JP2010088349A - セルラーゼ測定試薬およびセルラーゼの測定方法 - Google Patents
セルラーゼ測定試薬およびセルラーゼの測定方法 Download PDFInfo
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- JP2010088349A JP2010088349A JP2008261575A JP2008261575A JP2010088349A JP 2010088349 A JP2010088349 A JP 2010088349A JP 2008261575 A JP2008261575 A JP 2008261575A JP 2008261575 A JP2008261575 A JP 2008261575A JP 2010088349 A JP2010088349 A JP 2010088349A
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- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 22
- 229920002678 cellulose Polymers 0.000 claims abstract description 49
- 239000001913 cellulose Substances 0.000 claims abstract description 49
- 150000004696 coordination complex Chemical class 0.000 claims abstract description 26
- 229910052751 metal Inorganic materials 0.000 claims abstract description 25
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- 239000008103 glucose Substances 0.000 claims abstract description 9
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- 239000000413 hydrolysate Substances 0.000 abstract 1
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- 229920000742 Cotton Polymers 0.000 description 7
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- XCJYREBRNVKWGJ-UHFFFAOYSA-N copper(II) phthalocyanine Chemical compound [Cu+2].C12=CC=CC=C2C(N=C2[N-]C(C3=CC=CC=C32)=N2)=NC1=NC([C]1C=CC=CC1=1)=NC=1N=C1[C]3C=CC=CC3=C2[N-]1 XCJYREBRNVKWGJ-UHFFFAOYSA-N 0.000 description 3
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- 241000196324 Embryophyta Species 0.000 description 2
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Abstract
【解決手段】セルロースの構成グルコースの水酸基に反応基を介して金属錯体を結合した金属錯体結合型セルロースから成るセルラーゼ測定試薬、および、このセルラーゼ測定試薬にセルラーゼを作用させてセルロースのグリコシド結合を加水分解し、生成する加水分解物に固定された金属錯体中の金属の濃度に基づき、セルラーゼの濃度を測定する、セルラーゼの測定方法。
【選択図】なし
Description
(1)セルラーゼ測定試薬(金属錯体結合型セルロース)の調製:
炭酸ナトリウム:2.0g、硫酸ナトリウム:5.0g、C.I.Reactive Blue:21:0.6gを溶かした水溶液100mLに脱脂綿5.0gを添加し、攪拌しながら30℃で35分反応させた。その後、70℃にて60分間で反応させ、反応の後、ジメチルスルホキシド、メタノール-アンモニア(50:1v/v)混合溶液で洗浄し、乾燥させて金属錯体結合型セルロースを得た。この金属錯体結合型セルロースにおいて、金属錯体の割合は、金属錯体結合型セルロース1.0g当たり30μmolであり、セルロースのグルコース約600個当たり1個であった。
濃度の異なる複数のセルラーゼ水溶液を調製して試料とした。20mLサンプル管に各10mLを加え、恒温槽にて45℃の一定温度に保持した。その後、上記のセルラーゼ測定試薬0.02gを加え、1時間振とうした。振とうの後、上澄み溶液を分取し、フレームレス原子吸光光度計(日立ハイテクノロジー社製「 Z−2000型」)にて、試料溶液を20μL注入し、銅の分析を行った。
実施例1における銅の分析において、フレームレス原子吸光光度計に代えてICP発光分光分析装置(パーキンエルマー社製 「Optima5300DV型」)を使用した他は、実施例1と同様に行った。その結果、銅の分析値は、フレームレス原子吸光光度計を使用した実施例1と同様の値であった。なお、ICP発光分光分析装置における測定条件は次の通りである。すなわち、試料溶液と80ppbイットリウム内標準溶液(5%硝酸溶液)をオンライン内標準添加法にて流路内にて混合した。その際の吸引流速は0.25mL/minとした。
Claims (3)
- セルロースの構成グルコースの水酸基に反応基を介して金属錯体を結合した金属錯体結合型セルロースから成ることを特徴とするセルラーゼ測定試薬。
- 金属錯体が金属フタロシアニンである請求項1に記載のセルラーゼ測定試薬。
- 請求項1又は2に記載のセルラーゼ測定試薬にセルラーゼを作用させてセルロースのグリコシド結合を加水分解し、生成する加水分解物に固定された金属錯体中の金属の濃度に基づき、セルラーゼの濃度を測定することを特徴とするセルラーゼの測定方法。
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Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
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JPH11511789A (ja) * | 1996-05-17 | 1999-10-12 | ザ、プロクター、エンド、ギャンブル、カンパニー | 洗剤組成物 |
JP2000245499A (ja) * | 1999-02-26 | 2000-09-12 | Dainichiseika Color & Chem Mfg Co Ltd | 改良された化学発光試薬 |
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- 2008-10-08 JP JP2008261575A patent/JP5359169B2/ja not_active Expired - Fee Related
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
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JPH11511789A (ja) * | 1996-05-17 | 1999-10-12 | ザ、プロクター、エンド、ギャンブル、カンパニー | 洗剤組成物 |
JP2000245499A (ja) * | 1999-02-26 | 2000-09-12 | Dainichiseika Color & Chem Mfg Co Ltd | 改良された化学発光試薬 |
Non-Patent Citations (1)
Title |
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JPN6013038581; ANALYTICAL SCIENCES Vol. 16, 2000, pp.1249-1254 * |
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