JP2017163878A - Culture apparatus for eustigmatophyceae and culture method eustigmatophyceae - Google Patents

Abstract

PROBLEM TO BE SOLVED: To inhibit the growth of Eustigmatophyceae during cultivation.SOLUTION: The Eustigmatophyceae culture apparatus 100 is provided with a plurality of LED units 6. The LED units 6 are configured to use a plurality of inhibition LEDs which irradiate light with a center wavelength of 630 nm. The LED units 6 of the Eustigmatophyceae culture apparatus 100 are arranged around the edge of an aquarium 101 oriented so the front faces thereof face the aquarium 101. By exposing a culture 10 in the aquarium 101 to sunlight, growth of Nannochloropsis oculata 13 is enhanced in the culture 10. When the LED units 6 of the Eustigmatophyceae culture apparatus 100 are operated, light from the LEDs of the LED units 6 irradiate the Nannochloropsis oculata 13 in the culture 10.SELECTED DRAWING: Figure 3

Description

  The present invention relates to a true eye point algae culture apparatus and a true eye point algae culture method.

  In the seed production of marine fish, true-point algae such as Nannochloropsis oculata are used as useful microalgae. For example, Nannochloropsis oculata contains a large amount of highly unsaturated fatty acids such as EPA (eicosapentaenoic acid), so it can be used for feed or fortification of marine worms that are feed for marine larvae It is put into the aquarium containing marine larvae and marine aphids. Marine larvae that prey on Nannochloropsis oculata by the food chain are preyed on by marine larvae, so highly unsaturated fatty acids are accumulated in marine larvae and can produce marine larvae with high nutritional value. In addition, Nannochloropsis oculata has the property of absorbing ammonia nitrogen and therefore exhibits the effect of maintaining good water quality during seedling production. Furthermore, since Nannochloropsis oculata contains chloroplasts, the inside of the aquarium is made green, and the effect of calming the behavior of marine larvae and so-called green water effects is exhibited.

  As a method for culturing a large amount of a culture object such as true eye point algae such as Nannochloropsis oculata, there is a method of placing the culture object in a water tank installed outdoors and irradiating the culture object with sunlight. However, there is a limit to the sunshine time of the day, and there is a problem that the culturing efficiency of the culture object is not stable in the above method because the sunshine may be insufficient due to bad weather or the like. When trying to cultivate the culture object outdoors, there is a problem that microorganisms other than the culture object, for example, cyanobacteria and the like are mixed and proliferated, and as a result, the culture efficiency of the culture object decreases. In addition, when the amount of light irradiated to the culture object increases, such as under the hot sun in summer, there is a problem that the growth is accelerated and the culture object is withered due to lack of nutrition. In order to solve such a problem, there is a method in which a culture object is placed in a water tank installed indoors and the culture object is irradiated with light such as a fluorescent lamp or LED (for example, Patent Document 1).

  Patent Document 1 discloses a method of using an LED as a light source and irradiating a photosynthetic organism (cultured object) with light having a peak wavelength of about 540 nm or less.

JP 2004-147461 A

  However, in the method disclosed in Patent Document 1, since the growth of the culture target cannot be suppressed, there is still a problem that the culture target is still withered if the amount of light is large.

  The present invention has been made in view of the above circumstances, and provides a true eye-point algae culture device and a true eye-point algae culture method capable of suppressing the growth of true eye-point algae during culture. Objective.

  The inventor conducted extensive research on the absorbance characteristics of true-eye point algae. As a result, the inventor obtained new knowledge about light having a wavelength that suppresses the growth of true-eye point algae, and completed the present invention.

In order to achieve the above object, a true eye-point algae culture device according to the first aspect of the present invention comprises:
It comprises a suppression LED that irradiates light of a wavelength that suppresses the growth of the true eyed point algae against the true eyed point algae in culture,
It is characterized by that.

In the above-described true eye point algae culture apparatus,
The true eye point algae is Nannochloropsis oculata,
You may do it.

In the above-described true eye point algae culture apparatus,
The suppression LED irradiates light having a center wavelength of 610 to 650 nm.
You may do it.

In the above-described true eye point algae culture apparatus,
Further comprising a promoting LED that irradiates the true eye point algae with light of a wavelength that promotes the growth of the true eye point algae,
Irradiating simultaneously the light of the LED for promotion and the light of the LED for suppression,
You may do it.

In the above-described true eye point algae culture apparatus,
The LED for promotion irradiates at least one of light having a center wavelength of 410 to 470 nm and light having a center wavelength of 660 to 690 nm.
You may do it.

In the above-described true eye point algae culture apparatus,
The ratio of the light quantity of the promotion LED and the light quantity of the suppression LED is 2: 1.
You may do it.

In the above-described true eye point algae culture apparatus,
Further comprising a carotenoid LED that emits light having a central wavelength of 480 to 520 nm with respect to the true eye-point algae,
Irradiating at least the light of the suppression LED and the light of the carotenoid LED simultaneously,
You may do it.

In order to achieve the above object, a method for cultivating true eye point algae according to the second aspect of the present invention includes:
Irradiating light of a wavelength that suppresses the growth of the true eye point algae to the true eye point algae in culture,
It is characterized by that.

  According to the present invention, it is possible to suppress the growth of true eye point algae during culture.

1 is a perspective view of a true eye point algae culture device according to Embodiment 1. FIG. FIG. 2 is a side view of the true eye point algae culture apparatus of FIG. 1. 5 is a perspective view of a true eye point algae culture device according to Embodiment 2. FIG. It is a figure which shows a proliferation characteristic. It is a figure which shows an optical density characteristic. It is a figure which shows the growth characteristic in different irradiation conditions. It is a figure which shows the amount of chlorophyll per cell.

  Embodiments according to the present invention will be described with reference to the drawings. In addition, this invention is not limited by the following embodiment and drawing.

(Embodiment 1)
First, the first embodiment according to the present invention will be described in detail. FIG. 1 is a perspective view of a true eye-point algae culture apparatus 1 according to the first embodiment. FIG. 2 is a side view of the true eye point algae culture apparatus 1 of FIG. The true eye-point algae culture apparatus 1 according to Embodiment 1 is an apparatus used indoors as an example. As shown in FIGS. 1 and 2, the true eye point algae culture apparatus 1 includes a table 2, LED (light emitting diode) units 3 to 6, and an air supply unit 7. The true eye point algae culture apparatus 1 may not include the table 2 or the air supply unit 7.

  The table 2 has a size that allows the container 11 containing the culture strain 10 to be placed thereon. The culture strain 10 is prepared from a culture solution 12 and a small amount of Nannochloropsis oculata 13. The container 11 is made of, for example, transparent glass, and transmits light incident from the LED units 3 to 6. The LED units 3 to 6 are installed on the edge of the table 2 so that the front faces the container 11 placed on the table 2. Therefore, the light from the LED units 3 to 6 is applied to the nannochloropsis occulter 13 in the container 11 placed on the table 2. Each of the LED units 3 to 6 includes, for example, a plurality of LEDs (also referred to as LED elements). In addition, each LED unit 3-6 may be provided with one LED.

  In the first embodiment, nannochloropsis oculata belonging to the genus Nannochloropsis, which is a true eye-point algae, is the subject of culture. Not. For example, the culture object may be Nannochloropsis salina of the genus Nannochloropsis.

  As an example, the LED units 3 and 4 are configured using a plurality of LEDs 3a and 4a that emit light having a central wavelength of 455 nm. The wavelength of 455 nm is a wavelength that promotes the growth of Nannochloropsis oculata. The LEDs 3a and 4a constituting the LED units 3 and 4 are also referred to as promotion LEDs. “Proliferation of nannochloropsis oculata” means that the nannochloropsis oculata performs photosynthesis, specifically, the chlorophyll (chlorophyll a) possessed by the nonnochloropsis oculata absorbs light and performs photosynthesis. This means that the number of Nannochloropsis oculata increases after cell division. In addition, the wavelength which accelerates | stimulates the proliferation of Nannochloropsis oculata is not limited to 455 nm, What is necessary is just 410-470 nm or 660-690 nm. Therefore, LED3a, 4a which comprises LED unit 3, 4 should just irradiate at least any one of the light whose center wavelength is 410-470 nm and the center wavelength is 660-690 nm, It is not limited to what irradiates light with a central wavelength of 455 nm. For example, the LED 3a may emit light having a central wavelength of 455 nm, and the LED 4a may emit light having a central wavelength of 680 nm. Alternatively, the LED 3a may emit light having a central wavelength of 455 nm, and the LED 4a may emit light having a central wavelength of 410 nm. Since light with a wavelength of 410 to 470 nm appears blue, an LED that emits light with this wavelength is also referred to as a blue LED. In addition, since light with a wavelength of 660 to 690 nm looks red, an LED that emits light with this wavelength is also referred to as a red LED.

  As an example, the LED unit 5 includes a plurality of LEDs 5a that emit light having a center wavelength of 520 nm. The wavelength of 520 nm is a wavelength at which Nannochloropsis oculata efficiently produces carotenoids in the cell. The LED 5a constituting the LED unit 5 is also referred to as a carotenoid LED. The wavelength at which Nannochloropsis oculata efficiently produces carotenoids is not limited to 520 nm, and may be 480 to 520 nm. Therefore, LED5a which comprises LED unit 5 should just irradiate the light whose center wavelength is 480-520 nm, and is not limited to what irradiates the light whose center wavelength is 520 nm. For example, the LED 5a may emit light having a center wavelength of 500 nm. Since light with a wavelength of 480 to 520 nm looks green (blue-green), an LED that emits light with this wavelength is also referred to as a green LED (blue-green LED).

  As an example, the LED unit 6 includes a plurality of LEDs 6a that emit light having a center wavelength of 630 nm. The wavelength of 630 nm is a wavelength that suppresses the growth of Nannochloropsis oculata. The LED 6a constituting the LED unit 6 is also referred to as a suppression LED. The wavelength for suppressing the growth of Nannochloropsis oculata is not limited to 630 nm, and may be 610 to 650 nm. Therefore, LED6a which comprises LED unit 6 should just irradiate the light whose center wavelength is 610-650 nm, and is not limited to what irradiates the light whose center wavelength is 630 nm. For example, the LED 6a may emit light having a center wavelength of 620 nm. Since light with a wavelength of 610 to 650 nm appears red, an LED that emits light with this wavelength is also referred to as a red LED.

  As shown in FIG. 2, the air supply unit 7 includes an air pump (not shown), a vent pipe 8 whose base end is connected to the air pump, and an air stone 9 connected to the tip of the vent pipe 8. .

  Next, the operation of the true eye point algae culture apparatus 1 will be described.

  In order to culture the nannochloropsis oculata, first, the culture solution 10 and a small amount of the nannochloropsis oculata 13 are put into the container 11 to prepare a culture strain 10 having a predetermined concentration. Then, the container 11 containing the prepared culture strain 10 is placed on the table 2 of the true eye point algae culture apparatus 1. Next, the air stone 9 is put into the culture strain 10 and the air pump is started. As a result, air necessary for the photosynthesis of the Nannochloropsis occulta 13 passes through the vent tube 8 and the air stone 9 and is supplied into the culture strain 10. The container 11 may be covered so that dust and microorganisms other than the Nannochloropsis oculata 13 are not mixed.

  Next, when the LED units 3 to 6 are operated, the light of each wavelength is irradiated to the container 11 from the LEDs 3a, 4a, 5a, and 6a of the LED units 3 to 6 as indicated by arrows in FIG. As described above, since the container 11 transmits incident light, the light from the LED units 3 to 6 is applied to the nannochloropsis oculata 13 in the culture strain 10.

  As described above, the LEDs 3a and 4a constituting the LED units 3 and 4 emit light having a central wavelength of 455 nm. Accordingly, the Nannochloropsis occulta 13 is irradiated with light having a wavelength of 455 nm. Thereby, the proliferation of Nannochloropsis oculata 13 is promoted. Moreover, LED6a which comprises LED unit 6 irradiates light with a center wavelength of 630 nm. Therefore, the nannochloropsis occulta 13 is irradiated with light having a wavelength of 630 nm. Thereby, the proliferation of Nannochloropsis oculata 13 is suppressed. Furthermore, LED5a which comprises LED unit 5 irradiates light with a center wavelength of 520 nm. Accordingly, the Nannochloropsis occulta 13 is irradiated with light having a wavelength of 520 nm. As a result, carotenoids are efficiently produced in the cells of Nannochloropsis oculata 13.

  In this embodiment, LEDs 3a and 4a shown in FIG. 1 are used as LEDs for promoting irradiation with light having a wavelength that promotes the proliferation of Nannochloropsis oculata. Moreover, LED6a shown in FIG. 1 is used as LED for suppression which irradiates the light of the wavelength which suppresses the proliferation of Nannochloropsis oculata. One LED unit 3 including the LED 3a, one LED unit 4 including the LED 4a, and one LED unit 6 including the LED 6a are included. With such a configuration, the ratio between the light amount of the promotion LED and the light amount of the suppression LED is 2: 1.

  As described above, the true eye-point algae culture apparatus 1 according to the first embodiment includes a suppression LED (LED 6a) that irradiates light with a wavelength of 630 nm that suppresses the growth of Nannochloropsis oculata, and Nannochloro. It comprises a promotion LED (LEDs 3a, 4a) that irradiates light with a wavelength of 455 nm that promotes the growth of psioculus, and a carotenoid LED (LED 5a) that emits light with a wavelength of 520 nm. On the other hand, the light of the LED for suppression, the light of the LED for promotion, and the light of the LED for carotenoid are irradiated simultaneously. Further, the ratio of the light quantity of the promotion LED and the light quantity of the suppression LED is 2: 1. As a result, the growth rate of Nannochloropsis oculata can be adjusted to a suitable rate, and carotenoids can be produced efficiently.

  In the first embodiment, the true eye-point algae culture device 1 has been described as including the suppression LED (LED 6a), the promotion LED (LED 3a, 4a), and the carotenoid LED (LED 5a). However, the present invention is not limited to this. For example, the true eye point algae culture device 1 includes a suppression LED and a promotion LED, but may not include a carotenoid LED. Even in this case, the growth rate of the Nannochloropsis oculata can be adjusted to a suitable rate.

  In addition, for example, the true eye point algae culture device 1 includes the suppression LED, but may not include the promotion LED and the carotenoid LED. In this case, for example, Nannochloropsis oculata may be cultured in an environment where a fluorescent lamp is lit. For example, when the light from a fluorescent lamp is irradiated onto the nannochloropsis occulta, the nannochloropsis oculiata synthesizes and proliferates. When cultivating Nannochloropsis oculata in this way, by operating the LED for suppression of the true eye-point algae culture apparatus 1 and irradiating the Nannochloropsis oculata with the light of the LED for suppression, The growth of Nannochloropsis oculata during culture can be suppressed. For example, when the amount of light from a fluorescent lamp increases, the growth of Nannochloropsis oculata accelerates, and the Nannochloropsis oculata may wither for reasons such as lack of nutrition. By using the culture apparatus 1, the growth of Nannochloropsis oculata can be suppressed. Therefore, it is possible to prevent the Nannochloropsis oculata from withering.

  In addition, for example, the true eye-point algae culture apparatus 1 includes a suppression LED and a carotenoid LED, but may not include a promotion LED. In this case, for example, when the nannochloropsis oculata is cultured in an environment in which a fluorescent lamp is lit as described above, the true eye point algae culture apparatus 1 may be used. As a result, the growth of Nannochloropsis oculata during culture can be suppressed, and carotenoids can be produced efficiently.

  In the first embodiment, the ratio of the light amount of the promotion LED and the light amount of the suppression LED is 2: 1. However, the present invention is not limited to this. For example, the ratio between the light amount of the promotion LED and the light amount of the suppression LED may be 1: 1, 1: 2, 3: 1, 4: 1, or the like. In this way, the growth rate of Nannochloropsis oculata can be set according to the culture environment.

  In the said Embodiment 1, as shown in FIG. 1, LED unit 3-6 was arrange | positioned. That is, LED units 3 to 6 are arranged in the order of LED unit 3 (blue), LED unit 4 (blue), LED unit 5 (green), and LED unit 6 (red) from the left in FIG. However, the present invention is not limited to this, and the arrangement of the LED units 3 to 6 may be changed. For example, LED units 3 to 6 may be arranged in the order of LED unit 3 (blue), LED unit 5 (green), LED unit 6 (red), and LED unit 4 (blue) from the left in FIG. Moreover, you may increase the number of each LED unit. For example, four LED units 3 and 4 (blue) in total, two LED units 6 (red) in total, two LED units 5 (green) in total (or one), and the like may be used.

(Embodiment 2)
Next, the second embodiment will be described in detail. FIG. 3 is a perspective view of the true eye point algae culture apparatus 100 according to the second embodiment. The true eye-point algae culture apparatus 100 according to the second embodiment is an apparatus used outdoors as an example. As shown in FIG. 3, the true eye-point algae culture apparatus 100 includes a plurality of LED units 6 and an air supply unit (not shown). The LED unit 6 in the second embodiment is the same as the LED unit 6 in the first embodiment. Therefore, the LED unit 6 is configured by using a plurality of suppression LEDs (not shown in FIG. 3) that emit light having a center wavelength of 630 nm.

  Next, the operation of the true eye point algae culture apparatus 100 will be described.

  In order to cultivate Nannochloropsis oculata, first, a culture solution 12 and a small amount of Nannochloropsis oculata 13 are placed in a water tank 101 installed outdoors to prepare a culture strain 10 having a predetermined concentration. To do. Then, the LED unit 6 of the true eye point algae culture device 100 is installed at the edge of the water tank 101 with the front face facing the water tank 101.

  As shown in FIG. 3, the sunlight 10 is irradiated to the culture strain 10 in the water tank 101, thereby promoting the growth of the Nannochloropsis oculata 13 in the culture strain 10. Here, when the LED unit 6 of the true eye-point algae culture device 100 is operated, light from the LED of the LED unit 6 is irradiated to the nannochloropsis oculata 13 in the culture strain 10. As described above, the LED of the LED unit 6 emits light having a center wavelength of 630 nm. Therefore, the growth of Nannochloropsis oculata 13 is suppressed.

  As described above, the true eye-point algae culture apparatus 100 according to the second embodiment is a suppression LED that emits light having a wavelength of 630 nm that suppresses the growth of Nannochloropsis oculata (LED of the LED unit 6). The nannochloropsis oculata in culture is irradiated with the light of the suppression LED. Thereby, the growth of Nannochloropsis oculata can be suppressed. For example, when the amount of light radiated from the sun to the nannochloropsis oculata increases, such as under the hot sun in the summer, the growth of the nannochloropsis occultata accelerates, and the nannochloropsis occulta withers due to lack of nutrition. However, the growth of Nannochloropsis oculata can be suppressed by using the true eye-point algae culture apparatus 100 according to the second embodiment. Therefore, it is possible to prevent the Nannochloropsis oculata from withering.

  In the second embodiment, the true eye-point algae culture apparatus 100 has been described as including a suppression LED. However, the present invention is not limited to this. For example, the true eye-point algae culture apparatus 100 includes a suppression LED and a promotion LED, a suppression LED and a carotenoid LED, a suppression LED, a promotion LED, and a carotenoid LED. It may be provided. For example, in FIG. 3, a part of the LED unit 6 is replaced with the LED unit 3 in the first embodiment, or the LED unit 3 in the first embodiment is added in addition to the LED unit 6. Thus, the true eye point algae culture device 100 may be configured to include at least a suppression LED and a promotion LED. With such a configuration, it is possible to culture Nannochloropsis oculata even at night, and the culture efficiency can be increased. Further, when the amount of light irradiated from the sun to the Nannochloropsis oculata is small, such as in winter, the amount of light necessary for the growth of the Nannochloropsis oculata can be supplemented by operating the promotion LED.

  The following examples further illustrate the present invention, but the present invention is not limited to the examples.

  First, in order to investigate the growth characteristics of Nannochloropsis oculata, cultures with different concentrations were prepared. As a method of creating a culture strain having different concentrations, for example, one culture strain having a predetermined concentration is prepared, and this is cultured for a predetermined number of days (for example, 3 days), and then divided into two. Create two. One of the two cultures A is stored in a cool dark place so that the Nannochloropsis oculata does not grow or is difficult to grow. Further, after culturing the other for a predetermined number of days (for example, 3 days), it is divided into two, and two second-stage cultures B are prepared. One of the two cultures B is stored in a cool dark place so that the Nannochloropsis oculata does not grow or is difficult to grow. The other is cultured for a predetermined number of days (for example, 3 days) to produce a third stage culture. By these methods, culture strains A, B, and C having different concentrations were prepared.

Then, Nannochloropsis oculata was cultured using different concentrations of the culture strains A, B, and C under the following culture conditions. As culture conditions, the water temperature of the culture solution was 20 ° C., and the salinity was 23%. The light intensity of the fluorescent lamp was set to 150 μmol photoms m ⁻² s ^−1, and the light from the fluorescent lamp was continuously irradiated. The medium was designated as f medium. And it culture | cultivated batch for 11 days in the state which always ventilated.

  In the experiment, the cell concentration CD for Nannochloropsis oculata was measured every day from the start of culture. The experimental results are shown in FIG. 4 as growth characteristics. As shown in FIG. 4, even if there is a great difference in the value of cell concentration CD depending on the culture strain at the start of the culture (culture strain A: about 11.5, culture strain B: about 15.5, culture strain C: About 16.7), it was confirmed that the difference narrowed as the number of days increased and finally converged to approximately the same value (about 18.8). From this, it can be said that the concentration of the finally obtained culture strain is almost the same no matter what concentration of culture strain is used to cultivate Nannochloropsis oculata.

  Moreover, in the said experiment, the optical density OD about Nannochloropsis oculata was also measured. The experimental results are shown in FIG. 5 as optical density characteristics. Since all the culture strains showed substantially the same optical density characteristics (absorbance characteristics), FIG. 5 shows the optical density characteristics for the culture strain A and omits the optical density characteristics for the culture strains B and C. As shown in FIG. 5, a region I where the optical density OD peaks in the wavelength range of 410 to 470 nm appears, a region II where the optical density OD peaks in the wavelength range of 480 to 520 nm appears, and the wavelength is 610. A region III where the optical density OD peaks in a range of ˜650 nm appears, and a region IV where the optical density OD peaks in a wavelength range of 660 to 690 nm appears. Of these regions, region I and region IV are wavelength regions that are absorbed by chlorophyll of the nannochloropsis oculata. Chlorophyll, also called chlorophyll, is activated by absorbing light energy and has the property of contributing to cell division. Accordingly, irradiation with light having a wavelength that activates chlorophyll promotes the growth of Nannochloropsis oculata. Region II is a wavelength region that is absorbed by the carotenoid of Nannochloropsis oculata. By irradiating with light having a wavelength in this region II, carotenoids are efficiently produced in the cells of Nannochloropsis oculata 13. Region III is a wavelength region that suppresses the growth of Nannochloropsis oculata. By irradiating with light having the wavelength of region III, the growth of Nannochloropsis oculata is suppressed.

Next, proliferation characteristics under different irradiation conditions were measured. In this experiment, Nannochloropsis oculata was cultured under the same conditions as those described above except that the light source was changed from a fluorescent lamp to an LED. That is, the water temperature of the culture solution was 20 ° C., and the salinity was 23%. The light intensity of the entire LED was set to 150 μmol photoms m ⁻² s ⁻¹ , and the LED light was continuously irradiated. The medium was designated as f medium. And it culture | cultivated batch for 11 days in the state which always ventilated. In the following description, the term “blue LED unit” refers to an LED unit (LED unit in the first embodiment) that includes an LED (the LED 3a or LED 4a in the first embodiment) that emits light having a central wavelength of 455 nm. 3 or LED unit 4), and the term “red LED unit” refers to an LED unit (in the first embodiment, the LED 6a in the first embodiment) that emits light having a center wavelength of 630 nm. LED unit 6) refers to an LED unit (LED unit in the first embodiment) including an LED (LED 5a in the first embodiment) that emits light having a central wavelength of 520 nm. It shall refer to 5).

  As irradiation conditions, Condition 1: When the light source is two blue LED units, one red LED unit and one green LED unit, Condition 2: When the light source is two blue LED units and two red LED units, Condition 3: An experiment was conducted in the case where the light source was one blue LED unit, two red LED units, and one green LED unit.

  The experimental results are shown in FIG. 6 as growth characteristics under different irradiation conditions. In order to compare the growth characteristics depending on the light source, FIG. 6A shows the growth characteristics when the light source is white light illumination (fluorescent lamp) (for the culture strain A in FIG. 4). 6B shows the experimental result under condition 1, FIG. 6C shows the experimental result under condition 2, and FIG. 6D shows the experimental result under condition 3. FIG. 6A to 6D, A indicates the logarithmic phase and B indicates the stationary phase (stationary phase).

  Comparing FIG. 6 (a) and FIG. 6 (b), it can be seen that the proliferation characteristics are hardly changed. Therefore, even when an LED is used instead of the fluorescent lamp as the light source, the nannochloropsis occulta can be cultured in the same manner as in the case where the nannochloropsis oculiata is cultured using the fluorescent lamp. Since the true eye-point algae culture apparatuses 1 and 100 in the first and second embodiments are configured using LEDs that consume less power than fluorescent lamps, the Nannochloropsis oculata is reduced while suppressing power consumption. It can be cultured.

  Comparing FIG. 6 (b) and FIG. 6 (c), it can be seen that the slope in the vicinity of the logarithmic period A is gentler in FIG. 6 (c) than in FIG. 6 (b). From this, it can be said that the proliferation of Nannochloropsis oculata is suppressed by increasing the number of red LED units. Moreover, it can be said that the growth of Nannochloropsis oculata is promoted by reducing the red LED units.

  Further, comparing FIG. 6B and FIG. 6D, it can be seen that the slope in the vicinity of the logarithmic period A is gentler in FIG. 6D than in FIG. 6B. From this, it can be said that the proliferation rate of Nannochloropsis ocularata is slowed by reducing the number of blue LED units and increasing the number of red LED units. In addition, it can be said that the proliferation rate of Nannochloropsis oculata increases by increasing the number of blue LED units and decreasing the number of red LED units. Thus, by using the blue LED unit and the red LED unit, the growth rate of the Nannochloropsis oculata can be controlled.

  Moreover, in the said conditions 1-3, the amount of chlorophyll per cell of Nannochloropsis oculata was measured. Experimental results are shown in FIGS. 7A to 7C as chlorophyll amount characteristics. 7A to 7C also show results when the temperature of the culture solution is set to 23 ° C. in addition to the case where the temperature of the culture solution is set to 20 ° C. Comparing FIG. 7 (a) and FIG. 7 (b), it can be seen that the amount of chlorophyll per cell of Nannochloropsis oculata is generally smaller in FIG. 7 (b) than in FIG. 7 (a). . From this, by increasing the number of red LED units, the amount of chlorophyll per cell of Nannochloropsis oculata decreases as a whole. That is, it can be said that the activation of chlorophyll is suppressed. Moreover, it can be said that the activation of chlorophyll is promoted by reducing the number of red LED units.

  Further, comparing FIG. 7 (a) and FIG. 7 (c), the amount of chlorophyll per cell of Nannochloropsis oculata is generally smaller in FIG. 7 (c) than in FIG. 7 (a). I understand. From this, it can be said that activation of chlorophyll can be suppressed by reducing the number of blue LED units and increasing the number of red LED units. Moreover, it can be said that activation of chlorophyll is promoted by increasing the number of blue LED units and decreasing the number of red LED units.

  The above-described embodiments are for explaining the present invention and do not limit the scope of the present invention. In other words, the scope of the present invention is shown not by the embodiments but by the claims. Various modifications within the scope of the claims and within the scope of the equivalent invention are considered to be within the scope of the present invention.

  The present invention is useful for culturing true-eye point algae.

DESCRIPTION OF SYMBOLS 1 True eye point algae culture apparatus 2 Table 3, 4, 5, 6 LED unit 3a, 4a LED (blue)
5a LED (green)
6a LED (red)
DESCRIPTION OF SYMBOLS 7 Air supply part 8 Vent pipe 9 Air stone 10 Culture strain 11 Container 12 Culture solution 13 Nannochloropsis oculata 100 True eye point algae culture apparatus 101 Water tank

Claims (8)

It comprises a suppression LED that irradiates light of a wavelength that suppresses the growth of the true eyed point algae against the true eyed point algae in culture,
A true eye-point algae culture apparatus. The true eye point algae is Nannochloropsis oculata,
The true eye point algae culture apparatus according to claim 1. The suppression LED irradiates light having a center wavelength of 610 to 650 nm.
The apparatus for cultivating true eye-point algae according to claim 1 or 2. Further comprising a promoting LED that irradiates the true eye point algae with light of a wavelength that promotes the growth of the true eye point algae,
Irradiating simultaneously the light of the LED for promotion and the light of the LED for suppression,
The apparatus for cultivating true-eyed point algae according to any one of claims 1 to 3, wherein: The LED for promotion irradiates at least one of light having a center wavelength of 410 to 470 nm and light having a center wavelength of 660 to 690 nm.
The true eye point algae culture apparatus according to claim 4. The ratio of the light quantity of the promotion LED and the light quantity of the suppression LED is 2: 1.
The true eye-point algae culture apparatus according to claim 4 or 5. Further comprising a carotenoid LED that emits light having a central wavelength of 480 to 520 nm with respect to the true eye-point algae,
Irradiating at least the light of the suppression LED and the light of the carotenoid LED simultaneously,
The true eye-point algae culture apparatus according to any one of claims 1 to 6, wherein Irradiating light of a wavelength that suppresses the growth of the true eye point algae to the true eye point algae in culture,
A method for culturing binocular algae characterized by the above.

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