JP2010254658A - Method for producing antibacterial ionized metal composition - Google Patents
Method for producing antibacterial ionized metal composition Download PDFInfo
- Publication number
- JP2010254658A JP2010254658A JP2009122508A JP2009122508A JP2010254658A JP 2010254658 A JP2010254658 A JP 2010254658A JP 2009122508 A JP2009122508 A JP 2009122508A JP 2009122508 A JP2009122508 A JP 2009122508A JP 2010254658 A JP2010254658 A JP 2010254658A
- Authority
- JP
- Japan
- Prior art keywords
- antibacterial
- metal
- silver
- ionized
- mixture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000203 mixture Substances 0.000 title claims abstract description 82
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 56
- 239000002184 metal Substances 0.000 title claims abstract description 47
- 229910052751 metal Inorganic materials 0.000 title claims abstract description 47
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 19
- 229910052709 silver Inorganic materials 0.000 claims abstract description 68
- 239000004332 silver Substances 0.000 claims abstract description 68
- 239000010949 copper Substances 0.000 claims abstract description 23
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims abstract description 18
- 229910052802 copper Inorganic materials 0.000 claims abstract description 18
- 238000000034 method Methods 0.000 claims abstract description 15
- 150000007524 organic acids Chemical class 0.000 claims abstract description 8
- 241000894006 Bacteria Species 0.000 claims description 40
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 36
- 238000000855 fermentation Methods 0.000 claims description 26
- 230000004151 fermentation Effects 0.000 claims description 26
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 19
- 239000002994 raw material Substances 0.000 claims description 15
- 241000228212 Aspergillus Species 0.000 claims description 12
- 239000004310 lactic acid Substances 0.000 claims description 12
- 235000014655 lactic acid Nutrition 0.000 claims description 12
- 239000008213 purified water Substances 0.000 claims description 12
- 240000006439 Aspergillus oryzae Species 0.000 claims description 8
- 235000002247 Aspergillus oryzae Nutrition 0.000 claims description 8
- 150000001720 carbohydrates Chemical class 0.000 claims description 6
- 241000186660 Lactobacillus Species 0.000 claims description 4
- 241000194017 Streptococcus Species 0.000 claims description 4
- 229940039696 lactobacillus Drugs 0.000 claims description 4
- 241001513093 Aspergillus awamori Species 0.000 claims description 3
- 241000228245 Aspergillus niger Species 0.000 claims description 3
- 230000032683 aging Effects 0.000 claims description 3
- 238000000605 extraction Methods 0.000 abstract description 2
- 229910021645 metal ion Inorganic materials 0.000 abstract description 2
- 238000001914 filtration Methods 0.000 abstract 1
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 65
- 239000000243 solution Substances 0.000 description 11
- 239000000843 powder Substances 0.000 description 10
- 230000001580 bacterial effect Effects 0.000 description 8
- 239000012085 test solution Substances 0.000 description 8
- 239000004744 fabric Substances 0.000 description 7
- 239000004033 plastic Substances 0.000 description 7
- 229920003023 plastic Polymers 0.000 description 7
- 239000003242 anti bacterial agent Substances 0.000 description 6
- 241000191967 Staphylococcus aureus Species 0.000 description 5
- 239000003599 detergent Substances 0.000 description 5
- 229920001817 Agar Polymers 0.000 description 4
- 238000010521 absorption reaction Methods 0.000 description 4
- 239000008272 agar Substances 0.000 description 4
- 230000000845 anti-microbial effect Effects 0.000 description 4
- 239000000835 fiber Substances 0.000 description 4
- JPVYNHNXODAKFH-UHFFFAOYSA-N Cu2+ Chemical compound [Cu+2] JPVYNHNXODAKFH-UHFFFAOYSA-N 0.000 description 3
- 241000588653 Neisseria Species 0.000 description 3
- 229910001431 copper ion Inorganic materials 0.000 description 3
- 238000005520 cutting process Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 150000002739 metals Chemical class 0.000 description 3
- 229920002635 polyurethane Polymers 0.000 description 3
- 239000004814 polyurethane Substances 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- 229920000742 Cotton Polymers 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 2
- 239000004594 Masterbatch (MB) Substances 0.000 description 2
- 239000004698 Polyethylene Substances 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000003385 bacteriostatic effect Effects 0.000 description 2
- 230000033228 biological regulation Effects 0.000 description 2
- 238000012136 culture method Methods 0.000 description 2
- 238000007865 diluting Methods 0.000 description 2
- 239000003792 electrolyte Substances 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 2
- 229910052753 mercury Inorganic materials 0.000 description 2
- 239000002504 physiological saline solution Substances 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000012113 quantitative test Methods 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 235000000346 sugar Nutrition 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 241000589220 Acetobacter Species 0.000 description 1
- 241000228257 Aspergillus sp. Species 0.000 description 1
- 229920001661 Chitosan Polymers 0.000 description 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 229910001361 White metal Inorganic materials 0.000 description 1
- 238000003321 atomic absorption spectrophotometry Methods 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000003749 cleanliness Effects 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000011067 equilibration Methods 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000004898 kneading Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 238000005375 photometry Methods 0.000 description 1
- 229920000728 polyester Polymers 0.000 description 1
- -1 polyethylene Polymers 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 238000012805 post-processing Methods 0.000 description 1
- OGFYIDCVDSATDC-UHFFFAOYSA-N silver silver Chemical compound [Ag].[Ag] OGFYIDCVDSATDC-UHFFFAOYSA-N 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 239000010969 white metal Substances 0.000 description 1
Images
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Description
この発明は、イオン化金属組成物の製造方法に関するものであり、一層詳細には、例えば、衣類などの製品に抗菌機能を付与する際に好適に使用される抗菌性イオン化金属組成物の製造方法に関するものである The present invention relates to a method for producing an ionized metal composition, and more particularly, to a method for producing an antibacterial ionized metal composition that is suitably used for imparting an antibacterial function to a product such as clothing. Is a thing
近時、清潔を志向する意識の高まりなどから抗菌処理を施した各種の生活用品、衣料品などが種々開発され、その販売量も増大しており有望な市場として注目されている。 Recently, various items of daily use and clothing that have undergone antibacterial treatment have been developed due to the increasing awareness of cleanliness, and their sales volume has increased, attracting attention as a promising market.
ところで、この種の抗菌製品における抗菌手段として一般的には化学合成した抗菌剤が使用されており、この抗菌剤を所望の製品に噴霧したり、あるいは該抗菌剤を付着させた繊維を使用することによりその製品に抗菌機能を付与している。
しかしながら、洗濯、クリーニングなどにより抗菌剤が剥離、脱落することが多く、その結果、抗菌機能が急速に低下してしまうことが指摘されている。
このため、例えば、化学合成した抗菌剤を直接練りこんだ繊維や蟹の甲羅から抽出したキトサンなどの天然抗菌物質を練りこんだ繊維などを使用することにより抗菌性能を長期に亘って維持できるようにする提案もなされてはいるが、製造が面倒でコストも嵩むだけでなく、通常よりも多少高く設定されている製品価格がさらに上昇してしまうという問題があった。By the way, a chemically synthesized antibacterial agent is generally used as an antibacterial means in this type of antibacterial product, and the antibacterial agent is sprayed on a desired product or a fiber to which the antibacterial agent is attached is used. The antibacterial function is given to the product.
However, it has been pointed out that antibacterial agents often peel and fall off due to washing, cleaning, etc., and as a result, the antibacterial function rapidly decreases.
For this reason, for example, it is possible to maintain antibacterial performance over a long period of time by using a fiber directly kneaded with a chemically synthesized antibacterial agent or a fiber kneaded with a natural antibacterial substance such as chitosan extracted from the shell of straw. However, there is a problem that not only the production is troublesome and the cost is increased, but also the price of the product set a little higher than usual is further increased.
ところで、抗菌性の高い金属としては水銀Ｈｇ、鉛Ｐｂ、銀Ａｇ、銅Ｃｕなどが知られているが、これらのうち水銀Ｈｇ、鉛Ｐｂは人や動物さらには環境に対しても問題があるため実際に使用することはできない。
一方、銀Ａｇや銅Ｃｕは金属の中でも電気伝導率と熱伝導率が高く、銀Ａｇは可視光線の反射率も高いことから「しろがね（白銀；白い金属）」とも呼ばれており、いずれもバクテリアなどに対して極めて強い殺菌力を有しているため、例えば、浄水器の殺菌装置などの抗菌手段として広く使用されている。By the way, mercury Hg, lead Pb, silver Ag, copper Cu and the like are known as metals having high antibacterial properties. Of these, mercury Hg and lead Pb have problems for humans, animals and the environment. Therefore, it cannot actually be used.
On the other hand, silver Ag and copper Cu are also called “shirogane (white silver; white metal)” because silver and silver Cu have high electrical conductivity and thermal conductivity, and silver Ag also has high reflectance of visible light. Since it has extremely strong sterilizing power against bacteria and the like, it is widely used as an antibacterial means such as a water purifier sterilizer.
このような事情から、例えば、微粉末の銀を水中で帯電するように浮遊させたコロイド状態の銀溶液を病原菌の殺菌・滅菌に使用したり、あるいは衣料品の抗菌手段として使用することも提案されているが（特許文献１）、微粉末の銀といってもせいぜいサブミクロン程度のサイズであるため、イオンとして抗菌性を備えていたとしても充分な抗菌機能を獲得できないだけでなく、剥離、脱落など耐久性の点で相変わらず問題があり、費用対効果の面からも満足できるものとは言えなかった。 Under such circumstances, for example, it is also proposed to use a colloidal silver solution in which fine powdered silver is suspended so as to be charged in water for sterilization and sterilization of pathogens or as an antibacterial means for clothing. (Patent Document 1) However, even if it is a fine powder of silver, it is at most a submicron size, so even if it has antibacterial properties as an ion, it can not only acquire a sufficient antibacterial function, but also peels off. However, there were still problems in terms of durability, such as dropout, and it could not be said that it was satisfactory in terms of cost effectiveness.
そこで、本発明では、抗菌機能に優れた金属をイオンとして有効に利用することができる安全なイオン化金属組成物の製造方法を提供することを課題とするものである。 Accordingly, an object of the present invention is to provide a safe method for producing an ionized metal composition that can effectively use a metal having an antibacterial function as ions.
本発明ではこの課題を解決するため、抗菌性を備える原料としての金属を適宜の手段により微細化し、この微細化した金属を一次醗酵させのち再び醗酵させ、ついでこの二次醗酵混合物を熟成することにより有機酸と抗菌性を備える金属が解離（電解質状態）している状態のイオン化金属組成物として製造するものである。 In the present invention, in order to solve this problem, the metal as a raw material having antibacterial properties is refined by an appropriate means, the refined metal is first fermented and then fermented again, and then the secondary fermentation mixture is aged. Is produced as an ionized metal composition in a state where an organic acid and a metal having antibacterial properties are dissociated (electrolyte state).
この場合、抗菌性を備える微細化した金属として、銀または銀および銅を使用するのが好適である。 In this case, it is preferable to use silver or silver and copper as the refined metal having antibacterial properties.
より具体的には、微細化した銀または銀および銅原料に所定量の浄化水を加えて蒸煮し、つぎにこれを所定温度に保持するとともにアスペルギルス属の麹菌１０重量％〜３０重量％と糖質を複数回加えることにより一次醗酵させ、この一次発酵金属混合物にストレプトコックス属またはラクトバチルス属の乳酸菌、酢酸菌を単独でまたはこれらの混合物１０重量％〜２０重量％と糖質を複数回加えて所定温度に保持することにより二次醗酵させ、次いで二次発酵金属混合物を３５℃〜４０℃で熟成することにより乳酸菌や酢酸菌から生じた有機酸中に銀または銀および銅を解離し、さらにこれを濾過抽出することを特徴とするものである。 More specifically, a predetermined amount of purified water is added to the refined silver or silver and copper raw material and steamed. Next, this is kept at a predetermined temperature and 10% to 30% by weight of Aspergillus spp. The primary fermentation is performed by adding the quality multiple times, and the lactic acid bacteria of the genus Streptococcus or Lactobacillus and the acetic acid bacteria are added to the primary fermented metal mixture alone or 10% to 20% by weight of these mixtures and the saccharide is added several times. Second fermentation by holding at a predetermined temperature, and then aging the secondary fermentation metal mixture at 35 ° C. to 40 ° C. to dissociate silver or silver and copper into the organic acid generated from lactic acid bacteria and acetic acid bacteria, Furthermore, this is filtered and extracted.
この場合、アスペルギルス属の麹菌としては、アスペルギルス・オリゼー、アスペルギルスソーエ、アスペルギルス・ニガー、アスペルギルス・アワモリ、アスペルギルス・レベンスを単独でまたはこれらの混合物を使用するのが好適である。 In this case, it is preferable to use Aspergillus oryzae, Aspergillus soe, Aspergillus niger, Aspergillus awamori, Aspergillus revens alone or a mixture thereof as the Aspergillus genus.
また、二次醗酵金属混合物の熟成に際しては、遠赤外線照射およびエレクトロン供給雰囲気において流動させながら行うのが好ましい。 The secondary fermentation metal mixture is preferably aged while flowing in a far-infrared irradiation and electron supply atmosphere.
さらに、本発明は上述の方法により得られたイオン化金属組成物も包含するものである。 Further, the present invention includes an ionized metal composition obtained by the above-described method.
本発明に係るイオン化金属組成物の製造方法によれば；
古くから行われている発酵技術を利用して微細化金属を一次醗酵させたのち二次醗酵させ、この二次醗酵混合物に含まれる金属を有機酸中に解離させるので安全に且つ簡便にイオン化金属組成物の製造することができるものである。According to the method for producing an ionized metal composition according to the present invention;
The fermented technology that has been used for a long time has been used for primary fermentation of the refined metal, followed by secondary fermentation, and the metals contained in this secondary fermentation mixture are dissociated into organic acids, making it safe and simple to ionize metals. The composition can be manufactured.
また、微細化した金属原料に所定量の浄化水を加えて蒸煮し、つぎにこれを所定温度に保持するとともにアスペルギルス属の麹菌１０重量％〜３０重量％と糖質を複数回加えることにより一次醗酵させ、この一次発酵金属混合物にストレプトコックス属またはラクトバチルス属の乳酸菌、酢酸菌を単独でまたはこれらの混合物１０重量％〜２０重量％と糖質を複数回加えて所定温度に保持することにより二次醗酵させ、次いで二次発酵金属混合物を３５℃〜４０℃で熟成して濾過抽出するので、二次醗酵で生じたに乳酸、酢酸などの有機酸と金属が解離している電解質の状態として得られ、従って、金属イオンが保有している抗菌性を充分利用することができる。 In addition, a predetermined amount of purified water is added to the refined metal raw material and steamed. Next, the mixture is kept at a predetermined temperature, and 10% to 30% by weight of Aspergillus sp. By fermenting, and adding the lactic acid bacteria of the genus Streptococcus or Lactobacillus, acetic acid bacteria alone or 10% -20% by weight of these mixtures and saccharides to the primary fermented metal mixture a plurality of times and maintaining at a predetermined temperature. Since the secondary fermentation metal mixture is aged at 35 to 40 ° C. and filtered and extracted, the state of the electrolyte in which the organic acid such as lactic acid and acetic acid is dissociated from the metal produced in the secondary fermentation Therefore, the antibacterial property possessed by the metal ions can be fully utilized.
なお、本発明で使用する金属としての銀または銀および銅が保有する抗菌性を充分利用できるのは、銀または銀および銅イオンが対象となる製品の組織表面および内部に取り込まれ、プラス荷電している銀または銀および銅イオンと通常マイナス荷電している細菌類とが結合して、所謂、電気的平衡作用により細菌類の増殖が抑制され、さらには、銀または銀および銅イオン自体が放出する遠赤外線の作用により殺菌されるからであり、従って、製品への後加工による抗菌性の付与も行うことができ、さらには、従来の抗菌剤による点効果に対して面としての抗菌効果を得ることができるなど種々の効果を奏するものである。 The antibacterial property possessed by silver or silver and copper as a metal used in the present invention can be fully utilized because silver or silver and copper ions are incorporated into the tissue surface and inside of the target product and positively charged. The silver or silver and copper ions that are normally bound to the negatively-charged bacteria are bound to suppress the growth of the bacteria by the so-called electrical equilibration action, and the silver or silver and copper ions themselves are released. This is because it is sterilized by the action of far-infrared rays. Therefore, antibacterial property can be imparted to the product by post-processing, and furthermore, the antibacterial effect as a surface against the point effect by the conventional antibacterial agent is achieved. It is possible to obtain various effects.
次に、本発明に係るイオン化金属組成物の製造方法における実施の形態としてイオン化銀組成物の製造方法を例示し、以下詳細に説明する。
図１において、本発明に係るイオン化銀組成物の製造方法においては、まず、従来公知の手段により微細粉末に加工した原料銀（微細粉末銀）１０を用意し、この原料粉末銀１０の重量に対して８倍量〜１５倍量の水、好ましくは、上水から塩素などの不純物を除去した浄化水１２を混合して１０分程度煮沸したのち、３５℃〜４０℃まで自然冷却する。この場合、原料粉末銀の粒径としては次段の醗酵操作の効率を勘案すると細かいほど良いのは勿論であるが、好ましくは、サブミクロン程度の粉末銀が好適に使用される。Next, a method for producing an ionized silver composition is illustrated as an embodiment in the method for producing an ionized metal composition according to the present invention, and will be described in detail below.
In FIG. 1, in the method for producing an ionized silver composition according to the present invention, first, raw material silver (fine powder silver) 10 processed into a fine powder by a conventionally known means is prepared, and the weight of the raw
次に煮沸処理した原料粉末銀１０と浄化水１２の混合物１４に対して１０重量％〜３０重量％のアスペルギルス属の麹菌１６と、この麹菌１６と略同量の糖質１８を加えて加熱ヒータなどで３５℃〜４０℃に保持することにより一次醗酵を行う。 Next, 10 wt% to 30 wt% of Aspergillus gonococcus 16 and a
なお、混合物１４に加えるアスペルギルス属の麹菌１６としては、例えば、アスペルギルス・オリゼー、アスペルギルスソーエ、アスペルギルス・ニガー、アスペルギルス・アワモリ、アスペルギルス・レベンスなどを単独でまたはこれらの混合物を使用することができる。
また、この一次醗酵の際、銀の抗菌作用により当初は麹菌の一部が死滅したり弱ってしまい良好な醗酵を得られないことがあるので保持温度や醗酵状態を常時観察しながら、麹菌１６と糖質１８を複数回加えて調整し確実に醗酵させが、混合物１４に加える麹菌１６の量が１０重量％未満であると量的な関係から充分な醗酵を期待できず、また３０重量％を超えると量が多すぎて経済性が低下することになるのでこの点にも留意しながら醗酵を行う。In addition, as the Aspergillus gonococcus 16 added to the
Further, during this primary fermentation, some of the koji molds may initially be killed or weakened due to the antibacterial action of silver, and a good fermentation may not be obtained. And
麹菌１６などの混合により得られた一次醗酵銀混合物２０には、この一次醗酵銀混合物２０の５重量％程度の糖質１８を新たに補填するとともにストレプトコックス属またはラクトバチルス属の乳酸菌、アセトバクター属の酢酸菌を単独でまたはこれらの混合物２２を１０重量％〜２０重量％加えて、再び３５℃〜４０℃で１０日ほど保持することにより二次醗酵させる。
なお、この一次醗酵銀混合物２０に加える乳酸菌、酢酸菌あるいはこれらの混合物２２が５重量％未満であると醗酵が充分行われず、また２５重量％を超えると量が多すぎて経済性が低下してしまうことになる。
さらには、この二次醗酵の際も乳酸菌、酢酸菌あるいはこれらの混合物２２の一部が一次醗酵時と同様に死滅したり弱ってしまうことのないようその保持温度や醗酵状態を観察しながら、糖質１８および乳酸菌、酢酸菌またはこれらの混合物２２を複数回加えて調整を行うことにより確実に二次醗酵させる。The primary fermented
If the lactic acid bacteria, acetic acid bacteria or their
Furthermore, during this secondary fermentation, while observing the holding temperature and fermentation state so that lactic acid bacteria, acetic acid bacteria or a part of these
このようにして得られた二次醗酵銀混合物２４は、適宜の加熱ヒータなどを使用して３５℃〜４０℃に保持して熟成を行う。
この場合、３５℃〜４０℃に保持した二次醗酵銀混合物２４は、好ましくは、遠赤外線照射下および静電磁場、エレクトロン（−ｅ）供給雰囲気において撹拌しながら流動させることによりゆっくりと熟成し、原料としての粉末銀１０の液化を促進して二次醗酵によって乳酸菌、酢酸菌あるいはこれらの混合物２２から生じた乳酸、酢酸などの有機酸中に銀Ａｇを解離（イオン化）させる。The secondary fermented
In this case, the secondary fermented
そして、二次醗酵銀混合物２４中の銀Ａｇが充分に解離したら、２〜３週間程静置したのち紫外線などにより殺菌し、イオン化銀組成物２６として濾過抽出する。
この抽出によって得られたイオン化銀組成物２６は、常温で略透明を呈し、原子吸光光度法による分析試験（財団法人 日本食品分析センター）をしたところ１００ｍｌ中に銀Ａｇを５９２ｍｇ含有していた。なお、上述の本発明に係る製造方法で得られるイオン化銀組成物における銀Ａｇの含有率は概ね０．４％〜０．６％（４００ｍｇ〜６００ｍｇ）の範囲であった。And if silver Ag in the secondary fermented
The
また、上述した実施の形態であるイオン化銀組成物の製造方法において、原料としての金属である微細粉末銀に代替して微細粉末銅を使用してイオン化銅組成物を製造し、得られたにイオン化銅組成物を原子吸光光度法による分析試験（財団法人 日本食品分析センター）をしたところ１００ｍｌ中に銅Ｃｕを１４５０ｍｇ含有していた。なお、得られたイオン化銅組成物における銅Ｃｕの含有率は概ね１％〜２％（１０００ｍｇ〜２０００ｍｇ）の範囲であった。 Moreover, in the manufacturing method of the ionized silver composition which is embodiment mentioned above, it replaced with the fine powder silver which is a metal as a raw material, manufactured the ionized copper composition using fine powder copper, and obtained. When the ionized copper composition was subjected to an atomic absorption spectrophotometric analysis test (Japan Food Analysis Center), 1450 mg of copper Cu was contained in 100 ml. In addition, the content rate of copper Cu in the obtained ionized copper composition was in the range of approximately 1% to 2% (1000 mg to 2000 mg).
実験例１（生地の加工）
まず、浄化水にイオン化銀銀組成物２６を０．２％添加し、この浄化水（４０℃）にポリエステル製の生地（白）を投入して２時間流水加工したのち乾燥し、原子吸光光度法により分析試験（財団法人 日本食品分析センター）をしたところ、銀Ａｇを１０．７ｐｐｍ含有していた。
この実験結果によれば、本発明方法で得られたイオン化銀は簡単な流水加工によっても繊維組織に充分付着させることができることが確認された。 Experimental Example 1 (Dough processing)
First, 0.2% of ionized
According to this experimental result, it was confirmed that the ionized silver obtained by the method of the present invention can be sufficiently adhered to the fiber structure by simple flowing water processing.
実験例２（洗濯による抗菌性試験）
実験例１と同様に調製した浄化水（イオン化銀銀組成物２６を０．２％添加、４０℃）に綿生地製Ｔシャツを投入して２時間流水加工したのち乾燥した試験用原品を調製した。
次にＪＩＳ Ｌ ０２１７の１０３号で規定される洗濯（但し、ＪＡＦＥＴ標準洗剤を使用）を１０回行った原品試験布１と、洗濯を行わない原品試験布２、さらに比較のための無加工試験布３（標準綿布）を用意した。
ついで、各試験布１、２および無加工試験布３使用してＪＩＳ Ｌ １９０２、定量試験（菌液吸収法）による抗菌性の試験を行った。具体的には、各試験片１、２および無加工試験片３に試験菌として黄色ぶどう球菌（Ｓｔａｐｈｙｌｏｃｏｃｃｕｓａｕｒｅｕｓ ＡＴＣＣ ６５３８Ｐ）を植菌し、混釈平板培養法により生菌数の測定（財団法人日本紡績検査協会）で行ったところつぎのような結果を得た。 Experimental Example 2 (Antimicrobial test by washing)
A test original product that was dried after the cotton fabric T-shirt was put into purified water (0.2% of ionized
Next, the original test cloth 1 which was washed 10 times (provided using JAFET standard detergent) as defined in No. 103 of JIS L 0217, the original test cloth 2 which was not washed, A processed test cloth 3 (standard cotton cloth) was prepared.
Subsequently, each of the test cloths 1 and 2 and the unprocessed test cloth 3 was used to perform an antibacterial test by JIS L 1902 and a quantitative test (bacterial solution absorption method). Specifically, Staphylococcus aureus ATCC 6538P was inoculated as a test bacterium in each of test pieces 1 and 2 and unprocessed test piece 3, and the number of viable bacteria was measured by a pour plate culture method (Nippon Spinning Co., Ltd.). The following results were obtained when it was conducted at the Inspection Association.
［試験結果］
実験例３（プラスチックの抗菌性比較試験１）
１Ｋｇのプラスチック原料（ポリエチレン）に、本発明方法で得られたイオン化銀組成物２６を１００ｍｌ混錬して１０％濃度のマスターバッチを調製し、このマスターバッチ１に対して９のプラスチック原料（ポリエチレン）を混錬することにより「まな板用プラスチック原料」を調製した。
次にこの「まな板用プラスチック原料」の抗菌性試験（ＪＩＳ Ｚ ２８０１：２０００「抗菌加工製品−抗菌性試験方法・抗菌効果」準拠）を行った。
具体的には、普通寒天培地に２４時間培養した菌体（ＮＢＲＣ１２７３２黄色ブドウ球菌、ＮＢＲＣ３９７２大腸菌）を、蒸留水で１／５００に希釈した普通ブイヨン培地に、菌数が２．０×１０５〜１・０×１０６／ｍｌとなるよう懸濁し、その０．２ｍｌを検体（まな板用プラスチック原料）と対照（無加工試験）に滴下し、ＰＥフィルムを被せて３５℃で２４時間培養した。その後、ＳＣＤＰＬ培地１０ｍｌで洗い流し、寒天培地法（３５℃×２日）により生菌数を測定したところ、以下の結果を得た。 Experimental Example 3 (Antibacterial Comparative Test 1 Plastic)
A 1% plastic raw material (polyethylene) is kneaded with 100 ml of the ionized
Next, an antibacterial property test of this “cutting board plastic raw material” (based on JIS Z 2801: 2000 “Antimicrobial Processed Products—Antimicrobial Test Method / Antimicrobial Effect”) was performed.
Specifically, the cell number (NBRC12732 Staphylococcus aureus, NBRC3982 E. coli) cultured on a normal agar medium for 24 hours is diluted to 1/500 in distilled water to a normal bouillon medium, and the number of bacteria is 2.0 × 10 5 to The suspension was suspended at 1.0 × 10 6 / ml, 0.2 ml of the suspension was dropped onto a specimen (cutting board plastic raw material) and a control (non-processed test), covered with a PE film, and cultured at 35 ° C. for 24 hours. Thereafter, the cells were washed with 10 ml of SCDPL medium, and the viable cell count was measured by the agar medium method (35 ° C. × 2 days). The following results were obtained.
［試験結果］
実験例４（プラスチックの抗菌性試験）
１Ｋｇのプラスチック原料（ポリウレタン）に、本発明方法で得られたイオン化銀組成物２６を１０ｍｌ混錬して１％濃度のマスターバッチを調製し、このマスターバッチ１に対して９のプラスチック原料（ポリウレタン）を混錬して発泡することにより「インソール用発泡ポリウレタン」を調製した。
次に、このインソール用発泡ポリウレタン（試験体）を使用してＪＩＳ Ｌ １９０２、定量試験（菌液吸収法）による抗菌性の試験を行った。
具体的には、試験体に試験菌として黄色ぶどう球菌（Ｓｔａｐｈｙｌｏｃｏｃｃｕｓ ａｕｒｅｕｓ ＡＴＣＣ ６５３８Ｐ）を植菌し、混釈平板培養法により生菌数の測定を行ったところつぎのような結果を得た［注 試験菌液として、界面活性剤（Ｔｗｅｅｎ８０）０．０５％を添加した試験菌液を使用した］。 Experimental Example 4 (Plastic antibacterial test)
A master batch of 1% concentration was prepared by kneading 10 ml of the ionized
Next, using this foamed polyurethane for insole (test body), an antibacterial test was conducted by JIS L 1902, a quantitative test (bacterial solution absorption method).
Specifically, Staphylococcus aureus ATCC 6538P was inoculated as a test bacterium, and the number of viable bacteria was measured by the pour plate culture method, and the following results were obtained [Note: As the test bacterial solution, a test bacterial solution to which 0.05% of a surfactant (Tween 80) was added was used.
［試験結果］
実験例５（洗剤の抗菌力試験）
洗剤（新日本理化株式会社製；ＣＳ．Ａ２）に、本発明方法で得られたイオン化銀組成物０．３％とイオン化銅組成物０．３％を添加した検体Ａと、同洗剤に本発明方法で得られたイオン化銀組成物０．１５％とイオン化銅組成物０．１５％を添加した検体Ｂを調製し、これら検体Ａ、Ｂの細菌に対する抗菌力を財団法人日本食品分析センターにおいて試験を行った。
具体的には、普通寒天培地で３５℃±１℃、１８〜２４時間培養した試験菌株（ＮＢＲＣ１２７３２黄色ブドウ球菌、ＮＢＲＣ３９７２大腸菌）を生理食塩水に浮遊させ、菌数が１０７〜１０８／ｍｌとなるよう調製し、試験菌液とした。
つぎに精製水で調製した検体Ａおよび検体Ｂの１００倍希釈液１０ｍｌに試験菌液０．１ｍｌを夫々接種して試験液とした。これらの試験液を室温で保存し、２４時間後にＳＣＤＬ培地で直ちに１０倍に希釈して試験液中の生菌数を菌数測定用培地を用いて測定したところ以下の結果を得た（なお、試験液をＳＣＤＬ培地で１０倍に希釈することにより、検体に影響を受けずに生菌数が測定できることを予備試験により予め確認した）。 Experimental Example 5 (Detergent antibacterial activity test)
Specimen A obtained by adding 0.3% of ionized silver composition and 0.3% of ionized copper composition obtained by the method of the present invention to detergent (manufactured by Nippon Nippon Chemical Co., Ltd .; CS.A2), and this detergent Specimen B was prepared by adding 0.15% of the ionized silver composition and 0.15% of the ionized copper composition obtained by the inventive method, and the antibacterial activity of these specimens A and B against bacteria was determined by the Japan Food Analysis Center. A test was conducted.
Specifically, a test strain (NBRC12732 Staphylococcus aureus, NBRC3982 Escherichia coli) cultured on a normal agar medium at 35 ° C. ± 1 ° C. for 18-24 hours is suspended in physiological saline, and the number of bacteria is 10 7 to 10 8 / ml. To prepare a test bacterial solution.
Next, 0.1 ml of the test bacterial solution was inoculated into 10 ml of a 100-fold diluted solution of Sample A and Sample B prepared with purified water to prepare a test solution. These test solutions were stored at room temperature, and after 24 hours, immediately diluted 10-fold with SCDL medium and the number of viable bacteria in the test solution was measured using a medium for measuring the number of bacteria. The preliminary test confirmed that the number of viable bacteria could be measured without being affected by the specimen by diluting the
［試験結果］
実験例６（柔軟剤の抗菌力試験）
柔軟剤（新日本理化株式会社製；オアシス）に、本発明方法で得られたイオン化銀組成物０．３％とイオン化銅組成物０．３％を添加した検体Ａと、同柔軟剤に本発明方法で得られたイオン化銀組成物０．１５％とイオン化銅組成物０．１５％を添加した検体Ｂを調製し、これら検体Ａ、Ｂの細菌に対する抗菌力を財団法人日本食品分析センターにおいて試験を行った。
具体的には、普通寒天培地で３５℃±１℃、１８〜２４時間培養した試験菌株（ＮＢＲＣ１２７３２黄色ブドウ球菌、ＮＢＲＣ３９７２大腸菌）を生理食塩水に浮遊させ、菌数が１０７〜１０８／ｍｌとなるよう調製し、試験菌液とした。
つぎに精製水で調製した検体Ａおよび検体Ｂの１００倍希釈液１０ｍｌに試験菌液０．１ｍｌを夫々接種して試験液とした。これらの試験液を室温で保存し、２４時間後にＳＣＤＬ培地で直ちに１０倍に希釈して試験液中の生菌数を菌数測定用培地を用いて測定したところ以下の結果を得た（なお、試験液をＳＣＤＬ培地で１０倍に希釈することにより、検体に影響を受けずに生菌数が測定できることを予備試験により予め確認した）。 Experimental Example 6 (Antimicrobial test of softener)
Specimen A obtained by adding 0.3% of an ionized silver composition and 0.3% of an ionized copper composition obtained by the method of the present invention to a softener (manufactured by Shin Nippon Rika Co., Ltd .; Oasis) and this softener Specimen B was prepared by adding 0.15% of the ionized silver composition and 0.15% of the ionized copper composition obtained by the inventive method, and the antibacterial activity of these specimens A and B against bacteria was examined at the Japan Food Analysis Center. A test was conducted.
Specifically, usually 35 ° C. ± 1 ° C. in agar medium, 18 to 24 hour incubation with test strains (NBRC12732 Staphylococcus aureus, NBRC3972 coli) were suspended in physiological saline, the number of bacteria 10 7 ~10 8 / ml To prepare a test bacterial solution.
Next, 0.1 ml of the test bacterial solution was inoculated into 10 ml of a 100-fold diluted solution of Sample A and Sample B prepared with purified water to prepare a test solution. These test solutions were stored at room temperature, and after 24 hours, immediately diluted 10-fold with SCDL medium and the number of viable bacteria in the test solution was measured using a medium for measuring the number of bacteria. The preliminary test confirmed that the number of viable bacteria could be measured without being affected by the sample by diluting the
［試験結果］
１０・・原料銀（微細粉末銀）、
１２・・浄化水、
１４・・粉末銀と浄化水の混合物、
１６・・麹菌、または麹菌混合物、
１８・・糖質、
２０・・一次醗酵銀混合物、
２２・・乳酸菌、酢酸菌またはこれらの混合物、
２４・・二次醗酵銀混合物、
２６・・イオン化銀組成液、10. Raw material silver (fine powder silver),
12. Purified water,
14. Mixture of powdered silver and purified water,
16. .. Koji mold or Koji mold mixture,
18. Sugars,
20 ・ ・ Primary fermented silver mixture,
22. ・ Lactic acid bacteria, acetic acid bacteria or a mixture of these,
24 .. Secondary fermented silver mixture,
26 .. Ionized silver composition liquid,
Claims (6)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2009122508A JP5368170B2 (en) | 2009-04-24 | 2009-04-24 | Method for producing antibacterial ionized metal composition |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2009122508A JP5368170B2 (en) | 2009-04-24 | 2009-04-24 | Method for producing antibacterial ionized metal composition |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2010254658A true JP2010254658A (en) | 2010-11-11 |
| JP5368170B2 JP5368170B2 (en) | 2013-12-18 |
Family
ID=43316007
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2009122508A Active JP5368170B2 (en) | 2009-04-24 | 2009-04-24 | Method for producing antibacterial ionized metal composition |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP5368170B2 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2015101547A (en) * | 2013-11-22 | 2015-06-04 | 八藤 眞 | Method for producing ionization metal liquid which can bind to nutritional component |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998012924A1 (en) * | 1996-09-24 | 1998-04-02 | Snd Co., Ltd. | Method for preparing sterilizing solutions, sterilizing solutions, and sterilizing media |
| JP2002173405A (en) * | 2000-12-04 | 2002-06-21 | Hidehiro Tagusari | Sterilizing method with colloidal silver and sterilization material using the same |
| JP2006328532A (en) * | 2005-05-10 | 2006-12-07 | Samsung Electro-Mechanics Co Ltd | Metal nano particle, method for manufacturing and conductive ink |
| JP2007209324A (en) * | 2006-02-13 | 2007-08-23 | Makoto Yafuji | Method for producing iron supplement composition |
| WO2008003522A1 (en) * | 2006-07-05 | 2008-01-10 | Janssen Pharmaceutica N.V. | Method for producing metal nanoparticles |
| JP2008050682A (en) * | 2006-08-28 | 2008-03-06 | Makoto Yafuji | Method for improving silverwares |
-
2009
- 2009-04-24 JP JP2009122508A patent/JP5368170B2/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998012924A1 (en) * | 1996-09-24 | 1998-04-02 | Snd Co., Ltd. | Method for preparing sterilizing solutions, sterilizing solutions, and sterilizing media |
| JP2002173405A (en) * | 2000-12-04 | 2002-06-21 | Hidehiro Tagusari | Sterilizing method with colloidal silver and sterilization material using the same |
| JP2006328532A (en) * | 2005-05-10 | 2006-12-07 | Samsung Electro-Mechanics Co Ltd | Metal nano particle, method for manufacturing and conductive ink |
| JP2007209324A (en) * | 2006-02-13 | 2007-08-23 | Makoto Yafuji | Method for producing iron supplement composition |
| WO2008003522A1 (en) * | 2006-07-05 | 2008-01-10 | Janssen Pharmaceutica N.V. | Method for producing metal nanoparticles |
| JP2008050682A (en) * | 2006-08-28 | 2008-03-06 | Makoto Yafuji | Method for improving silverwares |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2015101547A (en) * | 2013-11-22 | 2015-06-04 | 八藤 眞 | Method for producing ionization metal liquid which can bind to nutritional component |
Also Published As
| Publication number | Publication date |
|---|---|
| JP5368170B2 (en) | 2013-12-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Paš et al. | Uptake of chromium (III) and chromium (VI) compounds in the yeast cell structure | |
| CN109536296B (en) | Antibacterial biological detergent for infants and preparation method thereof | |
| CN101979490B (en) | Cleaning agent for removing pesticides and microorganisms from surfaces of fruits and vegetables | |
| CN106083765B (en) | A kind of Environmental Safety cleaning agent with antibacterial functions | |
| KR20010095031A (en) | Silver Ion - Containing Solution and use thereof | |
| JP2010187648A (en) | Sterilization method using air plasma | |
| JP2008011795A (en) | Method for producing pu-erh tea | |
| EP2810559A1 (en) | Heated moist towelette and method for producing heated moist towelette | |
| JP5368170B2 (en) | Method for producing antibacterial ionized metal composition | |
| CN107232235A (en) | A kind of micro-nano shell water disinfectant and preparation method thereof | |
| CN103405378A (en) | Hand washing solution | |
| KR100817911B1 (en) | Composition for microbe-proof processing of fiber comprising silver nanoparticles and saponins, and a fiber microbe-proof processed with the same | |
| JP2007138343A (en) | Method for treatment of natural antimicrobial agent and antimicrobial fiber material | |
| CN110575816B (en) | Lactobacillus plantarum YW11 with high adsorption and high heavy metal lead tolerance | |
| CN109699866B (en) | Application of pterostilbene in inhibiting growth of aspergillus carbonarius and generation of ochratoxin A in grape juice | |
| CN106719816B (en) | Yttrium oxide-stalk cellulose composite Nano antibacterial material | |
| JP2002339243A (en) | Antibacterial treating agent and washing agent and antibacterial treating method | |
| CN108480647B (en) | Recycling method of nano-silver particle waste liquid | |
| CN111733025B (en) | Bacteriostatic laundry detergent for infants | |
| CN114907923A (en) | Preparation method of biological nano-silver sterilizing and disinfecting laundry tablets | |
| KR200404546Y1 (en) | Nano silver and perfume coating wrap | |
| KR20060068824A (en) | Nano silver and perfume contain door handle | |
| KR200379832Y1 (en) | Nano silver silver and perfume contain mobile phone portable hanger necklace | |
| CN114456882A (en) | Underwear laundry detergent containing yeast fermentation product extract | |
| Chen et al. | Sunlight-induced antibacterial poly (vinyl alcohol) grafted 3, 3′, 4, 4′-benzophenone tetracarboxylic dianhydride film for the preservation of Mongolian cheese |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20120224 |
|
| RD02 | Notification of acceptance of power of attorney |
Free format text: JAPANESE INTERMEDIATE CODE: A7422 Effective date: 20120628 |
|
| A977 | Report on retrieval |
Free format text: JAPANESE INTERMEDIATE CODE: A971007 Effective date: 20130208 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20130312 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20130513 |
|
| TRDD | Decision of grant or rejection written | ||
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20130827 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20130912 |
|
| R150 | Certificate of patent or registration of utility model |
Ref document number: 5368170 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| S111 | Request for change of ownership or part of ownership |
Free format text: JAPANESE INTERMEDIATE CODE: R313115 |
|
| R350 | Written notification of registration of transfer |
Free format text: JAPANESE INTERMEDIATE CODE: R350 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| S531 | Written request for registration of change of domicile |
Free format text: JAPANESE INTERMEDIATE CODE: R313531 |
|
| S111 | Request for change of ownership or part of ownership |
Free format text: JAPANESE INTERMEDIATE CODE: R313117 |
|
| S531 | Written request for registration of change of domicile |
Free format text: JAPANESE INTERMEDIATE CODE: R313531 |
|
| R350 | Written notification of registration of transfer |
Free format text: JAPANESE INTERMEDIATE CODE: R350 |