JP2010273632A5

JP2010273632A5 -

Info

Publication number: JP2010273632A5
Application number: JP2009130881A
Authority: JP
Filing date: 2009-05-29
Publication date: 2012-07-05
Anticipated expiration: 2029-05-29

Claims

A chamber containing a culture vessel containing a culture medium and a biological sample is stored in a state of being shielded from the outside air, and includes a chamber having a transparent portion that transmits light for observation by microscopic observation;
A culture gas supply unit for supplying a culture gas used for culturing the biological sample into the chamber;
A pH detection unit for detecting the pH value of the medium in the culture vessel;
A culture apparatus for microscope observation, comprising a culture gas adjusting unit that adjusts the concentration of the culture gas in the culture gas supply unit so that the pH value of the medium detected by the pH detection unit is in a substantially constant state ;
A stage on which the microscope observation culture apparatus is placed;
An illumination light source for illuminating the biological sample in the culture vessel through the transparent part of the chamber;
An objective lens that collects return light from the biological sample irradiated with the illumination light;
An aiming mechanism for adjusting the focal position of the objective lens by relatively moving the stage and the objective lens in the optical axis direction;
A microscope observation culture system comprising: an observation unit that observes the return light condensed by the objective lens.

The pH detection unit detects fluorescence generated from the pH sensitive substance by irradiating the culture medium to which the pH sensitive substance has been added through the transparent part of the chamber, and acquires fluorescence information. The microscope observation culture system according to claim 1, further comprising: a fluorescence information acquisition unit; and a calculation unit that calculates a pH value of the medium based on the fluorescence information acquired by the fluorescence information acquisition unit.

The observation unit includes an image information acquisition unit that images the return light collected by the objective lens and acquires image information;
As the fluorescence information acquisition unit, the image information acquisition unit detects the fluorescence generated from the pH sensitive substance of the medium and collected by the objective lens, and acquires a fluorescence image of the pH sensitive substance. Function,
The microscope observation culture system according to claim 2, wherein the calculation unit calculates a pH value of the culture medium based on the fluorescence image of the pH sensitive substance acquired by the image information acquisition unit .

The observation unit includes an image information acquisition unit that images the return light collected by the objective lens and acquires image information;
The fluorescence information acquisition unit detects fluorescence generated from the pH-sensitive substance of the culture medium in the direction of transmission of the illumination light to acquire the fluorescence information, and the fluorescence information acquired by the fluorescence information acquisition unit The microscope observation culture system according to claim 2, wherein the calculation unit calculates a pH value of the medium based on the calculation unit .

The microscope observation culture system according to claim 1, wherein the observation unit includes an image information acquisition unit that captures the return light collected by the objective lens and acquires image information .

A storage unit for storing the image information acquired by the image information acquisition unit;
The image information acquisition unit acquires the image information at a predetermined time interval and stores the image information in the storage unit. The interval between the time when the image information acquisition unit does not acquire the image information, the pH detection unit The microscope observation culture system according to claim 3, wherein the culture gas adjustment unit controls the concentration of the culture gas while detecting a pH value .

The microscope observation culture system according to claim 2, wherein the pH detection unit includes a light source for pH detection that generates the excitation light irradiated on the culture medium .

The microscope observation culture system according to claim 7, comprising a plurality of the fluorescence information acquisition units, and an optical path selection unit that selectively divides the wavelengths of the fluorescence incident on the plurality of fluorescence information acquisition units .

A chamber containing a culture vessel containing a culture medium and a biological sample is stored in a state of being shielded from the outside air, and includes a chamber having a transparent portion that transmits light for observation by microscopic observation;
A culture gas supply unit for supplying a culture gas used for culturing the biological sample into the chamber;
A pH detection unit for detecting the pH value of the medium in the culture vessel;
A culture apparatus for microscope observation, comprising: a culture gas adjustment unit that adjusts the concentration of the culture gas in the culture gas supply unit so that the pH value of the culture medium detected by the pH detection unit is in a substantially constant state.

The pH detection unit is configured to irradiate the culture medium, to which the pH sensitive substance has been applied, with excitation light via the transparent part of the chamber, and the pH sensitive substance of the culture medium that has been irradiated with the excitation light. A fluorescence information acquisition unit that detects fluorescence generated by excitation of the fluorescence and acquires fluorescence information; and a calculation unit that calculates a pH value of the medium based on the fluorescence information acquired by the fluorescence information acquisition unit. The culture device for microscope observation according to claim 9 provided.

The culture apparatus for microscope observation according to claim 10, further comprising: an optical path selection unit that includes a plurality of the fluorescence information acquisition units and selectively divides wavelengths of the fluorescence incident on the plurality of fluorescence information acquisition units.