JP2010094506A - Deodorant and method of manufacturing deodorant - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a deodorant which has high deodorizing effect and can be used for a vital body, and to provide a method of manufacturing the deodorant. <P>SOLUTION: The deodorant contains a water soluble extract of vetiver plant. The extract solution containing the water soluble extract of vetiver plant can be used for a deodorant as it is, and also can be used for the deodorant by concentrating the extract solution by a method such as centrifugal concentration, heating concentration, vacuum concentration, and autoclave concentration to be used as the deodorant. The extract solution including the water soluble extract of vetiver plant is freeze-dried and sieved, and then micropowder under 250 micrometer meshes (60 mesh) also can be used as the deodorant. <P>COPYRIGHT: (C)2010,JPO&INPIT

Description

  The present invention relates to a deodorant and a method for producing the deodorant. Specifically, the present invention relates to a deodorant using a highly safe natural material and a method for producing the deodorant.

In recent years, the number of houses with high airtightness has increased, and the resident's desire for cleanliness has increased, and there has been a growing demand for deodorizing pet odors such as dogs and cats and daily odors.
In hospitals and elderly care facilities, there is an increasing demand to deodorize the unpleasant urine odor (ammonia odor) associated with urinary incontinence by patients and the elderly.

Various synthetic compounds are known as active ingredients of a deodorant having a high deodorizing effect, and many deodorizers using these compounds have been proposed.
For example, Patent Document 1 describes a deodorant comprising an aqueous solution containing 10 to 1000 ppm of a chlorine-containing oxidizing agent and adjusted to pH 8 to 13 with an inorganic salt or an organic salt.
Patent Document 2 describes a deodorant containing a jasmonic acid compound and a delta lactone as a deodorizing component.
Patent Document 3 describes a deodorant composed of an aqueous solution containing a halide salt of a 3d transition metal.

JP 2001-316213 A JP 2002-253651-A JP 2002-85537 A

  However, the compounds used as active ingredients of these conventional deodorants have high odor resolution, but are safe when used on living things such as pets and human bodies directly, or on pets and human bodies. Still had problems.

  The present invention was devised in view of the above points, and has a high deodorizing effect and can be used safely with respect to a living body, and a deodorant for producing such a deodorant. It aims at providing the manufacturing method of.

  In order to achieve the above object, the deodorant of the present invention contains an extract of a vetiver plant.

  In the deodorant of the present invention, the vetiver plant is preferably a vetiver root.

  In the deodorizer of the present invention, the extract preferably contains a water-soluble extract.

  Here, the deodorant can be made liquid by the water-soluble extract.

Moreover, the deodorizer of this invention can be sprayed as a deodorizing liquid, when it is a liquid of a water-soluble extract.
The liquid may be an aqueous solution or a colloidal dispersion.

  Moreover, in the deodorizer of this invention, when an extract is a dry powder and a dry powder is a magnitude | size of 250 micrometers or less, a higher ammonia deodorizing effect is acquired and it is preferable.

  In the deodorizer of the present invention, the extract preferably contains an organic solvent extract.

Moreover, in order to achieve said objective, the manufacturing method of the deodorizer of this invention has an extraction process which extracts a water-soluble extract from a vetiver plant.
The vetiver plant is preferably a vetiver root.

  Moreover, in the manufacturing method of the deodorizer of this invention, it is preferable to have a drying process which dries the water-soluble extract of a vetiver plant or a vetiver root after an extraction process.

  Moreover, in the manufacturing method of the deodorizer of this invention, it is preferable that a drying process freezes and dries the water-soluble extract of a vetiver plant or a vetiver root.

  In addition, in the method for producing a deodorant of the present invention, when a drying step is followed by a refining step for refining the dried powder obtained by the drying step to a size of 250 μm or less, a higher ammonia deodorizing effect is obtained. The deodorant which has can be manufactured.

  Moreover, the manufacturing method of the deodorizer of this invention has an extraction process which extracts an organic-solvent extract from a vetiver plant.

The deodorizer according to the present invention has a high deodorizing effect and can be safely used for living bodies.
With the method for producing a deodorant according to the present invention, it is possible to produce a deodorant that has a high deodorizing effect and can be safely used for a living body.

It is a graph which shows the ammonia deodorizing effect of the extract of a vetiver root. It is a graph which shows the ammonia deodorizing effect of the fine powder extract of a vetiver root. It is the graph which compared the ammonia deodorizing effect of the extract of a vetiver root, and a commercially available natural raw material type deodorizer. It is a graph which shows the ammonia deodorizing effect of the extract of the vetiver root from which the brown component was removed. It is a graph which shows the ammonia deodorization test result of the extract each extracted with ethanol, methanol, chloroform, and isopropanol. It is a graph which shows the ammonia deodorizing test result of the extract each extracted with n-hexane, methanol, water, and ethyl acetate. It is a graph which shows the ammonia deodorization test result of the extract extracted with ethyl acetate, methanol, ethanol, and water, respectively. It is a graph of the ammonia removal amount of various extracts 40 minutes after contact with ammonia gas and an extract. It is a graph of the amount of ammonia removal per 1 mg of various extracts after 40 minutes from the contact between ammonia gas and the extract. It is a graph which shows the solid substance weight of each fraction. It is a graph which shows the ammonia removal amount of each fraction. It is a graph which shows the ammonia removal amount of each fraction 30 minutes after contact with ammonia gas and a fraction. It is a graph of the ammonia removal amount per 1 mg of the extract of each fraction 30 minutes after contact of ammonia gas and the fraction.

  The deodorant to which the present invention is applied includes a water-soluble extract of a vetiver plant. Vetiver is a perennial plant of the grass family native to Indonesia.

  Moreover, any extraction method may be used as long as a water-soluble extract can be extracted from a vetiver plant, for example, a method of immersing a dried vetiver plant in water and performing autoclave extraction at 100 to 125 ° C. for 30 to 40 minutes, Examples include a method in which a dried vetiver plant is immersed in water and extracted with hot water at 50 to 65 ° C. for 1 to 2 hours.

In addition, an extract containing a water-soluble extract of a vetiver plant can be used as a deodorant as it is, but the extract is concentrated by a concentration method such as centrifugal concentration, heat concentration, reduced pressure concentration, autoclave concentration or the like. It can also be used as an odorant.
In addition, a concentrated extract containing a water-soluble extract of a vetiver plant is freeze-dried, and the freeze-dried powder is finely powder having an opening of 250 micrometers (60 mesh) or less and used as a deodorant. You can also.

  Moreover, the extract is exhibiting brown color by extracting from the vetiver plant. However, it is preferable to remove the brown component since the clothing will be discolored when the extract is colored when sprayed on the clothing. Therefore, for example, it is preferable to remove the brown component by filtering the extract with filter paper, filtering with a cellulose membrane having a membrane pore size of 0.2 μm, or adsorbing the brown component with a synthetic adsorbent.

Further, when the deodorant of the present invention containing a water-soluble extract of a vetiver plant is diluted in water to form an aqueous solution (deodorant liquid) and this deodorant liquid is put into a humidifier or the like, the odor of the room continues to disappear. In addition, by adding the deodorant of the present invention to the wash water, the fishy odor (trimethylamine), sweat odor (ammonia odor), pet odor and tobacco odor attached to the clothes are eliminated. Moreover, the deodorant for bathing which uses the deodorant of this invention in the hot water of a bathtub removes an aging odor and an ammonia odor, and also suppresses the unpleasant smell of hot water.
In addition, the deodorizing solution of the present invention can be expected to suppress the growth of bacteria such as Escherichia coli and staphylococci and make it difficult for insects such as cockroaches and mites to come close.

[Ammonia deodorization test 1]
100 g of dried vetiver root (an example of a vetiver plant) is soaked in 1.8 liters of water and subjected to autoclave extraction at 121 ° C. for 40 minutes, followed by centrifugal concentration and filtration to obtain an autoclave extract. It was. The extraction rate of water-soluble components in autoclave extraction (weight of water-soluble components per 100 g of vetiver root) was 6.31%. In addition, as a result of performing autoclave extraction similarly except the point which is not filtering, the extraction rate of the water-soluble component was 7.66%.
Moreover, 100 g of dried vetiver roots were immersed in 1.8 liters of water, and hot water extraction was performed at 55-60 ° C. for 1.5 hours, followed by centrifugal concentration and filtration to obtain a hot water extract. . The extraction rate of the water-soluble component in the hot water extraction was 6.42%.
Next, the cap at the mouth of the 5 liter Tedlar bag was removed and replaced with a silicon tube having a thickness suitable for the detection tube, and the corner of the Tedlar bag was bent as a reagent injection place and stopped with tape. A cut end was put on the unfolded end so that the volume did not change, and a hot water extract precisely weighed to about 5 ml was put through the cut end, and then the cut end was sealed with tape.
Then, the air inside the Tedlar bag was evacuated with a pump, 4 liters of compressed air was introduced using an integrating flow meter, and the silicone tube was closed with a clip and sealed. A microsyringe (10 microliter volume) was stabbed into a portion that was bent and stopped with tape, that is, a portion to which the hot water extract was not attached, and 3 microliters of 25% ammonia water was injected.
Then, the portion where the hole was opened by the microsyringe was immediately closed with tape, and the entire Tedlar bag was heated with a dryer to vaporize the solution (initial ammonia concentration: 600 ppm). Then, after 2 minutes, 10 minutes, 20 minutes, 40 minutes and 80 minutes, the ammonia concentration is measured by using a detector and a detector tube at intervals, and the residual ammonia rate is calculated. did.
Moreover, after putting the autoclave extract accurately weighed to about 5 ml from the cut end, it was sealed with a tape and the same test as above was performed.
For comparison, simple water precisely weighed to about 5 ml was added from the cut end, and the cut end was sealed with tape, and the same test as described above was performed. The results are shown in FIG.

  As can be seen from FIG. 1, both the autoclave extract and the hot water extract extracted from the dried vetiver roots are 50% or more when the deodorization of ammonia has started for 2 minutes (ie, ammonia remaining). The rate was 50% or less).

[Ammonia deodorization test 2]
200 g of dried vetiver roots were immersed in 1 liter of water, and hot water extraction was performed at 55 to 60 ° C. for 1.5 hours to obtain a hot water extract.
Next, the obtained hot water extract was heated and concentrated at 100 ° C. for 1.5 hours. The extraction rate of the water-soluble component was 2.53 ± 0.064%. After concentration by heating, the extract was freeze-dried to obtain an extract.
Further, the separately obtained hot water extract was concentrated under reduced pressure at 60 ° C. under a pressure of 100 mmHg. The extraction rate of the water-soluble component was 2.41 ± 0.233%. After concentration under reduced pressure, the extract was lyophilized to obtain an extract.
Moreover, the hot water extract obtained separately was autoclaved at 121 ° C. for 40 minutes. The extraction rate of the water-soluble component was 2.35 ± 0.099%. After concentrating in an autoclave, it was freeze-dried to obtain an extract.
Moreover, the hot water extract obtained separately was freeze-dried without concentrating at all (extraction rate of water-soluble component: 2.94 ± 0.486%) to obtain an extract.

And the extract obtained by freeze-drying after concentration by heating was adjusted to a fine powder having an opening of 250 micrometers (60 mesh) or less.
On the other hand, the hot water extract obtained by concentration under reduced pressure and freeze-drying was adjusted to a fine powder having an opening of 250 micrometers (60 mesh) or less.
Next, the cap at the mouth of the 5 liter Tedlar bag was removed and replaced with a silicon tube having a thickness suitable for the detection tube, and the corner of the Tedlar bag was bent as a reagent injection place and stopped with tape. A lyophilized extract concentrated under reduced pressure and adjusted to a fine powder with an opening of 250 micrometers (60 mesh) or less, which was cut into an unfolded end so that the volume did not change and was precisely weighed to about 0.4 g. After putting through the cut, the cut was sealed with tape.
Then, the air inside the Tedlar bag was evacuated with a pump, 4 liters of compressed air was introduced using an integrating flow meter, and the silicone tube was closed with a clip and sealed. Puncture a microsyringe (10 microliters) into the part that has been bent and secured with tape, that is, the part to which the vacuum concentrated lyophilized extract adjusted to a fine powder with an opening of 250 micrometers (60 mesh) or less is not attached. 3 microliters of 25% aqueous ammonia was injected.
Then, the portion where the hole was opened by the microsyringe was immediately closed with tape, and the entire tedlar bag was heated with a dryer to vaporize the solution (initial ammonia concentration: 420 ppm). Then, after 2 minutes, 10 minutes, 20 minutes, 40 minutes, and 80 minutes, the ammonia concentration is measured using a detector and a detector tube, and the ammonia removal rate is calculated. did.
In addition, a heat-concentrated freeze-dried extract adjusted to a fine powder with a mesh size of 250 micrometers (60 mesh) or less, precisely weighed to about 0.4 g, was put through the cut and sealed with a tape. The same test was conducted. The results are shown in FIG.

As can be seen from FIG. 2, the fine powder extract obtained by hot water extraction from the dried vetiver root and then heating and concentration is deodorized by 80% when 2 minutes have passed since the deodorization of ammonia was started. In addition, the fine powder extract obtained by hot water extraction from dried vetiver roots and then concentration under reduced pressure was deodorized 60% when 2 minutes had passed since deodorization of ammonia was started. .
Further, both the fine powder extract obtained by heating and concentration and the fine powder extract obtained by concentration under reduced pressure were deodorized by 90% or more when 10 minutes had passed since deodorization of ammonia was started.
Therefore, the hot water extract extracted from the vetiver root is extracted with hot water from the vetiver root, and then concentrated by heating to obtain a fine powder having an opening of 250 micrometers (60 mesh) or less. The one showed a higher ammonia deodorizing effect.

[Ammonia deodorization test 3]
100 g of dried vetiver root is immersed in 1.8 liters of water, and hot water extraction is performed at 55-60 ° C. for 1.5 hours, followed by centrifugal concentration and filtration, and further at 100 ° C. for 1.5 hours. Concentration by heating gave a vetiver root hot water extract. The extraction rate of the water-soluble component was 5.39%.
In addition, in order to compare with vetiver root hot water extract, corn-derived component extract deodorant [Fabries (registered trademark), manufactured by P & G Co.] (water-soluble component content: 1.94 g / 100 ml) and catechin-derived A component extract liquid deodorant [Risesh (registered trademark), manufactured by Kao Corporation] (water-soluble component content: 1.11 g / 100 ml) was prepared.
Next, the cap at the mouth of the 5 liter Tedlar bag was removed and replaced with a silicon tube having a thickness suitable for the detection tube, and the corner of the Tedlar bag was bent as a reagent injection place and stopped with tape. A cut end was put in the unfolded end so that the volume did not change, and a vetiver root hot water extract precisely weighed to about 3 ml was put through the cut end, and then the cut end was sealed with tape.
Then, the air inside the Tedlar bag was evacuated with a pump, 4 liters of compressed air was introduced using an integrating flow meter, and the silicone tube was closed with a clip and sealed. A microsyringe (10 microliter volume) was stabbed into a portion that was bent and stopped with tape, that is, a portion where the vetiver root hot water extract was not attached, and 3 microliters of 25% ammonia water was injected.
Then, the portion where the hole was opened by the microsyringe was immediately closed with tape, and the entire Tedlar bag was heated with a dryer to vaporize the solution (initial ammonia concentration: 400 ppm). Then, after 2 minutes, 10 minutes, 20 minutes, and 40 minutes, the ammonia concentration was measured using a detector and a detector tube, and the residual ammonia rate was calculated.
Moreover, after putting the corn-derived component extract deodorizer precisely weighed to about 3 ml from the cut, it was sealed with a tape and the same test as above was performed.
Further, a catechin-derived component extract deodorant precisely weighed to about 3 ml was added from the cut end, and the cut end was sealed with tape, and the same test as described above was performed.
In addition, a simple water precisely weighed to about 3 ml was added from the cut end, and then the cut end was sealed with tape, and the same test as described above was performed. The results are shown in FIG.

As can be seen from FIG. 3, the vetiver root hot water extract was deodorized 80% (that is, ammonia remaining rate 20%) after 10 minutes from the start of deodorization of ammonia. The extract liquid deodorant only deodorized 65% (that is, the ammonia residual ratio was 35%) when 10 minutes had passed since the deodorization of ammonia was started. In addition, the catechin-derived component extract deodorizer only deodorized 40% (that is, the ammonia remaining rate was 60%) when 10 minutes had passed since deodorization of ammonia was started.
Furthermore, the vetiver root hot water extract decreased the residual ammonia rate after 20 minutes or 40 minutes after the start of deodorization of ammonia, whereas the corn-derived component extract deodorant was ammonia. After 10 minutes from the start of deodorization, there was almost no change in the residual ammonia rate, and in the case of catechin-derived component extract liquid deodorizer, the residual ammonia rate was almost 2 minutes after the start of deodorization of ammonia. There was no change.

[Ammonia deodorization test 4]
100 g of dried vetiver roots were soaked in 1.8 liters of water and subjected to hot water extraction at 55 to 60 ° C. for 1.5 hours, and then the obtained hot water extract was filtered through filter paper, and further membrane The mixture was filtered through a cellulose membrane having a pore size of 0.2 μm to obtain a first vetiver root hot water extract from which the brown component was removed.
Further, after immersing 100 g of dried vetiver roots in 1.8 liters of water and performing hot water extraction at 55-60 ° C. for 1.5 hours, the obtained hot water extract was filtered through a filter paper, Further filtered through a cellulose membrane having a membrane pore size of 0.2 μm, and then the brown component was removed by further adsorbing the brown component with a synthetic adsorbent [Diaion (registered trademark) HP20, manufactured by Mitsubishi Chemical Corporation]. A vetiver root hot water extract was obtained.
Further, after immersing 100 g of dried vetiver roots in 1.8 liters of water and performing hot water extraction at 55-60 ° C. for 1.5 hours, the obtained hot water extract was filtered through a filter paper, Further filtered through a cellulose membrane having a membrane pore size of 0.2 μm, and then a third vetiver from which the brown component was removed by further adsorbing the brown component with a synthetic adsorbent [Separbeads (registered trademark) SP207, manufactured by Mitsubishi Chemical Corporation]. A root hot water extract was obtained.
Next, the cap at the mouth of the 5 liter Tedlar bag was removed and replaced with a silicon tube having a thickness suitable for the detection tube, and the corner of the Tedlar bag was bent as a reagent injection place and stopped with tape. A cut was made at the unfolded end so that the volume did not change, and the first vetiver root hot water extract precisely weighed to about 3 ml was put through the cut and then sealed with tape.
Then, the air inside the Tedlar bag was evacuated with a pump, 4 liters of compressed air was introduced using an integrating flow meter, and the silicone tube was closed with a clip and sealed. A microsyringe (10 microliter volume) was pierced into the portion that was bent and stopped with tape, that is, the portion where the first vetiver root hot water extract was not adhered, and 3 microliters of 25% ammonia water was injected.
Then, the portion where the hole was opened by the microsyringe was immediately closed with tape, and the entire Tedlar bag was heated with a dryer to vaporize the solution (initial ammonia concentration: 400 ppm). Then, after 2 minutes, 10 minutes, 20 minutes, and 40 minutes, the ammonia concentration was measured using a detector and a detector tube, and the residual ammonia rate was calculated.
Moreover, after putting the 2nd vetiver root hot water extract precisely weighed to about 3 ml from a cut end, it sealed the cut end with the tape, and performed the same test as the above.
Further, a third vetiver root hot water extract precisely weighed to about 3 ml was put through the cut end, and then the cut end was sealed with tape, and the same test as described above was performed.
In addition, a simple water precisely weighed to about 3 ml was added from the cut end, and then the cut end was sealed with tape, and the same test as described above was performed. The results are shown in FIG.

  As can be seen from the comparison between FIG. 3 and FIG. 4, the vetiver root hot water extract is more effective in deodorizing ammonia than the catechin-derived component extract deodorant even when the brown component is removed by the cellulose membrane or the synthetic adsorbent. it was high.

[Extraction rate of various extracts]
1 g of vetiver root pulverized product obtained by pulverizing the dried vetiver roots with a Willet pulverizer was immersed in 10 ml of pure water, followed by autoclave extraction at 121 ° C. for 40 minutes, followed by filtration. The filtrate was concentrated by centrifugation. Next, the residue obtained by filtration was immersed in 10 ml of pure water, followed by autoclave extraction at 121 ° C. for 40 minutes, followed by filtration. The filtrate was concentrated by centrifugation. And the autoclave extraction was similarly performed with respect to the obtained residue, the residue was obtained, and the filtrate was concentrated by centrifugation. That is, extraction was performed three times in total.
And extraction rate (%) was computed with the solid substance weight extracted with respect to the weight of the vetiver root used for extraction. The results are shown in Table 1 together with extraction conditions such as solvent ratio.
On the other hand, autoclave extraction was similarly performed using “pure water and ethanol 10%” or “pure water and ethanol 20%” instead of pure water as a solvent used for extraction.

Further, 1 g of a 1 cm long vetiver root obtained by cutting the dried vetiver root into 1 cm length with a cutting shear was immersed in 18 ml of n-hexane, and subjected to extraction by shaking at 20 ° C. for 24 hours. Then, it filtered. The filtrate was concentrated by centrifugation. Next, the residue obtained by filtration was soaked in 18 ml of n-hexane, and subjected to extraction with shaking at 150 rpm for 24 hours, followed by filtration. The filtrate was concentrated by centrifugation. And extraction rate (%) was computed with the solid substance weight extracted with respect to the weight of the vetiver root used for extraction. The results are shown in Table 1 together with extraction conditions such as solvent ratio.
On the other hand, as an organic solvent used for extraction, instead of n-hexane, methanol, ethanol, ethyl acetate, chloroform, and isopropanol (2-propanol) were used for extraction with shaking. When methanol was used as a solvent, 1 g of vetiver root pulverized product obtained by pulverizing dried vetiver roots with a Willet pulverizer was also subjected to shaking extraction.

Judging from Table 1, regarding the extraction method, the extraction rate of the components contained in the vetiver root is considered to depend on the solvent used for elution rather than the number of extractions and the ratio of the solvent to the components. However, the extraction method that heats or pressurizes with pure water (autoclave) has the highest recovery rate, so the compound extracted by applying heat or pressure is contained only in the autoclaved pure water extract. It seems to be.
In addition, when compared only with the extraction method using an organic solvent, the methanol extract showed the highest extraction rate. In addition, no significant difference was observed in the extraction rate when using either a vetiver root pulverized product or a 1 cm long vetiver root. Therefore, it is considered that either crushed material or 1 cm length may be used as the shape of the vetiver root.
In addition, regarding the color of the extract, it was found that the organic solvent extract had a pale yellow, reddish brown or brown color. Moreover, the extract extracted by adding 10 to 20% ethanol to pure water or pure water exhibited a pale yellow color. That is, it is considered that the brown pigment component is more extracted when extracted using an organic solvent.

[Ammonia deodorization test 5]
1 ml of an extract (extract concentration: 20 mg / ml) extracted from various roots of vetiver roots was added to the filter paper, and the solvent was completely removed with a vacuum desiccator for about 24 hours.
Next, the filter paper to which the extract is added is brought into contact with ammonia gas in a 1 liter Tedlar bag, and the concentration in the system after 0 minutes, 0.5 minutes, 10 minutes, 20 minutes and 40 minutes is determined. It was measured. The measurement was performed using a glass detector tube (manufactured by Gastec).
In addition, as a control for comparison, a filter paper to which only the solvent is added is prepared, and after removing the solvent completely with a vacuum desiccator for about 24 hours, the filter paper (no added filter paper) and ammonia gas are placed in a 1 liter Tedlar bag. The concentration in the system was measured after 0 minute, 0.5 minute, 10 minutes, 20 minutes and 40 minutes.
Moreover, the solvent used is ethanol, methanol, chloroform, isopropanol, n-hexane, water, and ethyl acetate.

  The results are shown in FIGS. 5 (a), 6 (a) and 7 (a) are graphs showing the relationship between the ammonia concentration and the elapsed time, and FIG. 5 (b), FIG. 6 (b) and FIG. (B) is the graph which showed the relationship between ammonia residual rate and elapsed time. Here, the ammonia residual ratio was calculated with a blank (non-added filter paper) as 100.

  FIG. 8 shows a graph of the amount of ammonia removed from various extracts after 40 minutes from the contact between the ammonia gas and the extract. FIG. 9 shows various extractions after 40 minutes from the contact between the ammonia gas and the extract. The graph of the amount of ammonia removal per 1 mg of a thing is shown.

As can be seen from FIG. 5 to FIG. 9, as a result of comparing the ammonia deodorizing effect between the various extracts, it was found that the methanol extract showed the highest effect.
In addition, an ammonia deodorizing effect was low in an extract of a relatively low-polarity solvent such as n-hexane or ethyl acetate. From the results of these deodorizing effects and the properties of the extract shown above, as a substance having an ammonia deodorizing effect contained in the vetiver root, it is a component with relatively high hydrophilicity by heating or pressurizing treatment. It is considered that it has some chemical characteristics such as a non-denaturing and brown pigment component.
In addition, as a method for more efficiently extracting the active ingredient for ammonia contained in the vetiver root, if extraction is performed with an organic solvent, it is more likely to use a relatively polar solvent such as methanol, isopropanol, or ethanol. If the active ingredient can be extracted and extracted with pure water, it is considered that more active ingredients can be extracted by using autoclave extraction.

[Deodorizing active fraction by silica gel column fractionation]
The methanol extract of vetiver root was fractionated by silica gel column chromatography. Further, 200.191 g of ground vetiver roots were extracted twice with 1800 ml of methanol, and then the two extracts were mixed, concentrated and dried with an evaporator to obtain a sample.
Further, using this sample, as shown in Table 2, the elution was carried out by changing the polarity of the developing solvent sequentially from n-hexane 100% / ethyl acetate 0% to methanol 100% / ethyl acetate 0% for a total of 81 The fraction (extracted fraction) was fractionated (extract recovery rate 93.20%).
The silica gel column chromatography fractionation conditions are as follows.
That is, Wakogel C-200 (trade name, manufactured by Wako Pure Chemical Industries, Ltd.) (75-150 μm) was used as the silica gel, the methanol extract 20.265 g, the amount of adsorbed silica gel 60.027 g, the column diameter 10.5 cm, the column length The length was 97 cm, the developed width was 35.5 cm, the column volume was 30.7 L, and the amount of silica gel was 1600.20 g.

Next, 500-1000 ml was fractionated per fraction.
Then, after fractionation, the mixture was immediately concentrated by an evaporator to dryness, and the weight of solid matter was measured. The solid weight of each fraction is shown in FIG.
As can be seen from FIG. 10, it was inferred that a particularly large amount of compound was contained from the 12th fraction to the 14th fraction or from the 61st fraction to the 69th fraction.

Next, a solution obtained by dissolving 2 mg of solids in each fraction with 10 μl of solvent was added to the filter paper, and the solvent was completely removed with a vacuum desiccator for about 24 hours. Then, the filter paper and ammonia gas were brought into contact in an Erlenmeyer flask having a capacity of 100 ml. The amount of ammonia removed from each fraction is shown in FIG. In FIG. 11, “MeOH” means methanol.
Moreover, the ammonia removal amount was calculated from the ammonia concentration in the system of the non-added filter paper and the sample-added filter paper. Epicatechin gallate (ECg) and catechin (C) were used for comparison because they have been reported to be effective in deodorizing ammonia.
In addition, the used Erlenmeyer flask was attached with a rubber stopper having a hole in its mouth, and a glass tube having a diameter of 5 mm and a length of 10 cm was inserted into the hole so as to penetrate inside and outside of the Erlenmeyer flask. . At the outside of the glass tube (the part that protrudes out of the Erlenmeyer flask), attach a rubber tube with a length of 15 cm, fold the rubber tube from the half and hold it with a clip so that the gas in the Erlenmeyer flask does not leak out. I made it.

As can be seen from FIG. 11, from the results of comprehensive screening, the 17th fraction, the 26th to 28th fraction, the 41st to 43rd fraction, the 60th to 64th fraction, the 68th fraction, the 69th fraction and the 74th to 81st fraction. The ammonia concentration in the system was reduced by 20 ppm or more than the additive-free filter paper, and it was estimated that a component having an ammonia deodorizing effect was contained.
Therefore, using a methanol extract as a comparative control, among these fractions, the 17th fraction, the 27th fraction, the 41st fraction, the 63rd fraction, the 69th fraction and the 78th fraction were used using a 1 liter Tedlar bag. An ammonia deodorization test was conducted. The results are shown in FIGS. FIG. 12 is a graph showing the amount of ammonia removed from each fraction 30 minutes after the contact between the ammonia gas and the fraction, and FIG. 13 shows the amount per 1 mg of the extract of each fraction 30 minutes after the contact between the ammonia gas and the fraction. The graph of ammonia removal amount is shown.

As can be seen from FIGS. 12 and 13, it was found that the 17th and 41st fractions exhibited the same deodorizing effect as the methanol extract. Moreover, the adsorption amount per 1 mg of extract decreased compared with the experimental result (FIG. 9) which examined the ammonia deodorizing effect between various solvent extracts.
However, the fractions used were obtained by fractionation using a methanol extract, and the effects of these fractions have been studied. The 17th, 27th, 41st and 63rd fractions are particularly ammonia. It is suggested that it contains an effective ingredient.
When these results are considered together with the fractionation conditions, the active ingredient for ammonia contained in the vetiver root methanol extract has different chemical characteristics and is dispersed in several fractions. Is guessed.

  As described above, the extract containing the water-soluble extract extracted from the roots of vetiver (vetiver root hot water extract) is 50% or more after 2 minutes from the start of deodorization of ammonia. It has a high deodorizing effect that it can be deodorized, and since it is an extract extracted from the roots of vetiver, which is a natural material, it can be safely used for living organisms.

  Further, the vetiver root hot water extract shows a higher ammonia deodorizing effect as time passes than commercially available corn-derived component extract deodorants and catechin-derived component extract deodorants.

  In addition, although the extract has a brown color due to extraction from the roots of vetiver, it is preferable to remove the brown component because the color of the extract will change when sprayed on the clothing. However, even if the brown component is removed, the vetiver root hot water extract has a higher ammonia deodorizing effect than the catechin-derived component extract deodorant.

  Further, since the vetiver root hot water extract is an aqueous solution, it can be sprayed, and thus can be used very easily and has a wide range of applications. In addition, since it is a deodorant composed only of a natural material called vetiver root hot water extract, there is no worry about stickiness even when sprayed.

  In addition, a methanol extract obtained by organic solvent extraction, particularly methanol extraction from vetiver root, has a larger amount of ammonia removal than a water-soluble extract obtained by water extraction, and thus has a high ammonia deodorizing effect.

Claims (15)

Deodorant containing vetiver plant extract. The deodorizer according to claim 1, wherein the vetiver plant is a vetiver root. The deodorizer according to claim 1 or 2, wherein the extract contains a water-soluble extract. The deodorizer according to claim 3, which is a liquid of the water-soluble extract. The deodorizer according to claim 4, wherein the liquid is an aqueous solution. The deodorizer according to claim 4, wherein the liquid is a colloidal dispersion. The deodorizer according to any one of claims 1 to 3, wherein the extract is a dry powder. The deodorizer according to claim 7, wherein the dry powder has a size of 250 μm or less. The deodorizer according to claim 1 or 2, wherein the extract contains an organic solvent extract. A method for producing a deodorant comprising an extraction step of extracting a water-soluble extract from a vetiver plant. The method for producing a deodorant according to claim 10, wherein the vetiver plant is a vetiver root. The method for producing a deodorant according to claim 10, further comprising a drying step of drying the water-soluble extract after the extraction step. The method for producing a deodorant according to claim 12, wherein in the drying step, the water-soluble extract is frozen and dried. The method for producing a deodorant according to claim 12, further comprising a refining step for refining the dry powder obtained by the drying step to a size of 250 μm or less after the drying step. A method for producing a deodorant comprising an extraction step of extracting an organic solvent extract from a vetiver plant.

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