JP2009184960A - Skin bleaching cosmetic - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To obtain a cosmetic that acts more positively than conventional ones for the purpose of preventing stains and freckles and producing the white and beautiful skin which a conventional cosmetic can not satisfy. <P>SOLUTION: The improvement in acid phosphatase activity is considered to be effective, and the effect on the improvement of acid phosphatase activity for wide and various substances have been studied. The result showed that, the bleaching cosmetic including an acid phosphatase activity improver containing one or more kinds of compounds selected from a DNA synthesis promoter, a protein synthesis promoter, a pH adjuster, a metal, a plant extract and a microorganism extract and a melanin production inhibitor has an extremely high skin bleaching effect, and has lead to this invention. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

The present invention relates to a whitening cosmetic characterized in that a cosmetic having an acid phosphatase activity improving action and a melanin production-suppressing cosmetic are used in combination.

A cosmetic comprising an acid phosphatase activity improving effect and a melanin production-suppressing cosmetic filled in a container that does not contact each other and mixed at the time of use.

In order to prevent or improve skin darkening due to ultraviolet rays and skin pigmentation such as spots and freckles, ingredients that have been effective in inhibiting melanin production or reducing the produced melanin pigment have been used in whitening cosmetics. Has been blended. Among them, ascorbic acid and its derivatives are widely used because they are biological components and have high safety.

However, ascorbic acid is susceptible to redox reactions and is unstable. Therefore, although an ascorbic acid derivative having improved stability by blending is used, there is a problem that a sufficient whitening effect cannot be exhibited unless the ascorbic acid derivative is hydrolyzed to generate free ascorbic acid.

In addition, phosphate ascorbyl phosphate ascorbic acid derivatives are stable in the neutral to weakly alkaline region, but are not subject to the conditions of acid phosphatase in the skin at this pH range, and therefore are not in a condition to produce free ascorbic acid. . Therefore, it did not exhibit a sufficient effect as a whitening cosmetic.

The problem of the present invention is to solve the above-mentioned problems, and to produce free ascorbic acid by using an acid phosphatase from a phosphate ester ascorbic acid derivative, thereby protecting skin pigmentation such as spots and freckles. It relates to cosmetics that satisfy moisture and produce white and beautiful skin. The present invention relates to a cosmetic that acts more positively than before for the purpose of preventing spots and freckles that could not be satisfied with conventional cosmetics and producing white and beautiful skin.

As a result of intensive studies in view of the above circumstances, the present invention considers that it is effective to improve acid phosphatase activity, and as a result of examining acid phosphatase activity improving action for various substances, DNA synthesis promoter, protein By including an acid phosphatase activity improver and a melanin production inhibitor containing one or more compounds selected from synthesis promoters, pH adjusters, metals, plant extracts, and microbial extracts, It has been found that it has an extremely high whitening effect and has led to the completion of the present invention.

In addition, the ascorbic acid derivative of the melanin production inhibitor is filled in a container that does not come into contact with the acid phosphatase activity improver and mixed at the time of use. Furthermore, by using a plurality of melanin production inhibitors in combination, it is possible to provide a whitening cosmetic that complements the time loss until ascorbic acid is released from the ascorbic acid derivative and has a stronger whitening effect.

As described in detail above, the present invention has an extremely high whitening effect and a high safety by blending an acid phosphatase activity improver and a melanin production inhibitor, and thus can be widely applied to cosmetics.

The compounds having an action to improve the activity of acid phosphatase used in the present invention are compounds that enhance the activity of acid phosphatase in the skin and enhance the activity, compounds that make the pH range suitable for enhancing the activity of acid phosphatase, and compounds that enhance the activity of acid phosphatase. Enzymes and compounds that express acid phosphatase activity can be utilized.

Citric acid, phosphoric acid, amino acids, and salts thereof can be used as the pH adjusting agent for increasing the acid phosphatase activity used in the present invention, and any kind can be used as long as it is a compound capable of adjusting pH. In addition, in order to increase the acid phosphatase activity, it is necessary to adjust the pH in the optimum pH range from 2.0 to 7.0, preferably from 3.0 to 6.0. This is effective for the whitening effect.

The plant extract for increasing acid phosphatase activity or expressing acid phosphatase activity used in the present invention is rice bran extract, rice fermentation extract, rice fermentation extract, potato extract, green mandarin extract, soy milk lactic acid bacteria Examples include fermented extracts and perilla flower spike extracts.
Examples of the microorganism extract include koji mold extract, yeast extract, lactic acid bacteria extract, Bacillus subtilis extract, and the like.

The ascorbic acid derivative that is a melanin production inhibitor used in the present invention is phosphate ascorbate, but the active ingredient is ascorbic acid released in the skin. This ascorbic acid expresses a whitening effect by a reducing action, but since the mechanisms of melanin production are diverse, a further whitening action can be obtained by using a plurality of whitening agents in combination. The whitening agent that can be used in combination is selected from licorice extract, glabrizine, arbutin, kojic acid, ellagic acid, placental extract, hydroquinone, and Sorakuhi extract.

The compounding quantity of the acid phosphatase activity improver in this invention is 0.0001-20.0 weight%, More preferably, it is the range of 0.01-10.0 weight%.

  The compounding quantity of the phosphate ester ascorbate in this invention is 1.0-20.0 weight%, More preferably, it is the range of 3.0-9.0 weight%. If the blending amount is less than 1.0% by weight, the effect of the present invention is poor, and even if it exceeds 20.0% by weight, an effect commensurate with the blending amount cannot be expected.

In addition to the above essential ingredients, the cosmetics of the present invention are aqueous ingredients, plant extracts, animal extracts, powders, excipients, surfactants, oils, alcohols, preservatives, antioxidants, thickeners, sweeteners. It is prepared by appropriately blending agents, pigments, fragrances and the like as necessary. The dosage form of the cosmetic of the present invention is not particularly limited,
Provides various dosage forms such as facial cleansers, lotions, cosmetic liquids, emulsions, creams, gels, packs, aerosol cosmetics, skin care cosmetics such as haps, makeup cosmetics such as foundation creams and foundations, and bath preparations. be able to.

  EXAMPLES Hereinafter, although an Example demonstrates the structure of this invention in detail, this invention is not limited by this. Prior to the examples, the tests performed in each example will be described.

(1) Acid phosphatase activity [reagent]
・ 6% ascorbyl magnesium phosphate solution (pH 7.0)
・ Citrate buffer (pH3.0)
・ Sample solution (diluted each extract with purified water to 10% concentration)
[Measurement]
Mix 2 mL of 6% ascorbyl magnesium phosphate solution with 1 mL of citrate buffer and 1 mL of sample solution and leave for 1 hour. Then, ascorbyl magnesium phosphate was quantified by HPLC, and the amount of free ascorbic acid was calculated from the obtained value.
〔Measurement condition〕
・ Column: Shimpack CLC-ODS (M)
・ Column temperature: 40 ℃
Mobile phase: 0.08M acetic acid-sodium acetate buffer (pH 5.0) containing 2.8 mM n-hexylamine, 0.1 mM disodium edetate and 2% methanol
・ Flow rate: 0.8mL / min

Each extract exhibited an acid phosphatase activity and released ascorbic acid from ascorbyl magnesium phosphate. (table 1).

(2) Acid phosphatase activity improving action [reagent]
・ 6% ascorbyl magnesium phosphate solution (pH 7.0)
・ Sample solution (diluted with purified water to 10% concentration)
[Measurement]
The sample solution is dropped onto the reconstituted three-dimensional cultured skin and cultured for 3 days. After culturing, 0.5 mL of 3% ascorbyl magnesium phosphate solution is dropped and cultured for 24 hours. After culturing, the cultured skin was homogenized to extract magnesium ascorbyl phosphate, and the residual amount was measured by HPLC.

Each extract improved skin cell acid phosphatase activity. (Table 2)

(3) Pigmentation-improving action Ten subjects with pigmentation such as spots and freckles were grouped into one group.
The cosmetics of Examples 1 to 3 were applied to the pigmentation part twice in the morning and evening. Based on these criteria, the pigmentation improving effect was evaluated. The determination was made one month after the start of cosmetic use. No one complained of skin abnormalities throughout the study period.

  The cosmetics of the examples showed a tendency to improve pigmentation such as spots and buckwheat. (Table 4).

  Examples of cosmetics according to the present invention and examples of effect tests are shown below. In addition, the cosmetics of this invention are not restricted to the Example hung up below.

[Production Example 1]
Preparation of fermented rice bran extract Add 10 times the amount of water to rice bran 1. Neisseria gonorrhoeae and yeast were added thereto and left at room temperature for 10 days, followed by filtration to obtain an extract.

[Production Example 2]
Preparation of Fermented Soymilk Lactic Acid Bacteria The lactic acid bacteria were added to soymilk produced by a conventional method and left at room temperature for 7 days, followed by filtration to obtain an extract.

[Production Example 3]
Preparation of potato extract Add 5 times the amount of water to potato 1 and grind it with a mixer. After leaving for 2 hours, filtration was performed to obtain an extract.

[Production Example 4]
Preparation of blue mandarin orange extract After immersing in blue mandarin orange 1 having a diameter of 3 cm or less in 5-fold amount of 30% 1,3-butylene glycol solution at room temperature for 7 days, filtration treatment was performed to obtain an extract.

[Production Example 5]
Preparation of perilla flower spike extract After dipping in perilla flower ear 1 in 5 times amount of 30% 1,3 butylene glycol solution at room temperature for 7 days, filtration treatment was performed to obtain an extract.

[Lotion A]
Ingredient Weight%
Rice bran fermentation extract of Production Example 1 5.000
Fermented extract of soy milk lactic acid bacteria of Production Example 2 5.00
Potato extract from Production Example 3 5.000
Island carrot extract 1.000
Pine bark extract 1.000
Glaze extract 1.000
Oil-soluble licorice extract 1.000
Hesperidin 1.000
α-Arbutin 3000
Platinum colloid solution 0.500
Citric acid 1.000
Glycolic acid 1.000
Glycerin 2.000
1,3-butylene glycol 2.000
Preservative Appropriate amount Purified water The total amount is 100.000

[Lotion B]
Ingredient Weight%
Ascorbyl magnesium phosphate 5.000
Ascorbyl sodium phosphate 1.000
Pentylene glycol 5.000
Citric acid 1.000
Lactic acid 1.000
Dipotassium glycyrrhizinate 0.010
Sodium hydroxide appropriate amount (adjusted to pH 7.2)
Preservative Appropriate amount Purified water Amount that makes the total amount 100.000

[Cosmetic A]
Ingredient Weight%
Blue mandarin extract of Production Example 4 5.00
Perilla flower extract of Production Example 5 5.00 Liquorice extract
1.000
Sohakuhi extract 1.000
Glaze extract 1.000
Grab lysine 0.100
Hesperidin 1.000
β-Arbutin 3000
Platinum colloid solution 0.500
Citric acid 0.500
Lactic acid 0.500
Glycolic acid 2.000
Glycerin 2.000
1,3-butylene glycol 2.000
Xanthan gum 0.400
Preservative Appropriate amount Purified water The total amount is 100.000

[Serum B]
Ingredient Weight%
Ascorbyl magnesium phosphate 5.000
Ascorbyl sodium phosphate 5.000
Pentylene glycol 5.000
Citric acid 1.000
Lactic acid 1.000
Dipotassium glycyrrhizinate 0.010
Xanthan gum 0.400 Sodium hydroxide
Appropriate amount (adjusted to pH 7.2)
Preservative Appropriate amount Purified water The total amount is 100.000

[Cosmetic liquid]
Ingredient Weight%
Rice bran fermentation extract of Production Example 1 5.000
Fermented extract of soy milk lactic acid bacteria of Production Example 2 5.00
Potato extract from Production Example 3 5.000
Blue mandarin extract of Production Example 4 5.00
Perilla flower extract from Production Example 5 1.000
Island carrot extract 1.000
Pine bark extract 1.000
Glaze extract 1.000
Oil-soluble licorice extract 1.000
Hesperidin 1.000
β-Arbutin 3000
Platinum colloid solution 0.500
Citric acid 1.000
Glycolic acid 1.000
Glycerin 2.000
1,3-butylene glycol 2.000
Xanthan gum 0.400
Insoluble capsule-type ascorbyl magnesium phosphate 6.00
Preservative Appropriate amount Purified water The total amount is 100.000

"Comparative Example 1"
Comparison lotion A
Prepared using purified water instead of the fermented rice bran extract, soy milk lactic acid bacteria fermentation extract, potato extract, citric acid, and glycolic acid of Example 1.
Comparison lotion B
Prepared in the same manner as in Example 1.

"Comparative Example 2"
Comparative serum A
It was prepared by using purified water instead of blue mandarin extract, perilla flower spike extract, citric acid, lactic acid and glycolic acid of Example 2.
Comparison lotion B
Prepared in the same manner as in Example 2.

“Comparative Example 3”
Comparison serum 3
It was prepared using purified rice instead of fermented rice bran extract, soy milk lactic acid bacteria fermented extract, potato extract, green mandarin orange extract, perilla spikelet extract, citric acid, and glycolic acid of Example 3.

Claims (4)

  A whitening cosmetic comprising a cosmetic containing a phosphatase, particularly an acid phosphatase activity promoter, and a cosmetic containing a melanin production inhibitor.   2. The acid phosphatase activity promoter according to claim 1, wherein the acid phosphatase activity promoter comprises one or more compounds selected from a DNA synthesis promoter, a protein synthesis promoter, and a pH adjuster. The melanin acid inhibitor according to claim 1, phosphate ascorbate, licorice extract,
A melanin acid inhibitor containing one or more compounds selected from glabridine, α-arbutin, kojic acid, ellagic acid, placental extract, and hydroquinone. 2. A cosmetic whitening cosmetic, wherein the cosmetic containing the acid phosphatase activity promoter according to claim 1 and the melanin production inhibitor are filled in a container that does not contact each other and mixed at the time of use.