JP2010046023A - Solid lactic acid fermented feed, method for producing the same, and apparatus for producing the feed - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a solid lactic acid fermented feed containing lactobacillus in high concentration: to provide a method for efficiently producing the feed: and to provide an apparatus for producing the feed. <P>SOLUTION: The solid lactic acid fermented feed is produced by subjecting a mixture of crushed vegetable wastes and moisture-adjusting dry raw material such as bran to increase the lactobacillus so as to have a viable cell count of more than 1×10<SP>9</SP>cfu/g. The method for producing the feed comprises: crushing the vegetable wastes V with a crusher 1; sterilizing the vegetable wastes with a sterilizer 2, introducing part of the sterilized vegetable wastes into a lactobacillus-culturing container 3 to culture the lactobacillus; mixing the cultured lactobacillus material, the remaining part of the sterilized vegetable wastes, and the moisture-adjusting dry raw material such as bran F together at least using a mixer 4 to make them a solid-state mixture; filling the solid-state mixture in a bag 9 using a bagging machine 5 and also degassing the inside of the bag or exchanging the air in the bag for nitrogen gas; sealing the bag; and subjecting the mixture to lactic acid fermentation to increase the viable cell count so as to be more than 1×10<SP>9</SP>cfu/g. <P>COPYRIGHT: (C)2010,JPO&INPIT

Description

  The present invention relates to a solid lactic acid fermented feed in which a specific food waste is lactically fermented and lactic acid bacteria are remarkably grown, and a manufacturing method and manufacturing equipment thereof.

In recent years, lactic acid fermented feeds have attracted attention as functional feeds that can be expected to suppress inflammation, improve immunity, improve protein metabolism, etc., and research to produce lactic acid fermented feeds effectively using food waste Has come to be done. Such a functional lactic acid fermented feed preferably contains lactic acid bacteria at a high density, and in particular, a lactic acid fermented feed having a viable count exceeding 1.0 × 10 ⁹ cfu / g is extremely suitable.

However, solid lactic acid fermented feed made from food waste such as tofu cake and bran is not so active in the growth of lactic acid bacteria, so that the number of viable bacteria exceeds 1.0 × 10 ⁹ cfu / g. It is difficult to ferment. Moreover, even in silage that is currently of good quality, the number of living lactic acid bacteria is about 1.0 × 10 ⁷ cfu / g to 1.0 × 10 ⁸ cfu / g, and there is no denying the lack of lactic acid bacteria.

  By the way, as one of the fermented feed made from food waste, it contains food waste (edible granules) such as bran, bran, oil cake, agricultural production residue, lactobacilli and actinomycetes It has been proposed to mix a living bacterial solution to be fermented under anaerobic conditions at 10 to 35 ° C. (Patent Document 1). This fermented feed is said to have the effect of reducing the odor of excrement and promoting composting of excrement, but the number of lactic acid bacteria has not been measured, It is estimated that the number of lactic acid bacteria in the lactic acid fermentation feed made from food waste such as tofu and bran is the same as or less than that.

As described above, in the conventional solid lactic acid fermented feed, there are no stable lactic acid bacteria that exceed 1.0 × 10 ⁹ cfu / g. There is a growing demand in the industry for the early development of a highly functional solid lactic acid fermentation feed containing lactic acid bacteria at a higher concentration than ever before.
JP-A-11-243867

  The present invention has been made under the circumstances described above, and the first problem to be solved is to select at least two kinds of specific wastes from food wastes, and by subjecting the mixture to lactic acid fermentation, The object is to provide a solid lactic acid fermented feed containing lactic acid bacteria at a higher concentration than before.

  The second and third problems to be solved by the present invention are to provide an efficient production method of the solid lactic acid fermentation feed and production equipment for carrying out this production method. .

In order to solve the first problem, the solid lactic acid fermentation feed according to the present invention is a lactic acid fermentation of a mixture of crushed vegetable waste and dry ingredients for moisture adjustment such as bran, and the number of viable bacteria is stable. Thus, it is grown to exceed 1 × 10 ⁹ cfu / g.

  In the solid lactic acid fermented feed of the present invention, it is preferable that tofu cake and waste syrup are further mixed with a mixture of vegetable waste and dry ingredients for moisture adjustment such as bran, and further that minerals are also mixed. preferable. The tofu cake is preferably one that is sealed and stored by spraying baker's yeast and sugar, and the lactic acid bacterium is preferably Lactobacillus plantarum. Moreover, it is preferable that the solid lactic acid fermented feed of the present invention is filled with a bag and sealed, and the bag is degassed or replaced with nitrogen gas.

Next, the production method of the present invention that solves the second problem is to crush and sterilize vegetable waste, to cultivate lactic acid bacteria with a part of the sterilized vegetable waste, The remainder and dry ingredients for moisture adjustment such as bran are mixed to form a solid mixture. The solid mixture is filled into a bag and the bag is degassed or replaced with nitrogen gas and sealed. It is fermented to grow lactic acid bacteria so that the number of viable bacteria exceeds 1 × 10 ⁹ cfu / g.

  In the production method of the present invention, it is preferable to sterilize the crushed vegetable waste by adding waste syrup, and in addition to the lactic acid bacteria culture, the remainder of the sterilized vegetable waste, and dry ingredients for moisture adjustment such as bran It is preferable to further mix the tofu cake and / or mineral.

  Furthermore, the production facility of the present invention that solves the third problem includes a crusher that crushes vegetable waste, a sterilizer that sterilizes the crushed vegetable waste, and cultures lactic acid bacteria by introducing a portion of the sterilized vegetable waste. A lactic acid bacteria culture device, a mixer that mixes the lactic acid bacteria culture, the remainder of the sterilized vegetable waste, dry ingredients for moisture adjustment such as bran, and the like, and fills the bag with the solid mixture and fills the bag And a bagging machine for sealing by replacing with degassing or nitrogen gas.

Since the solid lactic acid fermented feed of the present invention is mixed with moisture-controlling dry materials such as bran, the water in the feed is moderately adjusted (preferably about 50%) by the water-controlling dry materials and becomes solid. . And in order to mix the crushed vegetable waste, when lactic acid fermentation is carried out, lactic acid bacteria actively proliferate, and the viable count of lactic acid bacteria exceeds 1 × 10 ⁹ cfu / g despite being a solid feed. Thus, as supported by the experimental results (FIG. 3) described later, the number of viable bacteria becomes maximum when about 5 days have elapsed after the start of culture. The high density state in which the viable count exceeds 1 × 10 ⁹ cfu / g is maintained for about 10 days after the start of culture. Therefore, when the solid lactic acid fermented feed of the present invention is fed to livestock or the like by the time when 10 days have passed, various lactic acid bacteria contained at a high concentration, such as suppression of inflammation, improvement of immune ability, improvement of protein metabolism, etc. The function can be expected. Mixing the vegetable waste, as described above, in solid form lactic fermentation feed, lactic acid bacteria fact that actively growing beyond viable cell number 1 × 10 9 ^cfu / g was first findings by the present inventors Therefore, the solid lactic acid fermented feed of the present invention and the production method thereof are completed based on this finding.

  In the solid lactic acid fermented feed of the present invention, when the tofu koji and waste syrup are further mixed with a mixture of vegetable waste and dry ingredients for moisture adjustment such as bran, the protein nitrogen increases due to the tofu koji and the nutritional value In addition, lactic acid fermentation is promoted by waste syrup, and the growth of lactic acid bacteria becomes more active. Further, when minerals are mixed, the balance of trace essential components of the solid lactic acid fermented feed is improved, and mineral deficiency can be prevented. Minerals are also important components for culturing lactic acid bacteria.

  When tofu koji is mixed as described above, it is difficult to rot during storage because the tofu koji is sprayed with baker's yeast and sugar and stored in a sealed state. In addition, there is no need to adjust the temperature and moisture during storage, so that storage facilities can be simplified and energy costs can be reduced. In addition, about the preservation | save method of food residues, such as this tofu cake, since this applicant has applied for a patent previously (Japanese Patent Application No. 2005-10111, Unexamined-Japanese-Patent No. 2006-197809), description beyond this is abbreviate | omitted.

In the solid lactic acid fermented feed of the present invention, when Lactobacillus plantarum is used as a lactic acid bacterium, a solid lactic acid fermented feed having high palatability for cattle can be obtained. In addition, when the solid lactic acid fermented feed of the present invention is filled in a bag and sealed, and the bag is degassed or replaced with nitrogen gas, lactic acid bacteria actively proliferate in the bag as described above, and live bacteria The number exceeds 1.0 × 10 ⁹ cfu / g, and this high concentration state is maintained for about 10 days.

Next, as in the production method of the present invention, the lactic acid bacteria are cultured in a part of the crushed and sterilized vegetable waste, the lactic acid bacteria culture, the remainder of the sterilized vegetable waste, and dry ingredients for moisture adjustment such as bran Is mixed, it is economical because the amount of inoculum (commercial viable agent) is reduced by the amount of lactic acid bacteria cultured and mixed. And when filling the bag with the solid mixture and sealing the inside of the bag by degassing or replacing with nitrogen gas, lactic acid fermentation in the bag and growing the lactic acid bacteria to exceed 1 × 10 ⁹ cfu / g, There is no need for large-scale special fermentation equipment, and there is an advantage that it can be shipped immediately while being packed.

  In the production method of the present invention, when waste syrup is added to the crushed vegetable waste and sterilized, culturing of lactic acid bacteria is promoted by the waste syrup in the next lactic acid bacteria culture step, and a lactic acid bacteria culture containing high concentrations of lactic acid bacteria can be obtained. it can. And in addition to this lactic acid bacteria culture, the remainder of the sterilized vegetable waste, and dry ingredients for moisture adjustment such as bran, when tofu cake and / or minerals are further mixed, the nutritional value contains a large amount of protein nitrogen. A high solid lactic acid fermented feed and a solid lactic acid fermented feed with a good balance of trace essential components can be obtained.

  Moreover, like the production equipment of the present invention, a crusher that crushes vegetable waste, a sterilizer that sterilizes the crushed vegetable waste, a lactic acid bacteria culture device that introduces a part of the sterilized vegetable waste and cultures lactic acid bacteria, , Lactic acid bacteria culture, the remainder of sterilized vegetable waste and dry ingredients for moisture adjustment such as bran to make a solid mixture, filling the bag with the solid mixture and degassing or nitrogen in the bag The one provided with a bagging machine that replaces and seals with gas has an advantage that a special facility is not required, a manufacturing facility can be easily and inexpensively constructed, and can be easily implemented.

  Hereinafter, specific embodiments of the present invention will be described in detail.

Representative examples of the solid lactic acid fermented feed of the present invention are crushed and heat-sterilized vegetable scraps and dried raw materials for moisture adjustment such as bran, adding tofu cake and waste syrup to these main materials, The raw material and the lactic acid bacteria culture are mixed uniformly, the bag is filled with this solid mixture, the bag is degassed or replaced with nitrogen gas, sealed, and lactic acid fermented in the bag to produce the lactic acid bacteria. It was grown so that the number of bacteria exceeded 1 × 10 ⁹ cfu / g. In this lactic acid fermented feed, minerals may be mixed in addition to the above raw materials, and in some cases, crushed grass and corn may be mixed.

Vegetable waste is a very important raw material in order to actively growing as lactic acid bacteria that number of viable bacteria is more than 1 × 10 9 ^cfu / g, various are obtained cabbage, Chinese cabbage, as other food residue Vegetable waste can be used, and fruit waste can be included. This vegetable scrap is preferably used after being crushed into chips having a particle size of 5 mm or less and sterilized by heating at a temperature near 85 ° C. for about 30 minutes. In addition, dry ingredients for moisture adjustment such as bran are necessary for obtaining solid lactic acid fermented feed. In addition to wheat bran obtained on the market, rice bran, rice flour, flour, dried tofu, etc. Can also be used.

  Tofu cake is mixed for the purpose of its effective use and increasing the nutritional value of the feed. As described above, baker's yeast and sugar are sprayed on the surface and put into a vinyl bag for storage and sealed. Preserved tofu cake that is resistant to spoilage is preferably used. The waste syrup is mixed for the purpose of promoting lactic fermentation and activating the growth of lactic acid bacteria, and any of various fruit syrups discharged from a fruit processing factory is preferably used. In addition, the dry raw materials for moisture adjustment, such as tofu cake, waste syrup, and the above bran, are preferably used after being sterilized by heating at a temperature around 120 ° C. for about 20 minutes.

  In addition, minerals are important for preventing the deficiency of livestock minerals by improving the balance of trace essential components in feeds, and also important for cultivating lactic acid bacteria, such as zinc, potassium and calcium. A small amount of chromium, selenium, iron, copper, sodium, magnesium, manganese, iodine, phosphorus, etc. are mixed. In particular, calcium is a constituent element of bone tissue of livestock and chelate lactic acid and acetic acid generated when cultivating lactic acid bacteria to prevent a rapid drop in pH and promote the cultivation of lactic acid bacteria. is important.

  The mixing ratio of each raw material is not particularly limited, but attention should be paid to the mixing ratio of waste syrup. That is, waste syrup is important as an energy source for actively growing lactic acid bacteria, but as can be seen from the experimental results (FIG. 1) described later, in the feed containing about 50% by weight of water, the mixing ratio of waste syrup is When the amount exceeds 50% by mass, the high osmotic pressure suppresses the growth of lactic acid bacteria. Therefore, the feed solid content is 50% by mass or less (25 parts by mass or less with respect to 50% by mass of feed solids), preferably 4 to It is important to set to about 20% by mass (about 2 to 10 parts by mass with respect to 50 parts by mass of feed solids).

Vegetable scraps that play an important role in the growth of lactic acid bacteria do not need to be mixed in large amounts. As can be seen from the results of Experiments 1 to 3 described later, about 5% by weight of feed solids (relative to 50 parts by weight of feed solids) About 2.5 parts by mass), the lactic acid bacteria can be grown at a high density exceeding 1 × 10 ⁹ cfu / g. Moreover, it is appropriate to set the mixing ratio of the dry ingredients for moisture adjustment such as bran to about 36 to 48% by mass of feed solids (about 18 to 24 parts by mass with respect to 50 parts by mass of feed solids). The mixing ratio of the tofu cake is also set to be substantially the same as that of the moisture adjusting dry raw material.

Lactobacillus plantarum (Lactobacillus plantarum) is preferably used as the lactic acid bacterium, which can stably and rapidly grow under anaerobic conditions at room temperature and can obtain a lactic acid fermented feed having high palatability for cattle. The concentration of lactic acid bacteria mixed (inoculated) with the above-mentioned raw material is sufficient if it is about 1 × 10 ⁶ cfu / g to 5 × 10 ⁷ cfu / g, as can be seen from the experimental results described later (FIG. 2). to, the 48 hours after even minimum inoculation density 1 × ¹⁰ 6 cfu / g to grow to more than 1 × ¹⁰ 9 cfu / g.

This solid lactic acid fermented feed is prepared by mixing lactic acid bacteria having the above-mentioned concentration and the above raw materials, filling the solid mixture into a bag and sealing the bag by degassing or replacing it with nitrogen gas, and anaerobic in the bag. Under the lactic acid fermentation at room temperature below, when fermented in the bag in this way, as can be seen from the experimental results (FIG. 3) described later, the lactic acid bacteria rapidly grow in a short time and the number of viable bacteria is 1 It exceeds × 10 ⁹ cfu / g and continues to grow for about 5 days, and the number of viable bacteria reaches a peak. And after passing the peak, the number of viable bacteria decreases rapidly, and the above high concentration is maintained until about 10 days, but the number of viable cells is less than 1 × 10 ⁵ cfu / g after about 3 weeks. It becomes impossible to detect. Therefore, when this packed solid lactic acid fermented feed is fed to livestock and the like by about 10 days, various lactic acid bacteria contained at a high concentration can suppress inflammation, improve immunity, improve protein metabolism, etc. The function of can be expected.

  Next, an experiment conducted on the solid lactic acid fermented feed of the present invention will be described.

[Experiment 1]
Tofu cake, cabbage waste, waste syrup and wheat bran are mixed at the mixing ratio shown in Table 1 below to produce four types of media (1), (2), (3) and (4) containing 50% by mass of water. did. The tofu cake used was a product with baker's yeast and sugar spread on the surface, the waste syrup was chestnut syrup, and the cabbage waste was crushed to a particle size of 5 mm or less. Tofu cake, waste syrup and bran were heat sterilized at 120 ° C. for 20 minutes using an autoclave, and cabbage waste was heat sterilized at 85 ° C. for 30 minutes.

Lactic acid bacteria [Lactobacillus plantarum (NBRC3070)] grown in MRS liquid medium were inoculated into each of the above media so as to be 5 × 10 ⁷ cfu / g, filled in light-shielding plastic bags and bags. The inside was deaerated and sealed, and cultured for 24 hours under anaerobic conditions, protected from light at 24 ° C. After culturing, the number of viable bacteria in each medium was measured by a colony count method using an MRS agar medium. The result is shown in the graph of FIG.

As shown in FIG. 1, in the mediums (1), (2), and (3) in which the mixing ratio of waste syrup accounts for 4 to 50% by mass of the medium excluding moisture, lactic acid bacteria are 1 × 10 ⁹ cfu / g in 24 hours. The lactic acid bacteria of the culture medium (1) in which the density of lactic acid bacteria which grows in the above-mentioned high density, and the mixing ratio of waste syrup occupies 20% by mass is high, and then the mixing ratio of waste syrup accounts for 4% by mass. The density of lactic acid bacteria decreased in the medium (3), which has a high density and the mixing ratio of waste syrup accounts for 50% by mass. And the culture medium (4) containing only waste syrup could not be detected because the growth of lactic acid bacteria was hindered due to the high osmotic pressure, and it was 1 × 10 ⁵ cfu / g or less.

  From this experimental result, it can be seen that the preferable mixing ratio of the waste syrup is in the range of 4 to 20% by mass as described above.

[Experiment 2]
Lactic acid bacteria [Lactobacillus plantarum (NBRC3070)] prepared in three mediums (1) in Table 1 above and cultured in an MRS liquid medium were diluted with PBS to 1 × 10 ⁶ cfu / g, 5 × 10 respectively. Inoculate the three media (1) to ⁶ cfu / g and 5 × 10 ⁷ cfu / g, and incubate for 48 hours under the same conditions as in Experiment 1. The number of viable bacteria after 24 hours and 48 hours The number of viable bacteria at the time was measured in the same manner as in Experiment 1. The result is shown in the graph of FIG.

As shown in FIG. 2, the lowest inoculum density of 1 × 10 ⁶ cfu / g has significantly fewer viable cell counts than other higher inoculation density at 24 hours. Although it is slightly lower than 10 ⁹ cfu / g, when 48 hours have passed, the difference in the number of viable bacteria from other high inoculation density is greatly reduced, exceeding 1 × 10 ⁹ cfu / g. It was proliferating. Therefore, even when inoculated with about 1 × 10 ⁶ cfu / g lactic acid bacteria, a lactic acid fermented feed in which the lactic acid bacteria are grown at a high density of 1 × 10 ⁹ cfu / g or more can be produced. It can be seen that the use of the agent can be saved.

[Experiment 3]
The medium (1) shown in Table 1 was prepared, and lactic acid bacteria [Lactobacillus plantarum (NBRC3070)] were inoculated into the medium (1) so as to be 5 × 10 ⁷ cfu / g as in Experiment 1. This was filled in a light-shielding plastic bag and the inside of the bag was deaerated and sealed, and the culture was continued for 4 weeks under light-shielded and anaerobic conditions at 24 ° C. Then, the number of viable bacteria in the medium was measured by the colony count method on the way, and the daily change of the viable cell count was examined. The result is shown in FIG.

As shown in FIG. 3, the number of viable bacteria increased rapidly during 1 to 2 days of culture and exceeded 1 × 10 ⁹ cfu / g, and continued to increase thereafter until reaching a peak at about 5 days of culture. However, the viable cell count rapidly decreases until about 10 days of culture, and a high concentration of 1 × 10 ⁹ cfu / g or more is maintained until about 10 days of culture. After 3 weeks, the viable cell count becomes 1 × 10 ⁵ cfu / g or less. Will not be detected. Therefore, it can be seen that when fed by about 10 days of culture with a high density of lactic acid bacteria, various functions such as suppression of inflammation, improvement of immune ability, and improvement of protein metabolism can be expected by lactic acid bacteria contained at a high concentration.

[Experiment 4]
27 parts by mass of cabbage scraps and 23 parts by mass of bran were mixed to prepare a medium (water content of 50% by mass) consisting only of essential raw materials. In this medium, lactic acid bacteria [Lactobacillus plantarum (NBRC3070)] were inoculated to 5 × 10 ⁷ cfu / g in the same manner as in Experiment 1 and cultured for 48 hours under the same conditions as in Experiment 1 for 24 hours. The number of viable cells at the time of passage and the number of viable cells at the time of 48 hours were measured in the same manner as in Experiment 1. As a result, the viable cell count after 6.5 hours was 6.525 × 10 ⁹ cfu / g, and the viable cell count after 48 hours was 7.075 × 10 ⁹ cfu / g.
This shows that it is possible to obtain a lactic acid fermented feed containing lactic acid bacteria at a high density of 1 × 10 ⁹ cfu / g or more only with vegetable waste (cabbage waste) and bran (dry raw material for moisture adjustment). .

  Next, with reference to FIG. 4, the manufacturing method and manufacturing equipment of the solid lactic acid fermented feed which concern on one Embodiment of this invention are demonstrated.

  The manufacturing equipment shown in FIG. 4 includes a crusher 1 that crushes vegetable waste into chips having a particle size of 5 mm or less, a sterilizer 2 that introduces the crushed vegetable waste and waste syrup and sterilizes by heat, and this Lactic acid bacteria incubator 3 for cultivating lactic acid bacteria by introducing sterilized vegetable waste and waste syrup, this lactic acid bacteria culture, the remainder of sterilized vegetable waste and waste syrup, dry ingredients for moisture adjustment such as bran, and tofu The mixing machine 4 which mixes a soot etc. to make a solid mixture, the bagging machine 5 which fills this solid mixture into a bag and deaerates and seals the inside of the bag are provided.

  According to this manufacturing facility, first, vegetable scraps V are put into the crusher 1 and crushed into chips having a particle size of 5 mm or less. The crushed vegetable waste V is introduced into the sterilizer 2, mixed with the waste syrup S also introduced into the sterilizer 2, and sterilized by heating at 85 ° C. for 30 minutes. This sterilizer 2 is for heat sterilization with the heat of steam supplied from a steam boiler 6. The vegetable waste V may contain fruit waste.

Part of the sterilized vegetable waste and waste syrup is introduced into the lactic acid bacteria incubator 3 and the remainder is supplied to the mixer 4. The vegetable scraps and waste syrup introduced into the lactic acid bacteria culture machine 3 are cultured at room temperature (20 to 25 ° C.) for 24 hours under nitrogen gas sent from a nitrogen gas cylinder 7. Thus, when culture | cultivation is performed for 24 hours, the lactic acid bacteria culture (liquid) whose lactic acid bacteria count (viable bacteria count) is about 1 * 10 < ⁶ > cfu / g-1 * 10 < ⁸ > cfu / g is obtained. This lactic acid bacteria culture device 3 has a capacity twice that of the sterilizer 2 and after 24 hours of cultivation, half of the lactic acid bacteria culture is supplied to the mixer 4 and the remaining half of the lactic acid bacteria culture is sterilized. The vegetable waste and waste syrup supplied from the vessel 2 are added and the culture is repeated. Therefore, a lactic acid bacterium culture having the above-mentioned density can be obtained only by replenishing a commercially available inoculum (viable agent) about once a week, so that the inoculum can be saved, which is economically advantageous.

  Half of the lactic acid bacteria culture (liquid) is supplied to the mixer 4, this lactic acid bacteria culture, vegetable waste and syrup also supplied to the mixer 4, bran F (dry material for moisture adjustment), and tofu cake T Are uniformly mixed at the mixing ratio described above to obtain a solid mixture having a water content of about 50% by weight. Moreover, a mineral is also mixed as needed. Although not shown in FIG. 4, it is desirable to provide a heating device such as an autoclave and sterilize by heating at 120 ° C. for 20 minutes before mixing the bran F and tofu cake T.

The solid mixture is supplied from the mixer 4 to the hopper 5a of the bagging machine 5 by the transporting conveyor 8, filled into the light-shielding plastic bag 9 by the bagging machine 5, and the inside of the bag is degassed to produce the plastic. The bag 9 is sealed. When packed in this manner, lactic acid fermentation is carried out in the bag, and as described above, lactic acid bacteria actively proliferate exceeding 1 × 10 ⁹ cfu / g, and a solid lactic acid fermented feed containing lactic acid bacteria at high density is obtained. Since this high density state is maintained for about 10 days, a high density lactic acid fermented feed can be fed to livestock if shipped immediately after bagging.

  In this embodiment, a bag filling machine 5 that degass the air in the bag is adopted, but instead, a bag filling machine that replaces the air in the bag with nitrogen gas is adopted. Of course.

  As described above, the production method and production equipment of the present invention do not require a special device or an expensive device, and furthermore, a solid lactic acid fermented feed containing lactic acid bacteria at high density by lactic acid fermentation in a bag. Therefore, a large-scale fermentation facility is not required, it can be shipped immediately in a bag, and the inoculum (commercial viable agent) can be saved, making it economical and easy to implement.

It is a graph which shows the result of Experiment 1 (change of lactic acid bacteria growth when the mixing ratio of waste syrup is changed). It is a graph which shows the result (The lactic acid bacteria density | concentration at the time of culture | cultivation start, and the lactic acid bacteria density | concentration after 48 hours after 48 hours) of the experiment 2. It is a graph which shows the result of Experiment 3 (Annual change of the number of lactic acid bacteria (viable bacteria number) in the culture medium in long-term culture). It is explanatory drawing of the manufacturing method and manufacturing apparatus which concern on one Embodiment of this invention.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Crusher 2 Sterilizer 3 Lactic acid bacteria culture device 4 Mixer 5 Bag filling machine V Vegetable waste F Bran (Dry raw material for moisture adjustment)
T tofu cake S waste syrup

Claims (10)

A mixture of crushed vegetable waste and dry ingredients for moisture adjustment such as bran is subjected to lactic acid fermentation, and the lactic acid bacteria are grown so that the viable cell count exceeds 1 × 10 ⁹ cfu / g. Shape lactic fermented feed.   The solid lactic acid fermented feed according to claim 1, wherein the mixture is further mixed with tofu cake and waste syrup.   The solid lactic acid fermented feed according to claim 1 or 2, wherein a mineral is further mixed in the mixture.   The solid lactic acid fermented feed according to claim 2, wherein the tofu koji is sealed and stored by spraying baker's yeast and sugars.   The solid lactic acid fermented feed according to claim 1, wherein the lactic acid bacterium is Lactobacillus plantarum.   The solid lactic acid fermented feed according to any one of claims 1 to 5, wherein the bag is filled and sealed, and the inside of the bag is degassed or replaced with nitrogen gas. The vegetable waste is crushed and sterilized, and lactic acid bacteria are cultured in a portion of the sterilized vegetable waste. The lactic acid bacteria culture, the remainder of the sterilized vegetable waste, and dry ingredients for moisture adjustment such as bran are mixed and solidified. Fill the bag with this solid mixture and seal the bag by degassing or replacing it with nitrogen gas. The bag is sealed with lactic acid and the number of viable lactic acid bacteria is 1 × 10 ⁹ cfu / g. A method for producing a solid lactic acid fermented feed, characterized in that it is allowed to grow so as to exceed.   The manufacturing method according to claim 7, wherein waste syrup is added to the crushed vegetable waste and sterilized.   The tofu cake and / or mineral is further mixed in addition to the lactic acid bacteria culture, the remainder of the sterilized vegetable waste, and dry ingredients for moisture adjustment such as bran. Production method. A crusher that crushes vegetable waste, a sterilizer that sterilizes the crushed vegetable waste, a lactic acid bacteria culture device that introduces a portion of the sterilized vegetable waste and cultures lactic acid bacteria, and the remainder of the sterilized vegetable waste and sterilized vegetable waste A blender that mixes dry ingredients for moisture adjustment such as bran and a solid mixture, and a bagging machine that fills the bag with the solid mixture and seals the bag by degassing or replacing it with nitrogen gas A facility for producing a solid lactic acid fermented feed, comprising:

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