JP2011105622A - Hyaluronidase activity inhibitor and external preparation for skin - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a more highly active hyaluronidase activity inhibitor than conventional inhibitors, and to provide an external preparation for skin, having effects such as not only a dry skin-improving effect (moisture-retaining effect) and a chapped skin-improving effect, but also a skin tension-improving effect and a skin gloss-improving effect, which have not been obtained by conventional hyaluronidase activity inhibitors. <P>SOLUTION: The hyaluronidase activity inhibitor includes a Carex humilis extract as an active ingredient. The external preparation for skin contains a Carex humilis extract in an amount of 0.0001 to 10 mass% as a dry residue. <P>COPYRIGHT: (C)2011,JPO&INPIT

Description

  The present invention relates to a novel hyaluronidase activity inhibitor and a skin external preparation containing a specific plant extract. More specifically, the present invention relates to a highly active hyaluronidase activity inhibitor containing a Carex humilis extract as an active ingredient, and a topical skin preparation containing a specific amount of the fungus lizard extract. The skin external preparation of the present invention is excellent in the rough skin improving effect, the dry skin improving effect (moisturizing effect), the skin firmness improving effect, and the skin gloss improving effect.

Hyaluronic acid is a type of glycosaminoglycan present in the extracellular matrix in the skin dermis and is involved in the retention of moisture in the skin. However, the amount of hyaluronic acid in the tissue decreases with age, and along with this, the freshness of the skin is lost.
On the other hand, hyaluronidase is an enzyme that degrades hyaluronic acid, and hydrolyzes β (1 → 4) bonds in hyaluronic acid. Therefore, it is considered that by inhibiting the activity of hyaluronidase, the amount of hyaluronic acid in the skin dermis can be maintained, and fresh and firm skin can be maintained (Non-patent Document 1).

  Among hyaluronic acids, high molecular hyaluronic acid shows a moisturizing effect, but low molecular weight hyaluronic acid of 20 kDa or less promotes the production of inflammatory cytokines (Non-patent Document 2). It is thought to be involved. Therefore, hyaluronidase activity inhibitors that inhibit the activity of hyaluronidase, which decomposes high molecular hyaluronic acid to produce low molecular hyaluronic acid, not only moisturize by maintaining the amount of hyaluronic acid, but also the production of low molecular hyaluronic acid. Anti-inflammatory effect, anti-allergic effect, skin roughening improving effect, etc. can be expected by suppressing.

  Furthermore, hyaluronic acid is also present in large amounts in tissues other than the skin, such as joint cavities, articular cartilage, and the vitreous body of the eye, and the amount of hyaluronic acid in the joint decreases, leading to joint pain. The agent can be used as a preventive agent for joint pain and the like. Moreover, in the process of cancer cell metastasis, hyaluronidase is an enzyme that functions when migrating between tissues (Non-patent Document 3), so it can be used as a drug that inhibits cancer progression and metastasis. It is done. Furthermore, the hyaluronidase activity inhibitor can be expected to increase the stability of the hyaluronic acid-containing preparation.

  To date, as hyaluronidase activity inhibitors, hot water extracts of algae (Patent Document 1), extracts such as Rahan fruit (Patent Document 2), tamarind husk extract (Patent Document 3), neem, Koiusigaya, Murayako Enigii, Sfaransus Various plant extracts such as extracts such as Indix, Ginkgo biloba, Ibonashi Tsurugafuji (Patent Document 4), extracts such as Camellia (Patent Document 5), and chemistry such as sodium cromoglycate (Non-Patent Document 4) Synthetic compounds have been reported.

  However, since conventional hyaluronidase activity inhibitors are not sufficiently effective, there have been cases where a large amount of the hyaluronidase activity inhibitor has to be added to the external preparation for skin. When a large amount of a plant extract as a hyaluronidase activity inhibitor is blended in a large amount, the stability of the preparation may be deteriorated, and many are accompanied by changes in odor, color and the like. Moreover, since many hyaluronidase activity inhibitors obtained by chemical synthesis are used as pharmaceuticals, it is necessary to pay attention to the dosage in use. Furthermore, the conventional hyaluronidase activity inhibitors had a skin moisturizing effect and an anti-inflammatory effect, but they did not have an effect of improving skin firmness due to insufficient inhibitory activity. Moreover, none of the skin external preparations has an effect of improving skin gloss.

JP-A-10-265399 JP-A-6-80576 JP-A-8-231347 JP 7-138180 A JP 2003-012489 A French Patent Application No. 2000-15098 (French Patent Invention No. 2816843) French Patent Application No. 2002-11628 (French Patent Invention No. 2844714) French Patent Application No. 2006-2294 (French Patent Invention No. 2898493) Korean Patent Application No. 2007-52002 (Korea Patent Publication No. 2008-104759)

Life Sciences, 2007, Vol.80, No.21, p.1921-1943 Matrix Biology, 2002, Vol.21, p.25-29 Cancer Research, 1996, Vol.56, No.3, p.651-657 Chemical & Pharmaceutical Bulletin, 1985, Vol.33, No.2, p.642-646 American Journal of Chinese Medicine, 2004, Vol.32, No.4, p.521-530

  An object of the present invention is to provide a hyaluronidase activity inhibitor having a higher activity than conventional ones, and to provide not only a moisturizing effect and a rough skin improving effect, but also a conventional hyaluronidase such as a skin elasticity improving effect and a skin gloss improving effect. An object of the present invention is to provide a skin external preparation having an effect that cannot be obtained with an activity inhibitor.

  In order to solve the above-mentioned problems, the present inventors have made extensive studies using various plant extracts on the hyaluronidase activity inhibitory action involved in skin moisturization, anti-inflammatory action and the like. As a result, it was found that the extract of Hosobakageage has an excellent hyaluronidase activity inhibitory action. In addition, it has been found that by adding a specific amount of the extract of Hosoba lizard to a skin external preparation, not only a moisturizing effect and a rough skin improving effect, but also a skin firmness improving effect and a skin gloss improving effect can be obtained. The invention has been completed.

  That is, the present invention is a hyaluronidase activity inhibitor containing a Carex humilis extract as an active ingredient, and contains 0.0001 to 10% by mass of the Carex humilis extract as a dry residue. It is a skin external preparation.

Carex humilis is a perennial plant belonging to the genus Spodoptera, and is a plant distributed throughout Northeast Asia. As for the extract of the mosquito lizard, 5α-reductase inhibitory action (patent document 6), skin care ingredient for skin with broken hormone balance (patent document 7), cosmetic composition for delayed aging (patent document 8), tyrosinase inhibitory action There are reports on whitening effect (Patent Document 9) and anti-inflammatory action (Non-Patent Document 5).
However, Hosoba lizard extract has an excellent hyaluronidase activity inhibitory action, and by including a specific amount of Hosoba lizard extract in a skin external preparation, not only moisturizing effect and skin roughening effect, but also skin elasticity improving effect It has not yet been reported that a skin gloss improvement effect can be obtained.

  The hyaluronidase activity inhibitor of the present invention has an excellent inhibitory effect at a lower concentration than conventional hyaluronidase activity inhibitors. In addition, the external preparation for skin of the present invention has not only a moisturizing effect and a rough skin improving effect, but also has an excellent skin elasticity improving effect and skin gloss improving effect, and further has good conformability to the skin and is used. There is no stickiness afterwards, and it has the effect of excellent stability over time.

Hereinafter, the present invention will be described in detail.
Each of the hyaluronidase activity inhibitor and the external preparation for skin of the present invention contains a Hosoba lizard extract. First, the Hosoba lizard extract will be described.

[Hosoba lizard extract]
The extract of the squirrel lizard used in the present invention is the root, leaf, flower, or seed (particularly preferably the root) of the sedge genus Carex humilis belonging to the genus Spodoptera (Carex humilis). It is extracted and refers to the extract itself, or its dilution or concentrate.

  Examples of the solvent used for extraction include hydrocarbons, esters, ketones, ethers, halogenated hydrocarbons, water-soluble alcohols and water. Among them, preferred are those using one or more of water, lower alcohol and polyhydric alcohol, and more preferred are those using one or more of water, ethanol and 1,3-butylene glycol. is there.

  The extraction method is in accordance with a conventional method, and the target extract can be obtained by immersing and filtering one or two or more solvents for 1 hour or more after drying the dried sow beetle as it is or after drying. Extraction can be carried out at normal pressure or under pressure, under reduced pressure, at room temperature or under heating and cooling. Furthermore, a supercritical extraction method using carbon dioxide or the like, a subcritical extraction method, a method of extraction using distillation such as steam distillation, a method of pressing and extracting scallops, a reflux extraction method, or the like can also be used. The obtained extract may be concentrated by distilling off the solvent, or purified by treatment such as column chromatography or solvent fractionation.

[Hyaluronidase activity inhibitor]
The hyaluronidase activity inhibitor of the present invention contains the above-mentioned Hosoba lizard extract as an active ingredient, and is a pharmaceutical, quasi-drug, food, cosmetics (for example, lotion, milky lotion, cream, gel, cosmetic liquid, Packs, oils, ointments, sprays, patches, etc.). Examples of the use as a pharmaceutical include use as an adjuvant for suppressing the degradation of hyaluronic acid used in joint function improving agents, ophthalmic surgery adjuvants, submucosal injections for endoscopes, eye drops and the like. It is done. It can also be used as an injection for the treatment of osteoarthritis and rheumatoid arthritis and for pain relief. The hyaluronidase activity inhibitor of the present invention contains additives that can be added to pharmaceuticals, quasi-drugs, foods, and cosmetics as long as they do not impair the effects of the present invention, as required, in addition to the extract of Hosoba lizard extract. May be.

[Skin external preparation]
The external preparation for skin of the present invention contains 0.0001 to 10% by mass of the above-mentioned Hosoba lizard extract as a dry residue. The mass of the dry residue in the present invention is not only the mass of the residue actually dried after removing the solvent, but also the mass of the residue obtained by calculating the amount of solvent contained in the residue and subtracting the amount of the solvent. Conceptually included. For example, when the extraction solvent is volatile, the extraction solvent is completely distilled off at 105 ° C. to 120 ° C., and is the mass of the remaining solid content. When the extraction solvent is nonvolatile, high performance liquid chromatography The amount of the solvent is quantified by, for example, and the amount of the other components is the mass of the dry residue.

  The blending amount of Hosoba lizard extract is 0.0001 to 10% by mass as a dry residue, preferably 0.0005 to 7.5% by mass, and more preferably 0.001 to 5% by mass. If the blending amount is less than 0.0001% by mass, the effect of improving rough skin, moisturizing effect, and improving skin firmness and gloss cannot be exhibited, and if it exceeds 10% by mass, the stability of the preparation tends to occur, which is not preferable. .

  In order to further improve the performance of the external preparation for skin of the present invention, an α-hydroxy acid (hereinafter also referred to as AHA) and / or a salt thereof, which is an organic acid having a hydroxyl group at the α-position of the carboxylic acid, is used. It is preferable to contain. Examples of AHA include citric acid, lactic acid, malic acid, tartaric acid, glycolic acid, etc. Examples of AHA salts include alkali metal salts, alkaline earth metal salts, ammonium salts, amine salts, organic amine salts, basic amino acids. A salt etc. are mentioned, The 1 type (s) or 2 or more types chosen from these AHA and its salt are used. Among these, it is preferable to contain lactic acid or a salt thereof. The compounding quantity of AHA and / or its salt is 0.001-5 mass%, Preferably it is 0.01-1 mass%. If it contains in 0.001-5 mass%, the texture improvement effect of skin and the gloss improvement effect of skin can be improved notably.

  The external preparation for skin of the present invention can be prepared in various dosage forms. For example, it can be used as a lotion, milky lotion, cream, gel, cosmetic liquid, pack, oil, ointment, spray, patch, etc. it can. In the present invention, additives commonly used in external preparations for skin such as cosmetics and pharmaceuticals can be appropriately blended as long as the effects of the present invention are not impaired.

  EXAMPLES Hereinafter, although an Example demonstrates this invention further in detail, this invention is not restrict | limited to the following Example. Unless otherwise indicated,% represents mass%.

(A) Preparation of Hosoba-hikage extract (hyaluronidase activity inhibitor) 500 g of 70% ethanol aqueous solution was added to 50 g of the collected Hosoba-kagege root, washed with pure water and dried, and extracted for 24 hours. After filtration, the obtained extract was deodorized and decolored, and further concentrated to obtain a powder of Lithophorus edulis extract (hyaluronidase activity inhibitor).

(B) Preparation of Camellia Flower Extract To 50 g of dried camellia flower, 500 g of 50% 1,3-butylene glycol aqueous solution was added, extracted at room temperature for 7 days, filtered, and cooled at −5 ° C. for 7 days. And the camellia flower extract was obtained by filtering again.

[Example 1, Comparative Examples 1 and 2: Hyaluronidase activity inhibition test]
The above-mentioned Hosoba-kagege extract powder was dissolved in an 80% aqueous ethanol solution so as to be 10 mg / mL, and used for the test. Further, the above-mentioned extract of Lilium medaka, sodium cromoglycate (MP Biomedicals, Inc.), and the above-mentioned camellia flower extract were appropriately diluted with a 30% 1,3-butylene glycol aqueous solution to obtain test solutions having concentrations shown in Table 1. .

To the 200 μL test solution, 100 μL of 400 U / mL sheep testicular hyaluronidase solution prepared with 0.1 M acetate buffer (pH 4.0) was added and incubated at 37 ° C. for 20 minutes. Thereafter, 0.1 mg / mL compound 48/80 of an enzyme activation reagent prepared with 0.1 M acetate buffer (pH 4.0) was added, and the mixture was incubated at 37 ° C. for 20 minutes. To this, 500 μL of 0.4 mg / mL rooster and fish-derived hyaluronic acid / 0.1 M acetic acid buffer (pH 4.0) was added and left at 37 ° C. for 40 minutes to carry out the enzyme reaction. After the reaction, 200 μL of 0.4N NaOH aqueous solution was added and ice-cooled to stop the enzyme reaction. Further, 200 μL of 0.8 M sodium borate aqueous solution (pH 9.1) was added and heated at 100 ° C. for 5 minutes. Ice-cooled after heating, 6 mL of color reagent (10 g of p-dimethylaminobenzaldehyde (manufactured by Wako Pure Chemical Industries, Ltd.) dissolved in 12.5 mL of 10N hydrochloric acid and 87.5 mL of acetic acid, diluted 10-fold with acetic acid) And allowed to stand at 37 ° C. for 20 minutes for color development. The amount of N-acetylglucosamine produced by the enzymatic reaction was measured as the absorbance at 585 nm (A585).
The inhibition rate of hyaluronidase activity was calculated using the following formula. The results are shown in Table 1.
Inhibition rate (%) = (A585Control−A585Sample) / (A585Control−A585Blank) × 100

  From the results shown in Table 1, it was clarified that the mosquito lizard extract exhibits a hyaluronidase activity inhibitory action superior to conventional plant extracts and synthetic compounds, and also exhibits an inhibitory activity even at a lower concentration than the conventional one. .

[Examples 2, 3, 4, Comparative Examples 3, 4, 5, 6: Sensory evaluation]
Using 80% ethanol aqueous solution of Hosoba lizard extract powder subjected to the hyaluronidase activity inhibition test, 30% 1,3-butylene glycol aqueous solution, sodium cromoglycate of camellia flower extract subjected to the hyaluronidase activity inhibition test, A lotion (external preparation for skin) shown in Table 2 was prepared, and 7 items were evaluated according to the following evaluation criteria. The results are shown in Table 2.

(Evaluation items and evaluation criteria)
(1) Skin roughening effect 20 females (25 to 52 years old) with rough skin are used as panelists, and the skin condition after using a topical skin preparation twice a day for 2 weeks is as follows. Evaluation was performed.
2 points: When it is felt that rough skin has been clearly improved.
1 point: When it feels that the rough skin has improved a little.
0 point: When the rough skin improvement effect is not seen at all, or when rough skin feels worse.

Furthermore, the evaluation of 20 persons was added and the total score was determined according to the following criteria.
A: 35 or more (external preparation for skin having a very excellent effect on improving rough skin)
○: 30 points or more and less than 35 points (external preparation for skin having an excellent effect on improving rough skin)
Δ: 15 points or more and less than 30 points (skin external preparation having a slight skin roughening effect)
×: Less than 15 points (external preparation for skin having no effect on rough skin)

(2) Improvement effect on dry skin (moisturizing effect)
20 females (25 to 56 years old) who feel dryness of the skin are used as panelists, and after using the topical skin preparation twice a day for 4 weeks, the condition of the skin 30 minutes after face washing is as follows. The sensory evaluation was performed.
2 points: When the skin feeling after cleansing is clearly improved.
1 point: When the feeling of tightness of the skin after face washing is slightly improved.
0 point: When the skin feeling after washing the face does not change, or when the feeling of tension is worse than before.

Furthermore, the evaluation of 20 persons was added and the total score was determined according to the following criteria.
◎: 35 points or more (external preparation for skin having excellent dry skin improvement effect)
○: 30 points or more and less than 35 points (external preparation for skin having excellent dry skin improvement effect)
Δ: 15 points or more and less than 30 points (skin external preparation having a slight dry skin improvement effect)
X: Less than 15 points (external preparation for skin having no improvement effect on dry skin)

(3) Skin elasticity improvement effect 20 females (25 to 56 years old) were panelists, and the skin condition after using a topical skin preparation twice a day for 4 weeks was subjected to sensory evaluation as follows. .
2 points: When the skin feels to have improved clearly.
1 point: When the skin feels slightly improved.
0 point: When the skin did not change, or when it felt that the skin became poor.

Furthermore, the evaluation of 20 persons was added and the total score was determined according to the following criteria.
A: 35 points or more (external preparation for skin having excellent skin elasticity)
○: 30 points or more and less than 35 points (external preparation for skin having excellent skin elasticity improvement effect)
Δ: 15 points or more and less than 30 points (skin external preparation having a slight skin elasticity improving effect)
×: Less than 15 points (external preparation for skin having no effect on skin elasticity)

(4) Skin shine improvement effect 20 women (25 to 56 years old) were panelists, and the skin condition after using the skin external preparation twice a day for 2 weeks was subjected to sensory evaluation as follows. .
2 points: When the skin gloss is clearly improved.
1 point: When the skin feels somewhat improved.
0 point: When the skin did not change, or when it felt that the gloss of the skin deteriorated.

Furthermore, the evaluation of 20 persons was added and the total score was determined according to the following criteria.
A: 35 or more (external preparation for skin having a very excellent skin gloss improving effect)
○: 30 points or more and less than 35 points (external preparation for skin having excellent skin gloss improvement effect)
Δ: 15 points or more and less than 30 points (skin external preparation having a slight skin gloss improving effect)
×: Less than 15 points (external preparation for skin having no skin gloss improving effect)

(5) Familiarity to the skin Twenty women (25 to 56 years old) were panelists, and the sensory evaluation was performed as follows for the feel when the skin external preparation was used after washing the face.
2 points: When you feel familiar with the skin during use.
1 point: When the user feels that the familiarity with the skin is slightly bad.
0 points: When the user feels that the familiarity with the skin is clearly bad.

Furthermore, the evaluation of 20 persons was added and the total score was determined according to the following criteria.
A: 35 points or more (external preparation for skin having excellent skin adaptability)
○: 30 points or more and less than 35 points (external preparation for skin having excellent adaptability to skin)
Δ: 15 points or more and less than 30 points (skin external preparation having slight skin conformability)
X: Less than 15 points (external preparation for skin having no conformability to skin)

(6) Stickiness after use Twenty women (25 years old to 56 years old) were panelists, and after washing their face, the skin feel 10 minutes later was subjected to a sensory evaluation using a skin external preparation.
2 points: When there is no stickiness.
1 point: When the skin feels somewhat sticky.
0 points: When the skin feels very sticky.

Furthermore, the evaluation of 20 persons was added and the total score was determined according to the following criteria.
◎: 35 points or more (external skin preparation without stickiness after use)
○: 30 points or more and less than 35 points (skin external preparation with little stickiness after use)
Δ: 15 points or more and less than 30 points (external preparation for skin having a little stickiness after use)
×: Less than 15 points (skin external preparation with stickiness after use)

(7) Stability over time The cosmetic was sealed in a transparent glass container and stored at 0 ° C., 25 ° C., and 40 ° C. for 3 months. The appearance was observed and evaluated in two stages as shown below.
○: Good stability (no change in appearance at any temperature)
X: poor stability (precipitation occurs or separates at any temperature, or discoloration occurs)

  From the results of Examples 2, 3, and 4, the lotion using the component of the extract of the squirrel lizard according to the present invention has not only a rough skin improvement effect and a moisturizing effect, but also an excellent skin elasticity and gloss improvement effect. In addition, it can be understood that the skin is well-fitted to the skin, has a good feeling of use such as stickiness, and has a good stability.

  On the other hand, in Comparative Example 3 containing no hyaluronidase activity inhibitor, sufficient effects were not obtained in any of the rough skin improvement effect, the moisturizing sustaining effect, and the skin firmness and gloss improvement effect. Moreover, although the lotion of the comparative examples 4 and 5 contains the camellia flower extract known as a hyaluronidase activity inhibitor, in any of a rough skin improvement effect, a moisturizing effect, and a skin firmness and gloss improvement effect, it is a comparative example. Similar to 3, no effect was observed. Further, in Comparative Example 6 containing sodium cromoglycate, as in Comparative Example 3, the effect of improving skin roughness, the effect of moisturizing, and the effect of improving skin firmness and gloss were not obtained.

[Examples 5 and 6, Comparative Examples 7 and 8: Sensory evaluation]
The emulsions shown in Table 3 were prepared using the powder of Hosoba lizard extract obtained in (A) above and sodium cromoglycate, and evaluated in the same manner as in Examples 2-4. In addition, the preparation of the emulsion containing Hosoba lizard extract powder was prepared by dissolving the Hosoba lizard extract powder obtained in (A) above in the polyhydric alcohol and nonionic surfactant component shown in Table 3 in advance. It was performed by blending with the ingredients of The results are shown in Table 3.

  From the results of Examples 5 and 6, the milky lotion using the components of the extract of the squirrel lizard extract according to the present invention is excellent in rough skin improvement effect, moisturizing effect, skin firmness and gloss improvement effect, and conformability to the skin, The feeling of use such as stickiness was also excellent, and the stability was also good. In particular, in the preparation of Example 6 in which Hosoba-hikage extract and AHA salt (sodium lactate) were used in combination, not only the rough skin improvement effect and the moisturizing effect but also the firmness and gloss improvement effect of the skin were remarkably increased.

  On the other hand, in Comparative Example 7 which does not contain a hyaluronidase activity inhibitor, sufficient effects were not obtained in any of the rough skin improvement effect, the moisturizing sustaining effect, and the skin firmness and gloss improvement effect. Further, in Comparative Example 8 in which sodium cromoglycate and AHA salt (sodium lactate) were used in combination, as in Comparative Example 7, the rough skin improvement effect, the moisturizing effect, and the skin firmness and gloss improvement effect were not obtained.

[Examples 7 and 8, Comparative Example 9: Sensory evaluation]
Creams shown in Table 4 were prepared by using the powder of extract of Lilium fountain obtained in (A) above, and evaluated by the same method as in Examples 2-4. In addition, preparation of the cream containing Hosoba lizard extract powder is the same as in the case of the emulsion. The polysodium alcohol and nonionic surfactant components shown in Table 4 are obtained from the Hosoba lizard extract powder obtained in (A) above. This was carried out by dissolving in advance and then blending with other components. The results are shown in Table 4.

  From the results of Examples 7 and 8, the cream using the component of the extract of the squirrel of the present invention according to the present invention has a rough skin improving effect, a moisturizing effect, and a firmness of the skin as compared with Comparative Example 9 not containing a hyaluronidase activity inhibitor.・ Excellent gloss improvement effect, good fit to skin, feeling of use such as stickiness, and stability. In particular, in the preparation of Example 8 in which Hosoba lizard extract and AHA salt (sodium lactate) were used in combination, not only the rough skin improvement effect and the moisturizing effect but also the skin firmness and gloss improvement effect were remarkably increased.

[Summary]
As described above in detail, it has been clarified that the hyaluronidase activity inhibitor of the present invention exhibits higher activity at a lower concentration than conventional ones. Therefore, the external preparation for skin containing the extract of Hosoba lizard, which is an active ingredient of the hyaluronidase activity inhibitor of the present invention, is a rough skin improving effect and an improved dry skin effect that are recognized in conventional external preparations containing hyaluronidase activity inhibitors. Not only was it superior to (moisturizing effect), but it also had superior skin firmness and gloss improvement effects that could not be obtained with such conventional external preparations for skin. In addition, it was excellent in skin feel, stickiness, and the like, and had good stability. Furthermore, the external preparation for skin using the Hosoba lizard extract according to the present invention in combination with AHA or a salt thereof remarkably enhances the skin elasticity improvement effect and the skin gloss improvement effect.

  The hyaluronidase activity inhibitor of the present invention is used as pharmaceuticals, quasi drugs, foods, cosmetics (for example, lotions, emulsions, creams, gels, serums, packs, oils, ointments, sprays, patches, etc.). be able to. Moreover, the skin external preparation of this invention can be utilized as a pharmaceutical, a quasi-drug, and cosmetics.

Claims (2)

  A hyaluronidase activity inhibitor containing a Carex humilis extract as an active ingredient.   An external preparation for skin containing 0.0001 to 10% by mass of a carex humilis extract as a dry residue.