JP2009537165A - 最適化されたグリコシル化活性を有するキメラ・グリコシルトランスフェラーゼをコードする遺伝子配列の製造及び使用 - Google Patents

最適化されたグリコシル化活性を有するキメラ・グリコシルトランスフェラーゼをコードする遺伝子配列の製造及び使用 Download PDF

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JP2009537165A
JP2009537165A JP2009511529A JP2009511529A JP2009537165A JP 2009537165 A JP2009537165 A JP 2009537165A JP 2009511529 A JP2009511529 A JP 2009511529A JP 2009511529 A JP2009511529 A JP 2009511529A JP 2009537165 A JP2009537165 A JP 2009537165A
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seq
nucleotide sequence
delimited
sites
sequence
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JP2009537165A5 (https=
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ローニン，カトリーヌ
ギロディ−カプラズ，ガエル
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Aix Marseille Universite
Centre National de la Recherche Scientifique CNRS
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Centre National de la Recherche Scientifique CNRS
Universite de Provence Aix Marseille I
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Publication of JP2009537165A publication Critical patent/JP2009537165A/ja
Publication of JP2009537165A5 publication Critical patent/JP2009537165A5/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1081—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01087—N-Acetyllactosaminide 3-alpha-galactosyltransferase (2.4.1.87), i.e. alpha-1,3-galactosyltransferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01133—Xylosylprotein 4-beta-galactosyltransferase (2.4.1.133)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/99—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
    • C12Y204/99001—Beta-galactoside alpha-2,6-sialyltransferase (2.4.99.1)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/05—Fusion polypeptide containing a localisation/targetting motif containing a GOLGI retention signal

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2009511529A 2006-05-24 2007-05-24 最適化されたグリコシル化活性を有するキメラ・グリコシルトランスフェラーゼをコードする遺伝子配列の製造及び使用 Pending JP2009537165A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP06290843 2006-05-24
PCT/EP2007/055070 WO2007135194A2 (en) 2006-05-24 2007-05-24 Preparation and uses of gene sequences encoding chimerical glycosyltransferases with optimized glycosylation activity

Publications (2)

Publication Number Publication Date
JP2009537165A true JP2009537165A (ja) 2009-10-29
JP2009537165A5 JP2009537165A5 (https=) 2010-07-15

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JP2009511529A Pending JP2009537165A (ja) 2006-05-24 2007-05-24 最適化されたグリコシル化活性を有するキメラ・グリコシルトランスフェラーゼをコードする遺伝子配列の製造及び使用

Country Status (10)

Country Link
US (1) US8993297B2 (https=)
EP (1) EP2019864B1 (https=)
JP (1) JP2009537165A (https=)
AT (1) ATE516346T1 (https=)
CA (1) CA2653104C (https=)
DK (1) DK2019864T3 (https=)
ES (1) ES2368267T3 (https=)
PL (1) PL2019864T3 (https=)
PT (1) PT2019864E (https=)
WO (1) WO2007135194A2 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2016520315A (ja) * 2013-05-29 2016-07-14 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ シアル酸付加の定量的調節

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3017057B1 (en) 2013-07-05 2019-10-02 F.Hoffmann-La Roche Ag Process for the mono- and bi-sialylation of glycoproteins employing n-terminally truncated beta-galactoside alpha-2,6-sialyltransferase mutants
EP2821482A1 (en) * 2013-07-05 2015-01-07 Roche Diagniostics GmbH N-terminally truncated glycosyltransferases
EP2824176A1 (en) 2013-07-11 2015-01-14 Siamed'xpress Methods for producing sialylated therapeutic proteins
US9616114B1 (en) 2014-09-18 2017-04-11 David Gordon Bermudes Modified bacteria having improved pharmacokinetics and tumor colonization enhancing antitumor activity
WO2016102436A1 (en) * 2014-12-22 2016-06-30 F. Hoffmann-La Roche Ag Cmp-dependent sialidase activity
US10676723B2 (en) 2015-05-11 2020-06-09 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
PL240405B1 (pl) * 2017-07-24 2022-03-28 Zakl Farmaceutyczne Polpharma Spolka Akcyjna Przeciwciało monoklonalne oraz sposób jego otrzymywania
BR112022013071A2 (pt) * 2020-01-06 2022-09-20 Igm Biosciences Inc Moléculas de ligação multiméricas altamente sialiladas
WO2023141513A2 (en) * 2022-01-19 2023-07-27 The Regents Of The University Of California Functionalized human milk oligosaccharides and methods for producing them
WO2025088103A1 (en) * 2023-10-26 2025-05-01 Inbiose N.V. Production of a sialylated compound
WO2025088102A1 (en) * 2023-10-26 2025-05-01 Inbiose N.V. PRODUCTION OF A SIALYLATED OLIGOSACCHARIDE COMPRISING GAL-β1,3-[NEU5AC-A2,6]-HEXNAC-R

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ATE374816T1 (de) 1996-08-02 2007-10-15 Austin Research Inst Verbesserte nukleinsäuren, welche für eine chimäre glycosyltransferase kodieren
US7244601B2 (en) * 1997-12-15 2007-07-17 National Research Council Of Canada Fusion proteins for use in enzymatic synthesis of oligosaccharides
US7598055B2 (en) * 2000-06-28 2009-10-06 Glycofi, Inc. N-acetylglucosaminyltransferase III expression in lower eukaryotes
ES2252261T3 (es) 2000-06-28 2006-05-16 Glycofi, Inc. Metodos para producir glicoproteinas modificadas.
AU2002352253A1 (en) * 2001-12-18 2003-06-30 Bayer Healthcare Ag Regulation of human sialyltransferase
US7569376B2 (en) * 2002-05-03 2009-08-04 Neose Technologies, Inc. Fucosyltransferase fusion protein

Non-Patent Citations (6)

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JPN6012036706; Ｍｅｔａｂ．　Ｅｎｇ． Ｖｏｌ．７, 2005, ｐ．２２１-２２８ *
JPN6012036707; Ｊ．　Ｂｉｏｌ．　Ｃｈｅｍ． Ｖｏｌ．２７４，　Ｎｏ．５１, 1999, ｐ．３６１０７-３６１１６ *
JPN6012036708; Ｊ．　Ｂｉｏｌ．　Ｃｈｅｍ． Ｖｏｌ．２６８，　Ｎｏ．１３, 1993, ｐ．９９０８-９９１６ *
JPN6012036709; Ｊ．　Ｂｉｏｌ．　Ｃｈｅｍ． Ｖｏｌ．２７６，　Ｎｏ．２４, 2001, ｐ．２１６０８-２１６１７ *
JPN6012036711; Ｂｉｏｃｈｉｍｉｅ Ｖｏｌ．８５, 2003, ｐ．３１１-３２１ *
JPN6012036712; Ｂｉｏｃｈｉｍ．　Ｂｉｏｐｈｙｓ．　Ａｃｔａ Ｖｏｌ．１５４９, 2001, ｐ．１６１-１７３ *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2016520315A (ja) * 2013-05-29 2016-07-14 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ シアル酸付加の定量的調節

Also Published As

Publication number Publication date
DK2019864T3 (da) 2011-09-26
PL2019864T3 (pl) 2012-02-29
US8993297B2 (en) 2015-03-31
ES2368267T3 (es) 2011-11-15
CA2653104A1 (en) 2007-11-29
CA2653104C (en) 2016-08-02
WO2007135194A2 (en) 2007-11-29
US20100105106A1 (en) 2010-04-29
ATE516346T1 (de) 2011-07-15
EP2019864A2 (en) 2009-02-04
EP2019864B1 (en) 2011-07-13
PT2019864E (pt) 2011-09-06
WO2007135194A3 (en) 2008-03-27
WO2007135194A9 (en) 2008-05-02

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