JP2010119329A - Pet food - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide pet food containing high unsaturated fatty acid-containing oil and fat, and having high preservation stability and palatability. <P>SOLUTION: The pet food contains the following ingredients (A), (B), and (C): (A) 6-50 mass% of oil and fat which has 20 mass% or more of unsaturated fatty acid containing at least two double bonds, in fatty acid composing oil and fat, (B) 0.0005-1 mass%, in terms of mineral content, of mineral ingredient selected from iron, an iron compound, copper, and a copper compound, in total, and (C) 0.7 mass% or more of citric acid and/or its salt, in total, in terms of citric acid content, and a pH of 3.5-7.0. <P>COPYRIGHT: (C)2010,JPO&INPIT

Description

  The present invention relates to a pet food containing a high content of unsaturated fatty acids.

  Due to the pet boom, the number of pets is increasing, and with it, diseases similar to human adult diseases such as pet obesity, diabetes and liver disease are increasing due to aging of pets, lack of exercise, overnutrition and the like. In addition, dogs and cats who become obese due to hormonal imbalance after contraceptive surgery are increasing. Many pet foods for preventing such obesity and weight gain have been developed. For example, a pet food containing a diacylglycerol-containing fat and oil having a high unsaturated fatty acid content has been proposed.

Such pet foods contain a large amount of unsaturated fatty acids, so that they are easily oxidized depending on the storage environment, and may affect odor, palatability and the like. In view of this, the present applicant, in a pet food with a high mineral content, includes a technology for blending vitamin C derivatives (Patent Document 1), a naturally occurring antioxidant, and the content of iron ions and copper ions in the pet food. It has been proposed to improve long-term storage stability by controlling technology (Patent Document 2).
JP 2005-204659 A JP 2007-110916 A

  The present invention relates to a pet food containing a high content of unsaturated fatty acids and having high storage stability and palatability.

  The present inventors contain oils and fats having a high unsaturated fatty acid content having two or more double bonds and containing mineral components, including metal salts, particularly copper and iron having a strong oxidation promoting action. As a result of studying the pet food to be used, as a result of containing 0.7% by mass or more of citric acid and / or a salt thereof and setting the pH of the pet food to 3.5 to 7, oxidation is remarkably suppressed. We found that palatability was good.

That is, the present invention includes the following components (A), (B) and (C),
(A) 6-50 mass% of fats and oils whose unsaturated fatty acid containing two or more double bonds is 20 mass% or more in the fatty acid which comprises fats and oils,
(B) Mineral component selected from iron, iron compound, copper and copper compound 0.0005 to 1% by mass in terms of mineral,
(C) Citric acid and / or a salt thereof A pet food having a total amount of 0.7% by mass or more in terms of citric acid and a pH of 3.5 to 7.0 is provided.
In addition, the present invention provides an oil and fat whose unsaturated fatty acid containing two or more double bonds is 20% by mass or more in the fatty acid constituting the oil and fat, and a mineral component selected from iron, iron compound, copper and copper compound. A method for producing a pet food containing 0.0005 to 1% by mass in terms of minerals, characterized in that citric acid and alkali metal salts of citric acid are combined in a total amount of 0.7% by mass or more in terms of citric acid. The present invention provides a method for producing pet food.

  The pet food of the present invention has a high anti-obesity effect and is remarkably excellent in long-term storage stability. Even when stored for a long period of time, changes in odor, lowering of palatability, etc. can be very effectively suppressed.

The pet food of the present invention contains (A) an oil and fat, and the oil and fat contains 20% by mass of an unsaturated fatty acid (hereinafter also simply referred to as a highly unsaturated fatty acid) containing two or more double bonds in the constituent fatty acid. (Hereinafter simply expressed as%). The content of polyunsaturated fatty acids in the constituent fatty acids is preferably 20 to 90%, more preferably 23 to 80%, and particularly preferably 25 to 70% from the viewpoint of anti-obesity effect and skin and hair health. Moreover, 2-45% of the content in the pet food of the fats and oils whose constituent fatty acid is a highly unsaturated fatty acid is preferable, 2.5-40% is more preferable, 3-35% is still more preferable. Here, as a highly unsaturated fatty acid, a C18-24 highly unsaturated fatty acid is mentioned, A linoleic acid, linolenic acid, EPA, DHA, and arachidonic acid are suitable.
In addition, as fatty acid which comprises fats and oils, the double bond may contain one unsaturated fatty acid and saturated fatty acid other than a highly unsaturated fatty acid. Here, examples of the unsaturated fatty acid having one double bond include unsaturated fatty acids having 16 to 24 carbon atoms, and oleic acid, palmitooleic acid and the like are preferable. Moreover, as a saturated fatty acid, a C12-C18 saturated fatty acid is mentioned, A palmitic acid, a stearic acid, etc. are suitable.

  The content of fats and oils in the pet food of the present invention is 6 to 50%, preferably 6 to 45%, more preferably 6 to 40%, more preferably 8 to 40% from the viewpoint of anti-obesity effect and removal of unpleasant odor of the pet food. 30% is particularly preferred.

  Examples of the oils and fats used in the pet food of the present invention include safflower oil, olive oil, cottonseed oil, corn oil, rapeseed oil, soybean oil, palm oil, sunflower oil, linseed oil, sesame oil, lard, beef tallow, fish oil, milk fat and the like However, the present invention is not limited to those blended as fats and oils, and includes oils and fats contained in other plant raw materials or animal raw materials.

  The oil and fat used in the pet food of the present invention can contain one or more of monoacylglycerol (MAG), diacylglycerol (DAG) and triacylglycerol (TAG). It is preferable to contain it from the point of obesity suppression effect. The content of DAG is preferably 10 to 85%, more preferably 20 to 80%, and particularly preferably 25 to 50% in fats and oils from the viewpoint of preventing obesity. Further, DAG is preferably 0.5 to 40%, more preferably 1 to 30%, and particularly preferably 1.5 to 25% in the pet food from the same viewpoint.

It is preferable that 80-100% of the constituent fatty acids are unsaturated fatty acids (UFA), more preferably 90-100%, more preferably 93-100%, especially 93-98%, especially 94-98%. It is preferable from the viewpoint of obesity prevention effect. Here, it is preferable that carbon number of this unsaturated fatty acid is 14-24, and also 16-22.
Among fatty acids constituting DAG, the content of linoleic acid is 15 to 65%, more preferably 20 to 60%, still more 30 to 55%, especially 35 to 50% in terms of obesity prevention effect and ingestion. preferable. Furthermore, from the viewpoint of oxidation stability and anti-obesity effect, the mass ratio of linoleic acid / oleic acid in DAG is 0.01 to 2.0, more preferably 0.1 to 1.8, and particularly 0.3 to 1. 7 is preferable.
Among fatty acids that constitute DAG, the content of linolenic acid is less than 15%, more preferably 0 to 13%, even more 1 to 10%, especially 2 to 9%. From the viewpoint of sex. As linolenic acid, α-linolenic acid and γ-linolenic acid are known as isomers, and α-linolenic acid is preferred.
In addition, when DAG is 100% in fats and oils components, it is preferable that the total amount of linoleic acid and linolenic acid is 20% or more in the fatty acid composition.

  Among fatty acids constituting DAG, the content of saturated fatty acid (SFA) is preferably less than 20%, more preferably 0 to 10%, more preferably 0 to 7%, particularly 2 to 7%, especially 2 to 6%. It is preferable from the viewpoints of obesity prevention effect, ingestion, and oxidative stability. As SFA, those having 14 to 24 carbon atoms and more preferably 16 to 22 carbon atoms are preferable, and palmitic acid and stearic acid are particularly preferable.

  Among fatty acids constituting DAG, the content of fatty acids having 12 or less carbon atoms is preferably 5% or less in terms of flavor and ingestion, and is further substantially 0 to 2%, particularly 0 to 1%. It is even more preferred not to. The remaining constituent fatty acids preferably have 14 to 24 carbon atoms, particularly 16 to 22 carbon atoms.

  Further, from the viewpoint of obesity prevention effect, diacylglycerol in which the ratio of 1,3-diacylglycerol in DAG is 50% or more, further 52 to 100%, further 54 to 90%, particularly 56 to 80% is used. Is preferred.

  DAG may contain what is contained in the above-mentioned natural fats and oils, but in order to adjust the content of DAG, the esterification reaction of fatty acids derived from fats and oils and glycerin, and fats and fats and glycerin It is preferable to mix what was obtained by transesterification etc.

  DAG is, for example, (1) oils and fats containing 20% or more of highly unsaturated fatty acids in the constituent fatty acids (eg, safflower oil, olive oil, cottonseed oil, corn oil, rapeseed oil, soybean oil, palm oil, sunflower oil, Fatty oil selected from linseed oil, sesame oil, lard, beef tallow, fish oil, milk fat, or fractionated oils thereof, randomized oil, hydrogenated oil, transesterified oil) and glycerin are hydroxylated with alkali metal or alkaline earth metal It can be transesterified in the presence of a product, or (2) it can be obtained by an esterification reaction of a fatty acid mixture having a highly unsaturated fatty acid content of 20% or more derived from these fats and oils with glycerin. At this time, two or more kinds of fats and oils may be mixed. These reactions can also be carried out by a chemical reaction using an alkali catalyst or the like as described above. However, the reaction is performed under mild conditions via an enzyme such as a 1,3-position selective lipase. It is preferable in terms of stability and palatability.

  The fats and oils in the pet food of the present invention ideally preferably have a DAG of 100%, but the fats and oils contain 10 to 85% of DAG, preferably as a main component, and further include TAG. Some MAG, free fatty acid, etc. may be contained. Here, the “main component” is a component having a maximum content contained in the fat or oil or a content comparable to it (more specifically, 0.7 times or more of the maximum content component). means. These include those contained in the fats and oils contained in the produced DAG-containing fats and oils, blended plant raw materials, or animal raw materials in addition to those contained in the natural fats and oils described above. In particular, the content of free fatty acids in fats and oils is preferably 20% or less in terms of ease of occurrence of unpleasant odors from fats and oils and in terms of palatability, more preferably 10% or less, further 5% or less, especially 2% or less. In particular, it is preferably 1% or less.

The pet food of the present invention also contains a mineral component selected from iron, an iron compound, copper and a copper compound as the component (B).
Examples of the iron compound include iron sulfate, ferric chloride, ferrous fumarate, ferrous carbonate, and iron oxide. The content of the iron compound in the pet food is within an acceptable range in pet food blending, 0.00001 to 1% in terms of iron, 0.00005 to 0.3%, particularly 0.0001 to 0.1%. Is preferred.

  Examples of the copper compound include copper sulfate, copper chloride, copper oxide, copper carbonate, copper sulfide, copper chlorophyll and the like. The content of the copper compound in the pet food is within an acceptable range in the pet food formulation, 0.00001 to 0.1% in terms of copper, 0.00005 to 0.03%, particularly 0.0001 to 0.00. 01% is preferred.

  Of these mineral components, iron or an iron compound and copper or a copper compound are preferably used in combination. In the present invention, the total content of these mineral components is 0.0005 to 1% in terms of mineral (iron and copper), more preferably 0.0005 to 0.5%, and 0.001 to 0.001. 0.1% is more preferable.

  The pet food of the present invention may contain manganese, cobalt, calcium, phosphorus, sodium, potassium and the like as a mineral component in addition to iron and copper. These mineral components are blended as, for example, manganese oxide, cobalt carbonate, calcium carbonate, calcium phosphate, sodium chloride, potassium chloride and the like.

  The pet food of the present invention further contains citric acid or a salt thereof as component (C), and the total amount is 0.7% by mass or more in terms of citric acid. Here, examples of the citrate include alkali metal salts and alkaline earth metal salts, and alkali metal salts are particularly preferable. The alkali metal salt means a normal salt of citric acid (trialkali metal salt), a monohydrogen dialkali metal salt, and a dihydrogen alkali metal salt. Examples of the alkali metal salt of citric acid include sodium salt and potassium salt, and sodium salt is particularly preferable. In the present specification, unless otherwise specified, sodium citrate represents trisodium citrate (dihydrate).

In addition, “in terms of citric acid” means that the component (C) contains citric acid and an alkali metal salt of citric acid (hereinafter also referred to as citric acid), or the component (C) is an alkali of citric acid. When it is only a metal salt, it means that the alkali metal content is replaced with hydrogen, and the mass is converted with the molecular weight of citric acid.
The pet food of the present invention contains citric acids in an amount of 0.7% by mass or more, further 0.75% by mass or more, particularly 1.0% by mass or more in terms of citric acid from the viewpoint of storage stability and palatability. It is preferable. The upper limit of the citric acid content is preferably 3.0% by mass, particularly 2.5% by mass in consideration of palatability and odor during storage.

  The pet food of the present invention has a pH of 3.5 to 7.0. Within this range, oxidation of the component (A) by the component (B) is effectively suppressed, and palatability is also good. It is preferable that the pH is 4.0 to 7.0, more preferably 4.5 to 6.8, since the effect of suppressing oxidation is further enhanced and the odor during storage is improved. In order to include citric acids and make the pH within such a range, there is a method of adding a pH adjusting agent in addition to citric acid. Among such methods, a method using citric acid and citrate in combination is preferable because it is easy to adjust the pH, has good palatability, and has a uniform taste. In particular, it is preferable to use the above-mentioned alkali metal citrate as the citrate.

In addition, "pH of pet food" means pH when pet food is measured by the following method.
<Method for measuring pH of pet food>
Qualitative filter paper (10 g of pet food) was added to 60 mL of ion exchange water at 45 ° C. (preferably ion exchange water having an electric conductivity of 0.01 mS / m or less at 25 ° C.) and allowed to stand for 1 hour. No. 1, manufactured by Advantec) and the pH of the filtrate was measured at 25 ° C.
In addition, when the property of pet food is solid, it is preferable to perform the said stirring operation after grind | pulverizing finely using a mortar etc.

  The pet food of the present invention preferably further contains a carbohydrate. Examples of the carbohydrate source include monosaccharides, oligosaccharides, polysaccharides, dietary fibers, and starches. Starches include waxy corn starch, corn starch, wheat starch, rice starch, glutinous rice starch, potato starch, honeydew starch, tapioca starch, sago starch, or those that have been chemically treated or chemically modified. Can be mentioned. Carbohydrates may be contained as cereals, and examples of cereals include wheat, rye, mica, rice, fin, awa, amarasansus, quinoa and the like. Carbohydrate content in pet food is 10 to 70%, more preferably 20 to 60%, especially 30 to 50%, which makes the health and beauty of the economical, anti-obesity effect, intake, stool condition and appearance healthy. It is preferable from the point.

In the pet food of the present invention, it is preferable that animal or vegetable protein is further included from the viewpoint of obesity prevention effect, ingestion, nutritional balance, and health and beauty. Sex proteins are particularly preferred. Examples of animal protein include milk protein such as casein, but animal meat protein is preferable from the viewpoint of obesity prevention effect and ingestion. Such animal meat proteins include cattle, pigs, sheep, rabbits, kangaroos and other meats and beef, and by-products and processed products thereof; chickens, turkeys, quail, etc., and by-products and houses. Articles: Fish meat such as fish and white fish, and by-products and processed products thereof; lettering of the above raw materials such as meat meal, meat bones, chicken meal, fish meat, and the like. Among these, chicken and fish are particularly preferable from the viewpoint of preventing obesity. In the case of using a mixture of a plurality of meat proteins, it is preferable to contain 30 to 100%, particularly 50 to 100%, of chicken and / or fish meat in the meat.
As the vegetable protein, soybean protein, wheat protein, wheat gluten, corn gluten and the like are preferable.

  In the pet food of the present invention, the animal or vegetable protein is preferably contained in an amount of from 5 to 70%, more preferably from 10 to 60%, and especially from 15 to 40% in terms of loss on drying.

  The pet food of the present invention may further contain a plant sterol. The plant sterol is preferably contained in the pet food in an amount of 0.1% or more, more preferably 0.5% or more, from the viewpoint of the cholesterol lowering effect. Moreover, the upper limit of plant sterol content should just be the range of 0.1 to 30%. Examples of plant sterols include free forms such as α-sitosterol, β-sitosterol, stigmasterol, campesterol, α-sitostanol, β-sitostanol, stigmasteranol, campestanol, and cycloartenol, and the like. And fatty acid esters, ferulic acid esters, cinnamic acid esters and the like.

  The pet food of the present invention can further contain bran, potatoes, vegetables, vitamins and the like. As rice bran, rice bran, bran or the like is used, and as rice cake, soybean meal is used. Examples of vegetables include vegetable extracts. Examples of vitamins include A, B1, B2, D, E, niacin, pantothenic acid, carotic acid and the like, preferably 0.05 to 10%. In addition, a gelling agent, a shape-retaining agent, a pH adjuster, a seasoning, an antiseptic, a nutrition reinforcing agent and the like that are generally used in pet foods can also be contained.

  Next, a method for manufacturing a pet food according to the present invention (hereinafter also simply referred to as “the present manufacturing method”) will be described. In this production method, an unsaturated fatty acid containing two or more double bonds is a mineral containing a fat and oil and a mineral component selected from iron, an iron compound, copper, and a copper compound that are 20% by mass or more of the fatty acid constituting the fat and oil. A method for producing a pet food containing 0.0005 to 1% by mass in terms of total conversion, characterized in that citric acid and an alkali metal salt of citric acid are combined in a total amount of 0.7% by mass or more in terms of citric acid. It is a manufacturing method of pet food.

  In the production method of the present invention, citric acid and alkali metal citrate are mixed at a mass ratio of citric acid / alkali metal salt = 1/10 to 10/1, particularly 1/5 to 5/1. Is preferred. The timing of adding citric acid and the alkali metal salt of citric acid can be appropriately selected, and specific procedures include the following procedures. That is, citric acid and an alkali metal salt of citric acid are mixed together with grains, meat meal, and mineral components such as iron and copper, and after sufficient mixing, extrusion is performed by an extruder while water is added with water or steam. Thereafter, it is dried with hot air (until the water content becomes 10% or less). It is desirable to code fats and oils whose double bonds are rich in two or more fatty acids after drying with hot air.

  The pet food of the present invention may be in any form such as a dry type, a wet type, a semi-moist type, a jerky type, a biscuit type, a gum type, a granular shape, a powdery shape, or a soup shape.

<Preparation of sample>
What dissolved the reagent used as a chelating agent shown below in a small amount of water was added to 3 g of high fat and oil containing diacylglycerol having the fatty acid composition of Table 1 and the glyceride composition of Table 2.
Each chelating agent reagent used and its concentration in the evaluation sample are as follows.
(Metal ion source)
Ferrous sulfate: Reagent, manufactured by Wako Pure Chemical, 1000ppm
Copper sulfate / copper chloride: Reagent, manufactured by Wako Pure Chemical Industries, Ltd. 1000 ppm
(Reagents used for chelating agents of iron and copper compounds)
Citric acid: Reagent, 1000 ppm manufactured by Wako Pure Chemical Industries
Sodium citrate (dihydrate): Reagent, manufactured by Wako Pure Chemical Industries, Ltd. 1000 ppm
Sodium gluconate: Reagent, manufactured by Wako Pure Chemical, 1000ppm
Sodium polyphosphate: Reagent, manufactured by Wako Pure Chemical Industries, Ltd. 1000 ppm
(Reagent used for chelating agent of iron compound)
Malic acid: Reagent, Wako Pure Chemicals 1000ppm
Sodium lactate: Reagent, manufactured by Wako Pure Chemical, 1000ppm
Sodium sulfate: Reagent, manufactured by Wako Pure Chemical, 1000ppm
Sodium metaphosphate: Reagent, manufactured by Wako Pure Chemical Industries, Ltd. 1000 ppm
(Reagent used for chelate evaluation of copper compounds)
Ascorbic acid: Reagent, 100 ppm manufactured by Wako Pure Chemical Industries
L-ascorbyl palmitate (VCP): 100 ppm made by DSM Nutrition

<Evaluation methods for various chelating agents>
The prepared sample is attached to an automatic oil and fat stability test apparatus (“Rancimat 743”, manufactured by Metrohm), kept at 120 ° C. while bubbling air under the condition of 20 mL / min, and the electrical conductivity is measured. The electric conductivity with respect to time is plotted, the rise time is obtained as shown in FIG. 1, and the stability is evaluated. This is an evaluation based on the principle that electrical conductivity increases due to oxidation, and a long rise time means high resistance to oxidation.

Test Example Tables 3 and 4 show the results of evaluating the effect of suppressing the oxidation promoting action of iron compounds and copper compounds on fats and oils with various chelating agents.
From these results, it was found that the oxidation promotion action of iron compounds and copper compounds on fats and oils was most suppressed by citric acid and sodium citrate.

Next, Table 5 shows the results of examining the influence of the iron compound and the copper compound on the suppression effect of the oxidation promoting action on the oil and fat by changing the salt state of citric acid. Also. The pH at that time was indicated.
The suppression effect at that time is ◎ when the rise time is 2 hours or more, ◯ if 1.5 hours or more and less than 2 hours, ◯ if 1 hour or more and less than 1.5 hours, △ if less than 1 hour × It was expressed.
From these results, it can be seen that the lower the pH, the lower the suppression effect.

<Preparation of sample>
A columnar pet food (kibble) having a diameter of 10 mm and a length of about 10 mm was manufactured by using the raw materials having the composition shown in Table 6 and adding the components shown in Table 9 and mixing extrusion molding with an extruder. .

  Table 7 shows the fatty acid composition of the fat extracted from the pet food, and Table 8 shows the glyceride composition of the fat extracted from the pet food.

  The content of unsaturated fatty acids containing two or more double bonds in the total fat / oil was 41.9% by mass, and the diacylglycerol content in the total fat / oil was 46.5% by mass. The total fat and oil contained in the pet food is 11.5% by mass, the content of unsaturated fatty acids containing two or more double bonds in the pet food is 4.8% by mass, and the diacylglycerol content in the pet food is 5. It was 3 mass%.

(Comparative Examples 1-3)
The same evaluation was performed on the pet food prepared in the same manner as in Example 1 except that the amounts of citric acid and sodium citrate were changed as shown in Table 9. In Comparative Example 3, 2.0% by mass of sodium polyphosphate was added for comparison.

<Evaluation method of odor of fats and oils>
It was stored for 16 weeks in an environment at a temperature of 40 ° C. and a relative humidity of 75%. The number of people who answered “I hardly feel a deodorized odor” was expressed as “4 or more”, “2 to 3” as Δ, and “1 or less” as “X”.

<Stability test method>
It was stored in an environment of a temperature of 40 ° C. and a relative humidity of 75%, and was taken out every 0, 4, 8, 12, and 16 weeks, and the POV (peroxide value) of the fats and oils contained was measured. The results are shown in Table 9.

<Measurement method of POV (peroxide value)>
The peroxide value (POV) was analyzed in accordance with 2.4.12.2-94 “Peroxide Value” of “Standard Oil and Fat Analytical Test Method” established by the Japan Oil and Fat Chemical Society.
(1) Extraction of fats and oils from pet food 50 g of kibble was pulverized, passed through a 1 mm sieve, the sample was filled into a cylindrical filter paper, and set in an open column with a cock. With the cock closed, 150 mL of diethyl ether was dropped from above with a dropping funnel, and after completion of dropping, the column cock was opened to collect diethyl ether. Next, the same operation was performed with 50 mL diethyl ether. The recovered diethyl ether was removed by topping with an evaporator to obtain an extracted fat from pet food.

(2) POV measurement of fats and oils 0.5 g of fats and oils extracted from pet food were weighed into a 250 ml tall beaker, and 20 ml of a solution of acetic acid / isooctane = 3/2 (volume ratio) was dissolved. 0.1 mL of saturated potassium iodide solution was added to the tall beaker and shaken well for 1 minute. Thereafter, 30 mL of distilled water was added to obtain a titration sample. The titration sample was titrated with 0.01N sodium thiosulfate. At this time, a POV automatic titrator, DMP Titorino 785 type, manufactured by Metrohm Shibata Co., Ltd. was used.

<Method of measuring citric acid equivalent value>
Add 5 mL of 5% perchloric acid to kibble (3.0 g) crushed with a mixer and shake for 10 minutes. After shaking, the volume is adjusted with a 50 mL volumetric flask. Ultrasonic treatment is performed for 10 minutes, and measurement is performed by a high performance liquid chromatograph (HPLC) method using the filtered solution. The HPLC measurement conditions are as follows.
Column: 2 Shodex Rspak KC-811, diameter 8mm x length 300mm (made by Showa Denko KK)
Column temperature: 40 ° C
Mobile phase: 3 mmol / L perchloric acid aqueous solution Reaction solution: 0.2 mmol / L bromothymol blue-containing 15 mmol / L disodium hydrogen phosphate aqueous solution Flow rate: mobile phase 1.0 mL / min, reaction solution 1.4 mL / min Measurement wavelength : 445 nm

  When the citric acid and the alkali metal salt of citric acid are blended in an amount of 0.7% by mass or more, the content of citric acid or a salt thereof as in Patent Documents 1 and 2 is less than 0.5% (Comparative Example 1). And the stability was remarkably improved compared with 2).

It is a figure which shows the time-dependent change of the electrical conductivity of a sample.

Claims (6)

The following components (A), (B) and (C),
(A) 6-50 mass% of fats and oils whose unsaturated fatty acid containing two or more double bonds is 20 mass% or more in the fatty acid which comprises fats and oils,
(B) Mineral component selected from iron, iron compound, copper and copper compound 0.0005 to 1% by mass in terms of mineral,
(C) Citric acid and / or salt thereof Pet food containing 0.7% by mass or more in terms of citric acid and having a pH of 3.5 to 7.0.   The pet food according to claim 1, wherein the salt of citric acid is an alkali metal citrate.   The pet food according to claim 1 or 2, wherein the component (C) contains both citric acid and a salt of citric acid.   The pet food according to any one of claims 1 to 3, wherein 10 to 85% by mass of diacylglycerol is contained in the oil (A).   An oil and fat whose unsaturated fatty acid containing two or more double bonds is 20% by mass or more of the fatty acid constituting the oil and fat, and a mineral component selected from iron, iron compound, copper and copper compound is 0.7. A method for producing a pet food comprising 0005 to 1% by mass, wherein citric acid and alkali metal salts of citric acid are combined in a total amount of 0.7% by mass or more in terms of citric acid. .   The citric acid and the alkali metal citrate salt are added so that the citric acid / alkali metal citrate salt (mass ratio) in the pet food is 10/1 to 1/10. Production method.

Images