JP2009106305A - Clean bench - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a clean bench causing no risk of turning down a petri dish by mistake. <P>SOLUTION: In the clean bench (A) wherein clean air is allowed to flow into a working room (2) forming a work space and experiments and/or observation of bio-specimens are carried out under the sterilized and cleaned environment, a microscope (4) for observation of specimens is detachably attached on an almost center, the work platform under the objective lens is made flattened and an illumination (9) is embedded under the face of the platform. Further, a heater panels (71) are embedded on both right and left sides so as to nip the illumination (9). <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

  The present invention relates to a clean bench configured so that a sample such as a tissue or a cell (for example, a fertilized egg) can be subjected to experiments and observations in a sterile and dust-free environment.

  Conventionally, there has been a clean bench having a general structure as shown in FIG.

  Such a conventional clean bench X forms a work space 200 having a front opening in the machine frame 100, and a sterilization lamp 400 and other illumination lamps (not shown) are provided on the wall surface of the work space 200, and further, a sterilization lamp is used. A gas burner (not shown) or the like is provided, and clean air obtained using a HEPA filter always flows from the ceiling wall 300 into the work space 200 to make the work space 200 an aseptic and dust-free environment. . Reference numeral 310 denotes a work surface.

  In order to observe a biological sample such as a cell, a heater panel 500 for holding the sample at a predetermined temperature and a microscope 600 are separately brought into the work space 200 and placed on the work surface 310. The observer was working with the upper body facing the work space 200 from outside the clean bench.

  Further, the CCD camera 700 may be attached to the microscope 600 so that a person other than the operator of the microscope 600 can view the sample without explaining the contents of the observation / experiment to others or explaining it.

  In such a case, as shown in the figure, the monitor display 800 is installed outside the clean bench X such as on the machine casing 100, for example. Reference numeral 710 denotes a connection cord for connecting the CCD camera 700 and the display 800.

  In addition, as a conventional clean bench, there is one in which a plant is cultured under aseptic conditions by adding a temperature control function to control the temperature of the work space (see, for example, Patent Document 1). .

JP-A-5-256486

  However, as described above, it is troublesome to bring the microscope 600 and the heater panel 500 from the outside. In particular, when the heater panel 500 on which the petri dish 900 or the like containing the sample is placed is placed on the work surface 310, the work surface. If the CCD camera 700 is used, the connection cord 710 may be connected from the work space 200 to the clean bench X. It was getting in the way of being distracted outside.

  Also, in order for the operator to view the monitor display 800, the user must often raise his / her body and put his head out of the work space 200, thus reducing the work efficiency, and the outside of the clean bench X and the work space. There is a risk that the sterilization and dust-free environment in the work space 200 may be damaged as the entrance and exit from the 200 become more frequent.

  An object of the present invention is to provide a clean bench capable of solving the above-described problems.

  Accordingly, in order to solve the above-mentioned problems, in the present invention described in claim 1, clean air is allowed to flow through a working chamber in which a working space is formed, and experiments and observations of biological samples are performed in an aseptic and dust-free environment. On the bench, a microscope for observing the sample was detachably attached to the approximate center of the work table, the work table below the objective lens was a flat surface, and illumination was embedded below the same surface.

  Moreover, in this invention of Claim 2, the heater panel was embed | buried on the left-right side so that the said illumination could be pinched | interposed.

  According to a third aspect of the present invention, an image capturing device is connected to the microscope, and a monitor screen connected to the image capturing device is embedded in the wall surface of the work chamber.

  According to a fourth aspect of the present invention, the monitor screen is embedded on the wall surface in a flush state, and the wall surface in the work chamber is formed flat.

  Further, in the present invention according to claim 5, in a clean bench that conducts experiments and observations of biological samples in a sterile and dust-free environment by flowing clean air into the working chamber in which the working space is formed, a microscope for sample observation is provided. Removably attach to the center of the workbench, cover the lower wall located directly below the microscope with a glass plate on the top, and embed a bench warmer with lighting embedded in the center, and heater panels on the left and right sides. The entire lower wall, which was buried and formed flat on the entire surface, could be heated.

  According to the present invention, a petri dish containing a biological sample or the like is placed in the work chamber and is not accidentally turned over when it is moved.

It is a front view of the clean bench which concerns on 1st Example. It is a side view of the clean bench. It is explanatory drawing of the clean bench. It is a front view of the clean bench which concerns on 2nd Example. It is a side view of the clean bench. It is explanatory drawing of the conventional clean bench.

  The present invention is a clean bench that allows clean air to flow through a working chamber that forms a working space and performs experiments and observations of biological samples in a sterile and dust-free environment. The work table below the objective lens is set as a flat surface, and illumination is embedded below the same surface. In addition, an image capturing device is connected to a microscope, and a monitor screen connected to the image capturing device is embedded in a wall surface in the work chamber.

  In addition, a heater is embedded in the lower wall surface of the work chamber serving as a work table, and the monitor screen and the heater are embedded in the wall surface so that the wall surface in the work chamber is as flat as possible.

  That is, in the work space of the clean bench, a microscope provided with an image capturing device is arranged on a work table, and a monitor screen connected to the image capturing device is embedded in the back wall in a flush state.

  Here, the microscope is detachably attached to the approximate center of the work table, the work table below the objective lens is a flat surface, the illumination is embedded under the same surface, and the heaters are placed on the left and right sides of the illumination so as to sandwich the illumination. Panels are buried.

  Therefore, the entire workbench can be used effectively, and the sample housed in a petri dish or the like can be kept at a predetermined temperature if it is placed on the workbench. Since it can be moved by simply sliding it on the work table without lifting it, there is no risk that it will be accidentally turned over and valuable samples will be wasted.

  In addition, since the wall surface in the work chamber is made as flat as possible, turbulent flow is less likely to occur in the clean air that flows into the work chamber, and even if germs and dust enter the work chamber, It rides smoothly and can flow out smoothly and maintain a sterile and dust-free condition in the working chamber.

  Furthermore, since the operator can see the monitor screen without taking out the upper body from the inside of the sterilized and gradually dusted work space, it is not necessary to look into the microscope, and the degree of fatigue is small. For example, when the sample is explained to a plurality of other people, the explanation can be made while pointing the screen in the work space, so that the work efficiency is remarkably improved.

  Further, a vibration isolation table can be embedded in the lower wall surface of the work chamber serving as a work table. In other words, when performing fine work while looking into the microscope, a high-power microscope is used, but even in this case, slight vibrations that hinder the work can be prevented, so that the high-power microscope can be used without any trouble. .

  Further, an incubator can be provided on the side wall surface of the working chamber. That is, when culturing a biological sample, a work on a clean bench or an operation under a microscope may be required depending on the purpose of the culture. At this time, there is a possibility that the sample and the incubator itself may be contaminated when the sample is taken in and out of the incubator. By using the above configuration, the biological sample can be confirmed, operated, and cultured without contaminating the incubator itself. A series of operations such as these can be performed in a clean bench, and contamination by bacteria can be prevented as much as possible.

  Further, a communication opening with the outside can be provided on the side wall surface of the working chamber. That is, for example, if a biological sample is collected in a clean room provided in the adjacent room, it is possible to enter the clean bench directly from the communication opening without leaving the clean room and passing through the contaminated area, thus preventing contamination of the biological sample. can do.

  Furthermore, it is desirable that the temperature in the work chamber can be controlled from a low temperature to a high temperature. In other words, it is possible to work in an environment most suitable for a biological sample, and at the same time, it is possible to keep the indoor temperature in which the clean bench is installed at a comfortable temperature according to preference.

(First embodiment)
Hereinafter, a first embodiment of the present invention will be described in detail with reference to the drawings.

  1 is a front view of a clean bench A according to a first embodiment, FIG. 2 is a side view thereof, and FIG. 3 is an explanatory view thereof.

As shown in FIGS. 1 to 3, the clean bench A according to the present embodiment is provided with a work chamber 2 having a front opening that forms a work space in a machine frame 1 having a leg portion 11 with a caster 10 attached to a lower end portion. ing.

  Specifically, the work chamber 2 includes a ceiling wall 20 provided in the machine frame 1 and a lower wall 21 serving as a work table, a back wall 22, and left and right side walls 23 and 24. 23 and 24 are made of glass so that they can be well lit.

  In addition, the functional unit storage casing 12 formed in the upper part of the work chamber 2 stores a blower fan and a HEPA filter (not shown). The exhaust port 30 provided on the entire surface of the ceiling wall 20 and the lower wall 21 The intake port 31 provided on the front end side is communicated through a ventilation path formed in the lower wall 21 and the inner wall 22 so that clean air sterilized and gradually dusted can be circulated through the HEPA filter. The space 2 can be maintained in a sterile and dust-free state. In FIGS. 1 and 2, 13 is an operation panel, 14 is an outlet, 15 is a gas hose connection port for a gas burner, and 16 is a gas stopper for the gas burner.

  In the above configuration, the present invention is characterized in that the microscope 4 for sample observation is mounted in the work chamber 2 and a CCD camera 5 is connected to the microscope 4 as an image capturing device and connected to the camera 5. The monitor screen 6 is embedded in the wall surface in the work room 2, that is, the back wall 22.

  That is, the microscope 4 is detachably supported and fixed substantially at the center of the lower wall 21 of the work chamber 2, and the monitor screen 6 electrically connected to the CCD camera 5 is flush with the wall surface at the lower left portion of the back wall 22. Buried in the state. Reference numeral 41 denotes a support stand for the microscope 4.

  Therefore, there is no need to bring the microscope 4 from the outside, and not only the person who operates the microscope 4 but also the person outside the clean bench A can observe the sample. It is also possible to explain while watching the monitor screen 6 simultaneously with a plurality of other persons without taking out the upper body from the inside.

  In addition, the operator does not have to look into the lens barrel of the microscope 4 one by one, and adjustment of the monitor screen 6 and the like can be performed in the work chamber 2, so that work efficiency is improved.

  Further, in the present invention, the heater 7 is embedded in the surface of the lower wall 21 in the work chamber 2 serving as a work table, and the monitor screen 6 and the heater 7 are embedded in a flush state with respect to the wall surface. Another characteristic is that the entire wall surface is formed as flat as possible.

  That is, in the present embodiment, as the heater 7, a bench warmer 70 whose upper surface is covered with the glass plate 8 is embedded in the lower wall 21 located immediately below the microscope 4, and a heater panel 71 is embedded on the left and right sides thereof. The entire lower wall 21 formed flat on the entire surface can be heated. Reference numeral 9 denotes illumination embedded in the center of the bench warmer 70.

  By the way, the temperature of the heater 7 is adjusted by a controller 17 provided on the left side of the front surface of the machine casing 1.

  With this configuration, the petri dish S containing a sample such as a cell can be held at any temperature on the lower wall 21. Moreover, since the work table is flat, the petri dish S does not need to be lifted and moved. It can be moved to a desired position simply by causing it to be accidentally turned over.

  Further, since the entire inner wall surface in the work chamber 2 is made as flat as possible, turbulent flow is less likely to occur in the clean air flowing into the work chamber 2, and the clean air flowing out from the exhaust port 30 flows into the intake port 31. Is sucked smoothly.

  Therefore, even if germs and dust enter the work chamber 2, the air circulates smoothly and passes through the HEPA filter, so that the sterile and dust-free state in the work chamber 2 is maintained well. can do.

(Second embodiment)
Next, a clean bench B according to a second embodiment will be described with reference to FIGS. In addition, about the same component, it has shown with the same code | symbol.

  This has basically the same configuration as the clean bench A described in the first embodiment, but a vibration isolation table 18 is embedded in the surface of the lower wall 21 in the work chamber 2 serving as a work table. In addition, the incubator 19 is provided on one side wall surface of the work chamber 2, here, the left side wall 23 surface, and a pass box serving as a communication opening to the outside on one side wall surface of the work chamber 2, here, the rear wall surface 22. 25, and the inside of the work chamber 2 can be controlled from a low temperature to a high temperature.

  The vibration isolation table 18 may be an existing product of an air spring type, but this is embedded in the lower wall 21 of the work chamber 2 in a flush state, and a high magnification microscope 40 is installed thereon. is doing. That is, there is no problem that the position of the high-power microscope 40 placed thereon is higher than when the separated vibration isolation table is disposed in the work chamber 2, and the work space is narrowed by the vibration isolation table. I don't have to.

  In addition, since the incubator 19 is provided, a series of operations such as confirmation, operation, and culture of biological samples can be performed in the clean bench B without contaminating the incubator 19 itself, and contamination by bacteria or the like is possible. Can be prevented. 19a is a sliding door.

  Further, as shown in FIG. 5, the pass box 25 communicates with a clean room R provided in an adjacent room, and upper and lower slide doors 25a are attached to both openings.

  If a biological sample or the like is collected in the clean room R by the pass box 25, it can be put directly into the clean bench without going out of the clean room R and passing through the contaminated area, thereby preventing contamination of the biological sample. it can.

  Furthermore, the temperature inside the work chamber 2 can be controlled from low temperature to high temperature, so that work can be performed in an environment most suitable for biological samples. At the same time, the temperature in the room where the clean bench B is installed is Apart from B, it becomes possible to keep the comfortable temperature according to preference, and the work environment is improved.

  As mentioned above, although this invention was demonstrated through the Example, this invention is not limited to each above-mentioned Example.

  For example, the size and structure of the machine casing 1 can be changed as appropriate, and the specification can be changed as appropriate, such as disposing a germicidal lamp or the like in the work chamber 2 or providing an appropriate storage table.

A Clean bench 1 Machine frame 2 Work room 4 Microscope 5 CCD camera (image capture device)
6 Monitor screen 7 Heater 9 Illumination
21 Lower wall
22 back wall

Claims (5)

In a clean bench that conducts clean air in a sterile, dust-free environment by flowing clean air through the work chamber that forms the work space.
A clean bench characterized in that a microscope for observing a sample is detachably attached to the approximate center of a workbench, the workbench below the objective lens is a flat surface, and illumination is embedded under the same surface.   The clean bench according to claim 1, wherein heater panels are embedded on the left and right sides so as to sandwich the illumination.   The clean bench according to claim 1 or 2, wherein an image capturing device is connected to the microscope, and a monitor screen connected to the image capturing device is embedded in a wall surface in a work chamber.   The clean bench according to any one of claims 1 to 3, wherein the monitor screen is embedded in a wall surface in a flush state, and the wall surface in the work chamber is formed flat. In a clean bench that conducts clean air in a sterile, dust-free environment by flowing clean air through the work chamber that forms the work space.
A microscope for observing the sample is detachably attached to the approximate center of the workbench. A clean bench that features a heater panel embedded on the left-hand side, allowing the entire lower wall to be heated flat.

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