JP2009000065A - Method for producing salt-free or low-salt soybean paste-based seasoning, and product of the seasoning - Google Patents

Method for producing salt-free or low-salt soybean paste-based seasoning, and product of the seasoning Download PDF

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JP2009000065A
JP2009000065A JP2007165017A JP2007165017A JP2009000065A JP 2009000065 A JP2009000065 A JP 2009000065A JP 2007165017 A JP2007165017 A JP 2007165017A JP 2007165017 A JP2007165017 A JP 2007165017A JP 2009000065 A JP2009000065 A JP 2009000065A
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salt
seasoning
miso
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JP4885802B2 (en
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Yoshihiro Hamazaki
吉弘 濱崎
Yoshitaka Honda
芳孝 本多
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MARUSAN I KK
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Abstract

<P>PROBLEM TO BE SOLVED: To provide a western-style salt-free or low-salt soybean paste-based seasoning that prevents rancidity, produced in conventional production of salt-free or low-salt soybean paste and that is caused by various bacteria, such as lactic acid bacteria which have delicious taste of soybean paste without the smell of fermentation, and refreshing sour taste, and which is expanded in utilization field. <P>SOLUTION: A method for producing the salt-free or low-salt soybean paste-based seasoning is constituted of adding at least one kind edible organic acid to a mixture constituted of steamed soybeans, soybean paste malt, 0-5% common salt, and material water followed by mixing, preparing the mixture in a container, and ripening the product in a range of 45-65°C. As a result of this, a seasoning which will not cause production loss, and has preservability and fresh sour taste can be produced. The seasoning is suitable, due to its characteristics, for health maintenance naturally for people, in general, and kidney disease patients, and is advantageous and applicable to cooking field of wide and various kinds of foods. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

本発明は、無塩又は低塩のみそ系調味料の製造法及びその製品に関する。   The present invention relates to a method for producing a salt-free or low-salt miso seasoning and a product thereof.

従来の食塩5〜13%を含有する一般の甘口乃至辛口みその製造法は、蒸煮大豆と麹食塩と種水とから成る配合物を容器に仕込み、乳酸菌、酵母の生育温度に適した30℃前後で発酵作用と共に麹菌の酵素作用により1ヶ月程度以上の長期間かけて熟成させて行い製造する方法と、50℃前後の高温で主として麹菌の酵素作用により5日〜10日間程度の短期間熟成させて製造する方法が行われているが、いずれの場合も、食塩を5〜13%含有するため、その用途は、みそ汁、味噌漬けなど主として日本食の調理に限定される。
その上、上記の比較的低温の30℃前後で発酵、熟成を行う製造法では、乳酸菌や酵母が異常に繁殖し、異常発酵を起こし、品質の低下をもたらし、或いは保存中に湧きを生ずるなどの不都合を生ずる。また、全国味噌技術会2006年3月15日発行の「新・味噌技術ハンドブック」のP70,P71及びP116に記載されているように、食塩含有量が多い場合は問題ないが、食塩含有量が5〜7%と比較的少ないみその製造では、50℃前後の高温で、乳酸菌や酵母の生育を抑止し、主として麹の有する上記酵素の分解作用を主として熟成させる場合でも、耐熱性乳酸菌やBacillus系などの耐熱性細菌の異常増殖による酸敗、腐敗が起こることが報告されている。
一方、近年は、みその用途拡大や一般人の健康保持のため、或いは食塩の摂取が禁じられたり、制限される高血圧症、腎臓病などの患者のため、無塩又は低塩みその開発がなされ、公知である。例えば、下記の特許文献1には、腎臓疾患などの患者に適し、且つ様々な食品に利用し得る無塩又は低塩みそ及びこれらの抽出物を主成分とする機能性組成物に関する発明が開示されている。
また、下記の特許文献2には、穀類を水中に1時間浸漬する工程と、該穀類を水中で穀類が柔らかくなるまで煮沸する工程と、該穀類を潰す工程と、潰した穀類に麹菌を添加し、室内又は冷蔵して熟成させる工程とから成る無塩みその製造方法が開示されている。
全国味噌技術会2006年3月15日発行の「新・味噌技術ハンドブック」のP70,P71及びP116 特開2006-70015公報 特開2004-215626公報
The conventional method for producing sweet or dry mash containing 5 to 13% salt is to prepare a mixture of steamed soybeans, salted salmon and seed water in a container, 30 ° C suitable for the growth temperature of lactic acid bacteria and yeast. A method of aging by fermentation for about 1 month or more with the enzyme action of koji mold before and after fermentation and aging for a short period of about 5 to 10 days mainly at a high temperature of about 50 ℃ due to the enzyme action of koji mold However, in any case, since 5 to 13% of sodium chloride is contained, its use is limited mainly to cooking of Japanese food such as miso soup and miso pickles.
In addition, in the above-mentioned production method in which fermentation and aging are performed at a relatively low temperature of about 30 ° C., lactic acid bacteria and yeasts grow abnormally, cause abnormal fermentation, resulting in quality deterioration, or springing during storage, etc. Cause inconvenience. In addition, as described in P70, P71 and P116 of the “New Miso Technology Handbook” published on March 15, 2006, there is no problem when the salt content is high. In the production of relatively small amounts of 5-7%, heat-resistant lactic acid bacteria and Bacillus can be produced even at the high temperature of around 50 ° C. even when the growth of lactic acid bacteria and yeasts is inhibited and the degradation of the above-mentioned enzymes mainly contained in cocoons is mainly aged. It has been reported that rancidity and rot occur due to abnormal growth of heat-resistant bacteria such as systems.
On the other hand, in recent years, unsalted or low-salted salt has been developed for the purpose of expanding the use of the fruit, maintaining the health of the general public, or for patients with hypertension, kidney disease, etc. where salt intake is prohibited or restricted. Are known. For example, the following Patent Document 1 discloses an invention relating to a functional composition mainly composed of an unsalted or low-salt miso and an extract thereof, which are suitable for patients with kidney diseases and can be used for various foods. Has been.
Patent Document 2 below includes a step of immersing the cereal in water for 1 hour, a step of boiling the cereal in water until the cereal becomes soft, a step of crushing the cereal, and adding koji mold to the crushed cereal And the manufacturing method of the salt-free salt which consists of the process of aging in a room | chamber or refrigerated is disclosed.
P70, P71 and P116 of “New Miso Technology Handbook” issued on March 15, 2006 JP 2006-70015 JP Japanese Patent Laid-Open No. 2004-215626

しかし乍ら、特許文献1の実施例1〜4によれば、大豆及び麹を混合した混合物を50℃24時間の発酵分解させることにより無塩みそを製造したものが記載されているが、上記の非特許文献1の記載から明らかなように、食塩を5〜7%含有する味噌の製造においてすら、乳酸菌による酸敗や酵母による湧きを生ずることに鑑み、食塩5%以下の減塩や無塩みその製造においても、かゝる不都合を生ずる惧れがある。本願の発明の多くの試験研究によれば、仕込用配合物に多くの雑菌を含み、仕込み諸味中に増殖し、また、45〜65℃の高温で熟成させても耐熱性細菌が増殖して熟成物の酸敗を生じ、更には、熟成後常温になった後、増殖し、酸敗などの品質劣化を生じ、保存ができないことが判明した。
特許文献2の発明は、穀類を麹と配合する前に、上記のような処理工程を必要とし、作業が煩わしく非能率であるばかりでなく、その配合物を室温又は冷蔵して熟成させる。実施例では、該配合物を4℃〜35℃程度で24〜48時間熟成することにより無塩みそを製造することが記載されているが、麹の有するプロテアーゼ及びアミラーゼによる分解作用に適した温度より著しく低い温度であるから、48時間程度ではこれらの酵素作用が殆ど働かないので、蛋白質及び澱粉分解率及び溶解率の著しく低下した製品となる不都合を回避できない。
而も、上記従来の方法で製造された無塩又は低塩みそは、みそ特有の発酵臭があるので、みそ汁以外の特に、洋風の調味料としての利用には適さない。
本発明は、かゝる従来技術の課題を解消し、その製造工程数は従来一般のみそ醸造法における工程と変わらず、而も、仕込用配合物を容器に仕込んだときから、混入している細菌の増殖を抑制し得られ、且つ主として麹の有するプロテアーゼ及びアミラーゼによる分解作用により、約45〜65℃の高温で熟成させる過程において、耐熱性細菌、その他のバクテリアなどを含む一般生菌数を著しく減少せしめることができ、安定した而も従来の無塩又は低塩のみそ特有の発酵臭のない而もみその旨みを有すると共に、爽やかな酸味と風味を有する無塩又は低塩のみそ系調味料熟成物を短時間に且つ高能率に得られ、更には、従来使用されている各種保存料を添加することなく、そのまま保存し得る無塩又は低温のみそ系調味料の製造法とその製品を提供することを目的とする。
However, according to Examples 1 to 4 of Patent Document 1, although a mixture of soybeans and koji is produced by fermenting and decomposing a mixture of soybeans and koji at 50 ° C. for 24 hours, As is apparent from the description of Non-Patent Document 1, the production of miso containing 5 to 7% of sodium chloride, even in the case of producing rancidity by lactic acid bacteria and springing by yeast, reduced salt or salt-free of 5% or less of sodium chloride There is a possibility that such inconvenience may be caused in the manufacture of the fruit. According to many test studies of the invention of the present application, the preparation mixture contains many germs and grows in the prepared moromi, and the heat-resistant bacteria grow even when aged at a high temperature of 45 to 65 ° C. It turned out that the aging of the ripened product was caused, and further, after aging, it reached normal temperature and then proliferated, resulting in quality deterioration such as rancidity, and could not be stored.
The invention of Patent Document 2 requires the processing steps as described above before blending cereals with straw, and is not only cumbersome and inefficient, but also ripens the blend at room temperature or refrigerated. In the Examples, it is described that the unsalted miso is produced by aging the blend at about 4 ° C. to 35 ° C. for 24 to 48 hours. However, the temperature suitable for the decomposition action by protease and amylase of koji. Since the temperature is much lower, these enzyme actions hardly work after about 48 hours, and the inconvenience of becoming a product in which the protein and starch degradation rate and dissolution rate are significantly reduced cannot be avoided.
However, unsalted or low-salt miso produced by the above-mentioned conventional method has a fermentation odor peculiar to miso, and is not suitable for use as a seasoning for Western-style seasonings other than miso soup.
The present invention eliminates such problems of the prior art, and the number of manufacturing steps is the same as that in the conventional general brewing method. The number of general viable bacteria, including heat-resistant bacteria and other bacteria, in the process of aging at a high temperature of about 45 to 65 ° C., due to the degradation action by protease and amylase, which is mainly possessed by sputum, The salt-free or low-salt miso system has a refreshing acidity and flavor, and has a savory taste that does not have a fermentation odor peculiar to the conventional salt-free or low-salt fish. A method for producing a salt-free or low-temperature miso-based seasoning which can obtain a seasoning aging product in a short time and with high efficiency, and can be stored as it is without adding various conventionally used preservatives. Product The purpose is to provide.

本発明は、請求項1に記載の通り、蒸煮大豆、みそ用麹、0〜5%の食塩及び種水から成る配合物に少なくとも1種の食用有機酸を添加し、混合したものを容器内に仕込み、45〜65℃の温度で熟成せしめることを特徴とする無塩又は低塩のみそ系調味料の製造法に存する。
更に本発明は、上記の調味料の製造法において、クエン酸、乳酸、酢酸、リンゴ酸、コハク酸、酒石酸から成る群から選択した1種又は2種以上の食用有機酸を該配合物に添加することを特徴とする。
更に本発明は、該みそ用麹に代えクエン酸生成麹を蒸煮大豆、0〜5%の食塩及び種水とから成る配合物を、食用有機酸を添加することなく、混合したものを容器内に仕込み、45〜65℃の温度で熟成せしめることを特徴とする無塩又は低塩のみそ系調味料の製造法に存する。
更に本発明は、上記の配合物に少なくとも1種の食用有機酸を添加し、混合したものを容器内に仕込み、45〜65℃の温度で熟成せしめることを特徴とする。
更に本発明は、上記の各製造法により得られた無塩又は低塩のみそ系調味料に係る。
According to the present invention, as described in claim 1, at least one edible organic acid is added to a composition comprising steamed soybeans, miso koji, 0-5% salt and seed water, and the mixture is mixed in a container. And a ripening at a temperature of 45 to 65 ° C., which is a method for producing a salt-free or low-salt miso seasoning.
Furthermore, the present invention provides the above-mentioned seasoning production method, wherein one or more edible organic acids selected from the group consisting of citric acid, lactic acid, acetic acid, malic acid, succinic acid, and tartaric acid are added to the blend. It is characterized by doing.
Furthermore, the present invention provides a mixture of a mixture comprising steamed soybean, 0-5% salt and seed water in place of the miso koji, without adding edible organic acid. And a ripening at a temperature of 45 to 65 ° C., which is a method for producing a salt-free or low-salt miso seasoning.
Furthermore, the present invention is characterized in that at least one edible organic acid is added to the above-mentioned blend, and the mixture is charged into a container and aged at a temperature of 45 to 65 ° C.
Further, the present invention relates to a salt-free or low-salt miso seasoning obtained by each of the above production methods.

請求項1に係る発明によれば、上記の仕込まれる配合物に少なくとも1種の食用有機酸を添加したので、食塩が5%以下又は無塩の仕込用配合物であっても、これに混入している耐熱性細菌その他の一般細菌数の増殖が抑制され、安心して良好な仕込み作業が可能となり、その後、45〜65℃の高温で熟成せしめるので、一般の細菌はもとより、耐熱性細菌を死滅させる効果が増大し、一般生菌数を著しく減少し得られると同時に、みそ用麹の有するプロテアーゼやアミラーゼによる蛋白質、澱粉の分解を良好に行うことができ、最短で1日、所望により2〜10日程度の短期間で発酵臭がなく而もみその旨みを有すると共に、添加した食用有機酸による爽快な酸味が付与された無塩又は低塩のみそ系調味料が得られる。更には、その有機酸の含有により従来の保存料を添加することなく保存することができる製品が得られる。
而して、本発明のみそ系調味料は、上記の特性を有するので、その用途として、従来と同様に、味噌汁の他、例えば、洋風の各種のドレッシング、ソースなどの各種調理に利用でき、更には、腎臓患者などの食用とした場合に、爽快な風味を与えることができ、従来の無塩又は低塩みそに比し、利用範囲を拡大することができる。
食用有機酸としては、クエン酸、乳酸、酢酸等の単独又は混合物は、上記の請求項1に記載の効果をもたらすことに加え、夫々の有機酸特有の爽快な風味を有するみそ系調味料を製造することができる。
また、請求項3に記載のように、クエン酸生成麹を該配合物に添加するときは、クエン酸生成麹にはその製麹中に生成したクエン酸が含まれるので、別個に、食用有機酸を添加する必要がなく、上記の微生物抑制効果をもたらす。また、クエン酸生成麹菌は、みそ用麹菌とは異なり、特に耐酸性に優れた分解酵素を持っているので、蛋白質及び澱粉分解率が向上し、更に食感の滑らかなみそ系調味料が得られる。
更に本発明は、請求項4に記載のように、クエン酸生成麹の使用下で、少なくとも1種の食用有機酸を添加するときは、後記に明らかにするように、みそ用麹の使用下での食用有機酸の添加量より少ない添加量で同時に良好なみそ系調味料が得られる。
上記の製造法により得られた製品は、食塩含有量が0〜5%であり、発酵臭がなく而も爽やかな酸味、風味を有するため、上記したように、一般人には勿論、腎臓疾患その他の患者用に適すると共に、みそ汁などの和食や中華料理用の調味料としてばかりでなく、各種のドレッシング、スープ類などの洋風の調味料として利用し、従来の無塩又は減塩みそとは異なる利用価値の高い新規なみそ系調味料として提供できる。
According to the invention according to claim 1, since at least one edible organic acid is added to the above-mentioned preparation, even if it is a preparation for preparation of salt of 5% or less or salt-free, The growth of the number of heat-resistant bacteria and other general bacteria is suppressed, and good preparation work can be performed with peace of mind.After that, aging is performed at a high temperature of 45 to 65 ° C. The effect of killing can be increased, and the number of general viable bacteria can be significantly reduced. At the same time, the protein and starch can be satisfactorily decomposed by protease and amylase of miso soup. A salt-free or low-salt miso-based seasoning is obtained in a short period of about ˜10 days, with no fermented odor and umami taste, and a refreshing acidity imparted by the added edible organic acid. Furthermore, the product which can be preserve | saved without adding the conventional preservative by the inclusion of the organic acid is obtained.
Thus, the miso-based seasoning of the present invention has the above-mentioned characteristics, and as its use, in addition to miso soup, for example, it can be used for various dishes such as various Western-style dressings and sauces, Furthermore, when used as an edible for kidney patients or the like, a refreshing flavor can be imparted, and the range of use can be expanded compared to conventional salt-free or low-salt miso.
As the edible organic acid, citric acid, lactic acid, acetic acid and the like alone or in mixture, in addition to bringing about the effect of claim 1, the miso seasoning having a refreshing flavor peculiar to each organic acid. Can be manufactured.
Further, as described in claim 3, when the citric acid-producing koji is added to the blend, the citric acid-producing koji contains the citric acid produced during the koji making. It is not necessary to add an acid, and the above-mentioned microorganism suppressing effect is brought about. In addition, citrate-producing gonococci, unlike miso gonococci, have a degrading enzyme with particularly excellent acid resistance, so that the rate of protein and starch degradation is improved, and a smooth miso seasoning is obtained. It is done.
Furthermore, as described in claim 4, when adding at least one edible organic acid under the use of a citric acid-producing cocoon, as described in claim 4, the use of a miso cocoon as described below. At the same time, a good miso-based seasoning can be obtained with an addition amount smaller than the addition amount of the edible organic acid.
The product obtained by the above production method has a salt content of 0-5%, has no fermentation odor, and has a refreshing sourness and flavor. It is suitable not only as a seasoning for Japanese and Chinese dishes such as miso soup, but also as a Western-style seasoning such as various dressings and soups, and is different from conventional unsalted or low-salt miso. It can be provided as a new miso seasoning with high utility value.

本発明の無塩又は低塩のみそ系調味料の製造法の実施形態例につき、以下詳述する。
仕込用配合原料は、大豆と米、麦、豆から選んだ原料に常法により麹菌を種付けして製麹したみそ用麹と0〜5%の食塩と種水を夫々所望の割合で配合して成る通常の仕込用配合物に、本発明によれば、更に仕込用の配合原料として、例えば、クエン酸、乳酸、酢酸などの各種食用有機酸の少なくとも1種又は2種以上の食用有機酸を所定量添加、混合したものを仕込用原料とする。かゝる食用有機酸を含有する配合物を容器内に仕込むことにより、その仕込んだ、いわゆる諸味に含有する一般細菌の増殖が該有機酸により抑制されるばかりでなく、この諸味を45〜65℃、好ましくは、50〜60℃の高温で、例えば、1〜10日間程度の短時間熟成せしめることにより、含有する或いは外部から侵入した耐熱性細菌を含む一般細菌の生育を抑制し、一般生菌数を著しく少なくできるので、酸敗や腐敗を防止したみそ系調味料が得られる。
一方、同時にみそ麹の有するプロテアーゼ、アミラーゼ、その他各種の酵素により、原料成分は分解し、ペプチド、アミノ酸、ブドウ糖などを生ずると共に、生成物の化学的な分解合成により、みそらしい光沢、旨味、香味、組織を醸成された熟成物を生ずるが、本発明では、有機酸を含有するので、その熟成物は、いわゆるみその有する発酵臭がなく、爽快な酸味を有するみそ系調味料として得られ、更には、その得られた製品を常法により包装し、保存したとき、有機酸は保存剤として働き、長期に亘り保存でき、別個に保存剤を添加する必要がない。
尚、仕込用配合物中の食塩の含有量0〜5%に対して各種有機酸の仕込用配合物に対する添加量は、食塩の含有量が0%〜5%に増大するに伴ない、少ない量で済む。例えば、食塩0%の場合は、有機酸の添加量は約2%であれば足りる。これ以上添加量を増大しても良いが、経済的な検知からみて、この程度にとどめることが有利である。
本発明によれば、通常使用されているみそ用麹に代えて、製麹中、クエン酸を生成する麹菌を用いて製麹したクエン酸生成麹を仕込用配合原料として使用できる。該クエン酸生成麹はクエン酸を含有するので、以下の試験例で明らかにするように、仕込用配合原料として別個に有機酸を必ずしも添加配合する必要がない。
また、クエン酸生成麹の有する各種酵素は、耐酸性であるので、みそ用麹を用いた場合に比し、熟成度の向上したみそ系調味料が得られることが判った。
Embodiments of the method for producing a salt-free or low-salt miso seasoning according to the present invention will be described in detail below.
The raw materials used for preparation are blended in the desired proportions with miso koji made from soybeans, rice, wheat, and beans, seeded with koji molds by conventional methods, and 0-5% salt and seed water. According to the present invention, according to the present invention, as an additional raw material for preparation, for example, at least one or more kinds of edible organic acids such as citric acid, lactic acid, acetic acid, etc. A predetermined amount is added and mixed as a raw material for charging. By charging a compound containing such an edible organic acid in a container, not only the growth of general bacteria contained in the so-called moromi, which is charged, is suppressed by the organic acid, but also this moromi is reduced by 45 to 65. C., preferably 50 to 60.degree. C., for example, by aging for a short time of about 1 to 10 days, to suppress the growth of general bacteria including heat-resistant bacteria contained or invaded from the outside. Since the number of bacteria can be remarkably reduced, a miso seasoning that prevents acid spoilage and spoilage can be obtained.
At the same time, protease, amylase, and other various enzymes in miso are decomposed into raw materials, producing peptides, amino acids, glucose, etc., and chemical degradation and synthesis of the product, resulting in a brilliant luster, umami, and flavor. In the present invention, since the organic acid is contained in the present invention, the mature product is obtained as a miso-based seasoning having a sour taste without a so-called fermentation odor. Furthermore, when the resulting product is packaged and stored in a conventional manner, the organic acid acts as a preservative and can be stored for a long period of time without the need to add a preservative separately.
In addition, with respect to the salt content 0-5% in the preparation composition, the amount added to the preparation composition of various organic acids is small as the salt content increases from 0% to 5%. Just do it. For example, when the salt is 0%, the amount of organic acid added is about 2%. Although the amount added may be increased further, it is advantageous to keep it to this level in view of economical detection.
According to the present invention, instead of the commonly used miso koji, a citric acid-producing koji produced using koji molds that produce citric acid during koji making can be used as a blending raw material. Since the citric acid producing soot contains citric acid, it is not always necessary to separately add and mix an organic acid as a blending raw material for preparation, as will be apparent from the following test examples.
Further, since various enzymes contained in the citric acid-producing koji are acid-resistant, it has been found that miso-based seasonings having an improved maturity can be obtained as compared with the case of using koji for miso.

次に、本発明の実施例を従来例と対比した比較試験例につき詳述する。
比較試験例1
原料米は一晩浸漬後、水切りし、次いでこれを甑(こしき)で蒸気吹き付け後、30分蒸煮し、これを送風機で35℃程度まで冷却し、蒸し米を得た。この蒸し米にみそ用麹菌としてAsp. oryzae(アスペルギリス オリゼー)と、Asp. awamori(アスペルギリス アワモリ)などのクエン酸を生成する麹菌(以下、クエン酸生成麹菌と略称)を夫々種付けして、夫々、30〜40℃で2日間かけて製麹した。
一方、原料大豆は洗浄し、一晩浸漬した後、水切りし、次いで、オートクレーブで0.75Kg/cm2で15分蒸し、得られた蒸し豆を30℃まで冷却した後、5m/mのチョッパーで擂砕した。
Next, an example of the present invention will be described in detail with respect to a comparative test example in comparison with a conventional example.
Comparative test example 1
The raw rice was soaked overnight, drained, then steamed with a koji, steamed for 30 minutes, and cooled to about 35 ° C. with a blower to obtain steamed rice. Assorted Aspergillus oryzae (Aspergilis oryzae) and Asp. Awamori (Aspergilis awamori) such as Aspergillus oryzae as the miso for this steamed rice, Each of them was smelted at 30 to 40 ° C. for 2 days.
On the other hand, the raw soybeans are washed, soaked overnight, drained, then steamed in an autoclave at 0.75 Kg / cm 2 for 15 minutes, and the obtained steamed beans are cooled to 30 ° C., and then with a 5 m / m chopper. Crushed.

次いで、上記の仕込用原料と種水とを、下記表1に示す夫々の仕込用原料を配合した4種類の無塩の仕込用配合物を調製し、これらの仕込用配合物を夫々の容器に仕込み、55℃で2日間熟成させた。下記表1から明らかなように、対照区1は、従来の無塩みそ製造用の仕込用配合物である。テスト区1〜3は夫々本発明の実施例の仕込用配合物を示し、テスト区1は、食用有機酸としてクエン酸2%を配合した仕込用配合物であり、テスト区2は、みそ用麹に代え、クエン酸生成麹を配合し、且つクエン酸を1%配合して成る仕込用配合物であり、テスト区3は、クエン酸生成麹を20歩配合し、クエン酸無添加の仕込用配合物である。   Next, four kinds of salt-free preparations were prepared by blending the above-mentioned raw materials for charging and seed water with the respective raw materials for charging shown in Table 1 below. And aged at 55 ° C. for 2 days. As is clear from Table 1 below, Control Group 1 is a conventional blend for preparing salt-free miso. Test groups 1 to 3 show the preparations for preparation of the examples of the present invention, respectively. Test group 1 is a preparation for preparation containing 2% citric acid as an edible organic acid, and test group 2 is used for miso It is a formulation for charging that contains 1% citric acid in addition to citric acid-producing soot instead of koji, and Test Zone 3 is a 20-step mix of citric acid-producing koji, with no addition of citric acid Formulation.

Figure 2009000065
Figure 2009000065

上記表1に示す夫々の仕込用配合物の熟成物の分析並びに官能テスト結果は、下記表2に示す通りであった。   Table 2 below shows the results of analysis and sensory test of the aging products of the respective preparation formulations shown in Table 1 above.

Figure 2009000065
Figure 2009000065

表1及び表2から明らかなように、無塩の仕込み用配合物において、対照区の熟成物は、従来の無塩みそに相当するが、一般生菌数が多く酸敗臭があり、異常発酵が確認された。クエン酸を添加したテスト区1の熟成物は、一般生菌数が300以下/gとなり、有機酸による微生物制御作用が認められると共に、発酵臭がなく、爽快な酸味を有し、而も、みそ特有の旨味を有するみそ系調味料として得られた。
テスト区2は、みそ用麹10歩に代え、クエン酸生成麹を10歩配合し、クエン酸の添加量を1%と、テスト区1の半分の量としたものであるが、テスト区1と同様に有機酸による微生物制御作用を有し、同様のみそ系調味料として得られた。このことは、クエン酸生成菌10歩はクエン酸1%弱の添加量に相当し、これにより、一般細菌(耐熱性細菌を含む)の制御作用を発揮すると考えられる。テスト区3は、クエン酸生成麹の麹歩合をテスト区3の2倍の20歩用いれば、別個にクエン酸を添加しないでも、そのクエン酸生成麹の含有する約2%弱のクエン酸がテスト区2と同様に微生物制御作用を発揮し、良好なみそ系調味料として得られることが判った。
As is clear from Table 1 and Table 2, in the salt-free formulation, the matured product in the control group is equivalent to the conventional salt-free miso, but it has a large number of general viable bacteria and has an acid odor and abnormal fermentation. Was confirmed. The aged product of test section 1 to which citric acid was added had a general viable cell count of 300 or less / g, a microbial control action by organic acid was observed, there was no fermentation odor, and it had a refreshing acidity, It was obtained as a miso-based seasoning having a unique taste of miso.
In Test Zone 2, instead of 10 steps for miso soup, 10 steps of citric acid-producing soot were added and the amount of citric acid added was 1%, which was half that of Test Zone 1. In the same way as above, it has a microbial control action by an organic acid, and it was obtained as a seasoning. This means that 10 steps of citric acid-producing bacteria correspond to an addition amount of citric acid of less than 1%, and this is considered to exert a control effect on general bacteria (including heat-resistant bacteria). In test group 3, if 20% of the citrate production rate is used, which is twice that of test group 3, about 2% less citric acid contained in the citrate production rate can be obtained without adding citric acid separately. It was found that, as in Test Zone 2, it exerts microbial control action and can be obtained as a good miso seasoning.

試験例
次に、本発明の実施例として、クエン酸に代え、乳酸、酢酸を無塩の仕込用配合物に配合して成る仕込用配合物を下記表3に示すテスト区4〜7の4種類の仕込用配合物を調製し、夫々の有機酸による微生物制御効果について試験した。
更に詳細には、下記表3に示すように、テスト区4は、従来の無塩みそ製造用の仕込用配合物に乳酸を1%添加して成る本発明の実施例の仕込用配合物、テスト区5は、従来の無塩みそ製造用の仕込用配合物に酢酸を0.4%添加して成る本発明の実施例の仕込用配合物、テスト区6は、みそ用麹に代え、クエン酸生成麹を用い、且つ乳酸を0.5%添加して成る本発明の実施例の仕込用配合物、テスト区7は、みそ用麹に代え、クエン酸生成物を用い、且つ酢酸を0.2%添加して成る本発明の実施例の仕込用配合物である。而して、これら4種類の仕込用配合物を夫々の容器に仕込み、55℃で2日間熟成させた。
Test Example Next, as an example of the present invention, instead of citric acid, a charging composition formed by blending lactic acid and acetic acid into a salt-free charging composition is shown in 4 in Test Groups 4 to 7 shown in Table 3 below. A variety of feed formulations were prepared and tested for microbial control effects with each organic acid.
More specifically, as shown in Table 3 below, the test section 4 is a preparation for charging according to an example of the present invention, in which 1% lactic acid is added to a conventional preparation for producing salt-free miso, Test section 5 is a preparation for preparation of an example of the present invention obtained by adding 0.4% of acetic acid to a conventional preparation for producing salt-free miso, test section 6 is replaced with miso soup, citric acid The feed composition of the embodiment of the present invention using test product 7 and adding 0.5% lactic acid, test group 7 uses a citric acid product and 0.2% acetic acid instead of miso product. It is the preparation for preparation of the Example of this invention which consists of. Thus, these four kinds of preparations were charged into each container and aged at 55 ° C. for 2 days.

Figure 2009000065
Figure 2009000065

これら4種類の仕込用配合物の仕込み直後、熟成2日後及び熟成後30℃で1週間放置後の一般生菌数の測定結果を下記表4に示す。   Table 4 below shows the results of measurement of the number of viable bacteria immediately after charging these four kinds of preparations, after 2 days of aging, and after standing for 1 week at 30 ° C. after aging.

Figure 2009000065
Figure 2009000065

下記表5は、これら4種類の仕込用配合物の55℃で2日間熟成後の分析並びに官能テストの結果を示す。   Table 5 below shows the analysis and sensory test results of these four feed formulations after aging at 55 ° C. for 2 days.

Figure 2009000065
Figure 2009000065

上記表3〜表5から明らかなように、テスト区4〜7の夫々の仕込用配合原料に混入している一般細菌は、夫々添加されている乳酸、酢酸の各有機酸により、著しい微生物抑制効果を受け、熟成後の一般生菌数は著しく減少し、食感、風味良好な熟成物をもたらす。因みに、対照区1の仕込用配合物の仕込直後の一般生菌数は、106オーダー/gであった。
尚また、夫々の熟成物を熟成後、30℃で1週間放置しておいても、一般細菌が増殖することがなく、良好な品質を維持し、有機酸を含有することにより、保存性が付与され、特に保存剤を添加する必要がない。
尚、クエン酸生成麹を使用した場合は、含有するクエン酸と乳酸、酢酸との併用により、微生物抑制効果をもたらすばかりでなく、乳酸、酢酸の添加量をみそ用麹使用の場合における半分の量で済むことが判った。
結局、本発明の実施例であるテスト区4〜7の仕込用配合物が示すように、無塩の仕込用配合物であっても、乳酸、酢酸の単独又はクエン酸との併用により、酸敗を防止し、而も、みその旨味を有するが、その発酵臭がない酸味を有する洋風の調味料にも適した、而も長期保存の効くみそ系調味料として得られることが確認された。
As is clear from Tables 3 to 5 above, the general bacteria mixed in each of the feed ingredients in the test sections 4 to 7 are markedly microbial controlled by the added organic acids of lactic acid and acetic acid, respectively. Due to the effect, the number of general viable bacteria after aging is remarkably reduced, resulting in a ripened product having a good texture and flavor. Incidentally, the number of general viable bacteria immediately after the preparation of the preparation for the control group 1 was 10 6 orders / g.
In addition, after aging each aged product, even if it is allowed to stand at 30 ° C. for 1 week, general bacteria do not grow, maintain good quality, and contain organic acids, so that the storage stability is improved. It is necessary to add a preservative.
In addition, when citric acid-producing cocoons are used, the combined use of citric acid with lactic acid and acetic acid not only brings about a microbial control effect, but also the amount of lactic acid and acetic acid added is half that in the case of using miso soup. I found that it was enough.
Eventually, as shown in the examples 4 to 7 in the test examples of the present invention, even in the case of a salt-free preparation, the acid loss was caused by lactic acid, acetic acid alone or in combination with citric acid. It has been confirmed that it can be obtained as a miso-based seasoning which is suitable for a Western-style seasoning having a sour taste but having a fermented odor, and having a sour taste.

試験例3
5%以下の食塩を添加した仕込用配合物を熟成させる場合の有機酸添加効果を試験するべく、下記表6及び表7に示す仕込用配合物を調製し、これらの7種類の仕込用配合物を夫々の容器に仕込み、55℃で2日間熟成させた。
対照区2及び対照区3は、食塩3%及び5%を夫々添加した従来の低塩みそ製造用の仕込用配合物である。テスト区8,9,10は、食塩3%を添加した対照区2に対応する仕込用配合物に有機酸を添加した本発明の実施例の仕込用配合物、テスト区11は、食塩5%を添加した対照区3に対応する仕込用配合物に有機酸を添加した本発明の実施例の仕込用配合物、テスト区12は、従来のみそ用麹に代え、これと同歩合のクエン酸生成麹を使用したが、有機酸を添加しない本発明の実施例の仕込用配合物である。
Test example 3
In order to test the effect of organic acid addition when aging a preparation for addition of 5% or less of salt, preparation preparations shown in Table 6 and Table 7 below were prepared, and these seven kinds of preparations for preparation were prepared. The product was placed in each container and aged at 55 ° C. for 2 days.
Control group 2 and control group 3 are conventional blends for the production of low-salt miso, with 3% and 5% salt added respectively. Test group 8, 9, 10 is a preparation for preparation of an example of the present invention in which an organic acid was added to a preparation for preparation corresponding to control group 2 to which 3% of salt was added, test group 11 was 5% of sodium chloride The feed formulation of the example of the present invention, in which an organic acid was added to the feed formulation corresponding to the control group 3 to which the control group 3 was added, the test group 12, instead of the conventional koji, the citric acid at the same rate as this It is the preparation for preparation of the Example of this invention which uses the produced soot but does not add an organic acid.

Figure 2009000065
Figure 2009000065

Figure 2009000065
Figure 2009000065

これら7種類の仕込用配合物の仕込み直後、熟成2日後及び熟成後30℃で1週間放置後の一般生菌数の測定結果を下記表8,9に示す。   Tables 8 and 9 below show the results of measurement of the number of general viable bacteria immediately after charging these 7 kinds of preparations, after 2 days of aging and after standing for 1 week at 30 ° C. after aging.

Figure 2009000065
Figure 2009000065

Figure 2009000065
Figure 2009000065

下記表10,11は、これら7種類の仕込用配合物の55℃で2日間熟成後の分析並びに官能テストの結果を示す。   Tables 10 and 11 below show the results of analysis and sensory test after aging for 2 days at 55 ° C. for these 7 preparation formulations.

Figure 2009000065
Figure 2009000065

Figure 2009000065
Figure 2009000065

上記表6〜表11から明らかなように、テスト区8〜12の夫々の仕込用配合原料に混入している一般細菌は、夫々添加されている夫々有機酸により、著しい微生物抑制効果を受け、熟成後の一般生菌数は著しく減少し、食感、風味良好なみそ系調味料をもたらす。
尚また、夫々の熟成物を熟成後、30℃で1週間放置しておいても、一般細菌が増殖することがなく、良好な品質を維持し、有機酸を含有することにより、保存性が付与され、特に保存剤を添加する必要がない。
尚、クエン酸生成麹を使用した場合は、別個に有機酸を添加しないでも、微生物抑制効果をもたらすことが判った。
結局、本発明の実施例であるテスト区8〜12の仕込用配合物が示すように、食塩5%以下の無塩の仕込用配合物であっても、クエン酸、乳酸、酢酸の単独又は乳酸、クエン酸との併用により、酸敗を防止し、而も、みその旨味を有するが、その発酵臭がない酸味を有する洋風の調味料にも適した、而も長期保存の効くみそ系調味料として得られることが確認された。
As is apparent from Tables 6 to 11 above, the general bacteria mixed in the raw materials for preparation in each of the test sections 8 to 12 receive a significant microbial inhibitory effect by the respective organic acids added, The number of general viable bacteria after aging is significantly reduced, resulting in miso-based seasonings with a good texture and flavor.
In addition, after aging each aged product, even if it is allowed to stand at 30 ° C. for 1 week, general bacteria do not grow, maintain good quality, and contain organic acids, so that the storage stability is improved. It is necessary to add a preservative.
In addition, when a citric acid production | generation tank was used, even if it did not add an organic acid separately, it turned out that the microorganisms inhibitory effect is brought about.
Eventually, as shown in the preparations of test sections 8 to 12 which are examples of the present invention, even salt-free preparations containing 5% or less of salt, citric acid, lactic acid, acetic acid alone or Combined use with lactic acid and citric acid prevents spoilage and is suitable for Western-style seasonings with sour taste but no fermented odor. It was confirmed that it can be obtained as a fee.

尚、上記のテスト区1〜12の実施例から明らかなように、有機酸の添加量は、食塩の添加量が増大すれば、これに反比例して少なくて済む。多くの試験研究の結果、無塩又は低塩の仕込用配合物を45〜65℃で1〜10日間の短期間で仕込み時から熟成まで有機酸による耐熱性細菌などの微生物の増殖を抑制し、旨味と酸味を有する良質の本発明の新規なみそ系調味料が得られることが確認された。無塩の仕込用配合物への有機酸の添加量は、クエン酸、乳酸、酢酸の各単独の場合、2%又はそれ以下で充分であり、クエン酸生成麹は、生成クエン酸含有量約2%に対応する麹歩合20%で足りる。これらの各酸又はクエン酸生成麹との併用の場合は、各有機酸の添加量はそれ以下でよいことが判った。
勿論、経済的見地を度外視すれば、上記の添加量以上添加してもよい。但し、みそ麹を使用する場合は、有機酸によるみそ用麹の酵素の蛋白質、澱粉を分解する性能の低下を予め考慮し、上記の高温で1〜10日の短期間で熟成できるように有機酸の添加量を決定することが好ましい。
As is clear from the examples of test sections 1 to 12, the amount of organic acid added can be reduced in inverse proportion to the amount of salt added. As a result of many researches, the growth of microorganisms such as heat-resistant bacteria such as heat-resistant bacteria due to organic acids is suppressed from the time of preparation to maturation in a short period of 1 to 10 days at 45 to 65 ° C. It was confirmed that a novel miso seasoning of the present invention having good taste and sourness can be obtained. In the case of each of citric acid, lactic acid, and acetic acid, 2% or less is sufficient for the addition amount of the organic acid to the salt-free feed formulation. A yield rate of 20% corresponding to 2% is sufficient. In the case of the combined use with each of these acids or citric acid producing soot, it was found that the amount of each organic acid added may be less than that.
Of course, if the economic point of view is not taken into consideration, it may be added in an amount greater than the above-mentioned addition amount. However, when using miso koji, take into account the decline in the ability to decompose the protein and starch of the miso koji enzyme by organic acids in advance, so that it can be matured in a short period of 1 to 10 days at the above high temperature. It is preferable to determine the amount of acid added.

尚、食用有機酸として、上記実施例で使用したクエン酸、乳酸、酢酸の他のリンゴ酸、コハク酸、酒石酸でも同様の作用効果をもたらす。   As the edible organic acid, citric acid, lactic acid, acetic acid, other malic acid, succinic acid, and tartaric acid used in the above-described examples also bring about the same effect.

次に、試験例1のテスト区2を用い、55℃で2日間熟成させて得られた無塩のみそ系調味料(以下“調味料”と略称する)を使って各種料理を下記のように調理した。
調理例1
ビーフシチュー(10人分)
牛バラ肉 1Kg
デミグラスソース 1Kg
調味料 500g
赤ワイン 適量
玉ねぎ 1個
人参 1個
ブイヨン 1000ml
付け合せ野菜 適量
作り方:肉はフライパンで表面を焼く。赤ワインのアルコールを火をつけて飛ばす(フランベ)。鍋にデミグラスソース、ブイヨン、熟成調味料、炒めた玉ねぎ、人参を入れ火にかけて、肉を入れて煮込む。灰汁を取りながら3時間煮込んで出来上がり。
調理例2
トマトのみそ汁(3人分)
トマト中玉 2個(缶詰のホールトマトでも良い)
調味料 40g
水 2カップ
固形ブイヨン 1個
作り方:トマトを一口サイズに切り分ける。鍋に水、ブイヨン、トマト切り分けたものを入れ煮込み、火が通ったところで、熟成調味料を入れ沸騰したら出来上がり。
調理例3
トマトと春野菜のサラダ(10人分)
トマト(小) 4個
春野菜 適量
調味料 大さじ2杯
白ワインビネガー又はレモン汁 大さじ2杯
サラダ油 大さじ1杯
オリーブ油 小さじ1杯
塩、胡椒 適量
作り方:トマトを湯むきする。調味料、ビネガー、油、塩、胡椒を入れドレッシングを作る。トマト一口サイズに切り、春野菜を皿に盛り、ドレッシングをかけて出来上がり。
Next, using the test group 2 of Test Example 1, various dishes were prepared using the salt-free miso-based seasoning (hereinafter referred to as “seasoning”) obtained by aging at 55 ° C. for 2 days as follows. Cooked.
Cooking example 1
Beef stew (10 servings)
Beef rose meat 1Kg
Demigrass sauce 1Kg
Seasoning 500g
Red wine appropriate amount Onion 1 carrot 1 bouillon 1000ml
Appropriate amount of garnished vegetables How to make: Meat is grilled on a frying pan. Burn the red wine alcohol over the fire (Flambe). Put demiglace sauce, bouillon, aging seasoning, fried onion, carrots in a pan, simmer on meat, add meat and simmer. Boil for 3 hours while taking the lye.
Cooking example 2
Tomato miso soup (3 servings)
2 tomato middle balls (canned whole tomatoes are acceptable)
Seasoning 40g
2 cups of water 1 solid bouillon How to make: Cut the tomato into bite size. Put the water, bouillon, and tomatoes into a pan, simmer, put the aged seasoning and boil it.
Cooking example 3
Tomato and spring vegetable salad (for 10 people)
4 small tomatoes Spring vegetables Suitable amount Seasoning 2 tablespoons White wine vinegar or lemon juice 2 tablespoons Salad oil 1 tablespoon Olive oil 1 teaspoon Salt, pepper Seasoning, vinegar, oil, salt, pepper add dressing. Cut into a bite of tomato, serve spring vegetables on a plate, and dress it.

Claims (5)

蒸煮大豆、みそ用麹、0〜5%の食塩及び種水から成る配合物に少なくとも1種の食用有機酸を添加し、混合したものを容器内に仕込み、45〜65℃の温度で熟成せしめることを特徴とする無塩又は低塩のみそ系調味料の製造法。   At least one edible organic acid is added to a blend of steamed soybeans, miso koji, 0-5% salt and seed water, and the mixture is placed in a container and aged at a temperature of 45-65 ° C. A method for producing a salt-free or low-salt miso-based seasoning characterized by the above. クエン酸、乳酸、酢酸、リンゴ酸、コハク酸、酒石酸から成る群から選択した1種又は2種以上の食用有機酸を該配合物に添加することを特徴とする請求項1に記載のみそ系調味料の製造法。   The only system according to claim 1, wherein one or more edible organic acids selected from the group consisting of citric acid, lactic acid, acetic acid, malic acid, succinic acid and tartaric acid are added to the formulation. Seasoning production method. 該みそ用麹に代えクエン酸生成麹を蒸煮大豆、0〜5%の食塩及び種水とから成る配合物を、食用有機酸を添加することなく、混合したものを容器内に仕込み、45〜65℃の温度で熟成せしめることを特徴とする無塩又は低塩のみそ系調味料の製造法。   In place of the miso koji, citrate-producing koji is prepared by mixing a mixture of steamed soybeans, 0-5% salt and seed water into a container without adding edible organic acid, A method for producing a salt-free or low-salt miso seasoning characterized by aging at a temperature of 65 ° C. 請求項3に記載の配合物に少なくとも1種の食用有機酸を添加し、混合したものを容器内に仕込み、45〜65℃の温度で熟成せしめることを特徴とする無塩又は低塩のみそ系調味料の製造法。   At least one edible organic acid is added to the composition according to claim 3, and the mixture is placed in a container and aged at a temperature of 45 to 65 ° C. A method of manufacturing seasonings. 請求項1〜4のいずれか1つに記載の製造法により製造した無塩又は低塩のみそ系調味料の製造法。   A method for producing a salt-free or low-salt miso seasoning produced by the production method according to any one of claims 1 to 4.
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2012183006A (en) * 2011-03-04 2012-09-27 Kyoto Prefecture Method for producing pickle, and pickle
JP5523618B1 (en) * 2013-08-09 2014-06-18 ヤマサ醤油株式会社 Reduced salt bean miso with excellent taste

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0239866A (en) * 1988-07-27 1990-02-08 Mashiko Miso Kk Production of low salty miso having slight smell
JP2001190239A (en) * 2000-01-14 2001-07-17 Miyazaki Pref Gov J A Shokuhin Kaihatsu Kenkyusho Anthocyanin-containing colored sweet potato-containing miso and method for producing the same
JP2004215626A (en) * 2003-01-14 2004-08-05 Keijiro Nakamura Method for producing salt-free fermented soybean paste
JP2005013205A (en) * 2003-06-27 2005-01-20 Hiroshi Konno METHOD FOR HIGHLY PRODUCING gamma-AMINOBUTYRIC ACID BY MALTED SOYBEAN
JP2005304493A (en) * 2004-03-25 2005-11-04 Ajinomoto Co Inc Method for production of raw material for food
JP2005312439A (en) * 2004-03-31 2005-11-10 Ajinomoto Co Inc New food and method for producing the same
JP2007082441A (en) * 2005-09-21 2007-04-05 Ajinomoto Co Inc Method for producing rapidly-fermented miso-like food material having good taste and flavor

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0239866A (en) * 1988-07-27 1990-02-08 Mashiko Miso Kk Production of low salty miso having slight smell
JP2001190239A (en) * 2000-01-14 2001-07-17 Miyazaki Pref Gov J A Shokuhin Kaihatsu Kenkyusho Anthocyanin-containing colored sweet potato-containing miso and method for producing the same
JP2004215626A (en) * 2003-01-14 2004-08-05 Keijiro Nakamura Method for producing salt-free fermented soybean paste
JP2005013205A (en) * 2003-06-27 2005-01-20 Hiroshi Konno METHOD FOR HIGHLY PRODUCING gamma-AMINOBUTYRIC ACID BY MALTED SOYBEAN
JP2005304493A (en) * 2004-03-25 2005-11-04 Ajinomoto Co Inc Method for production of raw material for food
JP2005312439A (en) * 2004-03-31 2005-11-10 Ajinomoto Co Inc New food and method for producing the same
JP2007082441A (en) * 2005-09-21 2007-04-05 Ajinomoto Co Inc Method for producing rapidly-fermented miso-like food material having good taste and flavor

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2012183006A (en) * 2011-03-04 2012-09-27 Kyoto Prefecture Method for producing pickle, and pickle
JP5523618B1 (en) * 2013-08-09 2014-06-18 ヤマサ醤油株式会社 Reduced salt bean miso with excellent taste

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