JP2011041539A - Method for producing long chain polyunsaturated fatty acid of liverwort - Google Patents

Method for producing long chain polyunsaturated fatty acid of liverwort Download PDF

Info

Publication number
JP2011041539A
JP2011041539A JP2009192962A JP2009192962A JP2011041539A JP 2011041539 A JP2011041539 A JP 2011041539A JP 2009192962 A JP2009192962 A JP 2009192962A JP 2009192962 A JP2009192962 A JP 2009192962A JP 2011041539 A JP2011041539 A JP 2011041539A
Authority
JP
Japan
Prior art keywords
acid
liverwort
polyunsaturated fatty
chain polyunsaturated
content
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2009192962A
Other languages
Japanese (ja)
Other versions
JP5374710B2 (en
Inventor
Yukio Okimura
幸夫 沖村
達朗 ▲濱▼田
Tatsuro Hamada
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ishikawa Prefecture
Hokuriku Electric Power Co
Original Assignee
Ishikawa Prefecture
Hokuriku Electric Power Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ishikawa Prefecture, Hokuriku Electric Power Co filed Critical Ishikawa Prefecture
Priority to JP2009192962A priority Critical patent/JP5374710B2/en
Publication of JP2011041539A publication Critical patent/JP2011041539A/en
Application granted granted Critical
Publication of JP5374710B2 publication Critical patent/JP5374710B2/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Landscapes

  • Cultivation Of Plants (AREA)

Abstract

<P>PROBLEM TO BE SOLVED: To provide a method for increasing the content of arachidonic acid and eicosapentaenoic acid in liverwort. <P>SOLUTION: The liverwort includes long chain polyunsaturated fatty acids such as arachidonic acid and eicosapentaenoic acid which higher plants do not have. The content of the fatty acids in the liverwort is small, but when cultivating by irradiating blue light to the liverwort, hexadecatrienoic acid (16:3) and α-linolenic acid (18:3n-3) are decreased, and palmitic acid (16:0), arachidonic acid (20:4n-6) and eicosapentaenoic acid (20:5n-3) are increased by blue light, compared to LED light of other wavelength. <P>COPYRIGHT: (C)2011,JPO&INPIT

Description

本発明は、ゼニゴケの長鎖多不飽和脂肪酸の含有量を増やす方法に関する。   The present invention relates to a method for increasing the content of long-chain polyunsaturated fatty acids of Xenopus.

ゼニゴケは、高等植物が有しないアラキドン酸やエイコサペンタエン酸といった長鎖多不飽和脂肪酸を含有する。これらの脂肪酸は健康食品や医薬品原料として重要な脂肪酸である。しかしながら、ゼニゴケにおけるこれらの脂肪酸含有量は、僅かである。このため、これらの脂肪酸含有量を増やすことが、望まれる。このために、本発明者は人工光源を用いることを思いついた。   Zenigoke contains long-chain polyunsaturated fatty acids such as arachidonic acid and eicosapentaenoic acid that higher plants do not have. These fatty acids are important fatty acids for health foods and pharmaceutical raw materials. However, the content of these fatty acids in the moss is negligible. For this reason, it is desired to increase the content of these fatty acids. For this purpose, the inventor has come up with the use of an artificial light source.

人工光源を用いる栽培方法としては、照射光の波長分布を調整して植物体の栄養成分の含有量を調整するものがある（特許文献１）。   As a cultivation method using an artificial light source, there is a method of adjusting the content of nutrient components in a plant by adjusting the wavelength distribution of irradiation light (Patent Document 1).

なお、本発明者は、本発明を完成させる過程で、Ｍ５１Ｃと言われる寒天濃度1.4％の無菌栄養培地を直径９cmのシャーレに敷き、その上にゼニゴケの無性芽を置く生育条件を用いた（非特許文献１）。また、脂肪酸の抽出方法としては、非特許文献２の方法を用いた。   In addition, in the process of completing the present invention, the present inventor used a growth condition in which a sterile nutrient medium having an agar concentration of 1.4%, called M51C, was laid on a petri dish having a diameter of 9 cm and an asexual shoot of genus Agaricum was placed thereon. (Non-Patent Document 1). Moreover, the method of the nonpatent literature 2 was used as the extraction method of a fatty acid.

特開平８−２０５６７７号公報JP-A-8-205777

Takenaka, M., Yamaoka, S.,Hanajiri, T., Shimizu-Ueda, Y.,Yamato, K.T., Fukuzawa, H. and Ohyama,K. 2000. Direct transformation and plant regeneration of the haploid liverwortMarchantia polymorpha L. Transgen. Res. 9:179-185.Takenaka, M., Yamaoka, S., Hanajiri, T., Shimizu-Ueda, Y., Yamato, KT, Fukuzawa, H. and Ohyama, K. 2000.Direct transformation and plant regeneration of the haploid liverwortMarchantia polymorpha L. Transgen Res. 9: 179-185. Hamada, T., Iba, K., Shimada,T. 2006. Reduction of trienoicfatty acid content by expression of adouble-stranded RNA of a plastid W-3 fattyacid desaturase gene in transgenictobacco. Biotechnol. Lett. 28: 779-785.Hamada, T., Iba, K., Shimada, T. 2006. Reduction of trienoicfatty acid content by expression of adouble-stranded RNA of a plastid W-3 fattyacid desaturase gene in transgenictobacco. Biotechnol. Lett. 28: 779-785.

しかしながら、上述の特許文献１の栽培方法は、コマツナやレタス等を栽培対象としており、しかも、調整（増量）させる栄養成分として糖質、ビタミン等の含有量に着目したものであって、本発明とは栽培対象及び栄養成分が全く異なるものである。   However, the cultivation method of Patent Document 1 described above is intended for cultivation of komatsuna, lettuce and the like, and pays attention to the content of carbohydrates, vitamins and the like as nutritional components to be adjusted (increased). Is completely different in cultivated object and nutritional components.

本発明は上記実情を考慮して創作されたもので、その解決課題はゼニゴケのアラキドン酸及びエイコサペンタエン酸の含有量を増やすことである。   The present invention has been created in view of the above circumstances, and the problem to be solved is to increase the contents of arachidonic acid and eicosapentaenoic acid in the moss.

本発明の長鎖多不飽和脂肪酸の生産方法は、ゼニゴケに青色光を照射して栽培することを特徴とする。   The method for producing a long-chain polyunsaturated fatty acid according to the present invention is characterized in that it is cultivated by irradiating blue moss with blue light.

実験結果より、青色光が他の色よりもゼニゴケのアラキドン酸やエイコサペンタエン酸の含有量を、増やすことが証明された。   From the experimental results, it was proved that blue light increases the content of arachidonic acid and eicosapentaenoic acid in the genus Agaricosa than other colors.

各光質条件で培養したゼニゴケの総脂肪酸の含有量を質量比で示すグラフである。It is a graph which shows content of the total fatty acid of the mushrooms cultured on each light quality condition by mass ratio. 各光質条件で培養したゼニゴケのアラキドン酸、エイコサペンタエン酸の含有量を質量比で示すグラフである。It is a graph which shows the content of the arachidonic acid and eicosapentaenoic acid of the mushrooms cultured on each light quality condition by mass ratio.

６〜１２個のゼニゴケの無性芽（ゲンマ）を無菌栄養寒天培地上に静置し（非特許文献２と同様）、２５℃、３０μmol photons m-2s-1の各波長特性のＬＥＤ光（単色光、及び混合色光（表１参照））を連続照射する条件化で、５週間培養した。

Figure 2011041539
6-12 agaric buds (Gemma) are left still on a sterile nutrient agar medium (same as non-patent document 2), and LED light with each wavelength characteristic of 25 ° C. and 30 μmol photons m −2 s −1 The cells were cultured for 5 weeks under conditions of continuous irradiation with monochromatic light and mixed color light (see Table 1).
Figure 2011041539

５週間培養したゼニゴケについて、個々の脂肪酸を測定した。総脂肪酸の抽出は、非特許文献２の方法に準じて行った。抽出した総脂肪酸をガスクロマトグラフィーにより測定した。標準物質としてペンタデカン酸メチルエステル（15:0-Me）を使用した。その測定結果が、各光質における総脂肪酸の質量比として、図１のグラフに示してある。青色光では、他の波長のＬＥＤ光と比較して、ヘキサデカトリエン酸（16：3）及びα‐リノレン酸（18：3n-3）の減少、パルミチン酸（16：0）、アラキドン酸（20：4n-6）及びエイコサペンタエン酸（20：5n-3）の増加が見られる。図２のグラフに拡大して示すように、青色光で栽培したゼニゴケのアラキドン酸（20：4n-6）及びエイコサペンタエン酸（20：5n-3）の脂肪酸含有量は、他の波長のＬＥＤ光で栽培したものと比較して、それぞれ１及び２〜３％程度は増加している。同様の実験を４回行ったところ、全て同様の結果が得られた。   Individual fatty acids were measured on the genus C. moss cultured for 5 weeks. Extraction of total fatty acids was performed according to the method of Non-Patent Document 2. The extracted total fatty acid was measured by gas chromatography. Pentadecanoic acid methyl ester (15: 0-Me) was used as a standard substance. The measurement results are shown in the graph of FIG. 1 as the mass ratio of total fatty acids in each light quality. In blue light, compared with LED light of other wavelengths, decrease in hexadecatrienoic acid (16: 3) and α-linolenic acid (18: 3n-3), palmitic acid (16: 0), arachidonic acid ( 20: 4n-6) and eicosapentaenoic acid (20: 5n-3) increase. As shown in the enlarged graph of FIG. 2, the fatty acid content of arachidonic acid (20: 4n-6) and eicosapentaenoic acid (20: 5n-3) of the genus Coriella cultivated with blue light is different from that of other wavelengths. Compared with what was cultivated with light, each increased by about 1 and 2-3%. When the same experiment was performed four times, the same result was obtained in all cases.

以上の結果から青色ＬＥＤ光によるゼニゴケの栽培方法は、アラキドン酸（20：4n-6）及びエイコサペンタエン酸（20：5n-3）の脂肪酸含有量を増加させるには有効であることが明らかとなった。   From the above results, it is clear that the method of cultivating the genus Amanita using blue LED light is effective in increasing the fatty acid content of arachidonic acid (20: 4n-6) and eicosapentaenoic acid (20: 5n-3). became.

Claims (1)

ゼニゴケに青色光を照射して栽培することを特徴とするゼニゴケの長鎖多不飽和脂肪酸の生産方法。 A method for producing a long-chain polyunsaturated fatty acid of the moss, which is cultivated by irradiating the moss with blue light.
JP2009192962A 2009-08-24 2009-08-24 Production method of long chain polyunsaturated fatty acids Expired - Fee Related JP5374710B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2009192962A JP5374710B2 (en) 2009-08-24 2009-08-24 Production method of long chain polyunsaturated fatty acids

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2009192962A JP5374710B2 (en) 2009-08-24 2009-08-24 Production method of long chain polyunsaturated fatty acids

Publications (2)

Publication Number Publication Date
JP2011041539A true JP2011041539A (en) 2011-03-03
JP5374710B2 JP5374710B2 (en) 2013-12-25

Family

ID=43829459

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2009192962A Expired - Fee Related JP5374710B2 (en) 2009-08-24 2009-08-24 Production method of long chain polyunsaturated fatty acids

Country Status (1)

Country Link
JP (1) JP5374710B2 (en)

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63233783A (en) * 1987-03-23 1988-09-29 Mitsubishi Gas Chem Co Inc Method for culturing cells of bryophyte
JPH05111384A (en) * 1990-01-17 1993-05-07 Soc Prod Nestle Sa Production of polyvalent unsaturated fatty acid
JPH08205677A (en) * 1995-01-31 1996-08-13 Mitsubishi Chem Corp Regulation of nutrient ingredient content of plant body
JP2001258389A (en) * 2000-03-17 2001-09-25 Mitsubishi Chemicals Corp Method for cultivating plant
JP3094466U (en) * 2002-12-03 2003-06-20 藤崎電機株式会社 Crop cultivation device in agricultural house
WO2005061713A1 (en) * 2003-12-22 2005-07-07 Suntory Limited Unsaturated fatty acid synthase gene originating in marchantiales plant and utilization of the same
JP2007202471A (en) * 2006-02-02 2007-08-16 Hero:Kk Method for producing moss

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63233783A (en) * 1987-03-23 1988-09-29 Mitsubishi Gas Chem Co Inc Method for culturing cells of bryophyte
JPH05111384A (en) * 1990-01-17 1993-05-07 Soc Prod Nestle Sa Production of polyvalent unsaturated fatty acid
JPH08205677A (en) * 1995-01-31 1996-08-13 Mitsubishi Chem Corp Regulation of nutrient ingredient content of plant body
JP2001258389A (en) * 2000-03-17 2001-09-25 Mitsubishi Chemicals Corp Method for cultivating plant
JP3094466U (en) * 2002-12-03 2003-06-20 藤崎電機株式会社 Crop cultivation device in agricultural house
WO2005061713A1 (en) * 2003-12-22 2005-07-07 Suntory Limited Unsaturated fatty acid synthase gene originating in marchantiales plant and utilization of the same
JP2007202471A (en) * 2006-02-02 2007-08-16 Hero:Kk Method for producing moss

Also Published As

Publication number Publication date
JP5374710B2 (en) 2013-12-25

Similar Documents

Publication Publication Date Title
Qiao et al. Effect of culture conditions on growth, fatty acid composition and DHA/EPA ratio of Phaeodactylum tricornutum
Adarme-Vega et al. Towards sustainable sources for omega-3 fatty acids production
Ra et al. Effects of light-emitting diode (LED) with a mixture of wavelengths on the growth and lipid content of microalgae
Sahin et al. Variation in growth, fucoxanthin, fatty acids profile and lipid content of marine diatoms Nitzschia sp. and Nanofrustulum shiloi in response to nitrogen and iron
Khozin-Goldberg et al. LC-PUFA from photosynthetic microalgae: occurrence, biosynthesis, and prospects in biotechnology
ES2653727T3 (en) Desaturases and process for the production of polyunsaturated fatty acids in transgenic organisms
Chen et al. Enhancing the production of eicosapentaenoic acid (EPA) from Nannochloropsis oceanica CY2 using innovative photobioreactors with optimal light source arrangements
CN1662642A (en) Pasteurisation process for microbial cells and microbial oil
Che et al. Optimization of light intensity and photoperiod for Isochrysis galbana culture to improve the biomass and lipid production using 14-L photobioreactors with mixed light emitting diodes (LEDs) wavelength under two-phase culture system
WO1999064614A3 (en) Polyunsaturated fatty acids in plants
WO1999064616A3 (en) Polyunsaturated fatty acids in plants
Hu et al. Effects of nitrogen and phosphorous stress on the formation of high value LC-PUFAs in Porphyridium cruentum
RU2011108981A (en) OIL CONTAINING ONE OR MORE LONG LENGTH POLYESATURATED FATTY ACIDS IN THE FORM OF PHOSPHOLIPIDS PRODUCED FROM BIOMASS
Archer et al. Fatty acid profiling of new Irish microalgal isolates producing the high-value metabolites EPA and DHA
Adenan et al. Enhancement of lipid production in two marine microalgae under different levels of nitrogen and phosphorus deficiency
CN104757328B (en) A kind of compound living body bait of microalgae for fish hatching and its production method
KR101129716B1 (en) Method for production of specific fatty acid and lipid from microalgae using light from light emitting diodes
Bhakar et al. Total lipids and fatty acid profile of different Spirulina strains as affected by salinity and incubation time
JP5374710B2 (en) Production method of long chain polyunsaturated fatty acids
Liang et al. Differences in growth, total lipid content and fatty acid composition among 60 clones of Cylindrotheca fusiformis
IL262125B2 (en) Selective bioreactor for microalgae
Yago et al. Effect of wavelength of intermittent light on the growth and fatty acid profile of the haptophyte Isochrysis galbana
Mane et al. Amelioration for oxidative stability and bioavailability of N-3 PUFA enriched microalgae oil: an overview
JP2017163878A (en) Culture apparatus for eustigmatophyceae and culture method eustigmatophyceae
US20220010297A1 (en) Systems and methods for bleaching microbial cells

Legal Events

Date Code Title Description
A711 Notification of change in applicant

Free format text: JAPANESE INTERMEDIATE CODE: A711

Effective date: 20110929

RD02 Notification of acceptance of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7422

Effective date: 20110929

A521 Written amendment

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20110929

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20120705

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20130318

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20130806

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20130830

R150 Certificate of patent or registration of utility model

Free format text: JAPANESE INTERMEDIATE CODE: R150

LAPS Cancellation because of no payment of annual fees