JP2009247255A - Method for cleaning breeding water and apparatus for cleaning breeding water - Google Patents

Method for cleaning breeding water and apparatus for cleaning breeding water Download PDF

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JP2009247255A
JP2009247255A JP2008097791A JP2008097791A JP2009247255A JP 2009247255 A JP2009247255 A JP 2009247255A JP 2008097791 A JP2008097791 A JP 2008097791A JP 2008097791 A JP2008097791 A JP 2008097791A JP 2009247255 A JP2009247255 A JP 2009247255A
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breeding water
breeding
nitrogen
carbon dioxide
tank
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JP4966905B2 (en
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Masato Mori
正人 森
Yoshiteru Nakano
喜輝 中野
Shunichi Matsuhashi
俊一 松橋
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Taisei Corp
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W10/00Technologies for wastewater treatment
    • Y02W10/10Biological treatment of water, waste water, or sewage

Abstract

<P>PROBLEM TO BE SOLVED: To provide a method for cleaning breeding water, which does not utilize a biodegradable plastic and safely, stably and continuously removes nitrate nitrogen by a simple operation. <P>SOLUTION: The method for cleaning breeding water includes a deoxygenation process for reducing the amount of oxygen dissolved in a certain amount of water contained in breeding water W<SB>2</SB>collected from a breeding water tank T, a carbon dioxide dissolution process for dissolving carbon dioxide in breeding water W<SB>2</SB>, a nitrogen fixation process for passing the breeding water W<SB>2</SB>having passed through the deoxygenation process and the carbon dioxide dissolution process to a nitrogen fixation tank 3A composed of a filter medium assembly and packed with a microorganism carrier 32 and a breeding water supply process for supplying returning breeding water W<SB>3</SB>having passed through the nitrogen fixation process to the breeding water tank T. <P>COPYRIGHT: (C)2010,JPO&INPIT

Description

本発明は、飼育水槽中の飼育水に含まれる硝酸態窒素を除去する方法および装置に関する。   The present invention relates to a method and apparatus for removing nitrate nitrogen contained in breeding water in a breeding aquarium.

飼育水槽中の飼育水に含まれる硝酸態窒素(硝酸イオン)を除去(脱窒)する方法として、例えば、連続多孔質材料で覆われた生分解性プラスチック(炭素源)を飼育水に投入し、生分解性プラスチックの表面に通性嫌気性細菌である脱窒菌を着床・増殖させる方法(特許文献1参照)や、飼育水を循環させる循環ラインに硝化槽と脱窒槽とを並列に設け、硝化槽中の硝化菌(独立栄養細菌)を利用してアンモニア態窒素や亜硝酸態窒素を硝酸態窒素にまで還元(硝化)するとともに、脱窒槽中の脱窒菌(従属栄養細菌)を利用して硝化で発生した硝酸態窒素を窒素にまで還元(脱窒)する方法(特許文献2参照)が知られている。
特開平10−85782号公報 特開2000−126794号公報
As a method of removing (denitrifying) nitrate nitrogen (nitrate ions) contained in the breeding water in the breeding tank, for example, a biodegradable plastic (carbon source) covered with a continuous porous material is introduced into the breeding water. A method of implanting and growing denitrifying bacteria, which are facultative anaerobic bacteria, on the surface of biodegradable plastics (see Patent Document 1) and a nitrification tank and a denitrification tank are provided in parallel in a circulation line for circulating breeding water , Using nitrifying bacteria (autotrophic bacteria) in the nitrification tank to reduce ammonia nitrogen and nitrite nitrogen to nitrate nitrogen (nitrification) and use denitrifying bacteria (heterotrophic bacteria) in the denitrification tank A method of reducing (denitrifying) nitrate nitrogen generated by nitrification to nitrogen is known (see Patent Document 2).
Japanese Patent Laid-Open No. 10-85782 JP 2000-126794 A

特許文献1の飼育水浄化方法では、微生物の働きにより分解された生分解性プラスチックの成分が飼育水槽中に拡散する場合があるので、脱窒菌以外の従属栄養細菌(例えば、病原菌)が増殖する虞がある。また、特許文献1の飼育水浄化方法では、脱窒量の制御が難しいことから、飼育水槽中の硝酸濃度が安定するまでに時間を要する場合がある。さらに、不測の事態により、アンモニア、亜硝酸、硫化水素が、飼育生物に害を生ずる程度まで高まる虞もある。   In the breeding water purification method of Patent Document 1, since biodegradable plastic components decomposed by the action of microorganisms may diffuse into the breeding aquarium, heterotrophic bacteria other than denitrifying bacteria (for example, pathogenic bacteria) grow. There is a fear. Further, in the breeding water purification method of Patent Document 1, since it is difficult to control the amount of denitrification, it may take time until the nitric acid concentration in the breeding water tank is stabilized. In addition, due to unforeseen circumstances, ammonia, nitrous acid, and hydrogen sulfide may increase to such an extent that they cause harm to domestic animals.

特許文献2の飼育水浄化方法では、飼育水を脱窒槽に間歇通水することにより、脱窒槽内を脱窒の起こりやすい嫌気性雰囲気を創出しているが、間歇通水するための機構を具備させると、その制御が煩雑になるとともに、脱窒槽に導入される飼育水の量が制限される虞がある。   In the breeding water purification method of Patent Document 2, an anaerobic atmosphere that easily causes denitrification is created in the denitrification tank by intermittently passing the breeding water through the denitrification tank. If provided, the control becomes complicated and the amount of breeding water introduced into the denitrification tank may be limited.

このような観点から、本発明は、生分解性プラスチックを利用せず、危険性が少なく簡単な操作で安定的・連続的に硝酸態窒素を除去することが可能な飼育水浄化方法および飼育水浄化装置を提供することを課題とする。   From this point of view, the present invention is a breeding water purification method and breeding water that does not use biodegradable plastics and can remove nitrate nitrogen stably and continuously with a simple operation with little danger. It is an object to provide a purification device.

このような課題を解決する本発明に係る飼育水浄化方法は、飼育水槽から取得した飼育水に含まれる溶存酸素量を減少させる脱酸素過程と、飼育水に二酸化炭素を溶解させる二酸化炭素溶解過程と、濾材の集合体からなる微生物担体を充填した窒素固定槽に前記脱酸素過程および前記二酸化炭素溶解過程を経た飼育水を通水する窒素固定過程と、前記窒素固定過程を経た飼育水を前記飼育水槽に戻す飼育水供給過程と、を含むことを特徴とする。   The breeding water purification method according to the present invention that solves such problems includes a deoxygenation process that reduces the amount of dissolved oxygen contained in breeding water obtained from a breeding aquarium, and a carbon dioxide dissolution process that dissolves carbon dioxide in the breeding water. And a nitrogen fixation process for passing the breeding water that has passed through the deoxygenation process and the carbon dioxide dissolution process to a nitrogen fixation tank filled with a microorganism carrier comprising an aggregate of filter media, and the breeding water that has undergone the nitrogen fixation process And a breeding water supply process for returning to the breeding aquarium.

本発明に係る飼育水浄化方法によれば、窒素固定槽内に二酸化炭素を含んだ低溶存酸素雰囲気の飼育水が供給されるようになるので、窒素固定槽内の窒素固定菌(二酸化炭素を炭素源とする通性嫌気性細菌に分類される菌の一部)の活動・増殖が活発になり、硝酸体窒素が窒素固定菌の増殖に消費され、硝酸態窒素が菌体として固定されるので、飼育水中の硝酸態窒素濃度を減少させることが可能になる。しかも、本発明によれば、二酸化炭素を含んだ低溶存酸素雰囲気の飼育水が連続的に窒素固定槽内に供給されるようになるので、安定的・連続的に硝酸態窒素を除去することが可能となり、間歇通水するための装置等も不要になる。   According to the breeding water purification method according to the present invention, breeding water having a low dissolved oxygen atmosphere containing carbon dioxide is supplied into the nitrogen fixing tank. Activity of some of the bacteria classified as facultative anaerobic bacteria used as a carbon source) becomes active, nitrate nitrogen is consumed for growth of nitrogen-fixing bacteria, and nitrate nitrogen is fixed as cells Therefore, it becomes possible to reduce the nitrate nitrogen concentration in the breeding water. Moreover, according to the present invention, breeding water in a low-dissolved oxygen atmosphere containing carbon dioxide is continuously supplied into the nitrogen fixing tank, so that nitrate nitrogen can be removed stably and continuously. This eliminates the need for a device for intermittent water flow.

なお、前記脱酸素過程では、飼育水槽から取得した飼育水に飽和溶存二酸化炭素量以上の二酸化炭素を供給することで、前記飼育水に含まれる溶存酸素量を減少させることもできる。このようにすると、脱酸素過程および二酸化炭素溶解過程が同時並行的に行われることになるので、簡略化した装置で溶存酸素を減少させることが可能となる。   In addition, in the said deoxygenation process, the amount of dissolved oxygen contained in the said breeding water can also be reduced by supplying carbon dioxide more than the amount of saturated dissolved carbon dioxide to the breeding water acquired from the breeding water tank. If it does in this way, since a deoxygenation process and a carbon dioxide melt | dissolution process will be performed simultaneously, it becomes possible to reduce dissolved oxygen with the simplified apparatus.

前記飼育水供給過程では、前記窒素固定過程を経た飼育水に酸素を供給してもよい。このようにすると、飼育水槽に戻される飼育水中の溶存酸素量を増やすことができるので、飼育水槽中の飼育水の水質を良好な状態に維持することが可能になる。なお、酸素を含む混合気体(例えば、大気等)を供給することで飼育水に酸素を供給してもよい。   In the breeding water supply process, oxygen may be supplied to the breeding water that has undergone the nitrogen fixation process. If it does in this way, since the amount of dissolved oxygen in the breeding water returned to the breeding tank can be increased, it becomes possible to maintain the quality of the breeding water in the breeding tank in a good state. Oxygen may be supplied to the breeding water by supplying a mixed gas containing oxygen (for example, the atmosphere).

なお、窒素固定菌は、硝酸態窒素を体内に取り込んで同化するので、飼育水槽中の飼育水から除去された硝酸態窒素の大部分は、窒素固定菌に同化された状態で系内に残留することになる。したがって、何らかの要因によって窒素固定槽内のバランスが崩れると、窒素固定菌に同化されていた窒素化合物が再び飼育水槽内に供給され、飼育水の水質を悪化させる虞がある。このような問題が懸念される場合には、窒素固定槽を逆洗するか、あるいは、前記窒素固定槽から前記微生物担体を取り出し、前記微生物担体に坦持されている窒素固定菌を洗い流すとよい。このようにすると、窒素固定菌に同化された硝酸態窒素が窒素固定菌とともに系外に取り出されることになるので、アンモニアや亜硝酸イオンが再発生するリスクを低減することが可能となる。   Since nitrogen-fixing bacteria take nitrate nitrogen into the body and assimilate, most of the nitrate nitrogen removed from the breeding water in the breeding tank remains in the system in an assimilated state with nitrogen-fixing bacteria. Will do. Therefore, if the balance in the nitrogen fixing tank is lost due to some factor, the nitrogen compound assimilated to the nitrogen fixing bacteria is supplied again into the breeding tank, and the quality of the breeding water may be deteriorated. If such a problem is a concern, the nitrogen fixing tank may be backwashed, or the microbial carrier may be taken out of the nitrogen fixing tank and the nitrogen-fixing bacteria carried on the microbial carrier may be washed away. . In this way, nitrate nitrogen assimilated by the nitrogen-fixing bacteria is taken out of the system together with the nitrogen-fixing bacteria, so that it is possible to reduce the risk that ammonia and nitrite ions are regenerated.

前記した課題を解決する本発明に係る飼育水浄化装置は、飼育水槽中の飼育水を循環させる循環ラインと、前記飼育水槽から取得した飼育水に含まれる溶存酸素量を減少させる脱酸素手段と、前記飼育水に二酸化炭素を溶解させる二酸化炭素溶解手段と、濾材の集合体からなる微生物担体を充填して形成した窒素固定槽と、を備える飼育水浄化装置であって、前記脱酸素手段を、前記窒素固定槽の上流側に設けたことを特徴とする。   The breeding water purification apparatus according to the present invention that solves the above-described problems includes a circulation line for circulating the breeding water in the breeding aquarium, and a deoxygenation means for reducing the amount of dissolved oxygen contained in the breeding water obtained from the breeding tank. A breeding water purification apparatus comprising: a carbon dioxide dissolving means for dissolving carbon dioxide in the breeding water; and a nitrogen fixing tank formed by filling a microorganism carrier comprising an aggregate of filter media, wherein the deoxygenation means comprises: It is provided on the upstream side of the nitrogen fixing tank.

本発明に係る飼育水浄化装置によれば、窒素固定槽内に低溶存酸素雰囲気の飼育水が供給されるようになるので、窒素固定槽内の窒素固定菌の活動・増殖が活発になる。つまり、硝酸態窒素が窒素固定菌に同化されるようになるので、飼育水中の硝酸態窒素濃度を減少させることが可能になる。しかも、本発明によれば、低溶存酸素雰囲気の飼育水が連続的に窒素固定槽内に供給されるようになるので、安定的・連続的に硝酸態窒素を除去することが可能となり、間歇通水するための装置等も不要になる。また、本発明において、微生物担体を砂とし、公知の逆洗装置と組み合わせれば、その洗浄作業を自動的に行うことができる。   According to the breeding water purification apparatus according to the present invention, breeding water having a low dissolved oxygen atmosphere is supplied into the nitrogen fixing tank, so that the activity and proliferation of the nitrogen fixing bacteria in the nitrogen fixing tank becomes active. That is, since nitrate nitrogen is assimilated to nitrogen-fixing bacteria, the nitrate nitrogen concentration in the breeding water can be reduced. Moreover, according to the present invention, breeding water in a low dissolved oxygen atmosphere is continuously supplied into the nitrogen fixing tank, so that nitrate nitrogen can be removed stably and continuously. A device for passing water is also unnecessary. In the present invention, if the microorganism carrier is sand and combined with a known backwash device, the washing operation can be performed automatically.

本発明によれば、生分解性プラスチックや嫌気槽を必要とせず、また、複雑な過程を経ることなく、簡単な操作で安全・安定的・継続的に硝酸態窒素を除去することが可能となる。   According to the present invention, it is possible to remove nitrate nitrogen safely, stably and continuously with a simple operation without requiring a biodegradable plastic or an anaerobic tank and without going through a complicated process. Become.

添付した図面を参照しつつ、本発明を実施するための最良の形態の一例を詳細に説明する。   An example of the best mode for carrying out the present invention will be described in detail with reference to the accompanying drawings.

本実施形態に係る飼育水浄化装置Aは、飼育水槽T中の飼育水W1を循環させる循環ライン1と、この循環ライン1に設けられた水質調整手段2、窒素固定手段3および酸素供給手段4とを備えている。 The breeding water purification apparatus A according to the present embodiment includes a circulation line 1 for circulating the breeding water W 1 in the breeding aquarium T, water quality adjusting means 2, nitrogen fixing means 3 and oxygen supply means provided in the circulation line 1. 4 is provided.

なお、飼育水槽Tには、硝化菌による硝化を行うための硝化手段(循環ラインB1、循環ポンプB2、底面濾過層B3)、タンパク質を除去するタンパク質除去手段(循環ラインC1、循環ポンプC2、プロテインスキマーC3)、飼育水W1の温度調節を行う温度調節手段(温度センサD1、サーモコントローラD2、ヒータD3、クーラーD4)などが付設されている。 The breeding tank T has nitrification means (circulation line B1, circulation pump B2, bottom filtration layer B3) for nitrification by nitrifying bacteria, and protein removal means (circulation line C1, circulation pump C2, protein for removing proteins). skimmer C3), breeding water W temperature adjusting means adjusting the temperature of 1 (temperature sensor D1, thermo controller D2, heater D3, cooler D4) and the like are attached.

循環ライン1は、飼育水槽Tに設けられた取水口11から出水口12に至る流路(配管)であって、水質調整手段2、窒素固定手段3および酸素供給手段4を直列に繋いでいる。なお、循環ライン1には、飼育水W1〜W5を循環させるための循環ポンプ13や流量調節を行うためのバルブ14が介設されている。 The circulation line 1 is a flow path (pipe) from the water intake 11 to the water outlet 12 provided in the breeding water tank T, and connects the water quality adjusting means 2, the nitrogen fixing means 3 and the oxygen supply means 4 in series. . The circulation line 1 is provided with a circulation pump 13 for circulating the breeding water W 1 to W 5 and a valve 14 for adjusting the flow rate.

水質調整手段2は、窒素固定手段3の上流側に設けられるものであって、飼育水槽Tから取得した飼育水W2に含まれる溶存酸素量を減少させる機能と、飼育水W2に二酸化炭素を溶解させる機能(すなわち、飼育水W2中の溶存二酸化炭素量を増加させる機能)とを兼ね備えている。すなわち、本実施形態の水質調整手段2は、飼育水W2に含まれる溶存酸素量を減少させる脱酸素手段として機能するとともに、飼育水W2に二酸化炭素を溶解させる二酸化炭素溶解手段として機能する。 Water conditioning unit 2, there is provided on the upstream side of the nitrogen-fixing means 3, a function of reducing the amount of dissolved oxygen contained in the rearing water W 2 obtained from the breeding aquarium T, carbon dioxide breeding water W 2 And a function of dissolving the water (that is, a function of increasing the amount of dissolved carbon dioxide in the breeding water W 2 ). That is, the water quality adjusting means 2 of this embodiment functions as a deoxygenating means for reducing the amount of dissolved oxygen contained in the breeding water W 2 and also functions as a carbon dioxide dissolving means for dissolving carbon dioxide in the breeding water W 2. .

水質調整手段2の構成に制限はないが、本実施形態のものは、上面が開口した開放容器21と、二酸化炭素を供給する炭酸ガスボンベ(二酸化炭素供給源)22と、この炭酸ガスボンベ22から開放容器21に至る配管23とを備えている。   Although there is no restriction | limiting in the structure of the water quality adjustment means 2, the thing of this embodiment is open | released from the open container 21 which the upper surface opened, the carbon dioxide gas cylinder (carbon dioxide supply source) 22 which supplies a carbon dioxide, and this carbon dioxide gas cylinder 22 And a pipe 23 leading to the container 21.

なお、本実施形態では、開放容器21内における飼育水W2の流れが下向きとなるように、開放容器21の上部に流入口を設け、下部に流出口を設けているが、飼育水W2の流れの向きを限定する趣旨ではない。 In the present embodiment, as the flow of breeding water W 2 in an open container 21 is down, an inlet provided in an upper portion of the open container 21, is provided with the outlet at the bottom, breeding water W 2 It is not intended to limit the direction of flow.

窒素固定手段3は、水質調整手段2の下流側に設けられるものであって、水質調整手段2を通過した飼育水W3に含まれる硝酸態窒素(硝酸イオン)を固定する機能を備えている。本実施形態の窒素固定手段3は、直列に設けられた二つの窒素固定槽3A,3Aからなる。窒素固定槽3Aは、密閉容器31と、この密閉容器31内に充填された微生物担体32とを備えている。 The nitrogen fixing means 3 is provided on the downstream side of the water quality adjusting means 2 and has a function of fixing nitrate nitrogen (nitrate ions) contained in the breeding water W 3 that has passed through the water quality adjusting means 2. . The nitrogen fixing means 3 of this embodiment includes two nitrogen fixing tanks 3A and 3A provided in series. The nitrogen fixing tank 3 </ b> A includes a sealed container 31 and a microorganism carrier 32 filled in the sealed container 31.

密閉容器31内における飼育水W3の流れの向きを限定する趣旨ではないが、本実施形態では、上流側の窒素固定槽3Aにおいては下向きになっており、下流側の窒素固定槽3Aにおいては上向きになっている。すなわち、上流側の窒素固定槽3Aにおいては、密閉容器31の上部に流入口を設けるとともに下部に流出口を設けており、下流側の窒素固定槽3Aにおいては、密閉容器31の下部に流入口を設けるとともに上部に流出口を設けている。 Although not intended to limit the direction of the flow of the breeding water W 3 in the sealed container 31, in the present embodiment, the upstream nitrogen fixing tank 3 </ b> A faces downward, and the downstream nitrogen fixing tank 3 </ b> A It is facing upward. That is, in the upstream nitrogen fixing tank 3A, an inlet is provided in the upper part of the sealed container 31 and an outlet is provided in the lower part. In the downstream nitrogen fixing tank 3A, an inlet is provided in the lower part of the sealed container 31. And an outlet at the top.

微生物担体32は、濾材の集合体からなる。濾材の材質に制限はなく、多孔質ガラス、多孔質セラミック、サンゴ砂、天然樹脂製または合成樹脂製のスポンジ、活性炭などの多孔質材料や、天然岩石やガラスなどの無孔質材料を使用することができる。また、濾材の寸法・形状にも制限はなく、例えば、球状、多面体状、柱状、筒状、リング状、平板状、砂状、砂利状、礫状等の形態に成形されたものを使用することができるが、その内部が嫌気性状態(溶存酸素や結合性酸素がほとんど存在しない状態)にならないように、粒径または肉厚が5mm以下のものを使用することが望ましい。このようにすると、水生生物に対して毒性の強いアンモニア、亜硝酸イオン、硫化水素等の発生を抑制することが可能となる。   The microbial carrier 32 is composed of an aggregate of filter media. There is no restriction on the material of the filter medium, and porous materials such as porous glass, porous ceramic, coral sand, natural resin or synthetic resin sponge, activated carbon, and nonporous materials such as natural rock and glass are used. be able to. Further, the size and shape of the filter medium are not limited, and for example, a filter medium shaped into a spherical shape, a polyhedral shape, a columnar shape, a cylindrical shape, a ring shape, a flat plate shape, a sand shape, a gravel shape, a gravel shape, or the like is used. However, it is desirable to use one having a particle size or thickness of 5 mm or less so that the inside thereof does not enter an anaerobic state (a state in which almost no dissolved oxygen or binding oxygen exists). This makes it possible to suppress the generation of ammonia, nitrite ions, hydrogen sulfide and the like that are highly toxic to aquatic organisms.

酸素供給手段4は、窒素固定手段3の下流側に設けられるものであって、窒素固定手段3を通過した飼育水W4に酸素を溶解させる機能(すなわち、飼育水W4中の溶存酸素量を増加させる機能)を備えている。 The oxygen supply means 4 is provided on the downstream side of the nitrogen fixing means 3 and has a function of dissolving oxygen in the breeding water W 4 that has passed through the nitrogen fixing means 3 (that is, the amount of dissolved oxygen in the breeding water W 4 The function to increase.

酸素供給手段4の構成に制限はないが、本実施形態のものは、上面が開口した開放容器41,41と、大気を送り出すエアブロア(酸素供給源)42と、このエアブロア42から開放容器41,41に至る配管43,43とを備えている。   The configuration of the oxygen supply means 4 is not limited, but in the present embodiment, the open containers 41 and 41 whose upper surfaces are open, the air blower (oxygen supply source) 42 for sending out the atmosphere, and the open containers 41 and 41 from the air blower 42 are provided. And pipes 43 and 43 extending to 41.

なお、本実施形態では、開放容器41内における飼育水W4の流れが下向きとなるように、開放容器41の上部に流入口を設けるとともに下部に流出口を設けているが、飼育水W4の流れの向きを限定する趣旨ではない。 In addition, in this embodiment, although the inflow port is provided in the upper part of the open container 41 and the outflow port is provided in the lower part so that the flow of the breeding water W 4 in the open container 41 may face downward, the breeding water W 4 It is not intended to limit the direction of flow.

エアブロア42は、配管43,43を介して開放容器41,41に大気を供給するものであるが、本実施形態のものは、配管44を介して飼育水槽T中の飼育水Wにも大気(酸素)を供給する。 The air blower 42 supplies air to the open containers 41, 41 via the pipes 43, 43, but the air blower 42 also supplies air to the breeding water W 1 in the breeding water tank T via the pipe 44. (Oxygen) is supplied.

次に、本実施形態に係る飼育水浄化方法を説明する。本実施形態に係る飼育水浄化方法は、脱酸素過程と、二酸化炭素溶解過程と、窒素固定過程と、飼育水供給過程とを備えるものである。なお、飼育水槽T内の飼育水Wは、溶存酸素量が7mg/リットル程度、pHが8〜8.5に維持されているものとする。 Next, the breeding water purification method according to this embodiment will be described. The breeding water purification method according to the present embodiment includes a deoxygenation process, a carbon dioxide dissolution process, a nitrogen fixation process, and a breeding water supply process. Incidentally, breeding water W 1 in the breeding aquarium T shall dissolved oxygen is maintained about 7 mg / liter, pH is in the 8-8.5.

脱酸素過程は、飼育水槽Tから取得した飼育水W2に含まれる溶存酸素量を減少させる過程である。本実施形態では、水質調整手段2を利用して脱酸素過程を行っていて、開放容器21中の飼育水W2に飽和溶存二酸化炭素量以上の二酸化炭素を供給することで、飼育水W2に含まれる溶存酸素量を減少させている。なお、本実施形態の脱酸素過程では、開放容器21の流出口から流出する飼育水W3(脱酸素過程を経た飼育水W3)の溶存酸素量が、3.0mg/リットル程度となるように炭酸ガスボンベ22の放出量を設定している。なお、脱酸素過程を経た飼育水W3の溶存酸素量が脱酸素過程前の飼育水W2の飽和溶存酸素量が少なくなり過ぎると、窒素固定槽3A内の一部分が嫌気性状態になる虞がある(偏性嫌気性細菌が繁殖する虞がある)ので、脱酸素過程を経た飼育水W3の溶存酸素量は、所定値以上であることが望ましい。この所定値は、濾材の種類、充填度合、送水量、水質等によって変化するので、亜硝酸等の発生量を測定しながら下限値を決めることになるが、2.5〜3mg/リットル程度が好ましい。 The deoxygenation process is a process of reducing the amount of dissolved oxygen contained in the breeding water W 2 obtained from the breeding tank T. In the present embodiment, by utilizing the water conditioning unit 2 have been deoxygenated process, the breeding water W 2 in an open container 21 by supplying the saturated dissolved amount of carbon dioxide or carbon dioxide, breeding water W 2 The amount of dissolved oxygen contained in is reduced. Incidentally, as in the deoxygenation process of the present embodiment, the amount of dissolved oxygen in breeding water W 3 flowing out (breeding water W 3 that has passed through the deoxidation process) from the outlet of the open container 21, is about 3.0 mg / l The discharge amount of the carbon dioxide cylinder 22 is set. If the amount of dissolved oxygen in the breeding water W 3 that has undergone the deoxygenation process is too small in the amount of saturated dissolved oxygen in the breeding water W 2 before the deoxygenation process, a portion of the nitrogen fixing tank 3A may become anaerobic. (There is a possibility that obligate anaerobic bacteria may propagate), so it is desirable that the amount of dissolved oxygen in the breeding water W 3 that has undergone the deoxygenation process be a predetermined value or more. This predetermined value varies depending on the type of filter medium, the degree of packing, the amount of water delivered, the water quality, etc., so the lower limit is determined while measuring the amount of nitrous acid generated, etc., but about 2.5 to 3 mg / liter preferable.

二酸化炭素溶解過程は、飼育水槽Tから取得した飼育水W2に二酸化炭素を溶解させる過程である。すなわち、二酸化炭素溶解過程は、飼育水槽Tから取得した飼育水W2の溶存二酸化炭素量を増加させる過程である。本実施形態では、脱酸素過程において二酸化炭素を供給しているので、二酸化炭素溶解過程は、脱酸素過程と同時並行的に行われることになる。二酸化炭素がほぼ飽和状態にある飼育水のpHは、6程度になっている。 The carbon dioxide dissolution process is a process of dissolving carbon dioxide in the breeding water W 2 obtained from the breeding tank T. That is, the carbon dioxide dissolution process is a process of increasing the amount of dissolved carbon dioxide in the breeding water W 2 obtained from the breeding tank T. In the present embodiment, since carbon dioxide is supplied in the deoxygenation process, the carbon dioxide dissolution process is performed in parallel with the deoxygenation process. The pH of breeding water in which carbon dioxide is almost saturated is about 6.

窒素固定過程は、脱酸素過程および二酸化炭素溶解過程を経た飼育水W3を窒素固定槽3Aに通水する過程である。すなわち、窒素固定過程は、二酸化炭素を多く含んだ低溶存酸素雰囲気の飼育水W3を窒素固定槽3A内に供給する過程である。飼育水W3を窒素固定槽3A内に供給すると、窒素固定槽3A内の窒素固定菌(二酸化炭素を炭素源とする通性嫌気性細菌に分類される菌の一部)の活動・増殖(同化型硝酸還元)が活発になる。つまり、硝酸イオンが窒素固定菌の増殖に消費され、硝酸イオンが菌体として固定されるので、飼育水W3中の硝酸イオン濃度が減少することになる。 The nitrogen fixation process is a process in which the breeding water W 3 that has passed through the deoxygenation process and the carbon dioxide dissolution process is passed through the nitrogen fixation tank 3A. That is, the nitrogen fixing process is a process of supplying the breeding water W 3 having a low dissolved oxygen atmosphere containing a large amount of carbon dioxide into the nitrogen fixing tank 3A. When the breeding water W 3 is supplied into the nitrogen fixing tank 3A, the activity / proliferation of nitrogen fixing bacteria in the nitrogen fixing tank 3A (part of bacteria classified as facultative anaerobic bacteria using carbon dioxide as a carbon source) ( Anabolic nitric acid reduction) becomes active. That is, nitrate ions are consumed for the growth of nitrogen-fixing bacteria, and nitrate ions are fixed as cells, so that the nitrate ion concentration in the breeding water W 3 decreases.

飼育水供給過程は、窒素固定過程を経た飼育水W4を飼育水槽Tに戻す過程であるが、本実施形態では、窒素固定過程を経た飼育水W4に大気を供給した後に、飼育水槽Tに戻している。本実施形態では、酸素供給手段4を利用して大気の供給を行っていて、開放容器41中の飼育水W4にエアブロア42から大気を供給することで、飼育水W4の溶存酸素量を増加させるとともに、溶存二酸化炭素量を減少させている。なお、本実施形態では、下流側の開放容器41の流出口から流出する飼育水W5の溶存酸素量およびpHが飼育水槽T中の水生生物に適したものとなるようにエアブロア42の放出量や開放容器41の個数等を設定している。 The breeding water supply process is a process in which the breeding water W 4 that has undergone the nitrogen fixation process is returned to the breeding tank T. In this embodiment, after the atmosphere is supplied to the breeding water W 4 that has undergone the nitrogen fixation process, It has returned to. In the present embodiment, the oxygen supply means 4 is used to supply the atmosphere, and the atmosphere is supplied from the air blower 42 to the breeding water W 4 in the open container 41, so that the dissolved oxygen amount of the breeding water W 4 is reduced. While increasing the amount, the amount of dissolved carbon dioxide is decreased. In this embodiment, the amount of the air blower 42 released so that the dissolved oxygen amount and pH of the breeding water W 5 flowing out from the outlet of the downstream open container 41 are suitable for the aquatic organisms in the breeding water tank T. And the number of open containers 41 are set.

以上説明した本実施形態に係る飼育水浄化装置Aおよび飼育水浄化方法によれば、二酸化炭素を含んだ低溶存酸素雰囲気の飼育水W3が連続的に窒素固定槽3A内に供給されるようになるので、安全・安定的・連続的に硝酸態窒素を除去することが可能となり、間歇通水するための装置等も不要になる。 According to the breeding water purification apparatus A and the breeding water purification method according to the present embodiment described above, the breeding water W 3 containing carbon dioxide and having a low dissolved oxygen atmosphere is continuously supplied into the nitrogen fixing tank 3A. Therefore, it is possible to remove nitrate nitrogen safely, stably and continuously, and an apparatus for intermittent water passing becomes unnecessary.

また、飼育水浄化装置Aを硝化手段やタンパク質除去手段と併用すれば、水換えや水補給の回数を少なくした場合であっても、飼育水槽T中の飼育水W1の硝酸イオン濃度を10ppm程度に維持することが可能になる。 In addition, if the breeding water purification apparatus A is used in combination with nitrification means or protein removal means, the nitrate ion concentration of the breeding water W 1 in the breeding tank T is 10 ppm even if the number of times of water change or water supply is reduced. It becomes possible to maintain to the extent.

また、本実施形態に係る飼育水浄化装置Aによれば、脱酸素過程と二酸化炭素溶解過程とが同時並行的に行われることになるので、簡略化した装置で溶存酸素量を減少させ、さらに、溶存二酸化炭素量を増加させることが可能となる。   Moreover, according to the breeding water purification apparatus A according to the present embodiment, since the deoxygenation process and the carbon dioxide dissolution process are performed simultaneously, the amount of dissolved oxygen is reduced with a simplified apparatus. It becomes possible to increase the amount of dissolved carbon dioxide.

なお、窒素固定槽3A内に生息する窒素固定菌は、硝酸イオンを体内に取り込んで同化するので、飼育水W3から除去された硝酸イオンの大部分は、窒素固定菌に同化された状態で系内に残留することになる。したがって、何らかの要因によって窒素固定槽3A内のバランスが崩れると、窒素固定菌に同化されていた窒素化合物が再び飼育水槽T内に供給され、飼育水W1の水質を悪化させる虞がある。このような問題が懸念される場合には、窒素固定槽3Aを定期的に逆洗するか、あるいは、窒素固定槽3Aから微生物担体32を取り出し、微生物担体32に坦持されている窒素固定菌を定期的に洗い流すとよい。このようにすると、窒素固定菌に同化された硝酸イオンが窒素固定菌とともに系外に取り出されることになるので、アンモニアや亜硝酸イオンが再発生するリスクを低減することが可能となる。 The nitrogen-fixing bacteria living in the nitrogen fixing tank 3A assimilate by taking nitrate ions into the body, so that most of the nitrate ions removed from the breeding water W 3 are assimilated by the nitrogen-fixing bacteria. It will remain in the system. Therefore, the balance of nitrogen fixation tank 3A for some reason is lost, the nitrogen compounds have been assimilated to the nitrogen fixing bacteria is supplied again to the rearing water tank T, there is a possibility to deteriorate the quality of breeding water W 1. When such a problem is concerned, the nitrogen fixing tank 3A is regularly backwashed, or the microbial carrier 32 is taken out from the nitrogen fixing tank 3A, and the nitrogen-fixing bacteria carried on the microbial carrier 32 are used. It is recommended to wash out regularly. In this way, nitrate ions assimilated by the nitrogen-fixing bacteria are taken out of the system together with the nitrogen-fixing bacteria, so that it is possible to reduce the risk that ammonia and nitrite ions are regenerated.

また、本実施形態では、窒素固定過程を経た飼育水W4の溶存酸素量を増加させているので、飼育水槽T中の飼育水W1の水質を良好な状態に維持することが可能になる。 Further, in this embodiment, since the increased amount of dissolved oxygen nitrogen fixation process breeding water W 4 passed through, it is possible to maintain the quality of breeding water W 1 in the breeding aquarium T in good condition .

図1に示す飼育水槽Tの総飼育水量を720リットル、窒素固定槽3Aの容量を30リットル(合計60リットル)、酸素供給手段4の開放容器41の容量を30リットル(合計60リットル)、水生生物を2.5kg(0.5kgの真鯛を5匹)として、本実施形態に係る飼育水浄化方法の効果を確認する実験を行った。表1に、実験条件および実験結果を示す。   The total breeding water volume of the breeding tank T shown in FIG. 1 is 720 liters, the capacity of the nitrogen fixing tank 3A is 30 liters (total of 60 liters), the capacity of the open container 41 of the oxygen supply means 4 is 30 liters (total of 60 liters), Experiments were performed to confirm the effect of the breeding water purification method according to the present embodiment with 2.5 kg of living organisms (5 kg of 0.5 kg of red snapper). Table 1 shows experimental conditions and experimental results.

Figure 2009247255
Figure 2009247255

表1に示すように、窒素固定槽3A及び酸素供給手段4における流量を2.0リットル/分として水質調整手段2に二酸化炭素を供給することなく60日間(1〜60日目)、餌を与え続けながら水生生物を飼育したところ、飼育開始時に9ppmであった硝酸イオン濃度が60ppmまで増加したが、61日目以降、窒素固定槽3A及び酸素供給手段4における流量を1.0リットル/分に下げたところ、硝酸イオン濃度は60ppm前後の値で推移した。68日目に、窒素固定槽3A及び酸素供給手段4における流量を0.5リットル/分に下げるとともに、本実施形態に係る飼育水浄化方法となるように水質調整手段2に二酸化炭素を供給し、二酸化炭素を含んだ低溶存酸素雰囲気の飼育水W3を窒素固定槽3A内に供給したところ、二酸化炭素の供給開始直後から窒素固定槽3A内の溶存酸素濃度が3mg/リットル程度になり、その後、硝酸イオン濃度は徐々に低下した。二酸化炭素の供給を開始してから5日後(72日目)には、硝酸イオン濃度が7〜8ppm程度の一定の範囲にとどまるようになり、その後30日間(73〜102日目)維持された。なお、68日目から102日目までの間、窒素固定槽3Aの入口と出口とで溶存酸素量に差は認められず、2.5〜3mg/リットルであった。上記現象は、(1)窒素固定槽3A及び酸素供給手段4の流量を1.0リットル/分に下げたことにより、密閉容器31に充填されている一部の濾材又は濾材間の隙間で溶存酸素が低下し、通性嫌気性細菌が繁殖し始め、(2)この状態で二酸化炭素を供給したことにより、通性嫌気性細菌に分類される菌のうち、二酸化炭素を炭素源に利用できる菌が急速に繁殖した、とすると説明することができる。 As shown in Table 1, the flow rate in the nitrogen fixing tank 3A and the oxygen supply means 4 is 2.0 liters / minute, and carbon dioxide is not supplied to the water quality adjusting means 2 for 60 days (1st to 60th day). When aquatic organisms were bred while continuing to be fed, the nitrate ion concentration, which was 9 ppm at the start of the bred, increased to 60 ppm. From 61st day, the flow rate in the nitrogen fixing tank 3A and the oxygen supply means 4 was 1.0 liter / min As a result, the nitrate ion concentration remained at a value around 60 ppm. On the 68th day, the flow rate in the nitrogen fixing tank 3A and the oxygen supply means 4 is lowered to 0.5 liter / min, and carbon dioxide is supplied to the water quality adjusting means 2 so as to be the breeding water purification method according to the present embodiment. When the breeding water W 3 containing carbon dioxide and having a low dissolved oxygen atmosphere is supplied into the nitrogen fixing tank 3A, the dissolved oxygen concentration in the nitrogen fixing tank 3A becomes about 3 mg / liter immediately after the start of the supply of carbon dioxide, Thereafter, the nitrate ion concentration gradually decreased. Five days after the start of the supply of carbon dioxide (72nd day), the nitrate ion concentration stayed within a certain range of about 7-8 ppm, and was maintained for 30 days (73-102 days). . In addition, from the 68th day to the 102nd day, there was no difference in the amount of dissolved oxygen between the inlet and the outlet of the nitrogen fixing tank 3A, which was 2.5 to 3 mg / liter. The phenomenon described above is as follows: (1) The flow rate of the nitrogen fixing tank 3A and the oxygen supply means 4 is lowered to 1.0 liter / minute, so that it dissolves in a part of the filter medium filled in the sealed container 31 or a gap between the filter media. Oxygen drops, facultative anaerobic bacteria begin to propagate, (2) Carbon dioxide can be used as a carbon source among the bacteria classified as facultative anaerobic bacteria by supplying carbon dioxide in this state It can be explained that the bacteria propagated rapidly.

なお、この後、二酸化炭素の供給を止め、替わりに窒素ガスを供給し、窒素固定槽3A内の溶存酸素濃度を2.5〜3mg/リットルに保持したところ、飼育水槽T内の硝酸イオン濃度が徐々に上がり始め、窒素ガスに替えてから23日目に30ppmに達し、翌24目には160ppmとなった。この値は本件発明を適用しないで126日間飼育をした場合に達すると予想される濃度(飼育開始から60日間の傾向から推定)とほぼ一致する。この現象は、(1)二酸化炭素が供給されなくなったことにより、通性嫌気性細菌が増殖できなくなり、硝酸イオンが固定化されなくなった、(2)繁殖していた通性嫌気性細菌が栄養不足から死滅し、分解された、とすると説明することができる。   After that, when the supply of carbon dioxide was stopped, nitrogen gas was supplied instead, and the dissolved oxygen concentration in the nitrogen fixing tank 3A was kept at 2.5 to 3 mg / liter, the nitrate ion concentration in the breeding water tank T Gradually began to increase, reaching 30 ppm on the 23rd day after switching to nitrogen gas, and 160 ppm on the next 24th day. This value almost coincides with the concentration expected from the breeding for 126 days without applying the present invention (estimated from the tendency for 60 days from the start of breeding). This phenomenon is due to the fact that (1) facultative anaerobic bacteria can no longer grow and nitrate ions are no longer immobilized due to the loss of carbon dioxide supply. It can be explained that it was killed from the shortage and decomposed.

なお、前記した飼育水浄化方法の手順等は、適宜変更してもよい。
例えば、前記した実施形態においては、脱酸素過程と二酸化炭素溶解過程を同時並行的に行う場合(水質調整手段2が脱酸素手段と二酸化炭素溶解手段とを兼ねている場合)を例示したが、別々に行ってもよい。すなわち、飼育水槽Tから取得した飼育水W2に含まれる溶存酸素量を減少させる脱酸素手段と、飼育水W2に二酸化炭素を溶解させる二酸化炭素溶解手段とを別々に設けてもよい。この場合、二酸化炭素溶解手段は、脱酸素手段の下流側に設け、脱酸素過程を行った後に、二酸化炭素溶解過程を行うことが望ましい。なお、脱酸素過程と二酸化炭素溶解過程を別々に行う場合には、二酸化炭素以外の不活性ガス(例えば、窒素ガス)によるバブリングや真空脱気装置を利用して脱酸素過程を行ってもよい。
In addition, you may change suitably the procedure of the above-mentioned breeding water purification method.
For example, in the above-described embodiment, the case where the deoxygenation process and the carbon dioxide dissolution process are performed in parallel (when the water quality adjusting means 2 serves as both the deoxygenation means and the carbon dioxide dissolution means) is exemplified. It may be done separately. That is, a deoxidizing unit for reducing the dissolved oxygen content in the rearing water W 2 obtained from the breeding aquarium T, and carbon dioxide dissolving means for dissolving carbon dioxide in breeding water W 2 may be provided separately. In this case, it is desirable that the carbon dioxide dissolving means is provided on the downstream side of the deoxygenating means, and the carbon dioxide dissolving process is performed after the deoxygenating process. In addition, when performing a deoxygenation process and a carbon dioxide dissolution process separately, you may perform a deoxygenation process using bubbling by an inert gas (for example, nitrogen gas) other than a carbon dioxide, or a vacuum deaeration apparatus. .

また、前記した飼育水浄化装置の構成を変更してもよい。
例えば、前記した実施形態では、窒素固定槽3Aを二つ備える形態を例示したが、窒素固定槽3Aの個数を限定する趣旨ではない。図示は省略するが、窒素固定槽3Aを一つとしてもよいし、三つ以上としてもよい。なお、前記した実施形態では、複数の窒素固定槽3Aを直列に設けた場合を例示したが、並列に設けても差し支えない。
Moreover, you may change the structure of an above-described breeding water purification apparatus.
For example, in the above-described embodiment, an example in which two nitrogen fixing tanks 3A are provided is illustrated, but this is not intended to limit the number of nitrogen fixing tanks 3A. Although illustration is omitted, the nitrogen fixing tank 3A may be one, or may be three or more. In the above-described embodiment, the case where the plurality of nitrogen fixing tanks 3A are provided in series is illustrated, but may be provided in parallel.

また、前記した実施形態では、開放容器41を二つ備える酸素供給手段4を例示したが、開放容器41の個数を限定する趣旨ではない。図示は省略するが、開放容器41を一つとしてもよいし、三つ以上としてもよい。なお、前記した実施形態では、複数の開放容器41を直列に設けた場合を例示したが、並列に設けても差し支えない。   In the above-described embodiment, the oxygen supply unit 4 including two open containers 41 is illustrated, but the number of the open containers 41 is not limited. Although illustration is omitted, the number of open containers 41 may be one, or three or more. In the above-described embodiment, the case where the plurality of open containers 41 are provided in series is illustrated, but may be provided in parallel.

また、飼育水槽T内で行うエアレーションだけで飼育水W1の溶存酸素量を適切な状態に維持できる場合には、酸素供給手段4を省略してもよい。 Further, when only aeration carried out in the breeding aquarium T can maintain a dissolved oxygen amount of breeding water W 1 to an appropriate state may be omitted oxygen supply means 4.

本発明の一実施形態に係る飼育水浄化装置の構成を説明するための循環ろ過系統図である。It is a circulation filtration system diagram for demonstrating the structure of the breeding water purification apparatus which concerns on one Embodiment of this invention.

符号の説明Explanation of symbols

1 循環ライン
2 水質調整手段(脱酸素手段、二酸化炭素溶解手段)
3 窒素固定手段
3A 窒素固定槽
4 酸素供給手段
T 飼育水槽
1〜W5 飼育水
1 Circulation line 2 Water quality adjustment means (deoxygenation means, carbon dioxide dissolution means)
3 nitrogen fixation means 3A nitrogen-fixing tank 4 oxygen supply means T breeding aquarium W 1 to W-5 breeding water

Claims (6)

飼育水槽から取得した飼育水に含まれる溶存酸素量を減少させる脱酸素過程と、
飼育水に二酸化炭素を溶解させる二酸化炭素溶解過程と、
濾材の集合体からなる微生物担体を充填した窒素固定槽に前記脱酸素過程および前記二酸化炭素溶解過程を経た飼育水を通水する窒素固定過程と、
前記窒素固定過程を経た飼育水を前記飼育水槽に戻す飼育水供給過程と、を含むことを特徴とする飼育水浄化方法。
A deoxygenation process that reduces the amount of dissolved oxygen contained in the breeding water obtained from the breeding tank;
Carbon dioxide dissolution process to dissolve carbon dioxide in breeding water,
A nitrogen fixation process of passing the breeding water that has passed through the deoxygenation process and the carbon dioxide dissolution process into a nitrogen fixation tank filled with a microorganism carrier comprising an aggregate of filter media;
A breeding water purification method comprising a breeding water supply process of returning breeding water that has undergone the nitrogen fixation process to the breeding tank.
前記脱酸素過程では、飼育水槽から取得した飼育水に飽和溶存二酸化炭素量以上の二酸化炭素を供給することで、前記飼育水に含まれる溶存酸素量を減少させることを特徴とする請求項1に記載の飼育水浄化方法。   In the deoxygenation process, the amount of dissolved oxygen contained in the breeding water is reduced by supplying carbon dioxide more than a saturated dissolved carbon dioxide amount to breeding water obtained from the breeding aquarium. The breeding water purification method as described. 前記飼育水供給過程において、前記窒素固定過程を経た飼育水に酸素を供給することを特徴とする請求項2に記載の飼育水浄化方法。   3. The breeding water purification method according to claim 2, wherein in the breeding water supply process, oxygen is supplied to the breeding water that has undergone the nitrogen fixation process. 前記窒素固定槽を逆洗する洗浄過程を含むことを特徴とする請求項3に記載の飼育水浄化方法。   4. The breeding water purification method according to claim 3, further comprising a washing process of back washing the nitrogen fixing tank. 前記窒素固定槽から前記微生物担体を取り出し、前記微生物担体に坦持されている窒素固定菌を洗い流す洗浄過程を含むことを特徴とする請求項3に記載の飼育水浄化方法。   4. The breeding water purification method according to claim 3, further comprising a washing step of taking out the microorganism carrier from the nitrogen fixing tank and washing away the nitrogen-fixing bacteria carried on the microorganism carrier. 飼育水槽中の飼育水を循環させる循環ラインと、
前記飼育水槽から取得した飼育水に含まれる溶存酸素量を減少させる脱酸素手段と、
前記飼育水に二酸化炭素を溶解させる二酸化炭素溶解手段と、
濾材の集合体からなる微生物担体を充填して形成した窒素固定槽と、を備える飼育水浄化装置であって、
前記脱酸素手段を、前記窒素固定槽の上流側に設けたことを特徴とする飼育水浄化装置。
A circulation line for circulating the breeding water in the breeding tank,
Deoxygenation means for reducing the amount of dissolved oxygen contained in the breeding water obtained from the breeding tank;
Carbon dioxide dissolving means for dissolving carbon dioxide in the breeding water;
A breeding water purification apparatus comprising: a nitrogen fixing tank formed by filling a microbial carrier composed of an aggregate of filter media,
A breeding water purification apparatus characterized in that the deoxygenation means is provided upstream of the nitrogen fixing tank.
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