WO2007118209A3 - Apparatus and method for rapid detection of analytes - Google Patents

Apparatus and method for rapid detection of analytes Download PDF

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Publication number
WO2007118209A3
WO2007118209A3 PCT/US2007/066172 US2007066172W WO2007118209A3 WO 2007118209 A3 WO2007118209 A3 WO 2007118209A3 US 2007066172 W US2007066172 W US 2007066172W WO 2007118209 A3 WO2007118209 A3 WO 2007118209A3
Authority
WO
Grant status
Application
Patent type
Prior art keywords
sample
system
light beam
excitation light
rapid detection
Prior art date
Application number
PCT/US2007/066172
Other languages
French (fr)
Other versions
WO2007118209A2 (en )
Inventor
Ikro Joe
Myung L Kim
Hoshin Park
Ryan Morlok
Alan Shinn
Chiko Fan
Elias Elias
Original Assignee
Kim Lab
Ikro Joe
Myung L Kim
Hoshin Park
Ryan Morlok
Alan Shinn
Chiko Fan
Elias Elias
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by the preceding groups
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/645Specially adapted constructive features of fluorimeters
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by the preceding groups
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00Features of devices classified in G01N21/00
    • G01N2201/02Mechanical
    • G01N2201/022Casings
    • G01N2201/0221Portable; cableless; compact; hand-held

Abstract

The system of the present disclosure is a system for real time, rapid detection of a targetanalyte in a liquid sample, the system comprising: a sample holder (30) to hold the liquid sample wherein the sample holder is adapted and disposed for rotational motion; a optical source (22) to provide an excitation light beam to excite a fluorescence marker in the sample to emit an emission light; an objective lens (35) to focus the excitation light beam in the sample wherein at least a portion of the optical source is adapted and disposed for translational motion whereby the excitation light beam translates along at least a portion of the sample holder; a detector (70) to record the intensity of the emission light over the period of time; and a member (74) to calculate the number of cells or microorganisms in the sample from the recorded intensity of the emission light over the period of time.
PCT/US2007/066172 2006-04-07 2007-04-06 Apparatus and method for rapid detection of analytes WO2007118209A3 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US79020606 true 2006-04-07 2006-04-07
US60/790,206 2006-04-07

Publications (2)

Publication Number Publication Date
WO2007118209A2 true WO2007118209A2 (en) 2007-10-18
WO2007118209A3 true true WO2007118209A3 (en) 2008-09-04

Family

ID=38581847

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/066172 WO2007118209A3 (en) 2006-04-07 2007-04-06 Apparatus and method for rapid detection of analytes

Country Status (1)

Country Link
WO (1) WO2007118209A3 (en)

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GB2457481B (en) * 2008-02-14 2012-08-08 Starna Scient Ltd Fluorescence measurement cell
JP5877155B2 (en) 2010-07-26 2016-03-02 オリンパス株式会社 Method for detecting the lean particles in a solution by using a light-emitting probe
JP5945506B2 (en) 2010-11-25 2016-07-05 オリンパス株式会社 Optical analyzer and an optical analysis method using the wavelength characteristics of light of a single light emitting particles
EP2667183A4 (en) 2011-01-20 2017-05-10 Olympus Corporation Photoanalysis method and photoanalysis device using detection of light from single light-emitting particle
CN103460026B (en) 2011-03-29 2015-06-10 奥林巴斯株式会社 Photometric analysis device, photometric analysis method, and computer program for photometric analysis, using single light-emitting particle detection
CN103477210B (en) 2011-04-13 2015-09-23 奥林巴斯株式会社 Analysis by the light emitting means single particle detection, optical analysis method, and computer program for optical analysis
JP6013328B2 (en) * 2011-04-18 2016-10-25 オリンパス株式会社 Quantification method of target particles
WO2013021687A1 (en) 2011-08-11 2013-02-14 オリンパス株式会社 Method for detecting target particles
CN103733049B (en) 2011-08-15 2016-01-20 奥林巴斯株式会社 Analysis by the light emitting means single particle detection, optical analysis method, and computer program for optical analysis
EP2749868A4 (en) 2011-08-26 2015-05-27 Olympus Corp Single-particle detector using optical analysis, single-particle detection method using same, and computer program for single-particle detection
JP6010034B2 (en) 2011-08-30 2016-10-19 オリンパス株式会社 The method for detecting a target particle
CN103765197B (en) 2011-08-30 2016-03-02 奥林巴斯株式会社 Analysis by the light emitting means single particle detection, optical analysis method, and computer program for optical analysis
WO2013069504A1 (en) 2011-11-10 2013-05-16 オリンパス株式会社 Spectroscopy device, spectroscopy method, and computer program for spectroscopy, employing individual light-emitting particle detection
JP5996625B2 (en) 2012-02-17 2016-09-21 オリンパス株式会社 Optical spectrometer using a single particle detection, photometric methods and optical analysis computer program
EP2818850B1 (en) 2012-02-22 2016-08-03 Olympus Corporation Method for detecting a target particle
EP2829614A4 (en) 2012-03-21 2016-03-16 Olympus Corp Method for detecting target nucleic acid molecule
JP6010114B2 (en) 2012-04-18 2016-10-19 オリンパス株式会社 Single particle detection apparatus using an optical analysis, a single particle detection methods and single particle detection computer program
WO2013157283A1 (en) 2012-04-18 2013-10-24 オリンパス株式会社 Method for detecting target particles
KR20150041667A (en) 2012-08-24 2015-04-16 가부시끼가이샤 사따께 Method for examining microorganism and device for same
WO2015052965A1 (en) 2013-10-07 2015-04-16 オリンパス株式会社 Photometric analysis device employing single light-emitting particle detection, photometric analysis method, and computer program for photometric analysis

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6566508B2 (en) * 2000-07-07 2003-05-20 3M Innovative Properties Company Fluorogenic compounds and uses therefor
US20040072335A1 (en) * 1999-05-17 2004-04-15 Boege Steven J. Optical instrument including excitation source
US20060068409A1 (en) * 2000-11-27 2006-03-30 Phan Brigitte C Use of restriction enzymes and other chemical methods to decrease non-specific binding in dual bead assays and related bio-discs, methods, and system apparatus for detecting medical targets

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040072335A1 (en) * 1999-05-17 2004-04-15 Boege Steven J. Optical instrument including excitation source
US6566508B2 (en) * 2000-07-07 2003-05-20 3M Innovative Properties Company Fluorogenic compounds and uses therefor
US20060068409A1 (en) * 2000-11-27 2006-03-30 Phan Brigitte C Use of restriction enzymes and other chemical methods to decrease non-specific binding in dual bead assays and related bio-discs, methods, and system apparatus for detecting medical targets

Also Published As

Publication number Publication date Type
WO2007118209A2 (en) 2007-10-18 application

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