JP2011026279A - Hair wash using biosurfactant - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a hair wash comprising a biosurfactant so as to protect hair from physical and chemical stimulations and impart favorable feelings such as smoothness, moistness and flexibility inherent in healthy hair to hair. <P>SOLUTION: The hair wash comprises (A) the biosurfactant, (B) an anionic surfactant and (C) an organic acid, an inorganic acid or salt thereof. The hair wash can protect hair from physical and chemical stimulations and impart smoothness, moistness, flexibility inherent in healthy hair, etc., to hair. <P>COPYRIGHT: (C)2011,JPO&INPIT

Description

The present invention relates to a hair cleanser that utilizes the self-accumulation ability of a biosurfactant. More specifically, it is a mannosyl erythritol lipid (hereinafter sometimes simply referred to as MEL) which is a kind of glycolipid produced by microorganisms, and the mannosyl erythritol skeleton has 4-O-β-D-mannopyranosyl- Using mannosyl erythritol lipid which is meso-erythritol or 1-O-β-D-mannopyranosyl-meso-erythritol, the hair is protected from physical and chemical irritation without degrading the cleanliness and feeling of use. The present invention relates to a hair cleansing agent that can impart a good feel to natural hair, such as a feeling of slipping, moist feeling, and the natural suppleness of healthy hair.

Hair is always exposed to physical stimuli due to daily hair care behavior such as dryer heat, brushing, and chemical stimuli such as shampoo, perm, hair color, bleach, etc. It is in a damaged state. In addition, the change in hair quality with aging accelerates these damages and causes the loss of the suppleness of healthy hair.

In general, protection / restoration of damaged hair is performed in a form that compensates for components, structures, and their analogs that have been lost due to damage. The interaction (affinity) between the protective base and hair is considered to be important for the expression of the protective / repair function, and at present, the method using sphingolipids and protein derivatives as the protective base is widely used as a useful technique. . For example, there is a hair cleansing agent containing a surfactant composed of an anionic surfactant and a zwitterionic surfactant, a cationic polymer, and ceramide or glycoceramide (see Patent Document 1). However, protective bases such as ceramide and glycoceramide have a high melting point and are easily crystallized, so that a sufficient amount cannot be blended. In addition, even a small amount of the formulated protective base does not easily penetrate into the hair, and there is no method that can supply a sufficient amount to the hair. Therefore, the conventional hair cleaning agent has a problem that the function of the formulated protective base cannot be sufficiently exhibited.

  In recent years, amphiphiles have been used in various fields.

  An amphiphilic substance is a substance having two different properties of hydrophilicity and lipophilicity, and a substance having such a property is called a surfactant. For example, a glycolipid has two properties of hydrophilicity derived from the property of sugar and lipophilicity derived from the property of lipid, and is an example of a surfactant.

  Synthetic surfactants have been developed as a result of the development of the petrochemical industry, and the production of synthetic surfactants has increased dramatically compared to surfactants derived from biological components, and now they are indispensable for daily life. It was.

  However, since synthetic surfactants have low biodegradability, environmental pollution is becoming a serious problem as the amount of synthetic surfactants used increases. Therefore, in order to reduce the burden on the environment, biosurfactants with high safety and high biodegradability have been reviewed again, and development of various types of biosurfactants has been desired.

  Examples of the biosurfactant include five types of biosurfactants of glycolipid type, acyl peptide type, phospholipid type, fatty acid type and polymer compound type.

  Among the biosurfactants, lecithin, which is a phospholipid biosurfactant, has been used as an emulsifier for a long time. When suspended in water, the phospholipid associates to form a double membrane. It is known to form vesicles with confined phases. These vesicles are also called liposomes, and are used as models of biological membranes or as carriers for cosmetics and drugs. In addition, the present condition is that little is known about what forms a vesicle in biosurfactants other than a phospholipid type.

  On the other hand, many types of biosurfactants produced by bacteria or yeast have been reported as glycolipid biosurfactants. Glycolipid biosurfactants are not only highly biodegradable, have low toxicity and are environmentally friendly, but also have excellent physiological functions. For example, glycolipid-based biosurfactants are known to have a high moisturizing effect and are expected to be used as ingredients in cosmetics and the like.

One typical glycolipid biosurfactant is mannosylerythritol lipid (hereinafter sometimes referred to as “MEL”). MEL is a substance discovered from Ustilago nuda and Shizonella melanogramma (see Non-Patent Documents 1 and 2). Subsequently, itaconic acid-producing mutant strains of the genus Candida (see Patent Document 2 and Non-Patent Document 3), Candida antarctica (currently Pseudozyma antarctica) (see Non-Patent Documents 4 and 5) It is also reported that it can be produced by yeasts of the genus Kurtzmanomyces (see Non-Patent Document 6). At present, production of 100 g / L or more is possible by continuous culture and production for a long time.

  MEL has 4-O-β-D-mannopyranosyl-meso-erythritol structure and 1-O-β-D-mannopyranosyl-form as shown in the following general formula (1) as optical isomers of erythritol having a sugar skeleton. A meso-erythritol structure (the following general formula (2)) exists.

  By synthesizing one kind of MEL having this 1-O-β-D-mannopyranosyl-meso-erythritol structure, it is proved that the sugar skeleton of the conventional MEL has the structure of the above general formula (1). (Non-Patent Document 7). Most recently, the MEL having a conventional 4-O-β-D-mannopyranosyl-meso-erythritol structure is an optical isomer of 1-O-β-D-mannopyranosyl-meso of the above general formula (2). -It has been found that mass production can be achieved by producing MEL having an erythritol structure using microorganisms such as Pseudozyma tsukubaensis (Patent Document 3).

MEL having a conventional 4-O-β-D-mannopyranosyl-meso-erythritol structure has been reported to have various physiological activities including antibacterial properties, antitumor properties, glycoprotein binding ability (non- Patent Document 8). In addition, this conventional MEL exhibits extremely unique self-assembly characteristics, and not only a slight difference in molecular structure has a great influence on the formation of self-assemblies, but also dilute solutions (6.3 × 10 ^-2 wt% or less) (non-patent document 9). Furthermore, a liquid crystal emulsification technique (Patent Document 4) using a conventional MEL bicontinuous sponge structure is also reported.

JP-A-08-59443 Japanese Patent Publication No.57-145896 WO / 2008/018448 JP 2007-181789 A

R. H. R. H. Haskins, Jay. A. Tone (J. A. Thorn), B. Boothroyd, “Can. J. Microbiol.”, Volume 1, p 749-756 (1955). Gee. G. Deml, Tee. T. Anke, F. F. Oberwinkler, B. M. G. Giannetti, W. Steglich, “Phytochemistry”, Vol. 19, p83-87 (1980). Tee. Nakahara, H. Kawasaki, T. T. Sugisawa, Wy. Y. Takamori, Tee. T. Tabuchi, “Journal of Fermentation Technology” (Japan), Japan Fermentation Engineering Society, 61, p19-23 (1983). Di. Kitamoto, S. Akiba, See. C. Hioki, Tee. T. Tabuchi, “Agric. Biol. Chem.”, (Japan), Japan Society for Agricultural Chemistry, Volume 54. p31-36 (1990). H. S. Kim (H.-S. Kim), Bee. Di. Yoon (B.-D. Yoon), Di. H. D.-H. Choung, H. M. Oh (H.-M. Oh), Tee. T. Katsuragi, Wye. Y. Tani "Appl. Microbiol. Biotechnol.", (Germany), Springer-Verlag, 52, p713-721 (1999). K. kakukawa, M. Tamai, K. Imamura, K. Miyamoto, S. Miyoshi, Y. Morinaga, O. Suzuki, Miyagawa (T. Miyakawa) “Bioscience, Biotechnol. Biochem.” (Japan), Japan Society for Agricultural Chemistry, 66, p188-191 (2002). Di. D. Crich, M. A. Mora, Earl. R. Cruz “Tetrahedron” (Netherlands), Elsevier, 58, p35-44 (2002). Dai Kitamoto “Oreoscience” (Japan), Japan Oil Chemists' Society, Volume 3, p663-672 (2003). Tee. T. Imura, N. N. Ohta, K. Inoue, N. N. Yagi, H. H. Negishi, H. H. Yanagishita, Di. Kitamoto “Chemistry European Journal” (USA), Wiley, 12, pp. 2434-2440 (2006)

In the present invention, the formulated protective base can sufficiently penetrate into the hair, and the biosurfactant itself protects damaged hair, so that the hair after washing has a natural slipperiness, moist feeling, health An object of the present invention is to provide a hair cleansing agent that imparts a good feel such as the suppleness of natural hair and is excellent in preventing and repairing hair damage.

  Further, as described above, biosurfactants are known to have various physiological activities, but biosurfactants having self-accumulation ability have not been well known.

  This invention is made | formed in view of the said conventional problem, Comprising: The objective is to provide the hair washing agent using MEL which has self-accumulation ability.

As a result of intensive studies, the present inventors have found that the above problems can be solved by the following means, and have reached the present invention. That is, the present invention allows the biosurfactant to be regularly arranged on the surface of damaged hair by allowing an anionic surfactant and an organic acid or inorganic acid or a salt thereof to coexist with a biosurfactant having self-accumulation ability. It has been found that a hair cleanser capable of exhibiting a high hair treatment effect by adsorption can be produced, and the present invention has been completed.

That is, the present invention has the following configuration.

1. A hair cleanser containing the following components (A) to (C).
(A) Biosurfactant
(B) Anionic surfactant
(C) Organic acid or inorganic acid or a salt thereof 2. The hair cleansing agent according to 1, wherein the biosurfactant has a mannose skeleton.
3. 3. The hair cleansing agent according to 1 or 2, wherein the biosurfactant has a sugar alcohol linked to a glycoside at the hydroxyl group at the 1-position of the mannose skeleton.
4). The biosurfactant having a mannose skeleton was selected from the group consisting of mannosyl erythritol lipid (MEL), mannosyl mannitol lipid (MML), mannosyl sorbitol lipid (MSL), mannosyl arabitol lipid (MAraL) and mannosyl ribitol lipid (MRL). 3. The hair cleansing agent according to 3, which is one or more compounds.
5). MEL is mannosyl erythritol lipid A (MEL-A), mannosyl erythritol lipid B (MEL-B), mannosyl erythritol lipid C (MEL-C), mannosyl erythritol lipid D (MEL-D), triacyl derivative of MEL-A, 5. The hair cleansing agent according to 4, which is one or more compounds selected from the group consisting of MEL-B triacyl, MEL-C triacyl and MEL-D triacyl.
6). The hair cleansing agent according to any one of 1 to 5, wherein the biosurfactant contains a saturated fatty acid and / or an unsaturated fatty acid.

  The hair cleansing agent of the present invention contains a biosurfactant, particularly MEL. Therefore, the hair cleaning agent of the present invention has an effect of exhibiting excellent characteristics. For example, the hair cleaning agent of the present invention improves hair slip, moist feeling and suppleness, and has the effect of preventing and repairing hair damage.

(Biosurfactant)
The component (A) biosurfactant is a generic name for substances having surface-active ability and emulsifying ability produced by living organisms, and not only exhibits excellent surface activity and high biodegradability, but also has various physiological functions. Therefore, there is a possibility of developing behavior / function different from the synthetic surfactant.

  As the biosurfactant of component (A), mannosyl erythritol lipid (MEL), and as mannosyl alditol lipid (MAL) other than MEL, mannosyl mannitol lipid (MML), mannosyl sorbitol lipid (MSL), mannosyl arabitol lipid (MAraL) ), Mannosyl ribitol lipid (MRL), etc. Among them, it is preferable to use a biosurfactant that forms a lamellar structure or / and a vesicle, and MEL is particularly preferable.

(MEL)
The structure of MEL is shown in general formula (3). In general formula (3), substituent R1 is a C4-C24 aliphatic acyl group which may be the same or different. MEL is classified into four types, MEL-A, MEL-B, MEL-C, and MEL-D, based on the presence or absence of acetyl groups at the 4th and 6th positions of mannose.

Specifically, in MEL-A, in the general formula (3), the substituents R2 and R3 are both acetyl groups. In MEL-B, in the general formula (3), the substituent R2 is an acetyl group, and the substituent R3 is hydrogen. In MEL-C, in general formula (3), substituent R2 is hydrogen, and substituent R3 is an acetyl group. In MEL-D, in general formula (3), substituents R2 and R3 are both hydrogen.

The carbon number of the substituent R1 in the above MEL-A to MEL-D is the carbon number of the fatty acid constituting the triglyceride that is an oil or fat to be contained in the MEL production medium, and the degree of utilization of the fatty acid of the MEL-producing bacterium to be used It depends on. In addition, when the triglyceride has an unsaturated fatty acid residue, it is possible to include an unsaturated fatty acid residue as the substituent R1 unless the MEL-producing bacterium assimilates up to the double bond portion of the unsaturated fatty acid. is there. As apparent from the above description, the obtained MEL is usually in the form of a mixture of compounds having different fatty acid residue portions of the substituent R1.

The composition of the present invention contains mannosyl erythritol lipid having the structure represented by the general formula (4) or the general formula (5). In the general formula (4), the substituent R1 is the same or different aliphatic acyl group having 4 to 24 carbon atoms, and the substituent R2 is the same or different hydrogen or acetyl group. The substituent R3 is hydrogen or an aliphatic acyl group having 2 to 24 carbon atoms. In the general formula (5), the substituent R1 is an aliphatic acyl group having 4 to 24 carbon atoms which may be the same or different, and the substituent R2 is a hydrogen or acetyl group which may be the same or different. The substituent R3 is hydrogen or an aliphatic acyl group having 2 to 24 carbon atoms.

The substituent R1 in the general formula (4) and the general formula (5) is an aliphatic acyl group having 4 to 24 carbon atoms which may be the same or different. The number of carbon atoms of the substituent R1 is not particularly limited as long as it is within the above range, but it is more preferably 8 to 14.

The substituent R1 in the general formulas (4) and (5) may be a saturated aliphatic acyl group or an unsaturated aliphatic acyl group, and is not particularly limited. When it has an unsaturated bond, you may have a some double bond, for example. The carbon chain may be linear or branched. In the case of an oxygen atom-containing hydrocarbon group, the number and position of oxygen atoms contained are not particularly limited.

  The structure of MAL (mannosylalditol lipid) other than MEL is shown in the general formula (6) (wherein the substituents R2 are hydrogen or acetyl groups which may be the same or different). Mannitol, arabitol, ribitol, and sorbitol are added as sugar alcohols (alditol) other than erythritol (n = 4: mannitol, sorbitol, n = 2: arabitol, ribitol). According to the general formula (6), MAL is a saturated or unsaturated straight chain having 2 to 20 carbon atoms, preferably 4 to 18 carbon atoms, more preferably 6 to 14 carbon atoms at the 2nd and 3rd positions of mannose. Having an alkanoyl group having a chain or a branch (wherein the substituent R 2 is hydrogen or acetyl group which may be the same or different)

(In the formula, the substituents R1 may be the same or different and each has a saturated or unsaturated straight or branched chain having 2 to 20 carbon atoms, preferably 4 to 18 carbon atoms, more preferably 6 to 14 carbon atoms. (In the formula, the substituent R2 is a hydrogen atom or an acetyl group, which may be the same or different, and is preferably a compound in which both of the substituents R2 are acetyl groups.)

(Triacyl derivative)
The biosurfactant used in the present invention may be MEL triacyl and MAL triacyl other than MEL. The triacyl biosurfactant is a biosurfactant having higher hydrophobicity than MEL or MAL other than MEL. For example, it also exists in the culture solution of MEL-producing bacteria, and when it is obtained in a large amount, it can be produced by reacting MEL with various vegetable oils using enzymes.

  The triacyl form of MEL, that is, triacyl mannosyl erythritol lipid (sometimes referred to as triacyl MEL), is that if the substituents R1 and R3 are both aliphatic acyl groups in the general formula (4) or the general formula (5), Triacyl MEL, and the substituent R2 is hydrogen or acetyl group which may be the same or different. Similarly to MEL, triacyl MEL is classified into four types of triacyl MEL-A, triacyl MEL-B, triacyl MEL-C, and triacyl MEL-D based on the presence or absence of acetyl groups at the 4th and 6th positions of mannose.

  Triacyl MEL exhibits different properties from diacyl MEL. Specifically, since it has high hydrophobicity, it is excellent as an emollient agent in that it can be easily adapted to various oil components as compared with conventional MEL.

  The biosurfactant preferably used in the present invention is MEL-B having a structure represented by the general formula (7) or the general formula (8).

(In the general formula (7) and the general formula (8), the substituent R1 is the same or different aliphatic acyl group having 4 to 24 carbon atoms)

  In addition, although a biosurfactant may be used independently, 2 or more types of biosurfactants can also be used together.

(Biosurfactant production method)
The method for producing the biosurfactant is not particularly limited, and a fermentation method using microorganisms may be arbitrarily selected. For example, culture production of MEL (MEL-A, MEL-B, MEL-C) can be produced by Pseudozyma antarctica (NBRC 1073) according to a conventional method, and Pseudozyma antarctica, Pseudozyma sp. Can do. It is a well-known fact that an MEL mixture can be easily obtained with any microorganism. The MEL mixture can be purified using silica gel column chromatography to isolate MEL-A, MEL-B and MEL-C. Moreover, as bacteria which produce MEL-B, Pseudozyma antarctica and Pseudozyma tsukubaensis are known, and these bacteria may be used. Pseudozyma hubeiensis, Pseudozyma graminicola, etc. are known as bacteria that produce MEL-C, and these bacteria may be used. The microorganism having the ability to produce MEL is not particularly limited, and can be appropriately used depending on the purpose.

  Fermentation medium for producing biosurfactant includes N sources such as yeast extract and peptone, C sources such as glucose, glycerol and fructose, and inorganic salts such as sodium nitrate, dipotassium hydrogen phosphate and magnesium sulfate heptahydrate. A medium having a general composition can be used, and oil and fats such as olive oil, soybean oil, sunflower oil, corn oil, canola oil and coconut oil, and water-insoluble such as hydrocarbons such as liquid paraffin and tetradecane can be used. Sexual substrates can be used alone or added with two or more.

  Fermentation conditions such as pH and temperature, culture time, and the like can be arbitrarily set, and the culture solution after fermentation can be used as it is as the biosurfactant of the present invention. In addition, it is possible to appropriately add any operation such as filtration, centrifugation, extraction, purification, sterilization and the like to the culture solution after fermentation, and the obtained extract can be diluted, concentrated and dried. .

  As oils and fats used as raw materials, vegetable oils and fats are preferable. Vegetable fats and oils are not particularly limited, and can be appropriately selected according to the purpose. For example, soybean oil, rapeseed oil, corn oil, peanut oil, cottonseed oil, safflower oil, sesame oil, olive oil, palm oil, etc. Among them, soybean oil and olive oil are biosurfactants (especially MEL) production efficiency (production amount) , Production rate, and yield) are particularly preferable. These may be used alone or in combination of two or more.

  There is no restriction | limiting in particular as an inorganic nitrogen source, Although it can select suitably according to the objective, For example, ammonium nitrate, urea, sodium nitrate, ammonium chloride, ammonium sulfate, etc. are mentioned.

  There are no particular limitations on the method for recovering and purifying the biosurfactant, and it can be appropriately selected according to the purpose. For example, it can be recovered by centrifuging the culture solution to recover the oil, and extracting and concentrating with an organic solvent such as ethyl acetate.

  Examples of the extraction solvent include water, alcohols (for example, lower alcohols such as methanol, absolute ethanol, and ethanol, or polyhydric alcohols such as propylene glycol and 1,3-butylene glycol), ketones such as acetone, diethyl ether, and dioxane. , Esters such as acetonitrile and ethyl acetate, and organic solvents such as xylene, benzene, and chloroform can be used alone or in any combination of two or more kinds, and each solvent extract is combined. Can also be used.

  There are no particular limitations on the extraction method, but usually it may be in the range of the boiling point of the solvent from room temperature to normal pressure. After extraction, it is filtered or adsorbed, decolored and purified using an ion exchange resin to form a solution. , Paste, gel, and powder. In many cases, it can be used as it is, but if necessary, further purification treatment such as deodorization and decolorization may be added as long as the effect is not affected. As a purification treatment means such as deodorization and decolorization, an activated carbon column or the like may be used, and a normal means generally applied depending on the extracted substance may be arbitrarily selected. If necessary, a high-purity biosurfactant can be obtained by purification using a silica gel column.

(Triacyl production method)
A method for obtaining a biosurfactant triacyl form will be described by taking a method for producing a MEL triacyl form as an example, but the biosurfactant triacyl form used in the present invention is not limited to a triacyl MEL.

  For example, in order to obtain a triacyl MEL, a triacyl MEL fraction can be purified from a culture solution produced by fermenting a microorganism as described above. In order to obtain a large amount, MEL is dissolved in an organic solvent, a fatty acid derivative such as vegetable oil is added, and esterification or transesterification is performed in the presence of a hydrolase.

  The fatty acid introduced into the erythritol part of MEL may be a long-chain hydrocarbon monovalent carboxylic acid. Further, it may be a saturated fatty acid or an unsaturated fatty acid. In the case of an unsaturated fatty acid, it may have a plurality of double bonds. The carbon chain may be linear or branched. Furthermore, a fatty acid derivative that is a derivative of a fatty acid may be used in the present invention, or a mixture of a fatty acid and a fatty acid derivative may be used in the present invention. The fatty acid or fatty acid derivative introduced into the erythritol part of MEL is preferably derived from oils, higher fatty acids, and synthetic esters.

  The “oils” may be vegetable oil, animal oil, mineral oil, and hardened oil thereof. Specifically, avocado oil, olive oil, sesame oil, camellia oil, evening primrose oil, turtle oil, macadamian nut oil, corn oil, mink oil, rapeseed oil, egg yolk oil, persic oil, wheat germ oil, sasanqua oil, castor oil , Flaxseed oil, safflower oil, cottonseed oil, eno oil, soybean oil, peanut oil, tea seed oil, kaya oil, rice bran oil, kiri oil, jojoba oil, cacao butter, palm oil, horse oil, palm oil, palm kernel oil Animal and vegetable oils such as beef tallow, sheep fat, pork tallow, lanolin, whale wax, beeswax, carnauba wax, molasses, candelilla wax, squalane, and hardened oils thereof. Examples thereof include mineral oil such as liquid paraffin and petrolatum, and synthetic triglycerin such as glycerin tripalmitate. Preferably avocado oil, olive oil, sesame oil, camellia oil, evening primrose oil, turtle oil, macadamia nut oil, corn oil, mink oil, rapeseed oil, egg yolk oil, persic oil, wheat germ oil, southern oil, castor oil, flaxseed oil , Safflower oil, cottonseed oil, eno oil, soybean oil, peanut oil, tea seed oil, kaya oil, rice bran oil, and more preferably olive oil and soybean oil.

  Examples of the “higher fatty acid” include caproic acid, caprylic acid, capric acid, lauric acid, myristic acid, palmitic acid, oleic acid, linoleic acid, linolenic acid, stearic acid, behenic acid, 12-hydroxystearic acid, isostearic acid, Examples include undecic acid, tolic acid, eicosapentaenoic acid, docosahexaenoic acid, and the like. Preferred are lauric acid, myristic acid, palmitic acid, oleic acid, linoleic acid, linolenic acid, stearic acid and undecylenic acid, and more preferred are oleic acid, linoleic acid and undecylenic acid.

  Examples of the “synthetic ester” include methyl caproate, methyl caprylate, methyl caprate, methyl laurate, methyl myristate, methyl palmitate, methyl oleate, methyl linoleate, methyl linolenate, methyl stearate, undecine. Methyl acid, ethyl caproate, ethyl caprylate, ethyl caprate, ethyl laurate, ethyl myristate, ethyl palmitate, ethyl oleate, ethyl linoleate, ethyl linolenate, ethyl stearate, ethyl undecinate, vinyl caproate , Vinyl caprylate, vinyl caprate, vinyl laurate, vinyl myristate, vinyl palmitate, vinyl oleate, vinyl linoleate, vinyl linolenate, vinyl stearate, vinyl undecinate, cetyl octanoate , Octyldodecyl myristate, isopropyl myristate, myristyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, Oren'i acid decyl dimethyl octanoate, cetyl lactate, myristyl lactate, and the like. Preferred are methyl laurate, methyl myristate, methyl palmitate, methyl oleate, methyl linoleate, methyl linolenate, methyl stearate, methyl undecylate, more preferably methyl oleate, methyl linoleate, and methyl undecylate. .

  Triacyl MEL can be produced by dissolving MEL in an organic solvent and reacting as described above. The organic solvent is not limited as long as it can solubilize MEL. What is necessary is just to be able to solubilize a part even if not all can be solubilized. The organic solvent may be a mixture of a plurality of organic solvents. Specifically, for example, methanol, ethanol, propanol, butanol, acetone, propanone, butanone, pentan-2-one, 1,2-ethanediol, 2,3-butanediol, dioxane, acetonitrile, 2-methyl-butane -2-ol, tertiary butanol, 2-methylpropanol, 4-hydroxy-2-methylpentanone, tetrahydrofuran, hexane, DMF, DMSO, pyridine, methyl ethyl ketone and the like. Acetone, tetrahydrofuran, tertiary butanol, acetonitrile and dioxane are preferable, and acetone is more preferable.

  Examples of hydrolases include lipases, proteases, and esterases. It is preferable to use at least one selected from these, and a plurality of hydrolases may be used. Lipase and esterase are preferable, and lipase is more preferable.

  Specifically, for example, MEL purified from the culture solution of MEL-producing microorganisms is dissolved in an organic solvent (for example, acetone), and commercially available lipase (for example, Novozyme 435 (manufactured by Novozymes) etc.) and vegetable oils and fats are dissolved therein. Added.

  In the case of this production method, the reaction temperature is 10 to 100 ° C., preferably 20 to 50 ° C., more preferably 25 to 40 ° C., and stirring may be performed for 1 to 7 days. Further, molecular sieves may be added to the reaction solution. By this manufacturing method, MEL added as a material becomes a triacyl body almost quantitatively.

  The purification of triacyl MEL can be performed according to the above-described purification of MEL.

(Method for producing MEL having saturated fatty acid side chain)
MEL having a saturated side chain can be produced in accordance with the above-described method for producing biosurfactant. At that time, as the fats and oils used as a raw material, it is carried out by adding one or more water-insoluble substrates such as olive oil, saturated fatty acids or esters thereof, hydrocarbons such as liquid paraffin and tetradecane.
The MEL having a saturated side chain can be purified according to the above-described MEL purification.

(Manufacturing method of MAL such as MML)
The production of MAL having alditol other than erythritol, such as MML, can be carried out according to the above-described method for producing biosurfactant. In that case, as a raw material, it carries out by adding alditol, such as mannitol, arabitol, ribitol, sorbitol, alone or in combination.
Purification of MAL having an alditol other than erythritol such as MML can be performed according to the above-described purification of MEL.

As the component (A) biosurfactant, one or more of the above-mentioned biosurfactants may be used in combination, and the content thereof improves hair slip, moist feeling of hair, suppleness, hair damage From the viewpoint of preventing and repairing the hair, 0.001 to 20% by weight in the hair cleansing agent of the present invention is preferable, further 0.15 to 5% by weight, and particularly preferably 0.2 to 3% by weight.

Component (B) anionic surfactants include alkyl (or alkenyl) sulfate, polyoxyalkylene alkyl (or alkenyl) ether sulfate, alkane sulfonate, olefin sulfonate, alkyl benzene sulfonate, alkyl (or Alkenyl) sulfosuccinate, dialkyl (or dialkenyl) sulfosuccinate, polyoxyalkylene alkyl (or alkenyl) sulfosuccinate, alkyl (or alkenyl) ether carboxylate, polyoxyalkylene alkyl (or alkenyl) ether carboxylate, Examples thereof include polyoxyalkylene alkyl (or alkenyl) ether phosphate, fatty acid salt, N 2 -acyl glutamate, N 2 -acyl taurate, N 2 -acyl methyl taurine and the like. Of these, polyoxyethylene alkyl ether sulfates, polyoxyethylene alkenyl ether sulfates, and alkyl sulfates are preferable, and those represented by the general formula (B 1) or (B 2) are particularly preferable.

R ¹⁴ O (CH ₂ CH ₂ O) _a SO ₃ M (B1)
R ¹⁵ OSO ₃ M (B2)

[Wherein R ¹⁴ represents an alkyl group or alkenyl group having 10 to 18 carbon atoms, R ¹⁵ represents an alkyl group having 10 to 18 carbon atoms, and M represents an alkali metal, alkaline earth metal, ammonium, alkanolamine or A basic amino acid is shown, a shows the number of 1-5 on a weight average. ]

Two or more of these components (B) may be used in combination, and the content thereof is 1 to 50% by weight in the hair cleansing agent of the present invention in terms of foaming, liquid properties during use and detergency. More preferably, it is 8 to 30% by weight, particularly 10 to 22% by weight.

Examples of the organic acid of component (C) include carboxylic acids such as monocarboxylic acid, dicarboxylic acid, hydroxycarboxylic acid, and polycarboxylic acid, alkyl sulfuric acid, alkyl phosphoric acid, and the like. Among these, carboxylic acid, particularly dicarboxylic acid, hydroxycarboxylic acid, and the like. Carboxylic acid is preferred. Examples of the dicarboxylic acid include malonic acid, succinic acid, glutaric acid, adipic acid, maleic acid, fumaric acid, and phthalic acid. Examples of the hydroxycarboxylic acid include glycolic acid, lactic acid, hydroxyacrylic acid, oxybutyric acid, and glyceric acid. , Malic acid, tartaric acid, citric acid and the like. Examples of inorganic acids include phosphoric acid, sulfuric acid, and nitric acid. As the component (C), organic acids are preferable, and α-hydroxycarboxylic acids, particularly lactic acid and malic acid are particularly preferable.

Two or more of these organic acids or inorganic acids or their salts of component (C) may be used in combination, and the content thereof allows penetration of component (A) (amphiphilic amide lipid) into the hair. From the viewpoint of promoting, the acid conversion amount is preferably 0.05 to 10% by weight, more preferably 0.1 to 5% by weight, and particularly preferably 0.5 to 2% by weight in the hair cleansing agent of the present invention.

In order to further improve the foam performance, the hair cleansing agent of the present invention may contain a surfactant other than component (B), preferably a nonionic surfactant or an amphoteric surfactant.

As surfactants other than the component (B), that is, amphoteric surfactants or nonionic surfactants, cationic surfactants (alkylamine salts, alkyl quaternary ammonium salts, etc.), amphoteric surfactants: carboxylic acid type amphoteric Surfactant (amino type, betaine type), sulfate ester type amphoteric surfactant, sulfonic acid type amphoteric surfactant, phosphate ester type amphoteric surfactant, nonionic surfactant (ether type nonionic surfactant, Ether ester type nonionic surfactant, ester type nonionic surfactant, block polymer type nonionic surfactant, nitrogen-containing type nonionic surfactant), other surfactants (natural surfactant, protein hydrolysis) Derivatives of polymers, polymeric surfactants, surfactants containing titanium and silicon, fluorocarbon surfactants) and the like.

These surfactants may be used in combination of two or more, and the content is not particularly specified, but is usually 0.0001 to 50% by weight of the total composition, preferably 0.1 to 20% by weight, and higher effects. In obtaining, 0.5 to 15% by weight, particularly 1 to 10% by weight is preferred.

The pH of the hair cleansing agent of the present invention is not particularly limited, and can be used without any problem as long as it is in the range of pH 1 to pH 7. Specifically, the pH at 25 ° C. when diluted 20 times by weight with water is preferably in the range of pH 3 to pH 7, further pH 3 to pH 6, particularly pH 3 to pH 5. For adjusting the pH, each of the acid components or other acids can be used, and inorganic or organic basic substances such as sodium hydroxide and organic amine are used.

The hair cleansing agent of the present invention can contain a silicone derivative or a cationic polymer that is usually used as a feel improving component for the purpose of further improving the feeling of use.

Examples of silicone derivatives include dimethylpolysiloxane, methylphenylpolysiloxane, amino-modified silicone, polyether-modified silicone, epoxy-modified silicone, fluorine-modified silicone, cyclic silicone, alkyl-modified silicone, and oxazoline-modified silicone. Siloxane, methylphenylpolysiloxane, amino-modified silicone, polyether-modified silicone, oxazoline-modified silicone, and cyclic silicone are preferred. Two or more kinds of silicone derivatives may be used in combination, and the content thereof is from 0.01 to 20% by weight, more preferably from 0.05 to 10% by weight, particularly from 0.1 to 5% in the total composition. % By weight is preferred.

Cationic polymers include polydimethyldiallylammonium chloride, acrylamidopropyltrimethylammonium chloride / acrylate copolymer, acrylamide / dimethyldiallylammonium chloride copolymer, methylvinylimidazolinium chloride / vinylpyrrolidone copolymer, hydroxyethylcellulose / diallyldimethylammonium chloride copolymer, vinyl Diethyl sulfate of pyrrolidone / dimethylaminoethyl methacrylate copolymer, vinyl pyrrolidone / dimethylaminoethyl methylate copolymer, vinyl pyrrolidone / alkylamino acrylate / vinyl caprolactam copolymer, vinyl pyrrolidone / dimethylaminopropyl methacrylamide copolymer, O 2-[2- Hydro - 3 - (trimethylammonio) propyl] hydroxyethyl cellulose, guar hydroxypropyltrimonium chloride, and the like. Two or more kinds of cationic polymers may be used in combination, and the content thereof is from 0.1 to 20% by weight, more preferably from 0.05 to 10% by weight, and particularly preferably from 0.5 to 10% by weight in the total composition as a solid content. 1 to 5% by weight is preferred.

The hair cleansing agent of the present invention can contain proteins that are usually used as a hair protecting component for the purpose of improving the moist feeling and suppleness of the hair and further improving the effect of preventing and repairing hair damage.

Proteins include any of proteins, protein hydrolysates and derivatives thereof, and can be obtained by extraction or induction from animals or plants. Examples of animal-derived proteins include keratin, elastin, collagen, lactoferrin, casein, α (β) -lactalbumin, globulins, ovalbumin and hydrolysates thereof, among which keratin, elastin, collagen, casein and These hydrolysates are preferred. Examples of plant-derived proteins include those extracted from wheat, malt, oats, barley, corn, rice, soybeans, broad beans, silk, lupine seeds, potatoes, apricot seeds, and hydrolysates thereof. Of these, wheat protein, soybean protein, silk protein and hydrolysates thereof are preferred. Two or more kinds of proteins may be used in combination, and the content thereof is preferably 0.01 to 5% by weight, more preferably 0.05 to 4% by weight, and particularly preferably 0.1 to 3% by weight in the total composition.

In the hair cleansing agent of the present invention, within the range not impairing the effects of the present invention, in addition to the above components, oily components such as higher alcohols, lanolin derivatives, fatty acid esters of polyethylene glycol; hydroxypropyl methylcellulose, hydroxycellulose, polyvinyl Water-soluble polymers such as alcohol and polyethylene glycol; polyhydric alcohols such as sorbitol; moisturizers; chelating agents such as ethylenediaminetetraacetic acid (EDTA); drugs such as vitamins; amino acids and derivatives thereof; polyethylene, polystyrene, polymethyl methacrylate, Fine polymer powders such as nylon and silicone and their hydrophobized products; extracts extracted from animals and plants; UV absorbers; pearlizing agents; antiseptics; bactericides; anti-inflammatory agents; Agents; pigments; fragrances, etc. depending on the purpose It is possible to focus.

More specifically, within the range that does not impair the effects of the present invention, the components and additives exemplified below (again, including duplicated components and additives) are arbitrarily selected and used in combination. The content in the preparation is not particularly specified, but is usually preferably 0.0001 to 50%.

(1) Various fats and oils Avocado oil, almond oil, fennel oil, sesame oil, olive oil, orange oil, orange raffer oil, sesame oil, cocoa butter, chamomile oil, carrot oil, cucumber oil, beef tallow fatty acid, cucumber nut oil, safflower oil , Shea fat, liquid shea fat, soybean oil, camellia oil, corn oil, rapeseed oil, persic oil, castor oil, cottonseed oil, peanut oil, turtle oil, mink oil, egg yolk oil, palm oil, palm kernel oil, owl, palm Oil, beef tallow, pork tallow, squalene, squalane, pristane or hydrogenated products of these oils (hardened oil, etc.).

(2) Wax beeswax, carnauba wax, whale wax, lanolin, liquid lanolin, reduced lanolin, hard lanolin, candelilla wax, montan wax, shellac wax, rice wax and the like.

(3) Mineral oil liquid paraffin, petrolatum, paraffin, ozokelide, ceresin, microcrystalline wax and the like.

(4) Fatty acids Lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, oleic acid, linoleic acid, linolenic acid, docosahexaenoic acid, eicosapentaenoic acid, 12-hydroxystearic acid, undecylenic acid, tall oil, lanolin fatty acid Synthetic fatty acids such as natural fatty acids such as isononanoic acid, caproic acid, 2-ethylbutanoic acid, isopentanoic acid, 2-methylpentanoic acid, 2-ethylhexanoic acid and isopentanoic acid.

(5) Alcohols: natural alcohols such as ethanol, isopyropanol, lauryl alcohol, cetanol, stearyl alcohol, oleyl alcohol, lanolin alcohol, cholesterol, phytosterol, phenoxyethanol, synthetic alcohols such as 2-hexyldecanol, isostearyl alcohol, 2-octyldodecanol .

(6) Polyhydric alcohols Ethylene oxide, ethylene glycol, diethylene glycol, triethylene glycol, ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, polyethylene glycol, propylene oxide, propylene glycol, polypropylene glycol 1,3-butylene glycol, pentyl glycol, glycerin, pentaerythritol, threitol, arabitol, xylitol, ribitol, galactitol, sorbitol, mannitol, lactitol, maltitol and the like.

(7) Esters isopropyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, myristyl myristate, oleyl oleate, decyl oleate, octyldodecyl myristate, hexyl decyl dimethyloctanoate, cetyl lactate, myristyl lactate, Diethyl phthalate, dibutyl phthalate, lanolin acetate, ethylene glycol monostearate, propylene glycol monostearate, propylene glycol dioleate, and the like.

(8) Metal soaps Aluminum stearate, magnesium stearate, zinc stearate, calcium stearate, zinc palmitate, magnesium myristate, zinc laurate, zinc undecylenate and the like.

(9) gum, saccharide or water-soluble polymer compound gum arabic, benzoin gum, danmar gum, guaiac gum, Irish moss, Karaya gum, tragacanth gum, carob gum, quinseed, agar, casein, lactose, fructose, sucrose or ester thereof, Trehalose or derivatives thereof, dextrin, gelatin, pectin, starch, carrageenan, carboxymethyl chitin or chitosan, hydroxyalkyl (C2-C4) chitin or chitosan to which alkylene (C2-C4) oxide such as ethylene oxide is added, low molecular chitin Or chitosan, chitosan salt, sulfated chitin or chitosan, phosphorylated chitin or chitosan, alginic acid or salt thereof, hyaluronic acid or salt thereof, chondroitin sulfate or salt thereof, heparin, ethylcellulose, methylcellulose, Polycarboxylate such as carboxymethyl cellulose, carboxyethyl cellulose, sodium carboxyethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, nitrocellulose, crystalline cellulose, polyvinyl alcohol, polyvinyl methyl ether, polyvinyl pyrrolidone, polyvinyl methacrylate, polyacrylate, polyethylene oxide and polypropylene oxide Alkylene oxide or its crosslinked polymer, carboxyvinyl polymer, polyethyleneimine and the like.

(10) Surfactant Cationic surfactant (alkylamine salt, alkyl quaternary ammonium salt, etc.), amphoteric surfactant: carboxylic acid type amphoteric surfactant (amino type, betaine type), sulfate ester type amphoteric surfactant , Sulfonic acid type amphoteric surfactant, phosphate ester type amphoteric surfactant, nonionic surfactant (ether type nonionic surfactant, etherester type nonionic surfactant, ester type nonionic surfactant, block Polymer type nonionic surfactants, nitrogen-containing type nonionic surfactants), other surfactants (natural surfactants, derivatives of protein hydrolysates, polymer surfactants, surfactants containing titanium / silicon) , Fluorocarbon surfactants).

(11) Various vitamins Vitamin A group: retinol, retinal (vitamin A1), dehydroretinal (vitamin A2), carotene, lycopene (provitamin A), vitamin B group: thiamine hydrochloride, thiamine sulfate (vitamin B1), Riboflavin (vitamin B2), pyridoxine (vitamin B6), cyanocobalamin (vitamin B12), folic acid, nicotinic acids, pantothenic acids, biotins, choline, inositols, vitamin C group: vitamin C acid or derivatives thereof, vitamin D group: Ergocalciferol (vitamin D2), cholecalciferol (vitamin D3), dihydrotaxosterol, vitamin E group: vitamin E or derivatives thereof, ubiquinones, vitamin K group: phytonadione (vitamin K1), menaquinone (vitamin K2), mena Dione (vitamin K3), menadiol (vitamin K4), other essential fatty acids (vitamin F), carnitine, ferulic acid, γ-oryzanol, orotic acid, vitamin Ps (rutin, eriocitrin, hesperidin), vitamin U and the like.

(12) Various amino acids valine, leucine, isoleucine, threonine, methionine, phenylalanine, tryptophan, lysine, glycine, alanine, asparagine, glutamine, serine, cysteine, cystine, tyrosine, proline, hydroxyproline, aspartic acid, glutamic acid, hydroxylysine , Arginine, ornithine, histidine and the like, and their amino acid derivatives such as sulfate, phosphate, nitrate, citrate or pyrrolidone carboxylic acid.

(13) Various additives derived from plant or animal materials These are processed in a conventional manner according to the type and form of the product to be added (for example, grinding, milling, washing, hydrolysis, fermentation, purification) , Compression, extraction, fractionation, filtration, drying, pulverization, granulation, dissolution, sterilization, pH adjustment, deodorization, decolorization, etc., arbitrarily selected and combined), arbitrarily selected from various materials You can use it.

The solvent used for extraction may be selected in consideration of the intended purpose and type of the product to be provided or the processing to be performed later. Usually, water, methanol, ethanol, propyl alcohol, isopropyl alcohol, butanol 1 type selected from various organic solvents such as lower alcohols such as isobutanol or hydrous lower alcohols, polyhydric alcohols such as propylene glycol, 1,3-butylene glycol and glycerin or hydrous polyhydric alcohols, acetone and ethyl acetate Alternatively, it is desirable to use a mixture of two or more. However, when it is not preferable to contain an organic solvent depending on the application, it is sufficient to use only water or ethanol that can be easily removed after extraction, and it may be used alone or in any mixture with water. The thing extracted and extracted may be sufficient.

In addition, when using additives derived from plant or animal-based ingredients for systemic or topical external preparations and cosmetics, it protects skin and hair, moisturizes, improves touch and texture, imparts flexibility, and stimulates Relaxation, stress relief by fragrance, cell activation (cell aging prevention), suppression of inflammation, improvement of skin and hair quality, prevention of rough skin and its improvement, hair growth, hair growth, hair loss prevention, imparting gloss, cleansing effect, In addition to cosmetic effects such as fatigue relief, blood flow promotion, and warm bath effects, effects such as flavoring, deodorization, thickening, antiseptic, and buffering can also be expected.

In addition, in food and drink, nutritional supplementation, fatigue recovery, tonicity, cell activation (cell aging prevention) and other healthy and cosmetic effects, as well as improved taste, color tone, aroma, gloss, stabilization, thickening, It can be used for antiseptic purposes. In addition to this, various cosmetic and pharmaceutical effects of each of the known raw materials are expected, and by combining these, the intended effect can be promoted or multifunctional. It is also possible to make the product expected to be effective.

Specific plants (herbal medicines) as raw materials include, for example, almonds (toen), ai (indigo leaves), aokazura (Keifu Fuji), Aoki (Aoki), Aogiri or Kenashia Ogiri (Ao Tung), Aotsu Samurai (wooden candy) , Aotenma, acacia, red ginseng, red spruce `` fruit '', red clover, red grapes, red ginseng (logwood), red wrinkles (red buds), red crested or red crested or reddish (root), red crested or reddish (ground yellow), Agi (Aki), Aki larch, Akinire (crusted skin), Aguay Guassou, Aguahe (Omiten coconut palm), Akebi (Kitsu), Asa (Asakojin), Maruba morning glory or morning glory (Chicken), Hydrangea (hydrangea), Ashitaba (Tomorrow), Azuki (Red Azuki), Asunaro, Acerola, Asenyaku (Asenyaku), Athira (Shokuyokanna), Anise, Abiyu, A Biurana, oilseed rape, Abemaki `` fruit '', avocado, ama, achacha (Amacha), Achacharu, Amadokoro (Jade bamboo), Amana (a light-hearted), amaranth (Hyuyu, Hagatetou, Himogeito, Senninkoku, Sugimorigateto, Hosoaogeito, Hasoagoito Aozulan, Amaranthus hypochondriacus), Amigasayuri or Baimo (shellfish mother), Aristolomeria (Yurizuisen), Arcana (Alcanna), Algarrobo (Cabe), Altea, Arnica, Alpinia or Katsumadai (Suzukuku), Aloe (蘆薈), Aloe vera, angelica, apricot or honanzu (Anzujin), Ansokkou (benzoic incense), yellow support, igakozolina (geo-biliary head), licorice or gecko licorice (indian oyster), rush (light heart grass), itadori (tiger cane root), yew (one Place), Strawberry, fig (no flower `` fruit, leaf ''), Ichihatsu (first), Ichibi (winter lion), Ichiyakusou (one medicinal herb), ginkgo (Ginkgo `` seed, leaves ''), Japanese yellow peas (distant), carob, Japanese beetle ( Taillexi), Inubiyu (Honagainubiyu), Rice `` Seeds, Seed coat '', Inondo `` Seeds '', Ibuki-so-sou, Nettle, Ylang-ylang, Iwa-tobacco (Iwabuchi), Iwahiba or Iwamatsu (rolling mushroom), kidney beans, oolong tea, fennel (mustard) , Wheat (Chibusanoki), Ukiyara (Sanryo), Uguisukagura `` Fruit '', Himewikyo, Turmeric (Urban), Duckweed, Usubasaishin or Keirinsaishin or Oshusaishin (spicy), Usvenia Oy, Usbeni Hollyhock, phalaenopsis, weevil (summer hay), udo or shishiudo (gung, alone, tangy), unyadegato (unkari) A, Cat's Claw), Uba tea, Ume (Japanese apricot `` seed, pulp ''), Japanese white beetle, Uwaurushi (Kusako-kemomo), Unshuumika (Chen), Unboku, Estragon, Ezoukogi (Tomogoka), Ezo-suzuran, Echinashi (Hosobamurasaki Barengiku) , Enishida, Enokitake (榎 茸), Ebisugusa or Cassia Tora (Keiko), El Campre, Elderberry “fruit”, Elemi, Elingi or Pre-Lotus Elinji, Enju (Safflower, Camellia rice), Pea, Ogi or Kibanaogi (Yellow Twilight), Naruko Yuri or Kagura-mabanaru Koyuri (Yellow), Ohisasa Yuki or Sasano Yuki, Ouren (Yo Ren), Ogata Houken, Okarasuri (Karokon), Oguruma (Civil Works Incense), Otsu Samurai (Prevention), Psyllium , Car forage), toucan thistle (Zhengzhou leakage, bald eagle), toucan Mi (coconut palm), Toucan cactus, Ovana okera or okera (white moth), Osara squirrel (Banaba), Ooyabaya shabushi "fruit", Oohashigusa (cereal sperm), Omia catechu, Oomihasa or hawthorn (Mountain vegetation), Omiyashi, Omiji Barley), Oka, Okazeri (snake bed), Okra `` fruit '', Oguruma (overturn), Oguri higotai (Zhengzhou leakage, indigo head), Panax ginseng or Tochibaninjin (Ginseng), Hypericum pertussis or Pterotus pertussis or Hypericum perforatum (Brother cut grass), Odorikosou (continuous), Onamomi (Circle ear), Onigomi, Onikoro or Tokoro or Nagadokoro (Hikai), Oninoyara (Tempa), Onyuri or Sasayuri or Hakatayuri (Yuri), Onoelan, Ononis, Halibut楡), Ominae (losing sauce), Yuuko, Dutch mustard (watercress), Ora Dazelli, Dutch beech, Dutch bees, olives `` fruit, seeds, leaves '', oregano, orange `` fruits, peels '', silkworm (ripe yellow), cacao `` fruits, peels, seeds '', oysters (bamboo `` leaves ''), Kagi-Kazura (Katsufuji), Kakidooshi or Kantorisou (Lotus), Kakiran, Gaku Hydrangea, Cassia, Casinoki (fruit berries), Gadju (Kashiwa), Kashiwa (Kashiwagi, Kashiwa), Cascarilla, Cascala Sagrada, gypsophila, gypsophila, cynomolgus (Kinshato), kaniwa, carnation, valerian (valerian), hippopotamus, birch or fern birch (white birch), gerbera, pumpkin, kapokoki `` seed '', kahoku salamander (蜀椒), gama Yellow), chamomile or roman chamomile, chamomile (weed), cam cam (camo duck), carraguwa or yamagwa (mulberry moth), color, caramel salmon, crow Cucumber or cinnamon cucumber (king moth), crow bishak (half-summer), oats, guarana `` seed '', carahoo (shock), karaya, galyu, karin (garden), garcinia, kawamidori, kawara psycho (commissioned vegetables, tranquilized grass) ), Kawajisha, Kawaratake, Kawaranadeshiko (stone bamboo) or Ezokawaranadeshiko (buckwheat, buckwheat), Chinese carrot (blue potato), Chinese mugwort (bamboo chin mackerel), Kansui (sweet potato), licorice (licorice), Kantarasa, Kandelira, Kanto , Canna, kanran, raspberry (Ezo strawberry, Dutch strawberry, shrimp strawberry, nava white strawberry, maple strawberry, European raspberry), kiwi `` fruit, leaf '', kikarasuuri (guro-root), kiku (kikyo, bellflower root), chrysanthemum (chrysanthemum, chrysanthemum) ), Kikutanigiku, Kisage (Kashimi), Gigigishi (Shione), Pheasant (Kashimi), Kizuta Kidachi Aloe, Kidachihakka, Kina, Kinanoki (Cinchona, Red linden), Quinua (Quinoa), Yellowfin (yellow tuna), Kimameme, Gymnema sylvesta, Kimenkaku, Cabbage, Cabbage `` Immature fruit '', Caraway, Cucumber, Gyory (Nishikawa willow) Willow), Kiraja saponaria, Kiraya, Kiransou (golden grass), kumquat `` fruit '', kinkiju, ginsekai, kimbusen, betel, kinzhiki (sentsukakusa), quinlan, ginran, kinryohen, guapeba vermelya, guava `` fruit '', Guayule, Quercus Infectria, cucumber nuts, Kugenumaran, wolfberry (bamboo shoots, coconut leaves, cocoon leaves, ground bark), Japanese cedar (Tenmon Winter), kudu (kudzu), camphor tree, gooseberry `` fruit '', ginseng (Yellow Flower), Kutiti River, Gardenia (mountain梔), Kunugi (樸 樸), Kupuas, Kubeba, Kumazasa, Kumatsura (horse whip grass), Clara (bitter), cranberry `` fruit '', chestnut `` seed, fruit, astringent skin '', curkurigo latifolia `` fruit '', grapefruit `` Fruits / leaves '', buckthorn, black spider (required), kameba toy koshi or kurobana hiki koshi or toki koshi (life-extending grass), cloves (clove, clove), gumbai tuna (sekimei, sekimeishi), kelp Chicken crown flower, chicken crown), gekkavidin, bay bay laurel, Kenashi Sartorii rose (Tsumago, mountain return), gentiana, genus shochu (old lady grass), kemponashi (kigushi), kokisekoku, kushimikan (kouji, tachibana, obi mikan) , Sagami Koji, Ponkan, Santara (Tachibana skin)), Koshinbara (moon flower) Kousei mint, Kozo `` fruit '', Kocha (tea), Kohone (riverbone), Kohon (Tsubakimoto, Karasumoto), Koryang, Koryokkyo (Kora 姜), Cilantro `` fruit '', Kouren (Huo Ren), Koganebana (Yellow Gon) ), Cowberry (Etsu Tachibana), Coco `` fruit '', Goshuyu (Kureo), Gosho strawberry (Golden bonsai), Pepper (Pepper), Cosmos, Copaiba balsam, Coffee `` Seeds, leaves '', Kobushi or magnolia (hot pepper), Burdock (Beef bowl, beef eggplant), coconut lees, sesame (sesame seeds), sesame seeds (genus ginseng), trash beetle (gomisin), honey beetle or binankazura or pine bush, wheat (wheat), rice or rice bran `` red rice cake, white birch '', rice oil, cola・ Acminata `` Seeds '', Cola Vera `` Seeds '', Colonyella, Koroha `` Fruits '', Colombo, Konzlango, Kombu, Konjac, Comfrey (Sardine), Sisal ( Sisal Asa), Sai Hairan, Sakaneran (Ezosa Kanelan), Sasima button eel or cinnabar eel or cinnamon (wei Sensen), Sakura (Oshima cherry, Yamazakura, Oyamazakura, Edhigashi, Bean cherry, Miyazakura, Yoshizakura, Takazakura, Takanezakura, Takanezakura, Takane cherry `` Leaf, flowers, fruits, bark (cherry bark) '', cherries, pomegranates, Sasa, Sababaginran, Sasanka, Sagimodaka (Sawamata), sweet potatoes, sugar cane, sugar beet, saffron jujube (Sanbanjin, Safflower) ), Sapodilla, sapote mariyo, pomelo `` fruit '', savonsou, salmon berry `` fruit '', salamander (sasma), salamander (sand sashimi sashimi), salvia (sage), scallop (mountain vegetables), sawagurumi (mountain walnut) Sangakusa Boten, Sangre de Grado (croton), Sansikuyosou, Sanshichininjin (Ginseng Ginseng), Sanshuyu (Yamamuro), Sansho (Yamamuro), Sandzukon (Yamazune), Shia (Kalite), Cyanoki `` fruit '', Shiitake mushroom, shion (purple spider), winged bean, jikitarisu, shikunshi (messenger), perilla or ajizo or chimmento or catamenzo (purple leaf, shisocho), rosewood, linden tree, syringion, syringae (ream), shimeji , Shakashimeji, Hatake-shimeji, Oshiroi-shimeji, Beech-shimeji, Honjimeshi, Shirotamogitake), Shimoklen (hot pepper), Shimosukeso, potato, Peonies (glaze), Shajin (Sanjin), Jasmine (Matsurika), Janohige (Bulmon) Cowpea, shukukonasumiso, shukushamitsu (sand seed, shredded sand), juzudama, shuro `` Fruit '', Shunran, Zououyashi, Ginger (Ginger), Zouzan Hydrangea (Tsuneyama), Shobu (Koji, Sone), Shozu `` Fruit '', Shirakashi `` Seed '', Silane (Bikkyu), Shirogaucho `` Seed '', White clover ( Clover), white bean (chicken bone grass), white squirrels (scented squirrels), Shirobanatsuta `` flower '', Shirobanarupin, Shirominanten (southern Tenmi), Cinchona sylbula, ginkgo biloba (Ruika, Suika, Shenhua), cinnamon, cinnamum Cassia (Cinnamon), Watermelon (Sairan), Honeysuckle (Gin and Silver Flower, Shinobu), Sorrel (Acid), Swimberry "Fruit", Star Apple, Stevia, Strawberry "Fruit", Psycho (Xulong), Sugina (Question), Surihiyu (horse tooth, horse tooth tender), plum "fruit", sulgaran (oran), red pine "ball"'', Japanese larch, Japanese cypress, Japanese walnut, Japanese hawthorn, Japanese cypress, Japanese dandelion, Japanese horse chestnut (maronier), Japanese pear `` fruit '', Japanese yellow snow, Japanese elder, Japanese juniper, Japanese pine Yarrow (milfoil), rose, pearl butterfly, mistletoe, mint moth or wild mint (Sekoku, Kibanano Sekkok) (Dendrobium, sarcophagus), Cedron (Boushuboku), Zeniaoi, Hirosanega, Senega, Seri, Celery, Senkyu (river cucumber), Sensi Nenren (Penshinren), Sendan, Sennin cactus, Senna `` fruit, leaf '', Senninsou (Otsuka), Senburi (Otsuka), Senboku, Soka (grass), Elephant spinach, Sobain cereals, Somer potato Broad bean, Daio (daihuang), radish, soybeans, Daidai (orange peel, fruit), Takasago Gurihigotai (Southeast indigo sashimi), Takasaburo (Chinese lotus), Takatodai (Daisetsu), Takarabi (Kashiwaji), Dark sweet Cherry `` fruit '', hollyhock, hollyhock (thyme, perilla), tachidokoro (hikai), tachiyanagi, tamarind `` seed '', onion, ram shiba (hot pepper), taranoki `` fruit, leaf, root bark '', tarayo (large leaf winter blue, one leaf (Tea, bitter tea, bitter tea), dahlia (tenjiku button), tarwi, tanuki bean, tanshichiwa, tanjin (dansang), dandelion (Shanghai dandelion) or white dandelion Or mouko dandelion, danmaru, cherry "fruit", chigaya "fruit, root, bud", chikutsu

Carrot (Bamboo ginseng), Chicory, Chunky Piedra (Kidachikomikansou), Tulip, Chokonostay, Datura (Artichoke), Datura (Dahi), Datura (Dark), Datura (Bamboo Leaves), Choreimaitake (猪苓), Chorogi, camellia, tsukushikaneran, tsukuritake (mushroom), tsuchiakebi, camellia, camellia, tsumekusa (lacquered cocoon grass), camellia (duck moth grass), tsuruzuki (red red bean), tsurutsuchiakebi, tsurudukudami (what neck cocoon) Apricot), Tsuru carrot (Yutaba ginseng), Tsurumame, Tsuwabuki, Deiko, Teuchigurumi, Duberry `` Fruit '', Tengusa, Tencha (salmon tea), Tendaiyaku (glaze), Toga (winter coconut), Toka Kizura, Togarashi (banban) , Toki (To-Go), Tokin-Senka (Marigold), Toukai-chika, Tou Navena (river cut), corn or corn hair (southern cocoon), red potato mochi (sadako), turpentine or ginseng (dragon gall), tosa (wood band), dokudami (ten drugs), tokon Mamedaoshi or Echigo quail, eucommia (bark `` bark, leaf, root ''), ash (crustacea), tomato, tragacanth, triacanthus `` seed '', eustoma, tormentilla, dronoki, troroaoi, tongamame, nigerian berry `` fruit '', Chinese yam or Yamanoimo (Yakuyaku), Nagii Kada (Butcher Bloom), Naginata Koju, Nazuna, Rapeseed, Jujube (Sword Bean), Natsumikan, Jujube (Otsugi), Naniwaibara (Golden Cherry), Navena (Continued), Nameko, Nankin Bean (Peanuts), Nanten (Minami Tenmi), Namban Karamushi (Hemp), Nio-Ismille, Nigaki (Nagi), Nigayo Giant (bitter melon), nutmeg, Kay or Nikkei or Ceylon nykki or Yabunikkei (Keishi) or Keishi (Keishi branch), flybird, leek (chicken), elderberry (bone, fruit, flower, stem, leaf), garlic (Otsuka), Nurde (Pyto), Leek, Nemunoki or Nebu or Nebi or Nemurinoki or Jigokubana (Neito), Noazami (Otsugi), Neubara (Yutoshi), Nozenkazura (Ryochohana), Nogateto, Yarrow, Noda Novara, Peach, Pineapple `` Fruit '', Hibiscus (Busouge, Furinbusouge, Roselle), Hilindow, Hauchiwa, Pau Dorse, Hakamuraboshi (bone fracture), Hakkayumatou, Hakusanchidori, Hakusen (white ring skin), Hakulberry `` fruit '' , Hakobe (breeding), hazel (lion), ashridokoro (roth root), basil, lotus (lotus, lotus meat, lotus), parsley (ora) Dandelion), peanuts, batata, wasp or madake (bamboo shoots), patchouli, mint (light load, light load leaf), pepper peas, pearl barley (yokuinin), hanasuge (knowing mother), banana, hana hakka, hanabishi (shitsurishi, shishitsuri), vanilla bins , Papaya, boxweed (pasas), pahalobobo, hub `` whole plant, stem, leaf '', paprika, leafy swan, clam or bee Maikai flower), Hamaboufu (beach windbreak), Hamelis, Palm, Rose (Rose), Balata, Balata rubber tree, Agaric (Agaric, White-faced agaric, Ukimorimorikasa), Haran, Pariurus (Prunus japonicus), Parietalia, Harcanlan, Balsamina ), Harunire (crusted skin, whitened skin, green leaves), Barbasco, Hull Nendo, Handaikai (Saikai Sea), Panoki, Hinogi (Shihi), Hikageturinjin (Party affiliation), Pecan nuts, Higanbana (Ishizuchi, Buddha Shahua), Hygurmadaria, Higotai, Hishi (Rishami), Pistachio, Beet, Hitotsuba (stone wall), Hitotsuenishiida, Daisies (daisies), Hinata Taikozuchi (cow knee), chickpeas, cypress, hiba, castor, sunflower, bell pepper, Himezu (Tempo), Himegai (Kasumi), Himematsutake, Kawariharatake, Hiromatsu ), Gimme orchid, Pimenta `` fruit '', beakshi, beakkyu, jade `` fruit '', chickpea, lentil, belude oyster, broad-leafed oyster (bald eagle), loquat `` fruit, leaf, stem '', betel (large clothes, coconut) , Pu'er tea, Japanese quail (south wicker), Japanese cypress, Japanese dandelion (Japanese winter flower, Japanese winter leaf), Japanese basil (Orchid), Japanese bean ( Beans), Fujimodoki (Chrysanthemum sakura, Camellia), Grapes `` Fruits, pericarps, seeds, leaves '', Beech, Fuyumushinatsutake (Cycium Natsweed), Brazilian licorice, Brazilian carrot, Brazilian palm, Brazilian elephant palm, Black currant `` `` Fruit '', blackberry, branyen, plum `` fruit '', fullceraria, blueberry (Prunus japonica), prunes, floral blanca, blond psyllium, bundo (mung beans), hazelnut, loofah, hetsukaran, safflower (safflower), henken, belladonna, Berries `` fruits '', Persair, Pervianburg, Pereschia grandifolia, crassulaceae or squirrels (scenic sky), Boysenberry `` fruits '', scallops or sardines or nendo or hahagigi kokia (skins), horticultura, scallops ,acute , Persian grass), pouteria sapota, pouteria lucuma, spinach, spinach, physalis (Torne), honoki (Japanese rustic, magnolia), bokeh (wooden moss), hosobaabragiku (painful), hosobaoguruma, hosobanaokera (蒼朮) ), Alaska giraffe (Keiten No.7), Azalea barrengiku, Bodaiju (bamboo tree), buttons (peony `` flowers, leaves, stems, bark ''), button pi (peony skin), hops, jojoba, borage (Lurichisha), Bordeaux , White berry `` fruit '', Hononiku (meat jugo, Daiki), Honsekkok (iron crust stone, Sparrow mountain stone 斛), Mizul tennan show (Tennan star), Maitake (maiko), mao (mao), maca, Macadamia nuts, Margaret (Mokushungiku), Makuri (marine grass), Magwa (mulberry bark `` bark, leaf ''), Magnolia splengeri, Mazaran saba, Masala Suba de Searer, Sheila de Boi, Mashua (Tamanozen Hallen, Kyukon Kinlenka), Matatabi (Kiden Tendon), Matsukasa, pine `` leaves, bark, root '', Matta Orillo, Matsuhodo (茯苓), Matico (Cordon Sillo), Mayoram (Hanahakka), Malvano Jazine (Bitter), Mulberry `` Fruit '', Quince, Malene (Birod mud squid), Mango, Mangosteen, Manzanya (Andesca Mitsule), Manshu gurumi, Mandarin `` Fruit '', Mannentake (Ganoderma), Pheasant (Fruit `` fruit ''), Mishima Saiko (Shibahu), Mizuoobako or Mizuasagao (Dragongrass), Mizokakushi (Half-sided lotus), Misohagi (Chinese cucumber), Michiyanagi or Niwayanagi (Henpotsu), Mitsugashi, Mitsuba, Midori Sapote, Green mint, mimosa, myoga, miracle fruit “fruit”, myrrh, milobaran, wheatwaragiku , Mukugeki, Mokunoki, Mukuroji (life-prolonging skin), Munya, Murasaki (purple root), Murasakikibu or Omurasakikibu (purple pearl), Murasaki corn, Murasaki Kinafuji (Kunming chicken blood wisteria), Megakansa Opuntika, Mehajiki ), Meadowgi, Eurasian primrose (Evening Primrose), Melaleuca, Melissa, Merirot, Melon `` Fruit '', Moukomugi, Mosouchiku, Mokkirin, Mogi (Pepper), Mochinoki, Mokko (woody incense), Momijidaio, Peach (leaf, Seeds, flowers, fruits ''), bean sprouts, morero cherry `` fruits '', Morohaya (burlap), Yakawamura potatoes, yakchi (masutachi), cornflower (cornflower), cornflower mint, yacon, yachabushi or himejashabushi or plantain Yashabushi "fruit, pericarp, fruit ears", Yachiyanagi, Japanese radish (octagonal gold plate), Ya Rigi (willow infestation), willow (kawa willow, chili willow, weeping willow, red willow, cat willow, incolyana, kinu willow, kolyanagi, unryu willow, miyagi willow, palm, yama willow, oak willow, yam , Yabugarashi, Yabukouji (purple gold beef), Yabu tobacco (Tsuruga, Tenmeisei), Japanese burdock (Commercial Land), Japanese alder (Yamamuro), Japanese yam (Yamamushi), Japanese mugwort, Eucalyptus, Yukinoshita (Tiger ear grass), Yucca or Flavifolia, Yuzu `` fruit '', Lily, Yoroigusa, Artemisia (Leaf), Ligan (Thunder circle), Lime `` Fruit '', Rye, Lacanca `` Fruit '', Raspberry `` Leaf, fruit '', Latania (Klamelia), Lackey or Shallot ( White), lavender, longan (longan), agave (a Norse agave, Fukurin agave), Ryokucha (green tea), apple `` fruit, seeds, leaves, roots '', gentian, rubus or suabisimus (cool), Ruritama thistle (Vulgaria chintai, Xinjiang indigo sashimi), litchi (toothpick, toothpick) Core), lettuce (Chisha), red currant `` fruit '', red pitaya, lemon `` fruit '', lemongrass, forsythia or syringae (ream), forsythia, wintersweet, wax palm, locon (reed, root), Logan berry “fruit”, rosemary (mannenrou), rosehip (novara), wasabi, cotton wisteria (Moonjang flower), bituminous (ground) and the like.

As seaweeds, seaweed [Green algae: Chlorella vulgaris, Chlorella pyrenoidosa, Chlorella ellipsoidia, Aonori (Usba aonori, Sugiaonori, Hiraaoori, Boaonori, Hosoeda Aonori), Anaaosa (Aosa)] (Macombu, Resilibu, Hosocome, Mitsuishi Kombu), Wakame, Hirome, Aowakame, Giant Kelp (Macrocystis Integrifolia, Neocitus Ruetkeana), Hijiki, Hibamata, Umiuchiwa, Usubaumiuchiwa Umiuchiwa, Akaba Umiuchiwa, Konami Uchiwa, Okinawa Uchiwa, Usuki Uchiwa, Etsumi Uchiwa], seaweed [Red algae: Hijirimen, Maca (Tengusa), Hiraksa, Syria, Oaxa Caterpillar, Yatabegusa, Yuukiri, Shimatengusa, Tosakanori, Togekirinsai, Amakusakirinsai, Giraffe, Juniper ginkgo rhinoceros, Tsunomata, Totsuka (Yahazutsu notamata), Ezotsunotamata, Togetu no Takita , Yaleus banori, Kagius banori, Susius banori, Haius banori, Akamo mizunori] and the like.

In addition, other algae such as green algae (Chlamydomonas: Chlamydomonas, Akayukimo, Dunaliella: Dunaliella, Chlorococcus: Chlorococcus, Kuwanomimo: Kuwanomimo, Volbox: Ohigegawa, Volbox, Palmera, Yotsumeo, Aomido: Chrysanthemum, Aoumidro, Tirguimidro, Hibidoro: Hibidoro, Aosa: Anaaaosa, Amiaosa, Nagaaosa, Kawanori: Kawanori, Friccella, Shiogusa Magama genus: Magamata, Iwagata: Fusaikawa, Surichogata, Heraiwata, Kurokita, Hanemo, Genus: Mill, Chromel, Sakibutimil, Nagamil, Hira Lemon, Casanori genus: Casanori, Jusmo genus: Jujusmo, Tamajusmo, Mizojuzumo, Mika ヅ kimo, Korekaete, Tsutsumimo genus: Kyukougusa, Hitogususa genus: Humanegusa, Hirohatohigususa, Umushihimoe, Sakikimoe Sumiremo: Sumiremo, Hoshimamidro, Fushinashimidoro etc.).

Cyanobacteria (Suizenjinori: Suizinori, Aoko, Nenju: Kawatake, Ishijelly, Hassai, Yuremo, Rasenmo (Spirulina): Spirulina, Trichodesmium (Iaciasio), etc.

Brown alga Sanadagusa: Sanadagusa, Fukurimamiji, Commongusa: Commongusa, Yahazugusa: Ezoyahaz, Yahazugusa, Uraboshiyahaz, Jigamusa: Jigmygusa, Umichiwagusa, Umamisakugakua, Hakusou : Navarimo, Chasomen genus: Motsuki Chasomen, Matsumo genus: Matsumo, Nagamatsumo genus: Nagamatsumo, Okinawa mozuku: Okinawa mozuku, fake Tomozuku: Niseft Mozuku, Futomozuku: Futomozuku, Ishimozuku: Ishimozuku, Kuromo: Kuromo, Nisemozuku: Nisemozuku, Mozuku: Mozuku, Ishige: Ishige, Iroro, Ichimosa: Ichimegasa Umibosu, Urushigusu: Urushigusa, Keurushigusa, Tobaccogusa, Composita: Composita, Haberodomo: Haberodoki, Havanori: Habanori, Habanori: Habanori, Commonbukuro, Ezobukuro, Ezobukuro Chishima fukuronori genus: Chishima fukuronori, Kagomenori genus: Kagomenori, Muraridori genus: Murachidori, shark genus: shark zusa, genus genus: lobster, genus genus: yokojimamanori, Ganoderma genus: cynomolgus, fennel spider: fennel spider, vine genus: turmo, turtle genus: sea turtle, scorpion genus: sujime, misji kumbu genus: misji kumbu, atsuba misji kumbu, kumbu genus: tsutsugara kumbu, oyster kumbu, onikombu, gakonbubu Barracuda, Trollocombu: Trollocombu, Antokume: Antokume, Kajime: Kajime, Tsuruarame, Kurome, Kikuishikombu: Kikuishicombu, Nejikcombu: Nejicombu, Kuroshiome: Kuroshiome, Nekoshikonbu, Aramei Genus: Ainuwakame, Chigaiso, Oniwakame Ezoi Shige: Ezoi Shige, Yabane Moku: Yabane Moku, Rappa Moku: Rappa Moku, Joromoku: Ugano Moku, Jo Lomoku, Hiemoku, Honda Walla: Tamanashimoku, Isomoku, Nagashimamoku, Akamoku, Shidamoku, Honda Walla, Nejimoku, Narasamo, Mametawala, Tatsukuri, Yatsumata Moku, Umitora no Moku, Fushishimoku, Mohmokumoku, Hahamokumoku , Ukimo: Ouokimo, Burukimo: Burukimo, Kayamonori: Kayamonori, etc.).

Red algae Genus: Sea urchins, Tsukumonori, Anemone crickets, Astragalus, Amaranthus, Amaranthus, Agaricata Genus: Tamaitadaki, Kaikekenori Genus: Kaginori, Kaikekenori, Tengusa: Himetengususa, Hytengususa, Oobusa, Nambugu , Kohira, Yohira, Kinukusa, Hibiro, Genus: Hibirodo, Himebiro, Isomumedo: Isommedo, Amphidia: Rhizome, Ryumonso Naminohana: Hosobanaminohana, Naminohana, Sangomodoki: Ragaramomodoki, Shiogusagoromo: Shiogusagoromo, Iwanokawa: Etsukiiwanawa, Kinokawa: Kinokawa, Kanonote, Sangori Kyono Shiro, Sakuranori, Nikumukade, Tambanori, Tsurutsuru, Fudarak, Marubahdarak, Isonohana: Nurakusa, Ronulaksa, Oomkadenori, Hirakintoki: Hirakintoki, Matabou: Matabou, Kintoki: Chabokintoki, Kintoki, Matsunori, Rice, Tokamatsu, Hitomatsu, Fushikintoki, Tsunomude, Nagakinito, Crayfish Nagaobane: Naganobane, Funori genus: Hanafonori, Fukurofunori, Mafunori, Karekigusa: Karekigusa, Tosakamodoki: Hosobanatosakamodoki, Hironotosakamodoki, Yatsudegatatosakamodoki, Kurotosakamodokisane, Zotosofomoza Tosakamodoki, Nankaitosakamodoki, Himetosakamodoki, Kinuhada: Kinuhada, Ezotosaka: Ezotosaka, Tsukasanori: Enashikarimenia, Otsukasanori, Hanaga Tacarimenia, Hoonoo: Honoo, Hikagenoit: Hikagenoit, Usuginu, Nikuhounoo: Nikuhounoo, Venus nago, Venus nago, Susca veni: Suscabeni, Okarugusa: Yamadagusa, Mirin: Mirin, Hosokamiso , Isomocca genus: Isomocca, Yukari genus: Yukari, Hosoyukari, Ibaranori genus: Ibaranori, Saidaibara, Tachiibara, Kagibaranori, Kojinoo: Kizino-o, Isodantsu: Isodantsu, Atsubanori: Atsubanori, Ogonori, Ogonori, Ogori Mizo-okonori, birch, beniogonori, fukureshinori, purple birch, shinkaibanori, spruce birch, kataogonori, ryukyuogono , Scorpionfish, tsutsuginu, yumigataonogori, kubireogonori, mosagogonori, kinubanori, genus genus: beetle genus, genus genus, genus genus , Rhinoceros, spinach, spinach genus: spinach, cedar genus: squid, reeds, hornwort, spiders Genus: Taoyagisou, Hanasakura, Fukurotsunagi Genus: Fukurotsunagi, Sugikonori, Hananoeda Genus: Hananoeda, Hiratakaoyagi: Hiratakaoyagi, Darus : Dulse, Scorpionus, Anadalus, Weibusa: Uebusca, Benifronori: Benifuronori, Fusunagi: Futsutsunagi, Himefusunagi, Hirohafushitsugi, Otsunagisou, Hirawatagigi, Usuwasugi , Egonori: Egonori, Futogigi, Saeda: Saeda, Chirimomiji: Chirimomiji, Konohori family: Habutaenori, Konohori, Suzuronori, Usubeni: Genus: Kagios vanori, Yareus vanori, Susius vanori, Heius vanori, Usbanori modoki: Usbanori modoki, Ayanishiki: Ayanishi Genus: Aeginu, Dadia: Enasidadia, Shimadadia: Isohagi, Shimadadia, Dajimodoki: Dajimodoki, Euglena: Morayogusa, Fuyugusa, Makuri: Makri, Yanaginogi, Yuna, Yukinagina , Sasabayanaginori, Sozo genus: Kurosozo, Kobsozo, Hanesozo, Sozonohana, Hanegusa genus: Hananegusa, Hananegusa, Kozanemo genus: Kozanemo, Isomurasaki, Hosozozanemo, Himegoshigusu, Giant genus Isomagusa, Suginasugusa: Suginasusa, Isobashou: Isobasho, Fujimatsumo: Fujimatsumo, Sawweed: Haeshinosakiba, Kawamoku Kawamozuku, Aokawamozuku, Himekawamozuku, Ideyukogome genera: Ideyukogome, Okichimozuku genera: Okichimozuku, Itogusa genus, Chinorimo genera: Chinorimo, Chisujinori genera: Chisujinori, etc.).

Axial algae (Shajikumo, Shiratamamo, Hoshitsurimo: Hoshitsurimo, Rikunotumusu, Flaskmo: Himebumo, Chaboflasmo, Tripera, etc.), yellow algae (Hikarimo: Hikarimo, etc.), etc.

In addition, the materials derived from animal materials include chicken crown extract, cow or pig, human placenta extract, cow or pig stomach, duodenum, intestine, spleen extract or decomposition product thereof, cow or pig brain tissue Extracts, cattle / pig or fish collagen hydrolyzate (acid, alkali, enzyme etc.) degradation products, water-soluble collagen or collagen derivatives such as acylated collagen, cow or pig elastin or elastin hydrolyzate (acid, alkali, Enzymes etc.) or water-soluble elastin derivatives, keratin and its degradation products or their derivatives, silk proteins and their degradation products or their derivatives, porcine or bovine blood cell protein degradation products (globin peptides), cattle or porcine hemoglobin degradation products (Hemin, hematin, heme, protoheme, heme iron, etc.), milk, casein and its degradation products or their derivatives, skim milk powder and their degradation products or their inducements Body, lactoferrin or decomposed product thereof, egg ingredients, fish hydrolyzate, nucleic acid-related substance (ribonucleic acid, deoxyribonucleic acid), or the like.

In addition, as a material derived from a plant or animal material, any site, cell, tissue, organ, metabolite, etc. derived from a gene recombinant or a cell fusion can be used. Furthermore, for example, an undifferentiated cell group, a cell group in the middle of differentiation, or the like obtained by culturing an arbitrary site, cell, tissue, organ or the like can also be used.

(14) Seawater such as deep seawater, such as sea salt, dried seawater, dead sea or Atlantic or Pacific seawater (sodium chloride, magnesium chloride, potassium chloride, etc.), sea mud or mud ( Fango), for example, Italian mud, German fungo, Eifelfango, Freiburg fungo, etc. Sea mud or mud from various locations (containing components: silicon dioxide, titanium dioxide, aluminum oxide, iron oxide, manganese oxide, sodium oxide, potassium oxide, Magnesium oxide, calcium oxide, strontium oxide, sodium, potassium, magnesium, calcium, chromium, iron, copper, nickel, zinc, lead, manganese, arsenic, water), saintoku stone, black meteorite, etc.

(15) Microbial culture metabolite yeast metabolite, yeast extract, bacterial metabolite, bacterial extract, mold or actinomycete metabolite, mold or actinomycete extract, natto metabolite, natto extract, rice fermentation extract , Fermented extract of rice bran (red rice bran, white rice bran), Euglena extract or decomposition product thereof or water-soluble derivatives thereof, trehalose or derivative thereof, lactic acid fermentation product of raw milk or skim milk powder, lactic acid bacteria fermentation product of legumes, coconut plant Lactic acid bacteria fermented products.

(16) α-hydroxy acids glycolic acid, citric acid, malic acid, tartaric acid, lactic acid and the like.

(17) Inorganic pigments Silicic anhydride, magnesium silicate, talc, kaolin, bentonite, mica, titanium mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, calcium carbonate, magnesium carbonate, yellow iron oxide, Bengala, black iron oxide, gunjo, chromium oxide, chromium hydroxide, carbon black, calamine, etc.

(18) UV absorber / blocker benzophenone derivatives (2-hydroxy-4-methoxybenzophenone, 2-hydroxy-4-methoxybenzophenone-5-sulfonic acid, sodium 2-hydroxy-4-methoxybenzophenone-5-sulfonate, dihydroxy Dimethoxybenzophenone, dihydroxydimethoxybenzophenone-sodium sulfonate, 2,4-dihydroxybenzophenone, tetrahydroxybenzophenone, etc.), paraaminobenzoic acid derivatives (paraaminobenzoic acid, ethyl paraaminobenzoate, glyceryl paraaminobenzoate, amyl paradimethylaminobenzoate, Octyl paradimethylaminobenzoate), methoxycinnamate derivatives (ethyl paramethoxycinnamate, isopropyl paramethoxycinnamate, octyl paramethoxycinnamate, 2-ethoxycinnamic paracinnamic acid) Til, sodium paramethoxycinnamate, potassium paramethoxycinnamate, diparamethoxycinnamate mono-2-ethylhexanoate glyceryl, etc.), salicylic acid derivatives (octyl salicylate, phenyl salicylate, homomenthyl salicylate, dipropylene glycol salicylate, ethylene glycol salicylate, myristyl salicylate, Methyl salicylate, etc.), anthranilic acid derivatives (methyl anthranilate, etc.), urocanic acid derivatives (urocanic acid, ethyl urocanate, etc.), coumarin derivatives, amino acid compounds, benzotriazole derivatives, tetrazole derivatives, imidazoline derivatives, pyrimidine derivatives, dioxane derivatives , Camphor derivative, furan derivative, pyrone derivative, nucleic acid derivative, allantoin derivative, nicotinic acid derivative, vitamin B6 derivative , Umbelliferone, esculin, benzyl cinnamate, cinoxalate, oxybenzone, dioxybenzone, octabenzone, thrisobenzone, benzoresorcinol, arbutin, guaiazulene, shikonin, baicalin, baicalein, berberine, neoheliopan, escalol, zinc oxide, talc, kaolin, etc. .

(19) whitening agent paraaminobenzoic acid derivative, salicylic acid derivative, anthranilic acid derivative, coumarin derivative, amino acid compound, benzotriazole derivative, tetrazole derivative, imidazoline derivative, pyrimidine derivative, dioxane derivative, camphor derivative, furan derivative, pyrone derivative, Nucleic acid derivatives, allantoin derivatives, nicotinic acid derivatives, vitamin C or derivatives thereof (vitamin C phosphate magnesium salt, vitamin C glucoside, etc.), vitamin E or derivatives thereof, kojic acid or derivatives thereof, oxybenzone, benzophenone, arbutin, guaiazulene, Shikonin, baicalin, baicalein, berberine, placenta extract, ellagic acid, lucinol, etc.

(20) Tyrosinase activity inhibitor vitamin C or a derivative thereof (vitamin C phosphate magnesium salt, vitamin C glucoside, etc.), hydroquinone or a derivative thereof (hydroquinone benzyl ether, etc.), kojic acid or a derivative thereof, vitamin E or a derivative thereof, N-acetyltyrosine or derivatives thereof, glutathione, hydrogen peroxide, zinc peroxide, placenta extract, ellagic acid, arbutin, lucinol, silk extract, plant extract (camille, mulberry, gardenia, pearl oyster, clam, clam, mugwort, honeysuckle) , Kihada, dokudami, matsuhod, pearl barley, nettle, hops, hawthorn, eucalyptus, yarrow, altea, keihi, mankaishi, hamamelis, karaguwa or yamagwa, life-prolonging grass, bellflower, toshishi, goblin, gyu, mao, senki (Syu, Dokatsu, Psycho, Bowfu, Hamaboufu, Ogon, Peony skin, Peonies, Gennoshoko, Kuzune, Licorice, Pyramid, Aloe, Shouma, Safflower, Green tea, Black tea, Asen).

(21) Melanin pigment reduction / decomposition substance phenylmercury hexachlorophene, mercuric oxide, mercuric chloride, hydrogen peroxide, zinc peroxide, hydroquinone or its derivative (hydroquinone benzyl ether).

(22) Turnover promoting action / cell activator hydroquinone, lactic acid bacteria extract, placenta extract, ganoderma extract, vitamin A, vitamin E, allantoin, spleen extract, thymus extract, yeast extract, fermented milk extract, plant extract (aloe, Ogon, Japanese horsetail, Gentian, Burdock, Shikon, Carrot, Camelis, Hop, Yokuinin, Odori, Semburi, Japanese cypress, Japanese red snapper, Achacha, Hypericum, Cucumber, Necklace, Mannenrou, Parsley).

(23) Astringents Succinic acid, allantoin, zinc chloride, zinc sulfate, zinc oxide, calamine, zinc paraphenolsulfonate, potassium aluminum sulfate, resorcin, ferric chloride, tannic acid (including catechin compounds) and the like.

(24) Active oxygen scavenger SOD, catalase, glutathione peroxidase and the like.

(25) Antioxidant vitamin C or a salt thereof, stearic acid ester, vitamin E or a derivative thereof, nordihydrogua ceretenoic acid, butylhydroxytoluene (BHT), butylhydroxyanisole (BHA), hydroxytyrosol, parahydroxyanisole , Propyl gallate, sesamol, sesamorin, gossypol, propolis, etc.

(26) Lipid peroxide production inhibitor β-carotene, plant extract (sesame culture cells, hamcha, hypericum, hamamelis, clove, melissa, birch, birch, salvia, mannenro, Nanten, Ajitsu, Ginkgo, green tea).

(27) Anti-inflammatory agent ictamol, indomethacin, kaolin, salicylic acid, sodium salicylate, methyl salicylate, acetylsalicylic acid, diphenhydramine hydrochloride, d-camphor, dl-camphor, hydrocortisone, guaiazulene, camazulene, chlorpheniramine maleate, glycyrrhizic acid or its Salt, glycyrrhetinic acid or a salt thereof, licorice extract, sicon extract, age extract, propolis, etc.

(28) Antibacterial / bactericidal / disinfectant acrinol, sulfur, calcium gluconate, chlorhexidine gluconate, sulfamine, mercurochrome, lactoferrin or hydrolyzate thereof, alkyldiaminoethylglycine chloride solution, triclosan, sodium hypochlorite, chloramine T, Sarashi powder, iodine compound, iodoform, sorbic acid or its salt, propionic acid or its salt, salicylic acid, dehydroacetic acid, parahydroxybenzoic acid esters, undecylenic acid, thiamine lauryl sulfate, thiamine lauryl nitrate, phenol, cresol, p -Chlorophenol, p-chloro-m-xylenol, p-chloro-m-cresol, thymol, phenethyl alcohol, O-phenylphenol, Irgasan CH3565, halocarban, hexachlorophene, chlorohexidine, ester Diol, methanol, isopropyl alcohol, benzyl alcohol, ethylene glycol, propylene glycol, 2-phenoxyethanol, 1,2-pentanediol, zinc pyridione, chlorobutanol, isopropylmethylphenol, nonionic surfactant (polyoxyethylene lauryl ether, Polyoxyethylene nonyl phenyl ether, polyoxyethylene octyl phenyl ether, etc.), amphoteric surfactant, anionic surfactant (sodium lauryl sulfate, lauroyl sarcosine potassium, etc.), cationic surfactant (cetyltrimethylammonium bromide, benzalkco chloride) Nium, benzethonium chloride, methyl rosaniline chloride), formaldehyde, hexamine, brilliant green, malachite green, crystal bio Tsu DOO, Jamaru photosensitive No. 101-containing, photosensitizer No. 201, photosensitizer No. 401, N- long chain acyl basic amino acid derivative and its acid shark salts, zinc oxide, hinokitiol, Sophora root, propolis, and the like.

(29) Moisturizer glycerin, propylene glycol, 1,3-butylene glycol, polyethylene glycol, glycerin tricaprycapric acid, glycolic acid (α-hydroxy acid), hyaluronic acid or salt thereof, chondroitin sulfate or salt thereof, water-soluble chitin or Derivatives or chitosan derivatives, pyrrolidone carboxylic acid or salts thereof, sodium lactate, urea, sorbitol, amino acids or derivatives thereof (valine, leucine, isoleucine, threonine, methionine, phenylalanine, tryptophan, lysine, glycine, alanine, asparagine, glutamine, serine Cysteine, cystine, tyrosine, proline, hydroxyproline, aspartic acid, glutamic acid, hydroxylysine, arginine, ornithine, histidine and their sulfates, Phosphate, nitrate, citrate, or pyrrolidone carboxylic acid), oils and fats “Avocado oil, almond oil, fennel oil, sesame oil, olive oil, orange oil, orange rafa oil, sesame oil, cocoa butter, chamomile oil, carrot oil , Cucumber oil, beef tallow fatty acid, quince seed oil, cucumber seed oil, safflower oil, shea fat, liquid shea fat, soybean oil, camellia oil, corn oil, rapeseed oil, jojoba oil, persic oil, castor oil, sunflower oil, cottonseed oil , Peanut oil, turtle oil, tea tree oil, mink oil, egg yolk oil, palm oil, palm kernel oil, molasses, coconut oil, eucalyptus oil, beef tallow, squalene, squalane, pristane or hydrogenated products of these oils and fats ( Hardened oil) ”, waxes (beeswax, carnauba wax, whale wax, lanolin, liquid lanolin, reduced lath Phosphorus, hard lanolin, candelilla wax, montan wax, shellac wax, rice wax), mineral oil (liquid paraffin, petrolatum, paraffin, ozokelide, ceresin, microcristan wax), natto bacteria metabolites, natto extract, silk fiber extract, plant extract (Akamatsu sap, aloe, kudzu, chamomile, licorice, cucumber, licorice, rice or rice bran, purple root, birch or birch sap, assembly, mulberry white skin, birch leaf, loofah, peony skin, persimmon) and the like.

(30) Elastase activity inhibitor diisopropyl fluorophosphate, plant extracts (Ogon, Hypericum, Clara, Mulberry leaves, Keihi, Genso pepper, Comfrey, Salvia, Elderberry, Bodaiju, Buttonpi), seaweed extract and the like.

(31) Hair selenium disulfide, alkylisoquinolinium bromide solution, zinc pyrithione, biphenamine, thianthol, castari tincture, pepper tincture, pepper tincture, quinine hydrochloride, strong ammonia water, potassium bromate, sodium bromate, thio Glycolic acid and the like.

(32) Antiandrogen follicular hormone (estrone, estradiol, ethinyl estradiol, etc.), isoflavone, oxendron, etc.

(33) Peripheral blood flow promoter Vitamin E or its derivatives, assembly extract, garlic extract, carrot extract, aloe extract, gentian extract, pearl extract, cephalanthin, carpronium chloride, minoxidil and the like.

(34) Stimulants chili tincture, nonyl acid vanillamide, cantalis tincture, ginger tincture, peppermint oil, l-menthol, camphor, benzyl nicotinate and the like.

(35) Metabolic activator Photosensitive element 301, hinokitiol, pantothenic acid or derivatives thereof, allantoin, placenta extract, biotin, pentadecanoic acid glyceride and the like.

(36) Antiseborrheic agent pyridoxine or a derivative thereof, sulfur, vitamin B6 and the like.

(37) The keratolytic agent resorcin, salicylic acid, lactic acid, urea and the like.

(38) Oxidizing agent hydrogen peroxide solution, sodium persulfate, ammonium persulfate, sodium perborate, urea peroxide, sodium percarbonate, sodium tripolyphosphate, sodium bromate, potassium bromate, sodium pyrophosphate, Sodium orthophosphate, sodium silicate hydrogen peroxide adduct, sodium sulfate hydrogen peroxide adduct, sodium chloride hydrogen peroxide adduct, β-tyrosinase enzyme solution, mushroom extract, etc.

(39) Hair removal agents Inorganic reducing agents such as strontium sulfate, sodium sulfide, barium sulfide, calcium sulfide, thioglycolic acid or its salts (calcium thioglycolate, sodium thioglycolate, lithium thioglycolate, magnesium thioglycolate) Strontium thioglycolate) and the like.

(40) Hair swelling agents such as ethanolamine, urea and guanidine.

(41) Dye agent
5-aminoorthocresol, 2-amino-4-nitrophenol, 2-amino-5-nitrophenol, 1-amino-4-methylaminoanthraquinone, 3,3'-iminodiphenol, 2,4-diaminophenoxyethanol hydrochloride 2,4-diaminophenol hydrochloride, toluene-2,5-diamine hydrochloride, nitroparaphenylenediamine hydrochloride, paraphenylenediamine hydrochloride, N-phenylparaphenylenediamine hydrochloride, metaphenylenediamine hydrochloride, orthoaminophenol, N-phenyl acetate Paraphenylenediamine, 1,4-diaminoanthraquinone, 2,6-diaminopyridine, 1,5-dihydroxynaphthalene, toluene-2,5-diamine, toluene-3,4-diamine, nitroparaphenylenediamine, paraaminophenol, para Nitroorthophenylenediamine, paraphenylenediamine, paramethylamino Phenol, picramic acid, sodium picramate, N, N'-bis (4-aminophenyl) -2,5-diamino-1,4-quinonediimine, 5- (2-hydroxyethylamino) -2-methylphenol, N -Phenylparaphenylenediamine, metaaminophenol, metaphenylenediamine, 5-aminoorthocresol sulfate, 2-amino-5-nitrophenol sulfate, orthoaminophenol sulfate, orthochloroparaphenylenediamine sulfate, 4,4'-diamino sulfate Diphenylamine, 2,4-diaminophenol sulfate, toluene-2,5-diamine sulfate, nitroparaphenylenediamine sulfate, paraaminophenol sulfate, paranitroorthophenylenediamine sulfate, paranitrometaphenylenediamine sulfate, paraphenylenediamine sulfate, parasulfate sulfate Methylaminophenol, metaaminosulfate Diol, metaphenylenediamine sulfate, catechol, diphenylamine, α-naphthol, hydroquinone, pyrogallol, phloroglucin, gallic acid, resorcin, tannic acid, 2-hydroxy-5-nitro-2 ', 4'-diaminoazobenzene-5'-sulfone Sodium acid, hematein, etc.

(42) Fragrance Natural animal fragrances such as musk, civet, castrium, ambergris, anise essential oil, angelica essential oil, ylang ylang essential oil, iris essential oil, fennel essential oil, orange essential oil, cananga essential oil, caraway essential oil, cardamom essential oil, guayakwood essential oil, Cumin essential oil, black letter essential oil, cinnamon essential oil, cinnamon essential oil, geranium essential oil, copaiba balsam essential oil, coriandel essential oil, perilla essential oil, cedarwood essential oil, citronella essential oil, jasmine essential oil, gingergrass essential oil, cedar essential oil, spearmint essential oil, western peppermint essential oil, Otsuka perfume essential oil, tuberose essential oil, clove essential oil, orange flower essential oil, winter green essential oil, trout balsam essential oil, buttery essential oil, rose essential oil, palmarosa essential oil, persimmon essential oil, hiba essential oil, sandalwood essential oil, petit gren essential oil, bay essential oil, vetiva essential oil, bergamot Essential oil , Peru balsam essential oil, boad rose essential oil, melamine essential oil, mandarin essential oil, eucalyptus essential oil, lime essential oil, lavender essential oil, linaloe essential oil, lemongrass essential oil, lemon essential oil, rosemary (mannenrou) essential oil, Japanese mint mint essential oil Other synthetic fragrances.

(43) Dye and Colorant Red Cabbage Dye, Red Rice Dye, Akane Dye, Anato Dye, Ikumi Dye, Turmeric Dye, Enju Dye, Krill Dye, Amber Dye, Caramel, Gold, Silver, Gardenia Dye, Corn Dye, Onion Dye , Tamarind pigment, spirulina pigment, buckwheat whole plant pigment, cherry pigment, laver pigment, hibiscus pigment, grape juice pigment, marigold pigment, purple potato pigment, purple yam pigment, lac pigment, rutin etc.

(44) Other hormones, sequestering agents, pH adjusters, chelating agents, antiseptic and antibacterial agents, cooling agents, stabilizers, emulsifiers, animal and vegetable proteins and their degradation products, animal and vegetable polysaccharides And its degradation products, animal and plant glycoproteins and their degradation products, blood flow promoters, anti-inflammatory agents, anti-inflammatory agents, anti-allergic agents, wound treatment agents, foam increasing agents, thickeners, enzymes, purified water (electronic Water, small clustering, etc.), oral preparations, deodorizing / deodorizing agents, bittering agents, etc. are raised, and when used in combination with these, additive effects or synergistic improvements of the effects of the present invention or other cosmetic effects can be achieved. I can expect.

Moreover, the dosage form of the hair washing agent of this invention is arbitrary, It can mix | blend and use in forms, such as a capsule, powder, granule, solid, liquid, gel, bubble, emulsion, cream, ointment, sheet | seat.

Specifically, for example, cosmetics for external use, cosmetics such as creams, lotions, oils, shampoos, rinses, hair treatments, pre-hair treatments, hair creams, pomades, hair sprays, hair styling agents, permanents, hair nicks, dyeings It can also be used for hair washing and hair cosmetics such as hair, hair growth and nourishing, and other general cosmetics.

In addition, about the method of addition to the hair washing agent of this invention, it may add previously, may add in the middle of manufacture, and should just select it suitably in view of workability | operativity.

  The following examples further illustrate the present invention in detail but are not to be construed to limit the scope thereof.

Example 1 Production of MEL-A and Triacyl MEL-A Seed culture was performed by inoculating 1 loop of Pseudozyma antarctica NBRC 10736 colony into a seed medium (20 ml / 500 ml Sakaguchi flask). Cultured overnight at 30 ° C. The obtained culture broth was used as an inoculum. The composition of the seed medium was 4% Glucose, 0.3% NaNO ₃ , 0.02% MgSO ₄ .7H ₂ O, 0.02% KH ₂ PO ₄ , and 0.1% yeast extract. For the culture, 75 ml of the above inoculum was inoculated into 1.5 L (5 L-jar) of production medium, and cultured using 5 L-jar under the conditions of 30 ° C., 300 rpm (stirring rotation), 0.5 L / min (Air). The composition of the production medium was 3% soybean oil, 0.02% MgSO ₄ .7H ₂ O, 0.02% KH ₂ PO ₄ , and 0.1% yeast extract. 250 ml of the culture solution was centrifuged (6500 rpm, 30 min), the supernatant was removed, and the precipitate (bacteria) was collected. 50 ml of ethyl acetate was added to the precipitate, and after sufficient stirring, the mixture was centrifuged (8500 rpm, 30 min), separated into a precipitate and a supernatant, and the supernatant was concentrated with an evaporator. Using silica gel, eluting with chloroform: acetone = 1: 0, chloroform: acetone = 9: 1, chloroform: acetone 1: 1, chloroform: acetone = 3: 7, chloroform: acetone = 0: 1, and MEL-A and A triacyl MEL-A fraction was obtained.

Example 2 Production of MEL-B and triacyl MEL-B
0.2 ml of Pseudozyma tsukubaensis frozen stock was inoculated into a 20 ml YM medium / 500 ml Sakaguchi flask and cultured overnight at 26 ° C., 180 rpm, and used as an inoculum. 0.2 ml of the inoculum was again inoculated into a 20 ml YM seed medium / 500 ml Sakaguchi flask and cultured overnight at 26 ° C. and 180 rpm to obtain an inoculum. 20 ml of inoculum was inoculated into 2 L of YM medium / 5 L Jar and cultured at 26 ° C., 300 rpm (1/4 VVM, 0.5 L air / min) for 8 days. The culture solution was centrifuged at 7,900 rpm for 60 minutes at 4 ° C. to separate the cells (including MEL-B) and the supernatant. 80 ml of ethyl acetate was added to each bacterial cell fraction, and the mixture was stirred up and down so that the bacterial cells were sufficiently suspended, and then centrifuged at 4,900 rpm for 30 minutes at 4 ° C. An equal amount of saturated saline was added to the obtained supernatant and stirred to obtain an ethyl acetate layer. An appropriate amount of anhydrous sodium sulfate was added to the ethyl acetate layer and the mixture was allowed to settle for 30 minutes and then evaporated to obtain a crude MEL-B product. The obtained MEL-B crude purified product was eluted with hexane: acetone = 5: 1 and hexane: acetone = 1: 1 using a silica gel column to obtain MEL-B and triacyl MEL-B fraction purified products.

Example 3 Preparation of MEL-C and triacyl MEL-C
0.2 ml of Pseudozyma hubeiensis frozen stock was inoculated into a 20 ml YM seed medium / 500 ml Sakaguchi flask and cultured overnight at 26 ° C., 180 rpm, and used as a seed seed. 0.2 ml of the inoculum was again inoculated into a 20 ml YM seed medium / 500 ml Sakaguchi flask and cultured overnight at 26 ° C. and 180 rpm to obtain an inoculum. 20 ml of inoculum was inoculated into 2 L of YM medium / 5 L Jar and cultured at 26 ° C., 300 rpm (1/4 VVM, 0.5 L air / min) for 8 days. The culture solution was centrifuged at 7,900 rpm for 60 minutes at 4 ° C. to separate the cells (including MEL-C) and the supernatant. 80 ml of ethyl acetate was added to each bacterial cell fraction, and the mixture was stirred up and down so that the bacterial cells were sufficiently suspended, and then centrifuged at 4,900 rpm for 30 minutes at 4 ° C. An equal amount of saturated saline was added to the obtained supernatant and stirred to obtain an ethyl acetate layer. An appropriate amount of anhydrous sodium sulfate was added to the ethyl acetate layer and the mixture was allowed to settle for 30 minutes and then evaporated to obtain a crude MEL-B product. The obtained MEL-C crude product was eluted using a silica gel column with heptane: ethyl acetate = 1: 1, heptane: ethyl acetate = 1: 2, heptane: ethyl acetate = 1: 3, and MEL-C and triacyl. A MEL-C fraction purified product was obtained.

Example 4 Production of MEL Using Olive Oil In the production of MEL in Example 1, soybean oil was used as a raw material for production. MEL-B and MEL-C are isolated and purified. The MEL fraction obtained at this time is referred to as MEL-A (OL), MEL-B (OL), and MEL-C (OL) to distinguish them from the MEL of Example 1.

Example 5 Mannosyl mannitol lipid (MML) production Pseudozyma parantarctica JCM 11752 strain was cultured. That is, Pseudozyma parantarctica JCM 11752 strain preserved in a preservation medium (malt extract 3 g / L, yeast extract 3 g / L, peptone 5 g / L glucose 10 g / L, agar 30 g / L) In a test tube containing 2 mL of liquid medium having a composition of glucose 20 g / L, yeast extract 1 g / L, sodium nitrate 3 g / L, potassium dihydrogen phosphate 0.3 g / L, and magnesium sulfate 0.3 g / L. One platinum loop was inoculated and cultured with shaking at 30 ° C. for 1 day. Subsequently, the obtained bacterial cell culture solution was mixed with olive oil 50 g / L as a vegetable oil, mannitol 100 g / L as a sugar alcohol, yeast extract 1 g / L, sodium nitrate 3 g / L, potassium dihydrogen phosphate 0.3 g / L. And a Sakaguchi flask containing 30 mL of a liquid medium having a composition of magnesium sulfate 0.3 g / L, and cultured at 34 ° C. for 7 days. The above culture broth was collected, the mannosyl alditol lipid in the culture broth was extracted with ethyl acetate, purified by silica gel chromatography, and the structure of the product was analyzed by ¹³ C NMR and ¹ H NMR to confirm the structure.

Example 6 Production of mannosyl arabitol lipid (MAraL) MAraL was produced by culturing in the same manner as in Example 5 except that culture in Example 5 was carried out in a medium containing 100 g / L of arabitol as a sugar alcohol.

Example 7 Production of Mannosyl Sorbitol Lipid (MSL) MSL was produced by culturing in the same manner as in Example 5 except that the culture of Example 5 was carried out in a medium containing 100 g / L of sorbitol as a sugar alcohol.

Example 8 Production of mannosyl ribitol lipid (MRL) MRL was produced by culturing in the same manner as in Example 5 except that cultivation of Example 5 was carried out in a medium containing 100 g / L of ribitol as a sugar alcohol.

(Example 9) Preparation of shampoos Shampoos shown in Tables 1 to 4 were prepared according to a conventional method.

Example 10 Test of slipperiness, moistness and suppleness 20 g (length 15 cm) of healthy Japanese female hair was bundled, and 1 g of the shampoo of Tables 1 to 4 was applied to this hair bundle and whipped for 1 minute. After rinsing with running water for 30 seconds, the moisture was wiped off with a towel and dried with a dryer. The “smooth feeling”, “moist feeling” and “smoothness” of dry hair were subjected to sensory evaluation according to the following criteria.

The sensory evaluation was scored by five panels (evaluators) as follows, and the total was obtained as an evaluation score. Based on this, the following criteria were used.

《Each panel score》
Slip feeling:
3: There is a good natural slip.
2: There is slip.
1: Neither can be said.
0: There is a squeak.

Moist feeling:
3: Very moist.
2: Moist.
1: Neither can be said.
0: Not moist.

Suppleness:
3: Very supple.
2: It is supple.
1: Neither can be said.
0: Not supple.

<Criteria>
: Total 15-11 points
○: Total 10-6 points
Δ: Total 5 to 1 point ×: Total 0 point

(Usability test results)
As a result of comparing the usability of Formulation Examples 1 to 31 and Comparative Examples 1 to 6 of the present invention, the present invention can impart a good feel to the hair such as a slipperiness, a moist feeling, and the natural suppleness of healthy hair. It became clear that familiarity with the skin was improved.

The hair cleansing agent of the present invention protects hair from physical and chemical irritation, and can impart good feel such as slipperiness, moistness, and suppleness of healthy hair to these hairs. It is expected to make a significant contribution to the industry through damage prevention and repair effects.

Claims (6)

A hair cleanser containing the following components (A) to (C).
(A) Biosurfactant
(B) Anionic surfactant
(C) Organic acid or inorganic acid or salt thereof   The hair cleansing agent according to claim 1, wherein the biosurfactant has a mannose skeleton.   The hair detergent according to claim 1 or 2, wherein the biosurfactant has a sugar alcohol linked to a glycoside bond to the hydroxyl group at the 1-position of the mannose skeleton. The biosurfactant having a mannose skeleton was selected from the group consisting of mannosyl erythritol lipid (MEL), mannosyl mannitol lipid (MML), mannosyl sorbitol lipid (MSL), mannosyl arabitol lipid (MAraL) and mannosyl ribitol lipid (MRL). The hair cleanser according to claim 3, wherein the hair cleanser is one or more compounds. MEL is mannosyl erythritol lipid A (MEL-A), mannosyl erythritol lipid B (MEL-B), mannosyl erythritol lipid C (MEL-C), mannosyl erythritol lipid D (MEL-D), triacyl derivative of MEL-A, The hair cleansing agent according to claim 4, which is one or more compounds selected from the group consisting of MEL-B triacyl, MEL-C triacyl and MEL-D triacyl. The hair detergent according to any one of claims 1 to 5, wherein the biosurfactant contains a saturated fatty acid and / or an unsaturated fatty acid.