JP2011121958A - Mite structure, use thereof, method for breeding predatism fogging mite amblyseiusswirskii, breeding system for breeding the predatism fogging mite and biological insect pest control method for crops - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a biological insect pest control method in crops using a mite structure. <P>SOLUTION: As a new mite structure containing a population of a predatism fogging mite species Amblyseius Swirskii and an artificial host population which can be used to breed the fogging mite species or pasture the fogging mite species to the crops, the predatism fogging mite species Amblyseius Swirskii is artificially bred. Further, insect pest control for the crops is carried out using the mite structure. <P>COPYRIGHT: (C)2011,JPO&INPIT

Description

  The present invention, according to a first aspect, relates to a novel tick composition.

  According to a second aspect, the present invention relates to a novel method for rearing a predatory burdock mite species Amblyseius swirskii.

  According to a third aspect, the invention relates to a novel use of an artificial host mite (Astigmatid mite) for breeding the predatory burdock mite species Amblyseius swirskii.

  According to the fourth and fifth aspects, the present invention relates to a new breeding system for breeding the predatory burdock mite species Amblyseius swirskii and the use of this breeding system for the control of crop pests.

  According to a further aspect, the present invention relates to a method for controlling biological pests in crops, which utilizes the mite composition of the present invention.

  Predatory dust mites (Phytoseiidae) are widely used for biological control of spider mites and thrips in greenhouse crops. The most important thrips species in greenhouse crops are the Western Flower Thrips (Franklinella occidentalis) and the Onion Thrips (T. thrips). These include the predatory mites Amblyseius cucumeris and Amblyseius barkeri (Hansen, L.S. and Geyti, J., 1985; Ramakers, PM J. and van Lieburg, 198; Ramakers, P.M.J., 1989; Sampson, C., 1998; and Jacobson, R.J., 1995) and Iphiseius degenerans (Ramakers, P.M.J. and Voet, S.J.P.,). 1996). In the absence of prey, these tick species can settle and remain in crops that provide a continuous supply of pollen, such as capsicum (Capsicum annuum L.), eg cucumber and most These crops cannot be used in crops where pollen is not freely available, for example, if they are not artificially fed, this can be done by spraying the plant with pollen. .

  Alternatively, a controlled release system (such as disclosed by Sampson, C. (1998) or in British Patent No. 2,393,890) can be used for the red mite. This controlled release system consists of a sachet with a compartment containing a diet mixture consisting of straw, yeast and wheat germ, a population of cereal mites, Tyrophagus putrescentiae, and a predatory mite, Kukumeri kaburidani It consists of a group. The grain mite, Kenagakonadani, uses its dietary mixture to generate an active population and serves as an artificial host for the predatory tick population. The sachets are hung on the crop by hooks and will continuously release predatory mites over a period of 4 to 6 weeks.

  As a result, the number of predatory mites must be released to have sufficient pest control because the number of responses to the presence of food is rather weak. This is because the dietary mixture described above can be used to breed a sufficient amount of grain mites, Kenagakonadani mites, which can be used to economically breed very large numbers Economically possible.

  There are also more effective predatory ticks, such as Typhloromanus limonicus and Iphiseius degenerans, with higher predation rates and numbers of responses to thrips, but Kukumeri kaburani mites can easily grow in very large quantities, It is the most commonly used species.

  Iphiseius degenerans can be raised in large quantities using a castor bean plant (Ricinus communis L., Euphorbiaceae) that can provide a continuous supply of pollen and thereby allow mites to grow it can. Due to the large area required to grow these plants and the high investment in the greenhouse, the cost of Iphiseieus degenerans is very high compared to Kukumeriskaburid mites. Due to this high cost, breeders can release only a very small number, generally 1000 to 2000 predatory mites per hectare. Thus, the application of Iphiseieus degenerans is limited to chili (Capsicum annuum L.) that provides enough pollen that predatory mites can generate sufficient populations for pest control. The population of Iphiseius degenerans can take several months to be present in the crop with sufficient strength to be able to have a significant effect on the thrips pest population.

  The spider mite (Tetranychus urticae) has been successfully controlled in greenhouses and outdoor crops around the world by releasing predatory mites. The most important species are the oldest mites commercially available for biological control, Phytoseiulus persimilis (Hussey, N.W. and Scopes, NE A., 1985), Neoseilus californicus) (Wei-Lan Ma and Laing, JE, 1973). Both predatory mites are mass-bred with their natural host nymph mite in a bean plant in the greenhouse (Phaseolus vulgaris).

  In the scientific literature, several predatory mites that prey on whiteflies have been reported (Tech, Y. 1966; Swiski, E. et al., 1967; Nomikou, M. et al., 2001). Unfortunately, to date, there are still no predatory mites commercially available for biological control of whiteflies. Perhaps because predatory mites are known to prey on whiteflies, but their ability to use as an increasing biological control against whiteflies is not recognized in the art. During incremental biological control, biological agents are released into the crop for pest control. More importantly, the economics needed to allow the release of large numbers of predatory mites on crops, which is most important for their use performance as an increasing biological control factor. There is no mass breeding system available for the predatory mite species that may be effective against whiteflies available in the art.

  Instead, whiteflies are predatory parasitoids, such as Encarsia formosa and B. elegans, and tobacco plants (Eretmocerus cerevisiae), Greenhouse white lice (Trialeurodes vaporariorum) It is controlled by releasing a predatory parasitoid bee (Eretmocerus mundus) against whiteflies. In addition, several predators such as Macrophus caliginosus, which is a predatory insect, and Delphasus catalinae, which is a ladybird insect, are bred in large quantities and released. All these parasitoids and mass breeding of predators are accompanied by plant greenhouse production and whiteflies, which require considerable investment.

  Biological control of whiteflies and other crop pests with predatory mites that can be economically grown in large quantities using artificial host mites in the rearing medium uses a limited area for such rearing systems This is very advantageous. Furthermore, in such a system, predatory mites can be raised in a controlled artificial climate room. Therefore, it does not require large investments in greenhouses and crops.

  Recent studies have pointed out the potential of Amblyseius swiskii, a predatory mite as a highly efficient biological control factor for thrips (Apis thrips and Citrus thrips) and whiteflies (Onitsuna and Tobacco whiteflies) (Nomikou, M., Janssen, A., Schragag, R. and Sabelis, MW, 2001; Messlink, G. & Steenpaal, S. 2003; Messlink, G. 2004; Messellink, G. & Steenpaal, S 2004; Bolckmans, K. & Moerman, M. 2004; Messelink, G. & Pijnakker, J. 2004). Amblyseius swirskii showed a very strong number of responses to the presence of pests and plant pollen. This means that the number of ticks that must be released to achieve good biological control is much less compared to the black tickling mites. In one study, the release of one Amblyseius swirskii per leaf of a capsicum plant resulted in the same level of control of citrus white thrips as when 30 leaves of the black mite were released. (Bolckmans, K. & Moerman, M. 2004).

  In the art, pollen (Messelink, G. & Pijnakker, J. 2004) or lepidopterous insects, Corcyra cephalonica or eggs from Ephestia kuehnella (Romeith, A. H.). et al., 2004) are only disclosed for the breeding of Amblyseius swirskii.

  For pollen breeding, collect pollen of suitable plants such as large greenhouse area to produce plants such as castor bean plant (Toma sp.) Or outdoor pods (Typha spp.) is required. The collection of plant pollen outdoors requires a very large labor force and can only collect a limited amount. Plant pollen collected by bees is not suitable for breeding predatory mites.

  Breeding with lepidopteran insect eggs requires a large investment in egg production and is therefore very expensive.

  Due to the shortcomings of the dietary material available for Amblyseius swirskii, this predatory mite is not on the market in large quantities. Thus, an alternative dietary source that can economically mass produce Amblyseius Swiskii would make this predatory mite suitable for use as an increased biological control factor for use against various crop pests. Can and is beneficial.

  It has now been found that Amblyseius swirskii can be bred using an artificial host population comprising at least one acarid species.

  Thus, according to a first aspect, the present invention relates to a tick composition comprising a breeding population of the predatory burdock mite species Amblyseius swirskii and an artificial host population comprising at least one acarid species.

  Amblyseius swirskii Atias-Henriot, 1962, (Chant DA and McMurtry JA, 2004), (= Typhrodromips swirski et al., 200) (Athias-Henriot, 19). E. et al. , 1967 and Atias-Henriot, C.I. , 1962, from its natural host plant.

  Coniferous mites are similar to Hughes A. M.M. , 1977, and can be isolated from their natural habitat, and Parkinson, C., et al. L. (1992) and Solomon, M .; E. & Cunnington, A.C. M.M. (1963) can be maintained and cultured.

  Artificial host species are species that live in different natural habitats than predatory mites, but nonetheless, one or more life-period artificial hosts are suitable for at least one life-period predatory mite. A predator. Most importantly, predatory mite mites have the ability to develop, and have the ability to breed when raised using the diet of an artificial host and increase the number of individuals in that breeding population.

  The natural habitat of Amblyseius swirskii is on the plants that prey on pests (insects and ticks). Common mites are found as pests on preserved foods such as cereals and cereal products (eg, flour, bran), on dried fruits, or in other household settings.

  Thus, the composition of the present invention provides a new symbiosis of ticks, which does not occur naturally as the predatory mite Amblyseius swiskii lives in a different habitat.

  The composition of the present invention is not suitable only for mass breeding of Amblyseius swirskii. Since it includes the mobile predatory life of Amblyseius swirskii or the life that can develop into these mobile life, it can also be used as a biological crop protection factor.

A summary of the referenced families and species is provided in FIG.

  In a preferred embodiment, the composition includes a carrier for the individuals of the population. The carrier may be any solid material that is suitable for providing a carrier surface to an individual. Preferably, the carrier provides a porous medium capable of exchanging metabolic gases and heat generated by the tick population. Examples of suitable carriers are plant materials such as (wheat) bran, buckwheat husk, rice husk, sawdust, coarse corn and the like.

  More preferably, a dietary substance suitable for the artificial host population is added to the composition. Alternatively, the carrier itself can comprise a suitable dietary substance. Suitable dietary materials are Parkinson, C .; L. , 1992; Solomon, M .; E. & Cunnington, A.C. M.M. 1963; Chmielewski, W, 1971a; Chmielewski, W, 1971b or British Patent No. 2393890.

According to a preferred embodiment, the artificial host is
i) Carpolyphidae, for example
From the genus Carpolyphus, for example Carpolyphus lactis,
ii) Pyroglyphidae, for example
From the genus Dermatophagoides, such as Dermatophagoides pteronysinus or Dermatophagoides farinae,
From the genus Euroglyphus, such as Euroglyphus longior or Euroglyphus maynei,
From the genus Pyroglyphus, for example Pyroglyphus africanus,
iii) Glyciphagidae, for example
From the genus Glycyphagus, such as Glyciphagus descriptor or Glyciphagus domesticus,
From the genus Lepidoglyhus, such as Lepidoglyhus destructor,
iv) Acaridae, for example
From the genus Tyrophagus, such as Tyrophagus putrescentiae or Tyrophagus tropicus,
From the genus Acarus, for example, Acarus siro or Acarus faris,
It comprises at least one tick species selected from the genus Lardoglyhus, such as Lardoglyhus konoi.

  Artificial host tolerance may be observed between different Amblyseius swirskii strains. Furthermore, there are cases where it is possible to keep a line suitable for a specific artificial host by selective feeding.

  As used herein, the term “bred” should be understood to include propagation and increase of a population by sexual reproduction.

  Domesticated populations can include both sex adults and / or other life stage sex individuals capable of maturing into sexually mature adults, such as eggs and / or nymphs . Alternatively, the domestic population can include one or more fertilized females. In essence, this domestic population can increase its population by sexual reproduction.

  Preferably, said artificial host population is a captive population as defined above, eg it can maintain itself or even generate itself to some extent. When the artificial host is supplied as a breeding population, preferably a dietary material for the artificial host is also supplied. This dietary material is described in Solomon, M .; E. and Cunnington, A.M. M.M. Parkinson, C., 1963; L. , 1992; Ramakers, P .; M.M. J. et al. and van Lieburg, M.M. J. et al. 1982; British Patent No. 2,393,890 can be similar to the dietary material.

  Said artificial host is preferably selected from the family Carplyphydae, for example Carpolyphus, most preferably the dried fruit mite Carpolyphus lactis (Linne, 1758) (Acari: Scaridae) is there.

  Sugar mite is a universal species that occurs on and in various preserved organic materials. This is found primarily on dried fruits, such as dried figs, dried prunes, raisins, and on necrotic tissue pieces within the honeycomb (Hughes, AM, 1977; Chmilewski, W., 971). (A); Chmielewski, W., 1971 (b)). In contrast to mites, sugar mites do not cause crop damage. Thus, an artificial host from this preferred choice may be applied directly on or near the crop when the composition of the invention is used for crop protection in such a way that individuals of the artificial host population can come into contact with the crop. Or when used in sustained / controlled / sustained release sachets.

  A further advantage of sugar mite is that it is considered a universal species. Therefore, international trade in products containing it rarely encounters regulatory constraints that alien species encounter in many countries.

  Moreover, it turned out that a sugar mite is an artificial host especially suitable for Amblyseius swirskii. This is because the predator can be raised with this host for many life periods, and in all circumstances, all life periods.

  It should be understood that in a preferred embodiment the sugar mite is an artificial host, but in other embodiments, other than the mite family may be selected, for example, other than the mite genus, or in particular other than the mite.

  In this composition, the number of predatory mite species is about 1000: 1 to 1:20, for example about 100: 1 to 1:20, for example 1: 1 to 1: 10, preferably about 1: 4, 1: 5 or 1: 7.

  These relative numbers may depend on the particular intended use of the composition and / or the stage of development of the burdock mite population on the artificial host. In general, a composition in which an individual of an artificial host is present in excess relative to the number of the house dust mite is preferable for breeding the house dust mite species because sufficient prey is provided to the house dust mite. However, as the population of the tick mite population increases while preying on the artificial host, the relative number of individuals of the mite species increases.

  A composition that includes a high relative number of predatory mites may be formed from a composition that includes a lower relative number, and the prey of predatory mites can be increased by preying on artificial hosts. Alternatively, a composition comprising a low relative number of predatory mite can be combined with a composition comprising a higher relative number and a composition comprising only an artificial host (possibly in combination with a carrier and / or dietary material suitable for the artificial host). It can be formed by mixing with a composition containing a lower relative number.

  According to a further aspect, the present invention relates to a method for breeding the predatory burdock mite species Amblyseius swiskii. The method includes providing a composition of the present invention, and causing the predatory mite individual to prey on the individuals of the artificial host population.

  For optimal generation of the predatory mite, the composition is maintained at, for example, 18-35 ° C, preferably 20-30 ° C, more preferably 20-25 ° C, most preferably 22-25 ° C. . A suitable relative humidity range is between 75 and 95%, preferably between 80 and 90%. These temperatures and relative humidity ranges are generally also suitable for maintaining artificial host species.

  Preferably, the composition comprises a carrier capable of providing a porous medium and a dietary material suitable for the artificial host species, and the artificial host species is maintained as a three-dimensional culture on the carrier. In such a three-dimensional culture, members of the artificial host species can move freely in three dimensions. In this way, the artificial host species can spread over the larger volume of the carrier and more optimally utilize the dietary material. In view of the mobility stage size of Amblyseius swirskii relative to the individual of the artificial host, in general, Amblyseius swirskii will also invade the entire volume of its carrier when it is fed. Preferably, the three-dimensional culture is obtained by providing the support in a three-dimensional layer, ie a three-dimensional layer, whose two-dimensional dimensions are larger than one timed dimension. A specific example is a horizontal layer having a length and width on the metric order and a constant thickness on the centimeter order. A three-dimensional layer is preferred because it can sufficiently exchange metabolic heat and gas and provides a large production volume compared to a two-dimensional layer.

According to a further aspect, the present invention is directed to the use of acarid mite as an artificial host for breeding the predatory mite Amblyseius swirskii. The mite is preferably
i) Acaridaceae, for example
From the mite genus, for example sugar mite,
ii) house dust mites, such as
From the genus Leopard mite, such as Yake leopard mite, Kona leopard mite,
From the genus Euroglyphus, such as Euroglyphus longior or Euroglyphus maynei,
From the genus Pyroglyphus, for example Pyroglyphus africanus,
iii) Glycifagus, for example
From the genus Glyciphagus, such as Glyciphagus destructor or Glyciphagus domesticus,
From the genus Lepidoglyhus, such as Lepidoglyhus destructor,
iv) Acaridaceae, for example
From the genus Sphaeromycetes, for example, Scutellaria or Tyrophagus tropicus,
From the spider mite genus, for example, Ashibuto mite or Acarus faris,
It is selected from the genus Hosika mite, for example, Scarlet mite

  The acarid mites are preferably selected from the family Acaridaceae, for example from the genus Mite, and most preferably a mite for the reasons discussed above.

  According to a further aspect, the present invention relates to a rearing system for rearing the predatory burdock mite Amblyseius swirskii.

  The rearing system includes a container that holds the composition of the present invention. The container may be of any type suitable for restraining individuals from both populations. The rearing system can include means between the inside and outside to facilitate the exchange of metabolic gases and heat, such as vents. Such vents must prevent the individuals of the population from escaping from the container. This can be done, for example, by covering those vent holes with a net.

  This breeding system can be suitable for large-scale breeding of the burdock mite species. Alternatively, the rearing system can also be used to release predatory mites on crops. In this case, the container may be suitable for releasing a predatory mite in the mobile period at a certain time. This can be done by providing the container with a closed opening that can be opened. Alternatively, or in conjunction, the container may be provided with a relatively small release opening that limits the number of mobile phase mites leaving the container at a given time interval. Thus, this breeding system is based on Sampson, C.I. 1998, and British Patent No. 2,393,890, can function in a similar manner to a sustained or sustained release system.

  A rearing system for releasing predatory mite on a crop is preferably dimensioned so that it can be suspended in the crop or placed at the root of the crop. To hang in the crop, the container can be provided with suspending means such as cords or hooks.

  According to a further aspect, the present invention is directed to the use of the present composition or rearing system for controlling crop pests in commercial crops.

  The pests include whitefly, for example, white fly or tobacco whitefly; You can choose from. The predatory mite Amblyseius swirskii shows good efficacy in controlling these pests.

  Said crops are (greenhouse) vegetable crops, such as pepper (Capsicum annuum), eggplant (Solanum melogena), cucurbitaceae (Cucurbitaceae), such as cucumber (Cucumis sativa). Melons (Cucumis melo), watermelons (Watermelons); small fruits (eg, strawberries (Fragaria x ananassa), tree straws (Rubus ideas)); (greenhouse) crops (greenhouse) Rose, gerbera, chrysanthemum); or tree crops such as, but not limited to, citrus (Citrus spp.).

  Furthermore, the present invention relates to a method for controlling biological pests in a crop, comprising supplying the composition of the present invention to the crop.

  The pest can be selected as in the use of the present invention.

  In the method of the invention, the composition can be supplied by applying a certain amount of the composition in the vicinity of a number of crop plants, for example on or at the root. Since this composition is a general practice for utilizing predatory mite composition to control increased biological pests, the crop plant can be obtained by simply spraying it on the crop plant or at the root of the crop plant. Can be supplied to. The amount of the composition that can be supplied to each individual crop plant by spraying can range from 1 to 20 mL, such as 1 to 10 mL, preferably 2 to 5 mL.

  Alternatively, the composition can be supplied to a number of crop plants as a breeding system of the present invention suitable for releasing predatory mites on a crop. The rearing system can be placed in the vicinity of a large number of crop plants, for example in or at the root.

  In the biological pest control method of the present invention, it may not be necessary to supply this composition to all crop plants. This is because commodity crops are usually cultivated densely. Predatory dust mites can spread from one crop plant to another. The number of crop plants that must be supplied with the composition of the present invention to provide sufficient crop protection can depend on the specific environment, and can be readily determined by those skilled in the art based on experience. Usually, the number of predatory mites that are released per hectare is often determined. This number can range from 1000 to 3,000,000 per hectare, typically from 250,000 to 1,000,000 or 250,000 to 500,000 per hectare.

  In a further preferred embodiment of the biological pest control method of the invention, the crop is selected as described in connection with the use of the composition.

  The present invention will now be further described with reference to the following examples. These examples illustrate non-limiting embodiments of various aspects of the invention.

<Large breeding of Carablyphus lactis (sugar mite)>
A large amount of sugar mite was bred using a medium containing baker's yeast (Chmielewski, W., 1971 (a); Chmielewski, W., 1971 (b)).

  The culture is provided with a breathable container with a relative humidity of between 85 and 90% between 22 ° C and 25 ° C (eg sufficient vents with a 47 micrometer fine mesh to prevent ticks from escaping). In a bucket). By adding a new medium at least once a week, large-scale breeding can be carried out successfully. This amount will depend on the number of ticks in the medium, but generally add between 100 and 300% of the initial amount of the culture. The thickness of the rearing layer can be from 1 to 10 cm, but should not be too thick to ensure optimal exchange of metabolic gases, such as carbon dioxide and oxygen, and metabolic heat. The biomass weight percentage of the mite medium was between 20% and 30% when sugar mites were raised using this medium. In general, this population increases 2 to 4 times per week.

<Mass breeding of Amblyseius swirskii in sugar mite>
To ensure optimal exchange of metabolic gas and heat, with a breathable container with a 5-25 cm oat shell layer as a carrier (eg, with a 47 micron fine mesh to prevent ticks from escaping) Amblyseius swirskii are bred in a bucket with sufficient vents.

  This carrier layer should not be too thick to ensure an optimal exchange of metabolic gases, such as carbon dioxide and oxygen, and metabolic heat. At least once a week, sugar mite rearing populations are added to the vessel at a biomass weight percentage of 15% to 30% tick medium.

The amount of sugar mite to be added is calculated based on the number of predatory mites and sugar mites present in the breeding container. Optimally, after adding new sugar mites, the ratio of predators to prey should be between 1: 7 and 1:12. The culture is maintained in the breeding vessel at a temperature between 22 ° C. and 25 ° C., a relative humidity of 85 to 90% and a CO ₂ level of up to 750 ppm. In this way, the population of Amblyseius swirskii can be doubled to tripled per week. In general, a density of 100 to 500 predatory mites per gram of breeding support layer can be achieved.

<An egg-laying test of Amblyseius swirskii in young and mature life sugarcanes>
The purpose of this experiment was to determine if Amblyseius swirski prefers sugarcane mite juveniles (eggs, larvae and nymphs), or to cultivate Amblyseius swirski by using this artificial host during mature life Is to find out that it is possible. For this purpose, different breeding systems of Amblyseius swirskii (partially fed with juvenile sugar mites and others fed with adult sugar mites) were created. The difference in the average number of eggs born per Amblyseius swirskii female per day when the diet source is mature mite is compared to the case where the diet source is juvenile sugar mite.

Materials and Methods At the start of the experiment, Amblyseius swirskii adults were taken from Amblyseius swirskii mass cultures started several weeks ago. Thirty young adult females and 12 males were picked from this bulk culture and transferred to 6 newly prepared feeding containers. Five females and two males of Amblyseius swirskii were placed in each container. Three of them contained a sufficient amount of juvenile sugar mite as a food source. The remaining three test cultures were fed adult adult mites.

  Once six test cultures were prepared, they were placed in a climate chamber under controlled temperature (25 ° C.) and humidity (75%) conditions. They were removed after 2 or 3 days at this condition. Six new breeding containers similar to the previous one were prepared and the same 5 females and 2 males used previously were transferred. As in the previous stage, a sufficient amount of juvenile or mature sugar mite as a dietary source was added to each test vessel. After transferring males and females, the number of eggs in the breeding container after transferring them was counted.

  The old rearing system was stored in the climate chamber for 2 or 3 days for a second count to detect potentially hidden offspring and then destroyed. As with the old breeding system, the new one was saved and the same procedure was repeated. Every day, the amount of sugar mite in each breeding container was checked. A sufficient amount was added as needed.

  Data were obtained every 2 or 3 days both by evaluating the number of offspring in the new breeding system (first count) and by evaluating the number of offspring in the old breeding system (second count). Based on the number of females and based on the total amount of offspring found in each breeding container, the average number of eggs born per female per day was obtained.

Results <Mature sugar mite as a food source>
(Evaluation once every 2-3 days) When comparing the evolution of the number of eggs born for one female in all experiments, the average ranged from 1.27 to 2.07 eggs / female / day It is.

  The overall average for all periods is 1.80 eggs per female per day. The total amount of eggs born per female is about 29 over a 16 day period. Comparing the average number of eggs born per female per day for the first, second and third independent breeding containers, these are 1.84, 1.72 and 1.85, respectively. . The experimental data is presented in Table 1 below.

<Youth sugar mite as a food source>
(Evaluation once every 2-3 days) When comparing the evolution of the number of eggs born for one female in all experiments, the average ranged from 1.43 to 2.07 eggs / female / day It turned out to be.

  The average for all periods is 1.84 eggs per female per day. The total amount of eggs born for one female is about 33 over a period of 18 days. Comparing the average number of eggs born per female per day for three independent rearing containers, the averages for the first, second and third rearing systems are 1.72, 1.89 and 1.81. The results are shown in Table 2 below.

結果は、Ａｍｂｌｙｓｅｉｕｓ ｓｗｉｒｓｋｉｉが、幼若期と成熟期、両方のサトウダニを用いて繁殖できることを示している。

The results show that Amblyseius swirskii can be bred with both juvenile and mature sugar mites.

<Oviparation of Amblyseius swirskii in Tyrophagus putrescentiae>
In the same general experimental outline as described in Example 3, the average number of eggs born for one Amblyseius swirskii female was determined when the mite was used as a dietary source.

  However, in this experiment, no distinction was made between juveniles and adults of the artificial host. Instead, individuals from the Kenagakonadani population were added non-selectively.

Results Comparing the evolution of the number of eggs born for one female in all experiments (assessed once every 2-3 days), the average is 0.84-1.60 eggs / female / day It is a range. The final average for the entire period is 1.23 eggs per female per day. The total amount of eggs born for one female is about 20 over a period of 17 days. Comparing the average number of eggs born per female per day for three independent rearing containers, the averages for the first, second and third rearing containers were 1.17, 1.28 and 1.23. This data is presented in Table 3 below.

結果は、Ａｍｂｌｙｓｅｉｕｓ ｓｗｉｒｓｋｉｉが、ケナガコナダニを用いて繁殖できることを示している。

The results show that Amblyseius swirskii can be propagated using the mite.

[References]

Claims (23)

A breeding population of the predatory burdock mite species Amblyseius Swiskii;
An artificial host population comprising at least one Astigmatid mite species;
Optionally, a tick composition comprising a carrier for individuals of said population. The mite is
i) Carpolyphidae, for example
From the genus Carpolyphus, for example Carpolyphus lactis,
ii) Pyroglyphidae, for example
From the genus Dermatophagoides, such as Dermatophagoides pteronysinus or Dermatophagoides farinae,
From the genus Euroglyphus, such as Euroglyphus longior or Euroglyphus maynei,
From the genus Pyroglyphus, for example Pyroglyphus africanus,
iii) Glyciphagidae, for example
From the genus Glycyphagus, such as Glyciphagus descriptor or Glyciphagus domesticus,
From the genus Lepidoglyhus, such as Lepidoglyhus destructor,
iv) Acaridae, for example
From the genus Tyrophagus, such as Tyrophagus putrescentiae or Tyrophagus tropicus,
From the genus Acarus, for example, Acarus siro, Acarus faris,
2. Composition according to claim 1, selected from the genus Lardoglyhus, such as Lardoglyhus konoi, preferably selected from (i).   3. A composition according to claim 1 or 2, comprising a dietary substance suitable for the artificial host population.   The composition according to any one of claims 1 to 3, wherein the artificial host population is a breeding population.   The predatory mite species population is about 100: 1 to 1:20, for example about 1: 1 to 1:10, for example about 1: 4, 1: 5 or 1 relative to the number of artificial host individuals. : The structure of any one of Claims 1-4 which is 7. A method for rearing a predatory burdock mite species Amblyseius Swiskii comprising:
Providing the composition according to any one of claims 1 to 5,
Feeding the individuals of the predatory mite mites to individuals of the artificial host population.   The method of claim 6, wherein the composition is maintained at 18-35 ° C. and / or 60-95% relative humidity.   The method according to claim 6 or 7, wherein the composition comprises a carrier and a suitable dietary substance, and the artificial host population is maintained as a three-dimensional breed on the carrier.   Use of acarid mite as an artificial host for rearing predatory mite Amblyseius Swirlii. The mite is
i) Acaridaceae, for example
From the mite genus, for example sugar mite,
ii) house dust mites, such as
From the genus Leopard mite, such as Yake leopard mite or Kona leopard mite,
From the genus Euroglyphus, such as Euroglyphus longior or Euroglyphus maynei,
From the genus Pyroglyphus, for example Pyroglyphus africanus,
iii) Glycifagus, for example
From the genus Glyciphagus, such as Glyciphagus destructor or Glyciphagus domesticus,
From the genus Lepidoglyhus, such as Lepidoglyhus destructor,
iv) Acaridaceae, for example
From the genus Sphaeromycetes, for example, Scutellaria or Tyrophagus tropicus,
From the spider mite genus, for example, Ashibuto mite or Acarus faris,
10. Use according to claim 9, selected from the genus Acarina, such as acarid mite, preferably selected from (i).   A breeding system for breeding a predatory mite Amblyseius swirskii, comprising a container for holding the composition according to any one of claims 1 to 5.   12. The breeding system of claim 11, wherein the container includes an outlet for at least one mobile life of the mite.   13. A breeding system according to claim 12, wherein the outlet is suitable for providing sustained release of the at least one mobile life.   Use of the composition according to any one of claims 1 to 5 for crop pest control in commercial crops.   15. Use according to claim 14, wherein the composition is supplied by a breeding system according to any one of claims 11-13.   The crop pest is a whitefly, such as Trialeurodes vaporariorum or Beisia tabaci; a thrips such as Thrips tabaci ), Mites, such as Tetanychus urticae, dust mites, such as Polyphagotarsonmus latus, 16. Use according to claim 14 or 15.   The crop is a (greenhouse) vegetable crop, such as pepper (Capsicum annuum), eggplant (Solanum melogena), cucurbitaceae (Cucurbitaceae), such as cucumber (Cucumis sativa) Melons (Cucumis melo), watermelons (Watermelons); small fruits (eg, strawberries (Fragaria x ananassa), tree straws (Rubus ideas)); (greenhouse) crops (greenhouse) 17. Use according to any one of claims 14 to 16, selected from e.g. roses, gerberas, chrysanthemum); or tree crops such as citrus (Citrus spp.).   A method for controlling biological pests in a crop, comprising supplying the crop with the composition of any one of claims 1-5.   19. The pest is selected from whitefly, such as whitefly or tobacco whitefly; thrips, such as white thrips, or sand thrips, such as citrus white thrips; spider mites, such as nymph mites; The method described.   20. A method according to claim 18 or 19, wherein the composition is supplied by applying a certain amount of the composition to a number of crop plants, preferably in the vicinity of each crop plant, e.g. at the root.   21. A method according to claim 20, wherein the amount is 1-10 mL, preferably 2-5 mL.   14. The rearing system according to claim 11, wherein the rearrangement system is arranged in the vicinity of a number of food plants, preferably in the vicinity of each food plant. The method according to any one of claims 18 to 21, wherein the method is supplied by hanging. The crop is a (greenhouse) vegetable crop, such as pepper (Capsicum annuum), eggplant (Solanum melogena), cucurbitaceae (Cucurbitaceae), such as cucumber (Cucumis sativa) , Melon (Cucumis melo), watermelon (Citrullus lananthus); small fruit (eg, strawberry (Fragaria x ananassa)), koji (Rubus ideaus)); (greenhouse) viewing crop 23. A method according to any one of claims 18 to 22 selected from (e.g. rose, gerbera, chrysanthemum); or tree crops, e.g. Citrus spp.

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