JP2011169603A - Specimen analyzing chip and method for manufacturing the same - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a specimen analyzing chip which has little risk of contamination or infection caused by an inspection target solution and has an improved mass productivity. <P>SOLUTION: The specimen analyzing chip which has an elongated flat board shape for analyzing a liquid sample and is constituted by laminating a plurality of sheet members, includes: a positioning hole when inserting the chip in a measuring instrument in the leading end part of the chip; a sample introducing hole at the side surface of the central part protruding therefrom; a sample flow channel for guiding the sample introduced into the chip to a sample storage part by a capillary phenomenon and a membrane filter for treating the sample introduced into the sample storage part; an inspection hole for taking out the treated inspection target solution to inspect under the surface of the chip; and an air hole for smoothing the penetration of the sample at the upper surface of the chip. The end part on the side opposite to the leading end part of the chip forms a grasping part for grasping the chip and water repelling treatment is applied to at least the peripheral part of the sample introducing port of the front and/or back of the chip. <P>COPYRIGHT: (C)2011,JPO&INPIT

Description

  The present invention relates to an analyte analyzing chip for measuring various characteristic values using blood or the like as an analyte.

  Various proposals have been made for blood analysis chips for easily performing various measurements using blood as a subject. Among these chips, the most commonly used chip is a blood sugar level measuring chip.

  Blood analysis chips are roughly classified into two types according to the purpose of use. One is used by individuals to measure their own blood condition, and the other is used to analyze and measure the blood of an unspecified number of people in hospitals and laboratories. is there.

  In the case of chips used by individuals to measure their own blood, contamination and infection problems are hardly a problem, but when handling blood from an unspecified number of people, blood will adhere to the measuring instrument. It is necessary to take some measure to prevent the problem of contamination caused by the blood pressure and the tester from being infected by the blood sample.

  The biosensor test strip described in Patent Document 1 is a blood sugar level analysis chip having a dent on the side surface so that even a diabetic patient with reduced visual acuity can be handled by touch. In the case of a chip provided for such a purpose, no problem is actually taken because the above-mentioned problems relating to contamination and infection do not occur. The same applies to the biosensor described in Patent Document 2.

  The microdevice described in Patent Document 3 is a chip used for the same application as the biosensor described in Patent Document 2, and is a problem of the biosensor described in Patent Document 2, “contact with blood” In order to solve the problem of "the blood sticks to the outer wall surface of the micro device other than the spotted portion when spotted" because the tip of the spotted portion for taking the blood into the inside is rectangular. It was made. In the microdevice of Patent Document 3, as a means for solving this problem, a capillary plate is formed by superimposing a cover plate on a base plate in which a groove serving as a flow path is dug, and the proximal end is the capillary tube. A spotting portion connected to the cavity and having a tip protruding from the cover plate is provided, and the tip of the spotting portion is formed in a hemispherical shape protruding in a direction away from the flow path forming surface of the base plate.

  In the microdevice described in Patent Document 3, a spotted portion is projected, and a rib molded with a resin integrally with the cover plate is provided around the spotted portion, and this rib is the water repellency of the synthetic resin material itself. By playing the blood, the blood is prevented from adhering to a portion other than the spotted portion.

  In the microdevice described in Patent Document 3, it is necessary to form a base plate by resin molding and to separately perform hydrophilic treatment on the inner surface of the flow path so that blood smoothly flows in the flow path formed by resin molding. ,costly. Further, since the water repellency of the rib material itself is used as means for preventing blood from adhering to the periphery of the spotted portion, the water repellency is not sufficient, and the effect of preventing contamination and infection is insufficient.

The test solution analysis chip described in Patent Document 4 has been made mainly to prevent the internal contamination of the measuring instrument and the contamination of the operator, and by devising the shape and structure of the chip. This is an attempt to achieve this goal.

Special table 2001-526388 Japanese Patent Laid-Open No. 2001-159618 JP 2009-229243 JP 2009-175118 A

  Since the test liquid analysis chip described in Patent Document 4 has a structure in which three flat members are bonded together, the structure itself is relatively simple, but in order to form a flow path, Since double-sided adhesive tape punched into the shape of the flow path is used, there is a problem in terms of mass productivity and cost because it is necessary to rely on human manual work for assembly. There is also a problem that blood tends to adhere to the periphery of a contact port for introducing blood as a test solution.

  The present invention has been made in order to solve these problems, and proposes a sample analysis chip that is less likely to be contaminated or infected by a test solution and has good mass productivity.

  As a means for solving the above-mentioned problems, the invention described in claim 1 is an elongated flat plate analysis chip for analyzing a liquid sample, and a plurality of sheet members are bonded together. The tip of the chip has a positioning hole for positioning when inserted into the measuring device, and the side of the center of the chip protrudes from the side of the chip for introducing the specimen sample into the chip. A sample reservoir inside the chip, a sample flow path for guiding the analyte sample introduced from the sample inlet hole to the sample reservoir by capillary action, and a sample introduced into the sample reservoir. It has a membrane filter that processes the sample, and has a test hole on the lower surface of the chip for taking out the processed test liquid and inspecting it, and the upper surface of the chip enters the sample channel of the test sample. To smooth the air The tip end of the tip opposite to the tip forms a grip for gripping the tip, and at least the periphery of the sample introduction hole on the tip surface and / or the back surface is subjected to water repellent treatment. This is an analyte analysis chip.

  The invention according to claim 2 is that the plurality of sheet members are transparent, have a hydrophilic surface, and have an A member having a perforated inspection hole, a membrane filter, and adhesive layers on both surfaces of the substrate. B member from which the portion to serve as the sample flow path and the sample reservoir is punched and removed, a transparent C member having a hydrophilic surface and perforated air holes, and a transparent heat seal on the upper surface. At least a D member having a ruble material layer and air holes perforated, and an E member which is transparent and has a printed layer and a heat sealable material layer on the lower surface and perforated air holes, the A member, the membrane filter, 2. The analyte analyzing chip according to claim 1, wherein B to E members are laminated in this order from the bottom, and a positioning hole is drilled.

  Further, the invention according to claim 3 is a step of applying a water-repellent varnish to the lower surface of the A member, a step of drilling an inspection hole in the A member, and affixing the membrane filter to the inspection hole, a sample of the B member A step of punching and removing a portion to be a flow path and a sample storage portion, a step of pasting the C member and a D member in advance, a step of pasting an E member, and perforating an air hole; 3. The analyte analysis according to claim 2, comprising at least a step of bonding and integrating the members B, C, D, and E, punching a positioning hole, and punching the outer shape into a predetermined chip shape. It is the manufacturing method of the chip | tip.

  The analyte analysis chip according to the present invention has an elongated flat plate shape in which a plurality of sheet members are bonded, and the end opposite to the distal end to be inserted into the measuring device is a gripping portion. Since the structure has a sample introduction hole for introducing the analyte sample between the head and the gripper, the analysis operation can be performed without touching the analyte when introducing the analyte sample to the chip. it can.

  In addition, the sample introduction hole protrudes from the side surface of the chip, and since the water repellent treatment is applied to the periphery of the sample introduction hole, there is a problem that the sample adheres to the periphery of the sample introduction hole and contaminates the measurement device. Less likely to occur. For this reason, it is possible to perform the analysis process safely and cleanly even in hospitals, inspection institutions, and the like that analyze blood of unspecified persons.

  Further, the analyte analysis chip according to the present invention is configured by laminating a plurality of sheet-like members, and each member can be provided with necessary functions in a distributed manner. This is possible, and the material cost can be reduced.

  According to the manufacturing method of the third aspect, since all the steps can be mechanized without manpower, a stable quality analysis chip can be supplied quickly and in large quantities.

It is explanatory drawing which showed one embodiment of the chip | tip for analyte analysis based on this invention, (1) shows the state seen from the surface side, (2) shows the state seen from the back surface side. FIGS. 2A and 2B are cross-sectional explanatory views schematically showing a cross-sectional configuration of the analyte analyzing chip shown in FIG. 1, wherein FIG. 1A is a cross-sectional view taken along line XX in FIG. 1A, and FIG. A cross section is shown. FIG. 2 is an explanatory cross-sectional view schematically showing a layer configuration of the analyte analysis chip shown in FIG. 1. FIG. 2 is an enlarged view of a sample introduction hole portion of the analyte analysis chip shown in FIG. 1. FIG. 2 is a perspective view showing a layer configuration of the analyte analyzing chip shown in FIG. 1.

Hereinafter, the analyte analysis chip according to the present invention will be described in detail with reference to the drawings. FIG. 1 is an explanatory view showing an embodiment of the analyte analyzing chip according to the present invention. FIG. 1 (1) shows a state seen from the front side, and FIG. 1 (2) shows a state seen from the back side. FIG. 2 is an explanatory cross-sectional view schematically showing a cross-sectional configuration of the analyte analyzing chip shown in FIG. 2 (1) shows the XX section of FIG. 1 (1), and FIG. 2 (2) shows the YY section of FIG. 1 (1).
An analyte analysis chip 1 according to the present invention is an elongated flat plate analysis chip for analyzing a liquid analyte sample, and is formed by bonding a plurality of sheet members A to E.

The tip end portion 2 has a positioning hole 4 for positioning when inserted into the measuring device, and a sample introduction hole 6 for introducing a sample to be tested into the chip is provided on the side surface of the center portion of the chip. Have. The sample introduction hole 6 protrudes from the side surface of the chip.
Inside the chip, there are a sample reservoir 8, a sample flow path 7 for guiding the analyte sample introduced from the sample introduction hole 6 to the sample reservoir 8 by capillary action, and an analyte sample introduced into the sample reservoir 8. A membrane filter 71 to be processed is included.
The lower surface of the chip has an inspection hole 5 for taking out and inspecting the test liquid processed by the membrane filter 71, and the upper surface of the chip has a sample sample 7 for smoothly entering the sample channel 7. The air hole 9 is an air vent.

  The air holes 9 function to release the air inside the chip, where there is no escape space, to the upper surface side of the chip as the specimen sample flows along the sample flow path 7. The air hole 9 is opened at a portion where the sample channel 7 extends through the sample storage portion 8. For this reason, the subject sample that has entered from the sample introduction hole 6 proceeds through the sample flow path 7, fills the sample storage portion 8, and further reaches the air hole 9.

  The opposite end portion of the tip end portion 2 forms a grip portion 3 for gripping the tip, and a water repellent treatment in which at least the peripheral portion of the sample introduction hole 6 on the front surface and / or back surface of the tip is subjected to a water repellent treatment. The unit 10 is provided.

  The analyte analysis chip 1 according to the present invention is a chip used for analyzing a liquid analyte sample such as blood, urine, etc., and has a tip holding portion 3 and a sample introduction hole in the analyte. 6 is brought into contact, and the analyte is introduced along the sample flow path 7 by capillary action. At this time, since the air hole 9 for venting air is provided in the innermost part of the sample channel 7, the subject is smoothly introduced. The introduced analyte is collected in the sample storage unit 8 and comes into contact with the membrane filter 71.

  The membrane filter 71 is a membrane that has a function of separating only the measurement target component in the specimen sample. For example, if the subject is blood, a blood cell separation membrane that separates only plasma components from the blood is used. The test liquid that has passed through the membrane filter 71 is taken out from the test hole 5 and analyzed by a measuring device.

  In the analyte analyzing chip 1 according to the present invention, the grip 3 is provided at the end opposite to the tip 2 inserted into the measuring apparatus, and the sample introduction hole 6 is disposed in the middle thereof. Analysis operations can be performed without touching. Further, since the sample introduction hole 6 protrudes from the side surface of the chip, it is easy to prevent the subject from unnecessarily adhering to a portion other than the sample introduction hole when the sample introduction hole 6 is brought into contact with the subject. Can be. Further, since water repellent treatment is applied to at least the peripheral portion of the sample introduction hole 6 on the front surface and / or back surface of the chip, it is possible to prevent the subject from adhering to the periphery of the sample introduction hole 6 and contaminating the measurement device or the like. Has the effect of preventing. The water-repellent treatment is preferably performed on the back surface and the surface around the sample introduction hole of the analysis chip, but even if applied to either one of the surfaces, there is a sufficient effect. Since the portion of the inspection hole 5 that needs to be inserted into the measuring device is located between the sample introduction hole 6 and the tip end portion 2, the portion of the sample introduction hole 6 is inserted into the measuring device. There is no need. For this reason, even if the periphery of the sample introduction hole is contaminated by the subject, the inside of the measurement apparatus is not contaminated.

Examples of the water repellent treatment include a method of applying or printing a water repellent varnish, and a method of attaching a water repellent film. A method of applying a water-repellent varnish only to a necessary portion by a printing method has the least waste of material and high production efficiency.
FIG. 4 is an enlarged view of a sample introduction hole portion of the analyte analyzing chip shown in FIG.
In this embodiment, since the position of the sample introduction hole 6 is close to the back surface side of the chip, the water repellent treatment part 10 is provided only on the back surface side of the chip, but the above water repellent effect is sufficiently exhibited. Is done. In this embodiment, the reason why the water-repellent treatment part 10 is provided only on the back surface is that the A member used on the back surface is a material subjected to hydrophilic treatment on both surfaces.

  The analyte sample that has contacted the sample introduction hole 6 enters along the sample channel 7 by capillary action, but the inner wall of the sample channel 7 is wetted by the analyte sample in order to make the analyte sample enter smoothly. It is effective to make it easy. If the subject is blood or urine, this purpose can be achieved by making the inner wall of the sample channel 7 a hydrophilic surface. At this time, it is not always necessary to make the entire surface of the inner wall of the sample flow path hydrophilic. For example, a sufficient effect can be obtained only by making only the floor surface and the ceiling surface hydrophilic.

Next, the layer configuration and constituent materials of the analyte analysis chip according to the present invention will be described.
FIG. 3 is an explanatory cross-sectional view schematically showing the layer structure of the analyte analyzing chip shown in FIG. The analyte analysis chip according to the present invention is formed by bonding a plurality of sheet members. In the embodiment shown in FIG. 3, it consists of five sheet members A to E and a membrane filter.

  The A member (A) is a hydrophilic film 11 which is transparent and has a hydrophilic surface. An inspection hole 5 is drilled in the A member. The A member constitutes the floor surface of the sample channel 7. As materials used as the A member, hydrophilic treatment films such as hydrophilic treatment PET (polyethylene terephthalate resin) film, hydrophilic treatment acrylic resin film, hydrophilic treatment acetate resin film, etc. having a hydrophilic treatment on the surface, or the film itself is hydrophilic. A polyvinyl alcohol film or the like having, is not particularly limited. The degree of hydrophilicity may be anything as long as the contact angle when dripping water is 30 ° or less. In general, many of these commercially available materials have been subjected to hydrophilic treatment on both sides, but may be those in which only the surface in contact with the sample flow path 7 has been subjected to hydrophilic treatment. The reason why the member A is transparent is to allow the specimen sample such as blood to be seen in the sample channel 7.

  On the lower surface of the A member, that is, the surface serving as the bottom surface of the analysis chip 1, a water repellent treatment portion 10 is provided in which water repellent treatment is performed on at least a portion around the sample introduction hole 6. The reason for providing the water repellent treatment part is to prevent the sample from adhering to the chip surface when the sample introduction hole is brought into contact with the sample to be examined, as already described. As a method of forming the water repellent treatment portion 10, there are a method of applying a water repellent pressure-sensitive adhesive tape and a method of applying a water repellent varnish, but only a necessary portion using a screen printing method or a gravure printing method. A method of printing a water-repellent varnish is most preferable. As the degree of water repellent treatment, it is preferable that the contact angle when water is poured is 100 ° or more.

  The timing at which the water repellent treatment is applied to the A member is not particularly limited, and it may be pasted with another member to form a chip for analysis, but before being pasted with another member. The water repellent treatment may be performed on the lower surface of the A member in advance.

  The membrane filter 71 is affixed to the A member so as to cover the inspection hole 5 drilled in the A member. Therefore, the expensive membrane filter 71 need only be large enough to cover the inspection hole 5.

  The B member (B) is a member having the adhesive layers 22 and 23 on both surfaces of the base material 21, and the portions to be the sample flow path 7 and the sample storage portion 8 are punched and removed. By joining the B member so as to be sandwiched between the A member and the C member described below, the sample flow path 7 and the sample storage portion 8 are formed as a continuous space. As the B member, for example, a PET film-based double-sided pressure-sensitive adhesive tape or a material in which a double-sided pressure-sensitive adhesive tape is bonded to both surfaces of the PET film can be used. Since the punched side surface of the B member constitutes the wall surface of the sample channel 7, the B member needs to have a certain thickness and is desirably hydrophilic, but is not necessarily hydrophilic. In addition, since the punched portion becomes the sample channel 7, the B member does not need to be transparent, but rather is colored black or the like so that the portion of the sample channel 7 is clearly distinguished from the others. May be. In this case, a colored base material may be used as the base material 21. The B member is handled in a state in which a release paper or a release film is attached to both surfaces in a process in the middle of punching the sample flow path or the like.

  The C member (C) is a hydrophilic film 31 which is transparent and has a hydrophilic surface, like the A member. Although the same material as said A member can be used as C member, it is not necessary to perform water-repellent treatment. The C member constitutes the ceiling surface of the sample channel 7.

  Air holes 9 are perforated in the C member. The air holes 9 are also present at the same positions of the tips of the D member and E member, which will be described later, and air that does not escape as the analyte sample flows along the sample flow path 7 flows to the chip surface side. Work to escape.

  The D member (D) serves as a substrate for giving rigidity to the entire analysis chip 1. As the base material 41 of the D member, a transparent plastic sheet having a high rigidity of about 100 to 200 μm is suitable. As a material, general materials such as polypropylene resin, polyvinyl chloride resin, polystyrene resin, PET resin, acrylic resin and the like can be used. A heat sealable material layer 42 is provided on the upper surface of the substrate. The heat-sealable material layer 42 is a material that melts and adheres by heating, and a known heat-seal varnish, hot-melt adhesive, heat-sealable resin, heat-sealable film, or the like can be used. The heat sealable material layer 42 is formed by a method such as a gravure printing method, an extrusion laminating method, or a dry laminating method depending on the material. An air hole 9 is drilled in the D member at the same position as the C member.

  The E member (E) is a member that forms the uppermost surface of the analysis chip 1. The base material 51 of the E member is required to be a transparent material with good dimensional accuracy in order to print and form the printed layer 52, which is information necessary for use as an analysis chip, on the back surface. As the base material 51 of the E member, the same material as that used as the base material 41 of the D member can be used.

  Information displayed by the print layer 52 includes the name and model number of the chip, the display indicating the position of the gripping part and the insertion direction of the chip, the display indicating the position of the sample introduction hole, the end point position where the specimen sample should be introduced, etc. Information necessary for using the chip is displayed.

  As a method for forming the printing layer 52, a known printing method such as an offset printing method, a gravure printing method, a screen printing method, or an ink jet printing method is used. On the back surface of the E member, the same heat-sealable material layer 53 as that provided on the D member is formed on the printing layer 52. In the E member, air holes 9 are formed at the same positions as the C member and the D member.

  The analyte analysis chip according to the present invention is formed by laminating the A member, the membrane filter, and the B to E members in this order from the bottom, and then the positioning hole 4 is drilled. FIG. 5 is a perspective view showing the layer structure of the analyte analyzing chip shown in FIG. Hereinafter, the method for manufacturing the analyte analyzing chip according to the present invention will be described with reference to FIGS.

  First, a water-repellent varnish is applied to the lower surface of the A member to form the water-repellent treatment portion 10. The water repellent treatment unit 10 may be provided only in a necessary portion using a gravure printing method, a screen printing method, or the like. Next, the inspection hole 5 is formed in the A member, and the membrane filter 71 is attached to the upper surface side of the inspection hole 5 by an adhesive or heat seal.

  Next, the portion of the B member that should become the sample flow path 7 and the sample storage portion 8 is punched and removed. The punching operation can be efficiently performed using a punching die while continuously and sequentially feeding the laminate sheets to be the B member. In addition, although it has abbreviate | omitted in the figure after the punching process to the bonding process demonstrated later, it is set as the state which the release paper and the release film adhered to both surfaces of B member.

  Next, after the heat-sealable material layer 42 is formed on the surface of the base material 41 of the D member, it is bonded to the C member. For laminating, a dry laminating method using an adhesive is suitable. As shown in FIG. 3, the hydrophilic film 31 as the C member and the base material 41 of the D member are bonded by the dry laminate adhesive layer 61. (Hereinafter, this is referred to as a CD member. The same applies hereinafter.)

  Next, the printing layer 52 is formed on the back surface of the base member 51 of the E member, and the heat-sealable material layer 53 made of the same material as that used for the D member is formed on the printing layer 52 surface. Next, the heat-sealable material layer 53 and the heat-sealable material layer 42 of the CD member are opposed to each other, and the whole is heat-laminated and integrated. The heat seal strength at this time is preferably 1 N / 15 mm or more. The air holes 9 are perforated at a predetermined position of the bonding member (CDE member) formed as one sheet by the above.

  The release paper on the front and back surfaces of the B member is removed, and the A member, the B member, and the CDE member are bonded and integrated. Next, the positioning hole 4 is drilled, and the outer shape is punched into a predetermined chip shape by a punching die. The analyte analysis chip 1 is completed through the above steps.

Each of the above steps is a content that can be automatically performed by mechanical equipment without human intervention. Therefore, according to the manufacturing method including the above steps, the analyte analysis chip according to the present invention is provided. Can be manufactured very efficiently.
The analyte analysis chip according to the present invention will be described in more detail below based on examples.

After printing a predetermined printed pattern on the treated surface of a 188 μm thick PET resin film (S10 single-sided product manufactured by Toray Industries Inc.) by gravure printing, an EVA resin hot melt adhesive (DX-11C manufactured by DIC) is further added. 25 g / m ^{2 was} applied and slit to a width of 115 mm to obtain an E member.

A hot melt adhesive (DX-11C manufactured by DIC) is applied to the treated surface of a 188 μm thick PET resin film (S10 single-side processed product manufactured by Toray Industries, Inc.), and an adhesive for dry lamination (manufactured by Toyo Morton Co., Ltd.) is applied to the opposite surface. Urethane adhesive TM242A / B) was applied at 4 g / m ² and bonded to a 100 μm-thick PET resin film (S10 manufactured by Toray Industries, Inc.) subjected to hydrophilic treatment. This was aged in an atmosphere of 40 ° C. for 2 days and then slit to a width of 115 mm to obtain a CD member.

  A water-repellent varnish (PANNECO medium, manufactured by Toyo Ink Manufacturing Co., Ltd.) is printed on one side of a 100 μm-thick PET resin film (S10, manufactured by Toray Industries, Inc.) that has been subjected to a hydrophilic treatment, and is slit in a 115 mm width by a gravure printing method. A member was obtained.

The sheet member obtained as described above was set in a chip assembling apparatus, and the analyte analysis chip 1 shown in FIG. 1 was obtained according to the following steps.
1. An inspection hole having a diameter of 6 mm is formed in the A member, and a membrane filter (PORAFIL PC manufactured by MACHEREY-NAGEL, thickness 7 μm) is heat-sealed and pasted so as to cover the hole.
2. The CD member and the E member are heat sealed and bonded to form a CDE member, and then an air hole having a diameter of 0.5 mm is formed. The heat seal strength at this time was 1 N / 15 mm or more.
3. A 125 μm thick black PET film (B100 manufactured by Mitsubishi Plastics, Inc., B100) and a double-sided adhesive tape (double-sided tape No. 5620BW manufactured by Nitto Denko Corporation) on a sheet with a total thickness of 200 μm B shape is obtained by punching the shape.
4. After bonding the A member and the CDE member via the B member, a positioning hole having a diameter of 2 mm is formed.
5. It is punched into a predetermined outer shape having a width of about 10 mm and a length of about 75 mm to obtain a specimen inspection chip 1 as shown in FIG.

  The analyte analysis chip obtained according to the above steps was used as a blood glucose measurement chip, and the quality was stabilized and the productivity was greatly improved as compared with a product assembled by a conventional manual operation.

DESCRIPTION OF SYMBOLS 1 ... Analytical analysis chip 2 ... Chip tip 3 ... Holding part 4 ... Positioning hole 5 ... Inspection hole 6 ... Sample introduction hole 7 ... Sample flow path 8 ..Sample reservoir 9 ... Air hole 10 ... Water repellent treatment part A ... A member B ... B member C ... C member D ... D member E ... E member 11 , 31 ... hydrophilic films 21, 41, 51 ... base material 22, 23 ... adhesive layer 42, 53 ... heat sealable material layer 52 ... printed layer 61 ... dry laminate adhesion Agent layer 71 ... Membrane filter

Claims (3)

It is an elongated flat plate analysis chip for analyzing a liquid specimen, and a plurality of sheet members are bonded together,
The tip of the tip has a positioning hole for positioning when inserted into the measuring device,
On the side surface of the center of the chip, there is a sample introduction hole protruding from the side surface of the chip for introducing the specimen sample into the chip,
Inside the chip, there are a sample reservoir, a sample channel for guiding the analyte sample introduced from the sample introduction hole to the sample reservoir by capillary action, and a membrane filter for processing the analyte sample introduced into the sample reservoir. Have
On the lower surface of the chip, there is an inspection hole for taking out and inspecting the processed test liquid,
The top surface of the chip has an air hole for smooth entry of the specimen sample into the sample flow path,
The opposite end of the tip of the tip forms a grip for gripping the tip,
An analyte analyzing chip, wherein a water repellent treatment is applied to at least a portion around the sample introduction hole on the front surface and / or back surface of the chip. The plurality of sheet members are:
A member which is transparent and has a hydrophilic surface and a perforated inspection hole;
A membrane filter,
B member having an adhesive layer on both sides of the substrate and punched and removed the portion to be the sample flow path and the sample reservoir;
A transparent C member having a hydrophilic surface and perforated air holes;
D member which is transparent and has a heat-sealable material layer on its upper surface and has air holes drilled;
At least an E member that is transparent and has a printed layer and a heat-sealable material layer on the lower surface and has air holes drilled therein,
2. The analyte analyzing chip according to claim 1, wherein the A member, the membrane filter, and the B to E members are laminated in this order from the bottom, and a positioning hole is perforated.   A step of applying a water-repellent varnish to the lower surface of the A member, a step of drilling an inspection hole in the A member, and affixing the membrane filter to the inspection hole; The step of punching and removing, bonding the C member and D member in advance, and further bonding to the E member to punch air holes, and the A member, B member, C, D, and E member having undergone the above step 3. The method for producing an analyte analyzing chip according to claim 2, comprising at least a step of pasting and integrating, punching a positioning hole, and punching an outer shape into a predetermined chip shape.

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