JP2011036146A5

JP2011036146A5 -

Info

Publication number: JP2011036146A5
Application number: JP2009184167A
Authority: JP
Filing date: 2009-08-07
Publication date: 2012-08-23
Anticipated expiration: 2029-08-07

Description

すなわち、本発明は、以下のいずれかの構成を特徴とするものである。
（１）培養反応槽に貯留されている微生物もしくは培養細胞の培養液を連続的に、かつ循環量を変化させながら分離膜へ供給循環し、培養によって生産された化学品を含む濾液を分離膜の透過側で回収するとともに、前記分離膜で濾過されなかった未濾過培養液を前記培養反応槽へ還流して連続培養を行うに際し、培養反応槽内の酸素移動容量係数ｋＬａの設定値からの減少率を該設定値の３０％以内に抑制することを特徴とする連続培養による化学品の製造方法。
（２）前記未濾過培養液を前記培養反応槽へ還流するに際し、前記未濾過培養液は、前記培養反応槽に貯留されている培養液中に直接還流させる、前記（１）に記載の連続培養による化学品の製造方法。
（３）前記設定値が７．０であり、前記化学品としてＬ−乳酸またはＤ−乳酸を回収する、前記（１）または（２）に記載の連続培養による化学品の製造方法。 That is, the present invention is characterized by any one of the following configurations.
(1) A culture solution of microorganisms or cultured cells stored in a culture reaction tank is continuously supplied to a separation membrane while changing the circulation rate, and the filtrate containing a chemical product produced by the culture is separated. From the set value of the oxygen transfer capacity coefficient kLa in the culture reaction tank when continuous culture is conducted by refluxing the unfiltered culture solution not filtered by the separation membrane to the culture reaction tank while recovering on the permeation side of A method for producing a chemical product by continuous culture, which comprises suppressing the reduction rate to within 30% of the set value.
(2) When refluxing the unfiltered culture solution to the culture reaction tank, the unfiltered culture solution is directly refluxed to the culture solution stored in the culture reaction tank, according to (1), Method of producing chemical products by culture.
(3) The method for producing a chemical according to (1) or (2), wherein the set value is 7.0 and L-lactic acid or D-lactic acid is recovered as the chemical .

Claims

The culture solution of microorganisms or cultured cells stored in the culture reaction tank is continuously supplied to the separation membrane while changing the amount of circulation, and the filtrate containing the chemicals produced by the culture is the permeation side of the separation membrane. When carrying out continuous culture by refluxing the unfiltered culture solution not filtered by the separation membrane to the culture reaction tank, the reduction rate of oxygen transfer capacity coefficient kLa in the culture reaction tank from the set value is calculated. A method for producing a chemical product by continuous culture, which comprises suppressing within 30% of the set value.

The chemical product according to claim 1, wherein the unfiltered culture solution is directly refluxed into the culture solution stored in the culture reaction tank when the unfiltered culture solution is refluxed to the culture reaction tank. Manufacturing method.

The method for producing a chemical product by continuous culture according to claim 1 or 2, wherein the set value is 7.0, and L-lactic acid or D-lactic acid is recovered as the chemical product.