JP2009023984A - Lipolysis promoter and food and beverage - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To obtain a new lipolysis promoter and foods and beverages each promoting lipolysis of accumulated fat in fat cells, carrying out suppression, prevention and improvement of obesity, having high safety and derived from nature. <P>SOLUTION: The lipolysis promoter and the food and the beverage each comprises both or either one of Aronia melanocarpa or Aronia melanocarpa processed residue or both or either one of water- and organic solvent-extracts thereof as active ingredient(s). <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

  The present invention is a lipolysis promoter comprising as an active ingredient an aronia and an aronia processed residue that can be used for pharmaceuticals, foods, health foods, foods for specified health, etc., or their water or organic solvent extracts, and The present invention relates to a food or drink containing a lipolysis promoter.

  Fat is stored in white adipose tissue as surplus energy taken as food. Excess white adipose tissue accumulates obesity, resulting in various lifestyle-related diseases as well as a major cosmetic problem. Scientific evidence reveals that metabolic syndrome syndrome such as hypertension, insulin resistance, impaired glucose tolerance, and hyperlipidemia may be caused by the increase in white adipose tissue that has recently formed around the viscera (mesenteric fat) Therefore, prevention and improvement are socially eagerly desired.

  For the prevention and improvement of obesity, various ingredients have been studied, such as dietary fiber that delays and inhibits dietary restriction and excess energy absorption, and substances that inhibit carbohydrate absorption in the digestive tract. However, restriction of energy intake also causes a decrease in basal metabolic rate, and obesity may not always be improved. Therefore, in order to eliminate obesity, it is ideal that the accumulated fat is actively metabolized and decomposed and released as heat energy. For these reasons, in recent years, functional ingredients having a lipolysis promoting action have been actively searched from food materials, and many lipolysis accelerators and foods and drinks have been proposed.

  Up to now, as a naturally occurring lipolysis accelerator, a lipolysis accelerator characterized by containing a citrus plant as an active ingredient (see Patent Document 1), comprising a plant of thistle family as an active ingredient The same agent (see Patent Document 2), the same agent containing a pepper family plant as an active ingredient (see Patent Document 3), orange leaf, orange flower, dandelion leaf and kalamas root, or an extract thereof As an active ingredient (see Patent Document 4), an extract extracted from at least one material selected from the group consisting of pearl barley, barley, Seiko, Goseki coffee and Pu'er tea The same agent (see Patent Document 5), and more recently, the same agent (see Patent Document 6) containing lotus or its extract as an active ingredient, birch family birch extract. The same agent containing at least one of a liquid and an extract of Gramineae (see Patent Document 7), a fat accumulation inhibitor and promoter containing a hydrolyzate of wheat protein (see Patent Document 8), and the like. A number of lipolysis promoters and foods and drinks are disclosed.

On the other hand, aronia in the present invention is a plant belonging to the family Rosaceae native to North America ( Aronia melanocarpa ), and fruit juice may contain abundant vitamin C, carotenoids, dietary fiber and the like in addition to anthocyanins, which are a kind of polyphenols. Are known. It is a northern fruit with such a healthy component and has various physiological functions such as stress, arteriosclerosis, stool deodorization, antioxidant effect, gastric ulcer, immune enhancement and constipation improvement so far In addition to those known, angiotensin inhibitors (see Patent Document 9), cyclooxygenases, inhibitors of inducible nitric oxide synthase, foods (see Patent Document 10), and the like are disclosed. However, it is not known that aronia and aronia processing residues in the present invention, or extracts thereof, have an action of promoting lipolysis.
JP-A-8-81382 JP-A-8-301780 JP-A-8-245410 JP-A-11-228431 JP 2002-275078 A JP 2004-307365 A JP 2006-045120 A JP 2007-106683 A JP 2001-172191 A JP-A-2005-213242

  The present invention includes a naturally occurring novel lipolysis accelerator and a lipolysis accelerator that are capable of promoting the degradation of fat accumulated in fat cells and suppressing, preventing and improving obesity and having high safety. It aims at providing the food and drink which are made.

  As a result of intensive studies to solve the above-mentioned problems, the present inventors have found that aronia and aronia processing residues or extracts thereof have lipolysis promoting activity in adipocytes, and complete the present invention. It came to.

  That is, this invention relates to the lipolysis promoter which uses extracts, such as aronia and an aronia processing residue, as an active ingredient. Furthermore, this invention relates to the food / beverage products containing a lipolysis promoter, such as aronia and an aronia processing residue.

  The aronia of the present invention is an edible fruit tree, and fruit and fruit juice, processed residue after fruit juice pressing, or all of them may be used.

  The aronia and the aronia processing residue correspond to those obtained by drying and pulverizing, or those extracted by using a solvent such as water or an organic solvent.

The lipolysis promoter of the present invention can be used as an obesity preventing agent or an obesity improving agent. Moreover, the food-drinks in this invention are characterized by containing the said lipolysis promoter.

By using the allonia and / or aronia processing residue of the present invention, or these water and organic solvent extracts, the active ingredients contained in these promote the decomposition of fat in fat cells, and obesity It is possible to provide a lipolysis promoter that can be prevented and improved, and a food and drink comprising the same.

Hereinafter, preferred embodiments of the present invention will be specifically described.
The organic solvent used for the extraction of aronia and aronia processing residues of the present invention includes water, alcohols such as methanol and ethanol, ethyl acetate, acetone, etc., among which strong lipolysis promoting action is obtained. A 10 to 99% by weight aqueous alcohol solution is preferred. As the alcohol, methanol and ethanol are preferable. As the extraction method, any method such as extraction by immersion, heating extraction, continuous extraction, supercritical extraction, or the like may be used. In order to obtain an extract using the above-mentioned solvent, it is sufficient to follow a known method. For example, after using aronia and aronia processing residue as a raw material and appropriately crushing this, the pulverized product is known with the above-mentioned solvent. The method is used for extraction. Specifically, the solvent is 1 to 100 times (weight ratio), preferably 3 to 30 times (weight ratio) of the raw material, left at room temperature for 1 to 7 days, or heated to reflux at 40 to 60 ° C. for 2 to 16 hours. While extracting.

  The extract obtained as described above may be used in the present invention as it is, but preferably the water and organic solvent used in the extraction are distilled off by an appropriate method such as concentration under reduced pressure, evaporation, or lyophilization. Is good. Furthermore, in order to obtain a stronger lipolysis promoting action, the extract is obtained by concentrating the active ingredient by solvent fractionation using a solvent such as water, methanol, ethanol, propanol, chloroform, ethyl acetate, acetone or the like. be able to.

  The lipolysis promoter of the present invention can be used in any form such as an oral preparation and an external preparation. That is, it may be formulated into a form that can be easily used as a drug, such as tablets, capsules, granules, etc., using excipients as appropriate. Furthermore, the lipolysis promoter can be used to reduce fat locally by applying it directly to the face and abdomen for cosmetic purposes, such as lotion, skin lotion, ointment, poultice, sheet. It can be used as an agent.

  The blending amount of the lipolysis accelerator of the present invention varies depending on each application, but can be used in a wide range. For example, in the case of an oral preparation, it is 0.01 to 10 g per day for an adult, preferably 0.1 to 5 g, and can be appropriately increased or decreased. Moreover, in the case of an external preparation, it is preferable to mix | blend 0.05 to 50% in a composition.

  In addition, foods and drinks containing the lipolysis promoter of the present invention are not particularly limited to foods that can be blended in addition to health foods and foods for specified health use that are intended to promote health through obesity-preventing action or obesity-improving action. Specifically, in addition to health supplements in the form of tablets, powders, capsules, tablet confectionery, chewable tablets, foods for specified health use and nutritional supplements, beverages such as soft drinks, tea drinks, sports drinks, chocolate, Confectionery such as candy can be mentioned, and the blending amount thereof is preferably 0.0001% or more, particularly 0.01 to 99% by weight in the food or drink in terms of the solvent-extracted dried product.

  Hereinafter, the present invention will be specifically described with reference to examples. However, the present invention is not limited to the following examples.

Example 1
[Preparation of Aronia extract]
Aronia was obtained by freeze-drying the residue after pressing the fruit juice (50 g), adding 20 times amount (weight ratio) of 99% ethanol extract, and extracting in the dark at room temperature for 7 days. A 20-fold amount (weight ratio) of the extract was newly added to the residue excluding the extract (primary extract) in the same manner as described above, and extracted under the same conditions to obtain a secondary extract. The primary extract and the secondary extract were combined and concentrated under reduced pressure to dryness. The yield of the aronia extract under these conditions was 9.7 g, and the yield was 19.5%.

(Example 2)
Each powder component was uniformly mixed according to the composition shown in Table 1 below, and the mixed powder was compressed to prepare tablets.

(Example 3)
A soft drink was prepared according to the formulation shown in Table 2 below.

(Test Example 1)
[Measurement of lipolytic activity using mouse preadipocytes 3T3L1]
Differentiation of mouse preadipocytes 3T3L1 into adipocytes and measurement of lipolytic activity using differentiated adipocytes were performed as follows. Specifically, mouse preadipocytes 3T3L1 were dispersed in DMEM medium containing 10% calf serum, and 2 mL of 30,000 cells were placed in each well of a 24-well microplate and cultured at 37 ° C. for 48 hours under 5% carbon dioxide gas. did. After 48 hours, the medium was removed by aspiration, and 2 mL of a solution containing 0.5 mM isobutyl xanthine and 1 μM dexamethasone was added to DMEM medium containing 10% fetal bovine serum and cultured again for 48 hours as described above. . After 48 hours, the medium was removed by suction, and 2 mL of medium supplemented with 10 μg / mL insulin was added to DMEM medium containing 10% fetal bovine serum, and cultured as described above. After 48 hours, the medium was removed by aspiration, 2 mL of DMEM medium containing 10% fetal calf serum was added, and the culture was performed in the same manner as described above, and the medium was changed every 48 to 72 hours. Lipolytic activity was measured using differentiated adipocytes that had been cultured for 7 days and in which fat granules were sufficiently accumulated.

  The lipolytic activity was measured by removing the culture medium by suction and then washing the sample twice with PBS, and adding 0.25 mL of the measurement sample solution prepared to a concentration of 100 μg / mL to the Aronia extract at 37 ° C. under 5% carbon dioxide gas. After culturing for 1 hour, 25 μL of the culture supernatant was collected, and glycerol released in the culture supernatant was quantified by an enzymatic method. In addition, it measured similarly using the isopleterenol solution which has a lipolysis acceleration | stimulation effect | action with a beta3 adrenergic agent as a comparative product.

  Using the above method, the lipolytic activity of the aronia extract obtained in the present invention was measured at a concentration of 100 μg / mL. As a result, the lipolytic activity of 10 μM isopreterenol was obtained in the present invention. The aronia extract showed high activity (FIG. 1). Furthermore, this extract showed a concentration-dependent lipolysis promoting action in a concentration range of 25 to 100 μg / mL (FIG. 2).

It is a graph which shows the measurement result of the glycerol density | concentration liberated from the differentiation fat cell (3T3L1). It is a graph which shows the measurement result of the free glycerol density | concentration when changing the addition density | concentration of an aronia extract.

Explanation of symbols

Control: When no aronia extract is added Positive control: When 10 μM isopreterenol is added

Claims (3)

A lipolysis promoter comprising aronia and / or aronia processing residue or an extract thereof as an active ingredient. The lipolysis promoter according to claim 1, wherein the extract is an extract of water and / or an organic solvent. Food / beverage products containing the lipolysis promoter of Claim 1 or 2.

Images