JP2012247231A - Inspection instrument - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide means for accurately, efficiently and safely performing the apply operation of a detection object collected from a specimen to a matrix for detection.SOLUTION: Disclosed is an inspection instrument including (1) a fluid storage part for storing a fluid for extracting a specimen accompanied with an opening section at a front part and a small hole at a bottom part, (2) a first sealing member for sealing the opening section of the fluid storage part and a second sealing member for sealing the small hole at the bottom part, (3) a matrix for detection, and (4) one or more detection windows for visualizing the detection signal of the matrix for detection from the outside. The inspection instrument is configured such that the small hole at the bottom part of the fluid storage part and the matrix for detection are held so as to be interposed with the second sealing member, and that the second sealing member is formed so as to be directly or indirectly projected to the outside of the inspection instrument, and that when a tensile force is applied to the projecting section, the sealing section of the small hole in the second sealing member is peeled, and the matrix for detection and the small hole at the bottom part of the liquid storage part are directly brought into contact with each other. Disclosed is a method of using the inspection instrument.

Description

  The present invention relates to a test instrument for detecting a measurement object in a sample in immunoassay, tumor marker measurement, and the like, and a method of using the instrument.

  As a conventional inspection instrument, after preparing a sample solution from which a measurement object is extracted, the sample liquid is transferred to a detection unit through a detection matrix such as a nonwoven fabric, and then a measurement object is obtained using a signal such as an antigen-antibody reaction. Those that perform detection are known.

  The problem proposed in the present invention is an operation of applying a detection object collected from a specimen to a detection matrix. Examples of prior art that suggests this problem include the following.

  Patent Document 1 discloses a specimen collection container that is separate from the detection instrument for appropriately applying the specimen liquid to the detection instrument. Patent Document 2 discloses an allergen test kit that extracts allergens collected by a vacuum cleaner with a liquid contained in a device and detects a signal in the extract.

JP 2010-38797 A JP-A-11-248705

  As described above, an object of the present invention is to provide means for reliably, efficiently and safely applying an application of a detection object collected from a specimen to a detection matrix.

  For example, there is a case where a simple inspection is performed at the bedside, and there are some problems that are desired in an inspection instrument used at that time. When performing a simple test, it may be desirable to minimize the amount of sample collected in consideration of pain to the sample provider. Therefore, it is necessary to use the obtained specimen for examination as efficiently as possible. Further, when testing a plurality of samples, it is necessary to prevent sample mix-up. For a quicker inspection, it is necessary to prevent contamination and improve the accuracy of the inspection, and to perform the inspection with the simplest procedure possible.

  The sample collection container of Patent Document 1 is a container separate from the detection instrument, and it takes time and effort to attach and detach the detection instrument to and from the container, and the risk of the sample extract leaking out in the process Is considered to exist. Moreover, in the allergen test kit of patent document 2, since the detection target is an allergen (house dust), a suction operation by a vacuum cleaner is premised, and this is used to actively extract a specimen using the configuration of the disclosed technology. It is difficult to apply to infectious disease inspections that require processes.

  The present inventor solves the above problem by integrating a field for extracting a collected specimen and a field for developing the extract by chromatography, and performing a series of detection operations with a single detection instrument. As a result, the present invention was completed. In the present invention, the sample extraction operation can be performed in the detection instrument, and the sample extraction solution that has undergone the extraction operation can be applied to the detection matrix in one step.

  That is, firstly, the present invention includes (1) a liquid storage section containing a liquid for extracting a specimen, with an opening portion at the front portion and a small hole at the bottom portion, and (2) an opening of the liquid storage portion. A first sealing member that can be peeled or broken by an external force that seals the portion, a second sealing member that can be peeled by an external force that seals the bottom small hole, and (3) the sample extracted A detection matrix provided with a moving part for moving the liquid by the infiltration force and a detecting part for detecting the detection object, and (4) one or more detections for visualizing the detecting part from the outside An inspection instrument comprising a window, wherein the bottom small hole of the liquid storage portion and the moving portion of the detection matrix are kept sandwiched between the second sealing members, and the second sealing member is Project directly or indirectly to the outside of the detection device and pull it By applying a tension force, the sealing portion of the small hole in the second sealing member is peeled off, and the detection matrix and the bottom small hole of the liquid storage unit are in direct contact with each other (hereinafter, the present invention). (Also referred to as an inspection instrument).

  In the inspection instrument of the present invention, the moving portion of the detection matrix has an area portion wider than the cross-sectional area of the bottom small hole of the liquid storage portion, and the second sealing is performed by the wide area portion and the bottom small hole. In order to prevent waste of the sample extract, it is preferable that the member is sandwiched.

  In addition, leakage of the sample extract is that the pressing portion by the pressing member is applied to the sandwiched portion by the bottom small hole of the liquid storage portion, the second sealing member, and the moving portion of the detection matrix. It is suitable for preventing the above. Further, the liquid absorption infiltrating member is sandwiched at a position corresponding to the lower part of the bottom small hole of the liquid storage part between the second sealing member and the detection matrix, thereby preventing the specimen extract from leaking out. It is suitable for carrying out the process more completely.

  Moreover, the sealing part with respect to the bottom small hole of the liquid storage part in the second sealing member is an end part that is folded on the opposite side in the length direction from the protruding part of the container of the second sealing member. By applying a pulling force to the projecting portion, the folded state of the opposite side end is released, and the bottom small hole and the detection matrix are in direct contact with each other from the small hole of the second sealing member. This is suitable for smooth peeling.

  Furthermore, it is possible and preferable to provide a place on the inner wall surface of the liquid storage portion where the attached portion (for example, the head of a cotton swab) of the collection target such as a cotton swab can be scraped off. .

  It is also possible to provide means for promoting the movement of moisture in the matrix. Specifically, providing a transpiration window on the downstream side of the detection matrix with respect to the detection window is exemplified.

2ndly, this invention is invention which provides the usage method (henceforth the usage method of this invention) of the test | inspection instrument of this invention containing following step (1)-(3).
(1) One end of the first sealing member of the liquid container is peeled off or broken, and the tip of the collection rod to which the sample is attached is inserted into the liquid container and brought into contact with the liquid in the part A step of extracting the sample in the liquid,
(2) A tensile force is applied to the projecting portion of the second sealing member to the outside, and the sealing member is peeled off from the small hole so that the in-liquid extract in the liquid storage portion is moved to the moving portion of the detection matrix. Contacting step,
(3) Confirm through the detection window the signal that appears due to the contact reaction that accompanies the movement of the liquid detection matrix from the moving part toward the detecting part due to the extract in the liquid coming into contact with the detecting matrix. And detecting the measurement object.

  The inspection instrument according to the present invention includes, as an accessory, a cover member for sealing the opening portion in the front portion of the liquid storage portion after the first sealing member is peeled or broken, during the inspection operation. It is possible to thoroughly prevent contamination of the surrounding environment due to the scattering of the extract in the liquid. That is, in the above-described method of using the present invention, the step [(2) ′] of sealing the opening portion in the front portion of the liquid storage portion with the lid member is performed after the extraction of the specimen in the liquid in step (1). Thus, when performing the following steps (2) and (3), it is possible to thoroughly prevent contamination of the surrounding environment due to scattering from the opening portion of the liquid container. The lid member is usually provided as a “substantially independent accessory”, which means that the lid member is substantially independent of the body portion of the test instrument, eg, a test A case where the instrument main body and the lid member are paired with a string or the like is also included in this “substantially independent”. In addition, it is also possible to take a form in which the front opening portion is sealed by the lid member in a detachable form from the beginning, and is removed once at the start of use and re-sealed when necessary. Independence ".

  The above-mentioned “integration of the field of extraction of the collected specimen and the field of development of the extract by chromatography” is performed by peeling the second sealing member by an external pulling force. An in-liquid extract obtained by extraction of a sample in a liquid container provided in the inspection instrument is brought into contact with a moving part of a detection matrix placed thereunder through a small hole at the bottom of the liquid container. Is realized. That is, in one test instrument, “extraction of the specimen in the liquid in the liquid storage part → detachment of the second sealing member and contact of the detection matrix with the liquid → movement of the specimen liquid upstream of the matrix → detection in the detection zone” By performing a series of steps of “signal confirmation”, the above-mentioned “field integration” is realized, and this problem can be solved. The method of use of the present invention defines the above series of steps.

  The present invention is applicable to any target in the fields of clinical testing, food testing, environmental testing, crime investigation, etc., as long as it is a target that can be detected based on an antigen-antibody reaction. In addition, for example, a method using a gene amplification product of a specific biological species (bacteria, virus, etc.) by a PCR method as a specimen and using a hybridization between nucleic acids as a detection principle is also possible.

  According to the present invention, a specimen inspection instrument capable of performing a series of detection operations with a single detection instrument by integrating a field for extraction of a collected specimen and a place for development by chromatography of the extract, A method of use is provided. The test instrument of the present invention is easy to handle and operate, and in particular, specimen testing by immunochromatography can be performed simply, reliably and rapidly. In addition, it is possible to appropriately prevent contamination of the surrounding environment.

It is the exploded view seen from the side surface of the length direction of the inspection instrument of the present invention. It is a top view of the 1st sealing member of the inspection instrument of the present invention. It is a top view of the 2nd sealing member of the inspection instrument of the present invention. It is the whole inspection instrument perspective view of the present invention. It is sectional drawing seen from the side surface direction of the length direction which showed the usage method of this invention.

  An embodiment of the present invention will be described with reference to FIGS. The form shown here is an example, and it is possible to make a design change as necessary within the scope of the present invention. Of course, an aspect in which the design change is made is also within the scope of the present invention. It is.

  FIG. 1 is an exploded view seen from the side surface in the length direction of the inspection instrument 1 of the present invention, and FIG. 3 is an overall perspective view of the inspection instrument 1. Components constituting the inspection instrument 1 are a lower casing member 10, a spring 11, a detection matrix 20, a liquid-absorbing infiltrating member 30, a second sealing member 40, an upper casing member 50, and a first sealing. This is the member 60. The lower housing member 10 and the upper housing member 50 have a structure that can be engaged and fixed to each other.

  The lower housing member 10 is provided with a field (not shown) in which the spring 11 and the detection matrix 20 can be placed and fixed. The field is formed, for example, by providing a continuous or non-continuous convex structure in the lower housing member 10 that forms a space to be fitted when the detection matrix 20 is placed. The placement of the spring 11 and the detection matrix 20 in the field may or may not be accompanied as long as the placed position is fixed. The upper housing member 50 is provided with a liquid storage portion 51 that contains an opening 511 in the front portion and a small hole 512 in the bottom portion, and stores a liquid 514 for extracting a specimen. Further, a field 513 that can scrape off the portion of the collection rod 2 to which the detection target is attached is provided on the inner wall surface of the liquid storage unit 51. The upper casing member 50 has a thick structure in the vicinity of the liquid storage portion 51 in order to secure the inner volume, and conversely, the vicinity of the detection window 52 makes the detection matrix 20 more easily visible. In addition, the portion has a concave structure with a taper. As long as one or more detection windows 52 can visually detect the detection part 22 of the detection matrix, the window part may be accompanied by a transparent member such as transparent glass or transparent plastic, or anything. It is not necessary. Furthermore, a slide hole that allows the slide portion 44 of the second sealing member 40 to be pulled from the outside at the center end portion of the engagement portion of the upper housing member 50 with the lower housing member 10 on the thick structure side. 53 is provided. The slide hole 53 is wider than the width of the slide portion 44 in the thickness direction, and is thicker than the thickness of the second sealing member 40. Such a slide hole 53 can be provided on the lower housing member 10 side, for example, or can be provided so as to be engaged with both the lower housing member 10 and the upper housing member 50. .

  The detection matrix is made of a material exhibiting a capillary action, and examples thereof include non-woven fabric, nitrocellulose, cellulose, and glass fiber. Moreover, even if it is a single material, it may be formed by bonding a plurality of materials. The detection matrix 20 is a typical example, and the detection unit 22, the moving unit 23, and the absorption unit 24 include one end of the detection unit 22 and the other end of the movement unit 23, and the other end of the detection unit 22 and the absorption unit 24. They are fixed in contact with each other. This fixing can be done by adhering each part in a water-permeable manner, but placing the adhesive tape on the bottom and fixing each part sequentially in the length direction by the adhesive force of the tape Thus, the detection matrix 20 can be created completely. If the liquid comes into contact with one end of the moving unit 23, the moving unit 23, the detecting unit 22, and the absorbing unit 24 move in order due to capillary action. In the process, the detection unit 22 detects a specific component in the liquid. In order to prevent waste of the specimen extract, it is preferable that at least the moving unit 22 has an area portion wider than the cross-sectional area of the small hole 512.

  The spring 11 disposed between the detection matrix 20 and the lower housing member 10 is a “pressing member” used as a selective constituent element in the detection instrument of the present invention, and is positioned on the detection matrix 20 and the upper part thereof. Any other structure may be used as long as it is a member that applies a pressing force to the lower entrance of the bottom small hole 512 of the liquid storage unit 51. For example, elastic rubber or sponge can be used. The spring 11 can improve the leakage preventing performance of the specimen extract derived from the liquid storage unit 51 by the pressing force.

  FIG. 2 shows respective plan views of the sheet-like first sealing member 60 (FIG. 2-1) and the second sealing member 40 (FIG. 2-2). The first sealing member 60 is a sheet-like member for sealing the opening portion 511 of the liquid storage unit 51. The one end 61 has a pointed shape that is slightly larger than the shape in accordance with the shape of the opening 511, and the other end 62 is a peeling start portion so that it can be easily peeled off by hand. The size has room. A dotted line 63 indicates a fold line or a cut line that can be selectively adopted. Specifically, the means for facilitating the bending or cutting of the dotted line portion, that is, perforations, etc. are applied, so that after extraction in the liquid of step (1) in the method of use of the present invention described later, When the peeled first sealing member 60 is returned to the original sealed state again for the purpose of preventing the extract in the liquid from scattering, the dotted line portion 63 can be easily folded or cut off. Bending or cutting is performed so that the detection window 52 can be visually observed without obstructing the field of view by the 62 portion of the first sealing member 60. When the dotted line is a cut line, the cut line is easily maintained without being broken even if the other end 62 portion is pulled to apply a pulling force when the first sealing member 60 is peeled off. A certain degree of strength is required. Further, when the first sealing member 60 is broken by the cotton swab 2 without pulling, the positive necessity of the 62 portion itself is not recognized. Further, as will be described later, after the extraction in the liquid in the step (1), the lid member 70 capable of sealing the opening portion 511 in the front portion of the liquid storage portion 51 is engaged with the opening portion. In addition, it is possible to prevent scattering of the extract in the liquid and facilitate visual inspection of the detection window 52.

  The vicinity of the one end 41 of the second sealing member 40 is a region for covering the small hole 512 at the bottom of the upper housing member 50, and has a sufficient area that can cover the small hole as a preferable aspect. The other end 42 is wide so that it can be easily picked in an area to be pulled by hand. In this example, the second sealing member directly protrudes to the outside. For example, the vicinity of the other end 42 is changed to a part of the second sealing member 40 as in this example. Also, it is possible to adopt an “indirect projecting aspect to the outside” which is made of another member, for example, a shape that is easy to pull or a string member made of a material. Furthermore, a stopper region 43 wider than the slide hole 53 is provided on one end 41 side of the intermediate region, and a narrow slide region 44 is provided on the other end 42 side. One end 41 of the second sealing member 40 is folded into a state 41 ′, thereby forming a loop and contacting the lower small hole 512. In this example, the folding direction is directed toward the lower small hole 512. However, the folding direction may be performed toward the detection matrix 20 side. Both the first sealing member and the second sealing member, which are premised on peeling, are preferably made of a material that is thin and strong and that is excellent in waterproofness and durability. Examples of such a material include an aluminum / polyethylene laminate sheet, an aluminum sheet, and a plastic film. When it is assumed that the first sealing member is not peeled but is broken, it is necessary to use a material that can be easily broken by a pressing force. In view of practicality, it is preferable that the first sealing member is based on peeling as in this example.

  In this example, the liquid-absorbing infiltrating member 30 can be interposed at a position corresponding to the lower portion of the bottom small hole 512 of the liquid storage portion 51 between the second sealing member 40 and the detection matrix 20. This liquid infiltrating member 30 is preferably a material that efficiently infiltrates the moving portion 22 of the detection matrix 20 while absorbing and trapping the specimen extract from the lower small hole 512, for example, polyvinyl Examples thereof include a porous member based on alcohol, polyurethane and the like, and a fiber member based on cellulose, glass wool and the like. By using such a liquid-absorbing infiltrating member, the leakage of the specimen extract can be prevented more completely.

  Furthermore, in order to prevent the parts in the inspection instrument of the present invention described above from being disassembled after assembling, disassembly preventing means can be provided as necessary. For example, it is also possible to provide a locking mechanism (for example, a locking mechanism by a combination of a concave structure and a convex structure: not shown) that can lock the upper and lower housing members so as not to come off. . It is also possible to provide a transpiration window (not shown) at a portion of the upper housing member 50 corresponding to the absorption portion 24 of the detection matrix 20 to further promote the movement of the liquid in the detection matrix 20. Furthermore, a design device for facilitating the use of the present inspection instrument can be made as necessary. For example, the hollow shape with fine irregularities represented by 12 makes it easy to grasp the inspection instrument 1, for example, when the first sealing member 60 is peeled off or when the second sealing member 40 is pulled. It is a structure for preventing slipping.

  FIG. 4 is a cross-sectional explanatory view seen from the side surface in the length direction of the method of using the inspection instrument 1.

  FIG. 4 (1) shows the state shown in the overall perspective view of FIG. 3 before being used in a typical manner in the initial state 1 of the inspection instrument 1 of the present invention. A liquid 514 for extracting a specimen is stored in the liquid storage unit 51, and the liquid 514 and the liquid-absorbing infiltrating member 30 are separated from each other by the second sealing member 40 and cannot contact each other. FIG. 4 (2) shows a state immediately after the cotton swab 2 is inserted. At the time of this insertion, the first sealing member 60 of the liquid storage unit 51 has been peeled off, and the cotton swab 2 has been inserted. In this figure, the cotton swab 2 is inserted from the right hand direction, but the insertion direction is not particularly limited. For example, when the first sealing member 60 is peeled off and sealed again with the first sealing member 60 after the specimen extraction operation, the first sealing member 60 has its one end portion 61 removed. Only the other end 62 is peeled off, the cotton swab 2 is inserted from the left-hand direction, the sample extraction operation is completed, and the cotton swab 2 is pulled out. Then, the partially peeled portion is returned to the original state and re-adhered. It can be set as a reseal state. In this case, the adhesive used as the sealing means of the first sealing member 60 can exhibit a suitable adhesive force even after the first peeling, to the extent that it can be resealed. It is necessary. Further, when re-sealing with the first sealing member 60 as described above, the bending line or the cut line 63 is provided as described above, which hinders visual inspection of the detection window 52. It becomes easy to bend or remove the 62 parts. Further, as described above, in addition to the peeling shown here, the first sealing member 60 can be broken by the cotton swab 2 to perform substantially the same specimen extraction operation.

  By these operations, the liquid 514 for extracting the sample in the liquid storage unit 51 comes into contact with the head of the cotton swab 2, and the sample attached to the head of the cotton swab 2 is extracted in the liquid 514. The specimen to be extracted is not particularly limited. For example, if used for clinical laboratory use, blood (whole blood, serum, plasma, etc.), lymph, urine, saliva, nasal discharge, tears, feces, lung lavage fluid, Examples thereof include bone marrow fluid and the like, suspensions and lysates thereof, and the like. Moreover, if it uses for the test | inspection use of an environment or a foodstuff, the soil, water, foodstuffs etc. which become a test object, or these suspensions and melted substances are mentioned. Furthermore, the nucleic acid of specific biological species, such as bacteria and a virus, especially the gene amplification product obtained by PCR method etc. are mentioned. The liquid 514 is not limited as long as it is a solvent suitable for extracting these specimens, and examples thereof include water, aqueous solvents such as various buffer solutions, and organic solvents such as alcohol, acetone, and carbon tetrachloride. Is done. The concave-convex structure 513 is provided on the inner wall surface of the liquid storage unit 51, and by this, the sample attached to the head portion can be efficiently extracted by scraping the head of the swab 2 (arrow A). The position of the field and the specific structure for scraping such as the concavo-convex structure 513 are not particularly limited as long as the efficiency of scraping of the target specimen can be achieved. Further, a recess structure (not shown) for facilitating insertion of the cotton swab 2 can be provided in the vicinity of the bottom of the liquid storage portion 51. FIG. 4 (3) shows a stage in which the extraction of the sample is completed and the liquid-extracted swab 2 is extracted from the liquid container 51 in the direction of arrow B. The liquid 514 'is already in a state that can contain the detection target. 4 (1) to (3) above show “step (1)” of the detection method of the present invention.

  FIG. 4 (4) shows “Step (2) (including (2) ′) and Step (3)” of the detection method of the present invention. That is, the lid member 70 is fitted to the outer edge 515 of the opening portion in the front portion of the liquid storage portion 51 (arrow C), and the opening portion is resealed. This re-sealing by the lid member 70 is of course when all the first sealing member is peeled off as in this example, and when the first sealing member 60 as described above is present in the part. Can also be done. By pulling the other end 42 of the second sealing member 40 with a pulling force and releasing the loop including the one end 41 ′, the isolation state by the second sealing member 40 is released, and the lower small hole 512 and the liquid absorption infiltration are released. The member 30 is in direct contact, and the extracted liquid 514 ′ is impregnated in the member 30, and the liquid 514 ′ is applied to the moving part 23 of the detection matrix 20 in contact therewith (arrow E 1). Next, due to capillary action in the detection matrix 20, the liquid 514 ′ moves in the direction of the arrow E 2, and a signal that appears due to a contact reaction with the detection element carried by the detection unit 22 is confirmed through the detection window 52. Shows the detection stage of the measurement object. The signal is typically a signal that accompanies an antigen-antibody reaction related to a detection target, and is specific to, for example, a measurement target that is carried by the moving unit 23 and is movable along with the movement of moisture. In a state in which the detection target is bound to a labeled antibody carrying a colloidal gold antibody, for example, a colloidal gold, it is trapped by the second antibody specific to the measurement target immobilized on the detection unit 22, and this is detected in the detection zone. Since the colloidal gold accumulates in a visible state at 22, the object to be detected in the liquid 514 ′ can be measured. Further, the remaining liquid 514 ′ is absorbed into the absorption zone 24 as needed, so that the movement of moisture is maintained. The antigen-antibody reaction signal is not particularly limited, and examples thereof include color development and fluorescence using a chromogenic enzyme, a fluorescent dye, and the like. When using a nucleic acid hybridization reaction, a nucleic acid complementary to the nucleic acid to be detected is immobilized in the detection zone 22 instead of the labeled antibody, and the complementary nucleic acid is hybridized. Thus, by providing a means for emitting or quenching light, a desired nucleic acid can be measured.

  The above-described embodiment is one embodiment of the present invention, and other embodiments can be adopted as necessary.

  As described above, in the test instrument of the present invention, the extraction of the specimen into the liquid is performed in the structure provided in the instrument, and the contact between the extract and the detection matrix is made within the same instrument. This can be done in steps. That is, all the collected samples can be used, and the amount of collected samples can be minimized. In addition, since all operations can be performed in substantially the same instrument, it is possible to prevent sample mix-up when testing a plurality of samples. Furthermore, by re-sealing the liquid storage part, it becomes possible to thoroughly prevent contamination due to scattering of the specimen extract to the external environment. In addition, the inspection step is simplified as a whole, and a quick inspection is possible, and the reliability of the inspection is not impaired at all. Therefore, industrial applicability in the inspection instrument of the present invention and the method of using the inspection instrument is sufficiently recognized.

DESCRIPTION OF SYMBOLS 1 Test | inspection instrument 2 Cotton swab 10 Lower housing | casing member 11 Spring 20 Detection matrix 30 Liquid absorption infiltrating member 40 2nd sealing member 50 Upper housing | casing member 51 Liquid accommodating part 511 Opening part in the front part of a liquid accommodating part 512 Liquid accommodating Small hole 513 in the bottom of the part 513 A place where the attached portion of the detection target can be scraped 514 Liquid for extracting the specimen 52 Detection window 60 First sealing member 70 Lid member

Claims (10)

  (1) A liquid storage part containing a liquid for extracting a specimen, with an opening part in the front part and a small hole in the bottom part, and (2) peeling by an external force that seals the opening part of the liquid storage part or A first sealing member that can be broken, a second sealing member that can be peeled off by an external force that seals the bottom small hole, and (3) for moving the liquid from which the specimen has been extracted by infiltration force An inspection instrument comprising a detection matrix provided with a detection unit for detecting a moving unit and a detection object, and (4) one or more detection windows for visualizing the detection unit from the outside. The bottom small hole of the liquid storage part and the moving part of the detection matrix are kept sandwiched between the second sealing members, and the second sealing member is directly or indirectly placed outside the detection instrument. By projecting and applying a tensile force to the projecting part Said by sealing portions peeling of pores in the second sealing member, the bottom pores of the detection matrix and the liquid containing portion is in direct contact, the inspection instrument.   The moving part of the detection matrix has an area part wider than the cross-sectional area of the bottom small hole of the liquid storage part, and the second sealing member is sandwiched between the wide area part and the bottom small hole. Item 1. The inspection instrument according to Item 1.   The inspection according to claim 1 or 2, wherein the sandwiched portion by the bottom small hole of the liquid storage unit, the second sealing member, and the moving portion of the detection matrix is in a state where a pressing force is applied by the pressing member. Instruments.   The test according to any one of claims 1 to 3, wherein the liquid-absorbing infiltrating member is sandwiched at a position corresponding to a lower portion of the bottom small hole of the liquid storage portion between the second sealing member and the detection matrix. Instruments.   The sealing part for the bottom small hole of the liquid storage part in the second sealing member is an end part that is folded on the opposite side in the length direction from the protruding part in the container of the second sealing member, The inspection instrument according to any one of claims 1 to 4, wherein a folded state of the opposite end is released by applying a pulling force to the protruding portion, and the bottom small hole and the detection matrix are in direct contact.   The inspection instrument according to any one of claims 1 to 5, wherein a place capable of scraping off a portion to which the detection object is attached on the sampling rod is provided on the inner wall surface of the liquid storage unit.   The inspection instrument according to any one of claims 1 to 6, wherein a transpiration window is provided downstream of the detection matrix with respect to the detection window.   The inspection according to any one of claims 1 to 7, wherein a lid member capable of sealing the opening portion in the front portion of the liquid storage portion after peeling or breaking the first sealing member is provided as an accessory. Instruments. The usage method of the test | inspection instrument in any one of Claims 1-7 containing following step (1)-(3).
(1) One end of the first sealing member of the liquid container is peeled off or broken, and the tip of the collection rod to which the sample is attached is inserted into the liquid container and brought into contact with the liquid in the part A step of extracting the sample in the liquid,
(2) A tensile force is applied to the projecting portion of the second sealing member to the outside of the container so that the sealing member is peeled from the small hole, and the in-liquid extract in the liquid storage portion is moved to the detection matrix moving portion. Contacting the step,
(3) Confirm through the detection window the signal that appears due to the contact reaction that accompanies the movement of the liquid detection matrix from the moving part toward the detecting part due to the extract in the liquid coming into contact with the detecting matrix. And detecting the measurement object. The usage method of the test | inspection instrument of Claim 8 containing following step (1)-(4).
(1) One end of the first sealing member of the liquid container is peeled off or broken, and the tip of the collection rod to which the sample is attached is inserted into the liquid container and brought into contact with the liquid in the part A step of extracting the sample in the liquid,
(2) a step of sealing the opening portion in the front portion of the liquid storage portion by the lid member;
(3) A tensile force is applied to the projecting portion of the second sealing member to the outside of the container so that the sealing member is peeled from the small hole, and the in-liquid extract in the liquid storage portion is moved to the detection matrix moving portion. Contacting the step,
(4) Confirm through the detection window the signal that appears due to the contact reaction that accompanies the movement of the liquid detection matrix from the moving part to the detecting part due to the extract in the liquid coming into contact with the detecting matrix. And detecting the measurement object.

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