JP2011503019A - 薬学的使用のための新規のニュールツリン複合体 - Google Patents
薬学的使用のための新規のニュールツリン複合体 Download PDFInfo
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- JP2011503019A JP2011503019A JP2010532485A JP2010532485A JP2011503019A JP 2011503019 A JP2011503019 A JP 2011503019A JP 2010532485 A JP2010532485 A JP 2010532485A JP 2010532485 A JP2010532485 A JP 2010532485A JP 2011503019 A JP2011503019 A JP 2011503019A
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Abstract
Description
et al., 2003, Nat Rev Immunol. 3: 123-132;Homann et
al., Immunity. 2002, 3: 403-415)。しかしながら、ヒトβ細胞の相対的に遅い再生のため、このような治療は、それらが、β細胞再生を刺激し得る治療と組合される場合、よりうまくいく。
(発明の詳細な説明)
Nieforth) Dekker, New York, 1997, pp 209)、硫酸ポリグリセリン(Turk,
H., Haag, R., Alban, S. Bioconjugate Chem. 2004, 15, 162)、ポリ硫酸ペントサン(PPS)およびその誘導体、例えばラクトース修飾ポリ硫酸ペントサン、分別PPS/低分子量PPS、フコイダン、あるいはその誘導体または組合せから選択される。
(1992(249-304))も参照)。
(1997) 218-238に従って実施され得る。選択的N末端PEG化は、N−PEG化アミノ酸誘導体をペプチド鎖のN末端アミノ酸と結合することにより、固相合成中に達成される。側鎖PEG化は、N−PEG化アミノ酸誘導体を成長中の鎖と結合することにより、固相合成中に実施される。併合N末端および側鎖PEG化は、固相合成内で上記のように、またはアミノ脱保護化ペプチドに活性化PEG試薬を適用することにより液相合成によって、プロセシングされる。
ポリエチレングリコール基(PEG)を、PEG化と呼ばれるプロセスによりニュールツリンと共役させた。この技術は治療用タンパク質の修飾のために広範に用いられ、当該手順は当業者によく知られている。本発明においては、単一PEG分子を、ホモ二量体の2つのサブユニットのうちの1つのN末端アミノ酸と連結することにより、モノPEG化ニュールツリン複合体を製造した。結合PEGのサイズおよび構造が異なり、ならびにPEGをタンパク質と共役させる製造方法が異なるモノPEG化ニュールツリンの2つの異なる複合体を製造した。約5kDaの線状PEG試薬(mPEG−スクシニミジルスクシネート;NOF, Japan)を用いて、本明細書中で「モノ−mPEG−NHS−ニュールツリン」として開示される複合体を製造した。このいわゆる「NHS法」は、可溶性タンパク質のPEG化のために最も一般的に用いられる方法である。さらに、線状5kDaポリエチレングリコールブチルアルデヒド(Nektar、082M0H01)を用いて、「モノ−mPEG−CHO−ニュールツリン」として開示される複合体を生成した。特定実験条件下で、NHSエステルまたはアルデヒドは、タンパク質およびペプチドの遊離アミノ基と効率的に反応する。「モノ−CES0310−ニュールツリン」として開示されるさらに別の複合体では、ニュールツリンホモ二量体の1つの鎖のN末端アミノ酸を、従来技術で既知のPEG化法を用いて、約5kDaの6腕分枝鎖PEGと共役させた(例えばDE2005 100 04 157.0、EP 1 631 545 A2;W04/108634;WO07/025763;CA2528667(これらに限定されない)参照)。ニュールツリンはリシン残基を含有せず、したがって、上記のPEG化反応は、主に、ニュールツリンホモ二量体のN末端アミノ酸の反応性アミノ基を介して、N末端PEG化をもたらすと予測される。当業者に既知の標準生化学的方法を用いて、PEG化産物の質(サイズおよび純度)を確証した。
実施例2−PEG化ニュールツリン複合体またはその変異体の生物学的活性
実施例3−PEG化ニュールツリン複合体またはその変異体の生物学的利用能の改良
実施例4−ニュールツリン変異体
R51E、R52E、R54E、R56E、R57E、R58E、R60E、R61E、R63EまたはR65E(成熟ヒトニュールツリンペプチドの配列に従って位置に番号を付す。GenBank寄託番号NP_004549、成熟ペプチドaa 96〜197。この配列は図3にも示されている;R:アルギニン;E:グルタミン酸)を導入するミスマッチプライマーを用いてPCRベースの突然変異誘発により、付加的N末端真核生物分泌シグナルペプチドを伴う成熟ヒトニュールツリンのコード配列を修飾した。
Kiuchi K. Heparin facilitates glial cell line-derived neurotrophic factor
signal transduction. Neuroreport. 2002 Oct 28; 13(15): 1913-6;Tanaka M, Xiao H, Hirata Y, Kiuchi K. A rapid assay for glial cell
line-derived neurotrophic factor and neurturin based on transfection of cells
with tyrosine hydroxylase promoter-luciferase construct. Brain Res Brain Res
Protoc. 2003 May; 11(2):119-22)による、ヒトTGW神経芽細胞腫細胞株中で安定的に発現されるニュールツリンによるチロシンヒドロキシラーゼ(TH)レポーター遺伝子構築物の誘導を示す実験に基づいている。要するに、チロシンヒドロキシラーゼ(TH)レポーター遺伝子構築物を過剰発現するTGW細胞を、ニュールツリン含有試料で処理して、ニュールツリンシグナル伝達によるMAPK経路の誘導をもたらす。レポーター遺伝子構築物は、反復血清応答素子(SRE)の制御下でルシフェラーゼ遺伝子を含有する。ルシフェラーゼの発現は、MAPK経路活性化に依っている。96ウエルプレート中で検定を実行する。ニュールツリン媒介性ルシフェラーゼ発現をルシフェリンの付加により測定し、ルミネッセンス読取装置で解析する。
domestica)およびニワトリ(Gallus gallus)のヒトニュールツリン相同体中に存在する、ということを示す。さらにまた、位置52、57、58および61でのアルギニン残基の天然変異体は、異なるニュールツリン相同体中に存在する(図6参照)。
・Kr:ヒトコドン使用のために最適化される細胞質分泌に関するマウスクリングル含有膜貫通タンパク質2、アミノ酸1〜26、シグナルペプチド(GenBank寄託番号NP_082692)のDNA配列(アミノ酸配列:MGTPHLQGFLLLFPLLLRLHGASAGS;配列番号2)。
・G3:ヒトコドン使用のために最適化されるグリシンスペーサーのDNA配列(アミノ酸配列:GGG;配列番号3)。
H6:ヒトコドン使用のために最適化される精製目的のための6−ヒスチジンタグのDNA配列(アミノ酸配列:HHHHHH;配列番号4)。
Xa:ヒトコドン使用のために最適化される因子Xaプロテアーゼ認識部位のDNA配列(アミノ酸配列;IEGR;配列番号5)。因子Xaプロテアーゼはアルギニンの後でC末端を切断する。
rhニュールツリン:ヒトコドン使用のために最適化される成熟組換えヒトニュールツリンのDNA配列(アミノ酸配列:ARLGARPCGLRELEVRVSELGLGYASDETVLFRYCAGACEAAARVYDLGLRRLRQRRRLRRERVRAQPCCRPTAYEDEVSFLDAHSRYHTVHELSARECACV;配列番号6)。位置51、52、54、56、57、58、60、61、63または65の単一アルギニンコドンは、それぞれのニュールツリン変異体発現構築物中のリシンコドンAAGにより置き換えられる。
実施例5−ニュールツリン変異体のPEG化
vivoで検定する。基線での、ならびに投与後0.25、0.5、1、1.5、2、3、4、6および8時間目に、ニュールツリンELISAにより、血漿ニュールツリン濃度を確定する。
実施例6−PEG化ニュールツリンの免疫原性能力
Claims (33)
- ヒトニュールツリンタンパク質産物またはその生物学的活性断片と共有結合されるポリオール部分を含むニュールツリン複合体。
- 配列番号1で記述されるアミノ酸配列によりコードされるポリペプチド、あるいはその相同体、オルソログ、変異体、類似体、誘導体、生物学的活性断片または生物学的活性突然変異体を含む請求項1記載のニュールツリン複合体。
- 前記ポリオール部分がポリエチレングリコール部分である請求項1または2記載のニュールツリン複合体。
- 前記ポリオール部分が一本鎖ポリオール部分である請求項1〜3のいずれか一項に記載のニュールツリン複合体。
- 前記ポリオール部分が分枝鎖ポリオール部分である請求項1〜3のいずれか一項に記載のニュールツリン複合体。
- 前記ポリオール部分がポリアルキレングリコール部分である請求項1〜3のいずれか一項に記載のニュールツリン複合体。
- ネイティブヒトニュールツリンと同一のまたはそれより高いin vivoニュールツリン活性を有する請求項1〜6のいずれか一項に記載のニュールツリン複合体。
- ヒトニュールツリンと比較して少なくとも1つのアミノ酸変化を有する請求項1〜7のいずれか一項に記載のニュールツリン複合体。
- 前記アミノ酸変化が成熟ヒトニュールツリンのアミノ酸47〜69、好ましくは51〜65の間である請求項8記載のニュールツリン複合体。
- アルギニン残基51、52、54、56、57、58、60、61または65のうちの少なくとも1つが欠失されるかまたは置換される請求項8または9のいずれか一項に記載のニュールツリン複合体。
- 少なくとも1つのアルギニン残基が中性または酸性アミノ酸残基、例えばグルタミン酸により置換される請求項10記載のニュールツリン複合体。
- 少なくとも1つのリシン残基が導入される請求項8〜10のいずれか一項に記載のニュールツリン複合体。
- 活性成分としての少なくとも1つのポリエチレングリコール分子と共役する少なくとも1つのニュールツリンタンパク質産物および/またはその生物学的活性断片を製薬上許容可能な担体、希釈剤および/またはアジュバントと一緒に含む製剤組成物。
- 前記ニュールツリンタンパク質産物がヒトニュールツリンタンパク質産物またはその生物学的活性断片である請求項13記載の組成物。
- 前記修飾ニュールツリンタンパク質産物がモノ−PEG化され、単一ポリエチレングリコール分子鎖を含む請求項13または14のいずれか一項に記載の組成物。
- 前記修飾ニュールツリンタンパク質産物がオリゴ−またはポリ−PEG化され、2、3、4つまたは数個のポリエチレングリコール分子鎖を含む請求項13または14のいずれか一項に記載の組成物。
- 前記修飾ニュールツリンタンパク質産物が少なくとも1つの線状ポリエチレングリコール分子鎖を含む請求項13〜16のいずれか一項に記載の組成物。
- 前記修飾ニュールツリンタンパク質産物が少なくとも1つの分枝鎖ポリエチレングリコール分子鎖を含む請求項13〜16のいずれか一項に記載の組成物。
- 前記ポリエチレングリコール分子が100〜10000Daの、好ましくは200〜8000Daの、最も好ましくは1000〜7000Daの平均分子量を有する請求項13〜18のいずれか一項に記載の組成物。
- 前記ポリエチレングリコール分子が前記修飾ニュールツリンタンパク質産物のN末端アミノ酸に連結される請求項13〜19のいずれか一項に記載の組成物。
- 前記ポリエチレングリコール分子がアシルまたはアルキル結合を介して前記修飾ニュールツリンタンパク質産物に連結される請求項13〜20のいずれか一項に記載の組成物。
- 前記ポリエチレングリコール分子がOH−、OCH3−またはOEt−基により終結される請求項13〜21のいずれか一項に記載の組成物。
- 非修飾対照ニュールツリンタンパク質産物と比較して活性成分の生物学的利用能増大を示す請求項13〜22のいずれか一項に記載の組成物。
- 前記修飾ニュールツリンタンパク質が活性成分の生物学的利用能の少なくとも2倍の、好ましくは少なくとも5倍の、さらに好ましくは少なくとも10倍の増大を提供する量で存在する請求項13〜23のいずれか一項に記載の組成物。
- 注射または注入のための請求項13〜24のいずれか一項に記載の組成物。
- 皮下または静脈内注射のための請求項25記載の組成物。
- 膵臓または神経変性障害の予防または治療のための請求項13〜26のいずれか一項に記載の組成物。
- I型真性糖尿病、LADAおよびII型真性糖尿病の予防または治療のための請求項27記載の組成物。
- 哺乳類、特にヒトへの投与のための請求項13〜28のいずれか一項に記載の組成物。
- 野生型ヒトニュールツリンと比較して少なくとも1つの、例えば1、2、3または4つのアミノ酸変化を含むヒトニュールツリンの変異体。
- アミノ酸変化が請求項9〜12に定義されたものと同じである請求項30記載のヒトニュールツリンの変異体。
- 活性成分としての少なくとも1つのポリエチレングリコール分子と共役する少なくとも1つの修飾ニュールツリンタンパク質産物またはその生物学的活性断片を、製薬上許容可能な担体、希釈剤および/またはアジュバントと一緒に含む製剤組成物をそれを必要とする被験者に投与するための方法。
- 活性成分としての少なくとも1つのポリエチレングリコール分子と共役する少なくとも1つの修飾ニュールツリンタンパク質産物またはその生物学的活性断片を、低分子量物質と一緒に、製薬上許容可能な担体、希釈剤および/またはアジュバントと一緒に含む製剤組成物をそれを必要とする被験者に投与するための方法。
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US20050222070A1 (en) | 2002-05-29 | 2005-10-06 | Develogen Aktiengesellschaft Fuer Entwicklungsbiologische Forschung | Pancreas-specific proteins |
ATE435026T1 (de) | 2003-11-27 | 2009-07-15 | Develogen Ag | Verfahren zur prävention und behandlung von diabetes mit neurturin |
CA2778678A1 (en) * | 2009-10-30 | 2011-05-05 | Cns Therapeutics, Inc. | Improved neurturin molecules |
US9127083B2 (en) | 2009-10-30 | 2015-09-08 | Ntf Therapeutics, Inc. | Neurturin molecules |
US8445432B2 (en) | 2009-10-30 | 2013-05-21 | Ntf Therapeutics Inc | Neurturin molecules |
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CN102453725B (zh) * | 2010-11-02 | 2014-06-11 | 杭州纽龙生物科技有限公司 | 一种重组载体、包含该重组载体的重组菌株及制备方法 |
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JP2005527614A (ja) | 2002-05-29 | 2005-09-15 | デヴェロゲン アクチエンゲゼルシャフト フュア エントヴィックルングスビオローギッシェ フォルシュング | 膵臓特異性タンパク質 |
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-
2008
- 2008-11-05 WO PCT/EP2008/009320 patent/WO2009059755A2/en active Application Filing
- 2008-11-05 EP EP08848146A patent/EP2217282A2/en not_active Withdrawn
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- 2008-11-05 KR KR1020107012303A patent/KR20100098619A/ko not_active Application Discontinuation
- 2008-11-05 AU AU2008324426A patent/AU2008324426B2/en not_active Ceased
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WO2012150707A1 (ja) * | 2011-05-02 | 2012-11-08 | 国立大学法人熊本大学 | 幹細胞からインスリン産生細胞への分化誘導を促進する低分子化合物および該化合物を用いた幹細胞からインスリン産生細胞への分化誘導方法 |
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WO2009059755A2 (en) | 2009-05-14 |
JP2015057384A (ja) | 2015-03-26 |
WO2009059755A3 (en) | 2009-09-24 |
US20160060317A1 (en) | 2016-03-03 |
KR20150139982A (ko) | 2015-12-14 |
SG185946A1 (en) | 2012-12-28 |
NZ585262A (en) | 2012-06-29 |
IL205290A (en) | 2014-01-30 |
MX2010004899A (es) | 2010-09-07 |
US20130231283A1 (en) | 2013-09-05 |
BRPI0819220A2 (pt) | 2019-02-26 |
CN101848735B (zh) | 2014-07-23 |
IL205290A0 (en) | 2010-12-30 |
EP2217282A2 (en) | 2010-08-18 |
US20110003741A1 (en) | 2011-01-06 |
AU2008324426A1 (en) | 2009-05-14 |
CA2742839A1 (en) | 2009-05-14 |
KR20100098619A (ko) | 2010-09-08 |
CR11406A (es) | 2010-10-25 |
CN104288775A (zh) | 2015-01-21 |
US9029323B2 (en) | 2015-05-12 |
CO6280503A2 (es) | 2011-05-20 |
AU2008324426B2 (en) | 2014-03-27 |
ZA201003227B (en) | 2011-03-30 |
EA201070484A1 (ru) | 2011-04-29 |
CN101848735A (zh) | 2010-09-29 |
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