ATE516346T1 - Herstellung und verwendungen von chimäre glykosyltransferasen mit optimierter glykosylierungsaktivität codierenden gensequenzen - Google Patents

Herstellung und verwendungen von chimäre glykosyltransferasen mit optimierter glykosylierungsaktivität codierenden gensequenzen

Info

Publication number
ATE516346T1
ATE516346T1 AT07729499T AT07729499T ATE516346T1 AT E516346 T1 ATE516346 T1 AT E516346T1 AT 07729499 T AT07729499 T AT 07729499T AT 07729499 T AT07729499 T AT 07729499T AT E516346 T1 ATE516346 T1 AT E516346T1
Authority
AT
Austria
Prior art keywords
gene sequences
sequences encoding
glycosyltransferase
chimeric
preparation
Prior art date
Application number
AT07729499T
Other languages
English (en)
Inventor
Catherine Ronin
Gaelle Guiraudie-Capraz
Original Assignee
Univ Provence Aix Marseille 1
Centre Nat Rech Scient
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Provence Aix Marseille 1, Centre Nat Rech Scient filed Critical Univ Provence Aix Marseille 1
Application granted granted Critical
Publication of ATE516346T1 publication Critical patent/ATE516346T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1081—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01087—N-Acetyllactosaminide 3-alpha-galactosyltransferase (2.4.1.87), i.e. alpha-1,3-galactosyltransferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01133—Xylosylprotein 4-beta-galactosyltransferase (2.4.1.133)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/99—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
    • C12Y204/99001—Beta-galactoside alpha-2,6-sialyltransferase (2.4.99.1)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/05—Fusion polypeptide containing a localisation/targetting motif containing a GOLGI retention signal

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT07729499T 2006-05-24 2007-05-24 Herstellung und verwendungen von chimäre glykosyltransferasen mit optimierter glykosylierungsaktivität codierenden gensequenzen ATE516346T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP06290843 2006-05-24
PCT/EP2007/055070 WO2007135194A2 (en) 2006-05-24 2007-05-24 Preparation and uses of gene sequences encoding chimerical glycosyltransferases with optimized glycosylation activity

Publications (1)

Publication Number Publication Date
ATE516346T1 true ATE516346T1 (de) 2011-07-15

Family

ID=36659871

Family Applications (1)

Application Number Title Priority Date Filing Date
AT07729499T ATE516346T1 (de) 2006-05-24 2007-05-24 Herstellung und verwendungen von chimäre glykosyltransferasen mit optimierter glykosylierungsaktivität codierenden gensequenzen

Country Status (10)

Country Link
US (1) US8993297B2 (de)
EP (1) EP2019864B1 (de)
JP (1) JP2009537165A (de)
AT (1) ATE516346T1 (de)
CA (1) CA2653104C (de)
DK (1) DK2019864T3 (de)
ES (1) ES2368267T3 (de)
PL (1) PL2019864T3 (de)
PT (1) PT2019864E (de)
WO (1) WO2007135194A2 (de)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6594860B2 (ja) * 2013-05-29 2019-10-23 エフ．ホフマン−ラ ロシュ アーゲー シアル酸付加の定量的調節
EP3017057B1 (de) 2013-07-05 2019-10-02 F.Hoffmann-La Roche Ag Verfahren zur mono- und bi-sialylierung von glykoproteinen unter verwendung von n-terminal verkürzten mutanten der beta-galaktosid alpha-2,6-sialyltransferase
EP2821482A1 (de) * 2013-07-05 2015-01-07 Roche Diagniostics GmbH N-terminal verkürzte Glykosyltransferasen
EP2824176A1 (de) 2013-07-11 2015-01-14 Siamed'xpress Verfahren zur Herstellung von sialylierten therapeutischen Proteinen
US9616114B1 (en) 2014-09-18 2017-04-11 David Gordon Bermudes Modified bacteria having improved pharmacokinetics and tumor colonization enhancing antitumor activity
CN107429237B (zh) * 2014-12-22 2021-09-28 豪夫迈·罗氏有限公司 Cmp依赖性的唾液酸酶活性
US10676723B2 (en) 2015-05-11 2020-06-09 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria
PL240405B1 (pl) * 2017-07-24 2022-03-28 Zakl Farmaceutyczne Polpharma Spolka Akcyjna Przeciwciało monoklonalne oraz sposób jego otrzymywania
WO2021141902A1 (en) * 2020-01-06 2021-07-15 Igm Biosciences, Inc. Highly sialylated multimeric binding molecules
WO2023141513A2 (en) * 2022-01-19 2023-07-27 The Regents Of The University Of California Functionalized human milk oligosaccharides and methods for producing them
WO2025088103A1 (en) * 2023-10-26 2025-05-01 Inbiose N.V. Production of a sialylated compound
WO2025088102A1 (en) * 2023-10-26 2025-05-01 Inbiose N.V. PRODUCTION OF A SIALYLATED OLIGOSACCHARIDE COMPRISING GAL-β1,3-[NEU5AC-A2,6]-HEXNAC-R

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH11512933A (ja) 1996-08-02 1999-11-09 ザ オースティン リサーチ インスティテュート キメラグリコシルトランスフェラーゼをコード化する改良された核酸
US7244601B2 (en) * 1997-12-15 2007-07-17 National Research Council Of Canada Fusion proteins for use in enzymatic synthesis of oligosaccharides
ATE440959T1 (de) 2000-06-28 2009-09-15 Glycofi Inc Verfahren für die herstellung modifizierter glykoproteine
US7598055B2 (en) * 2000-06-28 2009-10-06 Glycofi, Inc. N-acetylglucosaminyltransferase III expression in lower eukaryotes
AU2002352253A1 (en) * 2001-12-18 2003-06-30 Bayer Healthcare Ag Regulation of human sialyltransferase
WO2003093448A2 (en) 2002-05-03 2003-11-13 Neose Technologies, Inc. Recombinant glycosyltransferase fusion proteins

Also Published As

Publication number Publication date
WO2007135194A3 (en) 2008-03-27
US20100105106A1 (en) 2010-04-29
WO2007135194A9 (en) 2008-05-02
EP2019864A2 (de) 2009-02-04
PT2019864E (pt) 2011-09-06
ES2368267T3 (es) 2011-11-15
EP2019864B1 (de) 2011-07-13
DK2019864T3 (da) 2011-09-26
CA2653104C (en) 2016-08-02
JP2009537165A (ja) 2009-10-29
US8993297B2 (en) 2015-03-31
WO2007135194A2 (en) 2007-11-29
PL2019864T3 (pl) 2012-02-29
CA2653104A1 (en) 2007-11-29

Similar Documents

Publication Publication Date Title
PL2019864T3 (pl) Wytwarzanie i zastosowania sekwencji genowych kodujących chimerowe glikozylotransferazy o optymalizowanej aktywności glikozylacji
Angata et al. Unique disulfide bond structures found in ST8Sia IV polysialyltransferase are required for its activity
ATE492633T1 (de) Polypeptide mit cellobiohydrolaseaktivität und dafür kodierende polynukleotide
NZ595495A (en) Cellulolytic enzymes, nucleic acids encoding them and methods for making and using them
BR112012006032A2 (pt) polipetídeo isolado, composição, célula hospedeira recombinante, métodos para produzir o polipetídeo, um polipetídeo tendo atividade realçadora celulolítica, um mutante de uma célula precursora, uma proteína e um produto de fermentação, planta transgênica, parte de planta ou célula de planta, molécula de rna inibidora de filamento duplo(rsrna), e, métodos para inibir a expressão de um polipetídeo tendo atividade realçadora celulolítica em uma célula, degradar ou converter um polipetídeo isolado, composição, célula hospedeira recombinante, métodos para produzir o polipetídeo, um polipetídeo tendo atividade realçadora celulolítica, um mutante de uma célula precursora, uma proteína e um produto de fermentação, planta transgênica, parte de planta ou célula de planta, molécula de rna inibidora de filamento duplo (dsrna), e, métodos para inibir a expressão de um polipetídeo tendo atividade realçadora celulolítica em uma célula, degradar ou converter um material celulósico e fermentar um material celulósico.
JP2009261399A5 (de)
MX2011012906A (es) Produccion recombinante de peptidos.
NZ601693A (en) Bispecific egfr/igfir binding molecules
EA201101553A1 (ru) Полипептид, деградирующий углеводы, и его применения
NZ593203A (en) Mammalian-type glycosylation in plants by expression of non-mammalian glycosyltransferases by inserting a polypeptide coding for a non-mammalian beta1,4-galactosyltransferase
JP2009537165A5 (de)
NZ596764A (en) N-glycan core beta-galactosyltransferase and uses thereof
WO2008151440A4 (en) Modifying glycoprotein production in plants
RU2010108308A (ru) Способ получения гетерогенных белков
BR112012009886A2 (pt) célula, e, método para produzir uma proteína recombinante em uma célula hospedeira
MX2011010494A (es) Polipeptidos que tienen actividad de celulasa.
RU2010119028A (ru) Удф-глюкуронилтрансфераза и кодирующий ее полинуклеотид
EP2533792A4 (de) Chemisches präparat aus polyubiquitinketten
ATE527345T1 (de) Chimäres fusionsprotein mit überlegenen chaperon- und faltungsaktivitäten
NZ590011A (en) Chloramphenicol acetyl transferase (cat)-defective somatostatin fusion protein and uses thereof
ATE515568T1 (de) Polypeptide mit endoglucanase-aktivtät und dafür codierende polynukleotide
BR112016005859A2 (pt) Heterotransglicosilase e usos da mesma
IN2012DN00448A (de)
ATE447013T1 (de) Polypeptide mit endoglucanase-aktivtät und dafür codierende polynukleotide
ATE445009T1 (de) Signalpeptid zur herstellung eines polypeptids

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification

Ref document number: 2019864

Country of ref document: EP