JP2011033556A5 - - Google Patents

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Publication number
JP2011033556A5
JP2011033556A5 JP2009182075A JP2009182075A JP2011033556A5 JP 2011033556 A5 JP2011033556 A5 JP 2011033556A5 JP 2009182075 A JP2009182075 A JP 2009182075A JP 2009182075 A JP2009182075 A JP 2009182075A JP 2011033556 A5 JP2011033556 A5 JP 2011033556A5
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Japan
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mobile phase
sample solution
column
injector
analysis
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JP2009182075A
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Japanese (ja)
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JP5195686B2 (en
JP2011033556A (en
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上述のように２次元目の分析スケジュールが設定された後に２次元目のＬＣ分析が開始されると、制御・処理部２０の制御の下に、６方バルブ３が図１中の点線で示す接続状態に切り替えられ、第２送液ポンプ９は第２移動相容器８から移動相を吸引し、インジェクタ５を介して第２カラム１０に略一定流量でその移動相を送給する。オートサンプラ４は分析スケジュールに従った試料液を規定量用意（図５（ｃ）の例の場合、まずバイアル番号「１０１」のバイアル中の試料液を１．５ｍＬ用意）し、所定のタイミングでインジェクタ５が移動相中に試料液を注入する。注入された試料液は移動相の流れに乗って第２カラム１０に導入され、第２カラム１０を通過する過程で試料液中の各種成分が分離される。 When the second-dimensional LC analysis is started after the second-dimensional analysis schedule is set as described above, the six-way valve 3 is indicated by a dotted line in FIG. 1 under the control of the control / processing unit 20. When switched to the connected state, the second liquid feeding pump 9 sucks the mobile phase from the second mobile phase container 8 and feeds the mobile phase to the second column 10 through the injector 5 at a substantially constant flow rate. The autosampler 4 prepares a specified amount of sample solution according to the analysis schedule (in the case of FIG. 5C, first prepare 1.5 mL of the sample solution in the vial with the vial number “101”), The injector 5 injects a sample solution into the mobile phase. The injected sample solution is introduced into the second column 10 along the flow of the mobile phase, and various components in the sample solution are separated in the process of passing through the second column 10.

JP2009182075A 2009-08-05 2009-08-05 Liquid chromatograph Active JP5195686B2 (en)

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JP2009182075A JP5195686B2 (en) 2009-08-05 2009-08-05 Liquid chromatograph

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JP2009182075A JP5195686B2 (en) 2009-08-05 2009-08-05 Liquid chromatograph

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JP2011033556A JP2011033556A (en) 2011-02-17
JP2011033556A5 true JP2011033556A5 (en) 2012-01-19
JP5195686B2 JP5195686B2 (en) 2013-05-08

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6558032B2 (en) * 2015-04-03 2019-08-14 株式会社島津製作所 Preparative chromatograph
WO2022190457A1 (en) * 2021-03-09 2022-09-15 株式会社島津製作所 Preparative liquid chromatograph
WO2023105997A1 (en) * 2021-12-10 2023-06-15 株式会社島津製作所 Preparative liquid chromatograph and control method therefor

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH02150768A (en) * 1988-11-30 1990-06-11 Fuji Chem Kk Liquid chromatograph analysis apparatus
US6802967B2 (en) * 2002-03-06 2004-10-12 Shimadzu Corporation Multi-dimension liquid chromatography separation system
JP4447851B2 (en) * 2003-05-29 2010-04-07 株式会社島津製作所 Liquid chromatograph
TW200626570A (en) * 2004-11-29 2006-08-01 Suntory Ltd Intraoral pungent substance
JP2007057526A (en) * 2005-07-26 2007-03-08 Showa Denko Kk Method for analyzing low-molecular-weight compound in sample containing water-soluble polymer and low-molecular-weight compound

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