CN103529542A - Polarized-light modulation phase contrast microscope - Google Patents
Polarized-light modulation phase contrast microscope Download PDFInfo
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- CN103529542A CN103529542A CN201310512373.2A CN201310512373A CN103529542A CN 103529542 A CN103529542 A CN 103529542A CN 201310512373 A CN201310512373 A CN 201310512373A CN 103529542 A CN103529542 A CN 103529542A
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Abstract
The invention relates to a polarized-light modulation phase contrast microscope. The polarized-light modulation phase contrast microscope comprises an illuminator, a stage and an eyepiece. The polarized-light modulation phase contrast microscope is characterized by further comprising an objective group and a condenser group, wherein the objective group is located below the stage and comprises a plurality of modulation phase contrast objectives with consistent rear focal planes, and each modulation phase contrast objective comprises an objective modulator arranged on the consistent rear focal plane of each modulation phase contrast objective; and the condenser group is located between the illuminator and the stage and comprises a polarizer, a polarization slit converter, a condenser aperture diaphragm and a condenser which are arranged sequentially from top to bottom, and a plurality of polarization slit aperture diaphragms corresponding to modulation phase contrast objectives are arranged on the polarization slit converter. According to the polarized-light modulation phase contrast microscope, a modulation phase contrast observation device is added to a microscopic illumination and imaging system, so that observed microscopic images are high in stereoscopic sensation, the resolution of microscopic observation is improved, meanwhile, the problem of double images due to the fact that the modulator is located in the middle of an objective lens group is also solved, and the microscopic imaging contrast is further increased.
Description
Technical field:
The present invention relates to microscopy field, be specifically related to polarized light modulation phase contrast microexamination technology, in microscope and imaging system, increase modulation phase contrast finder, make the micro-image of observing there is obvious contrast contrast, present the image that stereoscopic sensation is stronger, to improve the resolving power of microexamination, be particularly suitable for the transparent medium surface morphologic observation of no dyeing.
Background technology:
As everyone knows, light beam modulation can be divided into amplitude modulation, frequency modulation, phase modulation and intensity modulated etc. by the character of its modulation.Existing phasecontrast microscope technology mainly comprises phase place phase contrast microscopy, differential interference contrast microscopy, Huffman modulation phase contrast microscopy etc.Wherein phase place phase contrast microscopy refers to that the actual change due to refractive index affects the variation of detecting light beam phase place, again the phase place change transitions of light beam is become to amplitude variations, to show as image contrast difference, when the main advantage of phase contrast microscopy is that living cells is observed, do not need to carry out the processes such as death, sealing and dyeing, can under state of nature, carry out microexamination.Differential interference phase-contrast microtechnic is to utilize the beam splitting of polarized light in uniaxial crystal communication process and compound, thereby produce small path difference, and interfere in objective lens ', by carrying out microexamination after analyzing, the image of observing presents plastic relief sense, and differential interference contrast microscopy can be divided into transmission-type and fall to penetrating two kinds of formulas.Huffman modulation phase contrast microscopy is that the phase gradient of light is converted to intensity variation, can form a high contrast, high-resolution picture to common invisible phase object like this, and show with 3 D stereo state.First two phase contrast microscopy is used widely, and Huffman modulation phase contrast microscopy is this technology of being invented in 1975 by Robert's Huffman doctor, be mainly used in the product of modulated optical company of its establishment, Japan OLYMPUS, NIKON two companies have also introduced relevant micro-imaging technique in the inverted microscope of its manufacture.There are not Related product technical report in optical microscope and institute at home at present.
At present external Huffman modulation phase contrast Microexamination device is mainly to undergo technological transformation on traditional product basis, owing to being subject to the restriction of original design, the back focal plane of object lens is inconsistent often, thereby the clear aperture of the object lens modulator installation site of (also claiming amplitude filter), modulation areas can not be completely unified, need special band polarization slit aperture diaphragm condenser to mate one by one with object lens simultaneously, thereby the price of the Huffman of import modulation phase contrast Microexamination device is very expensive, needs specialty manufacture.
Summary of the invention:
The object of the invention is to overcome the deficiencies in the prior art, a kind of polarized light modulation phasecontrast microscope is provided, it is based on Huffman modulation phase contrast principle, introduce polarized light light intensity modulation technology, by thering is the modulation phase contrast objective lens of consistent back focal plane, be located at the object lens modulator of modulation phase contrast objective lens back focal plane and with the setting of the slit aperture diaphragm condenser group of polarizer, realized the variation that shows as luminance brightness by thering is light beam after the phase place sample of graded, also overcome because modulator is in the middle problem that occurs double image of objective lens group simultaneously, further improve the contrast of micro-imaging.
In order to solve the problems of the technologies described above, the object of the invention is to be achieved through the following technical solutions:
A kind of polarized light modulation phasecontrast microscope, comprise luminaire, objective table and eyepiece, its feature is in also comprising: objective lens and condenser group, described objective lens is positioned at objective table below, comprise that several have the modulation phase contrast objective lens of consistance back focal plane, modulation phase contrast objective lens comprises object lens modulator, and described object lens modulator is arranged on the consistance back focal plane of described each modulation phase contrast objective lens; Described condenser group is between luminaire and objective table, comprise order arranges the polarizer, polarization slit converter, condenser aperture diaphragm and condenser from top to bottom, described polarization slit converter is provided with several polarization slit aperture diaphragms corresponding with each modulation phase contrast objective lens.Further, described polarization slit converter is drawplate type conversion seat, is also provided with the transmission through hole of observing for bright field on described polarization slit converter.
Further, described polarization slit converter is drawplate type converter or rotating disc type converter, is also provided with the transmission through hole of observing for bright field on described polarization slit converter.
Further, described condenser group also comprises the colour filter bar that is arranged at polarizer top.
Further, described objective lens also comprises bright field viewing objective.
As a kind of embodiment, several modulation phase contrast objective lens are respectively that 10X, 20X, 40X modulate phase contrast objective lens, and on polarization slit converter, several polarization slit aperture diaphragms are respectively 10X, 20X, 40X polarization slit aperture diaphragm.
Further, described several polarization slit aperture diaphragms are positioned at the front focal plane of described condenser, in polarization slit aperture diaphragm, are divided into Liang Ge district, are respectively complete transmission area and polarisation intensification modulation district.
Further, described eyepiece is binocular vision mirror, is hinge type structure.
Further, described polarized light modulation phasecontrast microscope also comprises for regulating objective lens with respect to the rough micro-moving mechanism focus adjusting mechanism of lifting of object loading table.
Further, described rough micro-moving mechanism focus adjusting mechanism comprises coarse motion slack adjuster and stop means.
Further again, described polarized light modulation phasecontrast microscope also comprises the photography and vedio recording output port of optics output while gathering for MIcrosope image.
Compared with prior art, polarized light modulation phasecontrast microscope of the present invention has following beneficial effect:
1, the present invention is on the basis of Huffman modulation phase contrast principle, designed and developed the microcobjective that a set of objective lens ' is consistent, be respectively 10X, 20X, 40X, common modulation device is installed on its back focal plane, again according to different multiplying object lens, design is with the polarization slit aperture diaphragm of polarizer, by the rotation of the polarizer, carry out the polarization intensity of modulated beam of light, thereby realize the variation that shows as luminance brightness by thering is light beam after the phase place sample of graded, also overcome because modulator is in the middle problem that occurs double image of objective lens group simultaneously, further improve the contrast of micro-imaging.
2, by the present invention, can effectively improve imaging resolution, reduce production costs, to reach the object of complete substituting import one equipment.
3, polarized light modulation phasecontrast microscope of the present invention can be applicable in biology and fluorescent microscope, can carry out the microexamination of normal bright field, also can modulate phase contrast microexamination, two kinds of observing patterns can be switched fast, simple in structure, easy to operate, can obtain that visual field is even, the obvious micro-image of contrast.
Accompanying drawing explanation
Fig. 1 is polarized light modulation phasecontrast microscope (drawplate type) the one-piece construction schematic diagram of embodiment 1;
Fig. 2 is the schematic top plan view of the condenser group (drawplate type) of embodiment 1;
Fig. 3 is the front schematic view of the condenser group (drawplate type) of embodiment 1;
Fig. 4 is territory, the polarization slit aperture diaphragm transparent zone schematic diagram of the condenser group (drawplate type) of embodiment 1;
Fig. 5 is modulation phase contrast objective lens structural representation of the present invention;
Fig. 6 is territory, the modulation sheet transparent zone schematic diagram in modulator on modulation phase contrast objective lens back focal plane of the present invention;
Fig. 7 is polarized light modulation phasecontrast microscope (rotating disc type) the one-piece construction schematic diagram of embodiment 2;
Fig. 8 is condenser group (rotating disc type) schematic top plan view of embodiment 2;
Fig. 9 is that the A-A of Fig. 8 is to diagrammatic cross-section.
Wherein: 1-luminaire, 2-condenser group, 21-polarization slit converter, 22-polarization slit aperture diaphragm, 23-colour filter bar, the 24-polarizer, 25-condenser aperture diaphragm, 26-condenser, 3-objective table, 4-eyepiece, 5-rough micro-moving mechanism focus adjusting mechanism, 6-objective lens, 61-object lens modulator, 7-photography and vedio recording output port.
Embodiment
Below in conjunction with accompanying drawing, the preferred embodiments of the present invention are described, should be appreciated that preferred embodiment described herein, only for description and interpretation the present invention, is not intended to limit the present invention.
Embodiment 1
Drawplate type polarized light modulation phasecontrast microscope
As shown in Fig. 1～6, a kind of polarized light modulation phasecontrast microscope, comprises luminaire 1, condenser group 2, objective table 3, eyepiece 4, rough micro-moving mechanism focus adjusting mechanism 5, objective lens 6, photography and vedio recording output port 7.
Described luminaire 1 adopts 6V30W Halogen lamp LED or large power white light LED lamp that light source is provided, and the relative distance of light source and collecting lens is adjustable.
One of core component that described condenser group 2 is patented technology of the present invention, the present embodiment adopts drawplate type structure.Condenser group 2 is between luminaire 1 and objective table 2, and its effect is that the light beam that light source is provided changes the Uniform Illumination that adaptation microcobjective requires into, to realize the object of polarized light modulation.
The condenser group 2 of the present embodiment drawplate type, mainly by the colour filter bar 23 sequentially arranging from top to bottom, the polarizer 24, polarization slit converter 21, polarization slit aperture diaphragm 22, condenser aperture diaphragm 25 and condenser 26 form, polarization slit aperture diaphragm 22 is mounted on polarization slit converter 21, can rotate and move horizontally, on polarization slit converter 21, be also provided with transmission through hole, several polarization slit aperture diaphragms 22 are positioned at the front focal plane of described condenser 26, the territory, transparent zone of polarization slit aperture diaphragm 22 is divided into Liang Ge district, respectively complete transmission area T and polarisation intensification modulation district P, brightness in polarisation intensification modulation district P the rotation of the polarizer 24 in Fig. 3 and is changed, reach intensification modulation object, transmission area T is not subject to the rotation of the polarizer 24 in Fig. 3 and changes completely.Polarization slit converter 21 is interior is respectively 10X, 20X, 40X polarization slit aperture diaphragm, can adapt to the requirement that different multiplying object lens are observed; The setting of transmission through hole, is convenient to traditional bright field and observes.Colour filter bar 23 is for various color filters are installed, to change image substrate.Condenser aperture diaphragm 25 is adapted to traditional bright field viewed in transmittance, and modulation phase contrast need be opened into maximum clear aperture while observing.
Objective table 3, for carrying sample or the culture vessel of observation, objective table has vertical, horizontal locomotive function, the scope that wherein vertically moves is not less than 75mm, transverse shifting scope is not less than 135mm, and other correlation technique parameter meets the technical requirement of objective table in GB/T2985-biological microscope standard.
Eyepiece 4 is binocular vision mirrors, adopts hinge type structure, configuration wide-angle plane cocular, and field of view number is 22, and monolateral diopter can regulate within the scope of-5～+ 5 diopters, and eyepiece interpupillary distance can regulate arbitrarily between 48mm～75mm.Eyepiece stalk angle of inclination is 45 degree.
Rough micro-moving mechanism focus adjusting mechanism 5, can be by rotation coarse motion handwheel or fine motion focusing handwheel, make the relative article carrying platform lifting of object lens, to reach the object of focusing, wherein the fine motion scale value of fine motion focusing handwheel is 0.002mm, is provided with coarse motion degree of tightness and regulates and stop means in focus adjusting mechanism, and wherein slack adjuster is for regulating the moment of torsion of coarse motion control hand wheel, prevent that article carrying platform from moving from top to bottom, be called again " gravity flow ".Stop means is when preventing from changing object lens, and high power objective collision article carrying platform and damage object lens also can focus on during the different object lens of convenient operation fast.
Described objective lens 6, is positioned at objective table below, mainly comprises 10X, 20X, 40X modulation phase contrast objective lens and 10X bright field viewing objective, and wherein modulating phase contrast objective lens is one of core component of patent of the present invention, and Fig. 5 is shown in structural representation.Modulation phase contrast objective lens is infinity flat-field achromatic objective lens optical texture, and modulation phase contrast objective lens comprises object lens modulator 61.The back focal plane of each modulation phase contrast objective lens that objective lens 6 comprises is designed to same opposite planar, object lens modulator is set on back focal plane, as shown in Figure 5, object lens modulator 61 is comprised of modulation sheet and metal pedestal, modulation sheet adopts glass material that optics permeability is stronger as matrix, on its surface, be coated with different clear apertures again, the rete of transmissivity, make it be divided into three transparent zones, as shown in Figure 6, be respectively transmission area (B), shading region (D), gray area (G), direct projection by polarization slit aperture diaphragm and diffracted beam are gathered in the G of object lens modulator after by the observation sample that has phase gradient and change, B twoth district, D district is light tight, direct light part in light beam is completely by G district, its brightness is not subject to the rotation of the polarizer 24 in Fig. 3 and changes, diffracted beam is when seeing through B district, be subject to the rotation of the polarizer 24 in Fig. 3 and change, thereby make the profile of sample target and background form obvious phase gradient variation, but all there is not phase place in advance or retardation change in light beam, only that the variation of light intensity is acted on to the phase gradient variation of observing object, thereby the contrast that shows as image changes.
During use, the light harvesting light beam of luminaire 1 is by after the polarizer 24, form linearly polarized photon, again by being arranged on the P district of polarization slit aperture diaphragm of the front focal plane of condenser 26, because the P district at polarization slit aperture diaphragm is provided with polarizer (being equivalent to analyzer), make polarized light relative to polarization angle with the rotating and change of the polarizer, to realize the variation of the P district transmitted light beam brightness of controlling polarization slit aperture diaphragm, i.e. modulate light intensity.
Described photography and vedio recording output port 7 is the optics output terminals while gathering for MIcrosope image, coordinates special-purpose photography bayonet socket or adapter, can connect CCD video camera, digital camera or digital single-lens reflex camera, general camera.Photography and vedio recording system adopts 100% logical light, can install set switching pull bar by this and carry out visualization and shooting operation.
The present embodiment can also comprise electrical appliance part, and electrical appliance part mainly comprises brightness regulation knob, power control switch, and supply socket and power supply are controlled and protective device original paper.Product input voltage of the present invention is divided three classes: 230V50/60Hz, and 110V/50/60Hz, the wide voltage source of 85～265V, the power of output is that 6V30W(Halogen lamp LED is special-purpose) or the special use of 3.5V5W(LED luminaire).
Embodiment 2
Rotating disc type polarized light modulation phasecontrast microscope
As shown in Fig. 7～9, the rotating disc type polarized light of the present embodiment modulation phasecontrast microscope, comprises luminaire 1, condenser group 2, objective table 3, eyepiece group 4, rough micro-moving mechanism focus adjusting mechanism 5, objective lens 6, photography and vedio recording output port 7.Condenser group 2 adopts rotary-disk type structure, and the condenser 26, condenser aperture diaphragm 25, polarization slit converter 21, polarization slit aperture diaphragm 22, the polarizer 24 that mainly by sequence from low to uper part, are arranged form.Polarization slit aperture diaphragm 22 is arranged on polarization slit converter 21, can rotate and move horizontally.The present embodiment is compared with the condenser group of embodiment 1, and the key distinction is that polarization slit aperture diaphragm conversion regime adopts rotation to switch, and is also provided with 10X, 20X, 40X polarization slit aperture diaphragm and transmission through hole in polarization slit converter.
The remainder of the present embodiment is identical with embodiment 1.
A kind of polarized light modulation phasecontrast microscope of the present invention's design is applicable to existing transmission and is just putting biology, fluorescence, reverse biologic, fluorescence microscopy observational technique.The selection of drawplate type and two kinds of tactic patterns of rotating disc type, in order to adapt to the requirement of different user.
Finally it should be noted that: these are only the preferred embodiments of the present invention; be not limited to the present invention; for a person skilled in the art; all any modifications of doing within the spirit and principles in the present invention, be equal to replacement, improvement etc., within protection scope of the present invention all should be included in.
Claims (10)
1. a polarized light modulation phasecontrast microscope, comprises luminaire, objective table and eyepiece, and its feature is in also comprising:
Objective lens, it is positioned at objective table below, comprises that several have the modulation phase contrast objective lens of consistance back focal plane, and modulation phase contrast objective lens comprises object lens modulator, and described object lens modulator is arranged on the consistance back focal plane of described each modulation phase contrast objective lens;
Condenser group, it is between luminaire and objective table, comprise order arranges the polarizer, polarization slit converter, condenser aperture diaphragm and condenser from top to bottom, described polarization slit converter is provided with several polarization slit aperture diaphragms corresponding with each modulation phase contrast objective lens.
2. polarized light modulation phasecontrast microscope according to claim 1, is characterized in that, described polarization slit converter is drawplate type converter or rotating disc type converter, is also provided with the transmission through hole of observing for bright field on described polarization slit converter.
3. polarized light modulation phasecontrast microscope according to claim 1, is characterized in that, described condenser group also comprises the colour filter bar that is arranged at polarizer top.
4. polarized light modulation phasecontrast microscope according to claim 1, is characterized in that, described objective lens also comprises bright field viewing objective.
5. polarized light according to claim 1 is modulated phasecontrast microscope, it is characterized in that, several modulation phase contrast objective lens are respectively that 10X, 20X, 40X modulate phase contrast objective lens, and on polarization slit converter, several polarization slit aperture diaphragms are respectively 10X, 20X, 40X polarization slit aperture diaphragm.
6. polarized light according to claim 1 is modulated phasecontrast microscope, it is characterized in that, described several polarization slit aperture diaphragms are positioned at the front focal plane of described condenser, in polarization slit aperture diaphragm, are divided into Liang Ge district, are respectively complete transmission area and polarisation intensification modulation district.
7. polarized light modulation phasecontrast microscope according to claim 1, is characterized in that, described eyepiece is binocular vision mirror, is hinge type structure.
8. polarized light modulation phasecontrast microscope according to claim 1, is characterized in that, also comprises for regulating objective lens with respect to the rough micro-moving mechanism focus adjusting mechanism of lifting of object loading table.
9. polarized light modulation phasecontrast microscope according to claim 8, is characterized in that, described rough micro-moving mechanism focus adjusting mechanism comprises coarse motion slack adjuster and stop means.
10. polarized light modulation phasecontrast microscope according to claim 1, is characterized in that, also comprises the photography and vedio recording output port of optics output while gathering for MIcrosope image.
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| CN104977706A (en) * | 2014-04-10 | 2015-10-14 | 中国科学院化学研究所 | Micro-area accurate region selection imaging/spectrum collection method, device and system |
| CN110388882A (en) * | 2018-04-20 | 2019-10-29 | 骆远 | The quantization differential phase contrast microscopic system of the tropisms transfer functions such as tool |
| CN110709749A (en) * | 2017-06-09 | 2020-01-17 | 电子慕泽雷帕里公司 | Combined bright field and phase contrast microscope system and image processing apparatus equipped therewith |
| CN111505817A (en) * | 2020-04-30 | 2020-08-07 | 河北大学 | Phase contrast microscope system based on polarization encoding and imaging method thereof |
| CN112630950A (en) * | 2020-12-29 | 2021-04-09 | 中国科学院苏州生物医学工程技术研究所 | Embryo monitoring device |
| WO2023130636A1 (en) * | 2022-01-10 | 2023-07-13 | 暨南大学 | Uniaxial crystal-based phase contrast microscopy module, device and method |
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Application publication date: 20140122 |