood triglycerides can be reduced well without causing such side effects. The drug is not well known.

Kitagawa Isao et al., Biopharmaceuticals, 3rd edition, Yodogawa Shoten, p.256-257 Yamagata Prefectural Institute of Public Health, Eiken News, No.134, 2004.10.10, p.4 L. Nanni et al .; Atherosclerosis 213 206-211 (2010) M. Miller et al .; Circulation 123 2292-2333 (2011) H. Nkarai et al .; Metabolism Clinical and Experimental 60 823-829 (2011) Maeda et al .; Japanese Journal of Drugs 39 (8) 991-994 (2003)

The present invention has been made in view of the above circumstances, has an excellent anti-obesity action such as a weight gain inhibitory action, a body fat accumulation inhibitory action, etc., and is safe and has side effects even when taken or ingested over a long period of time. An object of the present invention is to provide a pharmaceutical or food containing a plant extract with less worries.
A further object of the present invention is to provide a pharmaceutical or food that can particularly reduce blood triglycerides well.

As a result of intensive studies to solve the above-mentioned problems, the present inventors have obtained a water-extracted extract that can be easily obtained from a herbal medicine "Gomi-ko" obtained by drying matured fruit of Korean ginseng, which has been used in traditional Chinese medicine or folk remedies. Has been found to have an excellent effect of suppressing weight gain and body fat accumulation, and is useful for the prevention, improvement and / or treatment of obesity, and has led to the completion of the present invention. That is, this invention relates to the food composition containing the anti-obesity agent which contains the ginseng waste water extract as an active ingredient, ie, the anti-obesity pharmaceutical composition, or the anti-obesity agent which consists of a ginseng water extract.
The present invention also relates to a method for preventing, ameliorating, and / or treating obesity using an extract of water extracted from Korean dung beetle.
Furthermore, the present invention also relates to a method for using an extract of water extract of Korean ginseng for producing a composition for preventing, ameliorating and / or treating obesity.

Furthermore, the present inventors have found that the above-mentioned extract of D. gondii water can particularly favorably reduce the neutral fat (triglyceride) in the blood. That is, the present invention relates to a blood triglyceride lowering agent and a food composition capable of lowering blood triglyceride. The present invention also relates to a method for preventing, ameliorating, and / or treating hypertriglyceridemia using an aqueous extract of Korean ginseng.
Furthermore, the present invention also relates to a method for using an aqueous extract of Korean dung bean for producing a composition for prevention, amelioration and / or treatment of hypertriglyceridemia.

That is, the present invention
[1] An anti-obesity agent containing an extract from Kojisen wastewater as an active ingredient,
[2] The antiobesity agent according to the above [1], which comprises an extract from Kojisen waste water,
[3] An aqueous solution obtained by adding water to a dried mature fruit of Korean ginger, extracting water at 60 ° C. to 100 ° C. for 0.5 to 8 hours, and removing the residue by filtration. An anti-obesity agent according to [1] or [2] above,
[4] Daturated water extract is an aqueous solution obtained by adding water to a dried dried fruit of Datura, extracting at 80 ° C. to 100 ° C. for 1 to 4 hours, and removing the residue by filtration, The antiobesity agent according to [3] above,
[5] The above-mentioned [4] is an aqueous solution obtained by adding water to an extract of matured fruit of Korean dung bean, adding water to the dried fruit, boiling and extracting at 100 ° C. for 1 hour, and removing the residue by filtration. An anti-obesity agent,
[6] The above-mentioned [1], wherein the extract of water extract of Korean ginseng is an aqueous solution obtained by adding water to a dried mature fruit of ginseng, cooling at room temperature for 8 to 12 hours, and removing the residue by filtration. Or the antiobesity agent according to [2],
[7] The anti-obesity agent according to any one of the above-mentioned [3] to [6], wherein the extract of D. chinensis water is a powder obtained by drying the aqueous solution,
[8] A weight gain inhibitor comprising the anti-obesity agent according to any one of [1] to [7] above,
[9] The weight gain inhibitor according to [8] above, wherein the weight gain is weight gain before reaching obesity,
[10] The weight gain inhibitor according to the above [8], wherein the weight gain is a weight gain of an obese patient,
[11] The body weight gain inhibitor according to any one of the above [8] to [10], wherein the content of the extract of the water extract of Korean ginseng is an effective amount for suppressing body weight gain,
[12] The body weight gain inhibitor according to [11] above, wherein the effective amount is 0.1 mg to 2000 mg / kg body weight / day as a dry weight,
[13] A body fat increase inhibitor comprising the anti-obesity agent according to any one of [1] to [7] above,
[14] The body fat increase inhibitor according to the above [13], wherein the increase in body fat is an increase in neutral fat,
[15] A blood triglyceride lowering agent containing an extract of Kojisen Kojishi as an active ingredient,
[16] The blood triglyceride lowering agent according to the above [15], which comprises an extract from Kytosei rubble,
[17] An aqueous extract obtained by adding water to a dried fruit of matured ginseng, extracting water at 60 ° C. to 100 ° C. for 0.5 to 8 hours, and removing the residue by filtration. A blood triglyceride lowering agent according to [15] or [16] above,
[18] The extract of Korean ginseng water is an aqueous solution obtained by adding water to a dried mature fruit of ginseng, extracting at 80 ° C. to 100 ° C. for 1 to 4 hours, and removing the residue by filtration, The blood triglyceride lowering agent according to the above [17],
[19] The above-mentioned [18] is an aqueous solution obtained by adding water to a dried matured fruit of Korean ginger, adding boiling water at 100 ° C. for 1 hour, and removing the residue by filtration. A blood triglyceride lowering agent according to claim 1,
[20] The above-mentioned [15], wherein the extract of water extract of datura is an aqueous solution obtained by adding water to a dried mature fruit of datura, cooling at room temperature for 8 to 12 hours, and removing the residue by filtration. Or the blood triglyceride lowering agent according to [16],
[21] The blood triglyceride lowering agent according to any one of the above [17] to [20], wherein the water extract of Korean ginseng extract is a powder obtained by drying the aqueous solution, and [22] the above [ 15] to [21], a therapeutic agent for hypertriglyceridemia, comprising the blood triglyceride lowering agent according to any one of
About.

  The present invention also relates to the prevention and improvement of obesity, characterized in that an animal, particularly a mammal, ingests or administers an effective amount of an extract of water extract of datura water that suppresses body weight gain and body fat accumulation. And / or treatment methods; and weight gain inhibitors, body fat accumulation inhibitors, obesity prevention and treatment agents, food compositions having anti-obesity action, pet foods, feeds or feeds (hereinafter also referred to as food compositions, etc.) And a method for using the extract of D. gondii water for the production of a food composition having an anti-obesity effect.

  Still further, the present invention relates to the prevention and improvement of hypertriglyceridemia characterized by ingesting or administering an effective amount of an extract of water extract of datura which reduces triglycerides in blood to animals, particularly mammals. And / or therapeutic method; and a preventive and therapeutic agent for hypertriglyceridemia, a food composition having a blood triglyceride-lowering action, and a method for using a water extract of Korean ginseng for producing the food composition and the like Also provide.

  An anti-obesity agent having a body weight gain inhibitory action, a body fat accumulation inhibitory action, etc., or a food composition having an anti-obesity action according to the present invention, which contains an extract of an extract of ginseng water as an active ingredient, prevents obesity, improves, and / Or can be used for therapeutic applications. In addition, since ginkgo biloba is a plant-derived component with dietary experience, it has few side effects and can be safely taken or ingested over a long period of time.

  Further, according to a preferred aspect of the present invention, for example, obesity can be prevented, improved, and / or treated by suppressing body weight gain and body fat accumulation without affecting palatability and food intake. Can be done without.

  Furthermore, the anti-obesity composition of the present invention can prevent, improve, and / or obesity by safely suppressing weight gain and body fat accumulation without inhibiting absorption of nutritional components necessary for maintaining health. Alternatively, treatment can be expected.

  Furthermore, the blood triglyceride-lowering agent of the present invention can satisfactorily lower blood triglycerides even in a state where blood triglycerides are elevated regardless of obesity and body fat accumulation. Therefore, the therapeutic agent for hypertriglyceridemia containing the blood triglyceride lowering agent of the present invention can satisfactorily prevent, ameliorate and / or treat hypertriglyceridemia, and particularly effectively reduce the risk of developing CAD. can do.

It is a graph which shows the body weight change of the rat which administered the high-fat diet containing an extract of Kyojimushi water. It is a graph which shows the comparison of the food intake by the rat about a normal diet, a high-fat diet, or a high-fat diet containing an extract of water extract of Korean ginseng. It is a graph which shows the result of the oral glucose tolerance test (OGTT) with respect to the rat which ingested the normal diet, the high fat diet, or the high fat diet containing the ginseng water extract extract for 10 weeks. It is a graph which shows the comparison of the total cholesterol amount of the rat which ingested the normal diet, the high fat diet, or the high fat diet containing the ginseng extract extract for 10 weeks. It is a graph which shows the comparison of the amount of triglycerides (TG amount) in the plasma of the rat which ingested the normal diet, the high fat diet, or the high fat diet containing the ginseng extract extract for 10 weeks. It is a graph which shows the comparison of the tissue weight of the visceral fat of the rat which ingested the normal diet, the high fat diet, or the high fat diet containing the ginseng extract extract for 10 weeks. It is a graph which shows the comparison of the amount of blood leptin of the rat which ingested the normal diet, the high-fat diet, or the high-fat diet containing the ginseng extract extract for 10 weeks. It is the microscope picture which stained hematoxylin the fat tissue of the dissected rat after ingesting a normal diet, a high fat diet, or the high fat diet containing a ginseng extract extract for 10 weeks. It is a graph which shows the change of the intake of the db / db mouse | mouth which administered the normal meal or the meal mixed with the extract of Kojimoshi water extract. It is a graph which shows the blood triglyceride (TG) value in the db / db mouse | mouth which administered the normal meal or the meal mixed with the extract of an aqueous extract of datura. It is a graph which shows the change of the blood triglyceride (triglyceride) density | concentration after oral administration of olive oil in the rat which administered the ginseng extract, and the rat of a control group.

  Hereinafter, the present invention will be described in detail.

1. Daturated water extract Extract used in the present invention is a plant belonging to the genus Brassicaceae, and as a Daturated water extract, a herbal medicine "Gomi-ko" that is a dried mature fruit of Datura In the present invention, it is preferable to use a commercially available JP “Gomiko”). In addition, “Kadsura japonica”, a plant belonging to the genus Scisandraceae, can be used in the same manner as Korean dung beetle.

  In the extraction, for example, 1 to 50 times (preferably 2 to 20 times, more preferably 5 to 15 times, and particularly preferably 10 times) of water is used at 60 to 100 ° C. , (Preferably 80 to 100 ° C., more preferably 90 to 100 ° C., particularly preferably 100 ° C.) for 0.5 to 8 hours (preferably 1 to 4 hours, more preferably 1 to 2 hours, particularly preferably 1 hour) extracted (digestion method), removed herbal medicine by filtration, and concentrated to 1/2 to 1/20 amount (preferably 1/5 to 1/10 amount, more preferably 1/10 amount). The aqueous solution can be used as it is. In addition, water of 1 to 50 times (preferably 2 to 20 times, more preferably 5 to 15 times, particularly preferably 10 times) of water is used for the gomi, and the cold immersion method (at room temperature, 8 times). The extract obtained by extraction for ˜12 hours also has the same effect as the extract obtained by the digestion method. Further, an aqueous solution obtained by the digestion method or the cold immersion method may be used by concentrating and drying, spray-drying (powdering with a spray dryer), or freeze-drying to form a powder.

  In this specification, “room temperature” means the normal temperature of the atmosphere in the laboratory (65 to 80 ° F. (18.3 to 26.7 ° C.)).

2. Formulation and Food Composition Containing Daturated Water Extract Extract Daturated water extract extract has a body weight gain inhibitory activity, a body fat accumulation inhibitory activity, and a blood triglyceride lowering action. For example, as an agent for preventing, improving, and / or treating obesity or hypertriglyceridemia, or as a slimming agent (hereinafter sometimes abbreviated as “the preparation of the present invention”), and, for example, an effect of inhibiting weight gain. It can be used as a food composition having a body fat accumulation inhibitory effect, an obesity improving action, and a blood triglyceride lowering action (hereinafter sometimes abbreviated as “food composition of the present invention”).

  As a preferred method of administration or ingestion of the preparation or food composition of the present invention, administration during meals or after meals or ingestion is particularly preferred, but regular administration or ingestion forms may be used. In the case of the food composition of the present invention, there is no particular limitation on the administration or intake method.

Applicable animals include humans, dogs, cats, parakeets, cucumber butterflies, parrots, guinea pigs, rats, mice, pigs, sheep, goats, horses, cows, monkeys, rabbits, frogs, salamanders, goldfish, carp, carp, etc. Vertebrates are preferred, mammals are more preferred, and humans are particularly preferred. The target animal may be any animal that wants to avoid weight gain or body fat accumulation, that is, an animal that wants to prevent, ameliorate, or treat obesity and is genetically at risk of weight gain. It may be an animal suffering from a lifestyle-related disease such as diabetes, hypertension and / or hyperlipidemia.
In addition, it can be suitably used for animals that have hypertriglyceridemia or may develop hypertriglyceridemia and desire prevention of CAD.

The weight gain may be a weight gain before obesity or a weight gain of an obese patient. Obesity is defined as BMI (body mass index: body weight (kg) / [height (m)] ² ) of 30 or more according to WHO standards, but for Japanese, BMI is 25 or more. (According to the standards of the Japanese Society of Obesity) In the present invention, it is used synonymously.

  As described above, the extract of Korean ginseng water can be used as a body fat accumulation inhibitor or a food composition having an action of inhibiting body fat accumulation. Among them, a neutral fat mass increase inhibitor or a neutral fat can be used. It can be preferably used as a food composition having an inhibitory effect on the increase in amount, and in particular, it can be suitably used as an inhibitor for increasing the amount of triglyceride or a food composition having an inhibitory effect on the increase in triglyceride amount.

  Examples of tissues or organs in which accumulation of body fat is suppressed by the preparation or food composition of the present invention include, for example, liver, epididymal fat tissue, peritoneal fat tissue (perirenal, retroabdominal wall fat), flank fat Examples thereof include tissues or organs that are rich in adipocytes, such as tissues. Specifically, the preparation or food composition of the present invention can suppress the fat tissue weight and the fat cell hypertrophy.

  In addition, the water extract of Korean ginseng has an action of suppressing an increase in total cholesterol in the blood. More specifically, the extract of Korean ginseng water has an action of suppressing an increase in cholesterol other than HDL without affecting high-density lipoprotein (HDL) cholesterol in blood. Therefore, the extract of Korean ginseng water can be suitably used as a cholesterol increase inhibitor or a food composition having a cholesterol increase inhibitory action.

  Furthermore, the preparation or food composition of the present invention also has an action of suppressing the amount of leptin secreted, which increases secretion due to obesity and increases blood concentration.

  Also, as described above, the extract of Korean ginseng water has a good lowering effect on the increase in blood triglyceride seen regardless of obesity and body fat accumulation. Therefore, the preparation or food composition of the present invention containing the extract of Kyoritsugoshi water has a good preventive, ameliorating, and / or therapeutic effect against hypertriglyceridemia that occurs regardless of obesity and body fat accumulation. Have. As a result, the risk of developing CAD can be reduced well.

(1) Formulation of the present invention When using the extract from Kojisenshishi as an above-mentioned formulation, it can be carried out by a known method. Specifically, for example, it can be carried out as described below.

  The preparation of the present invention comprises tablets, pills, capsules (including hard capsules, soft capsules, microcapsules), powders, granules, fine granules, troches, liquids (syrups) with sugar coating or coating as necessary And the like, and emulsions and suspensions).

  As long as the effects of the present invention are not inhibited, a pharmacologically acceptable carrier or the like can be added to the preparation of the present invention. As the pharmacologically acceptable carrier, various organic or inorganic carrier substances commonly used as pharmaceutical materials are used. Excipients, binders, disintegrants, lubricants in solid preparations; solvents, dissolution in liquid preparations Auxiliaries, suspending agents, buffers, thickeners, emulsifiers and the like can be mentioned. In addition, formulation additives such as coloring agents, sweetening agents, and antioxidants can be used as necessary. Furthermore, you may coat the formulation of this invention.

  Examples of the excipient include lactose, sucrose, D-mannitol, D-sorbitol, starch, pregelatinized starch, dextrin, crystalline cellulose (for example, microcrystalline cellulose), low-substituted hydroxypropylcellulose, sodium carboxymethylcellulose, Examples include gum arabic, dextrin, pullulan, light anhydrous silicic acid, synthetic aluminum silicate, magnesium aluminate metasilicate, and the like. Examples of the binder include pregelatinized starch, sucrose, gelatin, macrogol, gum arabic, methylcellulose, carboxymethylcellulose, sodium carboxymethylcellulose, crystalline cellulose, sucrose, D-mannitol, trehalose, dextrin, pullulan, hydroxypropylcellulose ( HPC), hydroxypropylmethylcellulose (HPMC), polyvinylpyrrolidone (PVP), and the like. Examples of the disintegrant include lactose, sucrose, starch, carboxymethylcellulose, carboxymethylcellulose calcium, crosslinked polyvinylpyrrolidone, carmellose sodium, croscarmellose sodium, sodium carboxymethyl starch, light anhydrous silicic acid, low-substituted hydroxypropylcellulose, Examples include cation exchange resins, partially pregelatinized starch, and corn starch. Examples of the lubricant include stearic acid, magnesium stearate, calcium stearate, talc, waxes, colloidal silica, DL-leucine, sodium lauryl sulfate, magnesium lauryl sulfate, macrogol, and aerosil.

  Examples of the solvent include water for injection, physiological saline, Ringer's solution, alcohol, propylene glycol, polyethylene glycol, medium chain fatty acid triglyceride (MCT), vegetable oil (for example, safflower oil, sesame oil, corn oil, olive oil, cottonseed oil, soybean Lecithin and the like).

  Examples of solubilizers include polyethylene glycol, propylene glycol, D-mannitol, trehalose, benzyl benzoate, ethanol, trisaminomethane, cholesterol, triethanolamine, sodium carbonate, sodium citrate, sodium salicylate, sodium acetate, and the like. Can be mentioned.

  Examples of the suspending agent include surfactants such as stearyltriethanolamine, sodium lauryl sulfate, laurylaminopropionic acid, lecithin, benzalkonium chloride, benzethonium chloride, and glyceryl monostearate; for example, polyvinyl alcohol, polyvinylpyrrolidone, Hydrophilic polymers such as sodium carboxymethylcellulose, methylcellulose, hydroxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose; polysorbates, polyoxyethylene hydrogenated castor oil, and the like.

  Examples of the buffer include buffer solutions of phosphate, acetate, carbonate, citrate and the like.

  Examples of the thickener include natural gums and cellulose derivatives.

  Examples of the emulsifier include fatty acid esters (eg, sucrose fatty acid ester, glycerin fatty acid ester, sorbitan fatty acid ester, propylene glycol fatty acid ester), wax (eg, beeswax, rapeseed hydrogenated oil, safflower hydrogenated oil, palm Examples thereof include hydrogenated oil, sitosterol, stigmasterol, campesterol, brush casterol, cacao butter powder, carnauba wax, rice wax, molasses, paraffin, etc., lecithin (eg, egg yolk lecithin, soybean lecithin, etc.).

  Examples of the colorant include water-soluble edible tar dyes (eg, edible dyes such as edible red Nos. 2 and 3, edible yellows Nos. 4 and 5, edible blue Nos. 1 and 2), water-insoluble lake dyes (eg, And water-soluble edible tar pigments such as aluminum salts), natural pigments (eg, β-carotene, chlorophyll, bengara, etc.).

  Examples of the sweetening agent include sucrose, lactose, sodium saccharin, dipotassium glycyrrhizinate, aspartame, stevia and the like. Examples of the antioxidant include sulfite, ascorbic acid and alkali metal salts and alkaline earth metal salts thereof.

  Tablets, granules, fine granules and the like may be coated by a usual method using a coating substrate for the purpose of masking taste, improving light stability, improving appearance or enteric properties. Examples of the coating base include sugar coating base, water-soluble film coating substrate, enteric film coating substrate and the like.

  Examples of the sugar coating base include sucrose, and one or more kinds selected from talc, precipitated calcium carbonate, gelatin, gum arabic, pullulan, carnauba wax and the like may be used in combination.

  Examples of water-soluble film coating bases include cellulosic polymers such as hydroxypropylcellulose (HPC), hydroxypropylmethylcellulose (HPMC), ethylcellulose, hydroxyethylcellulose, and methylhydroxyethylcellulose; polyvinyl acetal diethylaminoacetate, aminoalkyl methacrylate copolymer Synthetic polymers such as E (Eudragit E (trade name), Rohm Pharma), polyvinylpyrrolidone, and polysaccharides such as pullulan.

  Examples of enteric film coating bases include cellulose polymers such as hydroxypropylmethylcellulose phthalate, hydroxypropylmethylcellulose acetate succinate, carboxymethylethylcellulose, and cellulose acetate phthalate; methacrylic acid copolymer L (Eudragit L (trade name)) Acrylic polymers such as Rohm Pharma), methacrylic acid copolymer LD (Eudragit L-30D55 (trade name) Rohm Pharma), methacrylic acid copolymer S (Eudragit S (trade name) Rohm Pharma); shellac, etc. Natural products.

  These coating bases may be coated singly or as a mixture of two or more at an appropriate ratio, or two or more may be sequentially coated.

  In the preparation of the present invention, the content of the extract of Korean ginseng water extract is generally about 0.001 to 99% by mass, preferably about 0.01 to 80% by mass, and more preferably about 0.000% by mass with respect to the entire formulation. It is an arbitrary range of 1 to 50% by mass.

  The dose of the extract of D. gondii extract used in the preparation of the present invention is within the range of an effective amount that suppresses weight gain or body fat accumulation of the extract of D. gondii extract, or within the range of an effective amount that reduces blood triglycerides. It may be sufficient, and may be appropriately increased or decreased depending on the severity, individual differences among patients, administration methods, administration periods, and the like. For example, when administered to an adult for the purpose of suppressing body weight gain, it varies depending on the subject of administration, dosage form, food intake, etc. The dry weight is 0.1 to 2000 mg, preferably 0.1 to 800 mg, more preferably 0.1 to 400 mg, particularly preferably 1 to 100 mg, and most preferably 5 to 60 mg. In addition, when administered to adults for the purpose of improving hypertriglyceridemia, it varies depending on the state of the administration subject, administration form, food intake, etc. The dry weight per kg body weight is 0.1 to 2000 mg, preferably 0.1 to 800 mg, more preferably 0.1 to 400 mg, particularly preferably 1 to 100 mg, and most preferably 5 to 60 mg. In addition, although the said amount may be administered at once, it may be divided into several times. If the dose of the extract of the extract of Korean ginseng extract is too small, the effect is not exhibited, and if it is too large, the absorption of other nutritional components such as fat-soluble vitamins may be inhibited. Moreover, each dosage illustrated above is preferable also from a viewpoint that an effect expresses without giving influence to palatability and food intake. Similar amounts can be administered for other animals.

  The administration method of the preparation of the present invention is not particularly limited, and generally includes administration methods by oral administration as described above. Depending on the patient's condition, severity, etc., parenteral administration (intravascular administration) (Intravenous, intraarterial) administration, subcutaneous administration, rectal administration, etc.) may be appropriately selected.

The preparation of the present invention can suppress the accumulation of body fat, particularly visceral fat, and in particular, suppresses an increase in the amount of neutral fat in the blood immediately after a meal and returns to a normal value over time. Therefore, the absorption of fat ingested by meals can be suppressed, and obesity can be prevented, ameliorated, and / or treated. As a result, various diseases associated with obesity (for example, metabolic syndrome such as diabetes, hyperlipidemia, arteriosclerosis, cerebral infarction, myocardial infarction) can be prevented, improved, or treated.
Moreover, since it has a favorable lowering | reducing effect with respect to the neutral fat (triglyceride) in a blood, the state where the neutral fat (triglyceride) in a blood is high can be made into a normal state. Therefore, the prevention, improvement or therapeutic effect of hyperlipidemia is excellent, and the risk of developing CAD can be reduced well.

  In addition, the extract of water extract used as an active ingredient in the preparation of the present invention is one or more other anti-obesity agents, appetite suppressants, diabetes treatment agents, diabetic complication treatment agents, antihyperlipidemic agents. , And can be used in combination with drugs such as antihypertensives and diuretics (hereinafter abbreviated as concomitant drugs). In the present specification, “used in combination” may be either a form administered as separate preparations or a form combined as one preparation.

  When used in combination as separate preparations, the time of administration of the extract of the kojimoshi water extract and the concomitant drug used in the preparation of the present invention is not limited, and these may be administered simultaneously to the administration subject, May also be administered. Furthermore, two or more types of concomitant drugs may be used in combination at an appropriate ratio.

(2) Food composition of the present invention When using the kojimoshi water extract as the above-mentioned food composition, it can be carried out by a usual method. Specifically, for example, it can be carried out as described below.

  The food composition of the present invention is not particularly limited as long as it contains an extract from water extract of the Korean dung bean and can be taken orally by animals, and the type and shape of the food composition are not particularly limited. Examples of the food composition include confectionery such as drop, candy, ramune, gummy and chewing gum; Western confectionery such as cookie, cracker, biscuit, potato chips, cake, chocolate, donut, pudding and jelly; Japanese confectionery such as rice cakes, buns and castella; frozen confectionery such as ice cream, ice candy, sherbet and gelato; breads such as bread, French bread and croissants; noodles such as udon, soba noodles, Chinese noodles and kishimen; Fish meat products such as ham, sausage, hamburger, corn beef, etc .; salt, pepper, miso, soy sauce, sauce, dressing, mayonnaise, ketchup, sweetener (eg sugar, honey, powdered candy, syrup, jam, Marmalade) Seasonings such as seasonings (eg, mustard, pepper, etc.); Teppanyaki foods such as Akashi-yaki, Takoyaki, Monjayaki, Okonomiyaki, Yakisoba, Yaki Udon; Dairy products such as cheese, butter, margarine, yogurt; Natto, thickness Fried, tofu, konjac, dumplings, pickles, boiled rice, dumplings, shumai, croquettes, sandwiches, pizza, hamburgers, salads, etc .; beef, pork, chicken and other livestock products; shrimp, scallops, salmon, kelp, etc. Marine products; various powders made from vegetables / fruits, plants, yeasts, algae, etc .; oils and fragrances (vanilla, citrus, bonito, etc.) powdered and solidified; beverages and the like.

  Beverages include food and drink such as soup and miso soup; powdered food and drink such as instant coffee, instant tea, instant milk, instant soup and instant miso soup; whiskey, bourbon, spirits, liqueur, wine, fruit liquor, sake, Chinese liquor, Alcoholic beverages such as shochu, beer, non-alcohol beer with alcohol content of 1% or less, sparkling liquor, strawberry high; beverages containing fruit juice (eg, apple, mandarin, grape, banana, pear, ume juice, etc.), vegetable juice (eg, , Tomato, carrot, celery, cucumber, watermelon vegetable juice, beverages containing fruit juice and vegetable juice, soft drinks, milk, soy milk, milk beverages, drink-type yogurt, coffee, cocoa, tea beverages (tea, Green tea, barley tea, brown rice tea, sencha, gyokuro tea, roasted tea, oolong tea, turmeric tea, tea Al tea, Rooibos tea, rose tea, chrysanthemum tea, herbal tea (for example, mint tea, jasmine tea), etc.), energy drinks, sports drinks, non-alcoholic beverages such as mineral water and the like.

  Preferable examples of such food compositions include jelly, tea beverage, alcoholic beverage drop, candy, ramune, cookie, cracker, biscuit, chocolate, cheese, butter, margarine, chewing gum and the like.

  In addition, the food composition of the present invention can be used as functional foods, health foods, foods for specified health use, health function foods such as functional nutrition foods, special use foods (for example, foods for patients), health supplements, supplements, etc. It may be prepared and is preferably prepared as a food for specified health use, a food for special use, a health supplement or a supplement.

  Examples of the shape of the food composition of the present invention include tablets, rounds, capsules (including hard capsules, soft capsules, and microcapsules), powders, granules, fine granules, troches, and liquids (syrups). Shape, milky form, suspension form, etc.), etc., and tablet form and capsule form are preferred.

  The food composition of the present invention is particularly preferably a tablet-like or capsule-like food for specified health use, special food, health supplement or supplement.

  In this specification, a supplement means not only a dietary supplement for supplementing nutrients and the like, but also functions useful for maintaining, recovering, and promoting health (for example, suppression of weight gain, suppression of body fat accumulation). It also means a health functional food or the like having an anti-obesity action or slimming action.

  The food composition of the present invention can be produced, for example, by adding an extract of Korean ginger water extract to food by a known method. Specifically, for example, a tablet-like food composition is added with raw materials such as, for example, an extract from daturated water and excipients (for example, lactose, sucrose, mannitol, etc.), sweeteners, and flavoring agents. It can be produced by mixing and molding it into a tablet by applying pressure with a tableting machine or the like. Other materials (for example, vitamins such as vitamin C, minerals such as iron, dietary fiber, etc.) can be added as necessary. Capsule-like food compositions are prepared by filling a capsule with a liquid, suspension, paste, powder, or granule food composition containing, for example, an extract of water extract of Korean ginseng, or covered with a capsule base. It can be manufactured by encapsulating.

  As long as the effects of the present invention are not hindered, the food composition of the present invention can be used in addition to commonly used food materials, food additives, various nutrients, vitamins, flavor substances (for example, cheese and chocolate), etc. Can be blended with an acceptable carrier. As physiologically acceptable carriers, etc., various conventional organic or inorganic carrier materials are used, and excipients, binders, disintegrants, lubricants, coloring agents, sweeteners, preservatives, antioxidants, A thickener, an emulsifier, etc. are mentioned. Examples of food additives include colorants, sweeteners, preservatives, antioxidants, and flavoring agents. Furthermore, other materials, for example, minerals such as iron, dietary fibers such as pectin, carrageenan and mannan may be contained.

  Excipients, binders, disintegrants, lubricants, solvents, solubilizers, suspending agents, buffers, thickeners, colorants, sweeteners, preservatives, and antioxidants are described above. The thing similar to what is used for the formulation of this invention is mentioned.

  Vitamins may be water-soluble or fat-soluble, such as retinol palmitate, tocopherol, bisbenchamine, riboflavin, pyridoxine hydrochloride, cyanocobalamin, sodium ascorbate, cholecalciferol, nicotinamide, pantothene. Examples include calcium acid, folic acid, biotin, and choline bitartrate.

  For tablet-like, granule-like, fine-grained food compositions, etc., coating is carried out by a known method using a coating substrate for the purpose of masking taste, improving light stability, improving appearance or enteric properties, etc. May be. Examples of the coating substrate include those similar to those used in the preparation of the present invention described above, and can be carried out in the same manner.

  The content of the extract of the water extract in the food composition of the present invention is usually about 0.1 to 20% by mass, preferably about 0.5 to 10% by mass, more preferably based on the whole food composition. , About 1-5% by mass. If the content of the extract of the water extract of Korean ginseng is too small, the effect is not manifested, and if it is too large, the absorption of other nutritional components such as fat-soluble vitamins may be inhibited.

  In addition, when the food composition contains fat, the content of the extract of kojimoshi water extract with respect to the fat in the food composition of the present invention is usually 1 to 30% by mass, preferably 5 to 20% by mass, Preferably it is the range of 10-15 mass%. If the content of the extract of the water extract of Korean ginseng is too small, the effect is not manifested, and if it is too large, the absorption of other nutritional components such as fat-soluble vitamins may be inhibited.

  Since the food composition thus obtained is safe, it can be continuously given to vertebrates, preferably mammals, particularly preferably humans.

  The intake amount of the food composition of the present invention may be within the range of an effective amount that suppresses the increase in body weight and body fat accumulation of the extract of water extract of the ginseng water, or the effective amount that reduces blood triglycerides. For example, when the food composition of the present invention is ingested by an adult for the purpose of suppressing weight gain, the amount of the extract of the extract of kojimoshi water is generally one day, depending on the subject to be ingested, the intake form, the amount of food intake, etc. Per kg of body weight, the dry weight is 0.1 to 800 mg, preferably 0.1 to 400 mg, more preferably 1 to 400 mg, and particularly preferably 40 to 400 mg. In addition, in the case of ingestion by adults for the purpose of suppressing blood triglyceride rise and improving hypertriglyceridemia, it depends on the condition of the subject to be ingested, the form of intake, the amount of food intake, etc. Generally, the dry weight per day per kg of body weight is 0.1 to 800 mg, preferably 0.1 to 400 mg, more preferably 1 to 400 mg, and particularly preferably 40 to 400 mg. In addition, although the said amount may be ingested once, you may ingest in several times. If the dose of the extract of the extract of Korean ginseng extract is too small, the effect is not exhibited, and if it is too large, the absorption of other nutritional components such as fat-soluble vitamins may be inhibited. Moreover, each said intake is preferable also from a viewpoint that an effect expresses without giving influence to palatability and food intake. Similar amounts can be taken for other animals.

  When adding the extract of Korean ginseng water extract to health supplements, health functional foods such as foods for specified health use, special purpose foods such as foods for the sick, It is preferable to contain in the drink or food of the form packaged or filled by the unit of 1 meal intake. Here, “a beverage or food in a form packaged or filled in a single food intake unit” means that the amount of beverage or food to be ingested at a time is packaged in a single bag, box, bottle or other container. Or it means being filled.

  Furthermore, although the food composition of the present invention may be used alone, it may be used in combination with other pharmaceutical compositions, food compositions, or feeds having an anti-obesity effect or blood triglyceride lowering action. . By using in combination, the anti-obesity effect such as weight gain inhibitory effect and body fat accumulation inhibitory effect, slimming effect or prevention or improvement effect of hypertriglyceridemia can be further enhanced.

  In addition, the food composition of the present invention includes health foods, functional foods, health supplements, health functional foods such as foods for specified health use, foods with a disease risk reduction label, or special-purpose foods (for example, for the sick Food)) is also included. As the disease risk reduction indication, for example, an indication that it is for treating, improving, suppressing, and / or preventing obesity, or an indication that it is for improving and / or preventing hypertriglyceridemia Is mentioned. Therefore, the composition of the present invention is, for example, for improving and / or preventing obesity containing an extract of water extract of datura, for slimming, or for improving and / or preventing hypertriglyceridemia. It is a food and drink with an indication that it is intended to do.

  Here, the function indication given to these food compositions can be any of the main body of the product, the container, the packaging, the instructions, the package insert, or the promotional material.

  The present invention will be described more specifically with reference to the following examples, test examples, and formulation examples, but the present invention is not limited thereto.

Example 1: Preparation of an extract of water extract from Kojisen (digestion method)
Ten times the amount of water was added to Gomi, boiled and extracted at 100 ° C. for 1 hour, the crude drug residue was removed by filtration, and the resulting aqueous solution was used as an extract of Korean ginseng water.

Example 2: Preparation of a water extract of Korean ginseng (cold soaking method)
Ten times the amount of water was added to Goriko, extracted overnight (8-12 hours) at room temperature, the crude drug residue was removed by filtration, and the resulting aqueous solution was used as an extract of Kojimoshi water.

Example 3: Preparation of an extract of aqueous ginseng water extract (powder) The extract of aqueous ginseng water extract obtained in Example 1 or 2 is dried by concentration under reduced pressure, freeze-dried, or spray-dried by a spray drier to form a powder. What was used was used.

Test Example 1: Effects on the body weight gain of rats by using the extract of Kojimoshi water extract (1) Experimental method Rats (male, 5 weeks old, BrlHan: WIST @ Jcl (GALAS), CLEA Japan, Inc.) for 3 days on a normal diet ( MF: Oriental Yeast Co., Ltd.) was preliminarily bred for 10 weeks, and was fed with feed and water having the composition shown in Table 1 for each group. A group of high-fat meals containing an extract of water extract from Korean ginseng (hereinafter sometimes referred to as “garbage-containing high-fat meal” or “garbage + high-fat meal”) was prepared in Example 1 per gram of high-fat meal. A feed containing 0.05 ml of an extract from Kojisen waste water (corresponding to 0.1 g of gomi) was freely fed. All animals were raised at a temperature of 23 ± 2 ° C., a humidity of 50 ± 10%, and a light / dark cycle of 12 hours (from 8 o'clock to 20 o'clock).

  Daily food intake measurements up to 10 weeks were measured for the normal diet group (6 animals / group), the high fat diet group (control group) (6 animals / group), and the high fat diet group containing trash (6 animals / group). The body weight was measured every two weeks.

(2) Result It was shown that the body weight increase was suppressed in the high-fat diet group containing garbage, compared with the control group (FIG. 1). In addition, the energy intake (food intake) converted according to the normal food calorie table showed no significant difference in any of the high fat diet group and the garbage mixed high fat diet group (FIG. 2).

Test Example 2: Oral glucose tolerance test (OGTT) on high-fat diet-fed rats combined with extract from water extract
(1) Experimental method Rats (male, 5 weeks old, BrlHan: WIST @ Jcl (GALAS), CLEA Japan, Inc.) were allowed to freely feed for 10 weeks with feed and water having the composition shown in Table 1 for each group. Feeded and raised. After fasting overnight, the normal diet group (6 animals / group), the high fat diet group (control group) (6 animals / group), and the high fat diet group (6 animals / group) containing trash were sugar-resistant by OGTT. Performance was measured. The sugar load was 1 g / kg. Blood glucose was measured from the tail vein immediately before, 30, 60, 90, and 120 minutes after the glucose load, and the blood glucose level was measured from the tail vein (Glucose Pilot: Awenitir Biotech, Carlsbad). , CA, USA).

(2) Result It was shown that the glucose concentration in the blood 90 minutes later was significantly and significantly reduced in the high-fat diet group containing trash as compared with the control group (FIG. 3). From this fact, it was found that the glucose tolerance is improved by blending the extract of the water extract of Korean ginger.

Test Example 3 Effect on Rat Blood Lipid Levels Containing Extract Extract from Daturated Water (1) Experimental Method Rats (male, 5 weeks old, BrlHan: WIST @ Jcl (GALAS), Claire Japan) for 10 weeks Each group was fed with feed and water having the composition shown in Table 1 and fed freely. Blood was collected from rats after 10 weeks for the normal diet group (6 animals / group), the high fat diet group (control group) (6 animals / group), and the high fat diet group (6 animals / group) mixed with garbage. Then, the total cholesterol level and the neutral fat (triglyceride (TG)) level in the blood were measured. The total cholesterol level is measured with a measurement kit (Cholesterol E-Test Wako, Wako Pure Chemical Industries, Ltd.), and the TG level is measured with a measurement kit (Triglyceride E-Test Wako, Wako Pure Chemical Industries, Ltd.). It was.

(2) Result It became clear that the amount of total cholesterol in blood (FIG. 4), especially the amount of TG (FIG. 5) decreased significantly in the high-fat diet group containing garbage.

Test Example 4: Effect on Visceral Fat Content of Rats Containing Extract Extract from Datura (1) Experimental Method Rats (male, 5 weeks old, BrlHan: WIST @ Jcl (GALAS), Claire Japan, Inc.) for 10 weeks Each group was fed with feed and water having the composition shown in Table 1 and fed freely. The normal diet group (6 animals / group), the high fat diet group (control group) (6 animals / group), and the high fat diet group (6 animals / group) mixed with garbage were sacrificed after 10 weeks, and the tissue weight ( Measurement of liver, kidney, and epididymal adipose tissue).

(2) Results The tissue weight of visceral fat after 10 weeks in the high-fat diet group containing the garbage was significantly smaller than that of the control group, and almost equal to that of the normal diet group despite having eaten the high-fat diet. A similar weight was shown (FIG. 6).

Test Example 5: Action on rat leptin level by combination with extract of extract of water extract of datura (1) Experimental method Rat (male, 5 weeks old, BrlHan: WIST @ Jcl (GALAS), Nippon Claire Co., Ltd.) for 10 weeks Each group was fed with feed and water having the composition shown in Table 1 and fed freely. Blood was collected from rats after 10 weeks for the normal diet group (6 animals / group), the high fat diet group (control group) (6 animals / group), and the high fat diet group (6 animals / group) mixed with garbage. Then, the amount of leptin in the blood was measured. The measurement was performed using a kit (leptin measurement kit, Morinaga Institute of Science).

(2) Results The amount of leptin in blood after 10 weeks in the high-fat diet group with garbage was significantly lower than that in the control group, and it was almost the same as that in the normal diet group even though the high-fat diet was consumed. It was shown that it becomes the blood level of (Figure 7). Leptin is an adipocytokine that is secreted from adipose tissue and is known to increase in secretion due to obesity, but because of the decrease in secretion in the high-fat diet group with garbage, garbage suppresses leptin secretion It was shown to have an effect.

Test Example 6: Effect on the adipose tissue hypertrophy of rats by the extract of the extract of datura water (1) Experimental method Rats (male, 5 weeks old, BrlHan: WIST @ Jcl (GALAS), Claire Japan) 10 Each group was fed with feed and water of the composition shown in Table 1 for each group for free, slaughtered after 10 weeks, dissection, and then collected epididymal fat tissue (visceral fat) 2 Soaked in 10% formaldehyde for a day. After embedding with paraffin, it was sliced and pasted on a slide glass. Paraffin sections attached to a slide glass were deparaffinized with xylene and ethanol, and then stained with hematoxylin and eosin (HE). The tissue section was covered with a mounting medium, and observed under ZEISS AXIOSCOPE (manufactured by ZEISS) at a magnification of 100 times.

(2) Results As shown in FIG. 8, the size of fat cells forming the visceral adipose tissue of the high fat diet fed group was almost 100 μm or larger, whereas the fat cells contained high fat. In the diet group, the size of the adipocytes was 100 μm or less as in the normal diet group, and no enlargement was observed. Therefore, it is considered that garbage caused the fat cell hypertrophy and accumulation for some reason, and as a result, the weight gain was suppressed. Since visceral fat is thought to cause lifestyle-related diseases such as diabetes, hypertension, and hyperlipidemia, hypertrophy and / or suppression of accumulation of fat cells in visceral adipose tissue of garbage is a cause of lifestyle-related diseases. It is thought to lead to prevention.

Test Example 7: Action of Korean extract extract on db / db mouse blood triglyceride (1) Experimental method A db / db mouse (male, 14 weeks old, CLEA Japan) was used as a control group (n = 6) and trash extract. Divided into groups (n = 6), the control group was orally administered 10 mg / g / day of an extract of daturated water in addition to the normal food for the control group and the normal meal for the garbage extract group, and was bred for 34 days. Body weight and intake were measured every 3 days, fasted for 1 day after completion of the test, and blood neutral fat (triglyceride; TG) value was measured by the same method as described above.

(2) Results In db / db mice lacking the leptin receptor and developing type II diabetes, there was no difference between the intake of the garbage extract group and the intake of the control group (FIG. 9). On the other hand, as shown in FIG. 10, blood triglyceride (TG) value was significantly decreased in the garbage extract group (p <0.005).
That is, in the db / db mouse, which is a type II diabetes model animal with obesity, the extract of D. gondii water shows an effect of significantly suppressing the increase of blood neutral fat (triglyceride), and the high triglyceride blood It has been shown to be effective in preventing and ameliorating symptoms and reducing the risk of developing CAD.

Test Example 8: Effect of extract of D. gondii extract on blood neutral fat (triglyceride) in lipid-ingested rats (1) Experimental method
Wister rats (male, 6 weeks old, Japan Claire Co., Ltd.) were divided into control group (n = 6) and garbage extract group (n = 6). A water extract (5 g / kg in crude drug amount) was orally administered, and immediately after that olive oil (5 ml / kg) was orally administered to both groups, and the blood neutral fat (triglyceride) concentration over time was determined as above. It measured by the same method.

(2) Results As shown in FIG. 11, the triglyceride extract group has a significantly reduced blood neutral fat concentration compared to the control group (p <0.01), and the neutral blood concentration after oral administration of olive oil. The increase in fat concentration was also significantly suppressed (p <0.001).
That is, it has been shown that the extract of Kojisenshishi water suppresses an increase in blood neutral fat (triglyceride) concentration during lipid intake.

Formulation Example 1: Manufacture of liquid preparation (1) 1 g of the extract of Korean ginger water extract of Example 1 or 2
(2) Isomerized sugar 10g
(3) Mannitol 5g
(4) Purified water Appropriate amount In accordance with the usual method for producing a liquid preparation, each component is added and dissolved in purified water. The total volume is adjusted to 100 ml, and then filled into brown bottles and sterilized to produce a solution.

Formulation Example 2: Manufacture of powder (1) Chestnut waste water extract (powder) of Example 3 10 mg
(2) Lactose 100mg
(3) Talc 10mg
The above components (1) to (3) are mixed and filled in an airtight packaging material to produce a powder.

Formulation Example 3: Manufacture of Tablets (1) Extract from Korean ginseng water extract (powder) of Example 3 5 mg
(2) Corn starch 30mg
(3) Carboxymethylcellulose calcium 20mg
(4) Microcrystalline cellulose 30mg
(5) Polyvinylpyrrolidone 10mg
(6) Talc 5mg
After mixing said component (1)-(6), according to the normal tablet manufacturing method, it tablets and manufactures a tablet.

Formulation Example 4: Manufacture of capsules (1) Chrysanthemum waste water extract of Example 3 (powder) 10 mg
(2) Corn starch 100mg
(3) Lactose 100mg
(4) Magnesium stearate 2mg
According to the usual capsule manufacturing method, the above components (1) to (4) are mixed and filled into gelatin capsules to prepare capsules.

Formulation Example 5: Manufacture of functional food in the form of a tablet (1) 60 mg
(2) Lactose 55mg
(3) Corn starch 100mg
(4) Microcrystalline cellulose 30mg
(5) Magnesium stearate 5mg
1 tablet 250mg
The above components (1) to (4) are appropriately added while heating and granulating using water as a binder, and (5) is added to obtain a mixture. Thereafter, the tablet is compressed to 250 mg per tablet to obtain a tablet-like functional food.

Formulation Example 6: Production of functional food in the form of a hard capsule (1) Extract from Korean ginseng water extract (powder) of Example 3 60 mg
(2) Lactose 60mg
(3) Microcrystalline cellulose 70mg
(4) Magnesium stearate 10mg
1 capsule 200mg
After mixing 1/2 of the above components (1) to (3) and (4), granulate. The remaining (4) is added to this and the whole is encapsulated in a hard gelatin capsule made of pullulan, vegetable oil, carrageenan, and potassium chloride to obtain a functional food in the form of a hard capsule.

Formulation Example 7: Production of functional food in the form of a soft capsule (1) Extract from Korean ginseng water extract of Example 1 or 2 60 mg
(2) Medium chain triglyceride 320mg
(3) Carnauba wax 20mg
1 capsule 400mg
After mixing the above components (1) to (3), the whole is encapsulated in a soft gelatin capsule mainly composed of carrageenan, starch and glycerin to obtain a functional food in the form of a soft capsule.

Formulation Example 8: Production of granular functional food (1) Extract of Kytochobori water extract (powder) of Example 3 50 mg
(2) Dextrin 450mg
(3) Water proper amount 1 stick 500mg
9 kg of (2) is added to 1 kg of (1), and the mixture is uniformly mixed, heated and granulated using a fluidized bed granulator using (3) as a binder to obtain 10 kg of a granulated product. This granulated product is filled with a stick filling machine so as to be 500 mg per stick to obtain a granular functional food.

Formulation Example 9: Production of functional food in powder form (1) 50 g of the extract of Kojimoshi water extract (powder) of Example 3
(2) Dextrin 450mg
(3) Water proper amount 1 stick 500mg
9 kg of (2) is added to 1 kg of (1), and the mixture is uniformly mixed, heated and granulated using a fluidized bed granulator using (3) as a binder to obtain 10 kg of a granulated product. This granulated product is pulverized until it passes through the entire No. 18 sieve, and filled with a stick filling machine so as to be 500 mg per stick to obtain a powdered functional food.

Formulation Example 10: Production of liquid functional food (1) 6 g ginseng extract extracted from Example 1 or 2
(2) Glucose 700g
(3) Purified water 5.3kg
The above components (1) to (3) and 5 g of fragrance are mixed, and after heat sterilization, each bottle is aseptically filled in a 60 ml sealed container to obtain a liquid functional food.

Formulation Example 11: Production of gel-like functional food (1) Korean ginseng water extract of Example 1 or 2 60 g
(2) 1kg κ carrageenan
(3) Glucose fructose liquid sugar 17kg
(4) Citric acid 0.36kg
(5) Sodium citrate 0.24kg
(6) 82kg of purified water
After mixing said component (1)-(6), it heats at 80 degreeC and fills with a sealed container. Then, after heat sterilization according to a conventional method, the mixture is cooled to obtain a gel-like functional food.

Formulation Example 12: Manufacture of beverages (1) 1 g of the extract of Korean ginseng water of Example 1 or 2
(2) Stevia 0.2g
(3) 3 g of malic acid
(4) Fragrance 0.1g
(5) 45.7 g of purified water
The components (1) to (4) are stirred and dissolved in a part of the component (5). The remainder of component (5) is then added and mixed, heated to 90 ° C. and filled into a 50 mL glass bottle.

Formulation Example 13: Manufacture of confectionery (cookies) (1) 10 mg of extract of Koji Senshi water extract of Example 3 (powder)
(2) Soft flour 32.0g
(3) Whole egg 16.0g
(4) Butter 16.0g
(5) Sugar 25.0g
(6) Water 10.7g
(7) Baking powder 0.29g
A cookie is manufactured according to a conventional method using the above components (1) to (7).

  The pharmaceutical composition or food composition of the present invention containing an extract of kojisenshi water extract as an active ingredient exhibits an excellent effect of suppressing body weight gain and body fat accumulation, has a low risk of side effects and is safe and long. Since it can be administered for a period or fed, it is useful for the prevention, amelioration, or treatment of obesity. The pharmaceutical composition or food composition of the present invention can be useful for slimming applications, as well as for preventing, improving, or treating various metabolic syndromes induced by obesity.

  Further, the pharmaceutical composition or food composition of the present invention has an effect of satisfactorily lowering the triglyceride against an increase in blood neutral fat (triglyceride) that occurs regardless of obesity or accumulation of body fat. It is useful for prevention, improvement, or treatment of triglyceridemia, and thus can reduce the risk of developing coronary artery disease (CAD). Furthermore, the pharmaceutical composition or food composition of the present invention has a good lowering effect on blood triglycerides accompanying lipid intake.

Claims (36)

  An anti-obesity agent containing an extract of Kojisen waste water as an active ingredient.   The anti-obesity agent according to claim 1, which comprises an extract from Kytocho-sei.   The water extract of Korean ginseng extract is an aqueous solution obtained by adding water to a dried dried fruit of ginseng, extracting at 60 ° C. to 100 ° C. for 0.5 to 8 hours, and removing the residue by filtration. Item 3. The anti-obesity agent according to Item 1 or 2.   4. The extract of Korean ginseng water extract is an aqueous solution obtained by adding water to a dried dried fruit of ginseng, extracting at 80 ° C. to 100 ° C. for 1 to 4 hours, and removing the residue by filtration. The antiobesity agent described in 1.   5. The anti-Korean trash extract is an aqueous solution obtained by adding water to a dried matured fruit of B. rubric, boiling and extracting at 100 ° C. for 1 hour, and removing the residue by filtration. Obesity agent.   The extract of Korean ginseng water extract is an aqueous solution obtained by adding water to a dried mature fruit of ginseng, cooling at room temperature for 8 to 12 hours, and removing the residue by filtration. Anti-obesity agents.   The anti-obesity agent according to any one of Claims 3 to 6, wherein the extract of D. chinensis water is a powder obtained by drying the aqueous solution.   A weight gain inhibitor comprising the anti-obesity agent according to any one of claims 1 to 7.   The weight gain inhibitor according to claim 8, wherein the weight gain is weight gain before reaching obesity.   The weight gain inhibitor according to claim 8, wherein the weight gain is a weight gain of an obese patient.   The body weight gain inhibitor according to any one of claims 8 to 10, wherein the content of the extract of the water extract of Korean ginseng is an effective amount for suppressing weight gain.   The body weight gain inhibitor according to claim 11, wherein the effective amount is 0.1 mg to 2000 mg / kg body weight / day as a dry weight.   The body fat increase inhibitor which consists of an antiobesity agent as described in any one of Claims 1-7.   The body fat increase inhibitor according to claim 13, wherein the increase in body fat is an increase in neutral fat.   A blood triglyceride-lowering agent containing an extract from Kojisen wastewater as an active ingredient.   The blood triglyceride lowering agent according to claim 15, which comprises an extract from a dung beetle water.   The water extract of Korean ginseng extract is an aqueous solution obtained by adding water to a dried dried fruit of ginseng, extracting at 60 ° C. to 100 ° C. for 0.5 to 8 hours, and removing the residue by filtration. Item 15. A blood triglyceride lowering agent according to Item 15 or 16.   18. The extract of Korean ginseng water is an aqueous solution obtained by adding water to a dried dried fruit of Korean ginseng, extracting at 80 ° C. to 100 ° C. for 1 to 4 hours, and removing the residue by filtration. The blood triglyceride lowering agent according to 1.   The blood of Claim 18 is an aqueous solution obtained by adding water to a dried dried fruit of Korean ginseng, boiled and extracted at 100 ° C for 1 hour, and removing the residue by filtration. Medium triglyceride lowering agent.   The extract of Korean ginseng water extract is an aqueous solution obtained by adding water to a dried mature fruit of ginseng, cooling at room temperature for 8 to 12 hours, and removing the residue by filtration. Blood triglyceride lowering agent.   21. The blood triglyceride lowering agent according to any one of claims 17 to 20, wherein the extract of Datura communis water is a powder obtained by drying the aqueous solution.   The therapeutic agent for hypertriglyceridemia containing the blood triglyceride lowering agent as described in any one of Claims 15-21.   23. Formulation according to any one of claims 1 to 22, characterized in that it is prepared as a tablet, round, capsule, powder, granule, fine granule, troche or liquid form. .   The preparation according to any one of claims 1 to 23, which is for oral administration.   Furthermore, it is used in combination with one or more drugs selected from the group consisting of anti-obesity agents, appetite suppressants, diabetes treatment agents, diabetic complication treatment agents, antihyperlipidemic agents, antihypertensive agents, and diuretics. 25. A formulation according to any one of claims 1 to 24, characterized in that   A food composition comprising the anti-obesity agent according to any one of claims 1 to 7, or the blood triglyceride lowering agent according to any one of claims 15 to 21.   27. The food composition according to claim 26, wherein the content of the extract of Koji Senshi water extract is 1 to 5% by mass relative to the total amount of the food composition.   The supplement, functional food, health food, enteral nutrition food, special-purpose food, health supplement food, food for specified health use, or functional nutrition food, characterized in that it is prepared as claimed in claim 26 or 27. Food composition.   The food composition according to claim 28, which is a special-purpose food or a food for specified health use.   30. The food composition according to claim 29, wherein the special purpose food is a food for a sick person.   The food according to any one of claims 26 to 30, wherein the food is prepared as a tablet, round, capsule, powder, granule, fine granule, troche, or liquid form. Composition.   Prepared as beverages, confectionery, frozen confectionery, noodles, processed fishery products, livestock processed foods, dairy products, processed oils and fats, processed foods, seasonings, enteral nutrition, soups, stews, prepared dishes, pickles, or bread The food composition according to any one of claims 26 to 30, wherein   A food for specified health use, which contains an extract of water extract from Korean ginseng, for preventing or improving obesity, for suppressing weight gain, for suppressing body fat accumulation, or for slimming.   A special-purpose food for preventing or improving obesity, for suppressing body weight gain, for suppressing body fat accumulation, or for slimming, containing an extract from Korean ginseng water.   A special health food for preventing or improving hypertriglyceridemia, which contains an extract from Korean ginseng water.   A special-purpose food for preventing or ameliorating hypertriglyceridemia, which contains an extract from Korean ginseng water.