JP2009118756A - Cultivation medium for mushroom and method for cultivating mushroom - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide cultivation medium for mushrooms with which mushrooms reliably containing pantothenic acid at a high concentration are cultivated by a simple method: and to provide a method for cultivating mushrooms. <P>SOLUTION: The cultivation medium for mushrooms grown via mushroom-bed cultivation is obtained by adding pantothenic acid calcium into medium base material. The pantothenic acid calcium is added at ≤2g per 100 ml of the cultivation medium. The method for cultivating mushrooms comprises adding the pantothenic acid calcium to the medium base material, charging the medium base material mixed with the pantothenic acid calcium in a container, subjecting the mixture to sterilization, inoculating spawns into the mixture to culture the spawns at an appropriate temperature and moisture to generate and grow up a fruit body. Another version of the method for cultivating mushrooms comprises soaking bed logs cut off from an original tree in soaking water comprising pantothenic acid calcium water solution, inoculating spawns into the bed logs subjected to soaking treatment, aging the spawns, and thereafter soaking the bed logs in the soaking water comprising the pantothenic acid water solution to generate and grow up the fruit body. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

  The present invention relates to a culture medium for mushroom cultivation by fungus bed cultivation or log cultivation and a mushroom cultivation method.

Conventionally, there has been a method for increasing the nutritional value of grown mushrooms by adding vitamins and minerals to the cultivation medium for mushrooms. For example, the cultivation medium and cultivation method for shiitake mushrooms containing high concentrations of thiamine (vitamin B1) disclosed in Patent Document 1 are the growth and fruiting bodies of shiitake mycelium in the cultivation medium for shiitake mushroom bed cultivation. Shiitake cultivated shiitake containing a high concentration of thiamine in the fruit body by containing thiamine in an amount necessary for the formation and development of the fruit.
JP 2003-169538 A

  In the case of the above conventional technique, it is possible to cultivate shiitake containing a high concentration of thiamine, but it does not contain other components at a high concentration. For example, pantothenic acid (vitamin B5), which is widely contained in foods but has a low content, is known to have various functions. For example, maintaining skin health, burning lipids and sugars, increasing stress resistance, increasing good cholesterol, improving immunity, detoxifying harmful chemical compounds, making autonomic neurotransmitters, regulating action, etc. There is a function. When pantothenic acid is deficient, symptoms such as dermatitis, insomnia and malaise appear.

  However, because the content in food is small, if you try to ingest a large amount from a meal for therapeutic purposes or nutritional improvement, you must eat a large amount of food that contains it, and efficient large intake It was a difficult nutrient. For this reason, cultivation of highly functional foods containing a large amount of pantothenic acid is required.

  On the other hand, mushrooms contain a relatively large amount of pantothenic acid and absorb pantothenic acid better than other crops. Therefore, focusing on this point, we researched whether a food containing pantothenic acid at a high concentration could be made.

  The present invention has been made in view of the above problems of the background art, and provides a mushroom cultivation medium and a mushroom cultivation method for cultivating mushrooms that reliably contain pantothenic acid at a high concentration by a simple method. For the purpose.

  The present invention is a mushroom cultivation medium in which calcium pantothenate is mixed with a medium base material in a mushroom cultivation medium by fungus bed cultivation. The said calcium pantothenate is mix | blended in the ratio of 2 g or less per 100 ml of said culture media for cultivation.

  In addition, calcium pantothenate is mixed with the medium base material, the base material mixed with the calcium pantothenate is packed in a container, sterilized, inoculated with inoculum and cultured at a suitable temperature and humidity to generate fruiting bodies. It is a cultivation method of mushrooms to grow. The calcium pantothenate is blended at a rate of 2 g or less per 100 ml of the cultivation medium.

  The present invention also includes a cultivation medium packed in a container, sterilized, inoculated with an inoculum, cultured at an appropriate temperature and humidity, subjected to a bacteria scraping treatment, and a bacteria scraping water made of calcium pantothenate aqueous solution. In addition, a mushroom cultivation method for generating and growing fruit bodies. The concentration of the calcium pantothenate aqueous solution of the fungus scraping water is dissolved in a solvent so that the input amount is 1 g or less of calcium pantothenate per 100 ml of the culture medium, and the solvent is desalted deep ocean water.

  In addition, the present invention inoculates an inoculum on a hoda tree cut from a raw wood, and at least before or after the inoculation with the immersion water made of an aqueous calcium pantothenate solution, the inoculum of the inoculum This is a method for cultivating mushrooms by ripening a hoda tree and then allowing a calcium pantothenate aqueous solution to permeate the hoda tree as needed to generate and grow fruit bodies.

  Moreover, it is the cultivation method of the mushroom which makes the fruit body generate | occur | produce and grow by immersing the holly tree or the culture medium which grew by growing the fruit body with the immersion water made with the calcium pantothenate aqueous solution.

  The solvent for dissolving the calcium pantothenate is desalted deep ocean water.

  Moreover, it is the cultivation method of the mushroom which waters the calcium pantothenate aqueous solution from the mixing | blending of the culture medium for cultivation to harvesting.

  The mushroom cultivation method and mushroom cultivation medium of the present invention can cultivate functional mushrooms containing a high concentration of pantothenic acid by a simple method.

  Hereinafter, embodiments of the present invention will be described with reference to the drawings. FIG. 1 shows a first embodiment of the present invention, which is a method for cultivating enokitake and a culture medium for cultivation. Calcium pantothenate is added to a medium substrate such as sawdust, and the water content is adjusted to an appropriate moisture content to obtain a cultivation medium. The compounding quantity of calcium pantothenate is adjusted so that it may become a ratio of 2 g or less per 100 ml of culture medium. Thereafter, the culture medium is filled in a bottle, and the medium base material together with the bottle is sterilized by heating and cooled. The inoculum of enokitake is inoculated into the sterilized mushroom cultivation medium and cultured at a predetermined temperature for a certain period. Thereafter, the bacteria are scraped and water is poured with the bacteria scraping water made of calcium pantothenate aqueous solution. The water injection method is irrigation or spraying. Calcium pantothenate of bacterial scraping water is dissolved in a solvent so that the input amount is 1 g or less per 100 ml of the culture medium. The solvent is desalted deep ocean water. After that, when restrained, rolled up, and cultured at a predetermined temperature, the fruit body grows, and when grown to an appropriate size, the fruit body is harvested, packaged and shipped.

  When pouring the fungus scraping water, a water pouring device may be used. In the water injection device, the water injection pipes are arranged almost horizontally, and water injection devices having through holes through which the fungus scraping water falls in the middle of the water injection are provided at equal intervals. The water supply pipe is provided with a supply device for supplying fungus scraping water. And the container in which the culture medium was put under the water injector was put in order, and the fungus scraping water was supplied to the water injection pipe by the supply device, and the fungus scraping water was automatically poured into the culture medium.

  According to the mushroom cultivation method and mushroom cultivation medium of this embodiment, enokitake mushrooms containing calcium pantothenate at a high concentration can be obtained by using calcium pantothenate or calcium pantothenate in the mushroom production process, A highly functional food having the function of pantothenic acid can be provided. Pantothenic acid is a nutrient necessary for the growth of mushrooms, and because it is well absorbed by mushrooms, the yield of fruiting bodies is also increased and stabilized.

  The mushroom cultivation method and cultivation medium of this embodiment may be a shimeji cultivation method and cultivation medium. In the case of the shimeji cultivation method, the step of cigarette can be omitted. The solvent for the fungus scraping water may be tap water or the like in addition to the desalted deep ocean water.

  Next, a second embodiment of the present invention will be described with reference to FIG. This embodiment is a cultivation method such as shiitake mushroom or nameko and a cultivation medium. Calcium pantothenate is added to a medium substrate such as sawdust, and the water content is adjusted to an appropriate moisture content to obtain a cultivation medium. The compounding quantity of calcium pantothenate is adjusted so that it may become a ratio of 2 g or less per 100 ml of cultivation medium. Thereafter, the cultivation medium is packed in a bottle or bag, and the medium substrate is sterilized by heating and cooled together with the bottle or bag. A sterilized mushroom cultivation medium is inoculated with inoculum of shiitake mushrooms or sea cucumbers and cultured at a predetermined temperature for a certain period. After that, the fruit body grows when the culture is continued at a predetermined temperature. When the cultivation medium is dried in the growing process, an aqueous calcium pantothenate solution is added. The method of adding water includes spraying, irrigation, addition, etc. in addition to watering. The solvent of the calcium pantothenate aqueous solution to be sprinkled may be desalted deep ocean water. When the fruit body grows to an appropriate size, the fruit body is harvested, packaged and shipped.

  The fungus bed after harvesting is rested and then immersed in immersion water made of calcium pantothenate aqueous solution. The concentration of the aqueous solution of calcium pantothenate in the immersion water is dissolved in a solvent so as to be 5 times or less of 2 g per 100 ml of the culture medium. The solvent of the immersion water may be desalted deep ocean water. The fruit body grows again by the dipping process, and when the fruit body grows to an appropriate size, the fruit body is harvested, packed in a bag, and shipped. When the fruit body is continuously generated without resting the fungus bed, the fruit body is grown by watering the calcium pantothenate aqueous solution without resting after harvesting.

  According to the mushroom cultivation method and mushroom cultivation medium of this embodiment, shiitake mushrooms and sea cucumbers containing calcium pantothenate at high concentrations can be grown.

  Next, a third embodiment of the present invention will be described with reference to FIG. This embodiment is a method for cultivating shiitake mushrooms, sea cucumbers, etc. using a hoda tree. First, a raw wood to be a hoda tree is cut and subjected to an immersion treatment with immersion water made of calcium pantothenate aqueous solution. Then, in order to obtain a suitable moisture content for the hoda tree, a drying process is performed, and chopping into suitable lengths is performed. When purchasing a hoda tree, the purchased hoda tree is dipped in dipping water made of a calcium pantothenate aqueous solution. Inoculate them with inoculum such as shiitake mushrooms and sea cucumbers, tentatively aged as maturation work, face down, and turn over. Next, immersion treatment is performed with immersion water made of calcium pantothenate aqueous solution. The immersion treatment may be performed after inoculation. The concentration of the aqueous solution of calcium pantothenate in the immersion water is dissolved in a solvent so that it is 5 times or less of 2 g per 100 ml of the culture medium. As a result, the child entity is expanded and generated. When the hoda tree dries in the developing step, water is sprayed with a calcium pantothenate aqueous solution to infiltrate the hoda tree. When the fruit body grows to an appropriate size, the fruit body is harvested and shipped.

  After harvesting, the hoda tree is rested and then immersed in immersion water made of calcium pantothenate solution. As a result, the fruit body grows again, and when the fruit body grows to an appropriate size, the fruit body is harvested.

  According to the mushroom cultivation method of this embodiment, shiitake mushrooms and sea cucumbers containing calcium pantothenate at a high concentration can be cultivated using a hoda tree.

  The mushroom cultivation method and mushroom cultivation medium of the present invention are not limited to the above-described embodiment, and can be applied to cultivation methods and cultivation media for agricultural products other than mushrooms. By adding calcium pantothenate to chicken feed in the poultry industry, chicken eggs containing pantothenic acid at a high concentration can be obtained. Even with vitamins and minerals other than calcium pantothenate, high-concentration mushrooms can be cultivated by the mushroom cultivation method and mushroom cultivation medium of the present invention. In addition, calcium pantothenate may not be contained in all of the culture medium, the fungus scraping water, the immersion water, and the watering, and any of them may contain calcium pantothenate. The water may not be deep sea water in which the solvent is desalted in all of the aqueous solution of calcium pantothenate, such as fungus scraping water, immersion water, and water spray, or may be dissolved in tap water or pure water. The type of mushroom to be cultivated may be other than the above.

  Next, a first embodiment of the present invention will be described. This is about the step of immersing with immersion water made of calcium pantothenate aqueous solution after resting the fungal bed harvested by fungus bed cultivation of shiitake mushroom, and the concentration of calcium pantothenate aqueous solution of immersion water, This test examines the relationship between pantothenic acid concentration and calcium concentration in harvested fruit bodies.

  The test method first prepares an aqueous solution (test group 1) that does not contain calcium pantothenate as a control. Then, a 0.25% calcium pantothenate aqueous solution (test group 2) and a 0.50% calcium pantothenate aqueous solution (test group 3) are prepared. Then, the rest of the fungus bed is submerged for 2 days in each of the three test sections. After this, the fruit bodies that have been cultivated at a predetermined temperature and have been developed in sequence are harvested and stored refrigerated. When the yield reaches a certain level, component analysis is performed.

  In addition, as for the date of the test, the component analysis was performed on the fruiting bodies which started flooding from July 19, 2007 (2 days), started growing from July 21, 2007 and harvested by August 5, the same year. .

The results are shown in Table 1 below.

  As a result, it was found that by dissolving calcium pantothenate in the immersion water, pantothenic acid and calcium contained in the fruit body increase.

  Next, a second embodiment of the present invention will be described. This is the fungus scraping water that is poured when the fungus bed of the shimeji mushroom is scraped, with an aqueous solution in which calcium pantothenate is dissolved in the desalted deep ocean water. This is a test to examine the relationship between pantothenic acid concentrations in harvested fruit bodies.

  In the test method, tap water (test zone 1) serving as a control zone is first prepared as fungus scraping water. Then, a 1000-fold diluted solution of demineralized deep sea water in which 0.5% calcium pantothenate was dissolved (test section 2), a 100-fold diluted solution of demineralized deep sea water in which 0.5% calcium pantothenate was dissolved (test) Section 3), demineralized deep sea water stock solution (test section 4) in which 0.5% calcium pantothenate is dissolved is prepared. Then, the inoculum of shimeji mushroom is inoculated into the culture medium, cultured and scraped, and the above-mentioned four test sections are used. Thereafter, the fruit bodies were cultivated at a predetermined temperature, and fruit bodies were harvested.

The results are shown in Table 2 below.

  As a result, it was found that the pantothenic acid contained in the fruit body increases in both the bulk portion and the portion that flips when the calcium pantothenate is dissolved in the bacterial scraping water. Moreover, although the concentration of calcium pantothenate is constant at 0.5% in the test sections 2 to 4, it is understood that the absorption rate of pantothenic acid increases as the concentration of the desalted deep sea water as the solvent increases. It was. As a result, it was found that desalted deep ocean water is effective in improving the absorption rate of calcium pantothenate.

  Next, a third embodiment of the present invention will be described. This is a test to examine the relationship between the concentration of calcium pantothenate in the cultivation medium, the yield of fruiting bodies, and the concentration of pantothenic acid in fruiting bodies, by making a culture medium for enokitake mushroom by mixing the medium base material and calcium pantothenate. It is.

  The test method first prepares a culture medium (test group 1) that does not contain calcium pantothenate as a control group. Then, 300 mg / bin of calcium pantothenate (test group 2), 600 mg / bin of calcium pantothenate (test group 3), and 1500 mg / bin of calcium pantothenate (test group 4) are prepared. And after a sterilization process, a fruit body is grown and harvested through the process of seed | inoculation of an inoculum, culture | cultivation, fungus scraping, and suppression. About the harvested fruiting body, the yield was measured and the component analysis was conducted.

  The test was conducted on July 13, 2007, and harvested on September 1, the same year.

The results are shown in Table 3 below.

  As a result, the yield of fruiting bodies increased in test plots other than test plot 4 by mixing calcium pantothenate with the cultivation medium. Moreover, it turned out that pantothenic acid content becomes higher than the test group 1 in all the test groups.

It is process drawing which shows the cultivation method of the mushroom of 1st embodiment of this invention, and the culture medium for cultivation of a mushroom. It is process drawing which shows the mushroom cultivation method and culture medium for mushroom cultivation of 2nd embodiment of this invention. It is process drawing which shows the cultivation method of the mushroom of 3rd embodiment of this invention.

Claims (8)

  A mushroom cultivation medium, wherein calcium pantothenate is mixed with a medium base material in a mushroom cultivation medium by fungus bed cultivation.   The mushroom cultivation medium according to claim 1, wherein the calcium pantothenate is blended at a rate of 2 g or less per 100 ml of the cultivation medium.   Mix calcium pantothenate with the medium base material, pack the medium base material mixed with the above calcium pantothenate into a container, apply sterilization treatment, inoculate the inoculum here and culture at appropriate temperature and humidity to generate fruiting bodies A method for cultivating mushrooms characterized by growing.   The said pantothenate calcium is mix | blended in the ratio of 2 g or less per 100 ml of said cultivation media, The mushroom cultivation method of Claim 3 characterized by the above-mentioned.   Fill the culture medium in a container, sterilize it, inoculate the inoculum here, incubate at appropriate temperature and humidity, perform fungus scraping treatment, add fungus scraping water made of calcium pantothenate aqueous solution, A method for cultivating mushrooms characterized by generating and growing.   6. The method for cultivating mushrooms according to claim 5, wherein the concentration of the aqueous solution of calcium pantothenate in the scraping water of the fungus is dissolved in a solvent so that the input amount is 1 g or less of calcium pantothenate per 100 ml of the cultivation medium.   Inoculate a hoda tree cut from raw wood with inoculum, and at least before or after that, the hoda tree is dipped in immersion water made of calcium pantothenate solution, and the inoculated inoculum is aged. A method for cultivating mushrooms is characterized in that a calcium pantothenate aqueous solution is further permeated into the above-mentioned hoda tree as needed to generate and grow fruit bodies. The method for cultivating mushrooms according to claim 5, 6 or 7, wherein the calcium pantothenate is dissolved in desalted deep ocean water.

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