JP2009122356A5

JP2009122356A5 -

Info

Publication number: JP2009122356A5
Application number: JP2007295641A
Authority: JP
Filing date: 2007-11-14
Publication date: 2012-04-12

Description

本発明の位相差顕微鏡は、観察標本を載置するステージと、照明光を前記ステージ上に載置された前記観察標本に導く照明光学系と、対物レンズを含み前記観察標本を透過した光を集光する観察光学系と、前記対物レンズの前記観察光学系にある瞳位置に設けられた位相板と、前記対物レンズと共役な位置に設けられ、リング状の光が射出される光学素子とを備えた位相差顕微鏡であって、前記光学素子または前記位相板の何れか一方を移動させる移動手段と、所定の観察位置における前記光学素子からの光が前記位相板に入射した状態における前記光学素子または前記位相板の位置と各前記観察位置との対応関係を予め記録する記録手段と、前記記録手段に記録された前記対応関係に基づいて前記移動手段の観察位置への移動を制御する制御部とを有する。 The phase contrast microscope of the present invention includes a stage for placing an observation specimen, an illumination optical system for guiding illumination light to the observation specimen placed on the stage, and light transmitted through the observation specimen including an objective lens. A condensing observation optical system, a phase plate provided at a pupil position in the observation optical system of the objective lens, an optical element provided at a position conjugate with the objective lens and emitting ring-shaped light A phase difference microscope comprising: a moving means for moving either the optical element or the phase plate; and the optical in a state where light from the optical element at a predetermined observation position is incident on the phase plate. A recording unit that records in advance the correspondence between the position of the element or the phase plate and each observation position; and a control that controls the movement of the moving unit to the observation position based on the correspondence recorded in the recording unit. And a part.

Claims

A stage on which an observation specimen is placed;
An illumination optical system for guiding illumination light to the observation specimen placed on the stage;
An observation optical system that includes an objective lens and collects light transmitted through the observation specimen;
A phase plate provided at a pupil position in the observation optical system of the objective lens;
An optical element provided at a position conjugate with the objective lens, and emitting ring-shaped light;
In a phase contrast microscope equipped with
Moving means for moving either the optical element or the phase plate;
A recording unit that records in advance the correspondence between the position of the optical element or the phase plate and the observation position when light from the optical element at a predetermined observation position is incident on the phase plate;
A control unit that controls movement of the moving unit to the observation position based on the correspondence relationship recorded in the recording unit;
A phase-contrast microscope characterized by comprising:

The information recorded by the recording means is ring position information for one container among a plurality of containers for accommodating the observation specimen ,
When observing the observation specimen stored in another container , the control unit drives the moving unit by using the record for the one container recorded in the recording unit.
The phase contrast microscope according to claim 1 .

The ring position information is a position when either one of the optical element or the phase plate is moved and the contrast of the sample image captured by the imaging device is maximized.
The phase-contrast microscope according to claim 2.

The optical element is a phase ring in which a slit is formed.
The phase-contrast microscope according to any one of claims 1 to 3, wherein the phase-contrast microscope is provided.