JP2009525044A - 間葉系幹細胞集合体を調製および特性評価する方法ならびにそれらの使用 - Google Patents

間葉系幹細胞集合体を調製および特性評価する方法ならびにそれらの使用 Download PDF

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JP2009525044A
JP2009525044A JP2008553314A JP2008553314A JP2009525044A JP 2009525044 A JP2009525044 A JP 2009525044A JP 2008553314 A JP2008553314 A JP 2008553314A JP 2008553314 A JP2008553314 A JP 2008553314A JP 2009525044 A JP2009525044 A JP 2009525044A
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モシェ・クーゲル
アダム・ジェイ・キャッツ
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ユニバーシティ オブ バージニア パテント ファウンデーション
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JP2008553314A 2006-01-30 2007-01-30 間葉系幹細胞集合体を調製および特性評価する方法ならびにそれらの使用 Pending JP2009525044A (ja)

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US76333306P 2006-01-30 2006-01-30
PCT/US2007/002572 WO2007089798A2 (en) 2006-01-30 2007-01-30 Methods of preparing and characterizing mesenchymal stem cell aggregates and uses thereof

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JP2009525044A true JP2009525044A (ja) 2009-07-09
JP2009525044A5 JP2009525044A5 (enExample) 2010-07-15

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US (1) US8592209B2 (enExample)
EP (1) EP1984488B1 (enExample)
JP (1) JP2009525044A (enExample)
KR (1) KR20080091832A (enExample)
AU (1) AU2007209870A1 (enExample)
CA (1) CA2641022A1 (enExample)
WO (1) WO2007089798A2 (enExample)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010505849A (ja) * 2006-10-06 2010-02-25 ユニバーシティ オブ バージニア パテント ファウンデーション 糖尿病性創傷治癒に有用な方法および組成物
JP2012502636A (ja) * 2008-09-22 2012-02-02 ウニヴェルジテート チューリッヒ プロレクトラート エムエヌヴェー 懸滴プレート

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100322906A1 (en) 2005-10-05 2010-12-23 Osaka University Method for Obtaining Pancreatic Endocrine Cells From Adipose Tissue-Origin Cells
EP1984488B1 (en) 2006-01-30 2017-12-27 University Of Virginia Patent Foundation Methods of preparing and characterizing mesenchymal stem cell aggregates and uses thereof
US10633632B2 (en) * 2007-07-12 2020-04-28 Discgenics, Inc. Human disc tissue
US20120100607A1 (en) * 2007-07-12 2012-04-26 Christopher Duntsch Compositions of adult disc stem cells and methods for the treatment of degenerative disc disease
US8227246B2 (en) 2007-07-12 2012-07-24 Discgenics Compositions of adult disc stem cells for the treatment of degenerative disc disease
US8017390B2 (en) 2007-07-20 2011-09-13 Dongguk University Industry-Academic Cooperation Foundation Method for the preparation of dermal papilla tissue employing mesenchymal stem cells
CA2712496C (en) * 2008-01-18 2021-01-12 Wei-Shou Hu Stem cell aggregates and methods for making and using
US20110059182A1 (en) * 2008-02-05 2011-03-10 University Of Virginia Patent Foundation Spraying device and related method for cell aggregates and cell aggregate suspension thereof
US20110165128A1 (en) * 2008-03-07 2011-07-07 Columbia University In The City Of New York Homing in mesenchymal stem cells
GB0805670D0 (en) * 2008-03-28 2008-04-30 Smith & Nephew Increasing the plasticity of stem cells
AU2009281809B2 (en) * 2008-08-14 2015-09-17 Mesoblast International Sarl Purified mesenchymal stem cell compositions and methods of purifying mesenchymal stem cell compositions
US20100112031A1 (en) * 2008-10-17 2010-05-06 University Of Virginia Patent Foundation Compositions And Methods For Regulating Extracellular Matrix Production In Adipose Derived Cells
US20100098739A1 (en) * 2008-10-20 2010-04-22 University Of Virginia Patent Foundation Compositions and methods for modular soft tissue repair
WO2010129775A1 (en) 2009-05-06 2010-11-11 University Of Virginia Patent Foundation Self-illuminated handheld lens for retinal examination and photography and related method thereof
US8883210B1 (en) 2010-05-14 2014-11-11 Musculoskeletal Transplant Foundation Tissue-derived tissuegenic implants, and methods of fabricating and using same
US9352003B1 (en) 2010-05-14 2016-05-31 Musculoskeletal Transplant Foundation Tissue-derived tissuegenic implants, and methods of fabricating and using same
US10130736B1 (en) 2010-05-14 2018-11-20 Musculoskeletal Transplant Foundation Tissue-derived tissuegenic implants, and methods of fabricating and using same
SG10201914007YA (en) 2010-08-24 2020-03-30 Univ Minnesota Non-static suspension culture of cell aggregates
US20120087868A1 (en) 2010-10-08 2012-04-12 Gabriele Todd Nanoparticle-loaded cells
US9234177B2 (en) * 2010-10-18 2016-01-12 Sunshine Life Science & Technology Corp. Human multipotent embryonic stem cell-like progenitor cells
EP2655602B1 (en) * 2010-12-22 2019-01-23 The Administrators of the Tulane Educational Fund Method for identifcation and culture of multipotent mesenchymal stem cells with high proliferation potential
US8834928B1 (en) 2011-05-16 2014-09-16 Musculoskeletal Transplant Foundation Tissue-derived tissugenic implants, and methods of fabricating and using same
EP2765186A4 (en) * 2011-05-31 2015-05-27 Snu R&Db Foundation STRUCTURE FOR TEXTILE GENERATION AND METHOD OF MANUFACTURING THEREOF
AU2012275335B2 (en) 2011-06-29 2017-04-20 Biorestorative Therapies, Inc. Brown fat cell compositions and methods
CA2883374C (en) * 2012-08-29 2016-03-22 Euromi S.A. Apparatus for extracting and re-injecting adipose tissue
AU2014253920B2 (en) 2013-04-19 2019-07-04 Biorestorative Therapies, Inc. Human brown adipose derived stem cells and uses
US20150037436A1 (en) 2013-07-30 2015-02-05 Musculoskeletal Transplant Foundation Acellular soft tissue-derived matrices and methods for preparing same
SG11201707615YA (en) 2013-09-19 2017-10-30 Univ Leland Stanford Junior Methods and compositions for producing hepatocyte-like cells
WO2015199416A1 (ko) * 2014-06-23 2015-12-30 단국대학교 천안캠퍼스 산학협력단 피부 또는 혈관 조직 손상 치료용 약학 조성물 및 이의 이용
EP3297694A1 (en) 2015-05-21 2018-03-28 Musculoskeletal Transplant Foundation Modified demineralized cortical bone fibers
US10912864B2 (en) 2015-07-24 2021-02-09 Musculoskeletal Transplant Foundation Acellular soft tissue-derived matrices and methods for preparing same
US11052175B2 (en) 2015-08-19 2021-07-06 Musculoskeletal Transplant Foundation Cartilage-derived implants and methods of making and using same
EP3359553B1 (en) 2015-10-06 2025-03-26 University Of Virginia Patent Foundation Compositions for treating diabetic retinopathy
US20210355199A1 (en) 2018-06-28 2021-11-18 University Of Virginia Patent Foundation Compositions and methods for detecting and regulating fibronectin-integrin interaction and signaling
US11541101B1 (en) 2018-10-09 2023-01-03 University Of Virginia Patent Foundation LEMD3 antagonizes TGF-beta-driven Smad2/3 transcription in a stiffness-dependent fashion in both the nucleus and cytosol
CN111518751A (zh) * 2019-11-20 2020-08-11 广东先康达生物科技有限公司 一种体外激活脂肪干细胞转化成原软骨细胞的方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004022988A1 (ja) * 2002-09-03 2004-03-18 Seiko Epson Corporation 磁気軸受装置
WO2005114178A1 (de) * 2004-05-21 2005-12-01 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Multizelluläre testsysteme

Family Cites Families (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5486359A (en) * 1990-11-16 1996-01-23 Osiris Therapeutics, Inc. Human mesenchymal stem cells
AU4543193A (en) * 1992-06-22 1994-01-24 Henry E. Young Scar inhibitory factor and use thereof
US5591625A (en) * 1993-11-24 1997-01-07 Case Western Reserve University Transduced mesenchymal stem cells
US5906934A (en) * 1995-03-14 1999-05-25 Morphogen Pharmaceuticals, Inc. Mesenchymal stem cells for cartilage repair
DE69634303T2 (de) 1995-11-16 2006-01-05 Case Western Reserve University, Cleveland Chondrogene in vitro induktion von menschlichen mensenchymalen stammzellen
US6200606B1 (en) * 1996-01-16 2001-03-13 Depuy Orthopaedics, Inc. Isolation of precursor cells from hematopoietic and nonhematopoietic tissues and their use in vivo bone and cartilage regeneration
US5827740A (en) * 1996-07-30 1998-10-27 Osiris Therapeutics, Inc. Adipogenic differentiation of human mesenchymal stem cells
US20030082152A1 (en) 1999-03-10 2003-05-01 Hedrick Marc H. Adipose-derived stem cells and lattices
CN1352696A (zh) 1999-03-10 2002-06-05 匹兹堡大学联邦制高等教育 脂肪来源的干细胞和网格
US6429013B1 (en) * 1999-08-19 2002-08-06 Artecel Science, Inc. Use of adipose tissue-derived stromal cells for chondrocyte differentiation and cartilage repair
EP1261694B1 (en) * 2000-02-26 2008-01-16 Artecel, Inc. Pleuripotent stem cells generated from adipose tissue-derived stromal cells and uses thereof
DE10013223C2 (de) * 2000-03-13 2002-07-18 Co Don Ag Verfahren zur in vitro-Herstellung von dreidimensionalem, vitalem Knorpel- oder Knochengewebe und dessen Verwendung als Transplantationsmaterial
EP1496978A4 (en) 2002-04-03 2006-11-29 Artecel Sciences Inc IMPROVEMENTS IN DIFFERENTIATED ADULT DIFFERENTIATED STEM CELLS DERIVED FROM ADIPOSE TISSUE AND USES THEREOF
KR100534215B1 (ko) 2003-11-11 2005-12-08 (주)히스토스템 냉동 보관된 제대혈로부터 중간엽 줄기세포의 분리 및 배양 방법
JP4499041B2 (ja) * 2003-12-11 2010-07-07 独立行政法人科学技術振興機構 擬微小重力環境下での骨髄細胞を用いた３次元軟骨組織構築方法
US20060045872A1 (en) * 2004-08-25 2006-03-02 Universidad Autonoma De Madrid Ciudad Universitaria de Cantoblanco Use of adipose tissue-derived stromal stem cells in treating fistula
WO2006032054A2 (en) 2004-09-14 2006-03-23 The Trustees Of Columbia University In The City Of New York Differentiation of human mesenchymal stem cells to cardiac progenitor cells that promote cardiac repair
KR100833612B1 (ko) 2004-12-29 2008-05-30 에프씨비파미셀 주식회사 간엽 간세포로부터 분화된 신경세포를 유효성분으로함유하는 신경계 질환 치료용 약학 조성물
WO2006074075A2 (en) * 2004-12-30 2006-07-13 Primegen Biotech, Llc Adipose-derived stem cells for tissue regeneration and wound healing
EP1909581A4 (en) 2005-07-29 2008-12-31 Univ Virginia Adipose tissue stem cells, perivascular cells and pericytes
WO2007030652A2 (en) 2005-09-08 2007-03-15 University Of Virginia Patent Foundation Methods and compositions for growing adipose stem cells
KR100679642B1 (ko) * 2005-11-16 2007-02-06 주식회사 알앤엘바이오 인간 지방조직 유래 다분화능 줄기세포 및 이를 함유하는세포치료제
EP1984488B1 (en) 2006-01-30 2017-12-27 University Of Virginia Patent Foundation Methods of preparing and characterizing mesenchymal stem cell aggregates and uses thereof
US8835170B2 (en) * 2006-10-06 2014-09-16 University Of Virginia Patent Foundation Methods and compositions useful for diabetic wound healing
US7787327B2 (en) 2006-11-15 2010-08-31 Baker Hughes Incorporated Cement bond analysis
US20100098739A1 (en) * 2008-10-20 2010-04-22 University Of Virginia Patent Foundation Compositions and methods for modular soft tissue repair

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004022988A1 (ja) * 2002-09-03 2004-03-18 Seiko Epson Corporation 磁気軸受装置
WO2005114178A1 (de) * 2004-05-21 2005-12-01 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Multizelluläre testsysteme

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
JPN6012028902; Ｂｉｏｔｅｃｈｎｏｌ．Ｂｉｏｅｎｇ Ｖｏｌ．８３，　Ｎｏ．２, 2003, ｐ．１７３-１８０ *
JPN6012028905; Ｅｘｐ．Ｃｅｌｌ　Ｒｅｓ． Ｖｏｌ．２３８, 1998, ｐ．２６５-２７２ *

Cited By (3)

* Cited by examiner, † Cited by third party
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JP2010505849A (ja) * 2006-10-06 2010-02-25 ユニバーシティ オブ バージニア パテント ファウンデーション 糖尿病性創傷治癒に有用な方法および組成物
US8835170B2 (en) 2006-10-06 2014-09-16 University Of Virginia Patent Foundation Methods and compositions useful for diabetic wound healing
JP2012502636A (ja) * 2008-09-22 2012-02-02 ウニヴェルジテート チューリッヒ プロレクトラート エムエヌヴェー 懸滴プレート

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